A traditional Chinese medicine formula emulsion for treating multiple infections of animal ears, a preparation method and application thereof

Abstract

The application discloses a traditional Chinese medicine composition emulsion for treating multiple infections of animal ears, a preparation method and application thereof; the traditional Chinese medicine composition for treating multiple infections of ears comprises the following components in mass fractions: gallnut 28-32 parts, Coptis chinensis 13-17 parts, Radix et Rhizoma Rhei 13-17 parts, Sophora flavescens 28-32 parts, Phellodendri Cortex 8-12 parts, Cnidium 28-32 parts, salt cake 8-12 parts, Zanthoxylum 8-12 parts, Atractylodes 8-12 parts, borneol 3-7 parts and tea tree oil 8-12 parts. The traditional Chinese medicine composition has the effects of clearing heat and drying dampness, killing insects and stopping itching, absorbing dampness and healing sores, and removing swelling and promoting tissue regeneration, and has good treatment effects on bacterial, fungal or mite infections of ears.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine (veterinary medicine) technology, and relates to a traditional Chinese medicine emulsion for treating multiple ear infections in animals, its preparation method and application. Background Technology

[0002] Animal ear infections are mostly caused by pathogens such as bacteria, fungi, or mites, which can lead to itching, pain, redness, swelling, or purulent discharge in the ear canal, accompanied by an odor; in severe cases, it can cause otitis media, resulting in hearing loss or head and neck tilt.

[0003] Commonly used treatments are selected based on the specific pathogen, including organochlorine, organophosphate, pyrethroid, formamid, and macrolide insecticides for mites; various antibiotics for bacteria; and antifungal drugs for fungi. However, due to long-term, excessive, and even indiscriminate use of these drugs, almost all widely used anti-mite, antibacterial, and antifungal drugs have resulted in the emergence of drug-resistant insect or bacterial strains, leading to increasingly serious trends of multidrug resistance and cross-resistance. The development of new drugs often lags behind the rate of resistance development, thus increasing the need for novel, low-residue, safe, and reliable alternative drugs. Summary of the Invention

[0004] Purpose of the invention: The purpose of this invention is to provide a traditional Chinese medicine emulsion for treating multiple ear infections in animals, which aims to offer a new treatment option. Another object of the present invention is to provide a method for preparing the emulsion of the traditional Chinese medicine formula.

[0005] A third objective of this invention is to provide specific applications of the traditional Chinese medicine formula emulsion.

[0006] Technical solution: The present invention provides a traditional Chinese medicine emulsion for treating multiple ear infections in animals, characterized in that the traditional Chinese medicine composition consists of traditional Chinese medicine composition A and traditional Chinese medicine composition B; The composition of the traditional Chinese medicine A is as follows: 28-32 parts of Gallnut, 13-17 parts of Coptis chinensis, 13-17 parts of Rheum palmatum, 28-32 parts of Sophora flavescens, 8-12 parts of Phellodendron chinense, 28-32 parts of Cnidium monnieri, 8-12 parts of Alum, 8-12 parts of Zanthoxylum bungeanum, 8-12 parts of Atractylodes macrocephala, and 3-7 parts of Borneol. The composition of the traditional Chinese medicine composition B is as follows: 8-12 parts of tea tree oil.

[0007] Furthermore, the composition of the traditional Chinese medicine is as follows: 30 parts of Gallnut, 15 parts of Coptis chinensis, 15 parts of Rheum palmatum, 30 parts of Sophora flavescens, 10 parts of Phellodendron chinense, 30 parts of Cnidium monnieri, 10 parts of Alum, 10 parts of Zanthoxylum bungeanum, 10 parts of Atractylodes macrocephala, 5 parts of Borneol and 10 parts of Tea Tree Oil.

[0008] Furthermore, the present invention also provides a method for preparing a traditional Chinese medicine formula emulsion for treating multiple ear infections in animals, comprising the following steps: Step (1) Soak the raw materials to be decocted, excluding tea tree oil, in 3 times their volume of 75% ethanol. After soaking in step (2), the raw material is heated to boiling in a rotary evaporator at a water bath temperature of 55–60 ℃. After boiling, the water bath temperature is reduced to 45–50 ℃ and the decoction is continued. After decoction, the dregs are filtered through an 80-mesh filter and the decoction is collected. The filtered dregs are used for decoction again. Step (3) Add 3 times the volume of 75% ethanol to the filtered residue. Use a rotary evaporator to heat the soaked raw material to boiling in a water bath at a temperature of 55–60 ℃. After boiling, reduce the water bath temperature to 45–50 ℃ and continue decocting. After decocting, filter the residue through an 80-mesh filter and discard it. Collect the decoction. Combine the two decoctions and concentrate them over a low flame to the same volume as the raw material to make the final alcohol extract; that is, a traditional Chinese medicine formula emulsion for treating multiple ear infections in animals. After filtration in step (4), the liquid was freeze-dried and the freeze-dried powder was prepared into an alcohol extract of 30 mg / mL using sterile water. 0.8 g of tea tree oil was accurately weighed, and 0.4 g of Tween 80 and 0.04 g of sodium carboxymethyl cellulose were slowly added to the tea tree oil and stirred (1000 r / min) until completely dissolved. Then, about 27 mL of alcohol extract was slowly added and the mixture was stirred for another 10 min. The mixture was then sonicated at 400 W, 20 kHz and 40% power, frequency and amplitude for 10 min to prepare a 30 mg / mL self-made traditional Chinese medicine emulsion.

[0009] Furthermore, the soaking time described in step (1) is as follows: 1.5 h for leafy and stem drugs, and 3 h for drugs mainly composed of roots and fruits; The time for continued low-temperature simmering in step (2) is 2 hours; The time for continued low-temperature simmering in step (3) is 2 hours.

[0010] Furthermore, the present invention also provides the application of the above-mentioned traditional Chinese medicine formula emulsion for treating multiple ear infections in animals in the preparation of a formula that reduces the bacterial content in multiple ear infections in animals.

[0011] Furthermore, the present invention also provides the application of the above-mentioned traditional Chinese medicine formula emulsion for treating multiple ear infections in animals in the preparation of a formula that reduces the fungal content in multiple ear infections in animals.

[0012] Furthermore, the present invention also provides the application of the above-mentioned traditional Chinese medicine formula emulsion for treating multiple ear infections in animals in the preparation of a formula to reduce the number of itch mites in multiple ear infections in animals; The reduction of the number of itch mites refers to: observing the number of itch mites in the ears of experimental animals daily; .

[0013] Furthermore, the present invention also provides the application of the above-mentioned traditional Chinese medicine formula emulsion for treating multiple ear infections in animals in the preparation of drugs to improve abnormal blood routine in diseased animals; the blood routine includes white blood cells, red blood cells and hemoglobin.

[0014] In this invention, the traditional Chinese medicine formula emulsion for treating multiple ear infections in animals is composed of gallnut, coptis, rhubarb, sophora flavescens, cnidium monnieri, alum, phellodendron bark, Sichuan pepper, atractylodes macrocephala, borneol, and tea tree oil. Among them, gallnut has the effects of astringing and stopping bleeding, and absorbing moisture and healing sores; Coptis chinensis has the effects of clearing heat and drying dampness, purging fire and detoxifying; Rhubarb has the effects of cooling the blood and detoxifying, removing blood stasis and promoting menstruation; Sophora flavescens has the effects of clearing heat and drying dampness, killing parasites, and promoting diuresis; Phellodendron bark has the effects of clearing heat and drying dampness, purging fire and detoxifying; Cnidium monnieri has the effects of dispelling wind and detoxifying, and relieving itching; Alum has anti-inflammatory and drying properties; Sichuan pepper has the effects of warming the middle jiao and relieving pain, killing insects and relieving itching; Atractylodes macrocephala has the effects of tonifying qi, strengthening the spleen, drying dampness, promoting diuresis, and stopping bleeding; Borneol has the effects of clearing the orifices, dispersing stagnant heat, reducing swelling and relieving pain; Tea tree oil has cooling, detoxifying, and insecticidal effects.

[0015] The emergence of traditional Chinese medicine (TCM) compound formulations offers a new solution to this situation. Certain TCM compound formulations exhibit good killing effects against multiple pathogens causing various ear infections in animals, including bacteria, fungi, and mites. Furthermore, some components of these TCM compound formulations also promote the repair of ear canal mucosa and skin wounds, promote wound healing and tissue regeneration, reduce exudation, and accelerate scab formation. This helps repair ear canal tissue defects caused by prolonged scratching, inflammatory infiltration, or purulent damage, reducing the risk of secondary infection. Their multi-component, multi-target mechanism of action makes it difficult for pathogens to develop drug resistance. Moreover, the wide availability of natural products and low residue risk align with the current development direction of green farming and food safety, demonstrating their significant clinical application value and industrial prospects.

[0016] Beneficial Effects: Compared with the prior art, the present invention has the following significant advantages: 1. The traditional Chinese medicine formula emulsion of the present invention can effectively treat multiple ear infections in animals; the prepared emulsion has a convenient route of administration when used clinically; the traditional Chinese medicine formula emulsion can play multiple roles such as warming yang and dispelling cold, drying dampness and resolving stagnation, and strengthening the spleen and replenishing qi, helping infected animals to remove the accumulation of cold and dampness in their bodies, reducing damage to the external auditory canal of infected animals, improving the survival rate of infected animals and effectively restoring their growth performance; its therapeutic effect is superior to the treatment level of commonly used antibiotics in clinical practice; 2. The traditional Chinese medicine formula emulsion of the present invention provides a new option for the prevention and control of ear mites, bacteria and fungi in the process of livestock and poultry breeding, and is expected to reduce or replace the addition of antifungal drugs and insecticides in livestock and poultry breeding, alleviate the development of antibiotic resistance in livestock and poultry and reduce food safety issues such as drug residues. Attached Figure Description

[0017] Figure 1 These are comparative diagrams of multiple ear infections in rabbits in each experimental group before and after treatment in this embodiment of the invention; in the diagram, A is a comparative diagram of external auditory canal infections in rabbits in each experimental group before and after treatment; B is a comparative diagram of internal auditory canal infections in rabbits in each experimental group before and after treatment. Detailed Implementation

[0018] The specific technical solution of the present invention will be further described in detail below with reference to specific examples.

[0019] This invention discloses a traditional Chinese medicine formula emulsion for treating multiple ear infections in animals during large-scale farming. The formula comprises the following ingredients in proportion: 30 parts of Gallnut, 15 parts of Coptis chinensis, 15 parts of Rheum palmatum, 30 parts of Sophora flavescens, 30 parts of Cnidium monnieri, 10 parts of Alum, 10 parts of Phellodendron chinense, 10 parts of Zanthoxylum bungeanum, 10 parts of Atractylodes macrocephala, 5 parts of Borneol, and 10 parts of Tea Tree Oil. Except for the Tea Tree Oil, the ingredients to be decocted are added to 3 times their volume of 75% ethanol. Leaves and stems are soaked for 1.5 hours, while roots and fruits are soaked for 3 hours to ensure full ethanol absorption. The decoction is boiled at 55–60 °C, then reduced to 45–50 °C and decocted for 2 hours. The decoction is filtered through an 80-mesh sieve. 3 times their volume of 75% ethanol is added to the dregs, and the decoction is continued at 45–50 °C for 2 hours. The decoction is then filtered through an 80-mesh sieve. The two decoctions are combined, concentrated, and filtered to obtain 30... The dosage of the ethanol extract was 1 mg / mL. The dosage for each infected rabbit was adjusted daily according to its weight and the course of the disease.

[0020] This invention provides a formulation composed of traditional Chinese medicine. The emulsion of this invention has the effects of astringing and healing sores, clearing heat and drying dampness, purging fire and detoxifying, killing parasites and relieving itching, warming the middle and relieving pain. It has a good therapeutic effect on multiple ear infections in animals and has few toxic side effects.

[0021] Example 1: The therapeutic effect of a self-made traditional Chinese medicine emulsion on multiple infections of ear mites, bacteria, and fungi in animals. 1. Method 1.1 Laboratory Animals Several rabbits with ear infections were screened at a rabbit farm (Yigaole) in Yangzhou City. Thirty rabbits with mite infestation accompanied by mixed bacterial and fungal infections (confirmed by laboratory microscopic examination) and similar ear canal symptoms were selected. These rabbits were 5 months old and weighed 2-3 kg. They were then isolated and raised at the rabbit farm. 1.2 Preparation of self-made traditional Chinese medicine formula emulsion Traditional Chinese medicine composition A comprises 30 parts of Gallnut, 15 parts of Coptis chinensis, 15 parts of Rheum palmatum, 30 parts of Sophora flavescens, 10 parts of Phellodendron chinense, 30 parts of Cnidium monnieri, 10 parts of Alum, 10 parts of Zanthoxylum bungeanum, 10 parts of Atractylodes macrocephala, and 5 parts of Borneol. It has the effects of killing insects and relieving itching, clearing heat and detoxifying, and drying dampness and astringing sores. Traditional Chinese medicine composition B consists of 10 parts of Tea Tree Oil. It has the effects of cooling and detoxifying, killing insects and relieving itching. Traditional Chinese medicine composition A+B has the effects of astringing dampness and astringing sores, clearing heat and drying dampness, purging fire and detoxifying, killing insects and relieving itching, and warming the middle and relieving pain. Weigh out the proportioned Chinese herbal medicine compositions A, B, and A+B separately. Except for borneol, soak the other herbs in 3 times their volume of 75% ethanol. Soak the leaves and stems for 1.5 h, and the roots and fruits for 3 h. After boiling, decoct at low temperature for 2 h, and filter the decoction through an 80-mesh sieve. Add 3 times their volume of 75% ethanol to the residue and continue to decoct at low temperature for 2 h, then filter through an 80-mesh sieve. Combine the two decoctions and concentrate them to 1000 mL at low temperature. Add borneol at this point, decoct for 2 min, turn off the heat, filter, and freeze-dry the decoction. Prepare a 30 mg / mL alcohol extract from the freeze-dried powder using sterile water. Accurately weigh 0.8 g of tea tree oil, and slowly add 0.4 g of Tween 80 and 0.04 g of sodium carboxymethyl cellulose to the tea tree oil while stirring (1000 r / min) until completely dissolved. Then slowly add about 27 mL of alcohol extract and stir the mixture for another 10 min. Sonicate the mixture for 10 min at 400 W, 20 kHz and 40% power, frequency and amplitude to prepare a 30 mg / mL self-made traditional Chinese medicine emulsion. 1.3 Test drugs and strains All Chinese medicinal materials used in this invention were purchased from the market, and ivermectin solution (content 0.3%, batch number 201 80601) was purchased from a pharmaceutical company in a certain city.

[0022] The standard strain of Escherichia coli ATCC25922 was purchased from the China General Microbiological Culture Collection Center. Staphylococcus aureus standard strain ATCC29213 was purchased from the China General Microbiological Culture Collection Center. The clinical isolate of fungi causing rabbit ear disease was collected and isolated in our laboratory and later identified as Malassezia. 1.4 Methods 1.4.1 Preservation and culture conditions of Escherichia coli and Staphylococcus aureus Remove the frozen strain and thaw it. Add 20 µL to Luria-Bertani (LB) broth and incubate at 37°C for 180 rpm. -1 After culturing for 12-16 h, the bacteria were passaged. After 3-4 generations, the bacteria were inoculated onto LB agar plates using the three-zone streak method. The plates were then cultured in a bacterial incubator for 12-16 h. After single colonies were formed, a single colony was picked and added to LB broth medium. The medium was placed in a 37 ℃ constant temperature shaker and multiplied to the logarithmic growth phase for use in experiments. 1.4.2 Identification of fungi causing rabbit ear infections Using a 1 μL inoculation loop, pick up a suitable freshly cultured clinically isolated fungal colony, spread the fungal colony evenly in the center of the target site to form a thin layer, add 2 μL of solution B with a pipette, and after it dries naturally, add 1.5 μL of solution A in the center of the target site, let it dry naturally, and then use the QuanID microbial mass spectrometry system to detect the fungal species. 1.4.3 Preservation and Culture Conditions of Malassezia Take out the frozen strain and thaw it. Dip the inoculation loop into the bacterial solution and inoculate the fungus into potato agar (PDA) using the three-zone streak method. Incubate at 37 ℃ for two weeks to form visible pale yellow single colonies, which can then be used for experiments. 1.4.4 Selection of Rabbit Itch Mite Cases (1) Diagnostic criteria: The external ear canal of the diseased rabbit has inflammatory exudate, which dries and forms a yellow scab that is embedded in the ear canal or even blocks the ear canal like a paper roll. The ears of the diseased rabbit droop, shakes its head from time to time, scratches its ears, and observes the presence of rabbit itch mites under a microscope after scraping off the scab. (2) Inclusion criteria: Meeting relevant clinical diagnostic criteria; (3) Exclusion criteria: having other skin diseases and having already received drug treatment; 1.4.5. Case grouping and treatment Fifty rabbits with prurigo mite disease meeting the inclusion criteria were randomly divided into five groups (n=10 per group) according to their order of consultation: Model group, Traditional Chinese Medicine Composition A group (TCM-A), Traditional Chinese Medicine Composition B group (TCM-B), Traditional Chinese Medicine Composition A+B group (TCM-A+B), and Positive Drug Ivermectin (IVM) group. The Traditional Chinese Medicine Composition group received topical application once daily for 3 days, while the Positive Drug group received topical application of ivermectin solution once daily for 10 days. The experimental period was 10 days. 1.5 Determination of the minimum inhibitory concentration and minimum bactericidal concentration of the self-prepared traditional Chinese medicine emulsion against Escherichia coli and Staphylococcus aureus According to the Clinical Laboratory Standards Institute (CLSI) standard (M100, 2019), this experiment used the broth microdilution method to determine the minimum inhibitory concentration (MIC) of a self-prepared traditional Chinese medicine formula against *Escherichia coli* and *Staphylococcus aureus*. The self-prepared traditional Chinese medicine formula emulsion was added to sterile 96-well bacterial culture plates, and then diluted to a specific concentration using MH broth using a two-fold dilution method. The concentrations of the traditional Chinese medicine solution in wells 1-9 were 30, 15, 7.5, 3.75, 1.88, 0.94, 0.47, 0.23, and 0.12 mg / mL, respectively. Then, 100 µL of bacterial suspension was added to each well from wells 1-10. Well 10 served as a positive control, and well 11 as a negative control. *Escherichia coli* and *Staphylococcus aureus* in the logarithmic growth phase were diluted with MH broth to a concentration of 2.0 × 10⁻⁶. 6 CFU / mL, 100 μL of bacterial suspension of the test strain was inoculated into the wells after drug addition; drug negative control wells, MH broth negative control wells and bacterial positive control wells were set up, with 3 replicates for each well; the 96-well bacterial culture plate was placed in a bacterial incubator at 37℃ for 16-18 h, and the result was determined by the concentration at which no bacterial growth was found in the well, which was recorded as the MIC of the traditional Chinese medicine formula emulsion for this strain; the minimum bactericidal concentration (MBC) was determined using the plate diffusion test method, 10 μL of MH broth culture corresponding to each concentration in the bacterial culture plate was dropped onto an LB agar plate, and after incubation at 37 ℃ for 24 h, the concentrations corresponding to the formation of colonies on the plate were counted, and the lowest concentration corresponding to no colony formation was recorded as the MBC. 1.6 Determination of the minimum inhibitory concentration of self-prepared traditional Chinese medicine emulsion against Malassezia Malassezia was inoculated onto potato dextrose agar (PDA) and incubated at 28 °C for 14 days to activate it. Activated colonies were picked from the culture plate and 2–3 mL of RPMI 1640 liquid medium was added and agitated. The colonies were then transferred to 2 mL sterile centrifuge tubes and shaken for 15 seconds. After counting with a hemocytometer, the bacterial concentration was adjusted to (1–3) × 10⁻⁶ using RPMI 1640 liquid medium. 5CFU / mL was prepared for use; RPMI 1640 liquid medium was added to a 96-well plate, 100 µL to wells 1-10 and 200 µL to well 12. The prepared herbal decoction was added to well 1, mixed with the medium by pipetting, and then 100 µL was added to well 2. This was serially diluted to well 9, so that the concentrations of the herbal decoction in wells 1-9 were 30, 15, 7.5, 3.75, 1.88, 0.94, 0.47, 0.23, and 0.12 mg / mL, respectively. Then, 100 µL of bacterial suspension was added to each well 1-10. Well 10 served as a positive control and well 11 served as a negative control. The 96-well plate was incubated at 28 °C for 7 days. The lowest drug concentration at which no fungal growth was observed visually, assuming the control wells showed correct results, was the endpoint of the minimum inhibitory concentration (MIC). 1.7 Detection Indicators 1.7.1 Infection Severity and Scoring During the experiment, the rabbits' appetite, feces, urine, and mental state were observed daily for any abnormalities. On days 0, 4, and 10 of the experiment, the ear inflammation exudate and crusting were examined, and mites were detected under a microscope. The degree of infection was scored as follows: no exudate or mites: 0 points; ear canal exudate but no mites: 0.5 points; ear canal with a small amount of crust and mites: 1 point; external ear canal filled with crust and mites: 2 points; ear canal and proximal 1 / 4 of the auricle with crust and mites: 3 points; 1 / 2 of the auricle filled with crust and mites: 4 points; 3 / 4 of the auricle filled with crust and mites: 5 points; the entire auricle filled with crust and mites: 6 points. The average scores of different experimental groups were calculated and statistically analyzed. 1.7.2. Parasite reduction rate and parasite negative conversion rate On days 0, 4 and 10 of the experiment, scabs from the affected area were collected, placed in a clean petri dish, crushed and mixed with tweezers, and about 0.1 g was placed in a 35℃ incubator for 0.5 to 1 h. The mites were collected and counted, and the mites reduction rate for each experimental rabbit and the mites conversion rate for different experimental groups were calculated. ; 1.8 Criteria for Evaluating Therapeutic Effect On day 10 of the experiment, the number of ear mites in each rabbit was checked, the reduction rate of mites was calculated, and the efficacy was judged in combination with clinical symptoms. Effective: The average reduction rate of ear mites was greater than or equal to 90%, and there was no itching, no exudate, no scabs or only a small amount of scabs in the affected area. Ineffective: The average reduction rate of ear mites was less than 90%, and there was itching, exudate, and scabs in the affected area. ; 1.9 Data Processing and Analysis Statistical analysis was performed using IBM SPSS Statistics 27.0 software, and graphs were created using Graphpad Prism 8.0.3. All data are expressed as mean ± standard deviation (Mean ± SEM). One-way ANOVA was used for comparisons between groups, and significant differences were found between different letters.

[0023] Experimental results 2.1 Determination of the minimum inhibitory concentration and minimum bactericidal concentration of the self-made traditional Chinese medicine emulsion against Escherichia coli and Staphylococcus aureus; see Table 1 for details; Table 1. MIC and MBC of TCM group against Escherichia coli and Staphylococcus aureus

[0024] As shown in Table 1, the MIC of TCM-A against ATCC25922 was 1.88 mg / mL, and the MBC was 3.75 mg / mL; the MIC of TCM-B against ATCC29213 was 3.75 mg / mL, and the MBC was 7.5 mg / mL; the MIC of TCM-B against ATCC25922 was 3.75 mg / mL, and the MBC was 7.5 mg / mL; the MIC of TCM-A+B against ATCC25922 was 0.94 mg / mL, and the MBC was 1.88 mg / mL; the MIC of TCM-A+B against ATCC25922 was 1.88 mg / mL, and the MBC was 3.75 mg / mL. 2.2 Determination of the minimum inhibitory concentration of the traditional Chinese medicine formulation against Malassezia; see Table 2 for details; Table 2 MIC of TCM group against Malassezia

[0025] As shown in Table 2, the MIC of TCM-A group against clinical strains of Malassezia was 15 mg / mL, the MIC of TCM-B group against clinical strains of Malassezia was 30 mg / mL, and the MIC of TCM-A+B group against clinical strains of Malassezia was 7.5 mg / mL. 2.3 Clinical symptom scores for each treatment period; see Table 3 for details; Table 3 Clinical symptom scores for each experimental group

[0026] As shown in Table 3, after treatment, the severity and score of multiple ear infections decreased in the TCM-A group from 3.58 ± 0.35 to 2.56 ± 0.72, and in the TCM-B group from 3.71 ± 0.26 to 2.84 ± 0.48. There was no significant difference compared to the Model group. P >0.05); the severity and score of multiple ear infections decreased in the TCM-A+B group from 4.00 ± 0.00 to 0.17 ± 0.24, and in the IVM group from 3.33 ± 0.47 to 1.33 ± 0.47. There was no significant difference in symptom scores between the two groups. P >0.05); 2.4 Effects of the self-prepared traditional Chinese medicine emulsion on the reduction rate and negative conversion rate of ear worms in animals of each experimental group; see Table 4 for details; Table 4. Number of negative cases, negative conversion rate, and reduction rate of parasites in each experimental group

[0027] As shown in Table 4, the TCM-A group treated 10 rabbits with pruritus mite infection achieved a 40% negative conversion rate, the TCM-B group achieved a 20% negative conversion rate, and the TCM-A+B group achieved a 100% negative conversion rate; the treatment effect was comparable to that of the IVM group. P >0.05); 2.5 Effects of Traditional Chinese Medicine Formulas on Hematological Indicators; see Table 5 for details; Table 5 Hematological indicators of each experimental group

[0028] As shown in Table 5, on day 10 of the experiment, the hematological parameters such as WBC, Lym, Gra, RBC, and HGB in the Model group were significantly different from those in the Control group. P <0.01), there was no significant difference between the TCM group and the IVM group ( P >0.05), with no significant difference compared to the Control group ( P >0.05), indicating that the self-made traditional Chinese medicine preparation has no significant abnormal effect on hematological indicators and has a good therapeutic effect; 2.6 The therapeutic effect of self-prepared traditional Chinese medicine emulsion on rabbit ear disease After treatment with the traditional Chinese medicine combination, the rabbits' ear symptoms showed significant improvement, itching disappeared, and scabs fell off (e.g. Figure 1 Microscopic examination revealed a small number of mites in a very few experimental rabbits, while the number of mites in the remaining experimental rabbits decreased to 0.

[0029] 3. Experimental Results The traditional Chinese medicine (TCM) emulsion has demonstrated high efficacy in treating multiple ear infections in animals, significantly alleviating infection-related clinical symptoms and promoting healthy skin recovery. Under local administration, the TCM emulsion exhibits good safety with no significant adverse reactions in animals with multiple ear infections. Furthermore, the TCM emulsion effectively inhibits the growth of common pathogens, including Escherichia coli, Staphylococcus aureus, and Malassezia, thus exerting antibacterial effects while treating multiple ear infections. This indicates that the TCM emulsion accelerates the recovery process of ear infections through multiple mechanisms, including direct antiparasitic action, improvement of skin microecology, and enhanced local antibacterial capacity. Compared with existing chemical drugs (such as ivermectin), the TCM emulsion achieves comparable therapeutic efficacy while offering better safety and ease of application, providing a highly effective, economical, and safe natural drug alternative for the local treatment of ear infections in animals.

Claims

1. A traditional Chinese medicine emulsion for treating multiple ear infections in animals, characterized in that, The traditional Chinese medicine composition consists of traditional Chinese medicine composition A and traditional Chinese medicine composition B; The composition of the traditional Chinese medicine A is as follows: 28-32 parts of Gallnut, 13-17 parts of Coptis chinensis, 13-17 parts of Rheum palmatum, 28-32 parts of Sophora flavescens, 8-12 parts of Phellodendron chinense, 28-32 parts of Cnidium monnieri, 8-12 parts of Alum, 8-12 parts of Zanthoxylum bungeanum, 8-12 parts of Atractylodes macrocephala, and 3-7 parts of Borneol. The mass composition of the traditional Chinese medicine composition B is as follows: 8-12 parts of tea tree oil.

2. The traditional Chinese medicine emulsion for treating multiple ear infections in animals according to claim 1, characterized in that, The composition of the traditional Chinese medicine is as follows: 30 parts of Gallnut, 15 parts of Coptis chinensis, 15 parts of Rheum palmatum, 30 parts of Sophora flavescens, 10 parts of Phellodendron chinense, 30 parts of Cnidium monnieri, 10 parts of Alum, 10 parts of Zanthoxylum bungeanum, 10 parts of Atractylodes macrocephala, 5 parts of Borneol and 10 parts of Tea Tree Oil.

3. The method for preparing the traditional Chinese medicine formula emulsion for treating multiple ear infections in animals as described in any one of claims 1-2, characterized in that, Includes the following steps: Step (1) Soak the raw materials to be decocted, excluding tea tree oil, in 3 times their volume of 75% ethanol. After soaking in step (2), the raw material is heated to boiling in a rotary evaporator at a water bath temperature of 55–60 ℃. After boiling, the water bath temperature is reduced to 45–50 ℃ and the decoction is continued. After decoction, the dregs are filtered through an 80-mesh filter and the decoction is collected. The filtered dregs are used for decoction again. Step (3) Add 3 times the volume of 75% ethanol to the filtered residue. Use a rotary evaporator to heat the soaked raw material to boiling in a water bath at a temperature of 55–60 ℃. After boiling, reduce the water bath temperature to 45–50 ℃ and continue decocting. After decocting, filter the residue through an 80-mesh filter and discard it. Collect the decoction. Combine the two decoctions and use a low flame to concentrate the decoction to the same volume as the raw material to make the final alcohol extract.

4. The method for preparing the traditional Chinese medicine formula emulsion for treating multiple ear infections in animals according to claim 3, characterized in that, The soaking time mentioned in step (1) is as follows: 1.5 h for leafy and stem drugs, and 3 h for drugs mainly composed of roots and fruits.

5. The method for preparing the traditional Chinese medicine formula emulsion for treating multiple ear infections in animals according to claim 3, characterized in that, The time for continued low-temperature simmering in step (2) is 2 hours; The time for continued low-temperature simmering in step (3) is 2 hours.

6. The application of a traditional Chinese medicine formula emulsion for treating multiple ear infections in animals prepared according to the preparation method described in claim 3 in reducing the bacterial content in multiple ear infections in animals.

7. The application of a traditional Chinese medicine formula emulsion for treating multiple ear infections in animals prepared according to the preparation method described in claim 3 in reducing the fungal content in multiple ear infections in animals.

8. The application of a traditional Chinese medicine formula emulsion for treating multiple ear infections in animals prepared according to the preparation method described in claim 3 in reducing the number of itch mites in multiple ear infections in animals.

9. The application according to claim 8, characterized in that, The reduction of the number of itch mites refers to: observing the number of itch mites in the ears of experimental animals daily; in, ; 。 10. The application of a traditional Chinese medicine formula emulsion for treating multiple ear infections in animals prepared by the preparation method according to claim 3 in the preparation of drugs to improve abnormal blood routine indicators of multiple ear infections in animals.

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