A whitening and freckle-removing essence containing eaglewood and a preparation method thereof

Abstract

The application provides a white spot removing essence containing eaglewood and a preparation method thereof, and relates to the technical field of essences. The essence is prepared from the following raw materials in parts by weight: 2 to 5 parts of eaglewood fermentation extract, 1 to 3 parts of melia toosendan leaf extract, 0.5 to 2 parts of radix cnidii root extract, 0.1 to 0.2 parts of rabdosia amabilis extract, 1 to 1.5 parts of ectoine, 1 to 5 parts of panthenol, 0.5 to 1 part of sodium hyaluronate, 2 to 3 parts of betaine, 3 to 5 parts of emulsifier, 0.1 to 2 parts of thickening agent, 0.1 to 0.3 part of preservative and 80 to 120 parts of water. The essence can remove DPPH free radicals and other free radicals, avoid the activation of tyrosinase by free radicals from the source, and synergistically inhibit the activity of tyrosinase by the combination of the eaglewood fermentation extract, the melia toosendan leaf extract and other raw materials, so as to reduce the generation and transfer of melanin in cells, and achieve the effects of whitening and white spot removal.

Description

Technical Field

[0001] This invention relates to the field of serum technology, and in particular to a whitening and spot-removing serum containing agarwood and its preparation method. Background Technology

[0002] As people's living standards improve, they pay more attention to their appearance. The saying "fair skin covers a hundred flaws" highlights the growing importance of fair skin as a standard of beauty. Consequently, products with whitening effects are gaining wider consumer attention, leading to increasing demand. Under conditions of ultraviolet radiation, hormonal fluctuations, and the accumulation of inflammatory factors, tyrosinase in the body is abnormally activated. Tyrosine in the melanocytes of the epidermal basal layer is oxidized to dopa (DOPA) by tyrosinase (TYR), and further oxidized to dopaquinone, ultimately forming melanin or pheomelanin, resulting in dull, darkened skin or the appearance of spots.

[0003] Currently, to achieve skin whitening and blemish-removing effects, substances such as niacinamide, arbutin, glycyrrhizin, and tranexamic acid are commonly used. However, while high-concentration chemical whitening products can achieve whitening effects in the short term, they are highly irritating to the skin, easily causing redness and stinging. Many natural active ingredients are relatively safe and also have certain whitening and blemish-removing effects; however, single ingredients are difficult to achieve good whitening and blemish-removing results, and most of these ingredients are also unstable, with poor absorption, making it difficult to fully exert their whitening and blemish-removing effects. Agarwood [Aquilaria sinensis (Lour.) Gilg] is rich in flavonoids, terpenes, and phenols, among other bioactive compounds, which can play a role in analgesia, antibacterial, anti-inflammatory, antioxidant, antitumor, and blood pressure lowering effects. Using it in whitening and blemish-removing essences can broaden its application range and improve its economic benefits. Summary of the Invention

[0004] In view of this, the present invention proposes a whitening and freckle-removing essence containing agarwood and its preparation method.

[0005] The technical solution of this invention is implemented as follows: A whitening and freckle-removing essence containing agarwood comprises the following ingredients in parts by weight: 2-5 parts fermented agarwood extract, 1-3 parts *Syzygium aromaticum* leaf extract, 0.5-2 parts *Angelica dahurica* root extract, 0.1-0.2 parts *Tetrapanax papyriferus* extract, 1-1.5 parts ectoine, 1-5 parts panthenol, 0.5-1 part sodium hyaluronate, 2-3 parts betaine, 3-5 parts emulsifier, 0.1-2 parts thickener, 0.1-0.3 parts preservative, and 80-120 parts water.

[0006] Furthermore, the preparation method of the agarwood fermentation extract is as follows: the agarwood raw material is crushed into agarwood powder, water and cellulase are added for enzymatic hydrolysis, the enzyme is inactivated to obtain the enzymatic hydrolysate, and then fermented with fermentation liquid and yeast / rice bran fermentation product, sterilized, centrifuged, filtered, and dried to obtain the agarwood fermentation extract.

[0007] Furthermore, the ratio of agarwood powder to water in liquid is 1:10~13 (g / mL); the amount of cellulase added is 0.5%~1.0% of the total mass of agarwood powder and water; the amount of fermentation liquid added is 0.8%~1.2% of the mass of the enzymatically hydrolyzed raw materials; and the amount of yeast / rice bran fermentation product added is 4%~6% of the mass of the enzymatically hydrolyzed raw materials.

[0008] Furthermore, the fermentation broth includes *Lactobacillus rhamnosus* and *Lactobacillus delbrueckii* in a mass ratio of 2-3:1; the viable count of *Lactobacillus rhamnosus* is 1×10⁻⁶. 8 -2×10 8 CFU / mL; the viable count of *Lactobacillus delbrueckii* was 1×10⁻⁶. 8 -2×10 8 CFU / mL.

[0009] Furthermore, the fermentation is carried out at a temperature of 30~37℃ and a stirring speed of 80~120r / min for 2~3 days.

[0010] Furthermore, the preparation method of the *Syzygium serratum* leaf extract is as follows: pulverize *Syzygium serratum* leaves to obtain *Syzygium serratum* leaf powder, soak the *Syzygium serratum* leaf powder in ethanol solution A, then perform a first ultrasonic extraction, filter, obtain filtrate and residue, perform a second ultrasonic extraction with ethanol solution B on the residue, filter, combine all filtrates, concentrate the filtrate, and dry to obtain the *Syzygium serratum* leaf extract.

[0011] Furthermore, the ratio of the *Syzygium aromaticum* leaves to the ethanol solution (g / mL) is 1:10~15; the soaking temperature is 40~50℃ and the soaking time is 2~3h; the ratio of the filter residue to the ethanol solution B (g / mL) is 1:6~8.

[0012] Furthermore, the temperature of the single ultrasonic extraction is 50~60℃, the power is 200~300W, and the time is 60~90min.

[0013] Furthermore, the temperature of the secondary ultrasonic extraction is 50~60℃, the power is 400~450W, and the time is 30~40min.

[0014] Furthermore, the emulsifier includes one or more of polyglycerol-4 oleate, cetearyl alcohol, and cetearyl glucoside.

[0015] Furthermore, the thickener includes one or more of xanthan gum, carrageenan, carbomer, carboxymethyl cellulose, and hydroxypropyl cellulose.

[0016] Furthermore, the preservative includes one or more of phenoxyethanol, ethylhexylglycerin, methylparaben, and p-hydroxyacetophenone.

[0017] The preparation method of a whitening and spot-removing essence containing agarwood includes the following steps: (1) Add panthenol, sodium hyaluronate, betaine, emulsifier, and thickener to water and stir to obtain a mixed solution; (2) Add agarwood fermentation extract, mountain jasmine leaf extract, white angelica root extract, tea jasmine extract, ectoine and preservative to the mixed solution and stir well to obtain the target whitening and spot-removing essence.

[0018] Furthermore, in step (1), the stirring temperature is 55~60℃ and the stirring speed is 80~100r / min.

[0019] Furthermore, in step (2), the stirring temperature is 40~45℃ and the stirring speed is 80~100r / min.

[0020] Compared with the prior art, the beneficial effects of the present invention are: This invention obtains agarwood fermentation extract through a specific method, and then combines it with other raw materials to eliminate free radicals such as DPPH free radicals. This prevents free radicals from activating tyrosinase and accelerating melanin production at the source. At the same time, the combination of raw materials such as agarwood fermentation extract, mountain jasmine leaf extract, white angelica root extract, and fragrant tea extract can synergistically inhibit tyrosinase activity, reduce the production and transfer of melanin in cells, and work in multiple ways to effectively reduce and improve skin dullness and the formation of age spots, thus achieving a whitening and spot-removing effect.

[0021] This invention, in preparing agarwood fermentation extract, utilizes a combination of *Lactobacillus rhamnosus* and *Lactobacillus delbrueckii*, along with the addition of yeast / rice bran fermentation products during fermentation. This approach yields small-molecule active ingredients, enhances their penetration and absorption, and improves the synergistic effect between the agarwood fermentation extract and other raw material components, resulting in superior whitening and blemish-removing effects. Raw materials such as ectoine, panthenol, and sodium hyaluronate promote the penetration and absorption of various effective ingredients, while also promoting normal epidermal cell renewal and preventing melanin accumulation.

[0022] This invention uses ethanol solutions of different concentrations to perform multiple ultrasonic extractions on the leaves of *Syzygium aromaticum*, which can better preserve the active substances in the leaves and thus improve their efficacy. Detailed Implementation

[0023] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0024] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0025] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0026] Lactobacillus rhamnosus was obtained from the Guangdong Provincial Microbial Culture Collection Center, with the code GDMCC NO.1.1732; Lactobacillus delbrueckii was obtained from the Guangdong Provincial Microbial Culture Collection Center, named Lactobacillus delbrueckii subsp. bulgaricus, with the code GDMCC NO.1.1801; Lactobacillus plantarum was obtained from the Guangdong Provincial Microbial Culture Collection Center, with the code GDMCC NO.1.1516.

[0027] The root extract of Peucedanum praeruptorum was obtained from Xi'an Huilin Biotechnology Co., Ltd.; the extract of Isodon japonica was obtained from Shanyang Lianfeng Biotechnology Co., Ltd.; and the yeast / rice bran fermentation product was obtained from Shanghai Dingyun Chemical Trading Co., Ltd.

[0028] The cellulase activity is 20,000 U / g.

[0029] Example 1 A whitening and freckle-removing essence containing agarwood comprises the following ingredients in parts by weight: 5 parts fermented agarwood extract, 3 parts *Syzygium aromaticum* leaf extract, 2 parts *Angelica dahurica* root extract, 0.2 parts *Gnaphalium affine* extract, 1 part ectoine, 4 parts panthenol, 0.5 parts sodium hyaluronate, 3 parts betaine, 3 parts emulsifier, 1 part thickener, 0.1 part preservative, and 80 parts purified water; wherein the emulsifier is composed of cetearyl alcohol and cetearyl glucoside in a mass ratio of 1:2; the thickener is xanthan gum; and the preservative is composed of phenoxyethanol and ethylhexylglycerin in a mass ratio of 9:1.

[0030] The preparation method of agarwood fermentation extract is as follows: Agarwood raw material is crushed into agarwood powder, and then mixed with water at a ratio of 1:10 (g / mL). 1.0% (by mass) of cellulase (based on the total mass of agarwood powder and water) is added and enzymatically hydrolyzed at 50℃ for 3 hours. The enzyme is then inactivated to obtain the enzymatically hydrolyzed raw material. Fermentation broth and yeast / rice bran fermentation product are added and mixed thoroughly. Fermentation is carried out at 37℃ and a stirring speed of 80 r / min for 2 days. The mixture is then sterilized, centrifuged, filtered, and dried to obtain the agarwood fermentation extract. The amount of fermentation broth added is 1.2% of the mass of the enzymatically hydrolyzed raw material; the amount of yeast / rice bran fermentation product added is 6% of the mass of the enzymatically hydrolyzed raw material; the fermentation broth includes *Lactobacillus rhamnosus* and *Lactobacillus delbrueckii* at a mass ratio of 2:1; the viable count of *Lactobacillus rhamnosus* is 2 × 10⁻⁶. 8 CFU / mL; the viable count of *Lactobacillus delbrueckii* was 2 × 10⁻⁶. 8 CFU / mL.

[0031] The preparation method of *Syzygium aromaticum* leaf extract is as follows: *Syzygium aromaticum* leaves are pulverized to obtain *Syzygium aromaticum* leaf powder. The *Syzygium aromaticum* leaf powder is soaked in 85% ethanol solution A at a material-to-liquid ratio of 1:15 g / mL at 50℃ for 3 hours. Then, it is ultrasonically extracted at 50℃ and 300W for 90 minutes. After filtration, filtrate and residue are obtained. The residue is ultrasonically extracted with 50% ethanol solution B at a material-to-liquid ratio of 1:6 g / mL at 60℃ and 400W for 60 minutes. After filtration, all filtrates are combined, concentrated, and dried to obtain *Syzygium aromaticum* leaf extract.

[0032] The preparation method of a whitening and spot-removing essence containing agarwood includes the following steps: (1) Add panthenol, sodium hyaluronate, betaine, emulsifier, and thickener to water and stir at 80 r / min at 60℃ to obtain a mixed solution; (2) Cool the mixed solution to 40°C and add agarwood fermentation extract, mountain jasmine leaf extract, white angelica root extract, tea jasmine extract, ectoine and preservative to the mixed solution. Stir evenly at 150 r / min to obtain the target essence.

[0033] Example 2 A whitening and freckle-removing essence containing agarwood comprises the following ingredients in parts by weight: 4 parts agarwood fermentation extract, 1 part *Aquilaria sinensis* leaf extract, 1 part *Angelica dahurica* root extract, 0.1 part *Gnaphalium affine* extract, 1.5 parts ectoine, 5 parts panthenol, 1 part sodium hyaluronate, 2 parts betaine, 5 parts emulsifier, 2 parts thickener, 0.3 parts preservative, and 80 parts purified water; wherein the emulsifier is composed of polyglycerol-4 oleate, cetearyl alcohol, and cetearyl glucoside in a mass ratio of 1:1:1; the thickener is carbomer; and the preservative is phenoxyethanol.

[0034] The preparation methods for the fermented agarwood extract and the leaf extract of Rhododendron molle are the same as in Example 1.

[0035] The preparation method of a whitening and spot-removing essence containing agarwood includes the following steps: (1) Add panthenol, sodium hyaluronate, betaine, emulsifier, and thickener to water and stir at 100 r / min at 55℃ to obtain a mixed solution; (2) Cool the mixed solution to 40°C and add agarwood fermentation extract, mountain jasmine leaf extract, white angelica root extract, tea jasmine extract, ectoine and preservative to the mixed solution. Stir evenly at 120r / min to obtain the target essence.

[0036] Example 3 A whitening and freckle-removing essence containing agarwood comprises the following ingredients in parts by weight: 2 parts agarwood fermentation extract, 2 parts *Syzygium aromaticum* leaf extract, 0.5 parts *Angelica dahurica* root extract, 0.15 parts *Gnaphalium affine* extract, 1.2 parts ectoine, 3 parts panthenol, 0.8 parts sodium hyaluronate, 3 parts betaine, 4 parts emulsifier, 0.1 parts thickener, 0.2 parts preservative, and 90 parts purified water; wherein the emulsifier is cetearyl glucoside; the thickener is composed of carrageenan and carboxymethyl cellulose in a mass ratio of 1:1; and the preservative is composed of phenoxyethanol and ethylhexylglycerin in a mass ratio of 9:1.

[0037] The preparation methods for the fermented agarwood extract and the leaf extract of Rhododendron molle are the same as in Example 1.

[0038] The preparation method of a whitening and spot-removing essence containing agarwood includes the following steps: (1) Add panthenol, sodium hyaluronate, betaine, emulsifier, and thickener to water and stir at 90 r / min at 60℃ to obtain a mixed solution; (2) Cool the mixed solution to 45°C and add agarwood fermentation extract, mountain jasmine leaf extract, white angelica root extract, tea jasmine extract, ectoine and preservative to the mixed solution. Stir evenly at 1350 r / min to obtain the target essence.

[0039] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the essence does not contain agarwood fermentation extract, and the missing amount is made up by purified water.

[0040] A whitening and freckle-removing essence containing agarwood comprises the following ingredients in parts by weight: 3 parts of *Syzygium aromaticum* leaf extract, 2 parts of *Angelica dahurica* root extract, 0.2 parts of *Tetrapanax papyriferus* extract, 1 part of ectoine, 4 parts of panthenol, 0.5 parts of sodium hyaluronate, 3 parts of betaine, 3 parts of emulsifier, 1 part of thickener, 0.1 part of preservative, and 85 parts of purified water.

[0041] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the essence does not contain Sedum sarmentosum leaf extract, and the missing amount is made up by purified water.

[0042] A whitening and freckle-removing essence containing agarwood comprises the following ingredients in parts by weight: 5 parts agarwood fermentation extract, 2 parts white angelica root extract, 0.2 parts jasmine extract, 1 part ectoine, 4 parts panthenol, 0.5 parts sodium hyaluronate, 3 parts betaine, 3 parts emulsifier, 1 part thickener, 0.1 part preservative, and 83 parts purified water.

[0043] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that the essence does not contain the root extract of Angelica dahurica, and the missing amount is made up by purified water.

[0044] A whitening and freckle-removing essence containing agarwood comprises the following ingredients in parts by weight: 5 parts agarwood fermentation extract, 3 parts mountain agarwood leaf extract, 0.2 parts fragrant tea extract, 1 part ectoine, 4 parts panthenol, 0.5 parts sodium hyaluronate, 3 parts betaine, 3 parts emulsifier, 1 part thickener, 0.1 part preservative, and 82 parts purified water.

[0045] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that the essence does not contain herb extract, and the missing amount is made up by purified water.

[0046] A whitening and freckle-removing essence containing agarwood comprises the following ingredients in parts by weight: 5 parts agarwood fermentation extract, 3 parts mountain agarwood leaf extract, 2 parts white angelica root extract, 1 part ectoine, 4 parts panthenol, 0.5 parts sodium hyaluronate, 3 parts betaine, 3 parts emulsifier, 1 part thickener, 0.1 part preservative, and 80.2 parts purified water.

[0047] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that no yeast / rice bran fermentation products were added during the preparation of the agarwood fermentation extract.

[0048] The preparation method of agarwood fermentation extract is as follows: Agarwood raw material is crushed into agarwood powder, and then mixed with water at a ratio of 1:10 (g / mL). 1.0% (by mass) of cellulase (based on the total mass of agarwood powder and water) is added and enzymatically hydrolyzed at 50℃ for 3 hours. The enzyme is then inactivated to obtain the hydrolyzed raw material. Fermentation broth is added and mixed thoroughly. Fermentation is carried out at 37℃ with a stirring speed of 80 r / min for 2 days. The mixture is then sterilized, centrifuged, filtered, and dried to obtain the agarwood fermentation extract. The amount of fermentation broth added is 1.2% of the mass of the hydrolyzed raw material. The fermentation broth includes *Lactobacillus rhamnosus* and *Lactobacillus delbrueckii* in a mass ratio of 2:1. The viable count of *Lactobacillus rhamnosus* is 2 × 10⁻⁶. 8CFU / mL; the viable count of *Lactobacillus delbrueckii* was 2 × 10⁻⁶. 8 CFU / mL.

[0049] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that the fermented agarwood extract was replaced with an ethanol extract of agarwood.

[0050] The preparation method of agarwood ethanol extract is as follows: Agarwood raw material is crushed into agarwood powder, and the powder of mountain agarwood round leaves is soaked in ethanol solution A with a mass concentration of 85% at 50℃ for 3 hours at a material-liquid ratio of 1:10 g / mL. Then, it is refluxed and extracted 3 times, with each extraction time being 60 min. All filtrates are collected, the filtrates are concentrated and dried to obtain agarwood ethanol extract.

[0051] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that the fermentation liquid used for the agarwood fermentation extract was changed, and the fermentation liquid composed of Lactobacillus rhamnosus and Lactobacillus delbrueckii was replaced with an equal amount of Lactobacillus plantarum.

[0052] The preparation method of agarwood fermentation extract is as follows: Agarwood raw material is crushed into agarwood powder, then mixed with water at a ratio of 1:10 (g / mL). 1.0% (by weight of the total mass of agarwood powder and water) of cellulase is added and hydrolyzed at 50℃ for 3 hours. The enzyme is then inactivated to obtain the hydrolyzed raw material. Fermentation broth and yeast / rice bran fermentation product are added and mixed thoroughly. Fermentation is carried out at 37℃ and a stirring speed of 80 r / min for 2 days. The mixture is then sterilized, centrifuged, filtered, and dried to obtain the agarwood fermentation extract. The amount of fermentation broth added is 1.2% of the mass of the hydrolyzed raw material; the amount of yeast / rice bran fermentation product added is 3% of the mass of the hydrolyzed raw material; the fermentation broth is *Lactobacillus plantarum*; and the viable count of *Lactobacillus plantarum* is 2 × 10⁻⁶. 8 CFU / mL.

[0053] Performance test results: 1. DPPH free radical scavenging test Accurately weigh 2.0 mg of DPPH powder and dissolve it in 50 mL of anhydrous ethanol to obtain a DPPH solution for later use. Take the essence prepared in the examples and comparative examples and dilute it with anhydrous ethanol to a concentration of 5 mg / mL as a test sample solution. Add 100 μL of DPPH solution and then 100 μL of the test sample solution to a 96-well plate and incubate at 37°C in the dark for 30 min to obtain the sample group solution. Incubate 100 μL of DPPH solution and 100 μL of anhydrous ethanol under the same conditions to obtain the blank group solution. Incubate 100 μL of anhydrous ethanol and 100 μL of the test sample solution under the same conditions to obtain the control group solution. Use an ELISA reader to measure the absorbance of the blank group solution, control group solution and sample group solution at a wavelength of 517 nm. Calculate the DPPH free radical scavenging rate according to the formula. The results are shown in Table 1 below.

[0054] DPPH radical scavenging rate (%) = [A - (BC) / A × 100%; In the formula, A is the absorbance of the blank solution, B is the absorbance of the sample solution, and C is the absorbance of the control solution.

[0055] 2. Tyrosinase activity inhibition test The serums prepared in the examples and comparative examples were diluted with a phosphate buffer at pH 6.8 to prepare sample solutions with a concentration of 5 mg / mL. The inhibition rate of the serums prepared in the examples and comparative examples on tyrosinase activity was tested according to T / SHRH 015-2018. The results are shown in Table 1 below.

[0056] 3. The relative melanin secretion of the serum prepared according to the test examples and comparative examples in T / LNPC 017-2024 is shown in Table 1 below.

[0057] Table 1. Results of serum performance tests

[0058] As can be seen from the results in Table 1 above, the serums of Examples 1-3, compared with those of Comparative Examples 1-7, have superior effects in scavenging DPPH free radicals, inhibiting tyrosinase activity, and reducing melanin secretion, thus achieving a good whitening and spot-removing effect. The comparison between Example 1 and Comparative Examples 1-4 shows that the combination of four extracts—agarwood fermentation extract, *Ligusticum striatum* leaf extract, *Angelica dahurica* root extract, and *Gnaphalium affine* extract—achieves a good synergistic effect, enhancing the whitening and spot-removing effect of the serum. The comparison between Example 1 and Comparative Examples 5-7 shows that the present invention, by using *Lactobacillus rhamnosus* and *Lactobacillus delbrueckii* in combination, and simultaneously adding yeast / rice bran fermentation products during fermentation, can promote the interaction between the prepared agarwood fermentation extract and other raw materials, achieving a better whitening and spot-removing effect.

[0059] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A whitening and freckle-removing essence containing agarwood, characterized in that, The ingredients include the following parts by weight: 2-5 parts of fermented agarwood extract, 1-3 parts of *Syzygium aromaticum* leaf extract, 0.5-2 parts of *Angelica dahurica* root extract, 0.1-0.2 parts of *Tea japonica* extract, 1-1.5 parts of ectoine, 1-5 parts of panthenol, 0.5-1 part of sodium hyaluronate, 2-3 parts of betaine, 3-5 parts of emulsifier, 0.1-2 parts of thickener, 0.1-0.3 parts of preservative, and 80-120 parts of water.

2. The whitening and freckle-removing essence containing agarwood according to claim 1, characterized in that, The preparation method of the agarwood fermentation extract is as follows: the agarwood raw material is crushed into agarwood powder, water and cellulase are added for enzymatic hydrolysis, the enzyme is inactivated to obtain the enzymatic hydrolysate, and then fermented with fermentation liquid and yeast / rice bran fermentation product, sterilized, centrifuged, filtered, and dried to obtain the agarwood fermentation extract.

3. The whitening and freckle-removing essence containing agarwood according to claim 2, characterized in that, The ratio of agarwood powder to water in the liquid is 1:10~13 (g / mL); the amount of cellulase added is 0.5%~1.0% of the total mass of agarwood powder and water; the amount of fermentation liquid added is 0.8%~1.2% of the mass of the enzymatically hydrolyzed raw materials; and the amount of yeast / rice bran fermentation product added is 4%~6% of the mass of the enzymatically hydrolyzed raw materials.

4. The whitening and freckle-removing essence containing agarwood according to claim 2, characterized in that, The fermentation broth includes *Lactobacillus rhamnosus* and *Lactobacillus delbrueckii* in a mass ratio of 2-3:1; the viable count of *Lactobacillus rhamnosus* is 1×10⁻⁶. 8 -2×10 8 CFU / mL; the viable count of *Lactobacillus delbrueckii* was 1×10⁻⁶. 8 -2×10 8 CFU / mL.

5. The whitening and freckle-removing essence containing agarwood according to claim 2, characterized in that, The fermentation is carried out at a temperature of 30~37℃ and a stirring speed of 80~120r / min for 2~3 days.

6. The whitening and freckle-removing essence containing agarwood according to claim 1, characterized in that, The preparation method of the *Syzygium serratum* leaf extract is as follows: *Syzygium serratum* leaves are pulverized to obtain *Syzygium serratum* leaf powder. The *Syzygium serratum* leaf powder is soaked in ethanol solution A, and then subjected to ultrasonic extraction once. After filtration, filtrate and residue are obtained. The residue is subjected to ultrasonic extraction a second time with ethanol solution B, filtered, all filtrates are combined, the filtrate is concentrated and dried to obtain *Syzygium serratum* leaf extract.

7. The whitening and freckle-removing essence containing agarwood according to claim 6, characterized in that, The ratio of the leaves of the mountain jasmine to the ethanol solution in g / mL is 1:10~15; the soaking temperature is 40~50℃ and the soaking time is 2~3h; the ratio of the filter residue to the ethanol solution B in g / mL is 1:6~8.

8. The whitening and freckle-removing essence containing agarwood according to claim 6, characterized in that, The temperature of the first ultrasonic extraction is 50~60℃, the power is 200~300W, and the time is 60~90min; the temperature of the second ultrasonic extraction is 50~60℃, the power is 400~450W, and the time is 30~40min.

9. The whitening and freckle-removing essence containing agarwood according to claim 1, characterized in that, The emulsifier includes one or more of polyglycerol-4 oleate, cetearyl alcohol, and cetearyl glucoside; the thickener includes one or more of xanthan gum, carrageenan, carbomer, carboxymethyl cellulose, and hydroxypropyl cellulose; and the preservative includes one or more of phenoxyethanol, ethylhexylglycerin, methylparaben, and p-hydroxyacetophenone.

10. The preparation method of the whitening and freckle-removing essence containing agarwood according to claim 1, characterized in that, Includes the following steps: (1) Add panthenol, sodium hyaluronate, betaine, emulsifier, and thickener to water and stir to obtain a mixed solution; (2) Add agarwood fermentation extract, mountain jasmine leaf extract, white angelica root extract, tea jasmine extract, ectoine and preservative to the mixed solution and stir well to obtain the target whitening and spot-removing essence.

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