A traditional Chinese medicine formula jieyu essential oil for improving attention deficit hyperactivity disorder with anxiety

Abstract

The application discloses a traditional Chinese medicine formula Jieyu essential oil for improving attention deficit hyperactivity disorder with anxiety, and belongs to the technical field of traditional Chinese medicine essential oil. In view of the technical defects of the existing attention deficit hyperactivity disorder with anxiety treatment drugs, such as large side effects, easy to produce dependence, poor oral compliance, and lack of targeted traditional Chinese medicine compound aromatic preparation, the application provides the following technical scheme: the Jieyu essential oil is composed of aconite essential oil, polygala essential oil, curcuma essential oil, coptis essential oil, jack-in-the-pulpit essential oil, longan essential oil, Chinese date kernel essential oil, sweet-scented olive essential oil, rehmannia essential oil, salvia essential oil, camphor and borneol in a specific ratio; each single essential oil is prepared by means of pressing, supercritical carbon dioxide extraction and the like, the rectification concentration is more than 99%, and after being uniformly mixed, the Jieyu essential oil can be prepared into a nose drop, an inhalation agent or a spray. The Jieyu essential oil has the effects of improving learning and memory ability, relieving hyperactivity symptoms and resisting anxiety, and is high in safety and convenient to use.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine essential oil technology, and in particular to a traditional Chinese medicine formula for relieving depression and improving attention deficit hyperactivity disorder with anxiety. Background Technology

[0002] Attention Deficit Hyperactivity Disorder (ADHD) is a common neurodevelopmental disorder characterized by age-inappropriate inattention, hyperactivity, and impulsivity. ADHD patients often experience varying degrees of anxiety symptoms, and the co-occurrence of these symptoms further exacerbates cognitive impairment, difficulty regulating emotions, and social difficulties, severely impacting their quality of life and work / learning abilities.

[0003] Currently, the clinical treatment of ADHD with anxiety mainly involves a combination of central nervous system stimulants (such as methylphenidate hydrochloride), non-central nervous system stimulants, and anti-anxiety medications. However, long-term use of chemically synthesized drugs can easily lead to problems such as loss of appetite, insomnia, headache, cardiovascular adverse reactions, and drug dependence. Furthermore, combination therapy increases the risk of drug interactions and the incidence of adverse reactions. Traditional Chinese medicine (TCM) has unique advantages in treating ADHD and anxiety disorders, including multi-target therapy, holistic regulation, and fewer side effects, and has received widespread attention in recent years.

[0004] Herbal essential oils are volatile oily components extracted from traditional Chinese medicines. They possess pharmacological activities such as aromatic stimulation, liver soothing and mood easing, and calming the mind. They can act directly on the central nervous system through the naso-brain pathway via inhalation or nasal drops, offering rapid onset of action and convenient use. Currently, some studies have reported the effects of single-herb essential oils on improving learning and memory or reducing anxiety. Summary of the Invention

[0005] The purpose of this invention is to provide a traditional Chinese medicine formula for relieving anxiety in attention deficit hyperactivity disorder (ADHD), which can improve hyperactivity symptoms, relieve anxiety, and enhance learning and memory abilities.

[0006] To achieve the above objectives, this invention provides a traditional Chinese medicine formula for relieving anxiety in attention deficit hyperactivity disorder (ADHD), comprising the following ingredients: 4-8 parts by volume of Acorus tatarinowii essential oil, 4-8 parts by volume of Polygala tenuifolia essential oil, 4-8 parts by volume of Curcuma longa essential oil, 4-8 parts by volume of Coptis chinensis essential oil, 4-8 parts by volume of Arisaema heterophyllum essential oil, 3-7 parts by volume of Longan essential oil, 3-7 parts by volume of Ziziphus jujuba var. spinosa essential oil, 3-7 parts by volume of Albizia julibrissin essential oil, 3-7 parts by volume of Rehmannia glutinosa essential oil, 3-7 parts by volume of Salvia miltiorrhiza essential oil, 3-7 parts by weight of camphor, and 3-7 parts by weight of borneol.

[0007] The preferred formulation is as follows: 6 parts by volume of Acorus tatarinowii essential oil, 6 parts by volume of Polygala tenuifolia essential oil, 6 parts by volume of Curcuma longa essential oil, 6 parts by volume of Coptis chinensis essential oil, 6 parts by volume of Arisaema heterophyllum essential oil, 5 parts by volume of longan essential oil, 5 parts by volume of Ziziphus jujuba var. spinosa essential oil, 5 parts by volume of Albizia julibrissin essential oil, 5 parts by volume of Rehmannia glutinosa essential oil, 5 parts by volume of Salvia miltiorrhiza essential oil, 5 parts by weight of camphor, and 5 parts by weight of borneol.

[0008] Furthermore, the essential oils of Acorus tatarinowii, Polygala tenuifolia, Curcuma longa, Coptis chinensis, Arisaema heterophyllum, Longan, Ziziphus jujuba var. spinosa, Albizia julibrissin, Rehmannia glutinosa, and Salvia miltiorrhiza are extracted from the corresponding Chinese medicinal plants using methods including pressing, supercritical carbon dioxide extraction, steam distillation, soaking, chemical solvent extraction, and oil separation.

[0009] Furthermore, the distillation concentration of each essential oil is controlled to be above 99%.

[0010] This invention also provides the application of the above-mentioned traditional Chinese medicine formula for relieving depression essential oil in the preparation of a drug for improving attention deficit hyperactivity disorder with anxiety. The traditional Chinese medicine formula for relieving depression essential oil is added with medically acceptable excipients and prepared into a formulation for administration by nasal drops, inhalation or spray. Administration by nasal drops, inhalation or spray can be adapted to different usage scenarios and improve the convenience of medication.

[0011] The formulation principle of the traditional Chinese medicine formula for relieving depression essential oil in this invention is as follows: Acorus tatarinowii has a pungent and bitter taste, and is warm in nature. It enters the heart and stomach meridians and has the effects of opening the orifices and resolving phlegm, refreshing the mind and improving intelligence. It is often used for forgetfulness, insomnia, tinnitus, epilepsy and other symptoms. Modern research has found that the β-asarone and other components in the volatile oil of Acorus tatarinowii have pharmacological effects such as improving learning and memory, anti-anxiety, and sedation.

[0012] Polygala tenuifolia has a bitter and pungent taste, and is warm in nature. It enters the heart, kidney, and lung meridians. It has the effects of calming the mind and improving intelligence, harmonizing the heart and kidneys, and clearing phlegm and opening the orifices. It is often used for symptoms such as forgetfulness, insomnia, and palpitations. The volatile oil of Polygala tenuifolia has the effects of calming, anti-anxiety, and improving cognitive function.

[0013] Turmeric, with a pungent and bitter taste and a cold nature, enters the liver, heart, and lung meridians. It has the effects of promoting blood circulation and relieving pain, regulating qi and relieving depression, and clearing the heart and cooling the blood. It is often used for symptoms such as chest and rib pain, chest pain, heart pain, and insomnia. Turmeric volatile oil has the effects of anti-anxiety, anti-depression, sedation and tranquilization.

[0014] Coptis chinensis, bitter in taste and cold in nature, enters the heart, spleen, stomach, liver, gallbladder and large intestine meridians. It has the effects of clearing heat and drying dampness, purging fire and detoxifying. Modern research shows that Coptis chinensis has the effects of calming, anti-anxiety and improving cognitive function.

[0015] Arisaema has a bitter and pungent taste, and is warm in nature. It enters the lung, liver, and spleen meridians and has the effects of drying dampness and resolving phlegm, dispelling wind and relieving spasms. It is often used for symptoms such as dizziness due to wind-phlegm and epilepsy.

[0016] Longan is sweet and warm in nature, and enters the heart and spleen meridians. It has the effects of nourishing the heart and spleen, nourishing blood and calming the mind. It is often used for symptoms such as forgetfulness, insomnia, and blood deficiency with chlorosis. The active ingredients in longan have the effects of anti-anxiety and sedation.

[0017] Sour jujube seed has a sweet and sour taste, is neutral in nature, and enters the liver, gallbladder, and heart meridians. It has the effects of nourishing the heart and liver, calming the mind and soothing the nerves. It is often used for symptoms such as insomnia due to deficiency and restlessness, palpitations and excessive dreaming, and excessive sweating due to physical weakness. The saponins and flavonoids in sour jujube seed have significant sedative, anti-anxiety, and sleep-improving effects.

[0018] Albizia flowers are sweet in taste and neutral in nature. They enter the heart and liver meridians and have the effects of relieving depression and calming the mind. They are often used for symptoms such as restlessness, depression and insomnia. The volatile oil of Albizia flowers has anti-anxiety and anti-depressant effects.

[0019] Rehmannia root is sweet in taste and cold in nature. It enters the heart, liver, and kidney meridians and has the effects of clearing heat and cooling blood, nourishing yin and promoting body fluid production.

[0020] Danshen (Salvia miltiorrhiza) has a bitter taste and slightly cold nature. It enters the heart and liver meridians and has the effects of promoting blood circulation and removing blood stasis, regulating menstruation and relieving pain, and clearing the heart and relieving irritability. It is often used for symptoms such as insomnia due to heart irritability and chest pain. Tanshinone and other components in Danshen have the effects of improving learning and memory and anti-anxiety.

[0021] Camphor has a pungent taste and is hot in nature. It enters the heart and spleen meridians and has the effect of opening the orifices and dispelling foulness.

[0022] Borneol has a pungent and bitter taste, and is cool in nature. It enters the heart, spleen, and lung meridians and has the effects of opening the orifices and refreshing the mind, clearing heat and relieving pain. It is often used for symptoms such as delirium due to febrile diseases, convulsions, and phlegm syncope due to stroke. Borneol can promote the passage of other drugs across the blood-brain barrier and enhance their efficacy.

[0023] This formula uses Acorus tatarinowii and Polygala tenuifolia as the principal herbs to open the orifices, resolve phlegm, refresh the mind, improve intelligence, and calm the spirit. It uses Curcuma longa, Coptis chinensis, and Arisaema heterophyllum as the assistant herbs to regulate qi, relieve depression, clear heat and dry dampness, resolve phlegm, and dispel wind. It uses Longan aril, Ziziphus jujuba var. spinosa, Albizia julibrissin flower, Rehmannia glutinosa, and Salvia miltiorrhiza as adjuvant herbs to nourish blood, calm the mind, relieve depression, and eliminate irritability. It uses Camphor and Borneol as guiding herbs to open the orifices, dispel foul odors, and guide the medicine upwards. The entire formula works synergistically to open the orifices, refresh the mind, relieve depression, calm the spirit, resolve phlegm, and calm the spirit. It can improve hyperactivity symptoms, alleviate anxiety, and enhance learning and memory abilities.

[0024] Therefore, this invention employs the aforementioned traditional Chinese medicine formula for improving attention deficit hyperactivity disorder (ADHD) with anxiety, a relieving depression essential oil. Guided by traditional Chinese medicine theory, it rationally combines Acorus tatarinowii, Polygala tenuifolia, Curcuma longa, Coptis chinensis, Arisaema heterophyllum, Longan aril, Ziziphus jujuba var. spinosa, Albizia julibrissin, Rehmannia glutinosa, Salvia miltiorrhiza, camphor, and borneol. The components work synergistically to exert the effects of opening the orifices and refreshing the mind, relieving depression and calming the nerves, and resolving phlegm and stabilizing the will. The nasal drop administration method allows the drug to directly act on the central nervous system through the naso-brain pathway, resulting in rapid onset of action, convenient use, and high bioavailability. Furthermore, being composed of natural Chinese herbal extracts, it has low toxicity, high safety, and no dependence or significant adverse reactions associated with chemically synthesized drugs, making it convenient to use and ensuring good patient compliance.

[0025] The technical solution of the present invention will be further described in detail below through embodiments. Detailed Implementation

[0026] The technical solution of the present invention will be further described below through embodiments.

[0027] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Example 1 A traditional Chinese medicine formula for relieving anxiety in attention deficit hyperactivity disorder (ADHD) is composed of the following ingredients: 4 parts by volume of Acorus tatarinowii essential oil, 4 parts by volume of Polygala tenuifolia essential oil, 4 parts by volume of Curcuma longa essential oil, 4 parts by volume of Coptis chinensis essential oil, 4 parts by volume of Arisaema heterophyllum essential oil, 3 parts by volume of longan essential oil, 3 parts by volume of Ziziphus jujuba var. spinosa essential oil, 3 parts by volume of Albizia julibrissin essential oil, 3 parts by volume of Rehmannia glutinosa essential oil, 3 parts by volume of Salvia miltiorrhiza essential oil, 3 parts by weight of camphor, and 3 parts by weight of borneol.

[0029] The essential oils of Acorus tatarinowii, Polygala tenuifolia, Curcuma longa, Coptis chinensis, Arisaema heterophyllum, Longan aril, Ziziphus jujuba var. spinosa, Albizia julibrissin, Rehmannia glutinosa, and Salvia miltiorrhiza were all extracted from their respective medicinal plants using steam distillation. Camphor and borneol were commercially available products. The distillation concentration of each of the above essential oils was controlled to be above 99%. The components were mixed evenly according to the above volume and weight proportions to obtain the herbal formula for relieving depression, which was then sealed and refrigerated for later use.

[0030] Example 2 A traditional Chinese medicine formula for relieving anxiety in attention deficit hyperactivity disorder (ADHD) is composed of the following ingredients: 8 parts by volume of Acorus tatarinowii essential oil, 8 parts by volume of Polygala tenuifolia essential oil, 8 parts by volume of Curcuma longa essential oil, 8 parts by volume of Coptis chinensis essential oil, 8 parts by volume of Arisaema heterophyllum essential oil, 7 parts by volume of longan essential oil, 7 parts by volume of Ziziphus jujuba var. spinosa essential oil, 7 parts by volume of Albizia julibrissin essential oil, 7 parts by volume of Rehmannia glutinosa essential oil, 7 parts by volume of Salvia miltiorrhiza essential oil, 7 parts by weight of camphor, and 7 parts by weight of borneol.

[0031] The preparation method is the same as in Example 1.

[0032] Example 3 A traditional Chinese medicine formula for relieving anxiety in attention deficit hyperactivity disorder (ADHD) is composed of the following ingredients: 6 parts by volume of Acorus tatarinowii essential oil, 6 parts by volume of Polygala tenuifolia essential oil, 6 parts by volume of Curcuma longa essential oil, 6 parts by volume of Coptis chinensis essential oil, 6 parts by volume of Arisaema heterophyllum essential oil, 5 parts by volume of longan essential oil, 5 parts by volume of Ziziphus jujuba var. spinosa essential oil, 5 parts by volume of Albizia julibrissin essential oil, 5 parts by volume of Rehmannia glutinosa essential oil, 5 parts by volume of Salvia miltiorrhiza essential oil, 5 parts by weight of camphor, and 5 parts by weight of borneol.

[0033] The preparation method is the same as in Example 1.

[0034] Example 4 A traditional Chinese medicine formula for relieving anxiety in attention deficit hyperactivity disorder (ADHD) is composed of the following ingredients: 5 parts by volume of Acorus tatarinowii essential oil, 5 parts by volume of Polygala tenuifolia essential oil, 5 parts by volume of Curcuma longa essential oil, 5 parts by volume of Coptis chinensis essential oil, 5 parts by volume of Arisaema heterophyllum essential oil, 4 parts by volume of longan essential oil, 4 parts by volume of Ziziphus jujuba var. spinosa essential oil, 4 parts by volume of Albizia julibrissin essential oil, 4 parts by volume of Rehmannia glutinosa essential oil, 4 parts by volume of Salvia miltiorrhiza essential oil, 4 parts by weight of camphor, and 4 parts by weight of borneol.

[0035] The preparation method is the same as in Example 1.

[0036] Experimental Example 1 1. Experimental Materials 1.1 Laboratory Animals Eighty-four SPF-grade KM mice, half male and half female, aged 3-4 weeks, were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd., license number SCXK (Lu) 20220006, animal qualification certificate number: No. 370726231100479323. The ambient temperature was (22±2)℃, the light cycle in the animal room was 12h, and the mice had free access to food and water. They were used in this experiment after 7 days of acclimatization.

[0037] 1.2 Experimental Apparatus Open field test chamber (Chengdu Taimeng Software Co., Ltd., OFT-100), mouse spontaneous activity instrument (Chengdu Taimeng Software Co., Ltd., ZZ-6), elevated cross maze test instrument (Chengdu Taimeng Software Co., Ltd., PMT-200), Morris water maze (Chengdu Taimeng Software Co., Ltd., WMT-100), grinding instrument (Shanghai Jingxin Industrial Development Co., Ltd., JXFSTPRP-48).

[0038] 1.3 Experimental Reagents Scopolamine hydrobromide injection (Jilin Huamu Animal Health Products Co., Ltd., batch number 20230324), Jingling oral solution (Liaoning Dongfangren Pharmaceutical Co., Ltd., batch number 20220909), methylphenidate hydrochloride sustained-release tablets (Janssen-Cilag Manufacturing, batch number 2HE481), sodium pentobarbital (Sinopharm Chemical Reagent Co., Ltd., batch number 690201100).

[0039] 1.4 Experimental Drugs The traditional Chinese medicine formula for relieving depression essential oil of this invention adopts the formula of Example 3. After being mixed evenly, medically acceptable excipients are added to prepare a preparation for administration by nasal drops.

[0040] 2 Experimental Methods 2.1 Animal Model Preparation After one week of laboratory acclimatization, 14 mice were randomly selected as the blank control group, while the remaining mice were used to establish an animal model of attention deficit hyperactivity disorder (ADHD) with anxiety. Except for the blank control group, all other groups received daily intraperitoneal injections of scopolamine hydrobromide at a dose of 3 mg / kg. On the first day, the mice were restrained in 50 mL centrifuge tubes for 2 hours, with the restraint time increasing by 2 hours daily until reaching 8 hours, for 7 consecutive days. The blank control group received the same daily intraperitoneal injection of physiological saline.

[0041] 2.2 Grouping and Administration Methods The animals were randomly divided into groups of 14 each according to their weight. The groups were: blank group, model group, high-dose Jieyu essential oil group, low-dose Jieyu essential oil group, Jingling oral liquid group, and methylphenidate hydrochloride tablet group.

[0042] Jingling oral solution group (2.496 mL / kg) and methylphenidate hydrochloride tablet group (2.262 mg / kg) were administered by gavage daily; the high-dose Jieyu essential oil group was administered 10 μL / animal by nasal drops daily; the low-dose Jieyu essential oil group was administered 5 μL / animal by nasal drops daily; the model group and the blank group were administered an equal volume of physiological saline by gavage daily. Administration continued for 8 days.

[0043] 2.3 Morris Water Maze Experiment Following the last dose, all mice underwent the Morris water maze test, which included a navigation and spatial exploration experiment. The Morris water maze laboratory tests animals' spatial location and spatial orientation learning and memory abilities by forcing them to swim and learn to find platforms hidden in the water. Rats are natural swimmers and instinctively seek out resting places in the water. The animals use different markers affixed to the pool walls to spatially orient themselves, aiming to successfully find the hidden platforms and ultimately escape from the water. This method can be used to study working memory and spatial orientation abilities.

[0044] (1) Positioning and cruising experiment: One day before the experiment, the mice were allowed to swim freely in the pool for 2 minutes to adapt to the environment. Then, the positioning and cruising experiment was carried out for 4 consecutive days. Each day, the mice were randomly placed into the pool from 4 different entry points, and the time it took for them to find the hidden platform (i.e., the escape latency period) was recorded. If the mice did not find the platform within 90 seconds, they were guided to the platform and stayed there for 20 seconds. The escape latency period at this time was recorded as 90 seconds.

[0045] (2) Spatial exploration experiment: On the 5th day, the platform was removed and a spatial exploration experiment was conducted. The time the mice spent in the original platform quadrant (i.e., the time spent in the target quadrant), the number of times they crossed the target platform, and their swimming speed were recorded to assess the mice's spatial memory ability.

[0046] 2.4 Mouse Spontaneous Activity Experiment Mice were placed in a spontaneous activity device and allowed to acclimatize to the experimental environment for 15 minutes. Their spontaneous activity was then tested over a 10-minute period, recording the number of movements and standing positions. The mouse spontaneous activity device can detect the number of movements and standing positions of mice within a specified time, and can reflect the degree of hyperactivity in mice to some extent.

[0047] 2.5 Open Field Experiment An open field test, also known as an open box test, is used to assess spontaneous activity and exploratory behavior in animals. The test evaluates the autonomous behavior and stress levels of experimental animals in unfamiliar environments. The test utilizes a 25-square grid, with the central area consisting of 9 squares and the perimeter and corner areas consisting of 16 squares. Animals are placed in the open field 5 minutes beforehand to acclimatize, and their activity area, time, and distance are recorded within a specified 5-minute timeframe. The test is conducted in a quiet environment. After completion, the test chamber is cleaned and wiped with 75% alcohol.

[0048] 2.6 Elevated Cross Maze Experiment The open-arm and closed-arm areas were located in the software. Animals were allowed 5 minutes to adapt to the environment before the experiment began. The distance, time, and number of entries into the open-arm and closed-arm areas were recorded within 5 minutes. The elevated cross maze is a laboratory experiment for evaluating anxiety responses in rodents, providing a direct reflection of the mouse's conditioned response. It consists of two open arms and two closed arms, arranged in a cross shape, at a certain height above the ground. The principle is that when faced with a new object ("open arm"), the mouse exhibits curiosity to explore, but also has a natural tendency to avoid darkness ("close arm"). This conflict between exploration and avoidance creates anxiety. The height of the maze above the ground causes the animal to feel fear and unease. The anxiety behavior of the mouse is evaluated by comparing the time spent and distance traveled in the open and closed arms. The elevated cross maze can establish an anxiety stress model and is an internationally recognized classic method for measuring anxiety responses. During the test, anxious animals showed a significant decrease in the time spent in the open arms and the number of times they entered them; the total number of entries into the open and closed arms can reflect the mouse's activity level to some extent.

[0049] 3. Statistical processing methods The data analysis software package (GraphPad Prism 9.0.0) was used to perform statistical analysis and processing on the measurement data. Each group of data was presented as mean ± standard deviation (x ± s). One-way ANOVA was used to compare the experimental groups. The LSD method is applicable when the variances are homogeneous (P>0.05), while the Games-Howell method is applicable when the variances are unequal (P<0.05).

[0050] 4 Experimental Results 4.1 Results of the Morris water maze experiment 4.1.1 Time for mice to first reach the effective area over 4 consecutive days Table 1 shows that during the training process, the time taken by the control group mice to find the escape platform decreased with the increase of training days and was significantly lower than that of the model group mice, indicating that the spatial memory ability of the model group mice was impaired after modeling. After treatment with Jieyu essential oil, the time taken by each group of mice to find the platform decreased and was significantly lower than that of the model group, indicating that Jieyu essential oil improved the spatial memory and learning ability of mice to varying degrees, with the high-dose group showing the most significant effect.

[0051] Table 1. Time of first arrival of mice in the effective area over 4 consecutive days

[0052] Note: Compared to day one, # P <0.05, ## P<0.01; compared with the model group, * P <0.05, ** P <0.01 4.1.2 Time and number of shuttles for the first time the mice reached the effective area on the fifth day Table 2 shows that after four days of training, the time taken for the model group mice to first reach the effective area was significantly longer than that of the control group mice, indicating that the spatial memory and learning abilities of the model group mice were severely impaired. After treatment with the antidepressant essential oil, the time taken for the mice to first reach the effective area was significantly shortened in all groups, with the high-dose antidepressant essential oil group showing the most significant effect. The number of times the model group mice shuttled through the effective area was significantly reduced, indicating that the mice had memory impairment, while the number of shuttles in all groups was significantly increased after the essential oil treatment.

[0053] Table 2. Time and number of shuttles for the first time reaching the effective area on day 5 (x±s)

[0054] Note: Compared to the model group, ** P <0.01. 4.2 Results of the mouse spontaneous activity experiment Table 3 shows that the number of activities and standing times of the model group mice within the specified time were significantly higher than those of the control group, indicating that the model group mice were overactive. After administration of the Jieyu essential oil, the hyperactivity of the mice was alleviated to varying degrees, with the high-dose Jieyu essential oil group showing the most significant effect, in which the number of activities and standing times of the mice was significantly reduced compared to the model group.

[0055] Table 3 Number of spontaneous movements and number of standing up in mice

[0056] Note: Compared to the model group, * P <0.05, ** P <0.01 4.3 Results of the open field experiment Table 4 shows that the activity level of the model group mice in the open field was significantly increased, reflecting hyperactivity. After treatment with the antidepressant essential oil, the activity level of the mice in the open field decreased, with the high-dose group showing the most significant effect. The model group mice were more active in the edges and corners of the open field, and their activity time was significantly longer than that of the control group, indicating that the anxiety level of the model group mice was higher, proving that the anxiety factors were successfully replicated during the experimental modeling process. After treatment with the antidepressant essential oil, the anxiety levels of the mice decreased to varying degrees.

[0057] Table 4. Movement distance and time spent moving to edges and corners of mice in an open field (x±s)

[0058] Note: Compared to the model group, * P <0.05, ** P <0.01 4.4 Results of the elevated cross-maze experiment Table 5 shows that compared with the control group, the model group mice exhibited significantly reduced dwell time in the open arm, fewer entries into the open arm, and greater movement distance within the open arm, indicating a higher anxiety level in the model group mice. This corroborates the conclusion from the open field test that the model group mice spent more time in corners and edges, proving the success of incorporating anxiety factors during modeling. After treatment with the anti-anxiety essential oil, the dwell time in the open arm, movement distance, and number of entries into the open arm increased to varying degrees in all groups of mice, demonstrating that the anti-anxiety essential oil of this invention can alleviate anxiety behavior in animals to varying degrees, with the high-dose group showing the best effect.

[0059] Table 5. Mouse movement time, percentage of movement distance, and percentage of entry attempts during open-arm movement (x±s)

[0060] Note: Compared to the model group, * P <0.05, ** P <0.01 This experiment used intraperitoneal injection of scopolamine combined with restraint to replicate an animal model of attention deficit hyperactivity disorder (ADHD) with anxiety. Behavioral testing results showed that all mice in the model group exhibited symptoms such as hyperactivity, learning and memory deficits, and elevated anxiety levels after modeling, indicating successful model replication.

[0061] The therapeutic essential oil of this invention was administered via nasal drops to mice with attention deficit hyperactivity disorder (ADHD) and anxiety. Results showed that the therapeutic essential oil had a significant therapeutic effect on the model mice, particularly in improving their learning and spatial memory abilities. In the Morris water maze test, the mice in the therapeutic essential oil group, after training, were able to quickly locate the escape platform. After the escape platform was removed, the mice repeatedly wandered and traversed the original platform location, indicating strong spatial memory in this group. Open field and elevated cross maze tests demonstrated that the therapeutic essential oil has good anti-anxiety effects, with the high-dose group showing the best results. After nasal drop treatment, the mice in the therapeutic essential oil group showed increased central area movement time in the open field test, and significantly higher dwell time, movement distance, and number of times entering the open arm in the elevated cross maze test compared to the model group. Furthermore, after administration of the therapeutic essential oil, the number of activities and standing times detected by the spontaneous movement instrument were significantly reduced, and the activity time and movement distance in the open field were also significantly lower than those in the model group. The above results corroborate each other, indicating that the anti-depression essential oil of the present invention can effectively alleviate hyperactivity symptoms, anxiety behavior and learning and memory impairment in mice, and has a significant improving effect on attention deficit hyperactivity disorder with anxiety.

[0062] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A traditional Chinese medicine formula for relieving anxiety in attention deficit hyperactivity disorder (ADHD), characterized in that: It is composed of the following ingredients: 4-8 parts by volume of Acorus tatarinowii essential oil, 4-8 parts by volume of Polygala tenuifolia essential oil, 4-8 parts by volume of Curcuma longa essential oil, 4-8 parts by volume of Coptis chinensis essential oil, 4-8 parts by volume of Arisaema heterophyllum essential oil, 3-7 parts by volume of Longan essential oil, 3-7 parts by volume of Ziziphus jujuba var. spinosa essential oil, 3-7 parts by volume of Albizia julibrissin flower essential oil, 3-7 parts by volume of Rehmannia glutinosa essential oil, 3-7 parts by volume of Salvia miltiorrhiza essential oil, 3-7 parts by weight of camphor, and 3-7 parts by weight of borneol.

2. The traditional Chinese medicine formula for relieving anxiety in attention deficit hyperactivity disorder (ADHD) according to claim 1, characterized in that, It is composed of the following ingredients: 6 parts by volume of Acorus tatarinowii essential oil, 6 parts by volume of Polygala tenuifolia essential oil, 6 parts by volume of Curcuma longa essential oil, 6 parts by volume of Coptis chinensis essential oil, 6 parts by volume of Arisaema heterophyllum essential oil, 5 parts by volume of Longan essential oil, 5 parts by volume of Ziziphus jujuba var. spinosa essential oil, 5 parts by volume of Albizia julibrissin essential oil, 5 parts by volume of Rehmannia glutinosa essential oil, 5 parts by volume of Salvia miltiorrhiza essential oil, 5 parts by weight of camphor, and 5 parts by weight of borneol.

3. The traditional Chinese medicine formula for relieving anxiety in patients with attention deficit hyperactivity disorder (ADHD) according to claim 1 or 2, characterized in that, The essential oils of Acorus tatarinowii, Polygala tenuifolia, Curcuma longa, Coptis chinensis, Arisaema heterophyllum, Longan, Ziziphus jujuba var. spinosa, Albizia julibrissin, Rehmannia glutinosa, and Salvia miltiorrhiza are made from the corresponding Chinese medicinal plants. The extraction methods include pressing extraction, supercritical carbon dioxide extraction, steam distillation extraction, soaking method, chemical solvent extraction method, and oil separation method.

4. The traditional Chinese medicine formula for relieving anxiety in attention deficit hyperactivity disorder (ADHD) according to claim 3, characterized in that, The distillation concentration of each essential oil is controlled to be above 99%.

5. The application of the traditional Chinese medicine formula for relieving depression essential oil according to claim 1 or 2 in the preparation of a drug for improving attention deficit hyperactivity disorder with anxiety, characterized in that, The herbal compound essential oil is prepared by adding medically acceptable excipients to form a formulation for administration via nasal drops, inhalation, or spray.

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