A composition for repairing lung tissue damage after viral infection, and a preparation method and application thereof

Abstract

The present application belongs to the technical field of functional food and dietary nutritional supplement, and particularly relates to a composition for repairing lung tissue damage after viral infection and a preparation method and application thereof, wherein the composition comprises yam powder, giant salamander small molecule peptide powder, pueraria powder, lily powder, needle leaf cherry powder, yeast beta-glucan, red date powder, platycodon grandiflorum extract, salvia miltiorrhiza extract, curcuma longa extract, freeze-dried naoto powder, houttuynia cordata extract and fucoidan. The present application utilizes giant salamander small molecule peptides with D50 of 12.5-18.2 microns, yam powder rich in amylose and pueraria powder to form compact particle packing grading of large particles + small particles in a specific ratio, improves the compatibility of multiple components, and reduces the hygroscopic and caking tendency of the powder in the storage process. In combination with the step-by-step mixing process, the flowability and physical stability of the powder are significantly improved. The composition has good physical stability and high uniformity, and can be used for preparing dietary nutritional supplement products for the recovery period of viral infection.

Description

Technical Field

[0001] This invention belongs to the technical field of functional foods and dietary supplements, specifically referring to a composition for repairing lung tissue damage after viral infection, its preparation method, and its application. Background Technology

[0002] After a viral infection, especially a respiratory viral infection, the body has comprehensive dietary nutritional needs for protein, polysaccharides, vitamins, and plant-derived nutrients during the recovery phase. These needs can be met through reasonable dietary supplementation. Currently, most recovery nutrition products on the market are single-component and cannot meet the needs of multiple sources of nutrition; while compound products are usually just simple physical dry mixing.

[0003] However, in the development of multi-source composite nutrient powders, significant physical differences exist in particle size distribution, bulk density, hygroscopicity, and surface adhesion among raw materials from different sources (plant polysaccharide powder, animal protein peptide powder, and highly active plant extracts). This leads to physical stability problems in the powder system, such as poor mixing uniformity, easy moisture absorption and clumping, and local aggregation of trace odor components. 1. Moisture absorption and clumping of polysaccharide and protein powders: Plant powders such as yam powder and kudzu root powder, which are rich in amylose and mucilage, are highly hygroscopic. When mixed with conventional protein powder, due to the lack of reasonable packing gradation between particles, they are very easy to absorb trace amounts of moisture from the environment during storage, causing liquid bridging between particles and clumping, which seriously reduces the flowability of the powder and its shelf life quality. 2. Agglomeration and flavor deterioration of trace characteristic extracts: Plant-derived ingredients such as turmeric extract and platycodon extract are added in extremely small amounts (usually less than 3% of the total weight) and have strong bitter and spicy characteristic odors. During the dry mixing process, due to the strong electrostatic adsorption and agglomeration tendency of trace extracts, traditional direct dry mixing can easily lead to local enrichment of trace components, causing batch-to-batch uniformity to exceed the standard and producing strong local unpleasant odors in the product; 3. Powder stratification caused by differences in density and particle size: Small molecule peptide powder and natural plant powder have significant differences in density and flowability. Conventional dry mixing processes are difficult to form a uniform and stable compound system, and gravity stratification is prone to occur during transportation and storage.

[0004] Therefore, the core of this invention lies in providing a compound nutritional composition suitable for nutritional supplementation during the recovery phase of viral infection. By utilizing a high proportion of plant polysaccharide powder and low molecular weight peptide powder with specific molecular weight / particle size to form a stable compound particle packing gradation system, and combining proportion control with a pre-dilution mixing process of trace extract carrier, the physical stability, mixing uniformity and flavor characteristics of multi-source nutritional powder are improved.

[0005] In this application, "repair" specifically refers to meeting the body's nutritional needs through dietary supplementation during the recovery phase of viral infection. Its technical effect is reflected in nutritional support, rather than pharmacological treatment or medical intervention targeting pathological damage to lung tissue.

[0006] The "lung tissue damage repair" in this application mainly targets the body's recovery needs due to nutritional consumption during the recovery phase of viral infection. Its technical effect is to provide the nutritional support required during the recovery phase, rather than to treat or medically repair pathological damage to lung tissue. Summary of the Invention

[0007] To address the aforementioned challenges, this invention provides a composition for repairing lung tissue damage following viral infection, its preparation method, and its application.

[0008] To achieve the above functions, the technical solution adopted by the present invention is as follows: A composition for repairing lung tissue damage after viral infection, comprising the following components in parts by weight: 40-120 parts of yam powder, 40-120 parts of giant salamander small molecule peptide powder, 30-90 parts of kudzu root powder, 25-80 parts of lily powder, 20-60 parts of acerola cherry powder, 10-40 parts of yeast β-glucan, 10-40 parts of jujube powder, 5-20 parts of platycodon root extract, 5-20 parts of salvia miltiorrhiza extract, 5-20 parts of turmeric extract, 5-20 parts of natto freeze-dried powder, 4-15 parts of houttuynia cordata extract, and 2-10 parts of fucoidan; wherein the average molecular weight of the giant salamander small molecule peptide powder is less than 1000 Daltons.

[0009] In a preferred embodiment of the present invention, the weight ratio of yam powder to giant salamander small molecule peptide powder is 1:0.33 to 1:3, preferably 1:0.5 to 1:1.5, and most preferably 1:1. The average molecular weight of the giant salamander small molecule peptide powder is controlled below 1000 Daltons, and its measured median particle size (D50) is 12.5 to 18.2 μm. The median particle size (D50) of the plant matrix powders such as yam powder and kudzu root powder is 75 to 110 μm. By controlling the particle size difference between the giant salamander small molecule peptide powder and the plant matrix powder, the small-particle peptide powder can be distributed in the gaps between the plant powder particles, improving the particle size distribution of the composite powder, reducing the tendency of agglomeration, stratification, and hygroscopic clumping during the mixing of powders from different sources, and improving the uniformity and stability of the composite powder system. Through experiments, the inventors discovered that when the median particle size (D50) of the giant salamander small molecule peptide powder is less than 10 μm, the powder is prone to electrostatic adsorption and agglomeration during mixing. When the D50 is greater than 25 μm, its particle size is close to or exceeds the interstitial size of the plant matrix powder, making it difficult to effectively embed into the skeletal voids formed by large particles. Controlling the D50 of the giant salamander small molecule peptide powder to 12.5–18.2 μm can achieve a better interstitial filling effect.

[0010] As a preferred embodiment of the present invention, the composition further includes auxiliary nutritional components: 2-10 parts of zinc-enriched yeast powder, 2-10 parts of defatted flaxseed powder, and 2-10 parts of ultrafine rice bran powder.

[0011] As a preferred embodiment of the present invention, the composition further includes antioxidant and mitochondrial nutritional components: 0.5-3 parts of ergothionein and 0.2-2 parts of pyrrolizidine quinoline quinone disodium salt (PQQ).

[0012] As a preferred embodiment of the present invention, the composition further includes 0.5 to 3 parts of mogroside and 0.1 to 1 part of natural vitamin D3 powder.

[0013] As a preferred embodiment of the present invention, the composition comprises, by weight, 80 parts of yam powder, 80 parts of giant salamander small molecule peptide powder, 60 parts of kudzu root powder, 50 parts of lily powder, 40 parts of acerola cherry powder, 20 parts of yeast β-glucan, 20 parts of jujube powder, 10 parts of platycodon root extract, 10 parts of salvia miltiorrhiza extract, 10 parts of turmeric extract, 10 parts of natto freeze-dried powder, 8 parts of houttuynia cordata extract, 6 parts of zinc-enriched yeast powder, 5 parts of defatted flaxseed powder, 5 parts of ultrafine rice bran powder, 5 parts of fucoidan, 1 part of ergothioneine, 1 part of disodium pyrroquinone, 1 part of mogroside, and 0.5 parts of natural vitamin D3 powder.

[0014] The present invention also discloses a method for preparing a composition for repairing lung tissue damage after viral infection, comprising the following steps: S1 Raw Material Pretreatment: Yam powder, kudzu root powder, lily powder, acerola cherry powder, jujube powder, natto freeze-dried powder, defatted flaxseed powder, and ultrafine rice bran powder are dried at a temperature of 40-70℃ to ensure that the moisture content of the raw materials is ≤8%; Giant salamander small molecule peptide powder and plant extracts are processed through a 60-100 mesh sieve. S2 Plant Extract Pre-dilution Mixing: Platycodon grandiflorus extract, Salvia miltiorrhiza extract, Curcuma longa extract and Houttuynia cordata extract are mixed according to the formula, and porous carrier powder accounting for 50% to 100% of the total weight of the extracts is added, that is, the mass ratio of plant extracts to porous carrier powder is 1:0.5 to 1:1. The porous carrier powder is ultrafine rice bran powder or defatted flaxseed powder with an average particle size of 80 to 200 mesh. The mixture is premixed at a speed of 10 to 20 r / min for 10 to 20 minutes to obtain the plant extract pre-dilution mixture. S3 Basic Powder Mixing: Add the pretreated plant powder raw materials, giant salamander small molecule peptide powder and the pre-diluted mixture of plant extracts obtained in step S2 to the mixing equipment and mix at a speed of 8-20 r / min for 15-40 minutes to obtain the basic mixed powder. S4 Functional Component Compound: Add yeast β-glucan, fucoidan, auxiliary nutrients, antioxidant and mitochondrial nutrients, mogrosides and natural vitamin D3 powder to the basic mixed powder, and continue mixing for 15-40 minutes to obtain the compound powder. S5 Formulation: The composite powder is prepared into powder, granules, capsules or tablets.

[0015] The present invention also provides the application of the above-mentioned composition for repairing lung tissue damage after viral infection in the preparation of dietary nutritional supplement products for the recovery stage, wherein the recovery stage is the dietary nutritional recovery stage after viral infection; the dietary nutritional supplement products are functional foods, dietary nutritional supplements or special dietary foods; the dosage form of the composition includes powders, granules, capsules or tablets.

[0016] Compared with the prior art, the present invention achieves the following beneficial effects by adopting the above structure: By combining small molecule peptides of giant salamander with a D50 of 12.5–18.2 μm with yam powder and kudzu root powder rich in amylose in a specific ratio, a compact particle packing gradation of "large particles + small particles" was formed, which improved the compatibility of multiple components and reduced the tendency of powder to absorb moisture and clump during storage; combined with a stepwise mixing process, the flowability and physical stability of the powder were significantly improved. By adding 80-200 mesh ultrafine rice bran powder or defatted flaxseed powder as a porous carrier in step S2, the extracts of Platycodon grandiflorus, Salvia miltiorrhiza, Curcuma longa and Houttuynia cordata with low addition amount and strong characteristic odor are pre-diluted and dispersed. The microscopic physical adsorption of the porous carrier effectively adsorbs and isolates characteristic odor components, which solves the problem of local aggregation of multi-source trace plant extracts in the composite powder. This significantly reduces the coefficient of variation (CV%) of indicator components such as curcumin, and achieves highly uniform dispersion and good palatability. This invention systematically combines plant powders, bioactive peptides, polysaccharide nutrients, mitochondrial metabolic cofactors (PQQ and ergothioneine), and natural vitamin sources to form a dietary nutrient composition that is diverse in origin, rationally composed, and physically stable, providing nutritional support for the recovery phase of viral infection. Detailed Implementation

[0017] The present invention will be further described in detail below with reference to specific embodiments, but the embodiments of the present invention are not limited thereto.

[0018] Raw material description: Giant salamander small molecule peptide powder: It is prepared by using protein from giant salamander as raw material through enzymatic hydrolysis, filtration, concentration and drying processes. The average molecular weight is less than 1000 Daltons and the measured D50 is 15.2μm.

[0019] Yam powder / Kudzu root powder: conventional food-grade dry powder, with measured D50 values ​​of 82.5μm and 88.1μm, respectively.

[0020] Platycodon grandiflorus extract: obtained by water extraction, concentration and drying of Platycodon grandiflorus root, with a concentration ratio of 10:1.

[0021] Salvia miltiorrhiza extract: obtained by extraction, concentration and drying of Salvia miltiorrhiza root, with a concentration ratio of 10:1.

[0022] Turmeric extract: obtained by extraction and drying of turmeric rhizomes, with a curcumin content ≥10%.

[0023] Houttuynia cordata extract: obtained by water extraction, concentration and drying of the above-ground parts of Houttuynia cordata, with a concentration ratio of 10:1.

[0024] PQQ (pyrroloquinoline quinone disodium salt): High-purity food-grade raw material, purity ≥98%.

[0025] Example 1: Basic Composite Composition This embodiment provides a composition for repairing lung tissue damage after viral infection, comprising the following components in parts by weight: 80 parts of yam powder, 80 parts of giant salamander small molecule peptide powder (the weight ratio of yam powder to giant salamander small molecule peptide powder is 1:1), 60 parts of kudzu root powder, 50 parts of lily powder, 40 parts of acerola cherry powder, 20 parts of yeast β-glucan, 20 parts of jujube powder, 10 parts of platycodon root extract, 10 parts of salvia miltiorrhiza extract, 10 parts of turmeric extract, 10 parts of natto freeze-dried powder, 8 parts of houttuynia cordata extract, and 5 parts of fucoidan.

[0026] Preparation method: S1 Raw material pretreatment: Yam powder, kudzu root powder, lily powder, acerola cherry powder, jujube powder and natto freeze-dried powder are dried at 50℃ to a moisture content of ≤8%; giant salamander small molecule peptide powder, platycodon root extract, salvia miltiorrhiza extract, turmeric extract and houttuynia cordata extract are processed through an 80-mesh sieve. S2 Plant Extract Premix: Platycodon grandiflorum extract, Salvia miltiorrhiza extract, Curcuma longa extract and Houttuynia cordata extract are added to a mixing device and mixed at 15 r / min for 15 minutes to obtain a plant extract mixture; S3 Basic Powder Mixing: Add the pretreated yam powder, kudzu root powder, lily powder, acerola cherry powder, jujube powder, natto freeze-dried powder, giant salamander small molecule peptide powder and S2 mixture to the mixing equipment and mix at 15 r / min for 30 minutes to obtain basic mixed powder. S4 Functional Ingredient Compound: Add yeast β-glucan and fucoidan, continue mixing for 30 minutes to obtain compound powder; S5 Formulation Processing: The uniformly mixed compound powder is metered, dispensed, and sealed according to predetermined specifications to obtain the powder product.

[0027] Example 2: Composition containing supplementary nutritional ingredients This embodiment further adds auxiliary nutritional components based on Example 1. The components include: 80 parts of yam powder, 80 parts of giant salamander small molecule peptide powder (the weight ratio of yam powder to giant salamander small molecule peptide powder is 1:1), 60 parts of kudzu root powder, 50 parts of lily powder, 40 parts of acerola cherry powder, 20 parts of yeast β-glucan, 20 parts of jujube powder, 10 parts of platycodon root extract, 10 parts of salvia miltiorrhiza extract, 10 parts of turmeric extract, 10 parts of natto freeze-dried powder, 8 parts of houttuynia cordata extract, 6 parts of zinc-enriched yeast powder, 5 parts of defatted flaxseed powder, 5 parts of ultrafine rice bran powder, and 5 parts of fucoidan.

[0028] Preparation method: S1 Raw Material Pretreatment: Zinc-enriched yeast powder, defatted flaxseed powder, ultrafine rice bran powder, and plant powders such as yam powder are dried together at 50℃ until the moisture content is ≤8%; Giant salamander small molecule peptide powder and various plant extracts are processed through an 80-mesh sieve. S2 Plant Extract Pre-dilution Mixture: Mix 10 parts of Platycodon grandiflorus extract, 10 parts of Salvia miltiorrhiza extract, 10 parts of Curcuma longa extract and 8 parts of Houttuynia cordata extract, add 19 parts of ultrafine rice bran powder with a particle size of 100 mesh (accounting for 50% of the total weight of the extract) as a porous adsorption carrier, and premix at 15 r / min for 15 minutes to obtain the plant extract pre-dilution mixture. S3 Basic Powder Mixing: Add the pretreated plant powder, zinc-enriched yeast powder, defatted flaxseed powder, the remaining ultrafine rice bran powder, giant salamander small molecule peptide powder and S2 mixture into the mixing equipment and mix for 30 minutes at a speed of 15 r / min. S4 Functional Ingredient Complex: Add yeast β-glucan and fucoidan and continue mixing for 30 minutes; S5 Formulation processing: Powder products are obtained by metering and packaging.

[0029] Example 3: Complete compound composition This embodiment provides a complete formulation composition comprising the following components by weight: 80 parts yam powder, 80 parts giant salamander small molecule peptide powder (the weight ratio of yam powder to giant salamander small molecule peptide powder is 1:1), 60 parts kudzu root powder, 50 parts lily powder, 40 parts acerola cherry powder, 20 parts yeast β-glucan, 20 parts jujube powder, 10 parts platycodon root extract, 10 parts salvia miltiorrhiza extract, 10 parts turmeric extract, 10 parts natto freeze-dried powder, 8 parts houttuynia cordata extract, 6 parts zinc-enriched yeast powder, 5 parts defatted flaxseed powder, 5 parts ultrafine rice bran powder, 5 parts fucoidan, 1 part ergothioneine, 1 part pyrroquinoline quinone disodium salt (PQQ), 1 part mogroside, and 0.5 parts natural vitamin D3 powder.

[0030] Preparation method: S1 Raw material pretreatment: Yam powder, kudzu root powder, lily powder, acerola cherry powder, jujube powder, natto freeze-dried powder, zinc-enriched yeast powder, defatted flaxseed powder, and ultrafine rice bran powder are dried at 50℃ to a moisture content of ≤8%; giant salamander small molecule peptide powder and various extracts are sieved through an 80-mesh sieve. S2 Pre-dilution and mixing of plant extracts: Mix 10 parts of Platycodon grandiflorus extract, 10 parts of Salvia miltiorrhiza extract, 10 parts of Curcuma longa extract and 8 parts of Houttuynia cordata extract, add 28.5 parts of ultrafine rice bran powder with a particle size of 100 mesh (accounting for 75% of the total weight of the extracts) as a porous adsorption carrier, and premix at 15 r / min for 15 minutes to obtain the pre-dilution mixture of plant extracts. S3 Basic Powder Mixing: Add all the plant matrix powders pretreated in S1, the giant salamander small molecule peptide powder, and the S2 premix to the mixing equipment and mix at 15 r / min for 30 minutes to obtain the basic mixed powder. S4 Functional Ingredient Compounding: Add yeast β-glucan, fucoidan, ergothionein, disodium pyrroquinone (PQQ), mogroside and natural vitamin D3 powder to the basic mixed powder, and continue mixing for 30 minutes to obtain the compound powder composition. S5 Formulation processing: metered packaging (divided into 5.025g or 5g portions per strip) yields the powder product.

[0031] Example 4: Evaluation of powder flowability, mixing uniformity and flavor masking effect Experimental Groups: Example 3 of this invention: prepared using the method of Example 3 (using small molecule peptide powder of giant salamander with a D50 of 15.2 μm, and using 100-mesh ultrafine rice bran powder as a carrier in step S2); Comparative Example 1 (Traditional Direct Mixing Group): The same formulation as in Example 3 was used, but the small molecule peptide powder of giant salamander was not controlled for particle size (D50>80 μm) and was not pre-diluted in S2. All raw materials were directly dry mixed for 30 minutes. Comparative Example 2 (Conventional Carrier Replacement Group): The same formula and steps as in Example 3 were used, but the "ultrafine rice bran powder" in step S2 was replaced with an equal mass of ordinary food-grade maltodextrin (100 mesh) as the carrier.

[0032] Test method: Particle size determination: The median particle size D50 of each raw material powder was determined using a laser particle size analyzer; Flowability (angle of repose): The fixed funnel method was used to determine the flowability (the powder was put into the funnel and allowed to flow down and accumulate on a flat plate. The angle between the inclined plane of the cone and the horizontal plane was measured). Each sample was measured 3 times and the average value was taken. Bulk density: Loose packing density and tapped density were measured separately; Mixing homogeneity (CV%): Random multi-point sampling was used to determine the content of the index component curcumin by high performance liquid chromatography (HPLC), and the coefficient of variation (CV%) was calculated. The HPLC detection conditions were as follows: C18 column (4.6 mm × 250 mm, 5 μm), acetonitrile-0.1% phosphoric acid aqueous solution (55:45) as mobile phase, detection wavelength 425 nm, flow rate 1.0 mL / min, column temperature 30 ℃; Vibration stratification experiment: Each group of powders was placed in a sealed container and placed on a vibration platform to vibrate at a frequency of 50 Hz for 10 minutes. Then, samples were taken from the top, middle and bottom layers to determine the curcumin content and the relative standard deviation (RSD%) of the content of each layer was calculated.

[0033] Flavor rating: Ten sensory-trained evaluators will give a comprehensive sensory score (1-10 points, with lower scores indicating a more uniform aroma and weaker local irritation) on the characteristic aroma of the compound powder.

[0034] Table 1. Comparison of physical properties and mixing uniformity of composite powders prepared by different processes. Example 3 32.1° 3.20% 2.80% Comparative Example 1 (Traditional Direct Hybrid Group) 41.5° 12.60% 15.40% Comparative Example 2 (Conventional Vector Group) 37.8° 8.50% 9.60% The above experimental results are the average of three parallel experiments. Result analysis: Flowability Evaluation: As shown in Table 1, the angle of repose of Comparative Example 1 (traditional direct mixing group) was 41.5°, indicating poor powder flowability; the angle of repose of Comparative Example 2 (conventional carrier replacement group) was 37.8°, showing a slight decrease but with limited improvement; while the angle of repose of Example 3 of this invention was 32.1°, significantly smaller than the two comparative examples, indicating that the powder flowability was significantly improved after particle size distribution control. The results of loose density and tapped density measurements also showed a consistent trend. The bulk density of Example 3 of this invention was higher than that of the two comparative examples, confirming that the compact packing structure formed by small-diameter peptide powder filling the gaps between large particles is beneficial to improving the flowability and filling performance of the powder.

[0035] Evaluation of mixing uniformity: The content of curcumin in each group of powders was determined by HPLC and the coefficient of variation (CV%) was calculated. The CV value of Comparative Example 1 was 12.6%, indicating that the traditional direct dry mixing method is difficult to achieve uniform dispersion of trace extracts. Comparative Example 2 used maltodextrin as a carrier for premixing, and the CV value was 8.5%, which was lower but still relatively high. The CV value of Example 3 of this invention was 3.2%, which was significantly better than the two comparative examples. This shows that using the natural cellulose network structure of ultrafine rice bran powder to pre-dilute and disperse multi-source extracts can effectively avoid local aggregation of trace components and achieve a highly uniform mixing effect.

[0036] The vibration stratification test results showed that after vibration treatment, the RSD of curcumin content in the upper, middle and lower layers of Comparative Example 1 was 15.4%, indicating that the powder was prone to stratification under transportation vibration conditions; the RSD of Comparative Example 2 was 9.6%, which was somewhat improved but still quite obvious; the RSD of Example 3 of the present invention was 2.8%, and the content difference between each layer was very small, indicating that the close packing structure formed by particle size distribution has good anti-stratification ability.

[0037] Flavor uniformity evaluation: Sensory evaluation results showed that Comparative Example 1 had the highest score due to the local enrichment of trace extracts, resulting in a concentrated and highly pungent characteristic odor; Comparative Example 2 had no significant odor improvement effect due to the limited adsorption capacity of maltodextrin; Example 3 of this invention, through the adsorption and isolation effect of the porous carrier, effectively reduced the local enrichment of characteristic odor components, resulting in the most uniform odor distribution and the lowest score. This indicates that the carrier pre-dilution dispersion process of this invention can effectively improve the flavor consistency of the product.

[0038] The above results indicate that by controlling the particle size of small molecule peptides and the gradation of plant powders, combined with the pre-dilution and dispersion effect of ultrafine rice bran powder as a porous carrier, the present invention can significantly improve the flowability, mixing uniformity, anti-stratification ability and flavor consistency of composite powders.

[0039] Example 5: Observation on the effects of the composition on relevant physiological indicators of the body Experimental objective: This experiment aims to preliminarily observe the influence trend of the composition of the present invention on the physiological indicators related to the nutritional status of the body during the recovery period after viral infection, and to provide a reference for the composition as a dietary nutritional supplement during the recovery period.

[0040] Experimental Materials and Methods: Experimental animals: C57BL / 6 mice; Model establishment: A short-term metabolic stress model induced by Poly(I:C) was used to simulate the metabolic consumption state of the body after viral infection; Randomized groups: normal control group, model group, low-dose group of Example 3 (200 mg / kg·bw), and high-dose group of Example 3 (600 mg / kg·bw); Intervention period: 7 consecutive days; Detection indicators: (1) Weight recovery rate: the ratio of weight after intervention to the lowest weight after modeling; (2) Average daily food intake; (3) Serum total protein (TP); (4) Serum albumin (ALB); (5) Serum blood urea nitrogen (BUN); (6) Serum superoxide dismutase (SOD) activity.

[0041] The indicators tested in this experiment are all related to nutritional status and do not involve the evaluation of the degree of lung tissue lesions or damage.

[0042] Experimental results: Compared with the model group, the mice treated with the composition of this invention showed improved weight recovery rate and daily food intake, indicating that the composition has a certain degree of improvement effect on feeding ability and weight recovery during the recovery period. Serum total protein and albumin levels showed an increasing trend, while serum urea nitrogen levels showed a decreasing trend, indicating that the protein, peptides and polysaccharide nutrients provided by the composition can be effectively utilized by the body, which helps to improve the nitrogen balance during the recovery period. At the same time, serum SOD activity showed an increasing trend, indicating that the composition of this invention has an improving effect on the antioxidant nutritional status of the body during the recovery period. The changing trends of the above indicators became more obvious with increasing dosage. This experiment is only used to illustrate the nutritional support observation of the composition as a dietary supplement and is not intended to prove that it has a direct therapeutic effect on viruses or tissue lesions.

[0043] Example 6: Storage stability of the composition and granule processing To evaluate the physical and functional stability of the composition of the present invention during storage, the composite composition powder prepared in Example 3 was sealed in aluminum foil bags and stored in a constant temperature and humidity environment of 25℃±2℃ and 60%±5% for storage tests. Samples were taken and tested at 0 months, 1 month, 3 months and 6 months of storage.

[0044] The testing items include: (1) the appearance of the product (whether there is moisture absorption, clumping, or delamination); (2) Moisture content (determined according to GB 5009.3); (3) Water activity (Aw); (4) Powder flowability (evaluated by change in angle of repose); (5) Uniformity of characteristic component content (CV value of curcumin content).

[0045] Experimental results: After 6 months of storage In terms of appearance, the powder remained loose throughout the entire 6-month storage period, without any moisture absorption, clumping, or visible stratification, indicating that the particle size distribution of the composition has good anti-caking ability.

[0046] Regarding moisture content and water activity, the product's moisture content and water activity remained at low levels during storage, meeting the quality control requirements for solid powder products. This indicates that the migration of powder moisture can be effectively controlled through raw material pretreatment drying and reasonable packaging barriers.

[0047] In terms of flowability, the angle of repose of the powder did not change significantly before and after storage, and the flowability remained good, further confirming the stability of the particle size distribution structure under long-term storage conditions.

[0048] Regarding the uniformity of content, the CV value of curcumin content did not change significantly during storage, indicating that the dispersion of trace components in the composite powder remained stable and there was no migration or reaggregation.

[0049] The above results indicate that the present invention helps to reduce direct contact and agglomeration sites between small particles by (1) controlling the particle size distribution between small-sized giant salamander peptide powder (D50≈15μm) and larger-sized plant powder (D50≈80-90μm); (2) using ultrafine rice bran powder as a carrier to pre-dilute and disperse low-added plant extracts, which helps to avoid local aggregation of trace components in the mixed system; and (3) using a phased step-by-step mixing process, which can reduce the uneven mixing caused by the interface difference between materials to a certain extent. The above synergistic effect helps to improve the physical stability of the composite powder system and enable the product to maintain good quality consistency during the shelf life.

[0050] Granule preparation: Take the composite composition powder obtained in step S4 of Example 3, add 10% of the total weight of the composite powder in 85% ethanol solution as a wetting agent, granulate through a 20-mesh sieve, dry at 50°C until the moisture content is ≤5%, and obtain granule product after granulation. The granules have good flowability, disperse rapidly after brewing, and have a suitable taste.

[0051] The present invention and its embodiments have been described above. This description is not restrictive, and the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and, without departing from the spirit of the invention, design similar structures and embodiments without creative effort, all such designs should fall within the protection scope of the present invention.

Claims

1. A composition for repairing lung tissue damage after viral infection, characterized in that: The mixture comprises 40-120 parts of yam powder, 40-120 parts of giant salamander small molecule peptide powder, 30-90 parts of kudzu root powder, 25-80 parts of lily powder, 20-60 parts of acerola cherry powder, 10-40 parts of yeast β-glucan, 10-40 parts of jujube powder, 5-20 parts of platycodon root extract, 5-20 parts of salvia miltiorrhiza extract, 5-20 parts of turmeric extract, 5-20 parts of natto freeze-dried powder, 4-15 parts of houttuynia cordata extract, and 2-10 parts of fucoidan; wherein the average molecular weight of the giant salamander small molecule peptide powder is less than 1000 Daltons.

2. The composition for repairing lung tissue damage after viral infection according to claim 1, characterized in that: The weight ratio of yam powder to giant salamander small molecule peptide powder is 1:0.33 to 1:3, preferably 1:0.5 to 1:1.5, and most preferably 1:

1. The median particle size D50 of the giant salamander small molecule peptide powder is 12.5 to 18.2 μm, and the median particle size D50 of the yam powder and kudzu root powder is 75 to 110 μm.

3. The composition for repairing lung tissue damage after viral infection according to claim 2, characterized in that: It also includes auxiliary nutritional components: 2-10 parts zinc-enriched yeast powder, 2-10 parts defatted flaxseed powder, and 2-10 parts ultrafine rice bran powder.

4. The composition for repairing lung tissue damage after viral infection according to claim 3, characterized in that: It also includes antioxidant and mitochondrial nutrients: 0.5–3 parts of ergothioneine and 0.2–2 parts of disodium pyrroloquinoline quinone.

5. The composition for repairing lung tissue damage after viral infection according to claim 4, characterized in that: It also includes 0.5 to 3 parts of mogroside and 0.1 to 1 part of natural vitamin D3 powder.

6. The composition for repairing lung tissue damage after viral infection according to claim 5, characterized in that: 80 parts yam powder, 80 parts giant salamander small molecule peptide powder, 60 parts kudzu root powder, 50 parts lily powder, 40 parts acerola cherry powder, 20 parts yeast β-glucan, 20 parts jujube powder, 10 parts platycodon root extract, 10 parts salvia miltiorrhiza extract, 10 parts turmeric extract, 10 parts natto freeze-dried powder, 8 parts houttuynia cordata extract, 6 parts zinc-rich yeast powder, 5 parts defatted flaxseed powder, 5 parts ultrafine rice bran powder, 5 parts fucoidan, 1 part ergothioneine, 1 part disodium pyrrolizidine quinoline quinone, 1 part mogroside, and 0.5 parts natural vitamin D3 powder.

7. A method for preparing a composition for repairing lung tissue damage after viral infection according to claims 1-6, characterized in that: S1 Raw Material Pretreatment: Yam powder, kudzu root powder, lily powder, acerola cherry powder, jujube powder, natto freeze-dried powder, defatted flaxseed powder, and ultrafine rice bran powder are dried at a temperature of 40-70℃ to ensure that the moisture content of the raw materials is ≤8%; Giant salamander small molecule peptide powder and plant extracts are processed through a 60-100 mesh sieve. S2 Plant Extract Pre-dilution Mixing: Platycodon grandiflorus extract, Salvia miltiorrhiza extract, Curcuma longa extract and Houttuynia cordata extract are mixed according to the formula, and porous carrier powder accounting for 50% to 100% of the total weight of the extracts is added, that is, the mass ratio of plant extracts to porous carrier powder is 1:0.5 to 1:

1. The porous carrier powder is ultrafine rice bran powder or defatted flaxseed powder with an average particle size of 80 to 200 mesh. The mixture is premixed at a speed of 10 to 20 r / min for 10 to 20 minutes to obtain the plant extract pre-dilution mixture. S3 Basic Powder Mixing: Add the pretreated plant powder raw materials, giant salamander small molecule peptide powder and the pre-diluted mixture of plant extracts obtained in step S2 to the mixing equipment and mix at a speed of 8-20 r / min for 15-40 minutes to obtain the basic mixed powder. S4 Functional Component Compound: Add yeast β-glucan and fucoidan to the basic mixed powder, and continue mixing for 15-40 minutes to obtain the compound powder; S5 Formulation: The composite powder is prepared into powder, granules, capsules or tablets.

8. The use of the composition for repairing lung tissue damage after viral infection according to any one of claims 1-6 in the preparation of dietary nutritional supplements for the recovery stage, characterized in that: The dietary supplement products mentioned are functional foods, dietary supplements, or special dietary foods.