Preparation method and application of a conditioning skin compound oil

Abstract

The application relates to a preparation method and application of a compound oil for conditioning skin and belongs to the technical field of cosmetics. The preparation method of the compound oil comprises the following steps: taking camellia oil and hazelnut oil of pure natural plant sources as extraction solvents, taking blue lotus as an extraction raw material, extracting blue lotus flower oil extract by using a vacuum low-temperature microwave technology, and then adding oat kernel oil and an antioxidant to obtain the compound oil. The compound oil has the following advantages: the compound oil can effectively improve skin oxidation and aging phenomena, has soothing effect and mood skin care, and delays the skin aging process from the source; the compound oil is of pure natural plant sources, green and environment-friendly, safe and effective, and free of organic solvent residues; the vacuum low-temperature microwave extraction technology is adopted, so that the aromaticity and the activity of effective components are retained; the compound oil is non-irritating, has a moisturizing and light skin feeling, and is suitable for use on various skin.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a method for preparing and applying a compound oil for conditioning the skin. Background Technology

[0002] With the improvement of living standards, people are paying more and more attention to skin care. Young, healthy skin has a complete epidermal barrier and abundant, well-organized collagen and elastin fibers in the dermis. However, with age and external aggressors, oxidative stress and glycation damage the "support structure" of the dermis, leading to signs of aging such as sagging, wrinkles, dullness, yellowing, and loss of elasticity. Therefore, skincare products that can alleviate oxidative stress and glycation are needed to improve skin condition. Most commercially available skincare oils are extracted using traditional solvent methods at high temperatures, which can easily destroy active ingredients and leave organic solvent residues, contradicting the trend towards green and safe products. Furthermore, some skincare oils have a heavy texture and are not easily absorbed. Summary of the Invention

[0003] The purpose of this invention is to overcome the shortcomings of the prior art and provide a method for preparing and applying a compound oil for conditioning the skin.

[0004] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a method for preparing a compound oil for conditioning the skin, the preparation method comprising the following steps: S1. Wash the blue water lily, dry it, and then crush it to obtain coarse blue water lily powder; S2. An extraction solvent is prepared by mixing camellia oil and hazelnut oil; S3. Set up a vacuum environment, add the extraction solvent to the coarse powder of blue water lily, perform microwave extraction, release the vacuum, separate and remove the coarse powder residue of blue water lily to obtain blue water lily oil extract; S4. Add oat kernel oil and antioxidants to blue water lily oil extract, mix well, and obtain compound oil.

[0005] Egyptian blue water lily is an aquatic plant rich in active ingredients such as flavonoids, anthocyanins, phenols, and polysaccharides. Its extracts are widely used in skincare products. Cellular energy production processes and immune responses, as well as factors such as ultraviolet radiation, air pollution, smoking, and unhealthy diets, can exacerbate the production of free radicals. When free radicals attack skin cells, they damage DNA, destroy collagen and elastin fibers, leading to loss of skin elasticity, wrinkles, and dullness. Flavonoids have powerful antioxidant capabilities, effectively scavenging free radicals and improving signs of aging. Furthermore, their anti-inflammatory and soothing properties help calm sensitivity and repair the skin barrier.

[0006] Camellia oil, also known as tea seed oil, is a woody plant oil extracted from the mature seeds of the camellia tree. Camellia oil has a structure similar to human sebum, making it highly compatible with the skin. It is rich in fatty acids, vitamin E, squalene, and tea polyphenols. These components effectively scavenge free radicals, provide antioxidant benefits, and lock in moisture, while being gentle and non-irritating to the skin.

[0007] Hazelnut oil is a natural plant oil extracted from hazelnut kernels. It is pale yellow to colorless, with a light and fluid texture. Its main active ingredients include fatty acids, phytosterols, vitamins, amino acids, and other trace elements. Hazelnut oil has extremely low saturated fatty acid content and a high proportion of oleic acid, resulting in weak cohesion, low viscosity, and good fluidity. Furthermore, the fatty acid composition of hazelnut oil is very similar to that of human sebum, making it easily absorbed and non-greasy.

[0008] Camellia oil and hazelnut oil are pure natural plant oils, characterized by their green and safe properties. They are non-polar or weakly polar substances, while the active ingredients in blue water lilies (such as aromatic molecules and flavonoids) are mostly weakly to moderately polar. Based on the principle of "like dissolves like," a blend of camellia oil and hazelnut oil can effectively dissolve these components from the blue water lily tissue.

[0009] Oat kernel oil is a natural plant oil extracted from oat seeds. Oat alkaloids are a unique component of oat kernel oil, possessing potent anti-inflammatory, anti-allergic, and antipruritic effects, and are the core substances responsible for oat's soothing properties. Due to its high proportion of lecithin and trace amounts of residual polysaccharides, it has a slightly stronger affinity for water-soluble components, resulting in a slightly more moisturizing feel on the skin. It exhibits a weakly polar state overall, allowing it to be mixed with camellia oil and hazelnut oil in any proportion. It can also adjust the skin feel of mixtures of camellia oil, hazelnut oil, and water lily extract, making them moisturizing, lightweight, and easily absorbed.

[0010] One of the core goals of emotional skincare is to combat skin problems caused by psychological stress, and 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) is a key enzyme in this process. 11β-HSD1 converts inactive cortisol into active cortisol. Cortisol is a glucocorticoid secreted by the adrenal glands, whose main function is to help the body cope with various stresses. When a person is under stress or anxious, the body secretes cortisol, which in turn damages the skin barrier, triggers inflammation, and leads to dry, red, and sensitive skin—commonly known as "emotional skin." Therefore, the core strategy of emotional skincare is to inhibit the overexpression or activity of 11β-HSD1, thereby blocking the damage of stress to the skin.

[0011] This invention uses camellia oil and hazelnut oil, both derived from pure natural plants, as extraction solvents, and blue water lily as the extraction raw material. A blue water lily oil extract is obtained using vacuum low-temperature microwave technology. Oat kernel oil and antioxidants are then added, resulting in a compound oil that retains the original aroma of the plants and possesses powerful antioxidant and anti-cortisol effects. This invention conforms to the principles of green chemistry, is naturally safe, gentle, and effective, and is suitable for all skin types, representing a novel skincare product.

[0012] Furthermore, the mass ratio of camellia oil and hazelnut oil in step S2 is 1:1-5.

[0013] Further, the method for preparing camellia oil in step S2 is as follows: wash and dry the tea seeds, remove the outer shell of the tea seeds, keep only the kernels, and crush them; use a hydraulic press to physically press the seeds at a low temperature (<50℃) to obtain crude oil; remove the solid residue from the crude oil to obtain clear, impurity-free cold-pressed virgin oil, which is camellia oil. The method for preparing hazelnut oil is as follows: remove impurities from hazelnuts, wash, dry, crush into coarse particles, and physically press them using a hydraulic press at a low temperature (<50℃) to obtain crude oil; remove solid residue from the crude oil to obtain clear, impurity-free cold-pressed virgin oil, which is hazelnut oil.

[0014] Furthermore, the vacuum environment set in step S3 is set to a vacuum degree of 0.03-0.08 MPa; Furthermore, in step S3, the ratio of blue water lily powder to extraction solvent is 1:10-20mL.

[0015] Furthermore, in step S3, the flow rate of the extraction solvent added to the coarse blue water lily powder is 10-20 mL / min.

[0016] Furthermore, the microwave extraction conditions described in step S3 are: operating power 450-550W, temperature 25-35℃, and time 10-20 min; Furthermore, the mass ratio of the blue water lily oil extract, oat kernel oil, and antioxidant in step S4 is 1:0.5-1:0.1-0.5.

[0017] Further, the preparation method of oat kernel oil in step S4 is as follows: select plump, mold-free oat kernels and remove impurities; heat the oat kernels after removing impurities at 90-110℃ for 20-40 min to inactivate enzymes; dry and pulverize the enzyme-inactivated oat kernels, and physically press them using a hydraulic press at low temperature (<50℃) to obtain crude oil; remove solid residues from the crude oil to obtain clear, impurity-free cold-pressed virgin oil, which is oat kernel oil.

[0018] Furthermore, the antioxidants mentioned in step S5 include, but are not limited to, tocopherol, tocopheryl acetate, rosemary extract, tea polyphenol palmitate, and ascorbyl palmitate.

[0019] Secondly, the present invention provides a compound oil prepared by the preparation method described in the first aspect.

[0020] Thirdly, the present invention provides the application of the compound oil described in the second aspect in the preparation of cosmetics.

[0021] Furthermore, the cosmetic is a cosmetic with anti-aging, soothing and repairing, and / or brightening and yellowing-reducing effects.

[0022] Fourthly, the present invention provides a cosmetic containing the compound oil described in the second aspect and cosmetic-grade excipients.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides a method for preparing a compound oil for conditioning the skin. The method includes using pure natural plant-derived camellia oil and hazelnut oil as extraction solvents, using blue water lily as the extraction raw material, extracting using vacuum low-temperature microwave technology, and then adding oat kernel oil and antioxidants. The compound oil has the following advantages: (1) Effectively improves skin oxidation and aging, while also having a soothing effect, providing emotional skincare and delaying the skin aging process from the source; (2) Pure natural plant source, green and environmentally friendly, no organic solvent residue, safe and effective; (3) Vacuum low-temperature microwave extraction technology is used to preserve the aroma and activity of the active ingredients; (4) Non-irritating, with a moisturizing and lightweight feel, suitable for all skin types. Detailed Implementation

[0024] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0025] The following examples illustrate the preparation methods of the components used: Camellia oil: Wash the tea seeds, dry them, remove the outer shell, keeping only the kernel, and crush them; use a hydraulic press to physically press the oil at 35℃ to obtain crude oil; remove solid residue from the crude oil through a filter to obtain clear, impurity-free cold-pressed virgin oil; put the filtered oil into dark glass bottles, label them, and store them away from light.

[0026] Hazelnut oil: Remove impurities from hazelnuts, wash and dry them; crush the hazelnuts into coarse particles and physically press them at 35°C using a hydraulic press to obtain crude oil; remove solid residue from the crude oil through a filter to obtain clear, impurity-free cold-pressed virgin oil; store the filtered oil in dark glass bottles, label them, and protect them from light.

[0027] Oat kernel oil: Select plump, mold-free oat kernels and remove impurities; heat the oat kernels at 100℃ for 30 minutes to inactivate enzymes, dry and crush them, and use a hydraulic press at 35℃ to physically press them to obtain crude oil; remove solid residues from the crude oil through a filter to obtain clear, impurity-free cold-pressed virgin oil; put the filtered oil into dark glass bottles, label them, and store them away from light.

[0028] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0029] Example 1 This embodiment provides a method for preparing a compound oil for conditioning the skin, the steps of which are as follows: (1) Wash the blue water lily, dry it, and then crush it; (2) Mix camellia oil and hazelnut oil in a mass ratio of 1:2 evenly to obtain the extraction solvent; (3) Place the coarse powder of blue water lily into the extraction tank and the extraction solvent into the external solvent bottle; the ratio of the coarse powder of blue water lily to the extraction solvent is 1g:15mL; (4) Seal the extraction tank, draw a vacuum, set the vacuum degree to 0.06 MPa, and start the internal circulation system of coolant; (5) Add the extraction solvent to the extraction tank at a flow rate of 15 mL / min, start the microwave, set the operating power to 500W, the temperature to 30℃, and the time to 15 min; (6) Release the vacuum and separate the blue water lily residue to obtain blue water lily oil extract; (7) Add oat kernel oil and tocopherol to blue water lily oil extract at a mass ratio of 1:0.8:0.2, mix, and obtain compound oil; Example 2 This embodiment provides a method for preparing a compound oil for conditioning the skin, the steps of which are as follows: (1) Wash the blue water lily, dry it, and then crush it; (2) Mix camellia oil and hazelnut oil in a mass ratio of 1:1 evenly to obtain the extraction solvent; (3) Place the coarse powder of blue water lily into the extraction tank and the extraction solvent into the external solvent bottle; the ratio of the coarse powder of blue water lily to the extraction solvent is 1g:10mL; (4) Seal the extraction tank, draw a vacuum, set the vacuum degree to 0.08 MPa, and start the internal circulation system of coolant; (5) Add the extraction solvent to the extraction tank at a flow rate of 10 mL / min, start the microwave, set the operating power to 450W, the temperature to 25℃, and the time to 20 min; (6) Release the vacuum and separate the blue water lily residue to obtain blue water lily oil extract; (7) Add oat kernel oil and rosemary extract to blue water lily oil extract in a mass ratio of 1:0.5:0.1, mix, and obtain compound oil; Example 3 This embodiment provides a method for preparing a compound oil for conditioning the skin, the steps of which are as follows: (1) Wash the blue water lily, dry it, and then crush it; (2) Mix camellia oil and hazelnut oil in a mass ratio of 1:5 evenly to obtain the extraction solvent; (3) Place the coarse powder of blue water lily into the extraction tank and the extraction solvent into the external solvent bottle; the ratio of the coarse powder of blue water lily to the extraction solvent is 1g:20mL; (4) Seal the extraction tank, draw a vacuum, set the vacuum degree to 0.03 MPa, and start the internal circulation system of coolant; (5) Add the extraction solvent to the extraction tank at a flow rate of 20 mL / min, start the microwave, set the operating power to 550W, the temperature to 35℃, and the time to 10 min; (6) Release the vacuum and separate the blue water lily residue to obtain blue water lily oil extract; (7) Add oat kernel oil and tocopheryl acetate to blue water lily oil extract at a mass ratio of 1:1:0.5, mix, and obtain compound oil; Comparative Example 1 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference from Example 1 is that the ratio of solvent components extracted in step (2) is different. Specifically, the solvent is a mixture of camellia oil and hazelnut oil with a mass ratio of 1:0.5. The remaining steps and parameters are the same as in Example 1, and compound oil 1 is prepared.

[0030] Comparative Example 2 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference from Example 1 is that the ratio of solvent components extracted in step (2) is different. Specifically, the solvent is a mixture of camellia oil and hazelnut oil with a mass ratio of 1:7. The remaining steps and parameters are the same as in Example 1, and compound oil 2 is prepared.

[0031] Comparative Example 3 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference from Example 1 is that the components of the solvent extracted in step (2) are different. Specifically, the solvent is a combination of avocado oil and hazelnut oil in a mass ratio of 1:2. The remaining steps and parameters are the same as in Example 1, and compound oil 3 is prepared.

[0032] Comparative Example 4 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference from Example 1 is that the components of the solvent extracted in step (2) are different. Specifically, the solvent is a mixture of perilla oil and hazelnut oil in a mass ratio of 1:2. The remaining steps and parameters are the same as in Example 1, and compound oil 4 is prepared.

[0033] Comparative Example 5 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference from Example 1 is that step (2) uses only a single plant oil as the extraction solvent, specifically: the extraction solvent is camellia oil. The remaining steps and parameters are the same as in Example 1, and compound oil 5 is prepared.

[0034] Comparative Example 6 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference from Example 1 is that step (2) uses only a single plant oil as the extraction solvent, specifically: the extraction solvent is hazelnut oil; The remaining steps and parameters are the same as in Example 1, and compound oil 6 is prepared.

[0035] Comparative Example 7 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference from Example 1 is that step (3) is different. Specifically, blue water lily powder is put into an extraction tank and the extraction solvent is put into an external solvent bottle. The ratio of blue water lily powder to extraction solvent is 1g:30mL. The remaining steps and parameters are the same as in Example 1, and the compound oil 7 is prepared.

[0036] Comparative Example 8 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference from Example 1 is that step (3) is different. Specifically, blue water lily powder is put into an extraction tank and the extraction solvent is put into an external solvent bottle. The ratio of blue water lily powder to extraction solvent is 1g:5mL. The remaining steps and parameters are the same as in Example 1, and compound oil 8 is prepared.

[0037] Comparative Example 9 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference between this method and Example 1 lies in the extraction technique. The specific preparation method includes the following steps: (1) Wash the blue water lily, dry it, and then crush it; (2) Mix camellia oil and hazelnut oil in a mass ratio of 1:2 evenly to obtain the extraction solvent; (3) Mix the coarse powder of blue water lily with a mixture of camellia oil and hazelnut oil at room temperature and soak for 30 min to obtain a mixture containing coarse powder of blue water lily; the ratio of coarse powder of blue water lily to extraction solvent is 1g:15mL. (4) Place the mixture containing coarse powder of blue water lily into an ultrasonic machine, set the operating power to 200W, and the time to 30min; (5) Filter the blue water lily residue to obtain blue water lily oil extract; (6) Add oat kernel oil and antioxidant to blue water lily oil extract at a mass ratio of 1:0.8:0.2, mix, and obtain compound oil 9.

[0038] Comparative Example 10 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference between this method and Example 1 lies in the extraction technique. The specific preparation method includes the following steps: (1) Wash the blue water lily, dry it, and then crush it; (2) Mix camellia oil and hazelnut oil in a mass ratio of 1:2 evenly to obtain the extraction solvent; (3) Mix the coarse powder of blue water lily with the extraction solvent evenly and soak for 1 h; the ratio of the coarse powder of blue water lily to the extraction solvent is 1 g: 15 mL; (4) Filter the blue water lily residue to obtain blue water lily oil extract; (5) Add oat kernel oil and antioxidant to blue water lily oil extract at a mass ratio of 1:0.8:0.2, mix, and obtain compound oil 10.

[0039] Comparative Example 11 This comparative example provides a method for preparing a compound oil for conditioning the skin. The difference from Example 1 is that vacuum extraction is not used. The specific preparation method includes the following steps: (1) Wash the blue water lily, dry it, and then crush it; (2) Mix camellia oil and hazelnut oil in a mass ratio of 1:2 evenly to obtain the extraction solvent; (3) Mix the coarse powder of blue water lily with the mixed oil of camellia oil and hazelnut oil evenly and soak for 30 min; the ratio of the coarse powder of blue water lily to the extraction solvent is 1g:15mL; (4) Place the mixture into a microwave extraction device, set the operating power to 500W, and the time to 15 min; (5) Filter the blue water lily residue to obtain blue water lily oil extract; (6) Oat kernel oil and antioxidant were added to the purified blue water lily oil extract at a mass ratio of 1:0.8:0.2 and mixed to obtain compound oil 11.

[0040] Test Example 1: Effect of compound oils on the antioxidant capacity of human skin fibroblasts (HSF) This test case examines the effect of compound oil on the antioxidant capacity of HSF cells. The compound oils prepared in Examples 1-3 and Comparative Examples 1-11 were used as test samples to evaluate their effect on oxidative aging of HSF cells.

[0041] Test principle: Cells were stimulated with 200 μM H2O2 for 4 h and then cultured for 24 h to construct a cell senescence model. SA-β-gal staining utilizes the highly active β-galactosidase in senescent cells at pH 6.0 to catalyze the formation of an insoluble blue precipitate from the X-gal substrate, thereby visually marking senescent cells.

[0042] The cell line used was human skin fibroblasts (HSF) (Shanghai Cell Bank, Chinese Academy of Sciences). The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by testing. The testing methods are as follows: (1) Sample preparation: The compound oils prepared in Examples 1-3 and Comparative Examples 1-11 were diluted respectively, and the final concentration of the experiment was 1% (v / v). (2) Seed cell suspension in 24-well plates, add 1 mL of DMEM culture medium (DME101500, Jetech) containing 10% v / v FBS to each well, with a density of 100,000 cells / well, and culture for 24 h; (3) The original culture medium of each group was discarded. The control group and the hydrogen peroxide damage group were respectively added with 1 mL of DMEM medium containing 10% v / v FBS; 1 mL of sample solution was added to each sample group. Each group treatment was set up in 3 parallel experiments. (4) Except for the control group, each group was given 0.5 mL of H2O2 (88597-100mL-F, Sigma) solution with a concentration of 3000 μmol / L. The control group was given 0.5 mL of DMEM medium containing 10% v / v FBS. Each group was cultured for 24 h. (5) β-galactosidase assay: Follow the instructions for the test kit (G1580, Solarbio) and calculate the staining positivity rate (%): Staining positivity rate (%) = (Number of stained cells / Total number of cells) × 100%; The lower the staining positivity rate, the stronger the anti-cellular aging ability of the compound oil.

[0043] The results are averaged, as shown in Table 1. The data from Examples 1-3 show that the compound oil prepared by this invention has a strong anti-cellular aging ability. However, considering the data from Examples 1-3 and Comparative Examples 1-11, the anti-cellular aging ability of Comparative Examples 1-11 is poor. This indicates that in the preparation process of the compound oil, the extraction method (Comparative Examples 9-11), the material-to-liquid ratio (Comparative Examples 7-8), the composition of the extraction solvent (Comparative Examples 3-6), and the ratio of the extraction solvent (Comparative Examples 1-2) all have a significant impact on the anti-cellular aging ability of the prepared compound oil.

[0044] Table 1. Results of HSF cell staining positivity rate Examples / Comparative Examples Concentration (v / v) % positive staining rate control group / 2.362% hydrogen peroxide group / 10.317% Example 1 1% 0.319% Example 2 1% 0.338% Example 3 1% 0.351% Comparative Example 1 1% 0.457% Comparative Example 2 1% 0.463% Comparative Example 3 1% 0.616% Comparative Example 4 1% 0.589% Comparative Example 5 1% 0.365% Comparative Example 6 1% 0.611% Comparative Example 7 1% 0.395% Comparative Example 8 1% 0.391% Comparative Example 9 1% 0.528% Comparative Example 10 1% 0.594% Comparative Example 11 1% 0.473% Test Example 2: Effect of compound oil on the activity of human skin fibroblasts (HSF) This test case investigates the effect of compound oil on HSF cell viability. The compound oils prepared in Examples 1-3 and Comparative Examples 1-11 were used as test samples to evaluate their effect on HSF cell viability.

[0045] Test principle: In the CCK-8 assay, WST-8 in the reagent is reduced by dehydrogenases in living cells to produce orange-yellow formazan dye. The amount of dye is directly proportional to the number of living cells. The absorbance (OD value) is measured at 450 nm using a microplate reader, which indirectly reflects the metabolic activity, proliferation capacity, and viability of the cells. A higher OD value indicates a higher cell number or viability; a lower OD value indicates a lower cell number or viability.

[0046] The cell line used was human skin fibroblasts (HSF) (Shanghai Cell Bank, Chinese Academy of Sciences). The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by testing. The testing methods are as follows: (1) Sample preparation: The compound oils prepared in Examples 1-3 and Comparative Examples 1-11 were diluted respectively, and the final concentration of the experiment was 1% (v / v). (2) In a 96-well plate, seed 100 μL of cell suspension per well with DMEM culture medium containing 10% v / v FBS, at a density of 10,000 cells / well. (3) The original culture medium of each group was discarded. The control group and the hydrogen peroxide damage group (H2O2 group) were respectively added with 100 μL of DMEM medium (DME101500, Jete) containing 10% v / v FBS; 100 μL of sample solution was added to each sample group. Each group treatment was set up in 3 parallel experiments. (4) Except for the control group, each group was given 50 μL of H2O2 (88597-100mL-F, Sigma) solution with a concentration of 3000 μmol / L. The control group was given 50 μL of DMEM medium containing 10% v / v FBS. Each group was cultured for 24 h. (5) Discard the original culture medium and add 100 μL of culture medium containing CCK-8 (B34304, Selleckchem) to each well (DMEM medium: CCK-8 = 10:1). Continue to culture at 37±1℃ for 1 h and measure the absorbance value at 450 nm wavelength.

[0047] Calculate the damage inhibition rate for each sample group: Damage inhibition rate % = [(OD value of sample group - OD value of H2O2 group) / (OD value of control group - OD value of H2O2 group)] × 100%.

[0048] The results were averaged, as shown in Table 2. The compound oils prepared in Examples 1-3 showed the highest inhibition rate of HSF cell damage, reaching 80.11%, indicating that the compound oils prepared in this invention have a strong inhibitory ability against oxidative damage. Comparing the data of Comparative Examples 1-11, the inhibition rate of HSF cell damage in Comparative Examples 1-11 was much lower than that in Examples 1-3, while the inhibition rate of damage in Comparative Examples 3-6 was even lower. This indicates that the effect of the compound oils prepared in this invention is closely related to the extraction solvent. At the same time, the extraction method (Comparative Examples 9-11), the material-to-liquid ratio (Comparative Examples 7-8), and the extraction solvent ratio (Comparative Examples 1-2) all affect the ability of the compound oils to inhibit oxidative damage.

[0049] Table 2 Results of the inhibitory effect of compound oil on HSF cell damage Examples / Comparative Examples Concentration (v / v) Damage inhibition rate % Example 1 1% 80.11% Example 2 1% 79.85% Example 3 1% 79.73% Comparative Example 1 1% 78.63% Comparative Example 2 1% 75.28% Comparative Example 3 1% 68.46% Comparative Example 4 1% 69.81% Comparative Example 5 1% 73.39% Comparative Example 6 1% 65.40% Comparative Example 7 1% 78.37% Comparative Example 8 1% 79.54% Comparative Example 9 1% 70.63% Comparative Example 10 1% 69.98% Comparative Example 11 1% 73.35% Test Example 3: Effect of compound oil on the cortisol resistance of human immortalized epidermal cells (HaCaT) This test case investigates the effect of compound oil on the anticortisol ability of HaCaT. The compound oil prepared in Example 1 is used as the test sample to evaluate the effect on the anticortisol ability of HaCaT at different concentrations.

[0050] Test principle: Cells were cultured for 24 hours with 100 μM cortisone to promote the expression of 11β-HSD1. The gene expression level of 11β-HSD1 was detected by qPCR, which can indirectly reflect the effect of cells on the expression of 11β-HSD1.

[0051] The cell line used was immortalized human epidermal cells (HaCaT) (Shanghai Cell Bank, Chinese Academy of Sciences). The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by testing. The testing methods are as follows: (1) Sample preparation: The compound oil prepared in Example 1 was diluted to achieve final concentrations of 0.1% (v / v), 0.5% (v / v), and 1% (v / v). (2) Seed cell suspension in 24-well plates, add 1 mL of DMEM culture medium (DME101500, Jetech) containing 10% v / v FBS to each well, with a density of 100,000 cells / well, and culture for 24 h; (3) The original culture medium of each group was discarded. The control group and the cortisone treatment group were respectively added with 1 mL of DMEM medium containing 10% v / v FBS; 1 mL of sample solution was added to each sample group. Each group treatment was set up with 3 parallel experiments. (4) Except for the control group, each group was given 0.5 mL of cortisone solution with a concentration of 100 μmol / L. The control group was given 0.5 mL of DMEM medium containing 10% v / v FBS. Each group was cultured for another 24 h. (5) Discard the supernatant, wash with PBS, add 0.5 mL of lysis buffer to each well, extract RNA, reverse transcribe to cDNA, and perform real-time PCR detection. -ΔΔCT The method is used to calculate the results.

[0052] The results are averaged, as shown in Table 3. The data from Example 1 show that the compound oil prepared by the present invention has good anti-cortisol effects.

[0053] Table 3. Relative gene expression levels of 11β-HSD1 in HaCaT cells Example Concentration (v / v) 11β-HSD1 gene relative expression level control group / 1 Cortisone treatment group / 1.33 Example 1 0.1% 0.91 Example 1 0.5% 0.70 Example 1 1% 0.52 Test Example 4: Oxidative Stability Test of Compound Oil This test example refers to the method of QB / T 4079-2010, and the peroxide value and acid value of Examples 1-3 and Comparative Examples 1-11 were determined after one month of storage (room temperature in the dark, 48°C in the dark, and 25°C under natural light). The results are as follows: Table 4 shows that under conditions of 48℃ in the dark and under natural light, the peroxide value of the compound oil increases after one month of storage. In Examples 1-3, after one month of storage under different conditions, the peroxide value was ≤10 mmol / kg, indicating that the product can be stably stored and used. In Comparative Examples 3 and 4, when camellia oil was replaced with avocado oil or perilla oil, the peroxide value was higher, indicating that camellia oil is relatively more stable under this extraction process.

[0054] As shown in Table 5, under different conditions, the acid value of the compound oil will increase after one month of storage, but the change is not significant and is ≤5 mg KOH / g, which meets the requirements of QB / T 4079-2010.

[0055] Table 4 Peroxide value of compound oils Table 5 Acid Value of Blended Oils Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A method for preparing a compound oil for conditioning the skin, characterized in that, The preparation method includes the following steps: S1. Wash the blue water lily, dry it, and then crush it to obtain coarse blue water lily powder; S2. An extraction solvent is prepared by mixing camellia oil and hazelnut oil; S3. Set up a vacuum environment, add the extraction solvent to the coarse powder of blue water lily, perform microwave extraction, release the vacuum, separate and remove the coarse powder residue of blue water lily, and obtain blue water lily oil extract; S4. Add oat kernel oil and antioxidants to blue water lily oil extract, mix well, and obtain compound oil.

2. The preparation method according to claim 1, characterized in that, The mass ratio of camellia oil and hazelnut oil in step S2 is 1:1-5.

3. The preparation method according to claim 1, characterized in that, The vacuum environment setting mentioned in step S3 is to set the vacuum degree to 0.03-0.08 MPa.

4. The preparation method according to claim 1, characterized in that, In step S3, the ratio of coarse blue water lily powder to extraction solvent is 1:10-20mL.

5. The preparation method according to claim 1, characterized in that, The microwave extraction conditions described in step S3 are: operating power 450-550W, temperature 25-35℃, and time 10-20 min.

6. The preparation method according to claim 1, characterized in that, The mass ratio of the blue water lily oil extract, oat kernel oil and antioxidant mentioned in step S4 is 1:0.5-1:0.1-0.

5.

7. The compound oil prepared by the method according to any one of claims 1-6.

8. The application of the compound oil according to claim 7 in the preparation of cosmetics.

9. The application as described in claim 8, characterized in that, The cosmetics mentioned are cosmetics with anti-aging, soothing and repairing and / or brightening effects.

10. A cosmetic product, characterized in that, It contains the compound oil as described in claim 7 and excipients acceptable in the cosmetics field.

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