A soothing sleep red ganoderma fig leaf lotus liquor, preparation method and application

Abstract

The application discloses a red ganoderma lingzhi and fargesia murielii liqueur for soothing nerves and helping sleep, a preparation method and application thereof, and belongs to the technical field of biology, medicine and food. The raw materials for preparing the red ganoderma lingzhi and fargesia murielii liqueur include red ganoderma lingzhi, fargesia murielii, medlar and wine base, the red ganoderma lingzhi, the fargesia murielii and the medlar are concentrated after being extracted by water, and then are mixed with the base wine, and finally are treated by clarification to obtain the red ganoderma lingzhi and fargesia murielii liqueur. Experiments show that the red ganoderma lingzhi and fargesia murielii liqueur can significantly prolong the sleep time of insomnia mice induced by sodium pentobarbital, shorten the sleep latency, and improve the sleep rate; can improve the open field behavior abnormality of the insomnia mice; and can improve the GABA and 5-HT contents in the brain. The red ganoderma lingzhi and fargesia murielii liqueur has the advantages of simple formula, controllable process, good safety and the like.

Description

Technical Field

[0001] This invention belongs to the fields of biology, medicine, and food technology, and specifically relates to a soothing and sleep-aiding Ganoderma lucidum and five-finger peach liqueur, its preparation method, and its application. Background Technology

[0002] Liqueurs have a long history in my country. They are distinctive beverages made by blending, mixing, and processing distilled spirits, fermented wines, or edible alcohol as the base, and adding edible or medicinal substances or food additives as auxiliary materials.

[0003] Currently, the mainstream preparation method for liqueurs in the industry is to pulverize the raw materials and soak them at room temperature for a long time. Related existing technologies, such as the American ginseng and astragalus liqueur disclosed in patent CN120505159A, use a long-term room temperature static leaching process to soak Chinese medicinal materials for 20 to 40 days to obtain the extract.

[0004] The aforementioned traditional maceration process has several technical drawbacks: First, static maceration at room temperature has low efficiency and low raw material utilization; second, prolonged maceration easily causes a large amount of tannins and bitter tannins to precipitate out, resulting in a bitter taste in the finished product, poor palatability, and a limited target audience; third, the maceration cycle can last for dozens of days, resulting in slow production turnover and hindering industrial mass production; fourth, after blending, the liqueur is prone to flocculation and sedimentation during storage, leading to poor light transmittance and a short shelf life.

[0005] Insomnia is a common sleep disorder characterized by difficulty falling asleep, sleep maintenance difficulties, early awakening, and impaired daytime functioning. According to the International Classification of Diseases, 11th Revision (ICD-11) and the Chinese Guidelines for the Diagnosis of Insomnia, chronic insomnia has become the second most prevalent mental health problem worldwide. Insomnia is characterized by difficulty falling asleep, sleep maintenance difficulties, early awakening, and decreased sleep quality. Long-term insomnia can lead to serious consequences such as fatigue, decreased attention, mood instability, and impaired immune function. The occurrence of insomnia involves multiple interconnected factors, including imbalances in neurotransmitters in the central nervous system (5-HT, GABA, Glu, etc.), an imbalance in the excitation / inhibition ratio, cellular oxidative stress and inflammatory responses, and activation of the hypothalamus-pituitary-adrenal (HPA) axis.

[0006] The current liqueur market has enormous potential, but product variety is limited, and the lack of innovative taste makes it difficult to attract young consumers. Most liqueurs rely on a single medicinal herb for infusion, resulting in limited efficacy and a lack of multi-target synergistic regulation. Furthermore, high production costs and lengthy production cycles severely restrict industry development. There is an urgent need to develop liqueurs with calming, sleep-aiding, and neuroprotective functions, a good taste, and simple preparation methods. Summary of the Invention

[0007] The purpose of this invention is to provide a soothing and sleep-aiding Ganoderma lucidum and five-finger peach liqueur, its preparation method, and its application, in order to solve the problems existing in the prior art.

[0008] To achieve the above objectives, the present invention provides the following solution: This invention provides a Ganoderma lucidum and Prunus cerasifera liqueur with calming, sleep-aiding, and neuroprotective functions. The ingredients include Ganoderma lucidum, Prunus cerasifera var. sarcodactylis, wolfberry, and base liquor.

[0009] Red Ganoderma is the dried fruiting body of the fungus *Ganoderma lucidum* or *Ganoderma sinense*, belonging to the Polyporaceae family. It is sweet and slightly warm in nature, and enters the liver and kidney meridians. Its main active components are Ganoderma lucidum polysaccharides and triterpenes. Ganoderma lucidum regulates the release of neurotransmitters in the central nervous system, inhibits the release of norepinephrine through the 5-HT degradation pathway, triggering a cascade regulatory effect of the "neuro-immune-endocrine" pathway, thereby improving sleep disorders such as anxiety and insomnia. In a specific embodiment of this invention, the red Ganoderma lucidum is preferably *Ganoderma lucidum* from Hainan. The *Ganoderma lucidum* from Hainan in this invention refers to red Ganoderma lucidum grown or cultivated in Hainan Province.

[0010] Five-finger fig (Ficus hirta) is the dried root of the Ficus hirta plant, belonging to the Moraceae family. It is sweet and slightly warm in nature, and enters the lung and spleen meridians. It invigorates qi and strengthens the spleen, resolves phlegm and relieves cough, and promotes qi circulation and eliminates dampness. The flavonoids and coumarins in five-finger fig can regulate the balance of intestinal flora, improve anxiety, and indirectly improve sleep quality by regulating spleen and stomach function.

[0011] Goji berries are the dried, ripe fruit of *Lycium barbarum*, a plant in the Solanaceae family. They are sweet and neutral in nature, and enter the liver and kidney meridians. They nourish the liver and kidneys, benefit essence and improve eyesight. Goji berry polysaccharides have antioxidant, immunomodulatory, neuroprotective, liver-protective, eye-protective, and glucose and lipid metabolism-regulating effects.

[0012] The mouse experiments conducted using the *Ganoderma lucidum* and *Ficus hirta* liqueur prepared according to this invention showed that it significantly prolonged sleep time, shortened sleep latency, and increased sleep onset rate in mice with pentobarbital-induced insomnia; it also improved open field behavior abnormalities in insomnia mice; and it increased the levels of GABA and 5-HT in the brain. The *Ganoderma lucidum* and *Ficus hirta* liqueur of this invention has advantages such as simple formulation, controllable process, and good safety.

[0013] Optionally, the mass ratio of the Ganoderma lucidum, Prunus cerasifera var. rubra and Lycium barbarum is (10-12):(22.5-24):(7-9).

[0014] Optionally, the alcohol content of the Ganoderma lucidum and Prunus cerasifera liqueur is 35% vol-40% vol; the medicinal materials in the Ganoderma lucidum and Prunus cerasifera liqueur include Ganoderma lucidum, Prunus cerasifera var. chinensis and Lycium barbarum.

[0015] This invention provides a method for preparing a liqueur containing Ganoderma lucidum and Prunus cerasifera, wherein the liqueur uses a light-aroma baijiu as its base, and the base contains aqueous extracts of traditional Chinese medicines prepared from Ganoderma lucidum, Prunus cerasifera, and Lycium barbarum. The light-aroma baijiu of this invention conforms to the GB / T 10781.2-2022 standard.

[0016] Optionally, the preparation method of the aqueous extract of the traditional Chinese medicine is as follows: S1. Use Ganoderma lucidum, five-finger peach and wolfberry as raw materials, wash and dry them, and soak them in water for 30-35 minutes. S2. Add water to the soaked raw materials and boil them for the first and second times. Let the combined filtrate stand and settle naturally to obtain the supernatant. S3. The supernatant is concentrated to obtain a water extract of traditional Chinese medicine. Optionally, the supernatant is concentrated to 1 / 10 of its original volume, i.e., the concentration factor is 10 times.

[0017] Optionally, the light-aroma baijiu is mixed with water and adjusted to the target alcohol content to obtain a blended base liquor; the blended base liquor is then mixed with the herbal water extract to obtain a preliminary liqueur. The amount of water added in the first decoction is 9-10 times the total mass of the raw materials Ganoderma lucidum, Ficus hirta, and Lycium barbarum; the amount of water added in the second decoction is also 9-10 times the total mass of the raw materials Ganoderma lucidum, Ficus hirta, and Lycium barbarum.

[0018] Optionally, the volume ratio of the herbal extract to the light-aroma baijiu in the initial liqueur is 5:27.

[0019] Optionally, flavoring agent and clarifying agent are added to the initial liqueur to obtain the Ganoderma lucidum and Prunus cerasifera syrup; the volume ratio of the herbal extract to the flavoring agent is (20-100):(3-9).

[0020] Optionally, the flavoring agent includes glycerin, and the clarifying agent includes a chitosan solution with a concentration of 10-20 g / L, wherein the amount of chitosan solution added is 10% of the volume of the liqueur.

[0021] This invention provides the application of Ganoderma lucidum and Prunus cerasifera syrup in at least one of the following: 1) Prepare calming and sleep-aiding products; 2) Prepare neuroprotective products.

[0022] The present invention discloses the following technical effects: 1. The Ganoderma lucidum and Prunus cerasifera syrup of this invention has a clear body, delicate taste, and mellow flavor.

[0023] 2. This invention provides a method for preparing a Ganoderma lucidum and Prunus cerasifera liqueur. Using Ganoderma lucidum, Prunus cerasifera var. chinensis, and Lycium barbarum as traditional Chinese medicine raw materials, the resulting aqueous extract of these herbs after decoction has a high content of effective components, high material utilization rate, high solubility, and high relative stability. Then, using a light-aroma baijiu as a base, a concentrated extract of Ganoderma lucidum liqueur is added to prepare the liqueur (prepared liquor). This liquor has stable quality, a full-bodied and delicate texture, and a pure taste. Long-term consumption has effects such as calming the nerves, aiding sleep, reducing anxiety, and protecting the nervous system.

[0024] 3. The present invention uses the water extract of Ganoderma lucidum and Prunus cerasifera and blended base liquor (the light-aroma baijiu and water are mixed to obtain the blended base liquor) to form the liqueur of the present invention. The process from raw materials to finished product can be completed in as little as three days. It has the advantages of simple operation, wide adaptability and low cost.

[0025] 4. This invention is the first to improve the herbal water extracts of Ganoderma lucidum, Prunus cerasifera, and Lycium barbarum into a liquor preparation. The liquor base is light-aroma baijiu, and the herbal water extracts of Ganoderma lucidum, Prunus cerasifera, and Lycium barbarum are dispersed in the liquor base to form a compound liquor product with functions such as calming the nerves and promoting sleep, anti-anxiety, and repairing damage to the central nervous system.

[0026] 5. The aqueous extracts of the traditional Chinese medicines of this invention (Ganoderma lucidum-Ficus hirta-Lycium barbarum aqueous extracts) have been shown to have neuroprotective effects in in vitro cell experiments. An acute injury model of hippocampal neurons in HT22 mice was constructed using 35 mmol / L glutamate. After treatment with different concentrations of the aqueous extracts, cell survival increased in a concentration-dependent manner, indicating that the aqueous extracts of the traditional Chinese medicines of this invention have a significant repair and protective effect on glutamate-induced neuronal damage. This provides cellular evidence of its calming, sleep-aiding, and neuroprotective effects, and offers in vitro experimental evidence for the efficacy evaluation of the liquor product.

[0027] 6. The Ganoderma lucidum, Prunus radix et Rhizoma, and Lycium barbarum liqueur of the present invention significantly increased the content of γ-aminobutyric acid (GABA) and 5-hydroxytryptamine (5-HT) in mouse brain tissue (P<0.05), suggesting that the liqueur can participate in the systemic regulation of central neurotransmitter balance through the gut-brain axis. It can be inferred that the Ganoderma lucidum, Prunus radix et Rhizoma, and Lycium barbarum liqueur upregulates the expression of GABA_A receptor α1 subunit and enhances the influx of postsynaptic chloride ions through Ganoderma triterpenes, while promoting the recovery of the expression of 5-HT synthesis rate-limiting enzyme TPH2, thereby achieving dual positive regulation of the GABAergic inhibitory pathway and the 5-HTergic regulatory pathway, and correcting the PCPA-induced neurotransmitter depletion state at the molecular level. Attached Figure Description

[0028] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0029] Figure 1 This is a schematic diagram illustrating the repair effect of the aqueous extract of traditional Chinese medicine (Ganoderma lucidum-Ficus hirta-Lycium barbarum aqueous extract) on glutamate-damaged HT22 cells in this invention; Figure 2 This is a schematic diagram of the mouse weight measurement results in this invention; Figure 3This is a schematic diagram of the sodium pentobarbital sleep results in mice in this invention; Figure 4 This is a schematic diagram of the mouse open field experiment movement trajectory measurement results in this invention; Figure 5 This is a schematic diagram showing the results of 5-HT content determination in mouse brain tissue in this invention; Figure 6 This is a schematic diagram showing the results of GABA content measurement in mouse brain tissue in this invention; In the above figure, ### represents a highly significant difference compared to the control group. p <0.001); * indicates a significant difference compared to the model group ( p <0.05); ** indicates a highly significant difference compared to the model group ( p <0.01); *** indicates a highly significant difference compared to the model group ( p <0.001). Detailed Implementation

[0030] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0031] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0032] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0033] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0034] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0035] Hainan Ganoderma refers to Ganoderma lucidum that grows or is cultivated in Hainan Province. The Ganoderma lucidum used in the examples is Hainan Ganoderma lucidum.

[0036] Example 1: Preparation of Basic Formula (1) Preparation of Ganoderma lucidum-Ficus hirta-Lycium barbarum aqueous extract (Chinese herbal water extract): 11.6 g of Ganoderma lucidum, 23.5 g of Ficus hirta, and 8.4 g of Lycium barbarum were used as raw materials. They were washed, dried, and soaked in 435 mL of distilled water for 30 min. The soaked raw materials were decocted in a clay pot, boiled over high heat for 15 min, and then boiled over low heat for 30 min. After that, the mixture was filtered through gauze. The residue was then boiled again with 435 mL of distilled water. The temperature and time of the second decoction were as described above. After the second decoction, the mixture was coarsely filtered through gauze. The filtrates from the two decoctions were combined and allowed to settle naturally for 2 h. The supernatant obtained from the natural settling of the filtrate was added to a rotary evaporator and concentrated 10 times by rotary evaporation to obtain the Chinese herbal water extract.

[0037] (2) To prepare 99.9 mL of 35% vol liqueur: take 54 mL of 65° light aroma baijiu, add 35 mL of purified water and 10 mL of Chinese herbal extract, and mix well. Based on comprehensive sensory evaluation, glycerol is selected as a flavoring agent and added to the above liqueur system. After mixing for 3 min, it is bottled to obtain Ganoderma lucidum and Prunus cerasifera var. rubra ...

[0038] (3) Because the Chinese herbal extracts added to the liqueur prepared above contain precipitated components, the liqueur becomes cloudy to a certain extent and is not clear. In order to improve the clarity of the liqueur, chitosan is selected as a clarifying agent to clarify the liqueur. Finally, the clarifying agent is determined to be a chitosan solution with a concentration of 10 g / L.

[0039] Preparation method of chitosan solution: Weigh 1g of chitosan into a beaker, stir thoroughly with 89 mL of 55℃ pure water, transfer to a volumetric flask and make up to 100 mL, let stand for 24 h.

[0040] Accurately measure the prepared Ganoderma lucidum and five-finger peach liqueur, add 10 g / L chitosan solution, and let it settle naturally at room temperature (24℃) for 24 h; the volume ratio of Ganoderma lucidum and five-finger peach liqueur to chitosan solution is 10:1. Then take the supernatant, filter it, and obtain the clarified Ganoderma lucidum and five-finger peach liqueur.

[0041] Example 2: Preparation of different amounts of concentrated solution Compared with Example 1, the difference is that the amount of purified water added in step (2) is increased. For each 99.9 mL of 35% vol liqueur, 54 mL of 65° light aroma baijiu, 39 mL of purified water, 6 mL of Chinese herbal extract, and 0.9 mL of glycerol are required. The volume ratio of Chinese herbal extract to glycerol is 20:3.

[0042] Example 3: Preparation of different amounts of concentrated solution Compared with Example 1, the difference is that the amount of purified water added in step (2) is increased. For every 99.9 mL of 35% vol liqueur, 54 mL of 65° light aroma baijiu is required, the amount of purified water added is 37 mL, the amount of Chinese herbal extract added is 8 mL, the amount of glycerol added is 0.9 mL, and the volume ratio of Chinese herbal extract to glycerol is 80:9.

[0043] Example 4: Preparation of different amounts of concentrated solution Compared with Example 1, the difference lies in step (2) adjusting the amount of purified water added. For each 99.9 mL 35% vol liqueur prepared, 54 mL of 65° light aroma baijiu, 33 mL of purified water, 12 mL of Chinese herbal extract, and 0.9 mL of glycerol are required. The volume ratio of Chinese herbal extract to glycerol is 40:3.

[0044] Experiment 1: The Repairing Effect of Aqueous Extracts of Traditional Chinese Medicine on Cell Damage An acute injury model of HT22 cells was constructed using 35 mmol / L (35M) glutamate (Glu). HT22 cells in logarithmic growth phase (8000 cells / well) were seeded into 96-well plates and cultured at 37°C in a 5% CO2 incubator until adherence. The cells were then divided into groups of five replicates, each treated with different concentrations of aqueous extracts of traditional Chinese medicine. The aqueous extract of traditional Chinese medicine prepared in Example 1 was first freeze-dried into powder, and then prepared into aqueous extract solutions of 0.4 mg / mL, 0.8 mg / mL, and 1.6 mg / mL.

[0045] Control group (CON): Normal HT22 adherent cells were treated with medium containing 10% FBS for 24 h; Model group (GLU): Normal HT22 adherent cells treated with 35M glutamate for 24h; Treatment group 1 (0.4): Normal HT22 adherent cells, treated with a traditional Chinese medicine aqueous extract solution and 35M for 24h, with the concentration of the traditional Chinese medicine aqueous extract solution being 0.4mg / mL; Treatment group 2 (0.8): Normal HT22 adherent cells, treated with a traditional Chinese medicine aqueous extract solution and 35M glutamate for 24 h; the concentration of the traditional Chinese medicine aqueous extract solution was 0.8 mg / mL; Treatment group 3 (1.6): Normal HT22 adherent cells, treated with a traditional Chinese medicine aqueous extract solution and 35M glutamate for 24 h; the concentration of the traditional Chinese medicine aqueous extract solution was 1.6 mg / mL; After treatment, cell viability was detected using the CCK-8 assay to evaluate the repair effect of the aqueous extract of traditional Chinese medicine on glutamate-induced cell damage. Results are as follows: Figure 1 As shown.

[0046] Experimental results showed that, compared with the glutamate-induced injury (Glu) model group, the cell survival rate increased in a concentration-dependent manner in the treatment groups given different concentrations of the herbal aqueous extract solution. At concentrations of 0.4 mg / mL, 0.8 mg / mL, and 1.6 mg / mL, cell survival rate was significantly improved, indicating that the herbal aqueous extract has a significant protective effect against glutamate-induced HT22 cell damage. This suggests that the herbal aqueous extract prepared in this invention has neuroprotective activity in vitro, providing cellular-level experimental evidence for its calming and sleep-aiding effects.

[0047] Experiment 2: Sensory Evaluation In accordance with the relevant requirements for sensory evaluation in the "Liqueur" standard T / CBJ 9101-2021, a sensory evaluation team consisting of 10 uniformly trained evaluators was formed to conduct sensory evaluation on the five indicators of color, clarity, aroma, taste and style of the liqueur made from Ganoderma lucidum and Prunus cerasifera (Liqueur) in Examples 1, 2, 3 and 4 of this invention. Each indicator was assigned three levels: excellent, good and poor. The results are shown in Table 1.

[0048] The fuzzy mathematical sensory evaluation system is constructed as follows: Factor set (U): Composed of sensory evaluation indicators, i.e., U = {color (U1), clarity (U2), aroma (U3), taste (U4), style (U5)}.

[0049] The evaluation set (V) is the set of grades given by the evaluators for each factor, set as V = {Excellent (V1), Good (V2), Poor (V3)}.

[0050] Weight set (W): The weights of each indicator are determined by user survey and binary comparison method. The weight vector is assigned the value W=(0.2,0.1,0.2,0.3,0.2), which correspond to color, clarity, aroma, taste and style respectively.

[0051] Divide the frequency of each comment set by 10, and then construct a fuzzy relation matrix.

[0052] Mk= Where k = 1, 2, ..., m are the sample numbers.

[0053] Comprehensive evaluation model: based on the fuzzy mathematics evaluation model T=W×M k Calculate the comprehensive evaluation vector for sample k. Assign values ​​to the comment set using a weighted average method (Excellent = 3 points, Good = 2 points, Poor = 1 point) and calculate the mean of the comprehensive scores. Finally, rank all samples according to their scores.

[0054] Table 1 Evaluation results of the liqueur ratio in this invention (unit: points) As can be seen from the sensory evaluation table, the Ganoderma lucidum and Prunus cerasifera liqueur of Example 1 has a refreshing taste, rich medicinal aroma, full body, and bright color, and has a typical style.

[0055] Experiment 3: Stability Test The finished product prepared in Example 1 was subjected to four extreme conditions for 10 days: exposure to sunlight at 30°C, exposure to high temperature at 60°C, shaking (37°C, 220 rpm), and freezing at -18°C. The stability of the samples was studied, and changes in product stability were observed. The results are shown in Table 2. The transmittance (T) of the wine was determined spectrophotometrically: using distilled water as a blank correction baseline, the OD value (A) of the wine sample was measured at a wavelength of 600 nm, according to the formula [T(%) = 10]. -A [×100] Transmittance is used to characterize the clarity of the wine; the higher the percentage of transmittance, the better the clarification. Among them, the clarity of the Ganoderma lucidum liqueur after 10 days at high temperature is only 63.78%, so the liqueur has the worst stability under long-term high temperature conditions, as shown in Table 2.

[0056] Table 2. Stability evaluation results of liqueur in this invention. Experiment 4: Mouse Body Weight Change Experiment Male ICR mice, weighing 18-22g, were housed in a barrier environment with constant environmental parameters (temperature 22±2℃, humidity 52%±5%). The experimental animals were divided into seven groups: normal control group (CON group), model group (MOD group), negative control group (NC group), positive control group (PC group, jujube seed calming liquid), low-dose Ganoderma lucidum and Prunus cerasifera var. sarcodactylis liqueur group (GFL-L group), medium-dose Ganoderma lucidum and Prunus cerasifera var. sarcodactylis ...

[0057] Normal control group (CON group): full course of physiological saline (10mL / kg), once a day at a fixed time, for 14 consecutive days, without the establishment of para-chlorophenylalanine (PCPA) model, serving as normal physiological control.

[0058] Model group (MOD group): 35% vol light aroma baijiu was administered by gavage (10 mL / kg) once a day at a fixed time for 14 consecutive days, followed by PCPA modeling for 4 days (400 mg / kg, intraperitoneal injection).

[0059] Insomnia model group (negative control group NC group): normal saline (10mL / kg), once daily at a fixed time, for 14 consecutive days and PCPA modeling for 4 days (400mg / kg, intraperitoneal injection).

[0060] Positive control group (PC group): Jujube seed calming liquid was diluted and prepared (3 mL of jujube seed calming liquid was added with physiological saline to make up to 10 mL, and the gavage volume was 10 mL / kg). It was administered by gavage once a day at a fixed time for 14 consecutive days and PCPA modeling was carried out for 4 days (400 mg / kg, intraperitoneal injection).

[0061] GFL-L group (1.7g / kg): 0.17g / mL of liqueur was administered by gavage at a dose of 0.1mL / 10g based on mouse body weight, once daily at a fixed time, for 14 consecutive days, followed by PCPA modeling for 4 days (400mg / kg, intraperitoneal injection).

[0062] GFL-M group (3.4g / kg): 0.34g / mL of liqueur was administered by gavage at a dose of 0.1mL / 10g based on the mouse body weight, once daily at a fixed time, for 14 consecutive days, followed by PCPA modeling for 4 days (400mg / kg, intraperitoneal injection).

[0063] GFL-H group (6.8g / kg): 0.68g / mL of liqueur was administered by gavage at a dose of 0.1mL / 10g based on the mouse body weight, once daily at a fixed time, for 14 consecutive days, followed by 4 days of PCPA modeling (400mg / kg, intraperitoneal injection).

[0064] The Ganoderma lucidum and Prunus pubescens liqueur was prepared as in Example 1.

[0065] Mice were administered the medication via gavage from day 1 to day 14, and the modeling process took place from day 15 to day 18 (PCPA 300 mg / kg was injected intraperitoneally daily). Body weight was recorded for each group of mice every three days. Results of body weight changes are shown below. Figure 2 .

[0066] The experimental results show that the body mass of mice in each group using wine as solvent all showed a trend of decreasing first and then increasing. Among them, the groups with low, medium and high doses of Ganoderma lucidum Ficus hirta Vahl. fruit wine showed a faster increasing trend. After 3 days of modeling, the body mass of mice in both the model group and the negative control group decreased, and the mice showed circadian rhythm disorder, messy and dull fur, increased aggression, constant walking during the day, and weight loss caused by insomnia.

[0067] Experiment 5: Subthreshold dose hypnosis experiment of sodium pentobarbital (or sodium barbital) Grouping and administration of experimental animals were the same as those in Experiment 4. Thirty minutes after administration on the 18th day, sodium pentobarbital (45 mg / kg) was injected intraperitoneally, and sleep latency, sleep duration and sleep rate were measured; The determination method was carried out with reference to "Sodium barbital sleep latency experiment, sodium pentobarbital (or sodium barbital) subthreshold dose hypnosis experiment, prolonged sodium pentobarbital sleep time experiment in *Functional Testing and Evaluation Methods for Health Food (2023 Edition)*", statistical analysis was performed on the obtained experimental data, and the results are shown in Figure 3 .

[0068] The experimental results show that compared with the CON group, the MOD group had significantly prolonged sleep latency, significantly shortened sleep time and decreased sleep rate, indicating that the PCPA-induced insomnia model was successfully established; There was no statistically significant difference in sleep latency and sleep rate between the NC group and the MOD group. Compared with the MOD group, the PC group and each GFL dose group had significantly shortened sleep latency and significantly prolonged sleep duration, showing a dose-dependent improvement trend of GFL-L < GFL-M < GFL-H, and the effect of GFL-H was close to that of the PC group. The results show that Ganoderma lucidum Ficus hirta Vahl. fruit wine has the effect of cooperating with sodium pentobarbital to promote sleep.

[0069] Experiment 6: Open field behavior experiment Grouping and administration of experimental animals were the same as those in Experiment 4. After administration on the 18th day, the mice were placed in the center of the open field box, and the movement trajectories of the mice within 5 minutes were recorded. The results are shown in Figure 4 .

[0070] The experimental results show that the mice in the CON group mainly moved in the peripheral area, and their movement paths were relatively regular; the trajectories of the MOD group were disordered, and the number of grid crossings increased significantly, indicating that the PCPA-induced insomnia mice were in a state of hyperarousal / anxiety. The trajectories of the PC group and the GFL-H group were close to those of the CON group, and the activity range was relatively concentrated; among GFL-L, GFL-M and GFL-H, there was a dose-dependent improvement trend. The above results indicate that Ganoderma lucidum Ficus hirta Vahl. fruit wine can improve abnormal open field behavior in PCPA-induced insomnia mice, and the high dose has a better effect.

[0071] Experiment 7: Determination of 5-HT and GABA content in brain tissue The experimental animals were grouped and administered the same drugs as in Experiment 4. Mice were sacrificed on day 18 after drug administration, and brain tissue was collected. The levels of 5-HT and GABA in the brain tissue were measured using an ELISA kit. The results are as follows: Figures 5-6 As shown.

[0072] Experimental results showed that compared with the CON group, the levels of 5-HT and GABA in the brain tissue of mice in the MOD group were significantly reduced (P<0.05), suggesting that PCPA-induced decrease in the levels of 5-HT and GABA in the central nervous system of insomnia mice; there was no statistically significant difference between the NC group and the MOD group. Compared with the MOD group, the levels of 5-HT and GABA in the brain tissue of the positive control group (PC) and the medium and high dose groups of GFL were significantly increased (P<0.05, P<0.01, or P<0.001), with the GFL-H group showing the most significant effect, suggesting that the liqueur of this invention can exert a calming and sleep-inducing effect by regulating the levels of 5-HT and GABA neurotransmitters in the central nervous system.

[0073] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A red Ganoderma lucidum and five-finger peach liqueur with calming, sleep-aiding, and neuroprotective functions, characterized in that, The ingredients for this formula include: Ganoderma lucidum, five-finger peach, wolfberry, and a base wine.

2. The Ganoderma lucidum and Prunus armeniaca liqueur according to claim 1, characterized in that, The mass ratio of Ganoderma lucidum, Ficus hirta and Lycium barbarum is (10-12):(22.5-24):(7-9).

3. The Ganoderma lucidum and Prunus armeniaca liqueur according to claim 1, characterized in that, The alcohol content of the Red Lingzhi and Five-Finger Peach Liquor is 35% vol-40% vol; the Chinese medicinal materials in the Red Lingzhi and Five-Finger Peach Liquor include Red Lingzhi, Five-Finger Peach, and Goji Berry.

4. The method for preparing the Ganoderma lucidum and Prunus armeniaca liqueur according to any one of claims 1-3, characterized in that, The liqueur uses light-aroma baijiu as its base, and the base contains water extracts of traditional Chinese medicines prepared from Ganoderma lucidum, Ficus hirta, and Lycium barbarum.

5. The preparation method according to claim 4, characterized in that, The preparation method of the aqueous extract of the traditional Chinese medicine is as follows: S1. Use Ganoderma lucidum, five-finger peach and wolfberry as raw materials, wash and dry them, and soak them in water for 30-35 minutes. S2. Add water to the soaked raw materials and boil them for the first and second times. Let the combined filtrate stand and settle naturally to obtain the supernatant. S3. The supernatant is concentrated to obtain a water extract of traditional Chinese medicine.

6. The preparation method according to claim 4, characterized in that, The light-aroma baijiu is mixed with water and adjusted to the target alcohol content to obtain a blended base liquor; the blended base liquor is then mixed with the water extract of the traditional Chinese medicine to obtain a primary liqueur.

7. The preparation method according to claim 6, characterized in that, The volume ratio of the herbal extract to the light-aroma baijiu in the first decoction is 5:27; the amount of water added in the first decoction is 9-10 times the total mass of the raw materials Ganoderma lucidum, Prunus cerasifera, and Lycium barbarum; the amount of water added in the second decoction is 9-10 times the total mass of the raw materials Ganoderma lucidum, Prunus cerasifera, and Lycium barbarum.

8. The preparation method according to claim 6, characterized in that, Add flavoring agent and clarifying agent to the initial liqueur to obtain the Ganoderma lucidum and Prunus cerasifera syrup; the volume ratio of the herbal extract to the flavoring agent is (20-100):(3-9).

9. The preparation method according to claim 8, characterized in that, The flavoring agent includes glycerin, and the clarifying agent includes a chitosan solution with a concentration of 10-20 g / L, wherein the amount of chitosan solution added is 10% of the volume of the liqueur.

10. The use of the Ganoderma lucidum and Prunus armeniaca liqueur according to any one of claims 1-3 or the Ganoderma lucidum and Prunus armeniaca liqueur prepared by the preparation method according to any one of claims 4-9 in at least one of the following: 1) Prepare calming and sleep-aiding products; 2) Prepare neuroprotective products.

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