A nano-peptide collagen mask with wrinkle-removing and fine line-fading effects and a preparation method thereof

Abstract

The application provides a kind of nanopeptide collagen protein mask with the effect of removing wrinkles and light lines and a preparation method thereof, and relates to the technical field of cosmetics.According to weight parts, it comprises 0.1-10 parts of nanopeptide liposome, 1-15 parts of recombinant collagen, 0.1-5 parts of ceramide, and 0.05-3 parts of hyaluronic acid.The nanopeptide liposome of the application significantly improves the transdermal absorption rate and stability of the peptide, encapsulates acetyl hexapeptide-8 and palmitoyl tripeptide-1 in the nanoliposome, the phospholipid bilayer is similar to the lipid structure of the stratum corneum, easily fuses and penetrates the stratum corneum, and delivers the peptide to the deep layer of the epidermis or even the superficial layer of the dermis.Meanwhile, the liposome encapsulation can isolate external oxygen, significantly improving the stability of the peptide during storage and use.The application does not contain irritating chemical components, is suitable for various skin types including sensitive skin, and has good safety.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects and its preparation method. Background Technology

[0002] As the surface through which the human body comes into contact with the external environment, the skin plays a fundamental role in maintaining the body's physiological balance and protecting against external aggressors. Skin tissue, from the inside out, consists of the subcutaneous tissue, dermis, and epidermis. Fibroblasts in the dermis are specifically responsible for synthesizing extracellular matrix components such as elastic fibers and collagen. With age, the number and activity of fibroblasts gradually decline, leading to a weakening of the dermal structure's support, manifested as a loss of elasticity and tension, and skin laxity. This makes the skin unable to effectively resist the mechanical stress generated by muscle contraction, ultimately causing thinning and the formation of visible wrinkles.

[0003] To slow down the aging process of the skin, a variety of preventative measures and skincare strategies can be adopted, including using skincare products containing antioxidants, performing regular skincare, avoiding UV exposure, maintaining a healthy lifestyle, and addressing skin problems promptly.

[0004] There are many anti-wrinkle and wrinkle-reducing skincare products on the market. Peptide ingredients (such as palmitoyl tripeptide-1 and acetyl hexapeptide-8) are currently the most widely used active ingredients in anti-aging skincare products. They exert their anti-wrinkle effects by stimulating collagen regeneration or inhibiting neurotransmitter release. However, peptide molecules are easily degraded and inactivated in formulations and on the skin surface. Moreover, most peptides are water-soluble, and their ability to penetrate the lipid barrier of the stratum corneum is limited, resulting in a low dose reaching the target site and affecting efficacy. Collagen is another commonly used anti-aging ingredient, but natural collagen has a large molecular weight, making it difficult to penetrate the skin when applied topically. It mainly stays on the skin surface to moisturize, but cannot effectively replenish collagen lost in the dermis. Simply combining peptides and collagen can cover both the "promoting collagen production" and "replenishing collagen" pathways, but it does not solve the problems of peptides' difficulty in penetrating the skin and their poor stability. Whether the combination can produce a synergistic effect is also lacking verification.

[0005] Therefore, providing a product with high transdermal peptide absorption rate, good stability, and the ability to simultaneously achieve immediate wrinkle removal and long-lasting anti-aging is a technical problem that needs to be solved in this field. Summary of the Invention

[0006] Therefore, this invention proposes a nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects and its preparation method.

[0007] The technical solution of this invention is implemented as follows: A nanopeptide collagen patch with wrinkle-reducing and fine line-fading effects comprises the following raw materials by weight: 0.1-10 parts nanopeptide liposomes, 1-15 parts recombinant collagen, 0.1-5 parts ceramide, and 0.05-3 parts hyaluronic acid. The nanopeptide liposomes encapsulate acetyl hexapeptide-8 and palmitoyl tripeptide-1, with an average particle size of 50-300 nm.

[0008] Furthermore, the preparation method of the nanopeptide liposomes includes: (1) Add acetyl hexapeptide-8 and palmitoyl tripeptide-1 to PBS buffer to form an inner aqueous phase; (2) Phospholipids and cholesterol are added to an ethanol-ether mixed solvent to form an organic phase; (3) Inject the aqueous phase into the organic phase, ultrasonically emulsify, and remove the organic solvent by rotary evaporation; (4) The nanopeptide liposomes are obtained by extruding and granulating through a microporous filter membrane.

[0009] Further, in step (1), the mass ratio of acetyl hexapeptide-8 to palmitoyl tripeptide-1 is 1:1-3; the concentration of acetyl hexapeptide-8 in the inner aqueous phase is 0.1-0.4 g / L; and the pH value of the PBS buffer is 6.8-7.2.

[0010] Furthermore, in step (2), the molar ratio of phospholipids to cholesterol is 3-8:1; the volume ratio of ethanol to diethyl ether in the ethanol-diethyl ether mixed solvent is 1:3-4; and the concentration of phospholipids in the organic phase is 15-30 mg / mL.

[0011] Furthermore, in step (3), the volume ratio of the internal aqueous phase to the organic phase is 1:3-5; the ultrasonic emulsification is performed under the conditions of 20-26kHz, 100-200W, and 0-4℃ ice bath, using pulse mode, working for 2-5s, with an interval of 2-5s, and a total ultrasonic time of 3-5min.

[0012] Furthermore, in step (4), the average particle size of the nanopeptide liposomes is 80-200 nm.

[0013] Furthermore, it also includes the following ingredients: 0.05-3 parts ectoine, 0.1-5 parts arbutin, 0.1-3 parts vitamin E, 0.05-2 parts carnosine, and 0.05-3 parts palmitoyl tripeptide-5.

[0014] A method for preparing a nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects, the specific preparation steps of which include: S1. Add recombinant collagen, ceramide, hyaluronic acid, ectoine, arbutin, vitamin E, carnosine and palmitoyl tripeptide-5 to water, add nanopeptide liposomes, mix evenly to obtain coating solution. S2. Apply the coating liquid to the substrate, dry it, cover it with an isolation layer, cut it into shape, and obtain the nano-peptide collagen film.

[0015] Furthermore, in step S1, the total solids content in the coating liquid is 10%-15%.

[0016] Furthermore, in step S2, the substrate is silk fiber or non-woven fabric; the drying is carried out at 30-35°C until the moisture content is 15wt%-20wt%.

[0017] Compared with the prior art, the beneficial effects of the present invention are: 1. The nanopeptide liposomes of this invention significantly improve the transdermal absorption rate and stability of peptides. Acetyl hexapeptide-8 and palmitoyl tripeptide-1 are encapsulated within nanoliposomes. The phospholipid bilayer has a structure similar to the lipid structure of the stratum corneum, facilitating fusion and penetration into the stratum corneum, delivering peptides to the deep epidermis and even the superficial dermis. Simultaneously, liposome encapsulation isolates the peptides from external oxygen, significantly improving their stability during storage and use. The coating solution of this invention is dried to a moisture content of 15wt%-20wt%, allowing the nanopeptide liposomes to maintain their vesicle structure and high encapsulation retention rate.

[0018] 2. The acetyl hexapeptide-8 of this invention relaxes facial muscles and reduces dynamic wrinkles immediately by inhibiting the release of neurotransmitters at the neuromuscular junction, resulting in rapid onset of action. Palmitoyl tripeptide-1, as a signal peptide, continuously stimulates fibroblasts to synthesize endogenous collagen, providing a long-lasting anti-aging effect. Recombinant collagen provides exogenous collagen supplementation, forming a moisturizing film on the skin surface while penetrating into the epidermis to exert immediate filling and repair effects. These three components work synergistically to achieve comprehensive anti-aging effects that are immediate, sustained, and provide long-term improvement.

[0019] 3. The ceramide of this invention replenishes intercellular lipids in the stratum corneum, repairs the skin barrier function, and reduces transepidermal water loss; hyaluronic acid forms a highly water-content viscoelastic film on the skin surface, synergistically locking in the active ingredients in the nanoliposomes with the ceramide, achieving a sustained-release effect and preventing the loss of active substances in a short time. The synergistic effect of ceramide and hyaluronic acid prolongs the duration of action of the active ingredients.

[0020] 4. This invention contains no irritating chemical components, and the nanoliposome carrier is a biocompatible material. The recombinant collagen and hyaluronic acid have low sensitization potential, making them suitable for all skin types, including sensitive skin, and exhibiting good safety. The prepared patch formulation is convenient to use, has a large drug loading capacity, and facilitates continuous nighttime repair. Detailed Implementation

[0021] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0022] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0023] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0024] The recombinant collagen of this invention has a molecular weight of 10-100 kDa.

[0025] Example 1 A nanopeptide collagen patch with wrinkle-reducing and fine line-fading effects comprises the following ingredients by weight: 5 parts nanopeptide liposomes, 10 parts recombinant collagen, 3 parts ceramide, 1 part hyaluronic acid, 1 part ectoine, 2 parts arbutin, 2 parts vitamin E, 1 part carnosine, and 1 part palmitoyl tripeptide-5.

[0026] The preparation methods of nanopeptide liposomes include: (1) Acetyl hexapeptide-8 and palmitoyl tripeptide-1 in a mass ratio of 1:2 were added to PBS buffer (pH=7.0±0.1) to form an inner aqueous phase, in which the concentration of acetyl hexapeptide-8 was 0.2 g / L; (2) Phospholipids and cholesterol in a molar ratio of 5:1 were added to an ethanol-ether mixed solvent (ethanol and ether volume ratio of 1:3.5) to form an organic phase, the concentration of phospholipids in the organic phase being 20 mg / mL; (3) The aqueous phase was injected into the organic phase at a volume ratio of 1:4. Under the conditions of 23kHz, 150W, and 2℃ ice bath, the pulse mode was used, with a working time of 3s and an intermittent time of 3s. The total ultrasonic time was 4min. The organic solvent was removed by rotary evaporation. (4) The granules were extruded through a microporous filter membrane to obtain nanopeptide liposomes with an average particle size of 150 nm.

[0027] The preparation method of the above-mentioned nanopeptide collagen patch includes the following specific steps: S1. Add recombinant collagen, ceramide, hyaluronic acid, ectoine, arbutin, vitamin E, carnosine, and palmitoyl tripeptide-5 to water, add nanopeptide liposomes, mix evenly to obtain a coating solution with a total solids content of 13%. S2. Coat the nonwoven fabric with the coating liquid, dry it at 35°C until the moisture content is 15wt%, cover it with the isolation layer, cut it into shape, and obtain the nano peptide collagen film.

[0028] Test Example 1 The changes in liposome particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency of the nanopeptide liposomes in Example 1 were tested after drying at 35°C to a water content of 15 wt%.

[0029] Experimental method: The sample of nanopeptide liposomes before drying was used as the control group. In Example 1, the sample of nanopeptide liposomes dried at 35°C to a water content of 15wt% was added to ultrapure water for rehydration. The volume was the same as that of the sample of nanopeptide liposomes before drying. The sample was gently vortexed for 1 min until it was uniformly dispersed to obtain rehydrated nanopeptide liposomes. Take 50 μL of the control group (before drying) and the rehydrated nanopeptide liposome sample, dilute them 100 times with ultrapure water, inject them into quartz cuvettes, and use a Malvern Zetasizer Nano ZS dynamic light scattering instrument at 25℃, 173° scattering angle and equilibration time of 120 s to measure the Z-average particle size and polydispersity index (PDI) of each group. Each group was measured in triplicate.

[0030] Take the diluted samples and use the same electrophoresis light scattering module to measure the Zeta potential at 25℃. Each group was measured in parallel three times.

[0031] The encapsulation efficiency of the control group and the rehydrated nanopeptide liposome samples was determined by ultrafiltration centrifugation.

[0032] The results are shown in Table 1.

[0033] Table 1

[0034] As can be seen from Table 1, the nanopeptide liposomes of the present invention remain monodisperse during the drying process, the liposome vesicles do not fuse, the zeta potential changes very little, the surface charge of the vesicles is stable, and the membrane structure remains intact; the encapsulation efficiency and retention rate do not change much, and no significant leakage occurs between acetyl hexapeptide-8 and palmitoyl tripeptide-1 before and after drying, indicating that the nanopeptide liposomes of the present invention can maintain the integrity of the vesicle structure when dried at 35°C to a water content of 15wt%.

[0035] Example 2 A nanopeptide collagen patch with wrinkle-reducing and fine line-fading effects comprises the following ingredients by weight: 0.1 parts nanopeptide liposomes, 1 part recombinant collagen, 0.1 parts ceramide, 0.05 parts hyaluronic acid, 0.05 parts ectoine, 0.1 parts arbutin, 0.1 parts vitamin E, 0.05 parts carnosine, and 0.05 parts palmitoyl tripeptide-5.

[0036] The preparation methods of nanopeptide liposomes include: (1) Acetyl hexapeptide-8 and palmitoyl tripeptide-1 in a mass ratio of 1:1 were added to PBS buffer (pH=7.0±0.1) to form an inner aqueous phase, in which the concentration of acetyl hexapeptide-8 was 0.1 g / L; (2) Phospholipids and cholesterol in a molar ratio of 3:1 were added to an ethanol-ether mixed solvent (ethanol and ether in a volume ratio of 1:3) to form an organic phase, wherein the concentration of phospholipids in the organic phase was 15 mg / mL. (3) The aqueous phase was injected into the organic phase at a volume ratio of 1:3. Under the conditions of 20kHz, 100W, and 0℃ ice bath, the pulse mode was used, with a working time of 2s and an interval of 2s, and the total ultrasonic time was 3min. The organic solvent was removed by rotary evaporation. (4) The granules were extruded through a microporous filter membrane to obtain nanopeptide liposomes with an average particle size of 80 nm.

[0037] The preparation method of the above-mentioned nanopeptide collagen patch includes the following specific steps: S1. Add recombinant collagen, ceramide, hyaluronic acid, ectoine, arbutin, vitamin E, carnosine, and palmitoyl tripeptide-5 to water, add nanopeptide liposomes, mix evenly to obtain a coating solution with a total solids content of 10%. S2. Coat the nonwoven fabric with the coating liquid, dry it at 35°C until the moisture content is 15wt%, cover it with the isolation layer, cut it into shape, and obtain the nano peptide collagen film.

[0038] Example 3 A nanopeptide collagen patch with wrinkle-reducing and fine line-fading effects comprises the following ingredients by weight: 10 parts nanopeptide liposomes, 15 parts recombinant collagen, 5 parts ceramide, 3 parts hyaluronic acid, 3 parts ectoine, 5 parts arbutin, 3 parts vitamin E, 2 parts carnosine, and 3 parts palmitoyl tripeptide-5.

[0039] The preparation methods of nanopeptide liposomes include: (1) Acetyl hexapeptide-8 and palmitoyl tripeptide-1 in a mass ratio of 1:3 were added to PBS buffer (pH=7.0±0.1) to form an inner aqueous phase, in which the concentration of acetyl hexapeptide-8 was 0.4 g / L; (2) Phospholipids and cholesterol in a molar ratio of 8:1 were added to an ethanol-ether mixed solvent (ethanol and ether volume ratio of 1:4) to form an organic phase, in which the concentration of phospholipids was 30 mg / mL. (3) The aqueous phase was injected into the organic phase at a volume ratio of 1:5. Under the conditions of 26kHz, 200W, and 4℃ ice bath, the pulse mode was used, with a working time of 5s and an intermittent time of 5s. The total ultrasonic time was 5min. The organic solvent was removed by rotary evaporation. (4) The granules were extruded through a microporous filter membrane to obtain nanopeptide liposomes with an average particle size of 200 nm.

[0040] The preparation method of the above-mentioned nanopeptide collagen film includes the following specific steps: S1. Add recombinant collagen, ceramide, hyaluronic acid, ectoine, arbutin, vitamin E, carnosine, and palmitoyl tripeptide-5 to water, add nanopeptide liposomes, mix evenly to obtain a coating solution with a total solids content of 15%. S2. Coat the nonwoven fabric with the coating liquid, dry it at 35°C until the moisture content is 15wt%, cover it with the isolation layer, cut it into shape, and obtain the nano peptide collagen film.

[0041] Comparative Example 1 The difference from Example 1 is that the nanopeptide liposomes are replaced with acetyl hexapeptide-8 and palmitoyl tripeptide-1 in a mass ratio of 1:2, otherwise the same as in Example 1.

[0042] Comparative Example 2 The difference from Example 1 is that palmitoyl tripeptide-1 is missing, and the nanopeptide liposomes only encapsulate acetyl hexapeptide-8; otherwise, they are the same as in Example 1.

[0043] Comparative Example 3 The difference from Example 1 is that acetyl hexapeptide-8 is missing, and the nanopeptide liposomes only encapsulate palmitoyl tripeptide-1; otherwise, they are the same as in Example 1.

[0044] Comparative Example 4 The difference from Example 1 is that it lacks recombinant collagen, but otherwise it is the same as Example 1.

[0045] Comparative Example 5 The difference from Example 1 is that it lacks ceramide and hyaluronic acid, but otherwise it is the same as Example 1.

[0046] Test Example 2 The coating solutions from Examples 1-3 and Comparative Examples 1-5 were tested for their ability to inhibit matrix metalloproteinases.

[0047] Matrix metalloproteinases (MMPs) are a class of proteolytic enzymes that degrade collagen, elastin, and proteoglycans. MMP1 primarily degrades type I collagen. When exposed to ultraviolet radiation, the expression level of MMP1 increases, leading to increased abnormal collagen degradation, reduced skin strength and elasticity, skin aging, and the appearance of wrinkles.

[0048] Experimental method: Human skin fibroblasts in good exponential growth phase were taken and digested with 0.25% trypsin digestion solution. The cells were counted at a rate of 1×10⁻⁶. 6Cells / mL were prepared into a cell suspension, and the cell suspension was appropriately diluted to 1×10⁻⁶. 5 Cell suspension was seeded into 6-well plates, and a UV photoaging model was established when the cells reached 80% confluence.

[0049] Blank control group: 200 μL of PBS was added, and the culture medium was supplemented to 800 μL. No UV irradiation was performed. UV damage group: subjected to UV radiation and given PBS; Experimental group: Coating solutions obtained from Examples 1-3 and Comparative Examples 1-5 (2wt%); Wash the UV-damaged group and the experimental group repeatedly with an appropriate amount of PBS. Add 200 μL of PBS to each well and irradiate under a UV lamp (80 mJ / cm²). 2 (The distance between the lamp source and the culture bottle is 15 cm). After irradiation, the PBS is discarded. The UV damage group is given PBS solution and culture medium to a final volume of 800 μL, and the experimental group is given culture medium and the sample to be tested to a final volume of 800 μL. The blank control group, UV damage group, and experimental group are incubated in the incubator for another 48 h. After incubation, scrape off cells, mix thoroughly by pipetting, extract cell proteins using RIPA, quantify BCA to 1 mg / mL, and perform the ELISA procedure according to the matrix metalloproteinase 1 instructions. Measure the absorbance (OD) value of each well at 450 nm and complete the readings within 15 minutes.

[0050] The experimental results are shown in Table 2.

[0051] Table 2

[0052] As can be seen from Table 2, the coating solutions of Examples 1-3 of the present invention have the effect of inhibiting the expression level of matrix metalloproteinase 1, which can prevent the degradation of type I collagen, prevent the skin from losing elasticity, and achieve anti-aging and firming effects.

[0053] Test Example 3 The anti-wrinkle effect of the coating liquids in Examples 1-3 and Comparative Examples 1-5 was tested.

[0054] Specific method: Mouse C2C12 myoblasts were seeded onto gelatin-coated plates and placed in culture medium (containing 2 / 3 MEM and 1 / 3 M199, 2 mmol / L L-glutamine, 100 IU / mL penicillin, 100 μg / mL streptomycin, and 5% fetal bovine serum) and cultured at 37°C in a 5% CO2 incubator until a monolayer of myofibrils formed. Then, spinal cord explants from 10-day-old mouse embryos with dorsal root ganglia were placed on the monolayer of myofibrils formed by myoblasts. After 48 hours of co-culture, neurites growing from the explants were observed to contact the myoblasts, and contraction began after 100 hours. After 4 weeks of co-culture, the nerve and muscle had fully connected, forming a fully differentiated striated muscle fiber model with a fully mature neuromuscular junction (equivalent to having a motor endplate). At this stage, the myofibrils could contract regularly, indicating that the culture model had been successfully established.

[0055] First, observe the muscle contraction frequency of the cultured model under a microscope for 30 seconds, repeat 5 times, count the results, and take the average value. Next, dilute the coating solutions from Examples 1-3 and Comparative Examples 1-5 with culture medium at a ratio of 1:100, and add them to the cultured model at a ratio of 5% (v / v). A blank control group is also set up with sterile water applied to the muscle. Observe the muscle contraction frequency for 30 seconds at 1 min, 2 h, and 6 h, and record the results. Each test is repeated 5 times.

[0056] The results are shown in Table 3.

[0057] Table 3

[0058] As can be seen from Table 3, compared with the blank control group, the number of muscle contractions decreased significantly after treatment with the coating solution of Examples 1-3, which showed a better anti-wrinkle and wrinkle-reducing effect.

[0059] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects, characterized in that, By weight, it includes the following ingredients: 0.1-10 parts nanopeptide liposomes, 1-15 parts recombinant collagen, 0.1-5 parts ceramide, and 0.05-3 parts hyaluronic acid; The nanopeptide liposomes encapsulate acetyl hexapeptide-8 and palmitoyl tripeptide-1, with an average particle size of 50-300 nm.

2. The nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects as described in claim 1, characterized in that, The preparation method of the nanopeptide liposomes includes: (1) Add acetyl hexapeptide-8 and palmitoyl tripeptide-1 to PBS buffer to form an inner aqueous phase; (2) Phospholipids and cholesterol are added to an ethanol-ether mixed solvent to form an organic phase; (3) Inject the aqueous phase into the organic phase, ultrasonically emulsify, and remove the organic solvent by rotary evaporation; (4) The nanopeptide liposomes are obtained by extruding and granulating through a microporous filter membrane.

3. The nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects as described in claim 2, characterized in that, In step (1), the mass ratio of acetyl hexapeptide-8 to palmitoyl tripeptide-1 is 1:1-3; the concentration of acetyl hexapeptide-8 in the inner aqueous phase is 0.1-0.4 g / L; and the pH value of the PBS buffer is 6.8-7.

2.

4. The nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects as described in claim 2, characterized in that, In step (2), the molar ratio of phospholipids to cholesterol is 3-8:1; the volume ratio of ethanol to diethyl ether in the ethanol-diethyl ether mixed solvent is 1:3-4; and the concentration of phospholipids in the organic phase is 15-30 mg / mL.

5. The nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects as described in claim 2, characterized in that, In step (3), the volume ratio of the internal aqueous phase to the organic phase is 1:3-5; the ultrasonic emulsification is carried out under the conditions of 20-26kHz, 100-200W, and 0-4℃ ice bath, using pulse mode, working for 2-5s, with an interval of 2-5s, and a total ultrasonic time of 3-5min.

6. The nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects as described in claim 2, characterized in that, In step (4), the average particle size of the nanopeptide liposomes is 80-200 nm.

7. The nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects as described in claim 1, characterized in that, It also includes the following ingredients: 0.05-3 parts ectoine, 0.1-5 parts arbutin, 0.1-3 parts vitamin E, 0.05-2 parts carnosine, and 0.05-3 parts palmitoyl tripeptide-5.

8. The preparation method of the nano-peptide collagen patch with wrinkle-reducing and fine line-reducing effects as described in claim 7, characterized in that, The specific preparation steps include: S1. Add recombinant collagen, ceramide, hyaluronic acid, ectoine, arbutin, vitamin E, carnosine and palmitoyl tripeptide-5 to water, add nanopeptide liposomes, mix evenly to obtain coating solution. S2. Apply the coating liquid to the substrate, dry it, cover it with an isolation layer, cut it into shape, and obtain the nano-peptide collagen film.

9. The preparation method of a nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects as described in claim 8, characterized in that, In step S1, the total solids content in the coating liquid is 10%-15%.

10. The preparation method of a nano-peptide collagen patch with wrinkle-reducing and fine line-fading effects as described in claim 8, characterized in that, In step S2, the substrate is silk fiber or non-woven fabric; the drying is carried out at 30-35°C until the moisture content is 15wt%-20wt%.

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