A bacteriostatic composition and its use in lotions

Abstract

The present application belongs to the technical field of bacteriostatic washing and caring products, and particularly relates to a bacteriostatic composition and application thereof in lotion. The bacteriostatic composition disclosed by the present application comprises a composite plant extract and a Rosa rugosa extract, wherein the composite plant extract is prepared by extracting and processing Smilax china L. powder, Ferula sinkiangensis K. M. Sheh. powder and Angelica dahurica Benth. powder through a specific process. The bacteriostatic composition of the present application has a synergistic effect among the components, a broad-spectrum bacteriostatic effect, excellent anti-parasitic activity, and is mild and non-irritating. In addition, the preparation method of the bacteriostatic composition is simple to operate, suitable for industrial production, and has good application value in the preparation of female private washing and caring products.

Description

Technical Field

[0001] This invention belongs to the technical field of antibacterial washing and care products, specifically relating to an antibacterial composition and its application in washing liquid. Background Technology

[0002] The female genitalia is a complex and delicate micro-ecosystem. Under normal physiological conditions, lactobacilli are the dominant flora. They produce lactic acid by breaking down glycogen in epithelial cells, maintaining an acidic environment with a vaginal pH of 3.8-4.5, thereby inhibiting the growth and reproduction of pathogenic bacteria and forming a natural defense barrier for the genitalia. However, modern women are easily affected by factors such as high work pressure, irregular work and rest, frequent use of antibiotics, and improper cleaning, which can easily lead to an imbalance in the genital micro-ecosystem. Pathogenic bacteria such as Gardnerella vaginalis, Streptococcus, Escherichia coli, Neisseria gonorrhoeae, and Candida albicans proliferate in large numbers. At the same time, they may also be attacked by parasites such as Trichomonas vaginalis, pinworms, and pubic lice, causing various gynecological diseases such as vaginitis and vulvitis, manifested as discomfort such as itching, redness, abnormal discharge, and odor, seriously affecting women's quality of life.

[0003] Currently, feminine hygiene products on the market are mainly divided into two categories: chemical antibacterial and plant-derived. Chemical antibacterial products often contain broad-spectrum chemical bactericides such as chlorhexidine, benzalkonium bromide, and triclosan. While these can quickly kill pathogens, they lack selectivity and can simultaneously disrupt the normal lactobacillus flora in the vagina, leading to a decline in the vagina's self-cleaning ability. Long-term use can easily cause dysbiosis, exacerbate inflammation, and may even lead to drug resistance in pathogens. Plant-derived products, while relatively milder, generally suffer from narrow antibacterial spectrum, low extraction rates of active ingredients, and unstable efficacy. Furthermore, most of these products lack antiparasitic activity and cannot comprehensively address vaginal infections. Therefore, developing a natural antibacterial composition with a broad antibacterial spectrum, antiparasitic effects, gentle and non-irritating properties, and the ability to maintain the vaginal microecological balance has significant practical importance and market value. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide an antibacterial composition and its application in washing solutions.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides an antibacterial composition comprising a compound plant extract and a wild rose extract; the compound plant extract is obtained by extraction from a compound plant powder, the compound plant powder comprising fig powder, asafoetida root powder, and angelica dahurica powder.

[0006] Preferably, the compound plant powder comprises fig powder, asafoetida root powder, and angelica dahurica powder in a mass ratio of 1:(0.5-1.3):(2-5).

[0007] Preferably, the preparation method of the compound plant extract includes the following steps: S1: Mix the compound plant powder and solvent at a material-to-liquid ratio of 1:(25-35) g / mL to obtain a mixed solution; S2: Heat the mixed solution to 40-60℃, extract 2-3 times, each time for 0.5-1h, then filter, and combine the filtrates to obtain a crude extract; S3: Concentrate the crude extract by rotary evaporation to 1 / 5 of the initial crude extract volume, then add deionized water to 1 / 2 of the initial crude extract volume, and rotary evaporate again to 1 / 5 of the initial crude extract volume. Repeat the above operation of adding deionized water and rotary evaporation concentration 3 times, and then freeze-dry to obtain the compound plant extract powder; wherein the solvent is a compound solvent of ethyl acetate and n-hexane, wherein the molar ratio of ethyl acetate to n-hexane is 1:(2-4).

[0008] Preferably, the mass ratio of the compound plant extract to the wild rose extract is 1:(0.2-0.5).

[0009] In a second aspect, the present invention provides an antibacterial wash solution comprising the antibacterial composition described in any one of the above claims.

[0010] Preferably, the antibacterial wash also includes fragrance, solvent and preservative.

[0011] Preferably, the solvent is deionized water and the preservative is phenoxyethanol.

[0012] Thirdly, the present invention provides the use of the antibacterial composition described in any one of the above claims in the preparation of a personal care product with antibacterial effects.

[0013] Preferably, the washing and care product is a feminine hygiene product.

[0014] Preferably, the antibacterial effect includes inhibition of at least one of Gardnerella vaginalis, Streptococcus, Escherichia coli, Neisseria gonorrhoeae, and Candida albicans.

[0015] Fourthly, the present invention provides the use of the antibacterial composition described in any one of the above claims in the preparation of a personal care product with antiparasitic efficacy.

[0016] Preferably, the parasite includes Trichomonas vaginalis.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: The active ingredients of the antibacterial composition provided by this invention are all derived from natural plants. Through scientific compounding, the synergistic effect between the components is achieved, which has the dual effects of broad-spectrum antibacterial and excellent antiparasitic effects. At the same time, it is mild and non-irritating. In vitro tests show that it has no significant inhibitory effect on vaginal lactobacilli, and is expected to effectively maintain the microecological balance of women's private parts.

[0018] The compound plant extract in this invention is prepared from fig powder, asafoetida root powder, and angelica dahurica powder. Fig extract is rich in flavonoids, terpenoids, and organic acids, exhibiting broad-spectrum antibacterial activity. It can inhibit the growth and reproduction of various pathogenic bacteria and also has anti-inflammatory and swelling-reducing effects, alleviating inflammation of the vulvar mucosa and promoting the repair of damaged mucosa. The core active ingredient in asafoetida root extract, ferulic acid, has significant antibacterial, antioxidant, and immunomodulatory effects. It can disrupt the cell membrane structure of pathogenic bacteria, inhibit biofilm formation, thereby enhancing the antibacterial effect. It can also scavenge free radicals and reduce oxidative stress damage to vulvar tissues. Angelica dahurica extract contains abundant coumarins and volatile oils, which not only have good inhibitory effects on various fungi and bacteria but also dispel wind and dampness, relieve itching and pain, and quickly alleviate discomfort symptoms such as vulvar itching and redness.

[0019] Wild rose extract is rich in polyphenols, tannins, and flavonoids, exhibiting strong inhibitory effects against pathogenic bacteria such as Gardnerella vaginalis and Candida albicans. It also shows significant inhibitory effects against Trichomonas vaginalis. Its astringent components can tighten the vaginal mucosa, enhance its barrier function, and improve the vagina's natural defenses. This invention combines compound plant extracts with wild rose extract in a specific ratio. The components work synergistically to significantly broaden the antibacterial spectrum, exhibiting strong inhibitory effects against common vaginal pathogens such as Gardnerella vaginalis, Streptococcus, Escherichia coli, Neisseria gonorrhoeae, and Candida albicans. Furthermore, the composition possesses excellent anti-Trichomonas vaginalis activity, effectively addressing common bacterial, fungal, and Trichomonas vaginalis infections in the female genital area.

[0020] In the preparation process of the compound plant extract, this invention uses a composite solvent composed of ethyl acetate and n-hexane for extraction. Utilizing the polarity gradient of the two solvents, it can more comprehensively extract weakly polar to non-polar lipid-soluble active ingredients from plants, increasing the content of active substances in the extract. Simultaneously, most of the organic solvent is removed through multiple rotary evaporation concentrations combined with deionized water replacement, followed by freeze-drying to obtain the extract powder. This process minimizes residual organic solvents while ensuring uniform dispersion of active ingredients and guaranteeing product stability. The entire preparation process is simple to operate, operates under mild conditions, requires no complex equipment or harsh reaction conditions, and is suitable for large-scale industrial production.

[0021] The antibacterial composition prepared by this invention does not contain chemically synthesized bactericides, is non-irritating to the vaginal mucosa, and in vitro tests show that it has no significant inhibitory effect on dominant vaginal lactobacilli. It helps maintain the microecological balance of the private parts and has broad application prospects and market value. Detailed Implementation

[0022] To further illustrate the technical means and effects adopted by the present invention to achieve its intended purpose, the following detailed description, in conjunction with embodiments, provides a detailed explanation of the specific implementation methods, structures, features, and effects of the present invention. Experimental methods in the following embodiments, unless otherwise specified, are generally performed under conventional conditions or according to the manufacturer's recommendations. Unless otherwise specified, all materials and reagents used are commercially available.

[0023] Some of the raw materials and their sources are as follows: Ficus pumila powder, Ferula assa-foetida root powder, and Angelica dahurica powder are all made by washing, removing impurities, drying, and then grinding the Chinese medicinal materials into powder and passing them through an 80-mesh sieve.

[0024] The wild rose extract is the wild rose root extract prepared in Example 1 of patent CN202411875011.4; Its preparation method is as follows: T1. Dry the wild rose root to constant weight, then pulverize the wild rose root using a micro-airflow pulverizer to obtain A1, which is then sealed and stored for later use. T2. A1 was extracted by reflux using water-saturated ethyl acetate, and the extract A2 and residue A3 were obtained by filtration. The reflux extraction temperature was 50℃, the reflux extraction time was 1.5h, and the mass ratio of water-saturated ethyl acetate to A1 was 40:1. T3. Concentrate and freeze-dry A2 to obtain A4, then seal, protect from light, and freeze for later use. T4. After drying A3 to constant weight at 82℃, pulverize it to obtain A5. Add twice the mass of deionized water of A5 and stir evenly to obtain a mixture. Use cellulase, ligninase and pectinase to enzymatically hydrolyze the mixture. After enzymatic hydrolysis for 2 hours, filter to obtain filtrate A6. The mass ratio of cellulase, ligninase and pectinase is 1:2:2, and the total mass of cellulase, ligninase and pectinase is 9% of the mass of A5. T5. Ferment A6 using Rhodotorula glutinis, filter, and obtain fermentation product A7. The fermentation time is 60 h, the initial fermentation pH is 5.8, the fermentation temperature is 30 ℃, and the amount of Rhodotorula glutinis added is 10% of the mass of A6. T6. The fermentation product A7 is ultrasonically crushed for 10 minutes, filtered and sterilized to obtain A8. T7, A4, and A8 were mixed to obtain wild rose root extract.

[0025] The preparation method of witch hazel extract is as follows: Take 100 g of dried witch hazel (Hamamelis virginiana L.) leaves, add 10 times the amount of purified water, reflux at 80℃ twice, 1 h each time, combine the filtrates, concentrate under reduced pressure to a relative density of 1.15, freeze dry to obtain witch hazel extract.

[0026] Ethyl acetate and n-hexane were of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. The components and process parameters of the antibacterial compositions in the embodiments and comparative examples of the present invention are shown in Table 1 below. The total mass of the antibacterial washing solution in Examples 1-3 and Comparative Examples ①-⑨ is the same. The amount of antibacterial composition added to the washing solution is 5 wt%, the amount of fragrance added is 0.1 wt%, the amount of phenoxyethanol added is 0.5 wt%, and the balance is deionized water.

[0027] Table 1. Components and key process parameters of the antibacterial compositions in the examples and comparative examples. Example 1 1:0.9:3.5 1:0.35 1:3 1:30 50 2-0.75 have Example 2 1:0.5:2 1:0.2 1:2 1:25 40 3-0.5 have Example 3 1:1.3:5 1:0.5 1:4 1:35 60 2-1 have Comparative Example ① - 0:1 - - - - - Comparative Example ② 1:0.9:3.5 1:0 1:3 1:30 50 2-0.75 have Comparative Example ③ 0:0.9:4.5 1:0.35 1:3 1:30 50 2-0.75 have Comparative Example 4 1:0:4.4 1:0.35 1:3 1:30 50 2-0.75 have Comparative Example ⑤ 1:0.9:0 1:0.35 1:3 1:30 50 2-0.75 have Comparative Example 6 1:0.9:3.5 1:0.35 (Witch Hazel) 1:3 1:30 50 2-0.75 have Comparative Example ⑦ 1:0.9:3.5 1:0.35 1:0 1:30 50 2-0.75 have Comparative Example ⑧ 1:0.9:3.5 1:0.35 0:1 1:30 50 2-0.75 have Comparative Example 9 1:0.9:3.5 1:0.1 1:3 1:30 50 2-0.75 have Note: Comparative Example ① contains only wild rose extract and no compound plant extracts; Comparative Example ② contains only compound plant extracts and no wild rose extract; in Comparative Example ⑥, wild rose extract was replaced with an equal mass of witch hazel extract; in Comparative Example ⑦, the extraction solvent was only ethyl acetate; in Comparative Example ⑧, the extraction solvent was only n-hexane.

[0028] Example 1: Preparation of antibacterial composition and antibacterial wash solution Preparation of compound plant extract: S1: Weigh out Ficus pumila powder, Ferula assa-foetida root powder and Angelica dahurica powder in a mass ratio of 1:0.9:3.5, mix them evenly to obtain compound plant powder, and mix the compound plant powder with ethyl acetate-n-hexane compound solvent (molar ratio 1:3) at a material-liquid ratio of 1:30 g / mL to obtain a mixed solution; S2: Heat the mixed solution to 50℃ and extract twice, each time for 0.75 h. After extraction, filter with qualitative filter paper and combine the two filtrates to obtain crude extract; S3: Place the crude extract in a rotary evaporator and concentrate it to 1 / 5 of the initial crude extract volume at 50℃ and 0.06 MPa pressure. Then add deionized water to 1 / 2 of the initial crude extract volume, and rotary evaporate again to 1 / 5 of the initial crude extract volume. Repeat the above operation of adding deionized water and rotary evaporation concentration 3 times. Finally, freeze-dry the concentrate to obtain compound plant extract powder.

[0029] Preparation of antibacterial wash solution: Weigh the above-mentioned compound plant extract powder and wild rose extract at a mass ratio of 1:0.35, mix them evenly to obtain an antibacterial composition; take 5 parts of the antibacterial composition, add 0.1 parts of fragrance, 0.5 parts of phenoxyethanol and 94.4 parts of deionized water, stir at room temperature for 30 minutes until completely dissolved to obtain the antibacterial wash solution.

[0030] Example 2: Preparation of antibacterial composition and antibacterial wash solution Preparation of compound plant extract: S1: Weigh out Ficus pumila powder, Ferula assa-foetida root powder and Angelica dahurica powder according to a mass ratio of 1:0.5:2, mix them evenly to obtain compound plant powder, and mix the compound plant powder with ethyl acetate-n-hexane compound solvent (molar ratio 1:2) at a material-liquid ratio of 1:25 g / mL to obtain a mixed solution; S2: Heat the mixed solution to 40℃ and extract 3 times, 0.5 h each time. After extraction, filter with qualitative filter paper and combine the three filtrates to obtain crude extract; S3: Place the crude extract in a rotary evaporator and concentrate it to 1 / 5 of the initial crude extract volume at 50℃ and 0.06 MPa pressure. Then add deionized water to 1 / 2 of the initial crude extract volume, and rotary evaporate again to 1 / 5 of the initial crude extract volume. Repeat the above operation of adding deionized water and rotary evaporation concentration 3 times. Finally, freeze-dry the concentrate to obtain compound plant extract powder.

[0031] Preparation of antibacterial wash solution: Weigh the above-mentioned compound plant extract powder and wild rose extract at a mass ratio of 1:0.2, mix them evenly to obtain an antibacterial composition; take 5 parts of the antibacterial composition, add 0.1 parts of fragrance, 0.5 parts of phenoxyethanol and 94.4 parts of deionized water, stir at room temperature for 30 minutes until completely dissolved to obtain the antibacterial wash solution.

[0032] Example 3: Preparation of antibacterial composition and antibacterial wash solution Preparation of compound plant extract: S1: Weigh out Ficus pumila powder, Ferula assa-foetida root powder and Angelica dahurica powder in a mass ratio of 1:1.3:5, mix them evenly to obtain compound plant powder, and mix the compound plant powder with ethyl acetate-n-hexane compound solvent (molar ratio 1:4) at a material-liquid ratio of 1:35 g / mL to obtain a mixed solution; S2: Heat the mixed solution to 60℃ and extract twice, 1 hour each time. After extraction, filter with qualitative filter paper and combine the two filtrates to obtain crude extract; S3: Place the crude extract in a rotary evaporator and concentrate it to 1 / 5 of the initial crude extract volume at 50℃ and 0.06 MPa pressure. Then add deionized water to 1 / 2 of the initial crude extract volume, and rotary evaporate again to 1 / 5 of the initial crude extract volume. Repeat the above operation of adding deionized water and rotary evaporation concentration 3 times. Finally, freeze-dry the concentrate to obtain compound plant extract powder.

[0033] Preparation of antibacterial wash solution: Weigh the above-mentioned compound plant extract powder and wild rose extract at a mass ratio of 1:0.5, mix them evenly to obtain an antibacterial composition; take 5 parts of the antibacterial composition, add 0.1 parts of fragrance, 0.5 parts of phenoxyethanol and 94.4 parts of deionized water, stir at room temperature for 30 minutes until completely dissolved to obtain the antibacterial wash solution.

[0034] The preparation methods of the antibacterial wash solutions described in Comparative Examples ①-⑨ are the same as those in Example 1, except for the differences noted in Table 1 above. In Comparative Example ①, there is no need to prepare the compound plant extract; the wild rose extract is directly weighed and prepared into a wash solution in the same proportion.

[0035] Performance testing Test Example 1 Before the experiment, Gardnerella vaginalis (BMZ115358), Streptococcus (BMZ146133), Escherichia coli (B81038), Neisseria gonorrhoeae (BMZ135835), and Candida albicans (B81066) were streaked onto their respective agar slants; all the above bacterial strains were from Mingzhou Biotechnology. Gardnerella vaginalis, Streptococcus, and Neisseria gonorrhoeae: Incubate at 36.0℃ and 5% CO2 for 24 hours; Escherichia coli: cultured at 36.0℃ under aerobic conditions for 24 hours; Candida albicans: cultured at 28.0℃ for 48 h; Wash the cultured test bacteria off with the corresponding commercially available liquid culture medium for the appropriate bacterial strain, and adjust the bacterial suspension concentration to 1×10⁻⁶. 8 CFU / mL, for later use; Gardnerella vaginalis was cultured on a brain and heart broth containing 5% fetal bovine serum, Neisseria gonorrhoeae on a chocolate broth, Escherichia coli on an LB broth, Candida albicans on a Sabouraud dextrose broth, and Streptococcus on a brain and heart broth.

[0036] Detection method: The antibacterial rate was determined using the 96-well plate microdilution method, with three replicates per group, and a blank control group and a negative control group were also included. Group settings: Blank control group: 180 μL liquid culture medium + 20 μL sterile PBS; Negative control group: 160 μL liquid culture medium + 20 μL bacterial suspension + 20 μL sterile PBS; Experimental group: 160 μL liquid culture medium + 20 μL bacterial suspension + 20 μL sample solution (example / comparative wash solution); Culture conditions: Place the 96-well plate under suitable conditions for each bacterial strain and culture for 24 hours (bacteria) or 48 hours (Candida albicans). OD value determination: The absorbance (OD) of each well was measured at a wavelength of 600 nm using a microplate reader. 600 The average OD value of each group was calculated after deducting the OD value of the blank control group.

[0037] Antibacterial rate (%) = (OD negative control - OD test group) / OD negative control × 100% Evaluation criteria: 50% ≤ antibacterial rate < 90%, the product has antibacterial effect; antibacterial rate ≥ 90%, the product has strong antibacterial effect.

[0038] The experimental results are shown in Table 2 below.

[0039] Table 2. Inhibition results of the examples and comparative examples against different harmful bacteria. Example 1 83.4 90.9 93.8 90.2 87.9 Example 2 81.1 86.8 91.5 85.9 90.6 Example 3 79.6 89.8 88.5 91.8 86.7 Comparative Example ① 28.4 39.1 61.2 40.6 58.4 Comparative Example ② 41.6 57.9 64.3 47.2 73.5 Comparative Example ③ 45.2 48.8 68.5 36.3 51.8 Comparative Example 4 60.3 32.5 52.1 30.1 42.6 Comparative Example ⑤ 62.8 41.3 65.4 48.9 50.5 Comparative Example 6 55.6 58.2 66.4 54.3 60.1 Comparative Example ⑦ 68.5 62.4 79.2 75.6 72.8 Comparative Example ⑧ 52.3 71.5 48.6 50.2 65.4 Comparative Example 9 67.8 84.6 89.3 82.9 84.2 Test results showed that Examples 1-3 all exhibited good antibacterial effects against five common pathogens, with Example 1 showing the best overall antibacterial ability. Example 2 showed specific advantages against Candida albicans, and Example 3 showed specific advantages against Neisseria gonorrhoeae. The overall antibacterial effects of Comparative Examples ①-⑨ were significantly lower than the overall level of the Example groups, indicating that the composition of the compound plant extract and Rosa rugosa extract, the ratio of each raw material, and the preparation process of the compound plant extract all significantly affect the antibacterial effect of the composition. Specifically, Comparative Examples ①-⑤ lacked the compound plant extract, Rosa rugosa extract, and the Ficus pumila powder, Ferula assa-foetida root powder, and Angelica dahurica powder in the compound plant powder, respectively, resulting in a significant decrease in antibacterial effect. This indicates that there is a significant synergistic effect among the components, and none can be omitted. In Comparative Example ⑥, replacing Rosa rugosa extract with witch hazel extract significantly worsened the antibacterial effect, indicating that Rosa rugosa extract plays an irreplaceable role in this composition. Comparative Examples ⑦ and ⑧ used only ethyl acetate or n-hexane as solvents for extraction, which could not fully extract all kinds of active ingredients in the plants, resulting in a decrease in antibacterial effect. Comparative Example ⑨ changed the mass ratio of compound plant extract to wild rose extract, which exceeded the scope of the present invention, making it difficult to achieve the best synergistic effect, and the antibacterial effect also decreased.

[0040] Test Example 2 Trichomonas vaginalis test: Trichomonas vaginalis in the logarithmic growth phase was collected and the concentration of the parasites was adjusted to 1×10⁻⁶ using liver infusion culture medium. 5 / mL. Take 0.5mL of the above insect suspension and mix it with 4.5mL of the sample solution (Examples 1-3 and Comparative Examples ①-⑨). After culturing under anaerobic conditions at 37℃ for 48h, count the number of surviving insects using a hemocytometer and calculate the insect inhibition rate.

[0041] The blank control group used a wash base (0.1 parts fragrance, 0.5 parts phenoxyethanol and 99.4 parts deionized water) without the antibacterial composition instead of the sample solution.

[0042] The inhibition rate is calculated using the formula: Y = (CD) / C × 100% In the formula: Y is the inhibition rate, %; C is the number of surviving parasites in the blank control group; D is the number of surviving parasites in the experimental group.

[0043] Evaluation criteria: Inhibition rate ≥90% indicates strong inhibitory activity, 50%-90% indicates inhibitory activity, and <50% indicates no significant inhibitory activity.

[0044] The experimental results are shown in Table 3 below.

[0045] Table 3. Inhibition rates of the parasites in the examples and comparative examples. Example 1 90.2 Example 2 86.1 Example 3 88.9 Comparative Example ① 49.2 Comparative Example ② 52.6 Comparative Example ③ 40.5 Comparative Example 4 33.7 Comparative Example ⑤ 42.8 Comparative Example 6 62.1 Comparative Example ⑦ 66.8 Comparative Example ⑧ 64.7 Comparative Example 9 81.5 Test results showed that Examples 1-3 had good inhibitory effects on Trichomonas vaginalis, with Example 1 exhibiting the best overall antiparasitic effect. The antiparasitic activities of Comparative Examples ①-⑨ were significantly lower than those of Example 1, further verifying the synergistic effect of the components in the composition and the rationality of the preparation process. The rational combination of compound plant extracts and Rosa rugosa extract not only achieved broad-spectrum antibacterial activity but also endowed the composition with excellent antiparasitic activity, effectively addressing common bacterial, fungal, and Trichomonas vaginalis infections in the female private parts.

[0046] Test Example 3 Vaginal lactobacillus effect test Preparation of bacterial suspension: Lactobacillus curvature and Lactobacillus acidophilus were inoculated onto MRS agar slants and cultured at 37°C and 5% CO2 for 48 h. The culture was then washed down with MRS liquid medium to obtain a viable count of 1 × 10⁻⁶. 8 CFU / mL bacterial suspension.

[0047] Group settings: Blank control group: 180 μL MRS liquid culture medium + 20 μL sterile PBS; Negative control group: 160 μL MRS liquid culture medium + 20 μL bacterial suspension + 20 μL sterile PBS; Experimental group: 160 μL MRS liquid culture medium + 20 μL bacterial suspension + 20 μL sample solution (Examples 1-3); Detection method: Culture conditions: Place the 96-well plate under suitable conditions for each bacterial strain and incubate for 24 hours; OD value determination: The absorbance (OD) of each well was measured at a wavelength of 600 nm using a microplate reader. 600 The average OD value of each group was calculated after deducting the OD value of the blank control group.

[0048] Antibacterial rate (%) = (OD negative control - OD test group) / OD negative control × 100% Evaluation criteria: Antibacterial rate <20%, considered to have no significant impact on beneficial bacteria.

[0049] The experimental results are shown in Table 4.

[0050] Table 4. Inhibitory effect of the washing solution on lactobacilli Example 1 12.7 12.1 Example 2 10.9 9.8 Example 3 14.7 13.2 The test results show that the antibacterial composition of the present invention has no significant inhibitory effect on the dominant lactobacilli in the vagina and will not disrupt the normal microecological balance of the private parts.

[0051] Test Example 4 Laboratory animals: female New Zealand rabbits, weighing 2.0-2.5 kg.

[0052] Before the experiment, the rabbits were examined to ensure there was no discharge, congestion, edema, or other damage at the vaginal opening.

[0053] They are fed with ordinary feed under constant temperature conditions free of special pathogens, at a temperature of 24-28℃ and a relative humidity of 50%-60%, and are housed in individual cages.

[0054] Detection Method: Experimental animals were randomly divided into a treatment group (Examples 1-3) and a blank control group. The test solution for the treatment group was the antibacterial wash prepared in Examples 1-3, while the test solution for the blank control group was 0.9% physiological saline. Three animals were used in each group. An 8cm blunt-tipped flexible tube was connected to a 2ml syringe. Both the syringe and the tube were filled with the test solution and prepared for use. The animals were fixed supine, exposing the perineum and vaginal opening. The tube, moistened with the test solution, was gently inserted 5cm into the vagina, and 2ml of the test solution was slowly injected using the syringe. The tube was then withdrawn, completing the treatment. Treatment was repeated every 24 hours for 5 consecutive days. The control group animals were treated with physiological saline in the same way. 24 hours after the last treatment, the animals were euthanized using the air embolism method. The intact vagina was removed by laparotomy, longitudinally incised, and visually inspected for signs of congestion and edema. The vagina was then fixed in 10% formalin solution for 24 hours. Tissue sections were prepared from three locations: both ends and the center of the vagina. After HE staining, histopathological examination was performed. The vaginal mucosal stimulation response was scored according to the standard (GB / T16886.23-2023 / ISO10993-23:2021 Biological evaluation of medical devices - Part 23: Stimulation tests - D.6 Vaginal stimulation test). The stimulation response scores of the three animals in each group were added together and then divided by the total number of observations (number of animals × 3) to obtain the average score of the vaginal mucosal stimulation response in each group. The stimulation index was obtained by subtracting the average score of the control group from the average score of the poisoned group, and the stimulation intensity was graded according to the standard.

[0055] Test results: No abnormalities such as congestion, edema, or erosion were observed in the vaginal mucosa of animals in both the blank control group and the poisoned group. Histopathological examination showed that the epithelial tissue was intact, without leukocyte infiltration, vascular congestion, or edema. The irritation index was less than 1, indicating that the antibacterial composition prepared in this invention is mild and non-irritating, and has a basis for long-term safety.

[0056] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An antibacterial composition, characterized in that, The composition includes a compound plant extract and a wild rose extract; the compound plant extract is obtained by extracting a compound plant powder, which includes fig powder, asafoetida root powder, and angelica dahurica powder. The wild rose extract is a wild rose root extract, and the preparation method of the wild rose root extract includes the following steps: T1. Dry the wild rose root to constant weight, then pulverize the wild rose root using a micro-airflow pulverizer to obtain A1, which is then sealed and stored for later use. T2. A1 was extracted by reflux using water-saturated ethyl acetate, and the extract A2 and residue A3 were obtained by filtration. The reflux extraction temperature was 50℃, the reflux extraction time was 1.5h, and the mass ratio of water-saturated ethyl acetate to A1 was 40:

1. T3. Concentrate and freeze-dry A2 to obtain A4, then seal, protect from light, and freeze for later use. T4. After drying A3 to constant weight at 82℃, pulverize it to obtain A5. Add twice the mass of deionized water of A5 and stir evenly to obtain a mixture. Use cellulase, ligninase and pectinase to enzymatically hydrolyze the mixture. After enzymatic hydrolysis for 2 hours, filter to obtain filtrate A6. The mass ratio of cellulase, ligninase and pectinase is 1:2:2, and the total mass of cellulase, ligninase and pectinase is 9% of the mass of A5. T5. Ferment A6 using Rhodotorula glutinis, filter, and obtain fermentation product A7. The fermentation time is 60 h, the initial fermentation pH is 5.8, the fermentation temperature is 30 ℃, and the amount of Rhodotorula glutinis added is 10% of the mass of A6. T6. The fermentation product A7 is ultrasonically crushed for 10 minutes, filtered and sterilized to obtain A8. T7, mixed with A4 and A8, yielded wild rose root extract; The compound plant powder includes fig powder, asafoetida root powder, and angelica powder in a mass ratio of 1:(0.5-1.3):(2-5); The preparation method of the compound plant extract includes the following steps: S1: Mix the compound plant powder and solvent at a material-to-liquid ratio of 1:(25-35)g / mL to obtain a uniform mixture; S2: Heat the mixture to 40-60℃, extract 2-3 times, each time for 0.5-1 hour, then filter and combine the filtrates to obtain the crude extract; S3: The crude extract is concentrated by rotary evaporation to 1 / 5 of the initial crude extract volume, then deionized water is added to 1 / 2 of the initial crude extract volume, and rotary evaporation is repeated to 1 / 5 of the initial crude extract volume. The above operation of adding deionized water and rotary evaporation is repeated 3 times, and then freeze-dried to obtain compound plant extract powder. The solvent is a composite solvent of ethyl acetate and n-hexane, wherein the molar ratio of ethyl acetate to n-hexane is 1:(2-4). The mass ratio of the compound plant extract to the wild rose extract is 1:(0.2-0.5).

2. The use of the composition as described in claim 1 in the preparation of a feminine hygiene product with antibacterial properties.

3. The application as described in claim 2, characterized in that, The antibacterial effect includes inhibition of at least one of Gardnerella vaginalis, Streptococcus, Escherichia coli, Neisseria gonorrhoeae, and Candida albicans.

4. The use of the composition of claim 1 in the preparation of a feminine wash product with antiparasitic efficacy; wherein the parasite is Trichomonas vaginalis.

5. An antibacterial wash solution, characterized in that, Includes the antibacterial composition of claim 1.