Anti-aging, nourishing, antioxidant composition containing extracts of olea europaea and camellia seed and method for its preparation

Abstract

The present application relates to an anti-aging, nourishing and antioxidant composition containing oil olive and camellia seed extract and a preparation method thereof, and belongs to the technical field of cosmetics. The composition is composed of hydrolyzed oil olive leaf extract, camellia seed extract, white flower patrinia extract and horsetail extract in a specific mass ratio. The ratio realizes comprehensive anti-aging and skin repair through the synergistic effect of the four plant extracts in anti-aging, nourishing and antioxidant aspects. Experiments show that the composition is significantly better than the control composition lacking of single component or out of proportion in promoting collagen production and inhibiting inflammatory factors. The present application also provides a serum containing the composition, which has the effects of significantly improving wrinkles, improving water content, repairing skin barrier and lightening skin color in human efficacy test, and has high safety. The composition of the present application can be used for preparing various cosmetics such as serum and cream.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to an anti-aging, nourishing, and antioxidant composition containing extracts of olive oil and camellia seed, and a method for preparing the same. Background Technology

[0002] Skin aging is a complex biological process driven by both endogenous (such as genetics and hormonal changes) and exogenous (such as ultraviolet radiation, pollution, and unhealthy lifestyle habits) factors. Its clinical manifestations include, but are not limited to: the formation of fine lines and wrinkles, decreased skin elasticity, loss of firmness, impaired skin barrier function, reduced stratum corneum moisture content, dull skin tone, and pigmentation. From the perspective of molecular mechanisms, skin aging mainly involves the following core pathways: (1) Oxidative stress: External environmental factors (especially ultraviolet radiation) induce the production of excessive reactive oxygen species (ROS) in skin cells, which attack cell membrane lipids, proteins (such as collagen and elastin) and DNA, leading to cell function damage and extracellular matrix degradation; (2) Chronic subclinical inflammation ("inflammatory aging"): Continuous oxidative stress and external stimuli can activate inflammatory pathways such as NF-κB, leading to the continuous low-level release of pro-inflammatory factors (such as IL-1β, IL-6, TNF-α), which in turn activates matrix metalloproteinases (MMPs), accelerates the decomposition of collagen and elastin fibers, and damages the skin barrier; (3) Reduced extracellular matrix synthesis and accelerated degradation: Fibroblast function declines, the ability to synthesize type I and type III collagen, elastin and hyaluronic acid decreases, and at the same time, MMP activity is upregulated, leading to the collapse of the skin support structure; (4) Weakened skin barrier function: Reduced synthesis of stratum corneum lipids (such as ceramides), increased transepidermal water loss (TEWL), and decreased skin moisturizing ability and resistance to external stimuli.

[0003] Currently, there are many active ingredients in anti-aging cosmetics on the market, which can be mainly divided into the following categories: (1) Antioxidants: such as vitamin C, vitamin E, coenzyme Q10, ferulic acid, etc., which aim to neutralize free radicals and reduce oxidative damage; (2) Cell communication factors and signal peptides: such as retinol and its derivatives, peptides, which aim to stimulate collagen production or inhibit its degradation; (3) Moisturizing and barrier repair ingredients: such as ceramides, cholesterol, fatty acids, which aim to replenish intercellular lipids and repair the skin barrier; (4) Plant extracts: such as green tea extract, resveratrol, centella asiatica extract, etc., which usually have multi-target effects, such as anti-oxidation and anti-inflammation. However, existing technologies still have obvious limitations: First, most products focus on a single or a few aging pathways. For example, potent antioxidants may have limited effects on inflammatory pathways or barrier repair, while simple moisturizers cannot effectively address deep collagen loss. This "lone wolf" strategy is difficult to comprehensively and synergistically combat the multi-factor intertwined skin aging network. Secondly, some highly effective ingredients (such as high-concentration retinol and fruit acids) are often accompanied by side effects such as skin irritation, dryness, and peeling, limiting their long-term use, especially for people with sensitive skin. Furthermore, although many plant extracts are claimed to have anti-aging effects, they are mostly used alone or in simple combinations, lacking scientific research on formulations based on clear synergistic mechanisms. This results in insignificant cumulative effects and may even reduce stability or efficacy due to ingredient conflicts.

[0004] Therefore, there is an urgent need in this field to develop a novel skincare composition that can achieve synergistic effects on multiple targets and pathways through the scientific combination of various plant active ingredients. This composition can effectively combat aging while also possessing excellent anti-inflammatory and skin barrier repair functions. Furthermore, it should be gentle and suitable for long-term use, in order to solve the technical problems of existing anti-aging products having single efficacy, potential irritation, or unclear synergistic effects. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the defects of existing anti-aging cosmetics that mostly focus on a single pathway, have insufficient synergistic effects, or may be accompanied by irritation, and to provide an anti-aging, nourishing, and antioxidant composition containing olive and camellia seed extracts and its preparation method.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides an anti-aging, nourishing, and antioxidant composition containing extracts of olive oil and camellia seed, characterized in that it comprises the following components in parts by weight: Hydrolyzed olive leaf extract: 3-5 parts; Camellia seed extract: 1-3 parts; White Flower Patrinia Extract: 7-9 parts; Horsetail extract: 6-8 parts.

[0007] Hydrolyzed olive leaf extract, rich in oleuropein and other polyphenolic compounds, serves as a core antioxidant and anti-inflammatory component. It can directly scavenge free radicals, inhibit ROS generation, and downregulate inflammatory signaling pathways such as NF-κB, thereby reducing oxidative stress and inflammation at their source, protecting skin cells from damage, and creating a favorable microenvironment for subsequent repair and regeneration.

[0008] Camellia seed extract: Rich in active substances such as tea polyphenols and tea saponins, it has strong antioxidant capabilities, helps to eliminate free radicals, and inhibits tyrosinase activity, thus helping to brighten skin tone. At the same time, its oil components have excellent moisturizing properties, improving dry skin and synergistically enhancing skin hydration and radiance.

[0009] Patrinia scabiosifolia extract: The main active ingredients are iridoids and flavonoids, which have significant anti-inflammatory, soothing, and calming effects. It can effectively inhibit histamine release and the production of various pro-inflammatory mediators, improve skin redness and sensitivity caused by inflammation or microcirculation disorders, thereby interrupting the vicious cycle of "inflammatory aging" and providing a stable environment for skin repair.

[0010] Horsetail extract: Rich in silicic acid, flavonoids, and saponins. Silicon is an important component of the stratum corneum and connective tissue of the skin. The soluble silicic acid in horsetail extract can be absorbed and utilized by the skin, participating in keratinization and collagen synthesis, helping to promote keratinocyte differentiation and enhance the integrity of the skin's physical barrier. Its flavonoid components also possess antioxidant and anti-inflammatory properties. This component focuses on strengthening the skin barrier function, improving the skin's water retention capacity and resistance to external stimuli, and consolidating anti-aging results.

[0011] The four components mentioned above, when combined in a specific mass ratio, produced a significant synergistic effect.

[0012] Preferably, the composition comprises the following components in parts by weight: Hydrolyzed olive leaf extract: 4 parts; Camellia seed extract: 2 parts; White Flower Patrinia Extract: 8 parts; Horsetail extract: 7 parts.

[0013] In a second aspect, the present invention provides the use of the composition as described in the first aspect in the preparation of cosmetics having anti-aging, nourishing, and antioxidant effects.

[0014] Preferably, the dosage form of the cosmetic is any one of lotion, cream, serum, mask, or spray.

[0015] Thirdly, the present invention provides an essence with anti-aging, nourishing and antioxidant effects, wherein the essence contains the anti-aging, nourishing and antioxidant composition containing olive and camellia seed extracts as described in the first aspect.

[0016] Preferably, the serum further includes skin conditioning agents, surfactants, thickeners, preservatives, and solvents.

[0017] More preferably, the skin conditioning agent includes at least one of glycerin, panthenol, allantoin, sodium hyaluronate, and trehalose; The surfactant includes at least one of hydrogenated lecithin, ammonium acryloyldimethyl taurate / VP copolymer, and sodium polyacryloyldimethyl taurate; The thickener includes at least one of sclerotinia gum, xanthan gum, acrylate / C10-30 alkanol acrylate crosspolymer, and carbomer; The preservative includes at least one of the following: phenoxyethanol, 1,2-hexanediol, 1,2-pentanediol, and phenoxyethanol. The solvent is deionized water.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows: Synergistic Effect of Multiple Targets to Form a Closed Loop for Anti-Aging Repair: This invention is not a simple superposition of four plant extracts, but a scientific combination based on the mechanisms of action of each component, forming a synergistic network from "source inhibition - mid-layer repair - bottom layer revitalization". Specifically, the synergistic mechanism is as follows: Hydrolyzed olive leaf extract acts as a vanguard, powerfully scavenging free radicals and inhibiting the initiation of inflammation, creating a stable "low-oxidation - low-inflammation" environment for subsequent repair; Camellia seed extract and Patrinia scabiosifolia extract work synergistically to strengthen the antioxidant defense line on the one hand, and on the other hand, to focus on inhibiting existing inflammatory factors, soothing the skin and breaking the chain of inflammation accelerating aging; Equisetum hyemale extract, in the favorable environment created by the above components, efficiently promotes keratin formation and barrier lipid synthesis, repairing the damaged skin physical barrier. Conversely, a healthy barrier can reduce oxidation and inflammation caused by external stimuli, thereby reducing the oxidative and inflammatory stress on the skin, assisting hydrolyzed olive leaf extract and Patrinia scabiosifolia extract in exerting their repairing effects, forming a positive repair cycle. Meanwhile, the moisturizing properties of camellia seed extract and the barrier-repairing properties of horsetail extract work together to enhance skin hydration. Good hydration is the foundation for normal cell metabolism and collagen synthesis, thus indirectly promoting anti-aging effects.

[0019] This composition, through the aforementioned synergistic mechanism, simultaneously achieves multiple effects including anti-oxidation, anti-inflammation, promoting barrier repair, and stimulating collagen regeneration, comprehensively improving various signs of aging such as wrinkles, sagging, dryness, and dullness. All ingredients are plant-derived and mildly formulated. Human skin patch tests show no irritation, making it suitable for long-term use on various skin types, including sensitive skin. Detailed Implementation

[0020] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0021] Hydrolyzed olive leaf extract: Guangzhou Xincheng Meike Biotechnology Co., Ltd.; Camellia seed extract: Guangzhou Boyi Trading Co., Ltd.; White Flower Patrinia Extract: Guangzhou Boyi Trading Co., Ltd.; Horsetail extract: Guangzhou Boyi Trading Co., Ltd.; All other raw materials are commercially available cosmetic grade raw materials.

[0022] Preparation of the combination of examples and comparative examples Example 1 Composed of the following components by mass: Hydrolyzed olive leaf extract: 4 parts; Camellia seed extract: 2 parts; White Flower Patrinia Extract: 8 parts; Horsetail extract: 7 parts; Preparation method: Place the above components in a mixing container and stir until they are evenly mixed to obtain the example. Seal and store in the dark.

[0023] Example 2 Composed of the following components by mass: Hydrolyzed olive leaf extract: 3 parts; Camellia seed extract: 3 parts; White Flower Patrinia Extract: 9 parts; Horsetail extract: 6 parts; The preparation method is the same as in Example 1.

[0024] Example 3 Composed of the following components by mass: Hydrolyzed olive leaf extract: 5 parts; Camellia seed extract: 1 part; White Flower Patrinia Extract: 7 parts; Horsetail extract: 8 parts; The preparation method is the same as in Example 1.

[0025] Comparative Example 1 Compared with Example 1, hydrolyzed olive leaf extract is missing. The missing mass fraction is made up by camellia seed extract, patrinia scabiosifolia extract and horsetail extract in a mass ratio of 2:8:7. The other components, component mass fractions and preparation methods are the same as in Example 1.

[0026] Comparative Example 2 Compared with Example 1, camellia seed extract is missing. The missing mass is made up by hydrolyzed olive leaf extract, white patrinia extract and horsetail extract in a mass ratio of 4:8:7. The other components, the mass of the components and the preparation method are the same as in Example 1.

[0027] Comparative Example 3 Compared with Example 1, the extract of Patrinia scabiosifolia is missing. The missing mass is made up by hydrolyzed olive leaf extract, camellia seed extract and horsetail extract in a mass ratio of 4:2:7. The other components, the mass of the components and the preparation method are the same as in Example 1.

[0028] Comparative Example 4 Compared with Example 1, the missing component is Equisetum arvense extract. The missing mass is made up by hydrolyzed olive leaf extract, camellia seed extract and patrinia scabiosifolia extract in a mass ratio of 4:2:8. The other components, component mass parts and preparation methods are the same as in Example 1.

[0029] Comparative Example 5 Composed of the following components by mass: Hydrolyzed olive leaf extract: 1 part; Camellia seed extract: 8 parts; White Flower Patrinia Extract: 3 parts; Horsetail extract: 6 parts; The preparation method is the same as in Example 1.

[0030] Preparation of serum Essence 1: The formula, by mass percentage, is as follows: 5.0% of the composition prepared in Example 1, 5.0% glycerin, 1.0% panthenol, 0.1% sodium hyaluronate, 0.1% xanthan gum, 0.2% acrylate / C10-30 alkanol acrylate crosspolymer, 0.1% allantoin, 0.5% phenoxyethanol, 0.2% p-hydroxyacetophenone, and 1.0% 1,2-hexanediol. The pH is adjusted to 5.5-6.0 with 10% sodium hydroxide aqueous solution, and deionized water is added to bring the volume to 100%. In preparation, xanthan gum and acrylate polymers are first dispersed separately in 1 / 3 of deionized water to swell and obtain gels for later use. Glycerin, panthenol, allantoin, sodium hyaluronate, preservatives, and the composition are dissolved in the remaining water and stirred until homogeneous to form an aqueous phase. The two gels are slowly added to the aqueous phase under stirring, homogenized, and the pH is adjusted. The mixture is then stirred until homogeneous to obtain the final product.

[0031] Serum 2: The only difference from Serum 1 is that it uses the same mass percentage as Example 2 to replace Example 1.

[0032] Serum 3: The only difference from Serum 1 is that it uses the same mass percentage as Example 3 to replace Example 1.

[0033] Serum 4: The only difference from serum 1 is that the mass percentage of Example 1 is 1%.

[0034] Serum 5: The only difference from serum 1 is that the mass percentage of Example 1 is 6%.

[0035] Serum ①: The only difference from serum 1 is that it uses the same mass percentage as comparative example 1 to replace example 1.

[0036] Serum ②: The only difference from serum 1 is that it uses the same mass percentage as comparative example 2 to replace example 1.

[0037] Serum ③: The only difference from serum 1 is that it uses the same mass percentage as comparative example 3 to replace example 1.

[0038] Serum ④: The only difference from Serum 1 is that Comparative Example 4 is used to replace Example 1 with the same mass percentage.

[0039] Serum ⑤: The only difference from serum 1 is that it uses the same mass percentage as Comparative Example 5 to replace Example 1.

[0040] Blank Serum: The only difference from Serum 1 is that it does not contain any of Example 1 (5.0% by weight of Example 1 is removed, and this portion is made up using deionized water).

[0041] Efficacy verification Test Example 1: Assay to Promote Collagen Production in Human Dermal Fibroblasts Test samples: The compositions of Examples 1-3 and Comparative Examples 1-5 were prepared into test sample working solutions of 0.1 mg / mL under aseptic conditions using cell culture.

[0042] Experimental Procedure: Healthy human dermal fibroblasts (HDF) were seeded at a density of 1 × 10⁴ cells per well in 96-well plates and cultured at 37°C with 5% CO₂ for 24 hours to allow cell adhesion. The old culture medium was discarded. The experimental groups were treated with the added working solution of the test sample, while the blank control group was treated with an equal volume of complete culture medium without the sample. Each group had 6 replicates. After culturing for another 72 hours, the cell supernatant was collected. The hydroxyproline content in the supernatant of each well was determined according to the instructions of the hydroxyproline assay kit (purchased from Nanjing Jiancheng Biotechnology Institute). Hydroxyproline is a characteristic amino acid of collagen, and its content can indirectly reflect the amount of collagen synthesized.

[0043] Using the hydroxyproline content of the blank control group as a baseline (set as 100%), the relative upregulation rate of collagen in each experimental sample group was calculated.

[0044] Relative upregulation rate of collagen (%) = [(Hydroxyproline content in sample group - Hydroxyproline content in blank control group) / Hydroxyproline content in blank control group] × 100% The experimental results are shown in Table 1.

[0045] Table 1. Effects of the Examples on Collagen Secretion Example 1 37.2 Example 2 34.5 Example 3 35.1 Comparative Example 1 19.4 Comparative Example 2 22.5 Comparative Example 3 17.3 Comparative Example 4 15.1 Comparative Example 5 27.6 This experiment evaluated the promoting effect of various compositions on collagen synthesis in human dermal fibroblasts (HDF) by measuring the hydroxyproline content in cell supernatants. The results showed that within the ratio range defined in this invention (Examples 1-3), all compositions exhibited excellent collagen-promoting effects, with the preferred ratio (Example 1, 4:2:8:7) showing the most significant effect, achieving a relative upregulation rate of 37.2% in collagen. This excellent effect stems from the synergistic effect of the four plant extracts: hydrolyzed olive leaf extract, through its potent antioxidant and anti-inflammatory properties, created a stable microenvironment of "low oxidation-low inflammation" for cells, mitigating the inhibition of fibroblast synthesis function by external stress; Patrinia scabiosifolia extract further inhibited inflammatory pathways, while the antioxidant aid and moisturizing properties of Camellia japonica seed extract helped maintain cell activity and metabolism. Under these favorable conditions, the silicic acid and other components contained in Equisetum hyemale extract contributed to the healthy construction of the extracellular matrix. The four components synergistically promoted the collagen synthesis function of fibroblasts.

[0046] Data from Comparative Examples 1-4 validated the necessity of this synergistic network. Comparative Example 1 (lacking hydrolyzed olive leaf extract) lacked core antioxidant and anti-inflammatory agents, thus failing to effectively scavenge free radicals and inhibit inflammation initiation, resulting in a significant decrease in collagen production promotion (19.4%). Comparative Example 3 (lacking Patrinia scabiosifolia extract) showed a weakened anti-inflammatory component, while Comparative Example 4 (lacking Equisetum hyemale extract) lacked barrier repair and matrix construction components, both leading to interrupted synergistic pathways and poor efficacy (17.3% and 15.1%, respectively). Comparative Example 2 (lacking Camellia japonica seed extract) showed a better effect (22.5%) than the comparative examples lacking other core components, but significantly lower than Example 1, indicating that its antioxidant assistance and moisturizing effects positively contributed to the synergistic effect. Although the effect of Comparative Example 5 (ratio 1:8:3:6, imbalanced ratio) (27.6%) was better than that of the single missing comparative example, confirming the partial effect of the coexistence of the four components, it was still significantly lower than that of Examples 1-3 with the specific ratio of the present invention. This proves that the ratio range defined by the present invention is not obvious and critical for achieving the best synergistic effect.

[0047] Test Example 2: Assay to Inhibit the Release of the Inflammatory Factor IL-6 Test samples: The compositions of Examples 1-3 and Comparative Examples 1-5 were prepared into test sample working solutions of 0.1 mg / mL under aseptic conditions using cell culture.

[0048] Experimental Procedure: Human immortalized keratinocytes (HaCaT) were used in the experiment. Cells were seeded in 24-well plates and cultured to 70-80% confluence. A normal control group (culture medium only), a model control group (LPS stimulation), and a sample group (LPS stimulation + test samples) were set up. Except for the normal control group, all other groups were first stimulated with culture medium containing 1 μg / mL lipopolysaccharide (LPS) for 1 hour to establish an inflammation model. Then, the model control group was replaced with fresh culture medium, and the sample group was replaced with culture medium containing 0.05% (v / v) of different test sample working solutions. After culturing for another 24 hours, the cell supernatant was collected. The concentration of IL-6 in the supernatant was detected using a human interleukin-6 (IL-6) enzyme-linked immunosorbent assay (ELISA) kit (purchased from Shanghai Enzyme-Link Biotechnology Co., Ltd.).

[0049] IL-6 inhibition rate (%) = [1 - (IL-6 concentration in sample group - IL-6 concentration in normal control group) / (IL-6 concentration in model control group - IL-6 concentration in normal control group)] × 100% The experimental results are shown in Table 2.

[0050] Table 2 Effect of the composition on the inhibition rate of IL-6 Example 1 29.7 Example 2 24.2 Example 3 26.5 Comparative Example 1 15.4 Comparative Example 2 15.2 Comparative Example 3 16.7 Comparative Example 4 13.9 Comparative Example 5 19.8 This study evaluated the inhibitory effect of various compositions on the release of the key pro-inflammatory factor IL-6 using an LPS-induced HaCaT cell inflammation model. The composition in Example 1 showed the highest IL-6 inhibition rate (29.7%), demonstrating a synergistic anti-inflammatory effect. The mechanism is that hydrolyzed olive leaf extract (rich in oleuropein) and *Patrinia scabiosifolia* extract (rich in iridoids and flavonoids) form the core of the anti-inflammatory synergy. Hydrolyzed olive leaf extract can inhibit the activation of key inflammatory signaling pathways such as NF-κB upstream, while *Patrinia scabiosifolia* extract can effectively inhibit the release of histamine and various inflammatory mediators downstream. Together, they disrupt the inflammatory cascade from different points. The antioxidant effect of camellia seed extract helps reduce inflammation induced by oxidative stress, while horsetail extract indirectly enhances the cell's resistance to external stimuli (such as LPS) by strengthening the cell barrier.

[0051] The comparative data strongly support this synergistic mechanism. The anti-inflammatory effects of Comparative Example 1 (lacking hydrolyzed olive leaf extract) and Comparative Example 3 (lacking Patrinia scabiosifolia extract) were most significantly impaired (inhibition rates of 15.4% and 16.7%, respectively), indicating that these two components are indispensable core components in the anti-inflammatory synergy. The effects of Comparative Example 2 (lacking camellia seed extract) and Comparative Example 4 (lacking horsetail extract) (15.2% and 13.9%, respectively) were also significantly reduced, indicating that their auxiliary antioxidant and barrier support functions are important for achieving optimal anti-inflammatory effects. Although the inhibition rate of Comparative Example 5 (imbalanced ratio) (19.8%) was higher than that of the comparative examples lacking the core components, it was far lower than that of Example 1, further demonstrating that the specific formulation of the present invention is crucial for achieving the optimized anti-inflammatory synergistic effect.

[0052] Test Example 3: Security Test This invention experimentally verifies the biocompatibility of serums 1-5, serums ①-⑤, and a blank serum.

[0053] The irritation of cosmetics was evaluated using the 2015 Cosmetic Safety Technical Specifications as a reference standard. The test method was a skin patch test, and the test was conducted on subjects aged 16-65 years who were randomly distributed. Each subject was assigned to all test samples, for a total of 33 subjects.

[0054] Test samples: serums 1-5, serums ①-⑤, blank serum.

[0055] Test Method: The test substance is placed in a patch applicator at a dosage of 0.020-0.025 g. The back of the subject is divided into test areas according to the number of test samples, with each test area corresponding to one test sample. The patch applicator containing the test substance is then covered with non-irritating adhesive tape on the subject's back. Gently press the patch applicator with the palm of the hand to ensure even adhesion to the skin surface. This process is continued for 24 hours. The patch applicator is removed 30 minutes after the pressure marks have disappeared, and the skin reaction is observed. If the result is negative, repeat observations are performed at 24 and 48 hours after the patch test.

[0056] Evaluation criteria: Grade 0: Negative reaction; Grade 1: Suspicious reaction, with only slight erythema; Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules; Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area; Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0057] Test results: All subjects had negative skin reactions, indicating that the serum provided by this invention is safe and non-irritating.

[0058] Test Example 4: Human Efficacy Test Test samples: serums 1-5, serums ①-⑤, blank serum.

[0059] Subjects: Healthy female subjects aged 35-55 years with mild to moderate fine lines on their face, dry skin, and a self-perceived dull complexion were recruited. Subjects were randomly divided into groups according to the number of test samples, with each group corresponding to one test sample and 10 subjects in each group.

[0060] Experimental Methods: All subjects applied the designated product to their face after cleansing morning and evening for 8 weeks. Quantitative measurements were performed by the same experienced evaluator in a temperature- and humidity-controlled room using a professional skin analyzer before (D0) and after 8 weeks (D56). Measurement indicators included: 1) Wrinkle volume: The wrinkle volume (mm³) in the crow's feet area at the corner of the eye was analyzed using a rapid skin imaging system. The smaller the wrinkle volume, the better the skin condition. 2) Skin stratum corneum moisture content: The moisture content of the cheek area is measured using a skin moisture meter. The higher the moisture content, the better the skin condition. 3) Transepidermal water loss (TEWL): The TEWL meter is used to measure the cheek area to assess the skin barrier function. The lower the transepidermal water loss value, the better the skin condition. 4) Skin tone brightness: The ITA° angle of the forehead area is measured using a spectrophotometer. The larger the ITA° value, the brighter the skin tone.

[0061] Individual rate of change (%) = [(D56 measurement - D0 measurement) / D0 measurement] × 100% Average rate of change (%) = Sum of individual rates of change within the group / Number of individuals in the group The experimental results are shown in Table 3.

[0062] Table 3. Results of human efficacy tests for the serum. Serum 1 -18.5 32.4 -15.6 12.3 Serum 2 -16.8 30.1 -13.9 10.5 Serum 3 -17.2 29.5 -14.3 11.2 Serum 4 -9.5 18.1 -8.1 5.7 Serum 5 -20.1 35.2 -16.8 13.5 Serum ① -5.2 10.5 -4.3 3.1 Serum ② -7.1 12.8 -5.9 4.2 Serum ③ -4.8 9.7 -3.8 2.8 Essence ④ -6.5 11.6 -5.1 3.5 Essence ⑤ -12.4 22.3 -10.5 7.9 Blank Essence -2.1 5.8 -1.3 1.5 The human efficacy test lasted for 8 weeks, comprehensively evaluating the actual skincare effects of the product from four dimensions: anti-wrinkle, moisturizing, barrier repair, and skin brightening. Using serum 1 containing the complete synergistic composition of this invention (Example 1), the best overall improvement was achieved across all indicators, directly verifying the successful application of the multi-target synergistic mechanism of this composition on real human skin.

[0063] Serum 1 significantly reduced wrinkle volume by 18.5% and decreased TEWL value by 15.6%. This effect stems from a complete synergistic pathway: hydrolyzed olive leaf extract and white patrinia extract, through their antioxidant and anti-inflammatory effects, reduced the degradation of existing collagen by matrix metalloproteinases (MMPs) and alleviated the damage to the skin barrier caused by inflammation; horsetail extract directly promoted barrier lipid synthesis and keratinization, enhancing the skin's water retention and defense capabilities; a healthy barrier and low-inflammatory environment, in turn, created conditions for fibroblasts to synthesize new collagen (corresponding to the results of test example 1), thus achieving anti-wrinkle effects from both "reducing degradation" and "promoting regeneration," while simultaneously repairing the skin barrier.

[0064] Serum 1 increases the skin's stratum corneum moisture content by 32.4% and skin tone ITA value by 12.3%. The natural moisturizing properties of camellia seed extract and the barrier-repairing properties of horsetail extract work together to significantly enhance the skin's own moisturizing ability. At the same time, hydrolyzed olive leaf extract and camellia seed extract effectively eliminate free radicals, inhibit lipid peroxidation and pigmentation through their powerful antioxidant capabilities, thereby improving dull skin tone and achieving a brightening effect.

[0065] All serums (①-⑤) using incomplete or imbalanced compositions failed to reach the efficacy levels of serum 1. Serums ①, ③, and ④ (lacking hydrolyzed olive leaf, white patrinia, and horsetail extracts, respectively) showed the weakest performance in anti-wrinkle (corresponding to the lack of antioxidant / anti-inflammatory or barrier repair cores) and moisturizing / repair (corresponding to the lack of barrier cores). While serum ⑤ (imbalanced ratio) was more effective than the comparative products lacking certain components, it lagged behind serums 1-3 across the board. This again demonstrates, at the human trial level, the necessity and non-obviousness of the specific formulations described in this invention for achieving optimal efficacy in commercial products.

[0066] In summary, the composition of this invention, through the scientific formulation of four plant extracts, forms a synergistic network of anti-oxidation, anti-inflammation, repair, and nourishment. Its comprehensive and excellent anti-aging and repair effects have been demonstrated in both cell experiments and human trials, and it is highly safe, possessing outstanding industrial application value.

[0067] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-aging, nourishing, and antioxidant composition containing extracts of olive oil and camellia seed, characterized in that, Includes the following components by weight: Hydrolyzed olive leaf extract: 3-5 parts; Camellia seed extract: 1-3 parts; White Flower Patrinia Extract: 7-9 parts; Horsetail extract: 6-8 parts.

2. The composition according to claim 1, characterized in that, Includes the following components by weight: Hydrolyzed olive leaf extract: 4 parts; Camellia seed extract: 2 parts; White Flower Patrinia Extract: 8 parts; Horsetail extract: 7 parts.

3. The use of the composition according to claim 1 or 2 in the preparation of cosmetics with anti-aging, nourishing and antioxidant effects.

4. The application as described in claim 3, characterized in that, The cosmetic product can be any one of the following forms: lotion, cream, serum, mask, or spray.

5. A serum with anti-aging, nourishing, and antioxidant effects, characterized in that, The anti-aging, nourishing, and antioxidant composition comprising olive and camellia seed extracts as described in claim 1 or 2.

6. The essence as described in claim 5, characterized in that, It also includes skin conditioning agents, surfactants, thickeners, preservatives, and solvents.

7. The essence as described in claim 6, characterized in that, The skin conditioning agent includes at least one of glycerin, panthenol, allantoin, sodium hyaluronate, and trehalose.

8. The essence as described in claim 6, characterized in that, The surfactant includes at least one of hydrogenated lecithin, ammonium acryloyldimethyl taurate / VP copolymer, and sodium polyacryloyldimethyl taurate.

9. The essence as described in claim 6, characterized in that, The thickener includes at least one of sclerotinia gum, xanthan gum, acrylate / C10-30 alkanol acrylate crosspolymer, and carbomer.

10. The essence as described in claim 6, characterized in that, The preservative includes at least one of phenoxyethanol, 1,2-hexanediol, 1,2-pentanediol, and phenoxyethanol; the solvent is deionized water.