A composition with whitening teeth efficacy and its preparation method and application

Abstract

The application discloses a composition with tooth whitening efficacy and a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition comprises the following efficacy components in parts by weight: saussurea involucrata extract 1.0-5.0 parts, clove bud extract 0.1-1.0 parts, cordyceps militaris extract 0.1-0.5 parts, poria cocos extract 1.0-5.0 parts, Lepidium sativum seed extract 0.1-1.0 parts, European fir leaf oil 0.05-0.2 parts and pineapple fruit extract 0.1-1.0 parts. Seven kinds of efficacy raw materials of natural origin are scientifically compounded, and the tooth whitening and bacteriostasis effects are realized in a multi-pathway synergistic mode. The composition is safe and non-irritating, the preparation process is simple, industrialized production is suitable, and the composition can be used for preparing various tooth cosmetics.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a composition with teeth whitening effect, its preparation method and application. Background Technology

[0002] Tooth discoloration is mainly divided into extrinsic discoloration and intrinsic discoloration: extrinsic discoloration is caused by pigments in food (such as tea, coffee, and red wine), tobacco, and pigments produced by oral bacteria deposited on the tooth surface; intrinsic discoloration is caused by factors such as drugs ingested during tooth development, excessive fluoride, and pulp necrosis.

[0003] Currently, teeth whitening products on the market are mainly divided into two categories: chemical whitening and natural whitening. Chemical whitening products often use strong oxidizing ingredients such as hydrogen peroxide and urea peroxide. Although they can achieve a whitening effect quickly, long-term use can irritate the oral mucosa, damage tooth enamel, and lead to problems such as tooth sensitivity and bleeding gums, making them less safe. Natural whitening products mostly use single plant extracts, which have drawbacks such as a gentle whitening effect but slow results, a single whitening mechanism, and an inability to take into account oral care, making it difficult to meet consumers' dual needs for whitening effect and oral safety and care.

[0004] To address the aforementioned issues, existing technologies have made some attempts at improvement. CN202511701743.6 discloses a toothpaste with whitening effects and its preparation method, including plant extracts, humectants, thickeners, abrasives, foaming agents, and oral care agents. The plant extracts include grape seed extract, pomegranate extract, angelica extract, and peony bark extract. This toothpaste has a certain whitening effect, but the whitening mechanism of the plant extracts is singular, and the antibacterial effect is not explicitly mentioned, making it unable to effectively regulate the oral microecology. CN202511536724.2 discloses a teeth-whitening toothpaste and its preparation method. This toothpaste includes, by mass percentage, 20-30% tomato concentrate, 5-10% eggshell powder, and 0.5-2% cinnamon oil, etc., achieving a whitening effect through a triple mechanism of natural hydrogen peroxide whitening, gentle grinding with calcium carbonate, and natural antibacterial action. However, the patent contains a high concentration of tomato extract, which is highly acidic. Long-term use may corrode tooth enamel. Furthermore, the whitening effect mainly relies on physical abrasion and natural hydrogen peroxide, which has limited depth of action and is difficult to remove pigments from the dentin layer.

[0005] Therefore, developing a composition with significant whitening effect, strong antibacterial ability, and safety without irritation that has teeth whitening effect remains a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0006] The technical problem this invention aims to solve is to overcome the shortcomings of existing teeth whitening products, such as high irritation, a single whitening mechanism, and poor antibacterial effect. This invention provides a composition with teeth whitening efficacy, its preparation method, and its application. All active ingredients in this invention are plant-derived extracts, avoiding the irritation and ecotoxicity problems of chemical whitening products such as hydrogen peroxide and urea peroxide. Through the scientific compounding of seven naturally derived active ingredients, multiple pathways are synergistically used to achieve teeth whitening and antibacterial effects, while remaining safe and non-irritating.

[0007] To solve the above-mentioned technical problems, the present invention provides the following technical solution: In a first aspect, the present invention provides a composition having teeth whitening effects, comprising, by weight, the following functional components: Snow lotus extract 1.0-5.0 parts; Clove bud extract, 0.1-1.0 parts; Cordyceps militaris extract, 0.1-0.5 parts; Poria cocos extract 1.0-5.0 parts; 0.1-1.0 part of rapeseed extract; 0.05-0.2 parts of European fir leaf oil; Pineapple fruit extract 0.1-1.0 parts.

[0008] Preferably, the active ingredients are included in parts by weight: Snow lotus extract 2.0-4.0 parts; Clove bud extract, 0.3-0.7 parts; Cordyceps militaris extract, 0.2-0.4 parts; Poria cocos extract, 2.0-4.0 parts; 0.2-0.7 parts of rapeseed extract; 0.1-0.15 parts of European fir leaf oil; Pineapple fruit extract 0.3-0.8 parts.

[0009] Snow lotus extract is rich in active ingredients such as flavonoids, polysaccharides, and alkaloids. It has a strong antioxidant capacity, which can effectively remove free radicals in the mouth, reduce the damage of oxidative stress to teeth, and prevent teeth from yellowing due to oxidation. At the same time, it can inhibit the growth of melanin-producing bacteria, reduce the production of endogenous pigments, and prevent tooth discoloration from the source.

[0010] Preferably, the snow lotus extract can be in the following weight parts: 1.0 part, 2.0 part, 3.0 part, 4.0 part, or 5.0 part; more preferably, it is 2.0-4.0 parts.

[0011] Clove bud extract contains volatile components such as eugenol and acetyleugenol, which have a significant inhibitory effect on major oral pathogens such as Porphyromonas gingivalis, effectively reducing plaque formation and preventing tooth decay and periodontal disease; it also has a certain anti-inflammatory effect, which can relieve symptoms such as gingival redness and bleeding.

[0012] Preferably, the clove bud extract can be in the following weight parts: 0.1 parts, 0.3 parts, 0.5 parts, 0.7 parts, or 1.0 parts; more preferably, it is 0.3-0.7 parts.

[0013] Cordyceps militaris extract contains a variety of bioactive substances such as cordycepin, cordyceps polysaccharides, and adenosine. It has a broad-spectrum antibacterial effect, can inhibit the growth and reproduction of harmful bacteria in the oral cavity, and regulate the balance of oral microecology. At the same time, it can enhance the body's immunity, improve the resistance of oral tissues, and promote the repair of damaged gingival tissues.

[0014] Preferably, the Cordyceps militaris extract can be in the following weight proportions: 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, or 0.5 parts; more preferably, it is 0.2-0.4 parts.

[0015] Poria cocos extract competitively inhibits the adsorption and deposition of pigment molecules by binding to tooth surface proteins. At the same time, its polysaccharide components can form a protective film on the tooth surface, reducing subsequent pigment adhesion.

[0016] Preferably, the weight parts of the Poria cocos extract can be 1.0 parts, 2.0 parts, 3.0 parts, 4.0 parts, or 5.0 parts; more preferably, it is 2.0-4.0 parts.

[0017] Rapeseed extract contains abundant flavonoids and phenolic acids, which have excellent antioxidant activity. It can effectively remove free radicals in the mouth and reduce the oxidative darkening of teeth. At the same time, it can soften the protein biofilm on the tooth surface, help remove pigment stains attached to the tooth surface, and improve the whitening effect.

[0018] Preferably, the weight parts of the rapeseed extract can be 0.1 parts, 0.2 parts, 0.5 parts, 0.7 parts, or 1.0 parts; more preferably, it is 0.2-0.7 parts.

[0019] European fir leaf oil has good fat solubility, which can quickly penetrate the tooth surface biofilm, promote the absorption of other active ingredients, and improve whitening efficiency; at the same time, it has a natural fresh aroma, which can effectively cover up bad breath and keep breath fresh; in addition, it also has a certain antibacterial effect, which can help inhibit harmful bacteria in the mouth.

[0020] Preferably, the weight parts of European fir leaf oil can be 0.05 parts, 0.1 parts, 0.12 parts, 0.15 parts, or 0.2 parts; more preferably, it is 0.1-0.15 parts.

[0021] Pineapple fruit extract is rich in bromelain, a proteolytic enzyme that can effectively degrade the protein components in dental plaque biofilm, destroy the plaque structure, and make pigment deposits easier to remove; at the same time, it can break down food residue and reduce bad breath.

[0022] Preferably, the pineapple fruit extract can be in the following weight parts: 0.1 parts, 0.3 parts, 0.5 parts, 0.8 parts, or 1.0 parts; more preferably, it is 0.3-0.8 parts.

[0023] In a preferred embodiment of the present invention, the seven active ingredients exhibit significant synergistic effects: First, European fir leaf oil rapidly penetrates the tooth surface biofilm, opening channels for other active ingredients; second, bromelain degrades the protein structure of the dental plaque biofilm, and rapeseed extract softens the remaining biofilm components, the two working synergistically to thoroughly destroy the plaque biofilm, exposing pigment deposits; then, Poria cocos extract competitively inhibits the adsorption and deposition of pigment molecules by binding to tooth surface proteins, while snow lotus extract and rapeseed extract scavenge free radicals through antioxidant effects, reducing tooth oxidative darkening. Simultaneously, snow lotus extract also inhibits melanin-producing bacteria, reducing endogenous pigment production; finally, clove bud extract and Cordyceps militaris extract synergistically inhibit harmful oral bacteria, regulate the oral microecological balance, prevent plaque reformation, and thus maintain the whitening effect. Furthermore, all ingredients are of natural origin, safe and non-irritating, and will not damage tooth enamel or gums.

[0024] More preferably, the composition further includes the following excipient components: Moisturizer 5.0-20.0 parts; Friction agent 10.0-30.0 parts; Surfactant 0.5-3.0 parts; Thickener 0.1-1.0 parts; Preservative: 0.05-0.2 parts; Fragrance 0.05-0.3 parts; Prepare 100 parts of deionized water.

[0025] More preferably, the humectant is selected from one or more of glycerin, xylitol, and sorbitol, which can retain the moisture of the ointment, prevent the ointment from drying and hardening, and at the same time have a certain moisturizing effect, which can relieve dry mouth.

[0026] More preferably, the abrasive is selected from one or more of hydrated silica, calcium carbonate, and calcium hydrogen phosphate dihydrate, which can remove plaque and some pigments from the tooth surface through gentle physical friction, thus aiding in the whitening effect.

[0027] More preferably, the surfactant is selected from one or more of sodium lauroyl sarcosinate and cocamidopropyl betaine, which has good foaming and cleaning abilities, can effectively clean the oral cavity, and is gentle and non-irritating.

[0028] More preferably, the thickener is selected from one or more of xanthan gum, sodium carboxymethyl cellulose, and carbomer, which can increase the viscosity of the paste and improve its stability and spreadability.

[0029] More preferably, the preservative is selected from one or more of p-hydroxyacetophenone and 1,2-hexanediol, both of which are mild preservatives with low irritation to the oral mucosa, effectively inhibiting microbial growth and extending the product's shelf life.

[0030] More preferably, the fragrance can be a food-grade fragrance commonly used in the field of oral cosmetics, including but not limited to peppermint fragrance, spearmint fragrance, lemon fragrance, strawberry fragrance, green tea fragrance, etc., and the appropriate fragrance can be selected according to the product positioning and target audience.

[0031] Secondly, the present invention provides a method for preparing the above-mentioned composition with teeth whitening effect, comprising the following steps: S1. Deionized water, humectant, and thickener are added to the reaction vessel and stirred at room temperature until completely dissolved and homogeneous to obtain an aqueous matrix. S2 Add abrasive and surfactant to the aqueous matrix and stir until evenly dispersed to obtain a basic paste; S3. Add snow lotus extract, clove bud extract, cordyceps extract, poria cocos extract, rapeseed extract, fir leaf oil, and pineapple fruit extract to the base paste in sequence, and stir until well mixed. S4 Add preservatives and flavorings, and continue stirring until the system is homogeneous and smooth; S5 Vacuum degassing, filling, to obtain the composition with teeth whitening effect.

[0032] Preferably, in step S1, the stirring speed is 200-400 r / min and the stirring time is 15-30 min; In step S2, the stirring speed is 300-500 r / min and the stirring time is 20-40 min; In step S3, the stirring speed is 200-300 r / min, and the stirring time is 15-25 min; In step S4, the stirring speed is 150-250 r / min and the stirring time is 10-20 min; In step S5, the vacuum degree is -0.06 to -0.08 MPa, and the degassing time is 10-15 min.

[0033] Thirdly, the present invention provides the application of the above-mentioned composition with teeth whitening effect in the preparation of dental cosmetics, wherein the dental cosmetics include, but are not limited to, toothpaste, tooth powder, teeth whitening gel, etc.

[0034] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention utilizes a scientifically formulated blend of seven naturally derived active ingredients: snow lotus extract, clove bud extract, cordyceps extract, poria cocos extract, rapeseed extract, European fir leaf oil, and pineapple fruit extract. This multi-pathway synergistic effect achieves teeth whitening and antibacterial properties. The composition of this invention exhibits significant whitening effects and strong antibacterial capabilities. The European fir leaf oil and other ingredients used in this invention are all permitted ingredients in the cosmetics catalog and have been verified through skin irritation tests to be non-irritating to the skin. Detailed Implementation

[0035] The present invention will be described in detail below with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Unless otherwise specified, the raw materials and reagents used in the embodiments and comparative examples of the present invention can be obtained commercially, and the fragrance selected is peppermint flavoring.

[0036] Examples 1-9 Examples 1-5: Compositions with teeth whitening effects are shown in Table 1. All components are expressed in parts by weight.

[0037] Table 1 Snow lotus extract 1.0 3.0 5.0 3.0 3.0 Clove bud extract 0.1 0.5 1.0 0.5 0.5 Cordyceps militaris extract 0.1 0.3 0.5 0.3 0.3 Poria cocos extract 1.0 3.0 5.0 3.0 3.0 rapeseed extract 0.1 0.5 1.0 0.5 0.5 European fir leaf oil 0.05 0.12 0.2 0.12 0.12 Pineapple fruit extract 0.1 0.5 1.0 0.5 0.5 glycerin 5.0 15.0 15.0 - 5.0 Xylitol - - 5.0 - 5.0 Sorbitol - - - 15.0 5.0 hydrated silica 10.0 20.0 30.0 - 8.0 Calcium carbonate - 5.0 - - 8.0 Calcium hydrogen phosphate dihydrate - - - 25.0 9.0 Sodium lauroyl sarcosinate 0.5 1.5 3.0 1.5 1.0 Cocamidopropyl Betaine - 0.5 - 0.5 1.0 Xanthan Gum 0.1 0.5 1.0 - 0.2 Sodium carboxymethyl cellulose - 0.2 - - 0.2 Carbomer - - - 0.7 0.3 p-Hydroxyacetophenone 0.05 0.1 0.2 - 0.05 1,2-Hexanediol - - - 0.1 0.05 essence 0.05 0.15 0.3 0.15 0.15 Deionized water Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Example 1: A method for preparing a composition with teeth whitening effects includes the following steps: S1. Deionized water, glycerol, and xanthan gum are added to the reaction vessel and stirred at 200 r / min for 30 min at room temperature until completely dissolved and homogeneous to obtain an aqueous matrix. S2 Add hydrated silica and sodium lauroyl sarcosinate to the aqueous matrix, stir at 300 r / min for 40 min until uniformly dispersed to obtain the basic paste; S3. Add snow lotus extract, clove bud extract, cordyceps extract, poria cocos extract, rapeseed extract, European fir leaf oil, and pineapple fruit extract to the base paste in sequence, and stir at 200 r / min for 25 min until well mixed. Add p-hydroxyacetophenone and fragrance to S4, stir at 150 r / min for 20 min, and continue stirring until the system is homogeneous and smooth; S5 is degassed for 15 minutes under a vacuum of -0.06MPa, then filled to obtain the composition with teeth whitening effect.

[0038] Example 2: The preparation method of the composition with teeth whitening effect includes the following steps: S1 Deionized water, glycerol, xanthan gum, and sodium carboxymethyl cellulose were added to a reaction vessel and stirred at 300 r / min for 22 min at room temperature until completely dissolved and homogeneous to obtain an aqueous matrix. S2 Add hydrated silica, calcium carbonate, sodium lauroyl sarcosinate, and cocamidopropyl betaine to the aqueous matrix, and stir at 400 r / min for 30 min until the mixture is evenly dispersed to obtain the basic paste. S3. Add snow lotus extract, clove bud extract, cordyceps extract, poria cocos extract, rapeseed extract, European fir leaf oil, and pineapple fruit extract to the base paste in sequence, and stir at 250 r / min for 20 min until well mixed. Add p-hydroxyacetophenone and fragrance to S4, stir at 200 r / min for 15 min, and continue stirring until the system is homogeneous and smooth; S5 is degassed for 12 minutes under a vacuum of -0.07MPa, then filled to obtain the composition with teeth whitening effect.

[0039] Example 3: The preparation method of the composition with teeth whitening effect includes the following steps: S1. Deionized water, glycerol, xylitol, and xanthan gum are added to the reaction vessel and stirred at 400 r / min for 15 min at room temperature until completely dissolved and homogeneous to obtain an aqueous matrix. S2 Add hydrated silica and sodium lauroyl sarcosinate to the aqueous matrix, stir at 500 r / min for 20 min until uniformly dispersed to obtain the basic paste; S3. Add snow lotus extract, clove bud extract, cordyceps extract, poria cocos extract, rapeseed extract, European fir leaf oil, and pineapple fruit extract to the base paste in sequence, and stir at 300 r / min for 15 min until well mixed. Add p-hydroxyacetophenone and fragrance to S4, stir at 250 r / min for 10 min, and continue stirring until the system is homogeneous and smooth; S5 is degassed for 10 minutes under a vacuum of -0.08MPa, then filled to obtain the composition with teeth whitening effect.

[0040] Example 4: The preparation method of the composition with teeth whitening effect includes the following steps: S1. Deionized water, sorbitol, and carbomer were added to the reactor and stirred at 350 r / min for 20 min at room temperature until completely dissolved and homogeneous to obtain an aqueous matrix. S2 Add calcium hydrogen phosphate dihydrate, sodium lauroyl sarcosinate, and cocamidopropyl betaine to the aqueous matrix, and stir at 450 r / min for 25 min until the mixture is evenly dispersed to obtain the basic paste; S3. Add snow lotus extract, clove bud extract, cordyceps extract, poria cocos extract, rapeseed extract, European fir leaf oil, and pineapple fruit extract to the base paste in sequence, and stir at 280 r / min for 18 min until well mixed. Add 1,2-hexanediol and flavoring to S4, stir at 220 r / min for 12 min, and continue stirring until the system is homogeneous and smooth; S5 is degassed for 13 minutes under a vacuum of -0.07MPa, then filled to obtain the composition with teeth whitening effect.

[0041] Example 5: A method for preparing a composition with teeth whitening effects includes the following steps: S1. Deionized water, glycerol, xylitol, sorbitol, xanthan gum, sodium carboxymethyl cellulose, and carbomer were added to a reaction vessel and stirred at 320 r / min for 25 min at room temperature until completely dissolved and homogeneous to obtain an aqueous matrix. S2 Add hydrated silica, calcium carbonate, calcium hydrogen phosphate dihydrate, sodium lauroyl sarcosinate, and cocamidopropyl betaine to the aqueous matrix, and stir at 420 r / min for 28 min until the mixture is evenly dispersed to obtain the basic paste. S3. Add snow lotus extract, clove bud extract, cordyceps extract, poria cocos extract, rapeseed extract, European fir leaf oil, and pineapple fruit extract to the base paste in sequence, and stir at 260 r / min for 22 min until well mixed. Add p-hydroxyacetophenone, 1,2-hexanediol, and fragrance to S4, and stir at 200 r / min for 14 min. Continue stirring until the system is homogeneous and smooth. S5 is degassed for 12 minutes under a vacuum of -0.07MPa, then filled to obtain the composition with teeth whitening effect.

[0042] Comparative Examples 1-10 The weight parts of the raw materials used in Comparative Examples 1-10 are shown in Table 2. The preparation method is the same as that in Example 2 above (the active ingredients are adjusted according to Table 2, and the rest is the same as in Example 2).

[0043] Table 2 Snow lotus extract - 3.0 3.0 3.0 3.0 3.0 3.0 - 3.0 3.0 Clove bud extract 0.5 - 0.5 0.5 0.5 0.5 0.5 0.5 - 0.5 Cordyceps militaris extract 0.3 0.3 - 0.3 0.3 0.3 0.3 0.3 0.3 - Poria cocos extract 3.0 3.0 3.0 - 3.0 3.0 3.0 3.0 - 3.0 rapeseed extract 0.5 0.5 0.5 0.5 - 0.5 0.5 - 0.5 0.5 European fir leaf oil 0.12 0.12 0.12 0.12 0.12 - 0.12 0.12 0.12 - Pineapple fruit extract 0.5 0.5 0.5 0.5 0.5 0.5 - 0.5 0.5 - glycerin 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 hydrated silica 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 20.0 Calcium carbonate 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Sodium lauroyl sarcosinate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Cocamidopropyl Betaine 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Xanthan Gum 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Sodium carboxymethyl cellulose 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 p-Hydroxyacetophenone 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 essence 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Deionized water Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Effect test 1. Skin irritation test Skin irritation / corrosion tests were conducted according to the "Cosmetic Safety Technical Specifications (2015 Edition)". Adult, healthy, undamaged white rabbits (half male, half female) were randomly divided into 15 groups of 4 rabbits each. Before the test, the animals were acclimatized to the experimental animal facility for 6 days. 24 hours before the test, the hair on both sides of the spine on the back of the experimental animals was shaved, taking care not to damage the epidermis. The shaved area was approximately 3cm × 3cm on each side. 0.5 g of the compositions prepared in Examples 1-5 and Comparative Examples 1-10 was applied directly to the rabbit skin as the test substance. It was then covered with two layers of gauze and one layer of cellophane or similar material, and secured with non-irritating adhesive tape and bandage. The other side of the skin was left untreated as a blank control. A closed-celluloid test was used, with an application time of 6 hours. After the test, the residual test substance was removed with warm water or a non-irritating solvent. Skin reactions at the application site were observed at 1 hour, 24 hours, 48 ​​hours, and 72 hours after removal of the test substance, observing for irritation reactions such as erythema, edema, and erosion.

[0044] 2. Teeth whitening effect test Extracted teeth were stained by soaking in a 0.2% black tea solution at 37°C for 72 hours and then fixed in a custom-made tooth-brushing simulation instrument. 0.5 g of the compositions prepared in Examples 1-5 and Comparative Examples 1-10 were applied to the occlusal surfaces of teeth with stained areas. A simulated brushing session was conducted using an electric toothbrush for 3 minutes at a speed of 300 strokes / minute, a pressure of 150g, and a horizontal brushing mode. During the simulation, the teeth were periodically removed and rinsed thoroughly with deionized water. To characterize the whitening effect of the compositions on teeth, the CIELab system was used to characterize and quantitatively analyze the color changes. This system uses three variables to characterize the color change: ΔL represents the difference in brightness between before and after brushing, Δa represents the color difference along the red-green axis between before and after brushing, and Δb represents the color difference along the blue-yellow axis between before and after brushing. The formula for calculating the color difference (ΔE) of the treated teeth is as follows: .

[0045] 3. Antibacterial effect test The antibacterial effect of the compositions prepared in Examples 1-5 and Comparative Examples 1-10 against *Porphyromonas gingivalis* was determined using the agar diffusion method. *Porphyromonas gingivalis* bacterial suspensions were added at 1% (v / v) to sterilized BHI blood agar plates cooled to approximately 55°C. After thorough mixing, the mixture was poured into petri dishes (15 mL / dish), and the sterile Oxford cups were removed after cooling. The prepared compositions (mixed with deionized water at a 2:1 ratio) of Examples 1-5 and Comparative Examples 1-10 were added to the wells of the cups (200 μL / well) and anaerobically incubated at 37°C. After 24 hours, the diameter of the inhibition zone was measured, with each case repeated three times, and the average value was taken. A larger inhibition zone diameter indicates a better antibacterial effect.

[0046] Test Results 1. Skin irritation test results Skin irritation test results for all compositions prepared in Examples 1-5 and Comparative Examples 1-10 showed that no irritation reactions such as erythema, edema, or erosion occurred on the skin of white rabbits at 1 h, 24 h, 48 h, and 72 h after removal of the test substance. This indicates that the compositions of the present invention and the comparative compositions are safe and non-irritating.

[0047] 2. Results of teeth whitening and antibacterial effect tests The results of the teeth whitening and antibacterial effects tests of the compositions prepared in Examples 1-5 and Comparative Examples 1-10 are shown in Table 3.

[0048] Table 3 Example 1 22.8 16.9 Example 2 25.2 19.8 Example 3 23.7 20.3 Example 4 24.9 19.5 Example 5 25.0 19.6 Comparative Example 1 18.2 16.4 Comparative Example 2 19.1 11.0 Comparative Example 3 19.7 12.5 Comparative Example 4 18.6 16.2 Comparative Example 5 17.9 16.0 Comparative Example 6 17.1 15.8 Comparative Example 7 16.8 15.6 Comparative Example 8 13.3 14.3 Comparative Example 9 14.5 9.7 Comparative Example 10 10.2 10.1 Examples 1-5 showed excellent whitening and antibacterial effects, with ΔE values ​​ranging from 22.8 to 25.2 and inhibition zone diameters of *Porphyromonas gingivalis* ranging from 16.9 to 19.8 mm.

[0049] Compared to Example 2, Comparative Example 1 lacked snow lotus extract, resulting in a decrease in the ΔE value from 25.2 to 18.2, and a reduction in the diameter of the inhibition zone of *Porphyromonas gingivalis*. This indicates that snow lotus extract primarily reduces endogenous pigment production by inhibiting melanin-producing bacteria, while simultaneously scavenging free radicals to alleviate oxidative darkening, making it a significant contributor to the whitening effect of this invention. It also possesses a certain degree of auxiliary antibacterial activity.

[0050] Compared to Example 2, Comparative Example 2 lacked clove bud extract, resulting in a decrease in the ΔE value to 19.1 and a significant reduction in the diameter of the inhibition zone of *Porphyromonas gingivalis* to 11.0 mm. This indicates that clove bud extract is the core antibacterial component of this invention, effectively inhibiting major oral pathogens and reducing plaque formation, thereby indirectly aiding in the whitening effect.

[0051] Compared to Example 2, Comparative Example 3 lacked Cordyceps militaris extract, resulting in a decreased ΔE value of 19.7 and a significantly reduced inhibition zone diameter of 12.5 mm. This indicates that Cordyceps militaris extract and clove bud extract work synergistically to exert antibacterial effects, while also enhancing oral immunity and promoting gingival repair, which is of great significance for maintaining oral health and achieving long-lasting whitening effects.

[0052] Compared to Example 2, Comparative Example 4 lacked Poria cocos extract, resulting in a decrease in the ΔE value to 18.6 and a reduction in the diameter of the inhibition zone. This indicates that Poria cocos extract, by binding to tooth surface proteins and competitively inhibiting the adsorption and deposition of pigment molecules, is a key pathway for removing exogenous pigments. Simultaneously, its polysaccharide components also possess certain auxiliary antibacterial and immunomodulatory effects.

[0053] Compared to Example 2, Comparative Example 5 lacked Rapeseed extract, resulting in a decrease in the ΔE value to 17.9 and a reduction in the diameter of the inhibition zone. This indicates that Rapeseed extract, through its dual effects of softening the tooth surface protein biofilm and antioxidation, can both assist in the removal of pigment stains and reduce tooth oxidative darkening, making it an indispensable part of the whitening process.

[0054] Compared to Example 2, Comparative Example 6 lacked European fir leaf oil, resulting in a decrease in the ΔE value to 17.1 and a reduction in the diameter of the inhibition zone. This indicates that the penetration-enhancing effect of European fir leaf oil is a prerequisite for the effectiveness of all other active ingredients; its absence makes it difficult for active ingredients to penetrate the tooth surface biofilm, and its auxiliary antibacterial effect is also affected.

[0055] Compared to Example 2, Comparative Example 7 lacked pineapple fruit extract, resulting in a decrease in the ΔE value to 16.8. This was the most significant decrease in whitening effect among single-component deficiencies, and the diameter of the inhibition zone also decreased. This indicates that the degradation of dental plaque biofilm by bromelain is fundamental to pigment removal; only by disrupting the plaque structure can other whitening ingredients effectively target pigment deposition.

[0056] Compared to Example 2, Comparative Example 8 lacked both snow lotus extract and *Gynostemma pentaphyllum* seed extract, resulting in a further decrease in the ΔE value to 13.3 and a significant reduction in antibacterial effect. This indicates that these two components have a significant synergistic effect in antioxidation and reducing endogenous pigmentation, while their absence leads to a reduction in the antioxidant effect of the whitening pathway.

[0057] Compared to Example 2, Comparative Example 9 lacked both Poria cocos extract and clove bud extract, resulting in a decrease in the ΔE value to 14.5 and a significant reduction in the diameter of the inhibition zone to 9.7 mm. This indicates that the physical adsorption effect of Poria cocos and the antibacterial effect of clove buds together constitute the basis for the whitening and antibacterial properties of this invention, and their absence leads to a significant decrease in the whitening and antibacterial effects of the product.

[0058] Compared to Example 2, Comparative Example 10 lacked both European fir leaf oil, pineapple fruit extract, and Cordyceps militaris extract, resulting in a ΔE value of only 10.2. This significantly reduced the whitening effect, and the diameter of the antibacterial zone also decreased dramatically to 10.1 mm. This indicates that these three components are the core of the multi-pathway synergistic system of this invention. Their simultaneous absence leads to the failure of all three key aspects: biofilm disruption, active ingredient penetration, and oral microecological regulation, resulting in a substantial decrease in the product's whitening and antibacterial functions.

[0059] In summary, this invention constructs a multi-pathway synergistic teeth whitening and antibacterial system by scientifically compounding seven naturally sourced active ingredients. Each active ingredient works synergistically and is indispensable. All active ingredients in the composition of this invention are plant-derived extracts, making them safe and non-irritating. They possess significant whitening effects and excellent antibacterial capabilities, effectively regulating the oral microecology and maintaining long-lasting whitening effects. This completely solves the technical problems of existing chemical whitening products being highly irritating and natural whitening products being ineffective.

[0060] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition having teeth whitening effect, characterized in that, Based on parts by weight, it includes the following active ingredients: Snow lotus extract 1.0-5.0 parts; Clove bud extract, 0.1-1.0 parts; Cordyceps militaris extract, 0.1-0.5 parts; Poria cocos extract 1.0-5.0 parts; 0.1-1.0 part of rapeseed extract; 0.05-0.2 parts of European fir leaf oil; Pineapple fruit extract 0.1-1.0 parts.

2. The composition with teeth whitening effect according to claim 1, characterized in that, Based on parts by weight, it includes the following active ingredients: Snow lotus extract 2.0-4.0 parts; Clove bud extract, 0.3-0.7 parts; Cordyceps militaris extract, 0.2-0.4 parts; Poria cocos extract, 2.0-4.0 parts; 0.2-0.7 parts of rapeseed extract; 0.1-0.15 parts of European fir leaf oil; Pineapple fruit extract 0.3-0.8 parts.

3. The composition with teeth whitening effect according to claim 1 or 2, characterized in that, It also includes the following excipient components: Moisturizer 5.0-20.0 parts; Friction agent 10.0-30.0 parts; Surfactant 0.5-3.0 parts; Thickener 0.1-1.0 parts; Preservative: 0.05-0.2 parts; Fragrance 0.05-0.3 parts; Prepare 100 parts of deionized water.

4. The composition with teeth whitening effect according to claim 3, characterized in that, The moisturizer is selected from one or more of glycerin, xylitol, and sorbitol.

5. The composition with teeth whitening effect according to claim 3, characterized in that, The friction agent is selected from one or more of hydrated silica, calcium carbonate, and calcium hydrogen phosphate dihydrate.

6. The composition with teeth whitening effect according to claim 3, characterized in that, The surfactant is selected from one or more of sodium lauroyl sarcosinate and cocamidopropyl betaine.

7. The composition with teeth whitening effect according to claim 3, characterized in that, The thickener is selected from one or more of xanthan gum, sodium carboxymethyl cellulose, and carbomer.

8. The composition with teeth whitening effect according to claim 3, characterized in that, The preservative is selected from one or more of p-hydroxyacetophenone and 1,2-hexanediol.

9. A method for preparing a composition having teeth whitening effect as described in any one of claims 3-8, characterized in that, Includes the following steps: S1. Deionized water, humectant, and thickener are added to the reaction vessel and stirred at room temperature until completely dissolved and homogeneous to obtain an aqueous matrix. S2 Add abrasive and surfactant to the aqueous matrix and stir until evenly dispersed to obtain a basic paste; S3. Add snow lotus extract, clove bud extract, cordyceps extract, poria cocos extract, rapeseed extract, fir leaf oil, and pineapple fruit extract to the base paste in sequence, and stir until well mixed. S4 Add preservatives and flavorings, and continue stirring until the system is homogeneous and smooth; S5 Vacuum degassing, filling, to obtain the composition with teeth whitening effect.

10. The use of a composition having teeth-whitening effects as described in any one of claims 1-8 in the preparation of dental cosmetics, characterized in that, The dental cosmetics include toothpaste, tooth powder, and teeth whitening gel.