High-retention-rate active edible mushroom targeting composite functional liquid and whole-linkage active-keeping preparation method and application thereof

Abstract

The application provides a high-retention-rate targeted active edible mushroom composite functional liquid and a whole-linkage active-keeping preparation method and application thereof, and belongs to the technical field of deep processing of edible mushrooms. The preparation method comprises the following steps: preparing a mixed slurry by ultrafine pulverization of edible mushroom raw materials, low-temperature negative pressure extraction of the mixed slurry, filtration sterilization of the crude extract, and sterile filling of the extract. The edible mushroom composite functional liquid prepared by the method has a high retention rate of total active ingredients, and the retention rate of active ingredients is greater than or equal to 85% after 18 months of storage at room temperature and in the dark. The application adopts low-temperature negative pressure extraction, microcrystalline vitamin C protection during the extraction process, whole-linkage nitrogen protection, and high-voltage pulse electric field sterilization technology, significantly improves the retention rate of active ingredients of edible mushrooms, fully guarantees the actual landing effect of targeted nutritional efficacy, greatly reduces the production and circulation cost, is suitable for the scene demand of large-scale staple industrialization addition, and can also be used for low-temperature foods such as yogurt and beverages.

Description

Technical Field

[0001] This invention belongs to the field of edible fungi deep processing technology, specifically relating to a high retention rate targeted active edible fungi compound functional liquid and its whole-chain activation preparation method and application. Background Technology

[0002] Edible fungi functional liquids are rich in various functional components such as cordycepin, adenosine, fungal β-glucan, and active polypeptides, possessing significant nutritional and health benefits. However, these components are all heat-sensitive and easily oxidized active substances, highly sensitive to high temperatures, oxygen, and prolonged heat retention environments. They are extremely prone to degradation, inactivation, and structural damage during extraction, sterilization, storage, and final heat processing, directly leading to a significant decrease in the product's functional activity.

[0003] Currently, the industry mostly uses 100℃ high-temperature boiling extraction and 85℃ pasteurization processes to prepare functional liquids for edible fungi. The total heat exposure during the entire process results in a loss of more than 60% of the active ingredients. In addition, the finished products produced using the above processes have an activity decay rate of more than 50% within 3 months under normal temperature storage conditions, forcing the products to rely on cold chain storage and transportation at 0~4℃, which significantly increases the cost and logistical difficulty of industrialization.

[0004] On the other hand, existing edible fungi functional liquid products, after being added to staple food systems such as rice, noodles, and porridge, generally have an activity retention rate of less than 20% after a 100℃ cooking process, making it difficult to guarantee the intended targeted nutritional efficacy. Therefore, there is an urgent need for a liveness-locking process that covers all activity loss points throughout the entire process, achieving a high retention rate of active components without the need for a cold chain, in order to meet the industrialization needs of large-scale civilian staple food addition. Summary of the Invention

[0005] The purpose of this invention is to provide a high-retention-rate targeted active edible fungi compound functional liquid, its whole-chain activation preparation method, and its application.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] A method for preparing a high-retention-rate targeted active edible fungus compound functional liquid with end-to-end activation, comprising the following steps:

[0008] S1. Mix the fruiting bodies of edible fungi and the fermentation broth of fungal mycelium, and then pulverize them into ultrafine particles until the cell walls are broken to obtain a mixed slurry.

[0009] S2. Add microcrystalline vitamin C to the mixed slurry obtained in step S1, and perform low-temperature negative pressure extraction under nitrogen protection to obtain crude extract;

[0010] S3. The crude extract obtained in step S2 is subjected to coarse filtration and fine filtration under nitrogen protection to obtain a clear extract; the extract is then placed in a high-voltage pulsed electric field for sterilization.

[0011] S4, the sterilized material liquid in step S3 is completed sterile filling under the protection of nitrogen positive pressure.

[0012] The further improvement of the technical scheme of the present application is that the mass ratio of the edible fungus fruiting body and the fungus mycelium fermentation liquor in step S1 is 1:(3-10); the edible fungus fruiting body is selected from the Cordyceps sinensis fruiting body and / or the Ganoderma lucidum fruiting body; the fungus mycelium fermentation liquor is selected from the Hericium erinaceus mycelium fermentation liquor and / or the Lentinula edodes mycelium fermentation liquor, and the mass content of the fungus mycelium in the fermentation liquor is 5%-8%.

[0013] The further improvement of the technical scheme of the present application is that the temperature control during the ultrafine grinding process in step S1 is 30-40 DEG C, and the average particle size of the mixed slurry obtained after the ultrafine grinding is ≤5 μm, which can avoid the denaturation of the mycelium protein caused by high temperature.

[0014] The further improvement of the technical scheme of the present application is that the temperature of the extraction in step S2 is 32-38 DEG C, the pressure is-0.08--0.095 MPa, and the extraction time is 30-45 min. The low-temperature environment of 32-38 DEG C can effectively avoid the thermal decomposition of the heat-sensitive protein and polysaccharide active ingredients; the negative pressure environment can make the effective active ingredients quickly precipitate, greatly shorten the extraction time, and quickly discharge the dissolved free oxygen in the extraction system under the negative pressure state, thereby reducing the probability of subsequent oxidation reaction.

[0015] The further improvement of the technical scheme of the present application is that the addition amount of the microcrystalline vitamin C in step S2 is 0.15%-0.25% of the total mass of the extraction system; and the particle size of the microcrystalline vitamin C is 200-300 nm. The microcrystalline vitamin C is uniformly dispersed in the extraction system, can in-situ capture the trace amount of oxygen and free radicals remaining in the system, can also serve as a natural hydration wrapping agent, can form a nanoscale water molecule protective film on the surface of the dissolved active molecules, and further ensures the stability of the active molecules; on the other hand, the vitamin C as an acidic protective agent can maintain the pH value of the material liquid stable in the interval of 5.5-6.5, and avoid the hydrolysis and chain breaking of the edible mushroom polysaccharide.

[0016] The further improvement of the technical scheme of the present application is that the step S3 adopts 100-200 mesh filter cloth coarse filtration and 0.2 μm ceramic membrane fine filtration.

[0017] The further improvement of the technical scheme of the present application is that the parameters of the high-voltage pulsed electric field sterilization are as follows: electric field intensity 25-35 kV / cm, pulse number 8-12, and treatment temperature not higher than 40 DEG C.

[0018] The further improvement of the technical scheme of the present application is that the oxygen content in the whole contact space of the material liquid in step S4 is ≤0.5%.

[0019] In a second aspect, the application discloses a high-retention-rate targeted active edible mushroom composite functional liquid, which is obtained by a full-linkage lock-activity preparation method using the high-retention-rate targeted active edible mushroom composite functional liquid, and the high-retention-rate targeted active edible mushroom composite functional liquid is stored in a dark environment at room temperature, and the retention rate of active ingredients is greater than or equal to 85% after being stored for 18 months.

[0020] In a third aspect, the application discloses an application of the high-retention-rate targeted active edible mushroom composite functional liquid to civilian staple food or low-temperature food, and the low-temperature food includes yogurt and / or beverage.

[0021] Due to the adoption of the technical solutions, the application has the following beneficial effects:

[0022] The application adopts low-temperature negative pressure dissolution, microcrystalline vitamin C protection in the dissolution process, full-linkage nitrogen protection, and high-voltage pulse electric field sterilization technology to prepare the high-retention-rate targeted active edible mushroom composite functional liquid. The low-temperature negative pressure dissolution can reduce the exposure time of active ingredients, the nitrogen protection can cut off the oxidation reaction path, the microcrystalline VC can remove residual reaction factors, the high-voltage pulse electric field can break the cell membrane to achieve sterilization, and an integrated lock-activity closed loop of environmental oxygen control, process inactivation, in-situ removal, and electric field sterilization is formed, so that the retention rate of active ingredients of edible mushrooms is significantly improved, and the actual landing effects of targeted nutritional efficacy and antibacterial effect are fully guaranteed.

[0023] The application uses edible fungal fruiting bodies and fermentation liquid systems with fungal mycelium as raw materials, and the fermentation supernatant is not separated and discarded, so that all nutritional substances are retained, the retention of active substances in the obtained product is more comprehensive, and the natural small-molecule peptides in the fermentation liquid can be used as solubilizers of active ingredients to effectively improve the dissolution efficiency of cordycepin and ganoderma beta-glucan.

[0024] The high-retention-rate targeted active edible mushroom composite functional liquid prepared by the application does not need cold chain storage and transportation, does not need additional artificial chemical preservatives, the retention rate of active ingredients is greater than 85% after being stored at room temperature for 18 months, the production and circulation costs are greatly reduced, the application is suitable for the scene demand of large-scale staple food industrialization, and the application can also be used for low-temperature food such as yogurt and beverage, and the active retention rate is high. DETAILED DESCRIPTION

[0025] The application will be further described below in combination with examples. It should be understood that the preferred embodiments described herein are only used to illustrate and understand the application, and are not used to limit the application.

[0026] In the following examples, the experimental methods used are conventional methods in the art unless otherwise specified.

[0027] In the following examples, the materials and reagents used are commercially available unless otherwise specified.

[0028] In the following examples, the Hericium erinaceus mycelium fermentation broth and the Lentinula edodes mycelium fermentation broth are prepared by liquid submerged fermentation technology, and the dry mycelium content of the Hericium erinaceus mycelium fermentation broth and the Lentinula edodes mycelium fermentation broth is 6.2% ± 0.3% and 5.8% ± 0.2% (measured by 105°C drying method), respectively.

[0029] The particle size of the microcrystalline vitamin C is 200-300 nm.

[0030] Example 1

[0031] This example is the preparation of a Cordyceps militaris and Hericium erinaceus targeted active composite functional liquid, which comprises the following steps:

[0032] S1, 1000g of Cordyceps militaris fruiting bodies and 5000g of Hericium erinaceus mycelium fermentation broth are mixed and then put into a low-temperature high-shear ultrafine grinder, and the whole process is carried out under the condition of temperature control at 30-40℃ to obtain a full-component broken wall mixed slurry without obvious granular feeling; the average particle size of the mixed slurry is 5μm detected by a particle size instrument.

[0033] S2, nitrogen gas with a purity of more than 99.99% is introduced into the extraction tank to replace the air in the tank, and the mixed slurry is transported into the extraction tank under the protection of nitrogen, the temperature is controlled at 35℃, the negative pressure is-0.09MPa, the nitrogen gas is continuously introduced, 12g of microcrystalline vitamin C is added, and after 40min of circulation extraction, a Cordyceps militaris and Hericium erinaceus crude extract is obtained; the detection of cordycepin retention rate is 94.2%, and the detection of Hericium erinaceus polysaccharide retention rate is 94.8%.

[0034] S3, the Cordyceps militaris and Hericium erinaceus crude extract is transported in a nitrogen sealed pipeline, first filtered through 100-200 mesh filter cloth, and then filtered through 0.2μm ceramic membrane to obtain a clear extract; the extract is pre-cooled to 20℃ and sent into a high-voltage pulse electric field sterilization, the field strength is set to 30kV / cm, the pulse number is 10, and the pulse length is 300μs, and after sterilization, the temperature of the extract does not exceed 40℃.

[0035] S4, sterile nitrogen is introduced into the sterilized blending tank to replace it to 0.5% oxygen content, and the sterilized Cordyceps militaris and Hericium erinaceus extract is transported into the blending tank under the protection of sterile nitrogen, and then filled into a sterilized brown HDPE barrel. The detection of total bacterial colony count of the product is ≤10CFU / mL, the detection of cordycepin retention rate is 92.6%, and the detection of Hericium erinaceus polysaccharide retention rate is92.2%.

[0036] After 18 months of storage at 25℃, the cordycepin retention rate is 85.1%, and the Hericium erinaceus polysaccharide retention rate is 85.7%; the functional liquid is added to the last 30 seconds of the steamed bun and flour process, and the total active retention rate of the finished product is 77.2%.

[0037] Example 2

[0038] The present embodiment is the preparation of Ganoderma lucidum and Lentinula edodes targeted active composite functional liquid, which comprises the following steps:

[0039] S1, take Ganoderma lucidum fruiting body 1000g, Lentinula edodes mycelium fermentation broth 3000g, mix and put into low temperature high shear ultrafine grinder, control temperature 30~40℃ throughout the process, carry out ultrafine grinding, get full component broken wall mixed slurry without obvious particle feeling; detected by particle size instrument, the average particle size of the mixed slurry is 3μm.

[0040] S2, replace the air in the tank with nitrogen gas with purity of more than 99.99%, transport the mixed slurry to the extraction tank under the protection of nitrogen, control the temperature at 32℃ and the negative pressure at-0.08MPa, continuously input nitrogen, add 6g microcrystalline vitamin C, and cycle extraction for 45min to obtain Ganoderma lucidum and Lentinula edodes crude extract; the detection of Ganoderma lucidum β-glucan retention rate is 94.5%, and the retention rate of Lentinula edodes purine components is 94.1%.

[0041] S3, the Ganoderma lucidum and Lentinula edodes crude extract is transported in the nitrogen sealed pipeline throughout the process, first filtered through 100~200 mesh filter cloth, then filtered through 0.2μm ceramic membrane, and clear extract is obtained; the extract is pre-cooled to 10℃, sent to high pressure pulse electric field sterilization, set the field strength to 25kV / cm, the pulse number to 12, and the pulse length to 200μs, and the temperature of the extract after sterilization is not more than 40℃.

[0042] S4, replace the oxygen content in the sterilized blending tank to 0.3% by blowing sterile nitrogen, transport the sterilized Ganoderma lucidum and Lentinula edodes extract into the blending tank under the protection of sterile nitrogen, and fill it into sterilized brown HDPE barrels. The detection of total bacterial count of the product is ≤10CFU / mL, the retention rate of Ganoderma lucidum β-glucan is 92.8%, and the retention rate of Lentinula edodes purine components is 92.5%.

[0043] After 18 months of storage at 25℃, the retention rate of Ganoderma lucidum β-glucan is 85.5%, and the retention rate of Lentinula edodes purine components is 85.1%; the functional liquid is added to the rice soaking process for the last 15 minutes, and the total retention rate of activity after cooking is 76.4%.

[0044] Example 3

[0045] The present embodiment is the preparation of Ganoderma lucidum and Lentinula edodes targeted active composite functional liquid, which comprises the following steps:

[0046] S1, 1000g of Cordyceps sinensis fruiting bodies, 200g of Ganoderma lucidum fruiting bodies, 5000g of Hericium erinaceus mycelium fermentation liquor and 7000g of Lentinula edodes mycelium fermentation liquor are mixed and then put into a low-temperature high-shear ultrafine grinder, and ultrafine grinding is carried out under the condition that the whole process is controlled at 30-40℃, to obtain a full-component broken-wall mixed slurry without obvious granular feeling; the average particle size of the mixed slurry is 4μm detected by a particle size instrument.

[0047] S2, nitrogen gas with a purity of more than 99.99% is introduced into the extraction tank to replace the air in the tank, and the mixed slurry is transported to the extraction tank under the protection of nitrogen gas, the temperature is controlled at 38℃, the negative pressure is-0.095MPa, nitrogen gas is continuously introduced, 33g of microcrystalline vitamin C is added, and after 30min of circulation extraction, a Cordyceps-Ganoderma-Lentinula-Hericium crude extract is obtained; the detection of cordycepin retention rate is 94.1%, the detection of Hericium erinaceus polysaccharide retention rate is 93.9%, the detection of Ganoderma lucidum β-glucan retention rate is 94.4%, and the detection of Lentinula edodes purine component retention rate is 94.0%.

[0048] S3, the Cordyceps-Ganoderma-Lentinula-Hericium crude extract is transported in a nitrogen gas sealed pipeline throughout the process, first filtered through 100-200 mesh filter cloth, and then filtered through 0.2μm ceramic membrane, to obtain a clear extract; the extract is pre-cooled to 15℃ and then sent to high-voltage pulse electric field sterilization, the field strength is set to 35kV / cm, the pulse number is 8, the pulse length is 350μs, and after sterilization, the temperature of the extract is not more than 40℃.

[0049] S4, sterile nitrogen gas is introduced into the sterilized blending tank to replace oxygen to 0.4%, and the sterilized extract is transported to the blending tank under the protection of sterile nitrogen gas, and then filled into sterilized brown HDPE barrels. The total number of colonies is ≤10CFU / mL, the retention rate of cordycepin is 92.4%, the retention rate of Hericium erinaceus polysaccharide is 92.5%, the retention rate of Ganoderma lucidum β-glucan is 92.7%, and the retention rate of Lentinula edodes purine component is 92.3%.

[0050] After 18 months of storage at 25℃, the cordycepin retention rate is 86.0%, the Hericium erinaceus polysaccharide retention rate is 85.8%, the Ganoderma lucidum β-glucan retention rate is 85.3%, and the Lentinula edodes purine component retention rate is 86.1%; the functional liquid is added to the yogurt preparation process, and the total activity retention rate of the finished product is 91.8%.

[0051] Example 4

[0052] The sterilized Cordyceps-Hericium extract prepared in Example 1 and the sterilized Ganoderma-Lentinula extract prepared in Example 2 are transported to the blending tank after oxygen replacement sterilization under the protection of sterile nitrogen gas, mixed uniformly, and then filled into sterilized brown HDPE barrels.

[0053] Comparative Example 1

[0054] The comparative example is the preparation of Cordyceps militaris and Hericium erinaceus targeted active composite functional liquid. The raw material dosage is the same as that of Example 1, and a conventional preparation method is adopted, including the following steps:

[0055] S1, take Cordyceps militaris fruiting body 1000g and Hericium erinaceus mycelium fermentation broth 5000g, mix and put into a high-shear ultrafine grinder for ultrafine grinding. The ultrafine grinding process is not temperature-controlled, and a full-component broken wall mixed slurry with no obvious particle feeling is obtained. The average particle size of the mixed slurry is 5μm.

[0056] S2, the mixed slurry is transported to a leaching tank, 12g of vitamin C is added, the temperature is controlled at 100℃, and after 5h of circulation leaching, Cordyceps militaris and Hericium erinaceus crude extract is obtained.

[0057] S3, the Cordyceps militaris and Hericium erinaceus crude extract is first coarsely filtered through 100-200 mesh filter cloth and then precisely filtered through 0.2μm ceramic membrane under air atmosphere to obtain a clear extract. The extract is subjected to 85℃ pasteurization (pasteurization temperature 85℃, pasteurization time 21min).

[0058] S4, the sterilized Cordyceps militaris and Hericium erinaceus extract is transported to a sterile blending tank under sterile air atmosphere and filled into a sterilized brown HDPE barrel. The total number of colonies of the product is detected to be 230CFU / mL, the retention rate of cordycepin is 58.4%, and the retention rate of Hericium erinaceus polysaccharide is 58.0%.

[0059] After 18 months of storage at 25℃, the retention rate of cordycepin is 32.5%, and the retention rate of Hericium erinaceus polysaccharide is 31.8%. The functional liquid is added to the last 30 seconds of the steamed bun and dough process, and the total active retention rate of the finished product is 18.3%.

[0060] Comparative Example 2

[0061] The difference from Comparative Example 1 is that S2 adopts low-temperature negative pressure leaching: the mixed slurry is transported to a leaching tank, the temperature is controlled at 35℃, and the negative pressure is-0.09MPa. After 40min of circulation leaching, Cordyceps militaris and Hericium erinaceus crude extract is obtained.

[0062] Comparative Example 3

[0063] The difference from Comparative Example 1 is that the leaching, transportation, sterilization and filling operations are all carried out under nitrogen protection.

[0064] Comparative Example 4

[0065] The difference from Comparative Example 1 is that the vitamin C is replaced by an equal amount of microcrystalline vitamin C.

[0066] Comparative Example 5

[0067] The difference from Example 1 is that no microcrystalline vitamin C is added.

[0068] Comparative Example 6

[0069] The difference from Example 1 is that no nitrogen protection measures are taken, and the whole operation is carried out in an air atmosphere.

[0070] Comparative Example 7

[0071] The difference from Example 1 is that S2 does not use low-temperature negative pressure extraction, but uses normal-pressure high-temperature extraction, and the extraction temperature is controlled at 100℃, and the normal-pressure extraction is carried out for 5h.

[0072] Comparative Example 8

[0073] The difference from Example 1 is that the extracted liquid after precision filtration does not undergo high-voltage pulse field sterilization, but is directly transported into a sterilized and oxygen-replaced blending tank under the protection of sterile nitrogen, and is bottled.

[0074] Comparative Example 9

[0075] The difference from Example 1 is that microcrystalline vitamin C is replaced with an equal amount of vitamin C.

[0076] Comparative Example 10

[0077] The difference from Example 1 is that the Hericium erinaceus mycelium fermentation broth is replaced with an equal amount of water; the detection of cordycepin retention rate in step S2 is 81.7%, and the detection of solid residues confirms that the reduction of retention rate is actually the reduction of extraction efficiency of cordycepin.

[0078] Comparative Example 11

[0079] The difference from Example 2 is that the Lentinula edodes mycelium fermentation broth is replaced with an equal amount of water; the detection of Ganoderma lucidum β-glucan retention rate in step S2 is 92.1%, and the detection of solid residues confirms that the reduction of retention rate is actually the reduction of extraction efficiency of Ganoderma lucidum β-glucan.

[0080] The detection methods and standards of the content of each active ingredient and the total number of miscellaneous bacteria are as follows:

[0081] Cordycepin: HPLC method, referring to the Cordyceps item in Chinese Pharmacopoeia 2020 edition part one;

[0082] Hericium erinaceus polysaccharide: phenol-sulfuric acid method, referring to GB 5009.8-2016;

[0083] Ganoderma lucidum β-glucan: enzyme-linked immunosorbent assay, referring to NY / T 1676-2008;

[0084] Lentinan: ultra-performance liquid chromatography-tandem mass spectrometry method, referring to QB / T 5525-2020;

[0085] Total number of miscellaneous bacteria: plate count method, referring to GB 4789.2-2022.

[0086] The products of Example 1 and Comparative Examples 1 to 9 were respectively detected for cordycepin and hericium erinaceus polysaccharide content and total number of miscellaneous bacteria, and the total active ingredient retention rate was calculated from the cordycepin and hericium erinaceus polysaccharide content, and the detection results are shown in Table 1:

[0087] Table 1

[0088]

[0089] The total active ingredient retention rate is the average of the retention rates of each active ingredient.

[0090] The application has been described in detail according to the above examples. It should be noted that the above examples are only for the purpose of illustrating the application. Those skilled in the art can design various alternative schemes and improvements of the application without departing from the spirit and essence of the application, which should be understood as being within the scope of protection of the application.

Claims

1. A method for preparing a high-retention-rate targeted active edible fungus compound functional liquid with end-to-end activation, characterized in that, Includes the following steps: S1. Mix the fruiting bodies of edible fungi and the fermentation broth of fungal mycelium, and then pulverize them into ultrafine particles until the cell walls are broken to obtain a mixed slurry. S2. Add microcrystalline vitamin C to the mixed slurry obtained in step S1, and perform low-temperature negative pressure extraction under nitrogen protection to obtain crude extract; S3. The crude extract obtained in step S2 is subjected to coarse filtration and fine filtration under nitrogen protection to obtain a clear extract; the extract is then placed in a high-voltage pulsed electric field for sterilization. S4. The sterilized liquid from step S3 is aseptically filled under nitrogen positive pressure protection.

2. The method for preparing a high-retention-rate targeted active edible fungus compound functional liquid with end-to-end activation according to claim 1, characterized in that, In step S1, the mass ratio of edible fungal fruiting bodies to fungal mycelium fermentation broth is 1:(3~10); the edible fungal fruiting bodies are selected from Cordyceps sinensis fruiting bodies and / or Ganoderma lucidum fruiting bodies; the fungal mycelium fermentation broth is selected from Hericium erinaceus mycelium fermentation broth and / or Lentinus edodes mycelium fermentation broth.

3. The method for preparing a high-retention-rate targeted active edible fungus compound functional liquid with end-to-end activation lock according to claim 2, characterized in that, The temperature of the ultrafine grinding process in step S1 is controlled at 30~40℃, and the average particle size of the mixed slurry obtained after ultrafine grinding is ≤5μm.

4. The method for preparing a high-retention-rate targeted active edible fungus compound functional liquid with end-to-end activation according to claim 1, characterized in that, The extraction temperature in step S2 is 32~38℃, the pressure is -0.08~-0.095MPa, and the extraction time is 30~45min.

5. The method for preparing a high-retention-rate targeted active edible fungus compound functional liquid with end-to-end activation according to claim 4, characterized in that, In step S2, the amount of microcrystalline vitamin C added is 0.15% to 0.25% of the total mass of the extraction system; the particle size of the microcrystalline vitamin C is 200 to 300 nm.

6. The method for preparing a high-retention-rate targeted active edible fungus compound functional liquid with end-to-end activation according to claim 1, characterized in that, Step S3 involves coarse filtration using a 100-200 mesh filter cloth and fine filtration using a 0.2μm ceramic membrane.

7. A method for preparing a high-retention-rate targeted active edible fungus compound functional liquid with end-to-end activation according to any one of claims 1 to 6, characterized in that, The parameters for high-voltage pulsed electric field sterilization are: electric field strength 25~35kV / cm, number of pulses 8~12, and processing temperature not higher than 40℃.

8. The method for preparing a high-retention-rate targeted active edible fungus compound functional liquid with end-to-end activation according to claim 7, characterized in that, In step S4, the oxygen content in the liquid-material contact space is ≤0.5% throughout the entire process.

9. A high-retention-rate targeted active edible fungi compound functional liquid, characterized in that, The high-retention-rate targeted active edible fungus compound functional liquid is prepared by the full-chain activation locking method according to any one of claims 1 to 8. The high-retention-rate targeted active edible fungus compound functional liquid is stored in the dark at room temperature, and the active ingredient retention rate is ≥85% after 18 months of storage.

10. The application of the high-retention-rate targeted active edible fungi compound functional liquid according to claim 9 in civilian staple foods or low-temperature foods.

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