A beverage based on composite functional peptide synergistic targeting repair and a preparation method thereof

Abstract

The application discloses a beverage based on composite functional peptide synergistic targeted repair and a preparation method thereof. The beverage comprises the following components: a composite functional peptide composition treated by nano-liposome embedding, a natural adaptogen, a cell energy activator, a foam stabilizer, a sweetening agent, an acidifying agent and pure water, and is filled with carbon dioxide gas. The composite functional peptide composition is composed of a neuroprotective peptide, a metabolic scavenging peptide and an anti-oxidation immune peptide. The application adopts a nano-embedding technology to isolate the polypeptide and the carbonic acid base liquid at a physical level, eliminates the foam spouting phenomenon caused by the surface activity of the polypeptide, blocks the hydrolytic degradation of the polypeptide in an acidic environment and hides the peculiar smell of the peptide. A three-step gradient aeration and low-temperature mixing process is adopted in the preparation process. The product plays a role by accelerating metabolic waste scavenging and adjusting neurotransmitters, eliminates the side effects of traditional caffeinated beverages, has the advantages of high physical stability and good sensory experience.

Description

Technical Field

[0001] This invention relates to the field of functional beverage manufacturing technology, specifically to a beverage based on the synergistic targeted repair of complex functional peptides and its preparation method. Background Technology

[0002] Functional beverages are drinks that adjust the composition and proportion of natural nutrients to meet the nutritional needs of specific groups. In modern work and lifestyles, frequent late nights and shift work lead to fatigue and abnormal metabolic indicators. Anti-fatigue functional beverages, which replenish energy and relieve physical fatigue, are widely used in the beverage market.

[0003] Current anti-fatigue functional beverages are mainly carbonated or water-based, and typically rely on added caffeine, taurine, and high sugar content as functional ingredients. After consumption, they primarily work by blocking fatigue signals in the brain through central nervous system stimulants, maintaining alertness for a short period. Some beverages also contain plant extracts or essential vitamins to maintain basic energy metabolism needs.

[0004] With increasing health demands, some studies are attempting to incorporate physiologically active peptides into beverages as an alternative to traditional central nervous system stimulants. However, direct application of peptides to carbonated beverage systems presents physical and chemical compatibility challenges. Peptide molecules possess an amphiphilic structure, altering the interfacial properties of carbonated beverages and reducing surface tension. This causes carbon dioxide gas to expand and escape when the beverage is opened and depressurized, resulting in foaming. Furthermore, the acidic environment of carbonated beverages easily leads to conformational changes and hydrolytic failure of peptides, and some peptides impart a fishy or astringent odor. Current technologies struggle to achieve stable addition of peptides while maintaining the carbonated state and sensory quality of carbonated beverages. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a beverage based on the synergistic targeted repair of complex functional peptides and its preparation method. This solves the problem that existing anti-fatigue beverages often rely on central nervous system stimulants such as caffeine, which mask fatigue signals by blocking adenosine receptors and fail to clear metabolic waste and repair cell damage.

[0006] To address the above problems, the present invention provides the following technical solution: In a first aspect, the present invention provides a beverage based on the synergistic targeted repair of complex functional peptides, employing the following technical solution: A beverage based on the synergistic targeted repair of complex functional peptides comprises the following components in parts by weight: 0.20–0.60 parts of a complex functional peptide composition encapsulated in nanoliposomes; 0.01–0.08 parts of a natural adaptogen; 0.001–0.13 parts of a cell energy activator; 0.05–0.15 parts of a foam stabilizer; 0.05–4.00 parts of a sweetener; 0.10–0.30 parts of an acidulant; purified water to make up to 100 parts; and the beverage is infused with carbon dioxide gas at a volume ratio of 3.5–4.5 V / V.

[0007] By adopting the above technical solution, the beverage system achieves synergistic control of physicochemical structural stability and physiological metabolic regulation. The specific reaction and mechanism of action are divided into the following steps: Step 1: Physical Isolation Process. The abundant hydrogen ions and high partial pressure carbon dioxide in the beverage base create a polar and acidic environment. The nanoliposomes construct a physical isolation barrier, confining the peptide molecules within the aqueous core of the vesicles, preventing contact between environmental hydrogen ions and the peptide's amide bonds, thus preventing peptide degradation and inactivation.

[0008] Step two: Gas-liquid interfacial tension regulation. Peptide macromolecules typically contain both hydrophobic and hydrophilic residues, which rapidly aggregate at the gas-liquid interface in their free state. Liposome encapsulation technology reduces the surface activity of the peptides in the dispersion medium. When the bottle is opened, causing a sudden drop in system pressure, dissolved carbon dioxide precipitates and forms bubbles. Because the surface tension of the system is not disturbed by the free peptides, the bubbles maintain their normal size and rupture in an orderly manner, preventing foam bursting. The foam stabilizer forms a viscoelastic monolayer film at the gas-liquid interface, prolonging the residence time of carbon dioxide in the liquid.

[0009] Step three: Physiological metabolic regulation process. After consumption, natural adaptogens participate in regulating the release of neurotransmitters in the central nervous system, alleviating oxidative stress in nerve cells; complex peptides and cell energy activators enter the bloodstream, activating the activity of alcohol dehydrogenase and lactate dehydrogenase, accelerating the degradation and excretion of acidic metabolites such as lactic acid, thus changing the way of stimulating the central nervous system with caffeine.

[0010] Preferably, the composite functional peptide composition encapsulated in nanoliposomes consists of a composite polypeptide core material and a liposome wall material, with a mass ratio of 1:5.0 to 1:5.5, and the average particle size of the composite functional peptide composition is less than 100 nm; the liposome wall material is composed of soybean lecithin and cholesterol in a mass ratio of 5:1; the composite polypeptide core material is composed of neuroprotective peptides, metabolic scavenging peptides, and antioxidant immune peptides in a mass ratio of 3-4:2-3:2-3.

[0011] By employing the above technical solution, lecithin and cholesterol at a mass ratio of 5:1 can form a dense phospholipid bilayer. Cholesterol molecules are embedded between the hydrophobic tail chains of phospholipids, restricting the freedom of movement of hydrocarbon chains and reducing membrane permeability. A core-to-wall ratio of 1:5.0 to 1:5.5 ensures sufficient wall material to completely encapsulate the internal aqueous phase and polypeptide molecules. Neuroprotective peptides, metabolic clearance peptides, and antioxidant immune peptides, combined in a predetermined ratio, exert their effects in the human body on three biochemical pathways: blood-brain barrier penetration, activation of liver metabolic enzymes, and inhibition of inflammatory factors, respectively.

[0012] Preferably, the neuroprotective peptide is walnut peptide or casein phosphopeptide; the metabolic clearance peptide is corn oligopeptide or soybean oligopeptide; and the antioxidant immune peptide is marine fish skin collagen tripeptide or oyster peptide.

[0013] By adopting the above technical solution, the small molecule peptides in the specific molecular weight range retain their bioactive structural domains, are not easily hydrolyzed into free amino acids in the gastrointestinal tract, and can be directly absorbed into the bloodstream through the small intestinal mucosa in the form of oligopeptides, thereby improving bioavailability.

[0014] Preferably, the natural adaptogen is a combination of one or more of L-theanine, γ-aminobutyric acid, and rhodioloside; the cell energy activator is a combination of one or more of water-soluble coenzyme Q10 powder, nicotinamide, encapsulated vitamin C microcapsule powder, and vitamin B12; and the foam stabilizer is a mixture of modified soap bark extract and glyceryl monostearate in equal mass.

[0015] By employing the above technical solution, triterpenoid saponins and glyceryl monostearate in the modified soap bark extract undergo molecular co-assembly under fluid shearing. The saponins provide steric hindrance, while the glyceryl monostearate increases the interfacial film thickness; together, they synergistically construct a stable microfoam network structure, controlling the gas exchange rate at the gas-liquid interface.

[0016] Preferably, the sweetener is one or a combination of two of erythritol or mogroside V; the acidulant is one or a combination of several of anhydrous citric acid, L-malic acid, and glucono-δ-lactone; the beverage also contains 0.10 to 2.00 parts by weight of flavoring substances, which are one or a combination of several of lemon verbena flavoring, ginger extract, L-menthol, and passion fruit concentrate.

[0017] By adopting the above technical solution, gluconate-δ-lactone undergoes slow hydrolysis in aqueous solution, gradually releasing gluconate, which forms a buffer system with anhydrous citric acid and L-malic acid, avoiding fluctuations in the pH value of the system and reducing the damage of the acidic environment to the external structure of liposomes.

[0018] Preferred method for preparing the composite functional peptide composition encapsulated in nanoliposomes is as follows: The polypeptides constituting the core material are mixed and dissolved in purified water to prepare an aqueous polypeptide solution; soybean lecithin and cholesterol are added to the polypeptide aqueous solution, and the mixture is dispersed uniformly by high-speed shearing and stirring to obtain a crude emulsion; the crude emulsion is fed into a high-pressure microfluidic homogenizer and continuously circulated 3-5 times under a pressure of 150-180 MPa to obtain a nanoliposome solution with an average particle size of less than 100 nm; the nanoliposome solution is then subjected to vacuum freeze-drying, pulverized, and sieved to obtain the powdered composite functional peptide composition.

[0019] By employing the above technical solution, the homogenizing equipment forces the crude emulsion through micron-sized pores under a pressure of 150 to 180 MPa. Utilizing mechanical shearing force, cavitation effect, and high-frequency impact, large-volume lipid droplets are broken up. Amphiphilic phospholipid molecules undergo self-assembly and rearrangement under mechanical energy input, encapsulating peptides in the aqueous phase. Vacuum freeze-drying, under low temperature and low pressure conditions, allows water to sublimate directly, avoiding peptide denaturation caused by high temperatures and the collapse and fusion of liposome vesicle structures, thus maintaining the reconstitution stability of the powdered composition.

[0020] Secondly, the present invention provides a method for preparing a beverage based on the synergistic targeted repair of complex functional peptides, using the following technical solution: A method for preparing a beverage based on the synergistic targeted repair of complex functional peptides includes the following steps: S1. Add the natural adaptogens, cell energy activators, foam stabilizers, sweeteners, and acidulants (excluding the compound functional peptide composition) to purified water according to the formula and stir to dissolve to obtain the beverage base liquid; S2. Cool the beverage base liquid; S3. A three-step method is used for aeration and mixing. First, carbon dioxide gas of 30% of the target carbon dioxide volume is introduced into the beverage base liquid to form a saturated base liquid. Then, a composite functional peptide composition solution that has been dissolved in a small amount of pure water is added while stirring. Finally, the remaining carbon dioxide gas is mixed online until the target volume is reached. S4. The entire process is carried out in a low-temperature, aseptic environment for cold filling to obtain the finished product.

[0021] By adopting the above technical solution, the manufacturing process ensures product stability from a physical processing perspective. Specifically: First, a peptide-free basic solution is constructed in the S1 stage to ensure that various electrolytes and flavor substances are homogeneously dispersed without interference from peptide surface activity. Secondly, in the S3 stage, 30% of the total carbon dioxide is dissolved first, establishing an initial carbonic acid buffer system and a certain level of dissolved gas partial pressure in the liquid phase. Finally, when liposomes are subsequently incorporated, the system has a basic pressure, which reduces the mechanical shear force exerted on the fluid by the online mixing equipment due to the introduced gas. This avoids the rupture of the liposome bilayer and leakage of peptide contents caused by high-intensity shear, thus maintaining the integrity of the nano-isolation structure.

[0022] Preferably, in step S2, the beverage base liquid is cooled to 1-4 degrees Celsius.

[0023] By adopting the above technical solution, the saturated solubility of carbon dioxide in water is increased by lowering the liquid phase temperature. Operating within the 1 to 4 degree Celsius range reduces the system operating pressure required to achieve the same carbonation volume multiple, further minimizing the physical stress damage to the liposome microstructure during the operation.

[0024] Preferably, in step S3, the stirring state when adding the composite functional peptide composition solution that has been dissolved in a small amount of purified water is a low-speed stirring state.

[0025] By adopting the above technical solution, the low-shear environment generated by low-speed stirring allows liposomes to be uniformly dispersed in the base liquid, while avoiding the strong shear force generated by local high-speed eddies that could damage the phospholipid molecules on the outside of the liposomes.

[0026] Preferably, in step S1, if the beverage components contain flavor substances, the flavor substances and other components such as natural adaptogens are added to purified water and stirred to dissolve.

[0027] By adopting the above technical solution, flavor substances are preferentially added to the pure aqueous phase system to complete the hydration of polar components and the dispersion of volatile components in the solvent network, thus avoiding the subsequent high-pressure state from hindering the uniform diffusion of flavor molecules.

[0028] This invention provides a beverage based on the synergistic targeted repair of complex functional peptides and its preparation method. It possesses the following beneficial effects: 1. This invention employs nanoliposome encapsulation technology to process complex functional peptides, isolating polypeptide molecules within the aqueous phase of vesicles and severing direct contact between the polypeptides and the gas-liquid interface in the external carbonated liquid. This physical isolation barrier eliminates the surface activity of the polypeptide macromolecules, solving the problem of foam overflow easily caused by adding polypeptides to carbonated beverages. Simultaneously, it blocks the degradation of polypeptide molecules by the acidic environment, masks the inherent fishy and astringent odor of the polypeptides, and improves the physical stability and sensory acceptability of the beverage.

[0029] 2. This invention constructs a compound system composed of neuroprotective peptides, metabolic clearance peptides, and antioxidant immune peptides, combined with natural adaptogens and cellular energy activators. After entering the human body, the formula components work synergistically against biochemical pathways such as the blood-brain barrier, liver metabolic enzymes, and inflammatory factors, accelerating the degradation and excretion of acidic metabolites such as lactic acid. This combination of components changes the traditional functional beverage model that relies on stimulants to mask fatigue signals, maintaining concentration without interfering with the body's normal circadian rhythm, and promoting the functional recovery of the nervous and metabolic systems.

[0030] 3. This invention employs a three-step gradient aeration process that pre-establishes a basic carbonic acid partial pressure, combined with a foam stabilizer composed of modified soap bark extract and glyceryl monostearate. Gradient aeration and low-temperature operation reduce the mechanical shear force exerted on the fluid by the gas mixing equipment, preventing high-intensity shear from damaging the liposome bilayer structure and ensuring the integrity of the nano-encapsulated microparticles. The foam stabilizer components co-assemble at the gas-liquid interface to form a network structure, causing the carbon dioxide released after opening to form a fine, micro-foam layer, prolonging the gas retention time and improving the product's drinking taste. Attached Figure Description

[0031] Figure 1 This is a flowchart of the preparation method of the present invention; Figure 2 This is a flowchart of the nanoliposome encapsulation process of the composite functional peptide composition of the present invention. Detailed Implementation

[0032] The main raw materials and reagents used in the following examples and comparative examples have the following sources and specifications. Reagents not specifically mentioned are all commercially available analytical grade or higher grade products.

[0033] Walnut peptides, commercially available food grade, with an oligopeptide mass fraction of more than 80% having a relative molecular mass of less than 1000 Da.

[0034] Casein phosphopeptide, commercially available food grade, CAS number 691364-49-5, purity greater than 90%.

[0035] Corn oligopeptides, commercially available food grade, with a mass fraction of more than 85% short-chain peptides with a relative molecular mass of less than 1000 Da.

[0036] Soybean oligopeptides, commercially available food grade, with a mass fraction of more than 85% of the components having a relative molecular mass of less than 1000 Da.

[0037] Marine fish skin collagen tripeptide, commercially available food grade, is a low molecular weight polypeptide with a glycine-proline-hydroxyproline sequence as the main component, and an average relative molecular mass distribution between 280 Da and 500 Da.

[0038] Oyster peptides, commercially available food grade, are extracted from fresh oyster meat using bio-enzymatic hydrolysis technology, with more than 80% oligopeptides having a relative molecular mass of less than 1000 Da.

[0039] Soy lecithin, commercially available food grade, CAS number 8002-43-5, with a phosphatidylcholine content greater than 70%.

[0040] Cholesterol, commercially available food grade, CAS number 57-88-5, purity greater than 95%.

[0041] Modified soap bark extract, commercially available food grade, CAS number 68990-67-0, with a saponin content greater than 20%.

[0042] Glyceryl monostearate, commercially available food grade, CAS number 123-94-4, purity greater than 90%.

[0043] Water-soluble coenzyme Q10, commercially available food grade, is a water-soluble powder prepared using γ-cyclodextrin inclusion technology. The CAS number of the coenzyme Q10 inside is 303-98-0, and the effective ingredient mass fraction is 10%.

[0044] Encapsulated vitamin C, commercially available food-grade microcapsule powder, core material ascorbic acid CAS number 50-81-7, mass fraction of 90%, wall material is gelatin.

[0045] Reference Figure 2 , Figure 2 This is a flowchart of the nanoliposome encapsulation process for the composite functional peptide composition of the present invention; Preparation Example 1: This preparation example provides a composite functional peptide composition encapsulated in nanoliposomes, comprising the following steps: Weigh out 4g of walnut peptide, 3g of corn oligopeptide, and 2g of marine fish skin collagen tripeptide. Mix the three and dissolve them in 1000g of purified water to prepare a polypeptide aqueous solution. Add 40g of soybean lecithin and 8g of cholesterol to the polypeptide aqueous solution and disperse evenly by high-speed shearing and stirring to obtain a crude emulsion. Input the crude emulsion into a high-pressure microfluidic homogenizer and continuously cycle it three times under a pressure of 150MPa to obtain a nanoliposome solution with an average particle size of less than 100nm. Vacuum freeze-dry the nanoliposome solution, pulverize and sieve it to obtain a powdered composite functional peptide composition.

[0046] Preparation Example 2: This preparation example provides a composite functional peptide composition encapsulated in nanoliposomes, comprising the following steps: Weigh out 4g of soybean oligopeptide, 2g of oyster peptide, and 3g of walnut peptide, mix them, and dissolve them in 1000g of purified water to prepare a polypeptide aqueous solution. Add 40g of soybean lecithin and 8g of cholesterol to the polypeptide aqueous solution, and disperse evenly by high-speed shearing and stirring to obtain a crude emulsion. Input the crude emulsion into a high-pressure microfluidic homogenizer and continuously cycle it 5 times under a pressure of 180MPa to obtain a nanoliposome solution with an average particle size of less than 100nm. Vacuum freeze-dry the nanoliposome solution, pulverize and sieve it to obtain a powdered composite functional peptide composition.

[0047] Preparation Example 3: This preparation example provides a composite functional peptide composition encapsulated in nanoliposomes, comprising the following steps: Weigh out 3g of marine fish skin collagen tripeptide, 3g of corn oligopeptide, and 3g of walnut peptide, mix them, and dissolve them in 1000g of purified water to prepare a polypeptide aqueous solution. Add 40g of soybean lecithin and 8g of cholesterol to the polypeptide aqueous solution, and disperse evenly by high-speed shearing and stirring to obtain a crude emulsion. Input the crude emulsion into a high-pressure microfluidic homogenizer and continuously cycle it 4 times under a pressure of 165MPa to obtain a nanoliposome solution with an average particle size of less than 100nm. Vacuum freeze-dry the nanoliposome solution, pulverize and sieve it to obtain a powdered composite functional peptide composition.

[0048] Reference Figure 1 , Figure 1 A process flow diagram for preparing a beverage based on synergistic targeted repair of complex functional peptides provided by the present invention; Example 1: This embodiment provides an anti-late-night functional carbonated beverage based on the synergistic targeted repair of compound functional peptides and its preparation method, including the following steps: weighing the formula raw materials by weight, including 0.40 kg of the compound functional peptide composition obtained in Preparation Example 1, 0.06 kg of L-theanine, 0.02 kg of γ-aminobutyric acid, 0.001 kg of total vitamin B12, 3.50 kg of erythritol, 0.08 kg of foam stabilizer (a mixture of modified soap bark extract and glyceryl monostearate in equal mass), 0.10 kg of lemon verbena flavoring, and 0.20 kg of acidulant (a mixture of anhydrous citric acid and L-malic acid in equal mass). The remainder is purified water, with a total mass of 100 kg. All raw materials except the composite functional peptide composition are added to purified water and stirred to dissolve, obtaining the beverage base liquid. The beverage base liquid is cooled to 2 degrees Celsius. A three-step aeration and mixing method is used: first, carbon dioxide gas at 30% of the target carbon dioxide volume ratio is introduced into the base liquid to form a saturated base liquid; then, the above-mentioned composite functional peptide composition solution, which has been dissolved in a small amount of purified water beforehand, is added under low-speed stirring; finally, the remaining carbon dioxide gas is mixed online until the target volume ratio is 4.0 V / V; the entire process is carried out in a low-temperature aseptic environment for cold filling to obtain the finished product.

[0049] Example 2: This embodiment provides an anti-late-night functional carbonated beverage based on the synergistic targeted repair of compound functional peptides and its preparation method, including the following steps: weighing the formula raw materials by weight, including 0.50 kg of the compound functional peptide composition obtained in Preparation Example 2, 0.05 kg of water-soluble coenzyme Q10 powder, 0.03 kg of rhodioloside, 0.20 kg of ginger extract, 0.015 kg of L-menthol, 0.05 kg of total mogroside V, 0.10 kg of foam stabilizer (a mixture of modified soap bark extract and glyceryl monostearate in equal mass), and 0.1 kg of acidulant (a mixture of anhydrous citric acid and glucono-δ-lactone in equal mass). 5 kg of raw materials were prepared, with the remainder being purified water, for a total mass of 100 kg. All raw materials except the composite functional peptide composition were added to the purified water and stirred to dissolve, thus obtaining the beverage base liquid. The beverage base liquid was cooled to 3 degrees Celsius. A three-step aeration and mixing method was used: first, carbon dioxide gas at 30% of the target carbon dioxide volume ratio was introduced into the base liquid to form a saturated base liquid; then, the above-mentioned composite functional peptide composition solution, which had been dissolved in a small amount of purified water beforehand, was added under low-speed stirring; finally, the remaining carbon dioxide gas was mixed online until the target volume ratio was 4.2V / V. The entire process was carried out in a low-temperature aseptic environment for cold filling to obtain the finished product.

[0050] Example 3: This embodiment provides an anti-late-night functional carbonated beverage based on the synergistic targeted repair of compound functional peptides and its preparation method, including the following steps: weighing the formula raw materials by weight, including 0.45 kg of the compound functional peptide composition obtained in Preparation Example 3, 0.02 kg of γ-aminobutyric acid, 0.03 kg of nicotinamide, 0.10 kg of encapsulated vitamin C microcapsule powder, 2.00 kg of passion fruit concentrate, 3.00 kg of erythritol, 0.09 kg of foam stabilizer (a mixture of modified soap bark extract and glyceryl monostearate in equal mass), and 0.15 kg of acidulant (a mixture of L-malic acid and glucono-δ-lactone in equal mass). The remainder is purified water, with a total mass of 100 kg. All raw materials except the composite functional peptide composition are added to purified water and stirred to dissolve, obtaining the beverage base liquid. The beverage base liquid is cooled to 1 degree Celsius. A three-step aeration and mixing method is used: first, carbon dioxide gas at 30% of the target carbon dioxide volume ratio is introduced into the base liquid to form a saturated base liquid; then, the above-mentioned composite functional peptide composition solution, which has been dissolved in a small amount of purified water beforehand, is added under low-speed stirring; finally, the remaining carbon dioxide gas is mixed online until the target volume ratio is 3.8V / V; the entire process is carried out in a low-temperature aseptic environment for cold filling to obtain the finished product.

[0051] Example 4: This embodiment provides an anti-late-night functional carbonated beverage based on the synergistic targeted repair of compound functional peptides and its preparation method, including the following steps: Weighing the formula raw materials by weight, including 0.20 kg of the compound functional peptide composition obtained in Preparation Example 1, 0.01 kg of L-theanine, 0.02 kg of water-soluble coenzyme Q10 powder, 2.00 kg of erythritol, 0.05 kg of foam stabilizer mixed with modified soap bark extract and glyceryl monostearate in equal mass, 0.10 kg of anhydrous citric acid, and the remainder being purified water, with a total mass of 100 kg; All raw materials except the composite functional peptide composition are added to purified water and stirred to dissolve, thus obtaining a beverage base liquid. The beverage base liquid is cooled to 1 degree Celsius. A three-step aeration and mixing process is adopted: first, carbon dioxide gas at 30% of the target carbon dioxide volume ratio is introduced into the base liquid to form a saturated base liquid; then, the above-mentioned composite functional peptide composition solution, which has been dissolved in a small amount of purified water beforehand, is added under low-speed stirring; finally, the remaining carbon dioxide gas is mixed online until the target volume ratio is 3.5V / V; the entire process is carried out in a low-temperature aseptic environment for cold filling to obtain the finished product.

[0052] Example 5: This embodiment provides an anti-late-night functional carbonated beverage based on the synergistic targeted repair of compound functional peptides and its preparation method, including the following steps: Weighing the formula raw materials by weight, including 0.60 kg of the compound functional peptide composition obtained in Preparation Example 2, 0.08 kg of γ-aminobutyric acid, 0.10 kg of nicotinamide, 4.00 kg of erythritol, 0.15 kg of foam stabilizer (a mixture of modified soap bark extract and glyceryl monostearate in equal mass), 0.30 kg of L-malic acid, and the remainder being purified water, with a total mass of 100 kg; [The text abruptly ends here, likely due to an incomplete sentence or missing information.] All raw materials except the peptide composition are added to purified water and stirred to dissolve, thus obtaining a beverage base liquid. The beverage base liquid is cooled to 4 degrees Celsius. A three-step aeration and mixing process is adopted. First, carbon dioxide gas of 30% of the target carbon dioxide volume is introduced into the base liquid to form a saturated base liquid. Then, the above-mentioned composite functional peptide composition solution, which has been dissolved in a small amount of purified water beforehand, is added under low-speed stirring. Finally, the remaining carbon dioxide gas is mixed online until the target volume ratio is 4.5V / V. The entire process is carried out in a low-temperature aseptic environment for cold filling to obtain the finished product.

[0053] Comparative Example 1: Compared with Example 1, the difference is that the composite functional peptide composition encapsulated by nanoliposomes obtained in Preparation Example 1 was not used. Instead, a mixture of free walnut peptide, corn oligopeptide and marine fish skin collagen tripeptide with the same content of pure peptides as in the composite functional peptide composition was directly added (the mass ratio of the three peptides was the same as in Preparation Example 1, i.e., 4:3:2).

[0054] Comparative Example 2: Compared with Example 1, the difference is that the foam stabilizer made of modified soap bark extract and glyceryl monostearate was removed from the formula, and the difference was made up with an equal mass of purified water; otherwise, they are the same.

[0055] Comparative Example 3: Compared with Example 1, the difference lies in the change of the synergistic system of the core complex functional peptides. The liposome powder used in the preparation process removed the walnut peptide that has a neuroprotective effect. Instead, it was prepared by encapsulating corn oligopeptide and marine fish skin collagen tripeptide in a mass ratio of 3:2 with equal total mass. All other aspects were the same.

[0056] Comparative Example 4: Compared to Example 1, the difference is that the natural adaptogens L-theanine and γ-aminobutyric acid were removed from the formula, and the removed L-theanine and γ-aminobutyric acid were replaced by an equal mass of caffeine and taurine commonly used in commercially available functional beverages. All other aspects are the same.

[0057] Comparative Example 5: Compared with Example 1, the difference is that the three-step gas filling and mixing method was not used in the preparation process. Instead, after the composite functional peptide composition solution was added to the base liquid, all carbon dioxide gas was filled into the mixture under high pressure online to the target volume multiple of 4.0V / V. All other aspects are the same.

[0058] Test Example 1: Take 2 mL of each of the undried nanoliposome solutions obtained in Preparation Examples 1 to 3, dilute with deionized water, and transfer to the sample cell of a dynamic light scattering instrument. Set the test temperature to 25°C, equilibrate for 120 seconds, and then start the scan. Perform three parallel measurements for each batch of samples, and record the average particle size and polydispersity index output by the instrument. Take 5 mL of each of the nanoliposome solutions obtained in Preparation Examples 1 to 3 and transfer to the inner tube of an ultrafiltration centrifuge tube with a molecular weight cutoff of 10 kDa. Set the centrifuge parameters to 4°C and 10,000 rpm, and run for 30 minutes. Collect the filtrate in the outer tube of the centrifuge tube. Determine the mass concentration of free peptides in the filtrate using high-performance liquid chromatography (HPLC). Separately, take an equal volume of the original nanoliposome solution, add 10% (v / v) Triton X-100 surfactant solution for demulsification, and then use ultrasonic vibration to break down the liposome bilayer structure, releasing the encapsulated peptides. Determine the total peptide mass concentration. The embedding rate is calculated by dividing the difference between the total peptide mass concentration and the free peptide mass concentration by the total peptide mass concentration and then multiplying by 100%.

[0059] Table 1. Test data on the physicochemical properties of composite functional peptide nanoliposomes

[0060] High-pressure microfluidic homogenizers apply shear force, cavitation effect, and high-frequency impaction to fluids through micron-sized channels. Soybean lecithin and cholesterol undergo structural rearrangement under mechanical stress in an aqueous system, constructing a bilayer vesicle system. Shear strength determines the formation quality of liposomes and the loading efficiency of internal water-soluble substances.

[0061] Table 1 shows that the average particle size of the vesicles obtained in Preparation Examples 1 to 3 is less than 100 nm. Preparation Example 2 was treated continuously for 5 times under a pressure of 180 MPa, resulting in the maximum mechanical energy input to the fluid, sufficient droplet fragmentation and recombination within the system, an average particle size of 75.8 nm, a polydispersity index of 0.163, and a uniform vesicle particle size distribution. The encapsulation rate of Preparation Example 2 is 89.2%, indicating that under the condition of a total core material peptide to wall material mass ratio of approximately 1:5.3, the wall material molecules are sufficient to construct a phospholipid bilayer structure, encapsulating water-soluble peptide molecules in the aqueous core region inside the vesicles. Preparation Example 1 had a homogenization pressure and number of treatments at the lower limit, resulting in a relatively small total shear work received by the system. Some vesicles showed fusion or incomplete refinement, with a particle size of 94.3 nm. The free peptide content increased, and the encapsulation rate was 81.6%. All physicochemical properties met the basic requirements of a nano-encapsulation system.

[0062] Characterization data demonstrate that the preparation process achieves physical isolation of the complex peptides. This physical encapsulation barrier reduces the probability of hydrophobic groups in the peptide molecules being exposed to external polar solvents, inhibits the tendency of peptides to accumulate at the gas-liquid interface, and alters the surface tension parameters of the system. This provides a structural basis for controlling foaming when formulating beverages with high carbon dioxide partial pressure.

[0063] Test Example 2: This test case aims to examine the physiological intervention effects of the embodiment and comparative products in a night shift scenario.

[0064] Experimental Procedure: Sixty consecutive night shift workers were recruited and randomly divided into four groups for a four-week intervention test. Group 1 drank the beverage obtained in Example 1; Group 2 drank the beverage obtained in Comparative Example 3; Group 3 drank the beverage obtained in Comparative Example 4; and Group 4 drank ordinary soda water as a blank control. The frequency of consumption was one can per night.

[0065] Cognitive ability test: The reaction time and memory accuracy of the subjects at the end of the night shift were recorded using a sustained attention test system.

[0066] Biochemical index testing: Venous blood was collected from subjects in the morning after their night shift to measure serum lactate concentration and cortisol level.

[0067] Sleep quality test: Subjects wore smart sleep monitoring devices to record the latency period and the percentage of deep sleep time when they first fell asleep after get off work.

[0068] Table 2 Comprehensive Test Data of Physiological Intervention

[0069] Test data showed that there were no significant differences in reaction time and accuracy between the first and third groups at the end of the night shift, both being superior to the fourth group. This indicates that the composition in Example 1 achieved a cognitive arousal effect comparable to commercially available caffeine mixtures. The second group, lacking walnut peptides, had worse reaction time and accuracy than the first group, demonstrating the necessity of neuroprotective peptides in the compound system for maintaining cognitive function.

[0070] In terms of biochemical metabolism and sleep indicators, the first group had the lowest serum lactate and cortisol concentrations, the shortest sleep latency, and the highest proportion of deep sleep. The third group, affected by caffeine, experienced persistently high cortisol levels after the night shift, low lactate clearance, a significantly prolonged sleep latency to 42.3 minutes, and a decreased proportion of deep sleep. Example 1 utilizes metabolic clearance peptides to activate metabolic enzymes and accelerate lactate excretion, combined with the calming effect of natural adaptogens on the central nervous system, avoiding the side effects of traditional energy drinks on subsequent sleep.

[0071] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A beverage based on the synergistic targeted repair of complex functional peptides, characterized in that, The beverage contains the following components by weight: 0.20–0.60 parts of a composite functional peptide composition encapsulated in nanoliposomes; Natural adaptogens: 0.01–0.08 parts; Cellular energy activator 0.001–0.13 parts; Foam stabilizer 0.05-0.15 parts; Sweetener 0.05–4.00 parts; Acidulant: 0.10–0.30 parts; Add purified water to bring the total to 100 servings; Furthermore, the beverage is filled with carbon dioxide gas at a volume ratio of 3.5 to 4.5 V / V.

2. The beverage based on synergistic targeted repair of complex functional peptides according to claim 1, characterized in that, The composite functional peptide composition encapsulated in nanoliposomes consists of a composite polypeptide core material and a liposome wall material, wherein the mass ratio of the composite polypeptide core material to the liposome wall material is 1:5.0 to 1:5.5, and the average particle size of the composite functional peptide composition is less than 100 nm. The liposome wall material is composed of soybean lecithin and cholesterol in a mass ratio of 5:1; The composite polypeptide core material is composed of neuroprotective peptides, metabolic scavenging peptides, and antioxidant immune peptides in a mass ratio of 3-4:2-3:2-3.

3. The beverage based on synergistic targeted repair of complex functional peptides according to claim 2, characterized in that, The neuroprotective peptide is walnut peptide or casein phosphopeptide. The metabolic clearance peptide is a corn oligopeptide or a soybean oligopeptide. The antioxidant immune peptide is marine fish skin collagen tripeptide or oyster peptide.

4. The beverage based on synergistic targeted repair of complex functional peptides according to claim 1, characterized in that, The natural adaptogen is one or a combination of several of L-theanine, γ-aminobutyric acid, and rhodioloside; The cell energy activator is one or a combination of several of the following: water-soluble coenzyme Q10 powder, nicotinamide, encapsulated vitamin C microcapsule powder, and vitamin B12. The foam stabilizer is composed of a mixture of modified soap bark extract and glyceryl monostearate in equal mass.

5. The beverage based on synergistic targeted repair of complex functional peptides according to claim 1, characterized in that, The sweetener is one or a combination of two of erythritol or mogroside V; The acidulant is one or a combination of several of anhydrous citric acid, L-malic acid, and glucono-δ-lactone. The beverage also contains 0.10 to 2.00 parts by weight of flavoring substances, which are one or more of lemon verbena flavoring, ginger extract, L-menthol, and passion fruit concentrate.

6. The beverage based on synergistic targeted repair of complex functional peptides according to claim 1, characterized in that, The specific preparation method of the composite functional peptide composition encapsulated in nanoliposomes is as follows: The polypeptides constituting the core material are mixed and dissolved in pure water to prepare a polypeptide aqueous solution; soybean lecithin and cholesterol are added to the polypeptide aqueous solution, and the mixture is dispersed evenly by high-speed shearing and stirring to obtain a crude emulsion; the crude emulsion is fed into a high-pressure microfluidic homogenizer and continuously circulated 3 to 5 times under a pressure of 150 to 180 MPa to obtain a nanoliposome solution with an average particle size of less than 100 nm; the nanoliposome solution is subjected to vacuum freeze-drying, pulverized and sieved to obtain a powdered composite functional peptide composition.

7. The preparation method of the beverage based on the synergistic targeted repair of complex functional peptides according to any one of claims 1-6, characterized in that, Includes the following steps: S1. Add the natural adaptogens, cell energy activators, foam stabilizers, sweeteners, and acidulants (excluding the compound functional peptide composition) to purified water according to the formula and stir to dissolve to obtain the beverage base liquid; S2. Cool the beverage base liquid; S3. A three-step method is used for aeration and mixing. First, carbon dioxide gas at 30% of the target carbon dioxide volume is introduced into the beverage base liquid to form a saturated base liquid. Then, the composite functional peptide composition solution, which has been dissolved in a small amount of pure water beforehand, is added while stirring. Finally, the remaining carbon dioxide gas is mixed online until the target volume is reached. S4. The entire process is carried out in a low-temperature, aseptic environment for cold filling to obtain the finished product.

8. The preparation method of the beverage based on the synergistic targeted repair of complex functional peptides as described in claim 7, characterized in that, In step S2, the beverage base liquid is cooled to 1-4 degrees Celsius.

9. The preparation method of the beverage based on the synergistic targeted repair of complex functional peptides as described in claim 7, characterized in that, In step S3, the stirring state when adding the composite functional peptide composition solution that has been dissolved in a small amount of purified water is a low-speed stirring state.

10. The preparation method of the beverage based on the synergistic targeted repair of complex functional peptides according to claim 7, characterized in that, In step S1, if the beverage components contain flavor substances, the flavor substances and other components such as natural adaptogens are added to purified water and stirred to dissolve.

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