A pistachio-containing composition, a method of making the same, and use in improving mood and sleep

Abstract

The application discloses a pistachio-containing composition and a preparation method and application thereof in improving mood and sleep, and relates to the technical field of functional food and health products.The pistachio-containing composition comprises the following components in mass fractions: 50-150 parts of pistachio extract, 20-80 parts of lemongrass extract, 20-80 parts of aronia melanocarpa fruit extract, 30-70 parts of perilla leaf extract and 10-40 parts of banana flower extract.The raw material components of the composition are edible extracts, have high safety, no toxic side effects, are easy to be absorbed and utilized by human bodies, and can improve sleep quality and mood through the synergistic effect of multiple levels, multiple targets and multiple angles.

Description

Technical Field

[0001] This invention relates to the field of functional foods and health products, and in particular to a pistachio-containing composition, its preparation method, and its application in improving mood and sleep. Background Technology

[0002] With the continued acceleration of the pace of social life and the increase in psychosocial stressors, neuropsychiatric dysfunction, characterized by mood disorders (anxiety, depression) and sleep disorders (difficulty falling asleep, difficulty maintaining sleep, early awakening), has become a significant problem seriously affecting public health. Patients with depression often experience insomnia, and those with chronic insomnia have a several times higher risk of developing mood disorders than the general population. Long-term comorbidity of mood and sleep not only severely reduces quality of life but also, by activating the sympathetic-adrenal medullary system and the hypothalamic-pituitary-adrenal (HPA) axis, causes a sustained increase in glucocorticoid levels, thereby impairing hippocampal neuroplasticity, accelerating neurodegenerative processes, and is closely related to the development of physical diseases such as hypertension, type 2 diabetes, and immunosuppression.

[0003] From a neurobiological perspective, the regulation of the sleep-wake cycle depends on the coordinated operation of monoaminergic neuronal groups such as the suprachiasmatic nucleus of the hypothalamus, the ascending reticular formation in the brainstem, and the locus coeruleus and raphe nuclei. The balance between GABAergic inhibition and glutamatergic excitation is crucial for sleep initiation and maintenance. Emotional regulation, centered on the prefrontal cortex-amygdala-hippocampus circuit, is finely regulated by monoamine neurotransmitters such as serotonin, norepinephrine, and dopamine. Under chronic stress, immune cells in the central nervous system can be activated, promoting the release of pro-inflammatory factors such as tumor necrosis factor-α and interleukin-6 through the NF-κB signaling pathway and the NLRP3 inflammasome. These inflammatory mediators can interfere with the tryptophan-kynurenine metabolic pathway, reducing serotonin synthesis and affecting the expression of brain-derived neurotrophic factor, thereby impairing synaptic plasticity.

[0004] Currently, clinical interventions for these problems mainly rely on chemically synthesized drugs. Sedative-hypnotics (such as benzodiazepines and Z-drugs) exert rapid hypnotic effects through positive allosteric regulation of GABA-A receptors, but long-term use can easily lead to drug tolerance, cognitive impairment, abnormal proportion of slow-wave sleep during the NREM period, and severe rebound insomnia after withdrawal. Selective serotonin reuptake inhibitors can increase serotonin levels in the synaptic cleft to alleviate depression and anxiety, but their onset of action is delayed, their improvement on sleep continuity is limited, and some drugs can induce or aggravate insomnia and daytime sleepiness. Melatonin receptor agonists and orexin receptor antagonists can regulate circadian rhythms or reduce excessive arousal to some extent, but their single-target action mode cannot cover the multiple pathophysiological links of mood-sleep comorbidities, and there are problems of large individual variability and unstable efficacy. More importantly, existing drugs mostly focus on a single neurotransmitter system and lack the ability to intervene in the overall way of upstream neuroinflammation, oxidative stress, and neuroplasticity damage, making it difficult to achieve the dual repair of mood improvement and sleep structure.

[0005] Therefore, developing a natural source composition that combines the functions of regulating neurotransmitters, anti-inflammation, anti-oxidation, and regulating sleep rhythm, while simultaneously improving mood and sleep, has significant application prospects and market value. Summary of the Invention

[0006] The main objective of this invention is to propose a pistachio-containing composition and its preparation method, as well as its application in improving mood and sleep, aiming to solve the problems of existing sleep aids and anti-anxiety products having single effects, limited efficacy, strong dependence, or side effects.

[0007] To achieve the above objectives, the present invention provides a pistachio-containing composition, which comprises the following components in parts by weight: 50-150 parts pistachio extract, 20-80 parts lemon daylily extract, 20-80 parts black chokeberry fruit extract, 30-70 parts perilla leaf extract, and 10-40 parts banana flower extract.

[0008] In one embodiment, the pistachio-containing composition comprises the following components in parts by weight: 80-120 parts pistachio extract, 40-60 parts lemon daylily extract, 40-60 parts black chokeberry fruit extract, 40-60 parts perilla leaf extract, and 20-30 parts banana flower extract.

[0009] In one embodiment, the preparation method of the pistachio extract includes the following steps: taking pistachio kernels, adding 6-10 times their weight of water, ultrasonically extracting, concentrating, and freeze-drying to obtain pistachio extract.

[0010] In one embodiment, the preparation method of the lemon daylily extract includes the following steps: take dried lemon daylily flowers, add 8-12 times the weight of water, decoct and extract, concentrate, and dry to obtain lemon daylily extract.

[0011] In one embodiment, the preparation method of the black chokeberry fruit extract includes the following steps: taking dried black chokeberry fruit, adding water at a material-to-liquid ratio of 1:30-40, ultrasonically extracting, concentrating, and drying to obtain the black chokeberry fruit extract.

[0012] In one embodiment, the preparation method of the perilla leaf extract includes the following steps: take dried perilla leaves, add water at a material-to-liquid ratio of 1:30-40, extract at 85-95℃ for 40-60 minutes, extract 1-3 times, concentrate, and dry to obtain perilla extract.

[0013] In one embodiment, the preparation method of the banana flower extract includes the following steps: taking dried banana flowers, adding water at a material-to-liquid ratio of 1:20-40, extracting by ultrasonication at 40-50℃, concentrating, and drying to obtain the banana flower extract.

[0014] The present invention also provides a method for a pistachio-containing composition, comprising the following steps: mixing pistachio extract, lemon daylily extract, black chokeberry fruit extract, perilla leaf extract and banana flower extract, adding a wetting agent, granulating, sieving, and drying to obtain a pistachio-containing composition; wherein the wetting agent is selected from at least one of pure water and edible alcohol.

[0015] The present invention also provides an application of the pistachio-containing composition in the preparation of functional foods or health foods that improve mood.

[0016] The present invention also provides an application of the pistachio-containing composition in the preparation of functional foods or health foods that improve sleep quality.

[0017] The pistachio-containing composition of this invention includes pistachio extract, lemon daylily extract, black chokeberry fruit extract, perilla leaf extract, and banana flower extract. All extracts are of edible origin and are characterized by high safety. When used in combination, the five components achieve full-chain coverage of the anti-inflammatory pathway from occurrence and transduction to effect. Antioxidants are complementary, and neurotransmitters and neurotrophic factors are enhanced through multiple pathways. Through multi-level, multi-target, and multi-angle synergistic effects, the composition aims to improve sleep quality and mood. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially. Furthermore, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, or solution B, or a solution where both A and B are satisfied simultaneously. In addition, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] With the fast pace of modern life, work and life pressures are leading to increasingly prominent emotional and sleep disorders such as anxiety, depression, and insomnia. Prolonged negative emotions not only affect quality of life but can also trigger a series of physiological health problems. While various sleep aids and anti-anxiety products are currently available on the market, most suffer from issues such as single-ingredient formulations, limited effectiveness, strong dependence, and side effects. For example, chemically synthesized drugs are prone to tolerance and dependence; melatonin-based products only regulate sleep rhythms and have limited effects on mood regulation. Therefore, developing a safe and effective natural-source composition that combines both mood regulation and sleep improvement has significant market value.

[0020] In view of this, the present invention provides a pistachio-containing composition, wherein the pistachio-containing composition comprises the following components in parts by weight: 50-150 parts of pistachio extract, 20-80 parts of lemon daylily extract, 20-80 parts of black chokeberry fruit extract, 30-70 parts of perilla leaf extract, and 10-40 parts of banana flower extract.

[0021] In the technical solution of this invention, pistachio extract can block inflammatory signals at the source by inhibiting NF-κB and NLRP3 inflammasomes, and is also rich in melatonin and hydroxy acids, thus having rapid antioxidant effects and direct regulation of sleep rhythm; lemon daylily extract can inhibit indoleamine 2,3-dioxygenase activity, increase serotonin synthesis, and promote brain-derived neurotrophic factor expression, directly acting on the neurotransmitter synthesis link; black chokeberry fruit extract can inhibit the production of pro-inflammatory cytokines interleukin-6 and tumor necrosis factor-α at the transcriptional level, and provide long-term free radical scavenging protection, acting on the inflammatory effect stage; perilla leaf extract is rich in rosmarinic acid, which can exert neuroprotective effects, inhibit neuroinflammation and reduce oxidative stress damage, maintain normal neuronal function, and thus synergistically improve mood and sleep; banana flower extract contains stamen polysaccharides, which can exert antioxidant effects, scavenge free radicals, reduce systemic oxidative stress levels, and provide a protective microenvironment for the nervous system. When the five components are used in combination, the anti-inflammatory pathway is fully covered from occurrence and transduction to effect. Antioxidants are complementary, and neurotransmitters and neurotrophic agents are enhanced through multiple pathways. Through multi-level, multi-target, and multi-angle synergistic effects, the goal of improving sleep quality and mood can be achieved.

[0022] The pistachio-containing composition of the present invention comprises the following components in parts by weight: 80-120 parts pistachio extract, 40-60 parts lemon daylily extract, 40-60 parts black chokeberry fruit extract, 40-60 parts perilla leaf extract, and 20-30 parts banana flower extract.

[0023] Understandably, the optimal proportions of pistachio extract, lemon daylily extract, black chokeberry fruit extract, perilla leaf extract, and banana flower extract are 80-120 parts, 40-60 parts, 40-60 parts, 40-60 parts, and 20-30 parts, respectively, to achieve the best balance between efficacy, cost, and process.

[0024] In some embodiments of the present invention, the preparation method of the pistachio extract includes the following steps: taking pistachio kernels, adding 6-10 times their weight of water, ultrasonically extracting, concentrating, and freeze-drying to obtain pistachio extract.

[0025] Understandably, pistachio kernels, after water extraction, ultrasonication, concentration, and freeze-drying, can yield an extract containing hydroxy acids and their derivatives. This process effectively disrupts cell walls, improves extraction efficiency, and preserves the activity of heat-sensitive components at low temperatures.

[0026] Pistachio kernels and seed coats contain hydroxy acids and their derivatives. The hydroxyl groups in their molecular structure can efficiently scavenge various reactive oxygen species (ROS) through hydrogen atom transfer mechanisms (such as superoxide anions, hydrogen peroxide, and hydroxyl radicals). At the same time, their ability to chelate metal ions can reduce the production of ROS signaling molecules from the source of inflammation initiation, inhibit the activation of the NF-κB signaling pathway and the activation of the NLRP3 inflammasome, thereby alleviating astrocyte inflammation and exhibiting neuroprotective effects on MPTP-induced Parkinson's disease model mice.

[0027] In some embodiments of the present invention, the preparation method of the lemon daylily extract includes the following steps: taking dried lemon daylily flowers, adding 8-12 times the weight of water, decocting and extracting, concentrating, and drying to obtain lemon daylily extract.

[0028] Understandably, water extraction is used to separate and enrich specific active ingredients. Boiling the plant in water at high temperatures breaks down the plant cell walls, allowing water-soluble components (such as flavonoids, phenolic acids, and polysaccharides) to fully dissolve. The extract is then concentrated and dried to obtain the final product.

[0029] Lemon daylily, also known as golden chrysanthemum, is traditionally believed in for its calming and mood-lifting effects. Modern research confirms that its coumaroyl quinic acid and its isomers, such as 3-O-p-coumaroyl quinic acid, 4-O-p-coumaroyl quinic acid, and 5-O-p-coumaroyl quinic acid, are key substances responsible for its activity. Studies have also shown that lemon daylily extract can increase the levels of serotonin, dopamine, and norepinephrine in the cerebral cortex and hippocampus of mice with chronic unpredictable stress. It is speculated that this mechanism may be related to regulating the activity of indoleamine 2,3-dioxygenase to balance the tryptophan-kynurenine metabolic pathway, thereby inhibiting kynurenine production by positively regulating the tryptophan metabolic pathway and thus helping to maintain central serotonin homeostasis. Furthermore, research indicates that lemon daylily extract can significantly increase the levels of brain-derived neurotrophic factor (BDNF) in the cerebral cortex and hippocampus of mice with chronic unpredictable stress.

[0030] In some embodiments of the present invention, the preparation method of the black chokeberry fruit extract includes the following steps: taking dried black chokeberry fruit, adding water at a material-to-liquid ratio of 1:30-40, ultrasonically extracting, concentrating, and drying to obtain black chokeberry fruit extract.

[0031] Understandably, a material-to-liquid ratio of 1:30-40 provides sufficient solvent volume to ensure the full dissolution of active ingredients. Ultrasonic extraction utilizes the cavitation effect of ultrasound to disrupt plant cell walls, accelerating the release of intracellular active ingredients. At the same time, ultrasonic extraction is characterized by its short extraction time and lack of the need for high temperatures, effectively protecting heat-sensitive components such as anthocyanins from damage, thereby obtaining highly active extracts.

[0032] Chokeberry (Acer rubrum), also known as wild chokeberry, is a novel food ingredient. Its fruit is rich in polyphenolic compounds and is hailed as one of the most potent natural antioxidants capable of scavenging free radicals. Key active ingredients in its extract include chlorogenic acid, cyanidin-3-galactoside, and other anthocyanins, as well as proanthocyanidins of varying degrees of polymerization. Studies have shown that the polyphenols in Chokeberry can directly protect the brain and nervous system across the blood-brain barrier. Its anthocyanins and chlorogenic acid can inhibit the production of pro-inflammatory cytokines interleukin-6 and tumor necrosis factor-α at the transcriptional level, while proanthocyanidins possess highly efficient and long-lasting free radical scavenging capabilities.

[0033] In some embodiments of the present invention, the preparation method of the perilla leaf extract includes the following steps: take dried perilla leaves, add water at a material-to-liquid ratio of 1:30-40, extract at 85-95℃ for 40-60 minutes, extract 1-3 times, concentrate, and dry to obtain perilla extract.

[0034] Understandably, a liquid-to-solid ratio of 1:30-40 provides sufficient solvent volume to ensure the full dissolution of active ingredients; the gentle boiling state can accelerate molecular motion to extract active ingredients while avoiding the dissolution of excessive impurities such as chlorophyll.

[0035] Perilla leaves, the dried leaves of the Perilla frutescens plant (family Lamiaceae), are one of the plants used for both food and medicine. Perilla leaf extract is rich in various active ingredients, including rosmarinic acid, flavonoids, and volatile oils. Rosmarinic acid, a water-soluble natural phenolic acid compound, possesses strong antioxidant activity, effectively scavenging free radicals and preventing cell damage caused by them. Rosmarinic acid has interventional effects on various neurological disorders, improving learning and memory impairments and protecting nerve cells. Studies have shown that perilla leaf alcohol extract can increase superoxide dismutase and glutathione peroxidase levels, decrease malondialdehyde levels, and alleviate oxidative stress damage to nerve cells. Furthermore, rosmarinic acid in perilla leaves can promote myelin regeneration and improve cognitive dysfunction after brain injury. Through its multiple effects of antioxidation, anti-inflammation, and neuroprotection, perilla leaves support improved mood and sleep.

[0036] In some embodiments of the present invention, the preparation method of the banana flower extract includes the following steps: taking dried banana flowers, adding water at a material-to-liquid ratio of 1:20-40, extracting by ultrasonication at 40-50°C, concentrating, and drying to obtain banana flower extract.

[0037] It is understandable that adding water at a material-to-liquid ratio of 1:20-40 can create a sufficient concentration gradient, which is beneficial for the extraction of effective components from banana flowers. Combined with ultrasonic technology, it can prevent heat-sensitive components from being destroyed.

[0038] Banana flowers are a byproduct of banana harvesting and are rich in polysaccharides. Studies have shown that banana flower polysaccharides possess strong antioxidant activity, exhibiting excellent scavenging abilities against DPPH free radicals, hydroxyl radicals, and superoxide anion radicals. The total sugar content of banana flower polysaccharides can reach 515.61 mg / g, and they also contain uronic acid, protein, and small amounts of polyphenols and flavonoids. Banana flower polysaccharides can reduce systemic oxidative stress levels by scavenging free radicals, providing a protective microenvironment for the nervous system.

[0039] The present invention also provides a method for preparing a pistachio-containing composition, comprising the following steps: mixing pistachio extract, lemon daylily extract, black chokeberry fruit extract, perilla leaf extract and banana flower extract, adding a humectant, granulating, sieving, and drying to obtain a pistachio-containing composition.

[0040] Understandably, when pistachio extract, lemon daylily extract, black chokeberry fruit extract, perilla leaf extract, and banana flower extract are mixed, the humectant allows the powders with different physical properties to be uniformly bound together in a moist state, forming granules with consistent component distribution, avoiding stratification, and ensuring the accurate proportion of active substances in each dosage unit. Granulation can significantly improve the undesirable physical properties of extract powders, such as excessive fine powder, poor flowability, and easy hygroscopicity, giving them good flowability and compressibility, facilitating subsequent tableting or capsule filling, while reducing dust. After the mixture is made into granules, the surface area of ​​the granules is greatly reduced compared to the powder, reducing the contact opportunities between active ingredients and air and moisture, helping to slow down the degradation of easily oxidized components such as anthocyanins in black chokeberry fruit, improving product stability, and extending shelf life.

[0041] In some embodiments of the present invention, the wetting agent is selected from at least one of pure water and edible alcohol.

[0042] It is understandable that water or edible alcohol is chosen as a wetting agent when preparing pistachio-containing compositions, mainly based on a balance between safety, cost, process efficiency, and component stability. Water has the advantages of being non-toxic, low-cost, and widely applicable, making it suitable for most extract systems dominated by water-soluble components. Alcohol has lower surface tension, more uniform wetting, and faster evaporation. Both are recognized as safe solvents in the food industry.

[0043] The pistachio-containing composition of the present invention can be used in the preparation of functional foods or health foods that improve mood.

[0044] The pistachio-containing composition of the present invention can be used in the preparation of functional foods or health foods that improve sleep quality.

[0045] In some embodiments of the present invention, excipients are added to the pistachio-containing composition to prepare tablets, oral liquids, capsules, granules, powders or pills to obtain functional foods or health foods that improve mood or improve sleep quality.

[0046] The technical solution of the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are only used to explain the present invention and are not intended to limit the present invention.

[0047] The method for preparing the extract used in this invention is as follows: Preparation method of pistachio extract: Take pistachio kernels, add 8 times their weight of water, extract by ultrasonication, concentrate, freeze dry to obtain pistachio extract.

[0048] Preparation method of lemon daylily extract: Take dried lemon daylily flowers, add 10 times their weight of water, decoct and extract, concentrate, and dry to obtain lemon daylily extract.

[0049] Preparation method of black chokeberry fruit extract: Take dried black chokeberry fruit, add water at a material-to-liquid ratio of 1:35, extract by ultrasonication, concentrate, and dry to obtain black chokeberry fruit extract.

[0050] Preparation method of perilla leaf extract: Take dried perilla leaves, add water at a material-to-liquid ratio of 1:35, extract at 90℃ for 50 minutes, extract 3 times, concentrate, and dry to obtain perilla extract.

[0051] Preparation method of banana flower extract: Take dried banana flowers, add water at a material-to-liquid ratio of 1:25, extract by ultrasonication at 45℃, concentrate, and dry to obtain banana flower extract.

[0052] The preparation method of the compositions of Examples 1-3 and Comparative Examples 1-4 of the present invention is as follows: according to the formula parts in Table 1 (all values ​​in the table are parts), add 15% water by total mass, mix evenly, granulate, sieve, and dry to obtain the final product.

[0053] Table 1

[0054] Performance testing 1. Sleep experiment on mice Healthy SPF-grade male mice aged 6-8 weeks and weighing 18-22g were selected and housed at a temperature of (24±2)℃ and a relative humidity of 39%-69%. They were divided into 9 groups (n=20 per group, with 10 mice per group participating in experiments 1.2 and 1.3 respectively): blank control group (distilled water), positive control group (melatonin), Examples 1-3 groups, and Comparative Examples 1-4 groups. The melatonin dosage was 1 mg / kg, and the dosage for all other groups was 200 mg / kg.

[0055] 1.1 Direct Sleep Experiment: Nine groups of mice were administered different doses of the test sample at 9:00 AM daily for 30 consecutive days, with a gavage volume of 0.2 mL / 20 g. After the last gavage, the presence of sleep was observed in each group of mice. Sleep was defined as the disappearance of the righting reflex (when a normal mouse is in a supine position, it will immediately turn over to its right position). If the mouse could not turn over for more than 60 seconds, the righting reflex was considered to have disappeared, indicating sleep; the recovery of the righting reflex indicated awakening. The results showed that no mice in any group experienced sleep at the administered doses. This indicates that there was no direct sleep effect in any of the experimental groups.

[0056] 1.2 Experiment on prolonging pentobarbital sodium sleep time: Nine groups of mice were given different doses of the test sample at 9:00 AM daily, with a gavage volume of 0.2 mL / 20 g, for 30 consecutive days. Thirty minutes after the last gavage, each group of animals was intraperitoneally injected with 45 mg / kg pentobarbital sodium at a dose of 0.2 mL / 20 g. The disappearance of the righting reflex was used as an indicator to observe whether the test sample could prolong pentobarbital sodium sleep time. The results are shown in Table 2.

[0057] 1.3 Sodium barbital sleep latency experiment: Nine groups of mice were given different doses of the test sample at 9:00 AM daily, with a gavage volume of 0.2 mL / 20 g, for 30 consecutive days. Thirty minutes after the last gavage, each group of animals was intraperitoneally injected with 260 mg / kg sodium barbital at a dose of 0.2 mL / 20 g. The effect of the test sample on the sodium barbital sleep latency was observed using the disappearance of the righting reflex as an indicator. The results are shown in Table 2.

[0058] Table 2

[0059] (Compared with the blank control group, The result is significant, P < 0.05; (Indicates highly significant, P < 0.01) The mice in Example 2 had significantly longer sleep duration than the control group (P < 0.01) and significantly shorter sleep onset time than the control group (P < 0.01), and the sleep-aiding effect was better than that in Comparative Examples 1-4, indicating that the composition of the present invention has a significant sleep-improving effect.

[0060] 2. Effects on mouse mood experiments Healthy male SPF mice aged 6-8 weeks and weighing 18-22g were selected and housed at a temperature of (24±2)℃ and a relative humidity of 39%-69%. After one week of acclimatization, 10 mice were randomly selected as the normal control group, while the other mice were housed in solitary cages and subjected to depression modeling.

[0061] 2.1 Except for 10 normal control mice, the remaining mice were subjected to chronic unpredictable stress modeling, including fasting and water restriction (24h), 4℃ ice water bath (5min), foot shock (1min, 2mA), noise (6h), tail clamping (2min), wet cage (150mL water added to bedding, 24h), day-night reversal, and restraint (12h) (mice were placed in 50mL centrifuge tubes with 4-5 0.5mm diameter vents in the tube wall). During restraint, the animals were fasted and water restricted. Chronic unpredictable stress modeling was performed weekly. The modeling was carried out for 42 consecutive days, and the success of the modeling was determined by the sucrose preference test. The successfully modeled mice were randomly divided into 9 groups of 10 mice each: model group (distilled water), positive control group (fluoxetine hydrochloride), experimental groups 1-3, and control groups 1-4. The dose of fluoxetine hydrochloride was 5.2mg / kg, and the dose of other groups was 200mg / kg. The normal control group and the model group were administered 0.4 mL of distilled water by gavage daily, while the other groups were administered the corresponding drug solution in 0.4 mL volumes for 35 consecutive days. After each administration, the mice were subjected to chronic unpredictable stress. Finally, after the last administration, behavioral tests such as the tail suspension test and forced swimming test were performed on the mice.

[0062] 2.2 Tail Suspension Experiment The tail suspension test is often used to detect behavioral hopelessness in depressive symptoms. Mice were inverted and secured to a homemade tail suspension device 2 cm from the base of their tails with transparent tape. The activity of each mouse was observed for 6 minutes after tail suspension, and the cumulative immobility time for the following 4 minutes was recorded (the experimental environment was kept quiet and undisturbed during the test). The results are shown in Table 3.

[0063] 2.3 Forced Swimming Experiment The forced swimming test is also a commonly used method for detecting behavioral hopelessness in depressive symptoms. The forced swimming apparatus is a cylindrical glass container, 45 cm high and 20 cm in diameter. During the test, water is generally added to 2 / 3 of the container, and the water temperature is maintained at around 25°C. After placing the mouse in the water, its swimming performance is observed during the 5-minute swimming period, and the cumulative immobility time in the last 4 minutes is recorded (immobility is defined as the mouse ceasing to struggle or only exhibiting slight forelimb movements). The results are shown in Table 3.

[0064] Table 3

[0065] (Compared to the model group, The result is significant, P < 0.05; (Indicates highly significant, P < 0.01) The resting time of the mice in Example 2 was significantly shorter than that in the model group (P < 0.01), and the antidepressant effect was better than that in Comparative Examples 1-4, indicating that the composition of the present invention has significant antidepressant and mood-improving effects.

[0066] In summary, the sleep-aiding and antidepressant effects of mice in Example 2 were significantly better than those in other examples and comparative examples. This may be because the anti-inflammatory pathway achieved full-chain coverage from upstream signal initiation, midstream neurotransmitter synthesis to downstream inflammatory effects. Antioxidants formed a complementary combination of rapid clearance, intermediate maintenance and long-term protection. Neurotransmitters and neurotrophic and neuroprotective effects were enhanced through multiple pathways, thus resulting in significant sleep-aiding and antidepressant effects in mice.

[0067] The above are merely preferred embodiments of the present invention and do not limit the patent scope of the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the patent protection scope of the present invention.

Claims

1. A composition containing pistachios, characterized in that, The pistachio-containing composition comprises the following components in parts by weight: 50-150 parts pistachio extract, 20-80 parts lemon daylily extract, 20-80 parts black chokeberry fruit extract, 30-70 parts perilla leaf extract, and 10-40 parts banana flower extract.

2. The pistachio-containing composition as described in claim 1, characterized in that, The pistachio-containing composition comprises the following components in parts by weight: 80-120 parts pistachio extract, 40-60 parts lemon daylily extract, 40-60 parts black chokeberry fruit extract, 40-60 parts perilla leaf extract, and 20-30 parts banana flower extract.

3. The pistachio-containing composition as described in claim 1 or 2, characterized in that, The preparation method of the pistachio extract includes the following steps: take pistachio kernels, add 6-10 times their weight of water, extract by ultrasonication, concentrate, freeze dry, and obtain pistachio extract.

4. The pistachio-containing composition as described in claim 1 or 2, characterized in that, The preparation method of the lemon daylily extract includes the following steps: take dried lemon daylily flowers, add 8-12 times the weight of water, decoct and extract, concentrate, and dry to obtain lemon daylily extract.

5. The pistachio-containing composition as described in claim 1 or 2, characterized in that, The preparation method of the black chokeberry fruit extract includes the following steps: take dried black chokeberry fruit, add water at a material-to-liquid ratio of 1:30-40, extract by ultrasonication, concentrate, and dry to obtain black chokeberry fruit extract.

6. The pistachio-containing composition as described in claim 1 or 2, characterized in that, The preparation method of the perilla leaf extract includes the following steps: take dried perilla leaves, add water at a material-to-liquid ratio of 1:30-40, extract at 85-95℃ for 40-60 minutes, extract 1-3 times, concentrate, and dry to obtain perilla extract.

7. The pistachio-containing composition as described in claim 1 or 2, characterized in that, The preparation method of the banana flower extract includes the following steps: take dried banana flowers, add water at a material-to-liquid ratio of 1:20-40, extract by ultrasonication at 40-50℃, concentrate, and dry to obtain banana flower extract.

8. A method for preparing a pistachio-containing composition as described in any one of claims 1-7, characterized in that, The process includes the following steps: mixing pistachio extract, lemon daylily extract, black chokeberry fruit extract, perilla leaf extract, and banana flower extract, adding a humectant, granulating, sieving, and drying to obtain a pistachio-containing composition; wherein the humectant is selected from at least one of pure water and edible alcohol.

9. The use of a pistachio-containing composition as described in any one of claims 1-7 or a pistachio-containing composition prepared by the preparation method of the pistachio-containing composition as described in claim 8 in the preparation of functional foods or health foods that improve mood.

10. The use of a pistachio-containing composition as described in any one of claims 1-7 or a pistachio-containing composition prepared by the preparation method of the pistachio-containing composition as described in claim 8 in the preparation of functional foods or health foods that improve sleep quality.

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