Skin care compositions containing whitening and penetration enhancing ingredients, methods of making and use

Abstract

The present application relates to the technical field of skin care, and provides a skin care composition containing whitening and penetration promoting components, a preparation method and application thereof, which comprises the following components: diethoxyethyl succinate, menthol lactate, rhamnolipid, subtilisin sodium, raspberry ketone glucoside, hexyl resorcinol, tranexamic acid, cetyl stearyl alcohol olivate, sorbitan olivate, thickening agent, bisabalol, eggshell membrane extract, co-surfactant, nicotinamide, skin feel adjusting component, preservative, auxiliary material, and deionized water in a balance amount. The present application has good whitening effect, can effectively reduce the irritation to the skin and the potential risk of allergy, and is helpful to promote the transdermal absorption of active ingredients.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, specifically to skincare compositions containing whitening and penetration-enhancing ingredients, their preparation methods, and applications. Background Technology

[0002] Currently, whitening products on the market typically add chemical penetration enhancers to improve their whitening effect. These chemical penetration enhancers mainly work by altering the lipid bilayer structure of the stratum corneum or by acting on proteins within stratum corneum cells to create hydration channels, thereby promoting the diffusion of active substances in the stratum corneum and increasing their transdermal absorption rate. However, conventional chemical penetration enhancers often have problems such as high irritation, damage to the skin barrier, or causing skin allergies, making it difficult to meet consumers' dual demands for skincare products that are both gentle and highly effective.

[0003] Based on this, the present invention designs a skin care composition containing whitening and penetration-enhancing ingredients, as well as a preparation method and application, to solve the above problems. Summary of the Invention

[0004] In view of the above-mentioned shortcomings of the prior art, the present invention provides a skin care composition containing whitening and penetration-enhancing ingredients, a preparation method and application thereof.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A skincare composition containing whitening and penetration-enhancing ingredients, comprising the following components: 1.3-2.0% diethoxyethyl succinate, 0.4-0.6% menthol lactate, 0.5-1.1% rhamnolipid, 0.6-1.0% sodium subtilisin, 0.5-2.0% raspberry ketone glucoside, 0.1-0.5% hexylresorcinol, 1.0-3.0% tranexamic acid, 1.0-3.0% cetearyl oleate, 0.5-2.0% sorbitan oleate, 0.2-1.0% thickener, 0.1-0.5% bisabolol, 1.6-3.5% eggshell membrane extract, 3.0-4.2% co-surfactant, 2.0-5.0% niacinamide, 2.5-8.0% skin feel modifier, 0.5-1.5% preservative, 0.1-0.5% excipients, and the balance being deionized water.

[0006] Furthermore, the thickener is selected from hydrolyzed Sclerotium rolfsii gum; the co-surfactant is selected from propylene glycol; the preservative is selected from 1,2-hexanediol; the excipient is selected from poloxamer 407; and the skin feel regulating components include polydimethylsiloxane, Butyrospermum PARKII fruit oil, and hydrogenated polydecene in a mass percentage ratio of 1.0-3.0:0.5-2.0:1.0-3.0.

[0007] Furthermore, the preparation method of the eggshell membrane extract includes: The eggshell membrane was subjected to cold and heat treatment and ultrasonic-assisted enzymatic hydrolysis in sequence to obtain an extract; The extract was concentrated to obtain the eggshell membrane extract.

[0008] Furthermore, the cold and heat treatment includes: freezing at -10 to -2°C, then heating to 90 to 95°C and holding at that temperature, followed by cooling; the ultrasound-assisted enzymatic hydrolysis includes: enzymatic hydrolysis under the action of cellulase and neutral protease, and intermittent ultrasound treatment during the enzymatic hydrolysis process.

[0009] Furthermore, the preparation method of eggshell membrane extract includes the following specific steps: (1) Raw material pretreatment Weigh the eggshell membrane, clean it, dry it to constant weight, crush it, and sieve it through a 20-50 mesh to obtain eggshell membrane powder. (2) Cold and heat treatment Freeze the eggshell membrane powder at -10 to -2℃ for 10-15 hours; let it stand at room temperature for 1.5-2.5 hours; then place it in a constant temperature water bath and heat it to 90-95℃ at 8-10℃ for 1-2 hours; finally, immediately place the material at 0-5℃ for rapid cooling for 25-30 minutes. (3) Ultrasound-assisted enzymatic hydrolysis Add eggshell membrane powder to deionized water at a material-to-liquid ratio of 1:20-23, adjust the pH to 4.5-6.5, add cellulase and neutral protease, and stir at 40-55℃ for 2-4 hours for enzymatic hydrolysis; during the enzymatic hydrolysis process, start intermittent ultrasound assistance, with an ultrasound power of 100-200W, and perform ultrasound treatment for 5 minutes every 10 minutes. (4) Filtration and rotary evaporation concentration After the ultrasonic extract was allowed to stand, it was centrifuged and filtered to collect the supernatant. The supernatant was then transferred to a rotary evaporator and concentrated under reduced pressure to obtain the eggshell membrane extract.

[0010] This invention also provides a method for preparing a skincare composition, comprising the following steps: Pretreatment of the oil phase: Diethoxyethyl succinate, menthol lactate, hexyl resorcinol, cetearyl oleate, sorbitan oleate, bisabolol, polydimethylsiloxane, shea butter, and hydrogenated polydecene are mixed, heated to 70-75℃, and stirred until dissolved and homogeneous. Preparation of aqueous phase: After heating deionized water to 60-65℃, add raspberry ketone glucoside, tranexamic acid, hydrolyzed sclerotium gum, eggshell membrane extract, propylene glycol, nicotinamide and poloxamer 407, and stir to dissolve; Slowly add the oil phase to the aqueous phase, homogenize, then add 1,2-hexanediol, rhamnolipid, and sodium subtilisin, and continue stirring. Cool to room temperature and adjust the pH to 5.8-6.2 using a citric acid / sodium citrate buffer pair; The skincare composition is obtained after vacuum degassing.

[0011] The present invention also provides an application of the described skin care composition in the preparation of whitening skin care products.

[0012] Furthermore, the amount of the skincare composition added to the whitening skincare product is 15.3-28.6% by weight.

[0013] Compared with the prior art, the beneficial effects of this invention are as follows: 1. Experimental examples of this invention show that, compared with the NC group, the melanin granules in the sample group are significantly reduced, with an inhibition rate of 48.83%, and have a better whitening effect.

[0014] 2. This invention employs a compound of menthol lactate and diethoxyethyl succinate, combined with two biosurfactants, rhamnolipid and sodium subtilis lipopeptide, and adds eggshell membrane extract to soften the stratum corneum and promote absorption, thereby constructing a penetration-enhancing system. This reduces skin irritation and potential allergic risks while promoting the transdermal absorption of active ingredients.

[0015] 3. This invention was verified by a neutrophil count reduction test. The inflammatory response in the sample group was significantly reduced compared with the model control group, indicating that the composition has a soothing effect while promoting the transdermal absorption of active ingredients. It can effectively reduce the discomfort such as redness and stinging that may be caused by traditional penetration enhancers and is suitable for sensitive skin. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0017] Figure 1 Microscopic images of melanin-stained tissue sections from an in vitro three-dimensional skin model (MelaKutis®).

[0018] Figure 2 A bar chart visualizing the skin whitening effect.

[0019] Figure 3 Results of a test on the soothing effects of zebrafish. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0021] Example 1: This example provides a skincare composition containing whitening and penetration-enhancing ingredients, prepared by: (1) Weigh the raw materials by mass percentage: Eggshell membrane extract 1.6%; Preparation method: Weigh eggshell membranes, clean them, dry them to constant weight, pulverize them, and sieve through a 35-mesh sieve to obtain eggshell membrane powder; Freeze the eggshell membrane powder at -6℃ for 12.5h; let it stand at room temperature for 2h; then place it in a constant temperature water bath and heat it from 9℃ to 92℃ for 1.5h; finally, immediately place the material at 2.5℃ for rapid cooling for 27.5min; Add the eggshell membrane powder to deionized water at a material-to-liquid ratio of 1:21.5 (g:mL), adjust the pH value to 5.5, and add... Cellulase (0.3% of the eggshell membrane powder mass, 10000 U / g activity) and neutral protease (0.4% of the eggshell membrane powder mass, 15000 U / g activity) were added and the mixture was stirred at 47.5℃ for 3 hours for enzymatic hydrolysis. During the enzymatic hydrolysis, intermittent ultrasonic assistance was initiated at a power of 150W, with ultrasonic treatment lasting 5 minutes every 10 minutes. After the ultrasonic extract was allowed to stand, it was centrifuged and filtered, and the supernatant was collected. The supernatant was then transferred to a rotary evaporator and concentrated under reduced pressure to obtain the eggshell membrane extract. The reduced pressure conditions were set as follows: vacuum degree 0.07 MPa and temperature 42.5℃.

[0022] Diethoxyethyl succinate 2.0%; Menthol lactate 0.4%; Rhamnose lipolipase 1.1%; Sodium subtilis lipopeptide 0.6%; Raspberry ketone glucoside 2.0%; Hexylresorcinol 0.1%; Tranexamic acid 3.0%; Cetearyl alcohol oleate 1.0%; Sorbitan oleate 2.0%; Thickener 0.2%: Hydrolyzed Sclerotium RolfssII gel; Bisabolol 0.5%; Co-surfactant 3.0%: Propylene glycol; Nicotinamide 5.0%; Polydimethylsiloxane 1.0%; The fruit oil content of the avocado tree (BUTYROSPERMUM PARKII) is 0.5%. Hydrogenated polydecene 1.0%; Preservative 1.5%: 1,2-hexanediol; Excipient 0.1%: Poloxamer 407; Deionized water: Balance.

[0023] (2) Pretreatment of the oil phase: Diethoxyethyl succinate, menthol lactate, hexylresorcinol, cetearyl oleate, sorbitan oleate, bisabolol, polydimethylsiloxane, shea butter, and hydrogenated polydecene are mixed and heated to 70°C and stirred until dissolved. Preparation of the aqueous phase: Deionized water is heated to 65°C and then raspberry ketone glucoside, tranexamic acid, hydrolyzed sclerotium gum, eggshell membrane extract, propylene glycol, nicotinamide, and poloxamer 407 are added and stirred until dissolved. The oil phase is slowly added to the aqueous phase, homogenized, and then 1,2-hexanediol, rhamnolipid, and subtilis lipopeptide sodium are added and stirred. The mixture is cooled to room temperature and the pH is adjusted to 5.8 with a citric acid / sodium citrate buffer. After vacuum degassing, the skin care composition is obtained.

[0024] Example 2: This example provides a skincare composition containing whitening and penetration-enhancing ingredients, prepared by: (1) Weigh the raw materials by mass percentage: Eggshell membrane extract 3.5%; Preparation method: Weigh eggshell membrane, clean and dry to constant weight, pulverize and sieve through a 20-mesh sieve to obtain eggshell membrane powder; Freeze eggshell membrane powder at -10℃ for 10h; let stand at room temperature for 1.5h; then place in a constant temperature water bath and heat from 8℃ to 90℃ for 1h; finally, immediately place the material at 0℃ for rapid cooling for 25min; add eggshell membrane powder to deionized water at a material-to-liquid ratio of 1:20 (g:mL), adjust the pH to 4.5, and add fiber. Enzyme (0.5% of the eggshell membrane powder mass, 10000 U / g activity) and neutral protease (0.6% of the eggshell membrane powder mass, 15000 U / g activity) were added and hydrolyzed at 40℃ for 2 hours with stirring. During the hydrolysis, intermittent ultrasonic assistance was initiated at a power of 100W, with ultrasonic treatment lasting 5 minutes every 10 minutes. After the ultrasonic extract was allowed to stand, it was centrifuged and filtered, and the supernatant was collected. The supernatant was then transferred to a rotary evaporator and concentrated under reduced pressure to obtain the eggshell membrane extract. The reduced pressure conditions were set as follows: vacuum degree 0.06 MPa and temperature 40℃.

[0025] Diethoxyethyl succinate 1.3%; Menthol lactate 0.6%; Rhamnose lipolipase 0.5%; Sodium subtilis lipopeptide 1.0%; Raspberry ketone glucoside 0.5%; Hexylresorcinol 0.5%; Tranexamic acid 1.0%; Cetearyl alcohol oleate 3.0%; Sorbitan oleic acid ester 0.5%; Thickener 1.0%: Hydrolyzed Sclerotium RolfssII gum; Bisabolol 0.1%; Co-surfactant 4.2%: Propylene glycol; Nicotinamide 2.0%; Polydimethylsiloxane 3.0%; Avocado (Butyrospermum Parkii) fruit oil content: 2.0%; Hydrogenated polydecene 3.0%; Preservative 0.5%: 1,2-hexanediol; 0.5% excipient: Poloxamer 407; Deionized water: Balance.

[0026] (2) Pretreatment of the oil phase: Diethoxyethyl succinate, menthol lactate, hexylresorcinol, cetearyl oleate, sorbitan oleate, bisabolol, polydimethylsiloxane, shea butter, and hydrogenated polydecene were mixed and heated to 75°C and stirred until dissolved. Preparation of the aqueous phase: Deionized water was heated to 60°C and then raspberry ketone glucoside, tranexamic acid, hydrolyzed sclerotium gum, eggshell membrane extract, propylene glycol, nicotinamide, and poloxamer 407 were added and stirred until dissolved. The oil phase was slowly added to the aqueous phase, homogenized, and then 1,2-hexanediol, rhamnolipid, and sodium subtilis lipopeptide were added and stirred. The mixture was cooled to room temperature and the pH was adjusted to 6.2 with a citric acid / sodium citrate buffer. After vacuum degassing, the skin care composition was obtained.

[0027] Example 3: This example provides a skincare composition containing whitening and penetration-enhancing ingredients, prepared by: (1) Weigh the raw materials by mass percentage: Eggshell membrane extract 2.4%; Preparation method: Weigh eggshell membranes, clean them, dry them to constant weight, pulverize them, and sieve them through a 50-mesh sieve to obtain eggshell membrane powder; Freeze the eggshell membrane powder at -2℃ for 15 hours; let it stand at room temperature for 2.5 hours; then place it in a constant temperature water bath and heat it from 10℃ to 95℃ for 2 hours; finally, immediately place the material at 5℃ for rapid cooling for 30 minutes; add the eggshell membrane powder to deionized water at a material-to-liquid ratio of 1:23 (g:mL), adjust the pH value to 6.5, and add cellulose. Enzymes (0.35% of the eggshell membrane powder mass, enzyme activity 10000 U / g) and neutral protease (0.45% of the eggshell membrane powder mass, enzyme activity 15000 U / g) were added and hydrolyzed at 55℃ for 4 hours with stirring. During the hydrolysis process, intermittent ultrasonic assistance was initiated at a power of 200W, with ultrasonic treatment lasting 5 minutes every 10 minutes. After the ultrasonic extract was allowed to stand, it was centrifuged and filtered, and the supernatant was collected. The supernatant was then transferred to a rotary evaporator and concentrated under reduced pressure to obtain the eggshell membrane extract. The reduced pressure conditions were set as follows: vacuum degree 0.08 MPa and temperature 45℃.

[0028] Diethoxyethyl succinate 1.5%; Menthol lactate 0.5%; Rhamnose lipolipase 0.8%; Sodium subtilis lipopeptide 0.8%; Raspberry ketone glucoside 1.0%; Hexylresorcinol 0.3%; Tranexamic acid 2.0%; Cetearyl alcohol oleate 2.0%; Sorbitan oleate 1.0%; Thickener 0.5%: Hydrolyzed Sclerotium RolfssII gum; Bisabolol 0.4%; Co-surfactant 3.5%: Propylene glycol; Nicotinamide 3.0%; Polydimethylsiloxane 2.0%; Avocado (Butyrospermum Parkii) fruit oil content: 1.0%; Hydrogenated polydecene 2.0%; Preservative 1.0%: 1,2-hexanediol; Excipient 0.3%: Poloxamer 407; Deionized water: Balance.

[0029] (2) Pretreatment of the oil phase: Diethoxyethyl succinate, menthol lactate, hexylresorcinol, cetearyl oleate, sorbitan oleate, bisabolol, polydimethylsiloxane, shea butter, and hydrogenated polydecene are mixed and heated to 72°C and stirred until dissolved. Preparation of the aqueous phase: Deionized water is heated to 65°C and then raspberry ketone glucoside, tranexamic acid, hydrolyzed sclerotium gum, eggshell membrane extract, propylene glycol, nicotinamide, and poloxamer 407 are added and stirred until dissolved. The oil phase is slowly added to the aqueous phase, homogenized, and then 1,2-hexanediol, rhamnolipid, and sodium subtilis lipopeptide are added and stirred. The mixture is cooled to room temperature and the pH is adjusted to 6.0 with a citric acid / sodium citrate buffer. After vacuum degassing, the skin care composition is obtained.

[0030] Comparative Example 1: Unlike Example 1, the ratio of menthol lactate and diethoxyethyl succinate in the raw materials is different: menthol lactate: 0.1% and diethoxyethyl succinate: 2.2%. The remaining raw material components, mass percentages, and preparation methods are consistent with those in Example 1.

[0031] Comparative Example 2: Unlike Example 1, the eggshell membrane extract was removed from the raw materials. All other raw material components, mass percentages, and preparation methods remained the same as in Example 1.

[0032] Comparative Example 3: Unlike Example 1, rhamnolipid was removed from the raw materials. All other raw material components, mass percentages, and preparation methods remained the same as in Example 1.

[0033] Experimental Example 1: Verification of the whitening efficacy of the present invention Table 1 Experimental Groups and Experimental Conditions ; See results Figure 1-2 And Table 2.

[0034] Table 2. Results of Whitening Efficacy Experiment ; use t When performing statistical analysis using the -test method, significance compared to group BC is indicated by #. P -Value < 0.05 is represented by #. P -Value < 0.01 is represented by ##; significance compared to the NC group is represented by *. P -value < 0.05 indicates as *. P -value < 0.01 is represented as **.

[0035] The experimental results show that, compared with the BC group, the melanin granules in the NC group were significantly increased, indicating that the stimulation conditions in this test were effective. Compared with the NC group, the melanin granules in the PC group were significantly decreased, indicating that the positive conditions in this test were effective. Compared with the NC group, the melanin granules in the sample group were significantly decreased, with an inhibition rate of 48.83%.

[0036] Experimental Example 2: Test Method for Soothing Efficacy of Zebrafish (T / ZHCA 016-2022 Evaluation of Soothing Efficacy of Cosmetics by Neutrophil Inhibition Rate Method in Juvenile Zebrafish) Zebrafish were randomly selected and placed in 6-well plates, 15 fish per well. A zebrafish skin inflammation model was established by water-soluble administration of SLS. A normal control group, a model control group, and a sample group (Example 3) were set up, with a volume of 3 mL per well. The plates were incubated at 28°C in the dark for 18 hours. Ten zebrafish from each experimental group were randomly selected and photographed under a fluorescence microscope. Advanced image processing software was used to analyze and collect data, analyzing the number of neutrophils (N) in the zebrafish skin. The soothing effect of the sample was calculated according to a formula to determine whether it possessed a soothing effect.

[0037] ; The experimental results are shown in Figure 3 As can be seen, the number of neutrophils in the sample group was significantly reduced compared with that in the model control group, indicating that the sample has a good soothing effect.

[0038] Experiment Example 3: Penetration Testing Transdermal absorption tests were conducted using pig skin, with the 24-hour nicotinamide permeation rate as the test subject. A higher 24-hour nicotinamide permeation rate indicates a better transdermal penetration effect of the corresponding active ingredient.

[0039] The test results are shown in Table 3.

[0040] Table 3. Results of the penetration test ; Table 3 shows that: Comparative Example 1 data indicates that menthol lactate and diethoxyethyl succinate need to be within a specific ratio range to achieve the best synergistic penetration-enhancing effect. Comparative Example 2 data shows that the 24-hour niacinamide permeation rate is lower than that of Example 1. This indicates that the eggshell membrane extract promotes the transdermal absorption of niacinamide. Comparative Example 3 data shows that the 24-hour niacinamide permeation rate is significantly reduced. Rhamnollipolipids, as biosurfactants, are amphiphilic and can effectively reduce the interfacial tension of the stratum corneum, promote the distribution of niacinamide in the skin, and enhance its transdermal absorption.

[0041] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A skincare composition containing whitening and penetration-enhancing ingredients, characterized in that, It comprises the following components: 1.3-2.0% diethoxyethyl succinate, 0.4-0.6% menthol lactate, 0.5-1.1% rhamnolipid, 0.6-1.0% sodium subtilis lipopeptide, 0.5-2.0% raspberry ketone glucoside, 0.1-0.5% hexylresorcinol, 1.0-3.0% tranexamic acid, 1.0-3.0% cetearyl oleate, 0.5-2.0% sorbitan oleate, 0.2-1.0% thickener, 0.1-0.5% bisabolol, 1.6-3.5% eggshell membrane extract, 3.0-4.2% co-surfactant, 2.0-5.0% niacinamide, 2.5-8.0% skin feel modifier, 0.5-1.5% preservative, 0.1-0.5% excipients, and the balance being deionized water.

2. The skincare composition containing whitening and penetration-enhancing ingredients according to claim 1, characterized in that, The thickener is hydrolyzed sclerotium gum; the co-surfactant is propylene glycol; the preservative is 1,2-hexanediol; the excipient is poloxamer 407; the skin feel modifier comprises polydimethylsiloxane, shea butter, and hydrogenated polydecene in a mass percentage ratio of 1.0-3.0:0.5-2.0:1.0-3.

0.

3. The skincare composition containing whitening and penetration-enhancing ingredients according to claim 1, characterized in that, The preparation method of the eggshell membrane extract includes: The eggshell membrane was subjected to cold and heat treatment and ultrasonic-assisted enzymatic hydrolysis in sequence to obtain an extract; The extract was concentrated to obtain the eggshell membrane extract.

4. The skincare composition containing whitening and penetration-enhancing ingredients according to claim 3, characterized in that, The cold and heat treatment includes: freezing at -10 to -2°C, heating to 90 to 95°C and holding at that temperature, followed by cooling; the ultrasound-assisted enzymatic hydrolysis includes: enzymatic hydrolysis under the action of cellulase and neutral protease, and intermittent ultrasound treatment during the enzymatic hydrolysis process.

5. The skincare composition containing whitening and penetration-enhancing ingredients according to claim 4, characterized in that, The preparation method of eggshell membrane extract includes the following specific steps: (1) Raw material pretreatment Weigh the eggshell membrane, clean it, dry it to constant weight, crush it, and sieve it through a 20-50 mesh to obtain eggshell membrane powder. (2) Cold and heat treatment Freeze the eggshell membrane powder at -10 to -2℃ for 10-15 hours; let it stand at room temperature for 1.5-2.5 hours; then place it in a constant temperature water bath and heat it to 90-95℃ at 8-10℃ for 1-2 hours; finally, immediately place the material at 0-5℃ for rapid cooling for 25-30 minutes. (3) Ultrasound-assisted enzymatic hydrolysis Add eggshell membrane powder to deionized water at a material-to-liquid ratio of 1:20-23, adjust the pH to 4.5-6.5, add cellulase and neutral protease, and stir at 40-55℃ for 2-4 hours for enzymatic hydrolysis; during the enzymatic hydrolysis process, start intermittent ultrasound assistance, with an ultrasound power of 100-200W, and perform ultrasound treatment for 5 minutes every 10 minutes. (4) Filtration and rotary evaporation concentration After the ultrasonic extract was allowed to stand, it was centrifuged and filtered to collect the supernatant. The supernatant was then transferred to a rotary evaporator and concentrated under reduced pressure to obtain the eggshell membrane extract.

6. A method for preparing a skincare composition according to any one of claims 1 to 5, characterized in that, Includes the following steps: Pretreatment of the oil phase: Diethoxyethyl succinate, menthol lactate, hexyl resorcinol, cetearyl oleate, sorbitan oleate, bisabolol, polydimethylsiloxane, shea butter, and hydrogenated polydecene are mixed, heated to 70-75℃, and stirred until dissolved and homogeneous. Preparation of aqueous phase: After heating deionized water to 60-65℃, add raspberry ketone glucoside, tranexamic acid, hydrolyzed sclerotium gum, eggshell membrane extract, propylene glycol, nicotinamide and poloxamer 407, and stir to dissolve; Slowly add the oil phase to the aqueous phase, homogenize, then add 1,2-hexanediol, rhamnolipid, and sodium subtilisin, and continue stirring. Cool to room temperature and adjust the pH to 5.8-6.2 using a citric acid / sodium citrate buffer pair; The skincare composition is obtained after vacuum degassing.

7. The use of a skin care composition according to any one of claims 1 to 5 in the preparation of a whitening skin care product.

8. An application according to claim 7, characterized in that, The amount of the skincare composition added to the whitening skincare product is 15.3-28.6% by weight.

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