A plant preparation for hair and a method for preparing the same

Abstract

The present disclosure relates to fermentation engineering, and provides a plant preparation for hair and a preparation method thereof, which comprises the following steps: washing and crushing traditional Chinese medicine materials to obtain traditional Chinese medicine powder, then mixing the traditional Chinese medicine powder with enzymes and water, and subjecting to enzymolysis to obtain enzymolysis materials, adding yeast to the enzymolysis materials for fermentation to obtain fermentation materials; adding sapogenin to the fermentation materials, and subjecting to incubation and stirring to obtain emulsified materials, then adding sugar acid polysaccharide to the emulsified materials, and subjecting to heating and boiling, and filtration to obtain boiling liquid; concentrating the boiling liquid to obtain a paste, and sequentially adding organic acid salt and acid-releasing agent during the concentration process, and continuing to heat, concentrate, dry, and fill to obtain the plant preparation. The plant preparation prepared by the above method can effectively avoid the loss of volatile oil, protein and polysaccharide in the medicinal materials during the boiling process of various traditional Chinese medicine materials.

Description

Technical Field

[0001] This application relates to the field of fermentation engineering, specifically to a plant-based preparation for hair and its preparation method. Background Technology

[0002] This herbal preparation is made through a process of mixing, decocting, and concentrating multiple medicinal herbs. It works by nourishing blood and essence, strengthening the spleen and removing dampness, and promoting blood circulation. The blood-nourishing components provide the collagen, amino acids, and melanin synthesis raw materials (such as tyrosine, iron, and copper) needed for hair growth, which are then transported to the hair follicles via blood circulation, promoting melanin production and hair shaft repair. The spleen-strengthening and dampness-removing components enhance the spleen and stomach's digestive function, improving the intestines' absorption of nutrients while reducing internal dampness and preventing hair follicle blockage or nutrient supply obstruction due to damp heat accumulation. The blood-activating and nourishing components improve overall microcirculation, allowing blood and essence to nourish the head and face, ensuring oxygen supply to the hair follicles and directly replenishing the lipids needed for hair shaft growth, increasing shine and resilience. The qi-regulating and stomach-soothing components unblock the middle jiao (middle burner) qi mechanism, preventing the nourishing herbs from being too rich and cloying, ensuring that the herbs are nourishing without causing stagnation and are fully absorbed.

[0003] However, in existing decoction techniques, due to the different properties of various medicinal materials and their simultaneous high-temperature treatment, a large amount of volatile active ingredients contained in the medicinal materials are lost during co-distillation in the long-term heating process, which reduces the ability of the ointment to regulate scalp vascular permeability and tyrosinase activity. Furthermore, due to the differences in acid-base properties between different medicinal materials, precipitation reactions occur during co-decoction, causing some protein and polysaccharide effective components to coagulate and precipitate, resulting in a decrease in the content of effective components in the plant preparation obtained after decoction, filtration, and concentration, thereby weakening the final efficacy of the plant preparation. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a plant preparation for hair and a method for preparing the same, in order to solve the problem that the effect of plant preparations is reduced due to the differences in properties between various components during the decoction process.

[0005] To address the aforementioned technical problems, a method for preparing a plant-based preparation for hair is proposed, the method comprising the following steps: S1. After cleaning and crushing the Chinese herbal materials, Chinese herbal powder is obtained. Then, the Chinese herbal powder, compound enzyme and water are mixed and enzymatically hydrolyzed to obtain enzymatically hydrolyzed material. Yeast is then added to the enzymatically hydrolyzed material for fermentation to obtain fermented material. The compound enzyme is composed of a mixture of phosphoester hydrolase and hemicellulase. S2. Add saponins to the fermentation material, keep warm and stir to obtain emulsified material, then add uronic acid polysaccharide to the emulsified material, heat and boil, filter to obtain boiling liquid; S3. Concentrate the decoction to obtain a paste, and add organic acid salts and acid-releasing agents in sequence during the paste-forming process. Continue heating and concentrating, then drying and filling the paste to obtain the plant preparation.

[0006] In addition, a plant preparation for hair is proposed, which is prepared by the above-described method for preparing a plant preparation for hair.

[0007] As can be seen from the above technical solutions, the exemplary embodiments disclosed herein possess at least the following advantages and positive effects: On the one hand, by mixing Chinese herbal medicine powder with compound enzymes and then adding yeast for fermentation, the problems of phytic acid forming insoluble complex precipitates with calcium and magnesium ions and proteins in traditional processes, as well as the retention of hemicellulose entangled with proteins and the oxidation and escape of volatile oils due to lack of pre-protection, can be solved. This achieves efficient release of proteins and polysaccharides, full exposure of the hydrophobic regions of proteins, and preliminary emulsification and locking of volatile oils. Specifically, phosphodiesterase hydrolyzes phytic acid and phosphorylated polysaccharides and phosphorylated proteins, destroying the cell wall skeleton and releasing the trapped effective components, while fully exposing the hydrophobic regions of dephosphorylated proteins; hemicellulase hydrolyzes hemicellulose such as mannan, breaking the physical network of entangled proteins; and mannoproteins produced by yeast fermentation simultaneously emulsify residual volatile oils and consume oxygen to create a reducing environment to protect the volatile oils.

[0008] On the other hand, by adding saponins to the fermentation material, keeping it warm and stirring, and then adding uronic acid polysaccharides for heating and cooking, the problems of protein aggregation and precipitation at high temperatures, free phosphate ions reacting with calcium ions to form calcium phosphate precipitates, and volatile oil loss due to insufficient emulsion film strength can be solved. This achieves stable protein dispersion, effective consumption of phosphate ions, and heat-resistant emulsification protection of volatile oils. Specifically, the hydrophobic aglycones of saponins are embedded in volatile oil microdroplets to form a heat-resistant emulsion film. The hydrophilic sugar chains complement and adsorb the exposed hydrophobic regions of the protein, preventing protein aggregation and anchoring saponins to the protein surface. At the same time, saponins esterify with free phosphate ions to form phosphate saponin esters to consume phosphate ions. The uronic acid polysaccharides encapsulate the protein through hydrogen bonds and electrostatic interactions, forming a polysaccharide-protein complex and grafting uronic acid groups onto the surface of the complex.

[0009] Then, by concentrating the boiling liquid into a paste and sequentially adding organic acid salts and acid-releasing agents, the problems of active ingredients agglomerating and precipitating due to water reduction during concentration, loss of volatile oil due to loss of emulsifying activity after saponin esterification, and oil-water separation due to emulsion film rupture during paste storage can be solved. This achieves gel network locking of active ingredients, regeneration of saponin emulsifying activity, and uniform stability of the paste during long-term storage. Specifically, the calcium ions of organic acid salts coordinate and crosslink with uronic acid to form a uniformly suspended calcium bridge gel network framework, locking proteins and emulsified volatile oils in the pores. The acid-releasing agent slowly hydrolyzes and deacidifies, displacing saponins in phosphate saponin esters to regenerate them into a free state, restoring emulsifying activity to continuously protect volatile oils. During the drying stage, the calcium bridge network further ages and densifies, and the paste becomes uniform and semi-solid with an oily and glossy surface and no water separation.

[0010] Finally, through a three-step synergistic process, S1 exposes the hydrophobic region of the protein and releases phosphate ions; S2 uses the exposed hydrophobic region to adsorb and encapsulate the protein and consume phosphate ions to generate phosphate saponin esters; and S3 uses calcium cross-linking to solidify the backbone and acid decomposition to release saponins for regeneration, effectively avoiding the loss of volatile oils and proteins during the cooking process. Attached Figure Description

[0011] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this disclosure and, together with the description, serve to explain the principles of this disclosure. It is obvious that the drawings described below are merely some embodiments of this disclosure, and those skilled in the art can obtain other drawings based on these drawings without any inventive effort. In the drawings: Figure 1 This is a schematic diagram of a method for preparing a plant-based preparation for hair in one embodiment; Figure 2 This is a schematic diagram of step S1 in a method for preparing a plant-based preparation for hair in one embodiment; Figure 3 This is a schematic diagram of step S2 in a method for preparing a plant-based preparation for hair in one embodiment; Figure 4 This is a schematic diagram of step S3 in a method for preparing a plant-based preparation for hair in one embodiment. Detailed Implementation

[0012] Exemplary embodiments will now be described more fully with reference to the accompanying drawings. However, these exemplary embodiments can be implemented in many forms and should not be construed as limited to the examples set forth herein; rather, these embodiments are provided so that this disclosure will be more thorough and complete, and will fully convey the concept of the exemplary embodiments to those skilled in the art.

[0013] Furthermore, the described features, structures, or characteristics can be combined in any suitable manner in one or more embodiments. Numerous specific details are provided in the following description to give a thorough understanding of embodiments of this disclosure. However, those skilled in the art will recognize that the technical solutions of this disclosure can be practiced without one or more of the specific details, or other methods, components, apparatuses, steps, etc., can be employed. In other instances, well-known methods, apparatuses, implementations, or operations are not shown or described in detail to avoid obscuring various aspects of this disclosure.

[0014] The flowcharts shown in the accompanying drawings are merely illustrative and do not necessarily include all content and operations / steps, nor do they necessarily have to be performed in the described order. For example, some operations / steps can be broken down, while others can be combined or partially combined; therefore, the actual execution order may change depending on the specific circumstances.

[0015] Please refer to Figure 1 This invention proposes a method for preparing a plant-based preparation for hair, the method comprising the following steps: S1. After cleaning and crushing the Chinese herbal materials, Chinese herbal powder is obtained. Then, the Chinese herbal powder, compound enzyme and water are mixed and enzymatically hydrolyzed to obtain enzymatically hydrolyzed material. Yeast is then added to the enzymatically hydrolyzed material for fermentation to obtain fermented material. The compound enzyme is composed of a mixture of phosphate ester hydrolase and hemicellulase.

[0016] The powdered Chinese medicine is enzymatically hydrolyzed with phosphodiesterase and hemicellulase. Phosphodiesterase hydrolyzes phytic acid in herbs such as coix seed, poria cocos, and yam, effectively preventing phytic acid from forming insoluble phytate precipitates and phytate-protein complexes with calcium and magnesium ions and proteins. At the same time, it hydrolyzes phosphorylated polysaccharides and phosphorylated proteins, destroys the cell wall skeleton, releases trapped proteins and polysaccharides, and fully exposes the hydrophobic regions of dephosphorylated proteins. Hemicellulase hydrolyzes hemicellulose such as mannan, cuts the physical network that wraps around proteins, and releases proteins from their trapped state. The mannoprotein produced by yeast fermentation simultaneously emulsifies residual volatile oils and consumes oxygen to create reducing and environmentally friendly volatile oils.

[0017] Step S1 includes: S1.1 Wash the Chinese medicinal materials with clean water, crush them, and pass them through a 20-40 mesh sieve to obtain Chinese medicinal powder. Crushing the Chinese medicinal materials to 20-40 mesh can significantly increase the contact area between the medicinal materials and the compound enzyme, thereby improving the enzymatic hydrolysis efficiency and the dissolution rate of the active ingredients.

[0018] The Chinese medicinal materials are composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche in a mass ratio of (1~3):(2~5):(1~3):(3~6):(5~12):(1~7):(4~10):(3~8):(2~6):(1~4):(3~8):(4~10):(3~8):(2~4):(1~5).

[0019] S1.2 Mix the Chinese herbal powder, compound enzyme and deionized water, keep warm at 40~50℃ for 1.5~2.5h for enzymatic hydrolysis, cool down to 25~35℃ to obtain enzymatic hydrolysate, wherein the mass ratio of Chinese herbal powder, compound enzyme and deionized water is 100:(0.5~1.5):(80~120).

[0020] The complex enzyme is composed of a mixture of phosphoester hydrolase and hemicellulase in a mass ratio of 1:(1~2). The phosphoester hydrolase includes at least one of 3-phytase, 6-phytase, and inositol hexaphosphatase-2, and the hemicellulase includes at least one of mannanase, mannosidase, xylanase, arabinogalactanase, and galactanase.

[0021] The powdered Chinese medicine and the compound enzyme were mixed at a mass ratio of 100:(0.5~1.5) and incubated at 40~50℃ for 1.5~2.5h for enzymatic hydrolysis. Phosphoester hydrolase selectively hydrolyzed phytic acid and phosphorylated polysaccharides / proteins in the medicinal materials, eliminating the ternary complex precipitation of phytic acid-protein-minerals, while fully exposing the hydrophobic region of the protein. Hemicellulase hydrolyzed hemicellulose such as mannan, breaking the physical network that wrapped the protein and releasing the protein from its trapped state. At the same time, the two worked synergistically at a mass ratio of 1:(1~2), with the amount of hemicellulase slightly higher than that of phosphate ester hydrolase, preferentially destroying the cell wall skeleton and creating better substrate contact conditions for phosphate ester hydrolase. After enzymatic hydrolysis, the temperature was lowered to 25~35℃, which both inactivated the enzyme activity to avoid excessive decomposition and provided a suitable temperature for subsequent yeast fermentation, ensuring that the hydrophobic region of the protein in the enzymatically hydrolyzed material was fully exposed and that free phosphate was released in moderation.

[0022] S1.3 Add yeast to the enzymatically hydrolyzed material and ferment at 25-30℃ for 36-48 hours under sealed conditions. Then cool down to 0-5℃ and let it stand for 10-12 hours to obtain fermented material. The amount of yeast added is 0.1-0.3 wt% of the total mass of the Chinese medicine powder.

[0023] Among them, yeast includes at least one of Saccharomyces cerevisiae, ... and Pasteurella multocida.

[0024] Yeast was added to the enzymatically hydrolyzed material at a dosage of 0.1-0.3 wt%, and fermented in a sealed environment at 25-30°C for 36-48 hours. The yeast metabolized to produce mannoproteins, which simultaneously emulsified the volatile oils released prematurely during enzymatic hydrolysis, forming pre-protected droplets. At the same time, the yeast's aerobic respiration rapidly consumed dissolved oxygen in the system, creating a reducing environment and inhibiting the auto-oxidative degradation of the volatile oils. After fermentation, the temperature was lowered to 0-5°C and allowed to stand for 10-12 hours for maturation. Under low-temperature conditions, enzyme activity was significantly inhibited, preventing excessive decomposition and loss of effective components. Simultaneously, it promoted the full adsorption and equilibrium of mannoproteins and volatile oil droplets, and homogenized the diffusion of intermediate products such as free phosphate groups and exposed hydrophobic regions of proteins in the system.

[0025] S2. Add saponins to the fermentation material, keep warm and stir to obtain emulsified material, then heat the emulsified material to a boil, add uronic acid polysaccharide, continue to boil and filter to obtain boiling liquid.

[0026] Adding saponins allows their hydrophobic aglycones to embed into volatile oil droplets, forming a heat-resistant emulsion film. The hydrophilic sugar chains undergo complementary adsorption with the exposed hydrophobic regions of the protein. This adsorption not only prevents protein aggregation and precipitation but also anchors the saponins to the protein surface, forming a complex emulsion layer. Simultaneously, the saponins undergo esterification with free phosphate to generate phosphate saponin esters, consuming phosphate to prevent it from reacting with calcium ions to form calcium phosphate precipitate. The uronic acid polysaccharide encapsulates the protein surface through hydrogen bonds and electrostatic interactions with the uronic acid groups, forming a polysaccharide-protein complex. The uronic acid groups are then grafted onto the surface of the complex.

[0027] Step S2 includes: S2.1 Add saponins and deionized water to the fermentation material and stir at 50~60℃ for 20~30 minutes to obtain emulsified material. The mass ratio of saponins, deionized water and traditional Chinese medicine powder is (1.5~2.5):(400~800):100.

[0028] Among them, saponins include at least one of saponin acid, oleanolic acid, ursolic acid, glycyrrhetinic acid, and ivy saponin.

[0029] Saponins and deionized water were added to the fermentation material at a mass ratio of (1.5~2.5):(400~800):100. The mixture was stirred at 50~60℃ for 20~30 min. Under stirring, the hydrophobic aglycones of the saponins were embedded inside the volatile oil droplets, and the hydrophilic sugar chains extended into the aqueous phase to form a dense emulsion film. This emulsion film had stronger interfacial activity and higher heat resistance than the pre-protective film formed by mannoprotein in S1. At the same time, the mild temperature of 50~60℃ promoted the full dispersion and interfacial arrangement of saponins without causing premature loss of volatile oil. The saponins reacted with the free phosphate in the fermentation material to form phosphate saponin ester, consuming the free phosphate to prevent it from reacting with calcium ions to form calcium phosphate precipitate in the future.

[0030] S2.2 Add uronic acid polysaccharide to the emulsified material, heat to 80~90℃ and boil for 0.8~1.2h, filter to obtain primary filtrate and filter residue, wherein the amount of uronic acid polysaccharide added is 1.0~1.4wt% of the total mass of the Chinese medicine powder.

[0031] Among them, uronic acid polysaccharides include at least one of tea seed polysaccharide, wolfberry polysaccharide, licorice polysaccharide, tremella polysaccharide, moringa leaf polysaccharide, and ganoderma polysaccharide.

[0032] After adding uronic acid polysaccharides to the emulsion material, the mixture is heated to 80-90℃ and boiled. The uronic acid polysaccharides are pre-adsorbed onto the protein surface before the protein undergoes significant thermal denaturation, forming a polysaccharide-protein complex through hydrogen bonding, electrostatic interactions, and hydrophobic interactions. When the temperature rises to 80-90℃, the polysaccharide molecules adsorbed on the protein surface effectively inhibit the hydrophobic aggregation between protein molecules through steric hindrance, preventing the formation of large irreversible precipitates, thereby significantly improving the filtrate permeability and retention rate of the protein during filtration. At the same time, the polysaccharide-protein complex uniformly exposes uronic acid groups on the complex surface, providing sufficient coordination and cross-linking sites for calcium ions of organic acid salts in S3.

[0033] S2.3. Mix the filter residue with deionized water and continue to boil for 20-30 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate with the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:(2-3).

[0034] After mixing the filter residue with deionized water at a ratio of 1:(2~3), continue boiling for 20~30 minutes. The filter residue after the first boiling still contains some soluble components such as free polysaccharides, small molecule peptides, amino acids, minerals, and trace amounts of incompletely dissolved effective substances that are adsorbed onto cell wall fragments and fibrous surfaces. The second boiling, by adding fresh deionized water, breaks the solid-liquid distribution balance and promotes the further dissolution of these residual components.

[0035] In one embodiment, step S2.3 further includes: S2.3 Mix the filter residue with deionized water and continue boiling for 20-30 minutes. Filter to obtain the secondary filtrate. Cool the primary filtrate to 30-40℃ and adjust the pH to 4.0-4.5 with citric acid. Then add 0.5-1.0% flocculant by weight of the total Chinese herbal powder. Stir at 300-450 r / min for 2-5 minutes, then stir at 50-100 r / min for 10-15 minutes. Let stand for 30-60 minutes and filter to obtain the pretreated filtrate. Mix the pretreated filtrate with the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:(2-3). The flocculant includes at least one of chitosan, gelatin, and hydroxypropyl chitosan.

[0036] Adding positively charged flocculants such as chitosan, gelatin, or hydroxypropyl chitosan to the primary filtrate allows for the selective capture of negatively charged tannins and protein-tannin complexes through electrostatic adsorption, forming flocculated precipitates. After filtration, the tannin content in the filtrate is significantly reduced, effectively eliminating the astringent taste caused by tannins and the risk of forming calcium tannate precipitates with subsequent calcium ions. Simultaneously, under acidic conditions of pH 4.0–4.5, the target protein carries a positive charge and repels the positively charged flocculant, thus retaining the protein in the filtrate without flocculation removal. After mixing the primary filtrate (with tannins removed) with the secondary filtrate, the tannin content in the boiling liquid is significantly reduced, while the effective components such as proteins and polysaccharides are well preserved. This provides a purer reaction environment for the coordination and cross-linking of organic acid salt calcium ions and uronic acid groups in the subsequent S3 stage, avoiding the competitive binding of tannins to calcium ions and interference with the formation of calcium bridge networks.

[0037] S3. Concentrate the decoction to obtain a paste, and add organic acid salts and acid-releasing agents in sequence during the paste-forming process. Continue heating and concentrating, then drying and filling the paste to obtain the plant preparation.

[0038] The gel network formed by the cross-linking of calcium ions and uronic acid constructs a stable three-dimensional framework in the paste. This framework locks polysaccharides, proteins, and emulsified volatile oils into the pores through physical embedding, preventing aggregation and precipitation due to water reduction during concentration. Subsequently, the acid-releasing agent slowly hydrolyzes to regenerate saponins into a free state, which are then re-embedded at the oil-water interface to repair emulsion film defects that may occur during concentration, continuously protecting the volatile oils until the final drying stage. Finally, during the drying stage, the calcium bridge network undergoes physical aging, transforming the paste from a semi-fluid to a plastic semi-solid with a glossy, oily surface free from cracking and water separation. At the same time, the regenerated saponins maintain interfacial stability, ensuring that the paste does not separate, precipitate, or lose volatile oils during storage.

[0039] Step S3 includes: S3.1. Concentrate the decoction at 60-70℃ to 45-55% of its original volume, add organic acid salts, and stir for 15-25 minutes to obtain a cross-linked liquid. The amount of organic acid salts added is 0.8-1.2% of the total mass of the Chinese herbal powder.

[0040] Among them, organic acid salts include at least one of calcium lactate, calcium gluconate, calcium citrate, calcium tartrate, and calcium malate.

[0041] After concentrating the boiling liquid to 45-55% of its original volume, organic acid salts are added. The mixture is then kept at 60-70℃ and stirred for 15-25 minutes. This pre-concentration stage removes some water and increases the collision frequency between the uronic acid polysaccharide-protein complex and calcium ions. The added organic acid salts release calcium ions, which coordinate and crosslink with the uronic acid groups grafted onto the surface of the polysaccharide-protein complex in S2, forming a uniform calcium bridge network framework. This transforms the originally dispersed soluble complex into a suspended gel network structure. The mild temperature of 60-70℃ promotes the diffusion of calcium ions and the rate of crosslinking reaction. The stirring and heat preservation ensures that calcium ions are evenly distributed in the concentrated system, and the crosslinking reaction is thorough and uniform. The effective components in the resulting crosslinked liquid are initially locked within the gel network, laying a stable structural foundation for the subsequent final concentration into a paste.

[0042] S3.2 Continue to concentrate the cross-linking liquid to 20-30% of the original decoction volume, add an acid-releasing agent, keep warm and stir for 10-20 minutes to obtain a concentrated liquid, wherein the amount of acid-releasing agent added is 0.5-1.0 wt% of the total mass of the Chinese herbal powder.

[0043] The acid-releasing agent includes at least one of gluconolactone, γ-valerolactone, ribonolactone, and gulonolactone.

[0044] After further concentrating the crosslinking solution to 20-30% of the original boiling liquid volume, add the acid-releasing agent, keep warm and stir for 10-20 minutes. The acid-releasing agent slowly hydrolyzes and releases organic acids, lowering the local micro-zone pH, and displacing the saponins in the phosphate saponin ester generated in S2, regenerating them into a free state. The phosphate ester form of saponins has lost its surface activity and must be released to restore its emulsifying ability. The regenerated free saponins are re-embedded in the oil-water interface, compensating for the emulsion film defects that may be caused by interfacial compression during the concentration process. At the same time, the slow hydrolysis characteristics of the acid-releasing agent avoid the impact of a sudden drop in pH on the protein structure, keeping the volatile oil in the concentrate in a stable emulsion state and the protein dispersed without precipitation.

[0045] S3.3. Concentrate the concentrated liquid at 70~80℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0046] In addition, a plant preparation for hair is proposed, which is prepared by the above-described method for preparing a plant preparation for hair.

[0047] For example, the present invention provides the following specific embodiments to illustrate the specific preparation method: Example 1: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal powder, compound enzyme and deionized water, keep warm at 45℃ for 2 hours for enzymatic hydrolysis, cool down to 30℃ to obtain enzymatic hydrolysate. The mass ratio of Chinese herbal powder, compound enzyme and deionized water is 100:1:100. The compound enzyme is composed of 3-phytase and mannanase mixed at a mass ratio of 1:1.5. S1.3. Next, add the brewing yeast to the enzymatically hydrolyzed material, ferment at 28℃ for 42 hours under sealed conditions, then cool to 0~5℃ and let it stand for 11 hours to mature, obtaining the fermented material. The amount of brewing yeast added is 0.2wt% of the total mass of the Chinese herbal powder. S2.1 Add saponin acid and deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material. The mass ratio of saponin acid, deionized water and traditional Chinese medicine powder is 2:600:100. S2.2 Add tea seed polysaccharide to the emulsified material, heat to 85℃ and boil for 1 hour, filter to obtain primary filtrate and filter residue, wherein the amount of tea seed polysaccharide added is 1.2 wt% of the total mass of the Chinese herbal powder; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate and the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the decoction to 50% of its original volume at 65℃, add calcium lactate, and stir for 20 minutes to obtain a cross-linked solution. The amount of calcium lactate added is 1% of the total mass of the Chinese herbal powder. S3.2. Continue to concentrate the cross-linking solution to 25% of the original decoction volume, add glucono-delta-lactone, keep warm and stir for 15 minutes to obtain a concentrated solution, wherein the amount of glucono-delta-lactone added is 0.8 wt% of the total mass of the Chinese herbal powder; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0048] Example 2: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal powder, compound enzyme and deionized water, keep warm at 45℃ for 2 hours for enzymatic hydrolysis, cool down to 30℃ to obtain enzymatic hydrolysate. The mass ratio of Chinese herbal powder, compound enzyme and deionized water is 100:1:100. The compound enzyme is composed of 3-phytase and mannanase mixed at a mass ratio of 1:1.5. S1.3. Next, add the brewing yeast to the enzymatically hydrolyzed material, ferment at 28℃ for 42 hours under sealed conditions, then cool to 0~5℃ and let it stand for 11 hours to mature, obtaining the fermented material. The amount of brewing yeast added is 0.2wt% of the total mass of the Chinese herbal powder. S2.1 Add saponin acid and deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material. The mass ratio of saponin acid, deionized water and traditional Chinese medicine powder is 2:600:100. S2.2 Add tea seed polysaccharide to the emulsified material, heat to 85℃ and boil for 1 hour, filter to obtain primary filtrate and filter residue, wherein the amount of tea seed polysaccharide added is 1.2 wt% of the total mass of the Chinese herbal powder; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Cool the primary filtrate to 35°C and adjust the pH to 4.0~4.5 with citric acid. Then add 0.8% chitosan by weight of the total Chinese medicine powder, stir at 380 r / min for 3 minutes, then stir at 80 r / min for 12 minutes, let stand for 45 minutes, filter to obtain the pretreated filtrate, and then mix the pretreated filtrate with the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the decoction to 50% of its original volume at 65℃, add calcium lactate, and stir for 20 minutes to obtain a cross-linked solution. The amount of calcium lactate added is 1% of the total mass of the Chinese herbal powder. S3.2. Continue to concentrate the cross-linking solution to 25% of the original decoction volume, add glucono-delta-lactone, keep warm and stir for 15 minutes to obtain a concentrated solution, wherein the amount of glucono-delta-lactone added is 0.8 wt% of the total mass of the Chinese herbal powder; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0049] Comparative Example 1: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal powder, 3-phytase and deionized water, keep warm at 45℃ for 2 hours for enzymatic hydrolysis, and cool down to 30℃ to obtain the enzymatic hydrolysate. The mass ratio of Chinese herbal powder, 3-phytase and deionized water is 100:1:100. S1.3. Next, add the brewing yeast to the enzymatically hydrolyzed material, ferment at 28℃ for 42 hours under sealed conditions, then cool to 0~5℃ and let it stand for 11 hours to mature, obtaining the fermented material. The amount of brewing yeast added is 0.2wt% of the total mass of the Chinese herbal powder. S2.1 Add saponin acid and deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material. The mass ratio of saponin acid, deionized water and traditional Chinese medicine powder is 2:600:100. S2.2 Add tea seed polysaccharide to the emulsified material, heat to 85℃ and boil for 1 hour, filter to obtain primary filtrate and filter residue, wherein the amount of tea seed polysaccharide added is 1.2 wt% of the total mass of the Chinese herbal powder; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate and the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the decoction to 50% of its original volume at 65℃, add calcium lactate, and stir for 20 minutes to obtain a cross-linked solution. The amount of calcium lactate added is 1% of the total mass of the Chinese herbal powder. S3.2. Continue to concentrate the cross-linking solution to 25% of the original decoction volume, add glucono-delta-lactone, keep warm and stir for 15 minutes to obtain a concentrated solution, wherein the amount of glucono-delta-lactone added is 0.8 wt% of the total mass of the Chinese herbal powder; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0050] The difference from Example 1 is that only 3-phytase was added in step S1.2, and no mannanase was added.

[0051] Comparative Example 2: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal powder, mannanase and deionized water, keep warm at 45℃ for 2 hours for enzymatic hydrolysis, and cool down to 30℃ to obtain the enzymatic hydrolysate. The mass ratio of Chinese herbal powder, mannanase and deionized water is 100:1:100. S1.3. Next, add the brewing yeast to the enzymatically hydrolyzed material, ferment at 28℃ for 42 hours under sealed conditions, then cool to 0~5℃ and let it stand for 11 hours to mature, obtaining the fermented material. The amount of brewing yeast added is 0.2wt% of the total mass of the Chinese herbal powder. S2.1 Add saponin acid and deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material. The mass ratio of saponin acid, deionized water and traditional Chinese medicine powder is 2:600:100. S2.2 Add tea seed polysaccharide to the emulsified material, heat to 85℃ and boil for 1 hour, filter to obtain primary filtrate and filter residue, wherein the amount of tea seed polysaccharide added is 1.2 wt% of the total mass of the Chinese herbal powder; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate and the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the decoction to 50% of its original volume at 65℃, add calcium lactate, and stir for 20 minutes to obtain a cross-linked solution. The amount of calcium lactate added is 1% of the total mass of the Chinese herbal powder. S3.2. Continue to concentrate the cross-linking solution to 25% of the original decoction volume, add glucono-delta-lactone, keep warm and stir for 15 minutes to obtain a concentrated solution, wherein the amount of glucono-delta-lactone added is 0.8 wt% of the total mass of the Chinese herbal powder; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0052] The difference from Example 1 is that only mannanase was added in step S1.2, and 3-phytase was not added.

[0053] Comparative Example 3: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal medicine powder with deionized water, keep it at 45℃ for 2 hours, and then cool it down to 30℃ to obtain the enzymatic hydrolysate. The mass ratio of the Chinese herbal medicine powder to the deionized water is 100:1:100. S1.3. Next, add the brewing yeast to the enzymatically hydrolyzed material, ferment at 28℃ for 42 hours under sealed conditions, then cool to 0~5℃ and let it stand for 11 hours to mature, obtaining the fermented material. The amount of brewing yeast added is 0.2wt% of the total mass of the Chinese herbal powder. S2.1 Add saponin acid and deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material. The mass ratio of saponin acid, deionized water and traditional Chinese medicine powder is 2:600:100. S2.2 Add tea seed polysaccharide to the emulsified material, heat to 85℃ and boil for 1 hour, filter to obtain primary filtrate and filter residue, wherein the amount of tea seed polysaccharide added is 1.2 wt% of the total mass of the Chinese herbal powder; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate and the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the decoction to 50% of its original volume at 65℃, add calcium lactate, and stir for 20 minutes to obtain a cross-linked solution. The amount of calcium lactate added is 1% of the total mass of the Chinese herbal powder. S3.2. Continue to concentrate the cross-linking solution to 25% of the original decoction volume, add glucono-delta-lactone, keep warm and stir for 15 minutes to obtain a concentrated solution, wherein the amount of glucono-delta-lactone added is 0.8 wt% of the total mass of the Chinese herbal powder; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0054] The difference from Example 1 is that 3-phytase and mannanase were not added in step S1.2, that is, the complex enzyme was not used in step S1.

[0055] Comparative Example 4: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal powder, compound enzyme and deionized water, keep warm at 45℃ for 2 hours for enzymatic hydrolysis, cool down to 30℃ to obtain enzymatic hydrolysate. The mass ratio of Chinese herbal powder, compound enzyme and deionized water is 100:1:100. The compound enzyme is composed of 3-phytase and mannanase mixed at a mass ratio of 1:1.5. S1.3. Then, the enzymatically hydrolyzed material is first allowed to stand and mature at 28°C for 42 hours under sealed conditions, and then cooled to 0~5°C and allowed to stand and mature for 11 hours to obtain the fermented material. S2.1 Add saponin acid and deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material. The mass ratio of saponin acid, deionized water and traditional Chinese medicine powder is 2:600:100. S2.2 Add tea seed polysaccharide to the emulsified material, heat to 85℃ and boil for 1 hour, filter to obtain primary filtrate and filter residue, wherein the amount of tea seed polysaccharide added is 1.2 wt% of the total mass of the Chinese herbal powder; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate and the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the decoction to 50% of its original volume at 65℃, add calcium lactate, and stir for 20 minutes to obtain a cross-linked solution. The amount of calcium lactate added is 1% of the total mass of the Chinese herbal powder. S3.2. Continue to concentrate the cross-linking solution to 25% of the original decoction volume, add glucono-delta-lactone, keep warm and stir for 15 minutes to obtain a concentrated solution, wherein the amount of glucono-delta-lactone added is 0.8 wt% of the total mass of the Chinese herbal powder; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0056] The difference from Example 1 is that brewing yeast was not added in step S1.3, that is, yeast was not used in step S1.

[0057] Comparative Example 5: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal powder, compound enzyme and deionized water, keep warm at 45℃ for 2 hours for enzymatic hydrolysis, cool down to 30℃ to obtain enzymatic hydrolysate. The mass ratio of Chinese herbal powder, compound enzyme and deionized water is 100:1:100. The compound enzyme is composed of 3-phytase and mannanase mixed at a mass ratio of 1:1.5. S1.3. Next, add the brewing yeast to the enzymatically hydrolyzed material, ferment at 28℃ for 42 hours under sealed conditions, then cool to 0~5℃ and let it stand for 11 hours to mature, obtaining the fermented material. The amount of brewing yeast added is 0.2wt% of the total mass of the Chinese herbal powder. S2.1 Add deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material, wherein the mass ratio of deionized water to traditional Chinese medicine powder is 600:100. S2.2 Add tea seed polysaccharide to the emulsified material, heat to 85℃ and boil for 1 hour, filter to obtain primary filtrate and filter residue, wherein the amount of tea seed polysaccharide added is 1.2 wt% of the total mass of the Chinese herbal powder; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate and the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the decoction to 50% of its original volume at 65℃, add calcium lactate, and stir for 20 minutes to obtain a cross-linked solution. The amount of calcium lactate added is 1% of the total mass of the Chinese herbal powder. S3.2. Continue to concentrate the cross-linking solution to 25% of the original decoction volume, add glucono-delta-lactone, keep warm and stir for 15 minutes to obtain a concentrated solution, wherein the amount of glucono-delta-lactone added is 0.8 wt% of the total mass of the Chinese herbal powder; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0058] The difference from Example 1 is that saponin acid was not added in step S2.1, that is, saponinogen was not added in step S2.

[0059] Comparative Example 6: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal powder, compound enzyme and deionized water, keep warm at 45℃ for 2 hours for enzymatic hydrolysis, cool down to 30℃ to obtain enzymatic hydrolysate. The mass ratio of Chinese herbal powder, compound enzyme and deionized water is 100:1:100. The compound enzyme is composed of 3-phytase and mannanase mixed at a mass ratio of 1:1.5. S1.3. Next, add the brewing yeast to the enzymatically hydrolyzed material, ferment at 28℃ for 42 hours under sealed conditions, then cool to 0~5℃ and let it stand for 11 hours to mature, obtaining the fermented material. The amount of brewing yeast added is 0.2wt% of the total mass of the Chinese herbal powder. S2.1 Add saponin acid and deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material. The mass ratio of saponin acid, deionized water and traditional Chinese medicine powder is 2:600:100. S2.2 Heat the emulsified material to 85°C and boil for 1 hour, then filter to obtain primary filtrate and filter residue; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate and the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the decoction to 50% of its original volume at 65℃, add calcium lactate, and stir for 20 minutes to obtain a cross-linked solution. The amount of calcium lactate added is 1% of the total mass of the Chinese herbal powder. S3.2. Continue to concentrate the cross-linking solution to 25% of the original decoction volume, add glucono-delta-lactone, keep warm and stir for 15 minutes to obtain a concentrated solution, wherein the amount of glucono-delta-lactone added is 0.8 wt% of the total mass of the Chinese herbal powder; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0060] The difference from Example 1 is that tea seed polysaccharide was not added in step S2.2, that is, uronic acid polysaccharide was not used in step S2.

[0061] Comparative Example 7: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal powder, compound enzyme and deionized water, keep warm at 45℃ for 2 hours for enzymatic hydrolysis, cool down to 30℃ to obtain enzymatic hydrolysate. The mass ratio of Chinese herbal powder, compound enzyme and deionized water is 100:1:100. The compound enzyme is composed of 3-phytase and mannanase mixed at a mass ratio of 1:1.5. S1.3. Next, add the brewing yeast to the enzymatically hydrolyzed material, ferment at 28℃ for 42 hours under sealed conditions, then cool to 0~5℃ and let it stand for 11 hours to mature, obtaining the fermented material. The amount of brewing yeast added is 0.2wt% of the total mass of the Chinese herbal powder. S2.1 Add saponin acid and deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material. The mass ratio of saponin acid, deionized water and traditional Chinese medicine powder is 2:600:100. S2.2 Add tea seed polysaccharide to the emulsified material, heat to 85℃ and boil for 1 hour, filter to obtain primary filtrate and filter residue, wherein the amount of tea seed polysaccharide added is 1.2 wt% of the total mass of the Chinese herbal powder; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate and the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the cooking liquid at 65℃ to 50% of its original volume, and stir for 20 minutes to obtain the crosslinking liquid; S3.2. Continue to concentrate the cross-linking solution to 25% of the original decoction volume, add glucono-delta-lactone, keep warm and stir for 15 minutes to obtain a concentrated solution, wherein the amount of glucono-delta-lactone added is 0.8 wt% of the total mass of the Chinese herbal powder; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0062] The difference from Example 1 is that calcium lactate was not added in step S3.1, that is, no organic acid salts were used in step S3.

[0063] Comparative Example 8: S1.1 The Chinese medicinal materials composed of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3 are washed with clean water, crushed, and passed through a 30-mesh sieve to obtain Chinese medicinal powder. S1.2 Mix the Chinese herbal powder, compound enzyme and deionized water, keep warm at 45℃ for 2 hours for enzymatic hydrolysis, cool down to 30℃ to obtain enzymatic hydrolysate. The mass ratio of Chinese herbal powder, compound enzyme and deionized water is 100:1:100. The compound enzyme is composed of 3-phytase and mannanase mixed at a mass ratio of 1:1.5. S1.3. Next, add the brewing yeast to the enzymatically hydrolyzed material, ferment at 28℃ for 42 hours under sealed conditions, then cool to 0~5℃ and let it stand for 11 hours to mature, obtaining the fermented material. The amount of brewing yeast added is 0.2wt% of the total mass of the Chinese herbal powder. S2.1 Add saponin acid and deionized water to the fermentation material and stir at 55°C for 25 minutes to obtain emulsified material. The mass ratio of saponin acid, deionized water and traditional Chinese medicine powder is 2:600:100. S2.2 Add tea seed polysaccharide to the emulsified material, heat to 85℃ and boil for 1 hour, filter to obtain primary filtrate and filter residue, wherein the amount of tea seed polysaccharide added is 1.2 wt% of the total mass of the Chinese herbal powder; S2.3. Mix the filter residue with deionized water and continue to boil for 25 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate and the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:2.5. S3.1. Concentrate the decoction to 50% of its original volume at 65℃, add calcium lactate, and stir for 20 minutes to obtain a cross-linked solution. The amount of calcium lactate added is 1% of the total mass of the Chinese herbal powder. S3.2. Continue to concentrate the crosslinking solution to 25% of the original cooking liquid volume, keep warm and stir for 15 minutes to obtain the concentrated solution; S3.3. Concentrate the concentrate at 75℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

[0064] The difference from Example 1 is that gluconolactone was not added in step S3.2, that is, no acid-releasing agent was used in step S3.

[0065] Comparative Example 9: The specific preparation method of plant preparations is as follows: The Chinese medicinal materials, consisting of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola in a mass ratio of 2:3.5:2:4:8:4:7:5:4:2.5:5:7:5:3:3, were washed with clean water, crushed, and passed through a 30-mesh sieve to obtain a powder. The powder was then mixed with deionized water in a mass ratio of 100:700, heated to 85°C and simmered for 2 hours. The mixture was filtered to obtain a decoction, which was then concentrated at 75°C to form a paste. Samples were taken every 15 minutes, cooled to 25°C, and measured. When the relative density of the sample reached 1.35~1.40, heating was stopped, the sample was allowed to cool to room temperature, and then bottled to obtain a plant preparation.

[0066] Performance testing: Volatile oil content test: The volatile oil content in the sample was determined by steam distillation. 50g of the plant preparation sample was weighed and placed in a round-bottom flask. 300mL of water and several glass beads were added, and the mixture was shaken and connected to the volatile oil analyzer and reflux condenser. Water was added from the top of the condenser until it filled the graduated section of the volatile oil analyzer and overflowed into the flask. The flask was placed in a heating mantle and slowly heated to boiling, maintaining a gentle boil for 5 hours until the oil volume in the analyzer stopped increasing. Heating was then stopped. After cooling to room temperature, the stopcock at the bottom of the analyzer was opened to slowly release the water. The volume of the oil layer in the graduated tube was read, and the volatile oil content (mL / g) was calculated.

[0067] Total protein content test: The Kjeldahl method was used to determine the total protein content. 2g of the plant preparation sample was weighed and placed in a digestion tube. 0.3g of copper sulfate, 6g of potassium sulfate, and 20mL of concentrated sulfuric acid were added. After shaking well, the sample was placed on a digestion furnace and heated gently until complete carbonization and foaming ceased. Then, the temperature was increased to 420℃ and digestion continued for 2-3 hours until the solution turned a clear blue-green color. The sample was then removed and cooled. The digestion tube was placed on an automated Kjeldahl nitrogen analyzer, and 40% sodium hydroxide solution was added to the reaction tube. The solution was distilled, and the distillate was collected in a 2% boric acid solution (containing a mixed indicator). Titration was performed with 0.05mol / L sulfuric acid titrant until the solution changed from blue-green to gray-purple. The titration volume was recorded, and a blank control was performed simultaneously. The total nitrogen content was calculated and multiplied by the protein conversion factor of 6.25 to obtain the total protein content (mg / g).

[0068] The sample test data are shown in Table 1.

[0069] Table 1. Content of ingredients in plant preparations: According to the data in Table 1, compared with Comparative Example 9, the volatile oil and protein contents of the plant preparations prepared in Examples 1-2 are higher than those in Comparative Example 9, with Example 2 having the highest content. At the same time, the centrifugal sedimentation rate (centrifugation at 3000 r / min for 15 min) and storage stability (accelerated test at 40℃ for 30 days) of the plant preparations prepared in Examples 1-2 were measured. The centrifugal sedimentation rate of both was less than 1%, and there was no stratification or off-odor after the accelerated test. Therefore, both have good stability.

[0070] Further observation of Comparative Examples 1-8 revealed that when only a single enzyme is used during enzymatic hydrolysis, or when no compound enzyme, yeast, saponin, uronic acid polysaccharide, organic acid salt, or acid-releasing agent is added, the content of volatile oil and protein in the plant preparation will decrease.

[0071] Other embodiments of this disclosure will readily occur to those skilled in the art upon consideration of the specification and practice of the invention disclosed herein. This application is intended to cover any variations, uses, or adaptations of this disclosure that follow the general principles of this disclosure and include common knowledge or customary techniques in the art not disclosed herein. The specification and examples are to be considered exemplary only, and the true scope and spirit of this disclosure are indicated by the following claims.

[0072] It should be understood that this disclosure is not limited to the precise structures described above and shown in the accompanying drawings, and various modifications and changes can be made without departing from its scope. The scope of this disclosure is limited only by the appended claims.

Claims

1. A method for preparing a plant-based preparation for hair, characterized in that, The preparation method includes the following steps: S1. After cleaning and crushing the Chinese herbal materials, Chinese herbal powder is obtained. Then, the Chinese herbal powder, compound enzyme and water are mixed and enzymatically hydrolyzed to obtain enzymatically hydrolyzed material. Yeast is then added to the enzymatically hydrolyzed material for fermentation to obtain fermented material. The compound enzyme is composed of a mixture of phosphoester hydrolase and hemicellulase. S2. Add saponins to the fermentation material, keep warm and stir to obtain emulsified material, then add uronic acid polysaccharide to the emulsified material, heat and boil, filter to obtain boiling liquid; S3. Concentrate the decoction to obtain a paste, and add organic acid salts and acid-releasing agents in sequence during the paste-forming process. Continue heating and concentrating, then drying and filling the paste to obtain the plant preparation.

2. The method for preparing a plant-based preparation for hair according to claim 1, characterized in that, Step S1 includes: S1.1 Wash the Chinese medicinal materials with clean water, crush them, and pass them through a 20-40 mesh sieve to obtain Chinese medicinal powder; S1.2 Mix the Chinese herbal powder, compound enzyme and deionized water, keep warm at 40~50℃ for 1.5~2.5h for enzymatic hydrolysis, cool down to 25~35℃ to obtain enzymatic hydrolysate, wherein the mass ratio of Chinese herbal powder, compound enzyme and deionized water is 100:(0.5~1.5):(80~120); S1.3 Add yeast to the enzymatically hydrolyzed material and ferment at 25-30℃ for 36-48 hours under sealed conditions. Then cool down to 0-5℃ and let it stand for 10-12 hours to obtain fermented material. The amount of yeast added is 0.1-0.3 wt% of the total mass of the Chinese medicine powder.

3. The method for preparing a plant-based preparation for hair according to claim 2, characterized in that, The Chinese medicinal materials are composed of a mixture of Xinhui tangerine peel, angelica, angelica dahurica, mulberry, black sesame, peach kernel, donkey-hide gelatin, polygonatum, wolfberry, longan pulp, codonopsis, yam, coix seed, poria cocos, and cistanche deserticola. The mass ratio of phosphodiesterase to hemicellulase in the compound enzyme is 1:(1~2). The phosphodiesterase includes at least one of 3-phytase, 6-phytase, and inositol hexaphosphatase-2. The hemicellulase includes at least one of mannanase, mannosidase, xylanase, arabinogalactanase, and galactanase. The yeast includes at least one of brewer's yeast, basil yeast, and pastort yeast.

4. The method for preparing a plant-based preparation for hair according to claim 3, characterized in that, The mass ratio of the following Chinese medicinal materials is (1~3):(2~5):(1~3):(3~6):(5~12):(1~7):(4~10):(3~8):(2~6):(1~4):(3~8):(4~10):(3~8):(2~4):(1~5).

5. The method for preparing a plant-based preparation for hair according to claim 1, characterized in that, Step S2 includes: S2.1 Add saponins and deionized water to the fermentation material and stir at 50~60℃ for 20~30 min to obtain emulsified material. The mass ratio of saponins, deionized water and traditional Chinese medicine powder is (1.5~2.5):(400~800):

100. S2.2 Add uronic acid polysaccharide to the emulsified material, heat to 80~90℃ and cook for 0.8~1.2h, filter to obtain primary filtrate and filter residue, wherein the amount of uronic acid polysaccharide added is 1.0~1.4wt% of the total mass of the Chinese herbal medicine powder; S2.

3. Mix the filter residue with deionized water and continue to boil for 20-30 minutes. Filter to obtain the secondary filtrate. Mix the primary filtrate with the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:(2-3).

6. A method for preparing a plant-based preparation for hair according to claim 5, characterized in that, Saponins include at least one of saponin acid, oleanolic acid, ursolic acid, glycyrrhetinic acid, and ivy saponin; uronic acid polysaccharides include at least one of tea seed polysaccharide, wolfberry polysaccharide, licorice polysaccharide, tremella polysaccharide, moringa leaf polysaccharide, and ganoderma polysaccharide.

7. A method for preparing a plant-based preparation for hair according to claim 5, characterized in that, Step S2.3 also includes: S2.3 Mix the filter residue with deionized water and continue boiling for 20-30 minutes. Filter to obtain the secondary filtrate. Cool the primary filtrate to 30-40℃ and adjust the pH to 4.0-4.5 with citric acid. Then add 0.5-1.0% flocculant by weight of the total Chinese herbal powder. Stir at 300-450 r / min for 2-5 minutes, then stir at 50-100 r / min for 10-15 minutes. Let stand for 30-60 minutes and filter to obtain the pretreated filtrate. Mix the pretreated filtrate with the secondary filtrate to obtain the boiling liquid. The mass ratio of filter residue to deionized water is 1:(2-3). The flocculant includes at least one of chitosan, gelatin, and hydroxypropyl chitosan.

8. A method for preparing a plant-based preparation for hair according to claim 1, characterized in that, Step S3 includes: S3.

1. Concentrate the decoction to 45-55% of its original volume by keeping it at 60-70℃, add organic acid salts, and stir for 15-25 minutes to obtain a cross-linked liquid. The amount of organic acid salts added is 0.8-1.2% of the total mass of the Chinese herbal powder. S3.

2. Continue to concentrate the cross-linking solution to 20-30% of the original decoction volume, add the acid-releasing agent, keep warm and stir for 10-20 minutes to obtain the concentrated solution. The amount of acid-releasing agent added is 0.5-1.0 wt% of the total mass of the Chinese herbal powder. S3.

3. Concentrate the concentrated liquid at 70~80℃ to obtain an extract. Take a sample every 15 minutes, cool it to 25℃ and measure it. When the relative density of the sample reaches 1.35~1.40, stop heating, let it cool to room temperature, and fill it to obtain the plant preparation.

9. A method for preparing a plant-based preparation for hair according to claim 8, characterized in that, Organic acid salts include at least one of calcium lactate, calcium gluconate, calcium citrate, calcium tartrate, and calcium malate, and acid-releasing agents include at least one of gluconolactone, γ-valerolactone, ribonucleolactone, and gulonucleolactone.

10. A plant-based preparation for hair, characterized in that, The plant preparation is obtained by the preparation method of a plant preparation for hair as described in any one of claims 1-9.

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