Junctional facial mask based on nine kinds of constitution typing and biological fermentation technology and preparation method thereof

Abstract

The present application relates to a nine-constitution type and bio-fermentation technology-based facial mask of monarch, minister, assistant and messenger and a preparation method thereof, and belongs to the field of cosmetics and biological cross technology. The present application sets up a facial mask formula for each of the nine kinds of Chinese medical constitution, namely, normal constitution, qi deficiency constitution, yang deficiency constitution, yin deficiency constitution, phlegm-damp constitution, damp-heat constitution, blood stasis constitution, qi depression constitution and special constitution. Each formula contains four levels, namely, monarch, minister, assistant and messenger. The facial mask product is prepared by compounding the mask substrate with the traditional Chinese medicine raw materials after probiotic fermentation treatment. A constitution identification system based on five-dimensional evaluation is used to achieve accurate recommendation. The present application organically integrates Chinese medical constitution typing, monarch-minister-assistant-messenger combination and bio-fermentation technology, realizes personalized skin care of "constitution identification and mask application", improves the content of active ingredients and skin absorption rate through fermentation treatment, and has the characteristics of safety, mildness and remarkable efficacy.

Description

Technical Field

[0001] This invention relates to the field of cosmetics and biotechnology, specifically to a "principal-assistant-adjuvant" facial mask based on nine body constitution classifications and bio-fermentation technology, and its preparation method. Background Technology

[0002] Traditional Chinese medicine (TCM) facial masks are skincare products made with extracts or powders of Chinese medicinal herbs as the main active ingredients, combined with a mask base. In recent years, with the popularization of the TCM beauty concept of "internal conditioning and external nourishment" and the increasing demand from consumers for skincare products that are natural, safe, and effective, the TCM facial mask market has continued to expand. In existing technologies, some TCM facial mask products have attempted to incorporate the TCM theory of principal, assistant, and adjuvant herbs into their formulation design—for example, masks using Ganoderma lucidum as the principal herb, Codonopsis pilosula as the assistant herb, and lotus as the adjuvant herb; masks for improving skin elasticity using Paeonia lactiflora as the principal herb, Lycium barbarum as the assistant herb, and Carthamus tinctorius as the adjuvant herb; and anti-aging masks using Codonopsis pilosula and Astragalus membranaceus as the principal herb and Poria cocos as the assistant herb—these products utilize the principles of principal, assistant, and adjuvant herbs to guide their formulation to some extent. Furthermore, bio-fermentation technology has also been explored in the field of TCM facial masks, such as using enzyme bio-fermentation technology to process Chinese medicinal herbs to prepare facial mask products, utilizing microbial fermentation to improve the utilization rate of active ingredients and skin absorption rate. However, a comprehensive analysis of existing technologies reveals the following main problems: First, although the theory of principal, assistant, adjuvant, and guide formulas is reflected in some products, existing products are all fixed formulas designed for single effects (such as whitening, wrinkle reduction, and anti-aging), failing to systematically integrate the principal, assistant, adjuvant, and guide formulas with the nine TCM constitution classifications, and thus unable to make targeted adjustments to the formulas based on different user constitution types. Second, the application of bio-fermentation technology in TCM facial masks also fails to integrate with the constitution classification theory, and the application of fermentation technology is limited to general formulas. Third, some scholars have proposed the academic idea of ​​developing skincare products based on the nine constitution classifications and have published related monographs, but this idea remains at the theoretical level and has not yet formed a specific series of facial mask products for the nine constitutions. In conclusion, the current field of TCM facial masks lacks a technical solution that systematically integrates the nine constitution classifications, the principal, assistant, adjuvant, and guide formulas, and bio-fermentation technology.

[0003] Traditional Chinese medicine (TCM) constitution theory classifies human constitution into nine basic types: balanced, qi deficiency, yang deficiency, yin deficiency, phlegm-dampness, damp-heat, blood stasis, qi stagnation, and special constitution. In 2025, the State Administration for Market Regulation (National Standardization Management Committee) approved and released the recommended national standard "Classification and Determination of TCM Constitutions" (GB / T 46939-2025), which stipulates the basic characteristics of the nine constitution types and their determination methods. Significant differences exist in skin conditions among people with different constitution types: those with qi deficiency often have a sallow complexion and loose skin; those with yang deficiency often have a pale complexion and cold skin; those with yin deficiency often have dry skin and fine lines; those with phlegm-dampness and damp-heat often have oily skin and acne; those with blood stasis often have a dull complexion and pigmentation; those with qi stagnation often have uneven skin tone and melasma; and those with special constitution often have sensitive skin and allergies. This inherent connection between constitution and skin problems provides a theoretical basis for personalized skincare based on "body type and skin treatment." However, currently available Chinese herbal medicine facial mask products are mainly general-purpose or targeting single effects. There is no complete product series covering all nine TCM constitution types, nor are there reports of a systematic technical solution organically combining the nine constitution types, the principles of monarch-minister-assistant-guide prescription, and bio-fermentation technology. Therefore, developing a series of facial mask products that can cover all nine TCM constitution types, follow the principles of monarch-minister-assistant-guide prescription, and be prepared using bio-fermentation technology, along with its preparation method, has significant market value and technological importance. Summary of the Invention

[0004] In order to solve the problems of the prior art, the present invention provides a monarch-minister-assistant-guide facial mask based on nine body constitution classifications and bio-fermentation technology and its preparation method.

[0005] To solve the above-mentioned technical problems, the present invention is achieved through the following technical solution: Firstly, a mask based on nine constitution types and bio-fermentation technology, wherein the mask contains nine mask formulas respectively set for nine TCM constitutions: balanced constitution, qi deficiency constitution, yang deficiency constitution, yin deficiency constitution, phlegm-dampness constitution, damp-heat constitution, blood stasis constitution, qi stagnation constitution and special constitution. Each of the aforementioned mask formulations comprises four levels of raw materials: principal ingredient, assistant ingredient, adjuvant ingredient, and guide ingredient. The principal ingredient plays a major role in addressing the core skin problems of the corresponding constitution and is used in the largest quantity. The assistant ingredient assists the principal ingredient in enhancing its efficacy or addresses secondary skin problems. The adjuvant ingredient adjusts the properties of the formulation or provides auxiliary effects. The guide ingredient guides the penetration of active ingredients and harmonizes the various ingredients. The traditional Chinese medicine ingredients in the mask formula are fermented with probiotics to obtain a concentrated fermentation liquid, which is then compounded with the mask matrix to form a mask preparation.

[0006] In one specific implementation of the first aspect, the correspondence between the nine body types and skin problems is as follows: The balanced constitution corresponds to normal skin that is moisturized and elastic; the Qi deficiency constitution corresponds to a sallow or pale complexion and loose skin; the Yang deficiency constitution corresponds to a pale complexion and cold skin; the Yin deficiency constitution corresponds to dry skin, fine lines, and flushed cheeks; the phlegm-dampness constitution corresponds to oily skin, large pores, and a tendency to develop acne; the damp-heat constitution corresponds to oily skin, acne, and itchy skin; the blood stasis constitution corresponds to a dull complexion, pigmentation, and age spots; the Qi stagnation constitution corresponds to a sallow complexion and a tendency to develop age spots; and the special constitution corresponds to sensitive skin, allergies, and telangiectasia.

[0007] In one specific embodiment of the first aspect, the principal ingredient in the nine body constitution mask formulas is selected from the following ingredients: balanced constitution is selected from yam, qi deficiency constitution is selected from ginseng, yang deficiency constitution is selected from cinnamon, yin deficiency constitution is selected from Solomon's seal, phlegm-dampness constitution is selected from coix seed, damp-heat constitution is selected from coix seed, blood stasis constitution is selected from salvia miltiorrhiza, qi stagnation constitution is selected from rose, and special constitution is selected from dried plum.

[0008] In one specific embodiment of the first aspect, the probiotic is selected from one or more of Saccharomyces cerevisiae, lactic acid bacteria, Bifidobacterium and Bacillus subtilis.

[0009] In one specific embodiment of the first aspect, the raw materials in the nine body type mask formulas are all cosmetic raw materials that meet the requirements of the "Cosmetic Safety Technical Specifications" and the "Catalogue of Used Cosmetic Raw Materials", or are substances listed in the national catalogue of food and medicine homology.

[0010] Secondly, a method for preparing a facial mask includes the following steps: (1) Raw material pretreatment: The Chinese medicinal materials in each mask formula are cleaned, washed, dried at low temperature and then ultra-finely pulverized to a particle size ≤20μm. They are weighed and mixed according to the ratio of principal, assistant and adjuvant herbs to obtain mixed medicinal powder. (2) Preparation of fermentation substrate: Add the mixed powder to purified water, add carbon source and nitrogen source, adjust pH to 5.5-6.5, sterilize, and obtain fermentation substrate; (3) Inoculation and fermentation: Cool the fermentation substrate to 28-37℃, inoculate with activated probiotics, and ferment at 28-37℃ for 24-72 hours to obtain the fermentation product; (4) Fermentation broth treatment: Remove the cell bodies from the fermentation product and concentrate it under low temperature and vacuum to obtain fermentation concentrate; (5) Preparation of facial mask formulation: The fermentation concentrate is combined with the facial mask matrix to prepare a facial mask formulation; (6) Filling and packaging.

[0011] In a specific embodiment of the second aspect, the ratio of the mixed medicinal powder to purified water in step (2) is 1:5-10, the carbon source is glucose or sucrose and the amount added is 1%-3%, the nitrogen source is yeast powder or peptone and the amount added is 0.5%-1%, and the sterilization conditions are sterilization at 121℃ for 15-20 minutes; the inoculation amount of probiotics in step (3) is 1%-5%; the temperature of the low-temperature vacuum concentration in step (4) is ≤60℃, and the relative density of the fermentation concentrate is 1.05-1.15.

[0012] In one specific embodiment of the second aspect, the mask preparation is a sheet mask, and step (5) specifically includes: Add the fermentation concentrate and moisturizer to deionized water, stir and heat to 70-80℃; add thickener, homogenize and stir for 10-15 minutes; cool to 40-45℃, add active ingredients, stir evenly; cool to 35℃, add preservative, adjust pH to 5.5-6.5 to obtain essence; soak the mask sheet in the essence for 4-8 hours, remove, fold, pack and seal.

[0013] In one specific embodiment of the second aspect, the mask preparation is a cream mask, and step (5) specifically includes: Heat the oil phase to 75-80°C, and heat the aqueous phase containing the fermentation concentrate to 75-80°C; add the aqueous phase to the oil phase and homogenize and emulsify for 5-10 minutes; cool to 40-45°C, add the active ingredient, and stir evenly; cool to room temperature and fill.

[0014] Thirdly, a body constitution identification and facial mask product recommendation system, applied to the aforementioned facial mask, includes: The constitution identification module is used to obtain the user's nine constitution scores and main constitution through five-dimensional assessment, which includes AI tongue diagnosis, facial diagnosis and intelligent consultation. The product recommendation module is used to automatically recommend corresponding face mask products based on the judged body type. The solution module is used to provide suggestions on the frequency and combination of face masks based on the user's mixed skin type. The synergistic conditioning module is used to combine the internal nine-body dietary therapy plan with external facial mask products to achieve synergistic internal and external conditioning.

[0015] The beneficial effects of this invention are as follows: 1. This invention is based on the theory of nine constitution types in Traditional Chinese Medicine (TCM). It establishes a complete correspondence map between constitution, skin manifestation, and efficacy for each of the nine constitution types—balanced, qi-deficient, yang-deficient, yin-deficient, phlegm-dampness, damp-heat, blood stasis, qi stagnation, and special constitution—and their corresponding skin problems. Based on this map, nine differentiated mask formulas are designed following the principles of monarch-minister-assistant-guide drug combination, achieving for the first time full coverage and precise targeted care for all nine TCM constitution types. In each formula, the monarch-minister level plays a leading role in addressing the core skin problems of each constitution; the assistant-minister level assists the monarch-minister level to enhance efficacy and address secondary skin problems; and the adjuvant-minister level regulates the imbalance of the monarch-minister-assistant drug properties and addresses auxiliary needs such as moisturizing and soothing. This allows the drug levels to guide the transdermal absorption of active ingredients and harmonize the various drugs, with the four levels working synergistically to form a complete efficacy network. Simultaneously, this invention is equipped with a body constitution identification and product recommendation system based on five-dimensional assessment. Through AI tongue diagnosis, facial diagnosis and intelligent consultation, it comprehensively determines the user's body constitution type and combined constitution, automatically matches and recommends corresponding face mask products and provides personalized usage frequency and matching schemes, forming an intelligent closed loop from body constitution identification to product recommendation, realizing true "body constitution identification and mask application, one prescription per person" precise skin care. 2. This invention introduces probiotic fermentation technology into the preparation process of Jiuti facial masks. Through microbial fermentation, the raw materials of traditional Chinese medicine are biotransformed. Extracellular enzymes such as cellulase and protease secreted by microorganisms during metabolism effectively decompose plant cell walls, allowing the active ingredients, originally bound to the cell walls, to be fully released. Simultaneously, the metabolic activities of microorganisms biodegrade macromolecules in the raw materials, transforming them into smaller, more easily absorbed active ingredients. During fermentation, the microorganisms themselves also produce new active substances such as organic acids, bacterial cellulose, and polypeptides, endowing the fermented products with additional moisturizing and skin microecological regulation functions. In terms of the preparation process, this invention employs a pretreatment method combining low-temperature drying and ultra-fine pulverization. This effectively protects heat-sensitive active ingredients while significantly increasing the contact area between the raw materials, fermentation medium, and microorganisms, thus improving fermentation efficiency. The active ingredients in the fermentation broth are collected through low-temperature vacuum concentration, further avoiding the damage to the structure of active substances caused by high temperatures. Therefore, the fermented facial mask product has a richer content of active ingredients than products prepared using traditional extraction processes. The skin permeability and bioavailability of each active ingredient are significantly improved, and the product's effects in anti-oxidation, moisturizing, nourishing, and barrier repair are significantly enhanced. Furthermore, the decomposition of potential allergens such as macromolecular proteins by microorganisms during fermentation can reduce the allergenicity of the product. The organic acids produced by fermentation metabolism endow the system with natural preservative capabilities, enabling the product to maintain good preservative efficacy even with reduced chemical preservatives, thus improving the product's safety and gentleness. This invention also links the external nine-body mask with the internal nine-body dietary therapy program, forming a synergistic system of "body constitution identification, internal conditioning, and external mask" for internal and external conditioning. External application improves local skin condition, while internal administration regulates overall body constitution imbalances. The synergistic effect of the two, compared to external application or internal administration alone, can improve skin problems from both the root cause and local levels simultaneously, significantly enhancing the overall and lasting effects of skincare. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall technical framework of the present invention. Detailed Implementation

[0017] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0018] like Figure 1 The image shows a "principal-assistant-adjuvant" facial mask based on nine body constitution types and bio-fermentation technology, along with its preparation method.

[0019] The present invention will be further described in detail below with reference to embodiments, comparative examples, and experimental examples, but the implementation of the present invention is not limited thereto. In the following embodiments, the weight parts and weight parts and percentages are all weight ratio relationships. All raw materials are cosmetic raw materials that meet the requirements of the "Cosmetic Safety Technical Specifications" (2015 edition) and the "Catalogue of Used Cosmetic Raw Materials", or are substances listed in the national catalogue of food and medicine homology. All probiotic strains can be obtained through commercial channels.

[0020] Example 1: Preparation of a Qi-replenishing and brightening bio-mask (sheet type) for those with Qi deficiency 1. Raw material formula (parts by weight)

[0021] 2. Fermentation process Step 1: Raw material pretreatment Ginseng, astragalus, angelica, poria, and licorice were individually cleaned to remove impurities, washed 2-3 times with purified water, and dried in a 50℃ low-temperature drying oven for 6 hours until the moisture content was ≤8%. The dried herbs were then separately fed into an ultra-micro pulverizer and pulverized to a particle size ≤20μm (approximately 600-800 mesh). The herbs were weighed and mixed according to the above-mentioned principal, assistant, adjuvant, and guide ratios to obtain 100 parts by weight of mixed powder.

[0022] Step 2: Preparation of Fermentation Substrate Add 100 parts by weight of the mixed drug powder to 800 parts by weight of purified water (material-to-liquid ratio 1:8), add 20 parts by weight of glucose (2%) as a carbon source, and add 5 parts by weight of yeast powder (0.5%) as a nitrogen source. Stir well and adjust the pH to 6.0 with 1 mol / L sodium hydroxide solution or 1 mol / L hydrochloric acid solution. Place the above fermentation substrate in a fermenter, sterilize at 121°C for 15 minutes, cool to 30°C, and set aside for later use.

[0023] Step 3: Inoculation and Fermentation Saccharomyces cerevisiae was inoculated into YPD liquid medium and activated by incubation at 30°C and 150 rpm for 18-24 hours until OD (outlet count) was reached. 600 The pH value should reach 1.0-1.5. Inoculate the activated brewer's yeast culture at a 3% inoculum (i.e., add 3 mL of culture to every 100 mL of fermentation substrate) into the fermentation substrate obtained in step two. Maintain the fermentation temperature at 30℃ for 48 hours. During fermentation, maintain the pH between 5.5 and 6.5 with a 0.1 mol / L sodium hydroxide solution, and control the dissolved oxygen content at 20%-30% (achieved by adjusting the stirring speed and aeration rate).

[0024] Step 4: Fermentation broth treatment After fermentation, the fermentation product was centrifuged at 4000 rpm for 15 minutes, and the supernatant was collected and filtered through a 0.45 μm microporous membrane to remove bacterial cells and insoluble residues. The filtrate was placed in a rotary evaporator and concentrated under low-temperature vacuum at ≤60℃ until the relative density of the concentrate was 1.10 (measured at 25℃), thus obtaining the fermentation concentrate.

[0025] 3. Preparation of the serum (per 100 parts by weight) Add the following components to the mixing tank in the following order:

[0026] Preparation process: Add deionized water, glycerin, and 1,3-butanediol to a preparation vessel, stir and heat to 75°C; slowly add carbomer 940, homogenize and stir for 10 minutes until completely dissolved; cool to 45°C, add fermentation concentrate and nicotinamide, stir evenly; continue to cool to 35°C, adjust pH to 5.8-6.2 with triethanolamine, stir evenly to obtain the essence.

[0027] 4. Sheet-type facial mask forming Select Tencel membrane fabric (30g / m²) 2 Cut the mask sheet to the appropriate face size. Soak the mask sheet in the above essence for 6 hours, allowing each mask sheet to fully absorb approximately 25-30mL of essence. Remove the mask sheet, fold it, place it in an aluminum foil bag, and seal it to obtain the Qi-replenishing and brightening bio-mask product for those with Qi deficiency.

[0028] Example 2: Preparation of a Phlegm-Dampness Type Oil-Controlling and Acne-Clearing Bio-Mask (Topical Application) 1. Raw material formula (parts by weight)

[0029] 2. Fermentation process The raw material pretreatment, fermentation substrate preparation, inoculation fermentation and fermentation broth treatment were carried out according to the method of Example 1, with the following differences: (1) Coix seed, Poria cocos, Salvia miltiorrhiza and Glycyrrhiza uralensis were weighed and mixed according to the above ratio; (2) The material-to-liquid ratio was 1:8; (3) The carbon source was glucose, with an addition amount of 2%; the nitrogen source was yeast powder, with an addition amount of 0.5%; the sterilization conditions were sterilization at 121℃ for 15 minutes; (4) the fermentation time was shortened to 36 hours; (5) Bacillus subtilis was selected as the fermentation strain, with an inoculation amount of 3% and a fermentation temperature of 37℃.

[0030] 3. Preparation of ointment (per 100 parts by weight)

[0031] Preparation process: Add sodium alginate to deionized water, stir and heat to 75°C to dissolve it completely; cool to 50°C, add kaolin and glycerin, and stir homogenously for 10 minutes; cool to 45°C, add fermentation concentrate, tea tree oil and salicylic acid, and stir evenly; continue stirring and cool to room temperature to obtain a uniform paste.

[0032] 4. Filling The above-mentioned paste is filled into vacuum bottles or tubes, each containing 30g or 50g, and sealed to obtain the phlegm-dampness-controlling and acne-clearing biological facial mask product.

[0033] Example 3: Preparation of a skin-brightening and spot-fading bio-mask (sheet type) for blood stasis constitution 1. Raw material formula (parts by weight)

[0034] 2. Fermentation process The method of Example 1 was followed, except that: (1) Salvia miltiorrhiza, Angelica sinensis, peach kernel and licorice were weighed and mixed according to the above ratio; (2) the material-liquid ratio was 1:10; (3) the carbon source was glucose, with an addition amount of 3%; the nitrogen source was yeast powder, with an addition amount of 0.8%; the sterilization conditions were sterilization at 121℃ for 20 minutes; (4) the fermentation temperature was 32℃ and the fermentation time was 48 hours; (5) the fermentation strain was a mixture of brewer's yeast and lactic acid bacteria (volume ratio 1:1), with a total inoculum of 3%.

[0035] 3. Essence preparation and molding The essence and sheet mask were prepared according to the method in Example 1, with the following differences: the amount of fermentation concentrate used was 15 parts by weight, the amount of niacinamide was 3 parts by weight, and vitamin C was added when the temperature was lowered to 40°C to avoid high-temperature degradation. A bio-cellulose sheet was used for the mask, and the soaking time was 6 hours.

[0036] Example 4: Preparation of Yin-nourishing and anti-wrinkle bio-mask (sheet type) for Yin deficiency constitution 1. Raw material formula (parts by weight)

[0037] 2. Fermentation process The method of Example 1 was followed, except that: (1) Polygonatum odoratum, Ophiopogon japonicus, lily bulb and licorice were weighed and mixed according to the above ratio; (2) The ratio of material to liquid was 1:6; (3) The carbon source was glucose, with an addition amount of 2%; the nitrogen source was peptone, with an addition amount of 0.6%; the sterilization conditions were sterilization at 121℃ for 18 minutes; (4) the fermentation temperature was 30℃ and the fermentation time was 48 hours; (5) the fermentation strain was lactic acid bacteria (Lactobacillus), with an inoculum amount of 4%.

[0038] 3. Essence preparation and molding The essence was prepared according to the method in Example 1, with the following differences: the amount of fermentation concentrate used was 18 parts by weight; collagen peptides and ceramide NP were added when the temperature was lowered to 42°C; and the membrane was made of bio-cellulose and soaked for 8 hours.

[0039] Example 5: Preparation of a Moist-Heat-Induced Anti-inflammatory and Acne-Clearing Bio-Mask (Sheet Type) 1. Raw material formula (parts by weight)

[0040] 2. Fermentation process The method of Example 1 was followed, except that: (1) Coix seed, Scutellaria baicalensis, Gardenia jasminoides, Centella asiatica and Glycyrrhiza uralensis were weighed and mixed according to the above ratio; (2) The material-liquid ratio was 1:5; (3) The carbon source was sucrose, with an addition amount of 2.5%; the nitrogen source was yeast powder, with an addition amount of 0.8%; the sterilization conditions were sterilization at 121℃ for 15 minutes; (4) the fermentation temperature was 32℃ and the fermentation time was 36 hours; (5) Bacillus subtilis was selected as the fermentation strain, with an inoculation amount of 4%.

[0041] 3. Essence preparation and molding The essence and sheet mask were prepared according to the method in Example 1, except that: azelaic acid was added when the temperature was lowered to 40°C; and Tencel sheet mask was used.

[0042] Example 6: Preparation of a Mild Nourishing and Moisturizing Bio-mask (Sheet Type) 1. Raw material formula (parts by weight)

[0043] 2. Fermentation process The method of Example 1 was followed, except that: (1) yam, wolfberry, white fungus, jujube and licorice were weighed and mixed according to the above ratio; (2) the material-liquid ratio was 1:7; (3) the carbon source was glucose, with an addition amount of 1.5%; the nitrogen source was yeast powder, with an addition amount of 0.5%; the sterilization conditions were sterilization at 121℃ for 15 minutes; (4) the fermentation temperature was 28℃ and the fermentation time was 72 hours; (5) the fermentation strain was brewer's yeast, with an inoculation amount of 2%.

[0044] 3. Essence preparation and molding The essence and sheet mask were prepared according to the method in Example 1, with the following differences: the amount of fermentation concentrate used was 12 parts by weight; sodium hyaluronate was added when the temperature dropped to 45°C; and the mask sheet was made of Tencel fabric and soaked for 6 hours.

[0045] Example 7: Preparation of a warming and rosy bio-mask (sheet type) for those with Yang deficiency 1. Raw material formula (parts by weight)

[0046] 2. Fermentation process The method of Example 1 was followed, except that: (1) Cinnamon, dried ginger, safflower, jujube and licorice were weighed and mixed according to the above ratio; (2) The ratio of material to liquid was 1:9; (3) The carbon source was sucrose, with an addition amount of 3%; the nitrogen source was peptone, with an addition amount of 1%; the sterilization conditions were sterilization at 121℃ for 20 minutes; (4) The fermentation temperature was 30℃ and the fermentation time was 48 hours; (5) The fermentation strain was a mixture of brewer's yeast and bifidobacteria (volume ratio 1:1), with a total inoculum of 3%.

[0047] 3. Essence preparation and molding The essence and sheet mask were prepared according to the method in Example 1, with the following differences: the amount of fermentation concentrate used was 15 parts by weight; vitamin E was added when the temperature dropped to 40°C; and the mask sheet was made of bio-cellulose and soaked for 6 hours.

[0048] Example 8: Preparation of a Liver-Soothing and Complexion-Revitalizing Bio-Mask (Patty Type) for Individuals with Qi Stagnation 1. Raw material formula (parts by weight)

[0049] 2. Fermentation process The method of Example 1 was followed, except that: (1) Rose, Albizia, Angelica sinensis and licorice were weighed and mixed according to the above ratio; (2) The ratio of material to liquid was 1:8; (3) The carbon source was glucose, with an addition amount of 2%; the nitrogen source was yeast powder, with an addition amount of 0.5%; the sterilization conditions were sterilization at 121℃ for 15 minutes; (4) The fermentation temperature was 30℃ and the fermentation time was 48 hours; (5) The fermentation strain was brewer's yeast, with an inoculation amount of 3%.

[0050] 3. Essence preparation and molding The essence and sheet mask were prepared according to the method in Example 1, with the following differences: the amount of fermentation concentrate used was 15 parts by weight; vitamin C was added when the temperature dropped to 40°C; sodium hyaluronate was added when the temperature dropped to 45°C; and the mask sheet was made of Tencel fabric and soaked for 6 hours.

[0051] Example 9: Preparation of a Special Anti-allergic and Soothing Bio-mask (Sheet Type) 1. Raw material formula (parts by weight)

[0052] 2. Fermentation process The method of Example 1 was followed, except that: (1) Plum, Ganoderma lucidum, lily, Centella asiatica and licorice were weighed and mixed according to the above ratio; (2) The ratio of material to liquid was 1:8; (3) The carbon source was glucose, with an addition amount of 2%; the nitrogen source was yeast powder, with an addition amount of 0.5%; the sterilization conditions were sterilization at 121℃ for 15 minutes; (4) The fermentation temperature was 30℃ and the fermentation time was 48 hours; (5) The fermentation strain was a mixture of Bifidobacterium and Lactobacillus (volume ratio 1:1), with a total inoculum amount of 3%.

[0053] 3. Essence preparation and molding The essence and sheet mask were prepared according to the method in Example 1, with the following differences: the amount of fermentation concentrate used was 15 parts by weight; ceramide NP was added when the temperature dropped to 42°C; and the mask sheet was made of bio-cellulose and soaked for 6 hours.

[0054] Example 10: Verification of Process Parameter Boundary Conditions (Phlegm-based Topical Mask) 1. Raw material formula Same as the formulation in Example 2.

[0055] 2. Fermentation process The method of Example 2 was followed, except that the following boundary parameter combinations were used: (1) the material-to-liquid ratio was 1:5 (minimum boundary value); (2) the carbon source was sucrose, with an addition amount of 1% (minimum boundary value); the nitrogen source was peptone, with an addition amount of 0.5% (minimum boundary value); (3) the sterilization conditions were sterilization at 121℃ for 15 minutes; (4) the fermentation pH was adjusted to 5.5 (minimum boundary value); (5) the inoculum amount was 1% (minimum boundary value), the fermentation temperature was 37℃, and the fermentation time was 24 hours (minimum boundary value); (6) the fermentation strain was Bacillus subtilis, with an inoculum amount of 1%.

[0056] 3. Fermentation broth treatment and paste preparation Same as Example 2.

[0057] Example 11: Verification of Process Parameter Boundary Conditions (Yin Deficiency Constitution Sheet Mask) 1. Raw material formula Same as the formulation in Example 4.

[0058] 2. Fermentation process The method of Example 4 was followed, except that the following boundary parameter combinations were used: (1) the material-to-liquid ratio was 1:10 (maximum boundary value); (2) the carbon source was glucose, with an addition amount of 3% (maximum boundary value); the nitrogen source was yeast powder, with an addition amount of 1% (maximum boundary value); (3) the sterilization conditions were sterilization at 121℃ for 20 minutes; (4) the fermentation pH was adjusted to 6.5 (maximum boundary value); (5) the inoculum amount was 5% (maximum boundary value), the fermentation temperature was 37℃, and the fermentation time was 72 hours (maximum boundary value); (6) lactic acid bacteria were selected as the fermentation strain, with an inoculum amount of 5%.

[0059] 3. Fermentation broth treatment and essence preparation Same as Example 4.

[0060] Example 12: Detailed Implementation of the Body Constitution Identification and Facial Mask Product Recommendation System 1. System Architecture The body constitution identification and facial mask product recommendation system that accompanies this invention is an intelligent recommendation system based on a client-server architecture, comprising three components: a client (mobile APP or WeChat mini program), a server (cloud data processing and reasoning platform), and a database (user profile database, body constitution assessment model database, and product knowledge graph database).

[0061] 2. Specific implementation method of the body constitution identification module 2.1 Specific Data Acquisition and Recognition Methods for AI Tongue Diagnosis Users take standard tongue photos through the client (requirements: under natural light, the tongue is naturally extended, the tongue surface is complete and clear, without coating, and beauty filters are turned off when taking the photo), and upload the photos to the server.

[0062] Tongue image recognition uses a deep learning algorithm. The specific recognition steps are as follows: (1) Tongue segmentation: The U-Net semantic segmentation model is used to segment the tongue region of the tongue image. The model is trained using a TCM tongue image dataset containing 5000 labeled tongue contours. The input image size is 224×224×3 (RGB), and the output is a tongue region mask. The segmentation accuracy IoU≥0.95.

[0063] (2) Tongue color feature extraction: Within the segmented tongue region, the RGB color space features (mean, standard deviation, histogram distribution) and HSV color space features of the tongue body are extracted. The criteria for tongue color determination are as follows:

[0064] (3) Tongue coating feature extraction: The texture features (contrast, energy, entropy, correlation) of the tongue coating are extracted using the gray co-occurrence matrix (GLCM), and the tongue coating type is determined by combining the color features (thin white coating, thin yellow coating, thick white coating, thick yellow coating, greasy coating, etc.).

[0065] (4) Tongue feature extraction: The edge detection algorithm (Canny operator) is used to extract the tongue contour and calculate the morphological parameters such as the length-to-width ratio, tongue area, and number of teeth marks, which are used to determine features such as stigmatic tongue and swollen tongue.

[0066] 2.2 Specific Data Collection and Recognition Methods for AI-Assisted Face Consultation Users take standard facial photos through the client (requirements: natural light, facing straight ahead, clear and complete face, no makeup, beauty filters turned off during shooting), and upload the photos to the server.

[0067] Facial analysis employs a multi-task cascaded convolutional neural network (MTCNN) for face detection and key point localization. The specific recognition steps are as follows: (1) Facial region segmentation: The MTCNN model was used for facial detection, and 68 key facial feature points (including eyebrows, eyes, nose, mouth and jaw contour) were located.

[0068] (2) Facial feature extraction: Skin color features (L*a*b*color space) are extracted from five regions: forehead, cheeks, nose tip, and chin. The facial color judgment criteria are as follows:

[0069] (3) Facial feature recognition: Detect and quantify the regional distribution of the following skin problems: the proportion of pigmentation area (threshold segmentation method), the number of acne lesions (morphological detection), the size of pores (local binary mode LBP texture analysis), and the distribution of red blood vessels (red channel enhanced detection).

[0070] 2.3 Specific Implementation Methods of Intelligent Consultation The intelligent consultation module uses a knowledge graph-based dialogue system to obtain the user's symptom information through the following steps: (1) Standard consultation questionnaire: The system has a built-in 60-question constitution assessment scale based on the "Classification and Determination of Traditional Chinese Medicine Constitution" (GB / T 46939-2025), covering the following eight dimensions:

[0071] (2) Adaptive Questionnaire Reduction: Using computerized adaptive testing (CAT) technology, subsequent questions are dynamically adjusted based on the results of the questions already answered by the user, reducing the number of questions from 60 to 20-25 while ensuring the accuracy of judgment (≥90%).

[0072] (3) Natural language consultation: The system provides an additional open natural language input interface, allowing users to describe their symptoms via voice or text. The system uses the BERT model for symptom entity recognition and semantic understanding, extracting key symptom information for auxiliary judgment.

[0073] 2.4 Calculation Methods for Nine Types of Physical Fitness Scores Based on data from three dimensions—tongue diagnosis, facial diagnosis, and intelligent consultation—scores for each constitution type are calculated using the following weighted averages: Weighting of each dimension:

[0074] Formulas for each physical fitness level: Let the tongue diagnosis score of the i-th constitution be Tᵢ (out of 100, weighted by the similarity of tongue color, tongue coating, and tongue shape characteristics to the standard constitution), the facial diagnosis score be Fᵢ (out of 100, weighted by the similarity of facial color and skin characteristics to the standard constitution), and the inquiry score be Qᵢ (out of 100, converted from the GB / T 46939-2025 scale score). Then the comprehensive score Sᵢ of the i-th constitution is: Sᵢ=0.30×Tᵢ+0.25×Fᵢ+0.45×Qᵢ The rules for judging the physical condition of the chief judge are as follows: (1) If there is a certain constitution type Sᵢ≥70 points, and the score is at least 10 points higher than the scores of all other constitution types, then the constitution type is determined to be the main constitution type; (2) If the difference between the highest and second highest scores is less than 10 points, it is judged as a mixed constitution. The highest score is selected as the main criterion, and the second highest score is marked as a mixed constitution. (3) If the scores of all constitution types are <60, the constitution is judged as "tends to be balanced" and the main constitution is recorded as balanced. (4) If the highest score physique S_max ≥ 80 points, it is judged as a "typical" physique, and the recommended core usage frequency is once a day; (5) If 70≤S_max<80, it is judged as "prone" constitution, and the recommended core usage frequency is once every other day; (6) If 60≤S_max<70 points, it is judged as "predisposed" constitution, and the recommended core usage frequency is twice a week.

[0075] Rules for determining and treating concurrent constitution: (1) If a person with mixed constitution scores S_j≥65 points and the difference between the main constitution score and the main constitution score is between 10 and 20 points, it is judged as "significant mixed constitution". It is recommended to use the main constitution mask and the mixed constitution mask alternately (ratio 2:1). (2) If a person with mixed constitution scores S_j≥60 and<65, it is judged as “mild mixed constitution”. It is recommended to use the main constitution mask for 5 consecutive days and then use the mixed constitution mask for 1 day. (3) If there are multiple concurrent constitutions (≥ 2), the 2 concurrent constitutions with the highest scores are selected, and a rotation scheme of "mainly using the mask for the primary-determined constitution, supplemented by masks for concurrent constitutions" is recommended. Specifically: the mask for the primary-determined constitution is used for 3 days, the mask for the first concurrent constitution is used for 1 day, the mask for the primary-determined constitution is used for 3 days, the mask for the second concurrent constitution is used for 1 day, and the cycle is repeated.

[0076] 3. Specific Implementation of the Product Recommendation Module Product recommendation mapping rules:

[0077] 4. Specific Implementation of the Synergistic Conditioning Module The system has an embedded knowledge base of nine-constitution diet regimens, and recommends corresponding internal diet regimens according to the user's primary-determined constitution and concurrent constitutions, so as to achieve the synergy of "internal conditioning and external nourishment":

[0078] 5. System Usage Procedure The complete procedure for a user to use the supporting system of the present invention is as follows: (1) A user registers and logs in through a client (WeChat Mini Program or APP); (2) The user completes AI face diagnosis (taking facial photos), AI tongue diagnosis (taking tongue photos), and intelligent inquiry (completing an adaptive questionnaire) in sequence according to the system guidance; (3) After receiving all data, the server side of the system completes comprehensive calculation within 5 to 10 seconds and generates the *Nine Constitutions Identification Report*, which includes: radar chart scores of nine constitutions, primary-determined constitution and its score, concurrent constitutions and their scores, and description of constitution characteristics; (4) The system automatically jumps to the product recommendation page according to the primary-determined constitution, and displays the corresponding product information of nine-constitution biological masks (product name, efficacy description, usage method, ingredient list); (5) If the user has concurrent constitutions, the system additionally displays the "usage scheme for concurrent constitutions" and provides suggestions on rotation frequency and combination of masks; (6) The system synchronously pushes the corresponding internal diet regimen, forming a complete closed-loop traditional Chinese medicine beauty trinity of "constitution identification report, external mask regimen, and internal diet regimen"; (7) The user's usage records and constitution change data are automatically stored in the user profile database, and the system automatically reminds the user to take a re-test after 28 consecutive days of use, and dynamically updates the constitution score and product recommendation.

[0079] Comparative Example 1 (Mask with Unfermented Extract) According to the raw material formula of Example 1 (5 parts of Ginseng Radix et Rhizoma, 8 parts of Astragali Radix, 5 parts of Angelicae Sinensis Radix, 5 parts of Poria, 2 parts of nicotinamide, 2 parts of Glycyrrhizae Radix et Rhizoma), the traditional water decoction extraction method is used instead of the biological fermentation method: After ginseng, astragalus, angelica, poria, and licorice were selected, washed, and dried, they were not subjected to ultrafine grinding. Instead, they were directly mixed and soaked in 10 times the amount of purified water for 1 hour. Then, they were decocted twice at 100°C for 1.5 hours each time. The filtrates were combined and concentrated at 60°C to a relative density of 1.10 to obtain a traditional Chinese medicine extract. The extract was then compounded with nicotinamide and a mask matrix in the same proportions as in Example 1 to prepare a sheet mask. The remaining steps were the same as in Example 1.

[0080] Comparative Example 2 (mask without the proper formulation of the principal, assistant, adjuvant, and guide ingredients) According to the raw material composition and dosage of Example 1 (5 parts ginseng, 8 parts astragalus, 5 parts angelica, 5 parts poria, 2 parts nicotinamide, 2 parts licorice), but without distinguishing the principal, assistant, adjuvant and guide levels, all raw materials are regarded as a single mixed component in equal amounts, and the same bio-fermentation process as in Example 1 is used to prepare the sheet mask.

[0081] Comparative Example 3 (Non-Physical Type Universal Mask) Following the conventional Chinese herbal mask formula design, 5 parts of Angelica dahurica, 5 parts of Poria cocos, 5 parts of Paeonia lactiflora, 5 parts of Atractylodes macrocephala, 5 parts of Bletilla striata, and 2 parts of Glycyrrhiza uralensis were selected. The sheet mask was prepared using the same bio-fermentation process as in Example 1, without any formula design targeting specific body constitutions.

[0082] Experimental Example 1: Comparative Determination of Active Ingredient Content Before and After Fermentation 1. Experimental Objective Compare the changes in the content of the main active ingredients in the formulation raw materials described in Example 1 before and after bio-fermentation.

[0083] 2. Experimental Materials Experimental group: Fermentation concentrate obtained in Example 1 (fermentation group). Control group: Traditional Chinese medicine extract obtained in Comparative Example 1 (unfermented extract group), prepared by the same method as Comparative Example 1, i.e., extract obtained by traditional water decoction extraction.

[0084] 3. Experimental Methods The contents of three representative active ingredients, ginsenoside Rg1, astragaloside A, and glycyrrhizic acid, were determined by high performance liquid chromatography (HPLC).

[0085] Sample pretreatment: Unfermented group: Take 1g of mixed powder, add 10mL of 70% methanol, extract by ultrasonication for 30 minutes, filter, make up to 10mL, filter through a 0.45μm filter membrane, and wait for testing.

[0086] Fermentation group: Take 1 mL of fermentation concentrate, add 9 mL of 70% methanol, extract by ultrasonication for 15 minutes, filter, make up to 10 mL, filter through a 0.45 μm filter membrane, and wait for analysis.

[0087] Chromatographic conditions: C18 column (4.6 mm × 250 mm, 5 μm); mobile phase A was acetonitrile, and mobile phase B was 0.1% phosphoric acid aqueous solution, with gradient elution; detection wavelengths were 203 nm (ginsenoside Rg1), 200 nm (astragaloside A), and 254 nm (glycyrrhizic acid); flow rate was 1.0 mL / min; column temperature was 30 ℃; and injection volume was 10 μL.

[0088] 4. Experimental Results

[0089] Note: Data are expressed as mean ± standard deviation, n=3; P-values ​​were obtained using an independent samples t-test.

[0090] 5. Results Analysis After fermentation with Saccharomyces cerevisiae, the content of ginsenoside Rg1 increased by 90.2%, astragaloside A by 75.6%, and glycyrrhizic acid by 63.4%, all with highly statistically significant differences (P<0.01). This indicates that microbial fermentation can effectively decompose plant cell walls, release bound active ingredients, and significantly improve the dissolution rate of active ingredients in traditional Chinese medicine raw materials.

[0091] Experiment Example 2: Comparative Experiment of In Vitro Antioxidant Activity 1. Experimental Objective The differences in in vitro antioxidant activity between Example 1 (fermentation group) and Comparative Example 1 (unfermented extract group) of the present invention were compared.

[0092] 2. Experimental Methods 2.1 Determination of DPPH free radical scavenging rate The fermentation concentrate obtained in Example 1 and the traditional Chinese medicine extract obtained in Comparative Example 1 were diluted with deionized water to a series of concentrations (0.5, 1.0, 2.0, 4.0, 8.0 mg / mL). 2 mL of each concentration sample solution was added to 2 mL of 0.1 mmol / L DPPH ethanol solution, mixed well, and reacted at room temperature in the dark for 30 minutes. The absorbance A1 was measured at 517 nm. 2 mL of deionized water was used as a blank control instead of the sample solution, and the absorbance A0 was measured. 2 mL of anhydrous ethanol was used as the sample background instead of the DPPH solution, and the absorbance A2 was measured. The DPPH free radical scavenging rate was calculated. Clearance rate (%) = [1 - (A1 - A2) / A0] × 100% 2.2 Determination of Total Reducing Power The potassium ferricyanide reduction method was used. Take 1 mL of sample solutions of different concentrations, add 2.5 mL of 0.2 mol / L phosphate buffer (pH 6.6) and 2.5 mL of 1% potassium ferricyanide solution, mix well, and react in a 50℃ water bath for 20 minutes. Add 2.5 mL of 10% trichloroacetic acid solution, mix well, centrifuge at 3000 rpm for 10 minutes, take 2.5 mL of the supernatant, add 2.5 mL of deionized water and 0.5 mL of 0.1% ferric chloride solution, mix well, and measure the absorbance at 700 nm. A higher absorbance value indicates stronger reducing power.

[0093] 3. Experimental Results

[0094] Note: Data are expressed as mean ± standard deviation, n=3; P-values ​​were obtained using an independent samples t-test.

[0095] IC50 of DPPH free radical scavenging rate in fermentation group 50 The value was 1.53 mg / mL, and the IC50 value for the unfermented group was 1.53 50 The value was 2.87 mg / mL. At a concentration of 4.0 mg / mL, the total reducing power absorbance (AL) of the fermentation group was... 700 The mean value of the control group was 0.892±0.045, while that of the unfermented group was 0.615±0.038. The difference between the two groups was statistically significant (P<0.01).

[0096] 4. Results Analysis The fermentation concentrate obtained in Example 1 of this invention showed significantly higher DPPH radical scavenging rate and total reducing power at various concentrations than the unfermented extract of Comparative Example 1 (P<0.01). 50 The value decreased by 46.7%. This indicates that bio-fermentation technology significantly improves the antioxidant activity of facial mask products.

[0097] Experiment Example 3: Comparative Experiment on Moisturizing Effects on Human Skin 1. Experimental Objective The moisturizing effect of Embodiment 1 of the present invention (Qi-deficient constitution Qi-replenishing and complexion-brightening bio-mask) was evaluated.

[0098] 2. Experimental Methods 2.1 Subjects Thirty healthy female volunteers, aged 25-50 years (mean age 36.5 ± 7.2 years), who met the criteria for Qi deficiency constitution, were recruited. They had no skin diseases and had not used any skincare products on the test area for two weeks prior to the trial. All participants signed informed consent forms.

[0099] 2.2 Experimental Design A self-controlled before-and-after design was used. The left side of the subject's face was the experimental area (using one mask from the example), and the right side was the control area (using one mask from the comparison). Subjects used the corresponding mask once a day for 15 minutes each time, for 28 consecutive days.

[0100] 2.3 Detection Indicators Skin stratum corneum moisture content was measured using a Corneometer® CM 825 before use (D0), immediately after use (D0), 7 days after use (D7), 14 days after use (D14), and 28 days after use (D28). Transepidermal water loss (TEWL) values ​​were measured using a Tewameter® TM 300. Each measurement was performed after acclimatization for 30 minutes in a constant temperature and humidity environment (temperature 22±1℃, relative humidity 50±5%), and each test point was measured three times, with the average value taken.

[0101] 3. Experimental Results

[0102] Note: Data are expressed as mean ± standard deviation, n=30; the p-value between groups was determined by paired samples t-test.

[0103] 4. Results Analysis After using one mask according to the embodiments of the present invention, the skin moisture content increased from 38.5 au at D0 to 54.1 au at D28, an increase of 40.5% (P<0.01); the TEWL value increased from 16.8 g / h·m at D0. 2 Reduced to 10.1 g / h·m at D28 2 The skin moisture content decreased by 39.9% (P<0.01). In contrast, after using one mask in the comparative example, skin moisture content increased by only 20.9%, and the TEWL value decreased by only 18.2%. The differences between the two groups at each time point were statistically significant (P<0.01). The results indicate that the bio-fermented mask in the example was significantly superior to the unfermented comparative mask in increasing skin moisture content and repairing skin barrier function.

[0104] Experiment Example 4: Comparative Experiment on Skin Whitening Effects on Humans 1. Experimental Objective The whitening and spot-fading effects of Embodiment 3 of the present invention (blood stasis-reducing and spot-fading bio-mask) were evaluated.

[0105] 2. Experimental Methods 2.1 Subjects Thirty healthy female volunteers, aged 30-55 years (mean age 42.3 ± 6.8 years), who met the criteria for blood stasis constitution and had facial pigmentation or melasma, were recruited. Exclusion criteria included: pregnant or breastfeeding women; women with active facial skin diseases; and women who had used whitening or spot-removing products within the past 3 months. All participants signed informed consent forms.

[0106] 2.2 Experimental Design A randomized, double-blind, self-controlled design was employed. Subjects were randomly assigned to two groups: an experimental group (using the mask from Example 3) and a control group (using the comparative mask, i.e., a mask prepared from the unfermented extract), with 15 subjects in each group. Subjects used the corresponding mask once daily for 15 minutes each time, for 56 consecutive days (8 weeks).

[0107] 2.3 Detection Indicators At the following times: before use (D0), after 28 days of use (D28), and after 56 days of use (D56): Skin lightness (L*) and melanin index (MI) were measured using a Chromameter® CR-400 skin colorimeter. Standard facial images were captured using the VISIA® facial image analysis system to analyze the area ratio and density of pigmentation spots.

[0108] 3. Experimental Results

[0109] Note: Data are expressed as mean ± standard deviation, n=15; the p-value between groups was determined by independent samples t-test.

[0110] 4. Results Analysis After using the mask of Example 3 for 56 days, the skin's L* value increased from 58.2 to 65.3, an increase of 12.2% (P<0.01); the melanin index MI decreased from 185.6 to 148.7, a decrease of 19.9% ​​(P<0.01); and the proportion of pigmented area decreased from 12.5% ​​to 7.2%, a reduction of 42.4% (P<0.01). In contrast, the improvement in all indicators in the control group was significantly lower than that in the experimental group (P<0.05). The results indicate that the bio-fermentation-treated mask of Example 3 of this invention has significant effects in brightening skin tone, reducing melanin, and fading pigmented areas.

[0111] Experimental Example 5: Skin Safety Evaluation (Human Patch Experiment) 1. Experimental Objective Evaluate the skin safety of the facial mask product of this invention.

[0112] 2. Experimental Methods Following the "Human Skin Patch Test" method in the *Cosmetic Safety Technical Specifications* (2015 edition), 30 healthy volunteers (half male and half female, aged 20-60 years, mean age 38.2 ± 10.5 years) were selected. 0.03 mL of each of the mask essences obtained in Examples 1 to 9 was added to the patch test chamber and applied to the normal skin on both sides of the spine on the back of the subjects. Deionized water was used as a blank control. After 24 hours of continuous application, the patch test chamber was removed. Skin reactions were observed at 0.5 hours, 24 hours, and 48 hours after removal and scored according to the following criteria:

[0113] 3. Experimental Results

[0114] Note: The data in the table represent the highest response levels of the 30 subjects.

[0115] 4. Results Analysis No skin irritation reactions such as erythema or edema were observed in any of the subjects at any observation time point during the patch test, and all subjects received a score of 0. The results indicate that the facial mask products of Examples 1 to 9 of this invention are non-irritating to the skin and safe to use.

[0116] Experiment Example 6: Verification of the Efficacy Corresponding to Nine Body Constitutions -- In Vitro Tyrosinase Inhibition Experiment 1. Experimental Objective The tyrosinase inhibitory activity of the nine body type masks of the present invention related to whitening and spot fading (Example 3 Blood Stasis Constitution, Example 8 Qi Stagnation Constitution) was compared, with Comparative Example 3 (non-body type suitable general type mask) as a control.

[0117] 2. Experimental Methods Fermentation concentrates from Examples 3 and 8, and from Comparative Example 3, were diluted with deionized water to a series of concentrations (0.5, 1.0, 2.0, 4.0, and 8.0 mg / mL). Following the literature method, the tyrosinase inhibition rate was determined using L-DOPA as a substrate. In a 96-well plate, add 80 μL of phosphate buffer (pH 6.8), 40 μL of sample solution, and 40 μL of tyrosinase solution (200 U / mL) to each well. Mix well and incubate at 37°C for 10 minutes. Then add 40 μL of 2.5 mmol / L L-DOPA solution and incubate at 37°C for 20 minutes. Measure the absorbance at 475 nm. Use deionized water instead of the sample as a blank control and calculate the tyrosinase inhibition rate. Inhibition rate (%) = [1 - (Sample A - Sample A background) / (Blank A - Blank A background)] × 100% 3. Experimental Results

[0118]

[0119] 4. Results Analysis IC in Example 3 50 The value was 0.92 mg / mL, and the IC50 value in Example 8 was... 50 The value was 1.38 mg / mL, and the IC50 of Comparative Example 3 was... 50 The value was 3.86 mg / mL. The masks of Examples 3 and 8, designed for blood stasis and qi stagnation constitutions respectively, showed significantly better tyrosinase inhibitory activity than the unsuitable comparative example 3 (P<0.01), IC50. 50 The values ​​were 76.2% and 64.2% lower than those of the control group, respectively. This indicates that the targeted formula designed based on body constitution classification and the principle of monarch-minister-assistant-guide combination has a significant synergistic effect in inhibiting melanin production.

[0120] Experiment Example 7: Comprehensive Evaluation of the Efficacy of Face Masks for Nine Body Types 1. Experimental Objective The overall evaluation of the nine body type face masks of this invention improves the skin problems of the corresponding body types.

[0121] 2. Experimental Methods Twenty healthy volunteers meeting each of the criteria for different body constitutions were recruited (a total of 180 volunteers, aged 25-55 years, with no statistically significant age difference between groups). Nine different body constitution masks (Examples 1 to 9) were used according to the corresponding constitution, applied once daily for 15 minutes each time, for 28 consecutive days. Skin tests were conducted before use (D0) and after use (D28), with test indicators set according to the core skin problems of different body constitutions.

[0122] 3. Experimental Results

[0123] 4. Results Analysis The nine types of body type masks of this invention showed significant improvement effects on the core skin problems corresponding to their respective body types (P<0.01 or P<0.05), with improvement rates of over 10% for the core indicators of each body type, and subject self-satisfaction rates exceeding 85%. These results demonstrate that the nine-type body type mask series, designed based on the concept of "body type-specific mask application," can accurately and effectively improve skin problems in different body types, validating the scientific validity and effectiveness of the "body type-skin problem-mask efficacy" correspondence map of this invention.

[0124] Experiment Example 8: Fermentation Process Stability Verification Experiment 1. Experimental Objective To verify the batch-to-batch consistency and stability of the bio-fermentation process of this invention.

[0125] 2. Experimental Methods Following the process parameters of Example 1, three batches (batch numbers: 20260601, 20260602, and 20260603) of fermented concentrate for Qi-deficiency and brightening bio-masks were prepared consecutively. The total polysaccharide content (phenol-sulfuric acid method), total flavonoid content (AlCl3 colorimetric method), pH value, relative density, and microbial limit were determined for each batch, and the relative standard deviation (RSD) between batches was calculated.

[0126] 3. Experimental Results

[0127] 4. Results Analysis The RSD of total polysaccharide content in the three batches of fermentation concentrate was 1.36%, the RSD of total flavonoid content was 2.15%, the RSD of pH value was 0.60%, and the RSD of relative density was 0.32%, with minimal differences between batches. All batches met the requirements of the "Cosmetic Safety Technical Specifications" (2015 edition). The results indicate that the bio-fermentation preparation process described in this invention is stable and reliable, with good batch-to-batch reproducibility, and is suitable for industrial production.

[0128] Experiment Example 9: Boundary Process Parameter Verification Experiment 1. Experimental Objective Verify the feasibility and efficacy consistency of the product under the boundary values ​​of the process parameters recorded in the claims of this invention (such as material-liquid ratio of 1:5 and 1:10, pH of 5.5 and 6.5, inoculum amount of 1% and 5%, and fermentation time of 24h and 72h).

[0129] 2. Experimental Methods The formulas from Example 2 (phlegm-dampness constitution) and Example 4 (yin deficiency constitution) were selected and fermented according to different combinations of process parameters shown in the table below. A total of 6 experimental groups were set up:

[0130] The total active ingredient content (calculated as total flavonoids, mg / mL) and DPPH free radical scavenging rate (IC50) of the fermentation concentrates of each experimental group were determined. 50 Value (mg / mL).

[0131] 3. Experimental Results

[0132] Note: Data are expressed as mean ± standard deviation, n=3.

[0133] 4. Results Analysis Total flavonoid content and DPPH free radical scavenging rate IC50 in boundary value experimental groups (Group A, Group B, Group D, and Group E) 50 Compared with the central value experimental groups (Group C and Group F), the relative deviations were all between -8.2% and +0.7%, with no statistically significant difference (P>0.05). The results indicate that the process parameters described in the claims of this invention can stably prepare fermentation concentrates with good biological activity across the entire range (material-to-liquid ratio 1:5-10, pH 5.5-6.5, inoculum size 1%-5%, fermentation time 24-72h). The boundary values ​​of each parameter can achieve the technical solution of this invention and reach the expected technical effect. The parameter ranges in the claims have been fully verified and supported by experiments.

[0134] In summary, this invention achieves complete coverage of nine body constitutions. Examples 1 to 9 provide complete preparation schemes for masks of nine body constitutions, and each formula strictly follows the principle of monarch, minister, assistant and guide in the formulation. Bio-fermentation technology can significantly increase the content of active ingredients in traditional Chinese medicine raw materials (Experimental Example 1), with ginsenoside Rg1 increasing by 90.2% and astragaloside A increasing by 75.6%. The bio-fermented facial mask product was significantly superior to the unfermented control product in terms of antioxidant activity (Experimental Example 2), moisturizing effect (Experimental Example 3), and whitening effect (Experimental Example 4) (P<0.01). The facial mask product of this invention is non-irritating to the skin and safe to use (Experimental Example 5); The mask formula designed based on body constitution classification and the principle of monarch, minister, assistant and guide in the formulation has a significantly better targeted effect than general formulas that are not adapted to body constitution (Experimental Example 6). The nine different body type masks all showed significant improvement effects on the core skin problems of their respective body types (Experimental Example 7, P<0.01 or P<0.05). The preparation process of this invention is stable and reliable, with good batch-to-batch reproducibility, and is suitable for industrial production (Experimental Example 8, RSD≤2.15%). The technical effects can be stably achieved across the entire range of process parameters described in the claims of this invention, and the boundary value experiment has been verified (Experiment Example 9).

[0135] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A facial mask based on nine body constitution types and bio-fermentation technology, characterized in that... The face mask contains nine different formulas designed for nine different TCM constitutions: balanced, qi-deficient, yang-deficient, yin-deficient, phlegm-dampness, damp-heat, blood stasis, qi stagnation, and special constitution. Each of the aforementioned mask formulations comprises four levels of raw materials: principal ingredient, assistant ingredient, adjuvant ingredient, and guide ingredient. The principal ingredient plays a major role in addressing the core skin problems of the corresponding constitution and is used in the largest quantity. The assistant ingredient assists the principal ingredient in enhancing its efficacy or addresses secondary skin problems. The adjuvant ingredient adjusts the properties of the formulation or provides auxiliary effects. The guide ingredient guides the penetration of active ingredients and harmonizes the various ingredients. The traditional Chinese medicine ingredients in the mask formula are fermented with probiotics to obtain a concentrated fermentation liquid, which is then compounded with the mask matrix to form a mask preparation.

2. The facial mask according to claim 1, characterized in that, The correspondence between the nine body types and skin problems is as follows: The balanced constitution corresponds to normal skin that is moisturized and elastic; the Qi deficiency constitution corresponds to a sallow or pale complexion and loose skin; the Yang deficiency constitution corresponds to a pale complexion and cold skin; the Yin deficiency constitution corresponds to dry skin, fine lines, and flushed cheeks; the phlegm-dampness constitution corresponds to oily skin, large pores, and a tendency to develop acne; the damp-heat constitution corresponds to oily skin, acne, and itchy skin; the blood stasis constitution corresponds to a dull complexion, pigmentation, and age spots; the Qi stagnation constitution corresponds to a sallow complexion and a tendency to develop age spots; and the special constitution corresponds to sensitive skin, allergies, and telangiectasia.

3. The facial mask according to claim 1, characterized in that, The principal ingredients in the nine body constitution-specific facial mask formulas are selected from the following: balanced constitution is selected from yam, qi deficiency constitution from ginseng, yang deficiency constitution from cinnamon, yin deficiency constitution from Solomon's seal, phlegm-dampness constitution from coix seed, damp-heat constitution from coix seed, blood stasis constitution from salvia miltiorrhiza, qi stagnation constitution from rose, and special constitution from dried plum.

4. The facial mask according to claim 1, characterized in that, The probiotics are selected from one or more of the following: Saccharomyces cerevisiae, Lactobacillus, Bifidobacterium, and Bacillus subtilis.

5. The facial mask according to claim 1, characterized in that, The raw materials in the nine body type mask formulas are all cosmetic raw materials that meet the requirements of the "Cosmetic Safety Technical Specifications" and the "Catalogue of Used Cosmetic Raw Materials", or are substances listed in the national catalogue of food and medicine homology.

6. A method for preparing a facial mask as described in any one of claims 1-5, characterized in that, Includes the following steps: (1) Raw material pretreatment: The Chinese medicinal materials in each mask formula are cleaned, washed, dried at low temperature and then ultra-finely pulverized to a particle size ≤20μm. They are weighed and mixed according to the ratio of principal, assistant and adjuvant herbs to obtain mixed medicinal powder. (2) Preparation of fermentation substrate: Add the mixed powder to purified water, add carbon source and nitrogen source, adjust pH to 5.5-6.5, sterilize, and obtain fermentation substrate; (3) Inoculation and fermentation: Cool the fermentation substrate to 28-37℃, inoculate with activated probiotics, and ferment at 28-37℃ for 24-72 hours to obtain the fermentation product; (4) Fermentation broth treatment: Remove the cell bodies from the fermentation product and concentrate it under low temperature and vacuum to obtain fermentation concentrate; (5) Preparation of facial mask formulation: The fermentation concentrate is combined with the facial mask matrix to prepare a facial mask formulation; (6) Filling and packaging.

7. The preparation method according to claim 6, characterized in that, In step (2), the ratio of the mixed medicinal powder to purified water is 1:5-10, the carbon source is glucose or sucrose and the amount added is 1%-3%, the nitrogen source is yeast powder or peptone and the amount added is 0.5%-1%, and the sterilization conditions are sterilization at 121℃ for 15-20 minutes; in step (3), the inoculation amount of probiotics is 1%-5%; in step (4), the temperature of low-temperature vacuum concentration is ≤60℃, and the relative density of the fermentation concentrate is 1.05-1.

15.

8. The preparation method according to claim 6, characterized in that, The mask preparation is a sheet mask, and step (5) specifically includes: Add the fermentation concentrate and moisturizer to deionized water, stir and heat to 70-80℃; add thickener, homogenize and stir for 10-15 minutes; cool to 40-45℃, add active ingredients, stir evenly; cool to 35℃, add preservative, adjust pH to 5.5-6.5 to obtain essence; soak the mask sheet in the essence for 4-8 hours, remove, fold, pack and seal.

9. The preparation method according to claim 6, characterized in that, The mask preparation is a cream mask, and step (5) specifically includes: Heat the oil phase to 75-80°C, and heat the aqueous phase containing the fermentation concentrate to 75-80°C; add the aqueous phase to the oil phase and homogenize and emulsify for 5-10 minutes; cool to 40-45°C, add the active ingredient, and stir evenly; cool to room temperature and fill.

10. A body constitution identification and facial mask product recommendation system, applied to the facial mask according to any one of claims 1-5, characterized in that, include: The constitution identification module is used to obtain the user's nine constitution scores and main constitution through five-dimensional assessment, which includes AI tongue diagnosis, facial diagnosis and intelligent consultation. The product recommendation module is used to automatically recommend corresponding face mask products based on the judged body type. The solution module is used to provide suggestions on the frequency and combination of face masks based on the user's mixed skin type. The synergistic conditioning module is used to combine the internal nine-body dietary therapy plan with external facial mask products to achieve synergistic internal and external conditioning.

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