A traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction and a preparation method thereof

Abstract

The application belongs to the technical field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for preventing and treating heart infarction and / or brain infarction and a preparation method thereof, which comprises the following raw materials in parts by mass: 25-30 parts of salvia miltiorrhiza, 15-22 parts of panax notoginseng, 17-25 parts of rubus suavissimus, 15-18 parts of apocynum venetum leaves, 8-12 parts of curcuma zedoaria, and 5-8 parts of platycodon grandiflorum. The salvia miltiorrhiza, panax notoginseng, rubus suavissimus, apocynum venetum leaves, curcuma zedoaria and platycodon grandiflorum are combined according to specific proportions, the salvia miltiorrhiza and panax notoginseng are used as monarch drugs, the apocynum venetum leaves and curcuma zedoaria are used as minister drugs, the curcuma zedoaria is used as an auxiliary drug, and the platycodon grandiflorum is used as a guide drug. The core pathogenesis of heart infarction and brain infarction is blood stasis and collateral obstruction, and the monarch-minister-auxiliary-guide system is constructed. The whole prescription is rigorous, has the effects of activating blood and dissolving thrombus, removing plaque and protecting blood vessels, regulating blood pressure and resisting inflammation, and promoting qi and dredging collaterals, can simultaneously intervene in the key links of the onset and recovery of heart infarction and brain infarction, can effectively reduce the area of cerebral infarction and the area of heart infarction, and has excellent effects of treating brain infarction and heart infarction.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, specifically to a traditional Chinese medicine composition for the prevention and treatment of myocardial infarction and / or cerebral infarction and its preparation method. Background Technology

[0002] Myocardial infarction and cerebral infarction are highly prevalent, deadly, and disabling acute and critical cardiovascular and cerebrovascular diseases that seriously threaten the health and quality of life of the population. Their core pathological processes include the progression of atherosclerosis, vascular stenosis or occlusion, hypercoagulable state of the blood, and thrombosis. They are often accompanied by multiple pathological changes such as vascular endothelial damage, abnormal blood pressure, local inflammation and edema, and microcirculatory disturbances, leading to rapid disease progression, severe sequelae, and a persistently high recurrence rate.

[0003] Currently, clinical treatment for myocardial infarction and cerebral infarction mainly relies on Western medicine drugs such as anticoagulants, antiplatelet drugs, vasodilators, and antihypertensive drugs, as well as interventional thrombectomy and stent implantation for revascularization. However, the above-mentioned approaches have significant technical limitations and clinical shortcomings: long-term use of Western medicine can easily lead to bleeding tendencies, liver and kidney damage, and drug resistance, and its targets are relatively singular. Although it can improve coagulation function or blood pressure indicators, it is difficult to simultaneously repair damaged vascular endothelium, ablate old atherosclerotic plaques, or fundamentally improve the overall blood stasis state of the body; although interventional surgery has a clear effect on acute large vessel occlusion, its effect on improving cerebral microvascular embolism, old thrombi, postoperative neurological deficits, and myocardial injury sequelae is limited, and it cannot effectively inhibit the regeneration of atherosclerotic plaques and thrombus recurrence.

[0004] Therefore, this application is submitted. Summary of the Invention

[0005] This invention provides a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction, and its preparation method. The traditional Chinese medicine composition described in this application can effectively reduce the area of ​​cerebral infarction and myocardial infarction, and has excellent therapeutic effects on cerebral infarction and myocardial infarction.

[0006] The present invention solves its technical problem by adopting the following technical solution: A traditional Chinese medicine composition for the prevention and treatment of myocardial infarction and / or cerebral infarction comprises the following raw materials in parts by weight: 25-30 parts of Salvia miltiorrhiza, 15-22 parts of Panax notoginseng, 17-25 parts of Lysimachia christinae, 15-18 parts of Apocynum venetum leaf, 8-12 parts of Curcuma zedoaria, and 5-8 parts of Platycodon grandiflorus.

[0007] As an implementation scheme of this application, the preparation raw materials include the following parts by weight: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of Lysimachia christinae, 16 parts of Apocynum venetum leaf, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0008] This application also provides a method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction, comprising the following steps: (1) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae, supercritical extraction was performed to obtain extract and residue; the extract was dried to obtain the first extract; the residue was extracted with water, filtered and dried to obtain the second extract. (2) After crushing Panax notoginseng and Apocynum venetum leaves, add them to water, then add cellulase, pectinase and β-glucosidase, hydrolyze, inactivate enzymes, dry, and obtain the third extract; (3) After crushing the platycodon root, extract it with water, filter it, and dry it to obtain the fourth extract; (4) The first extract, second extract, third extract and fourth extract are mixed evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0009] As an embodiment of this application, the supercritical extraction uses CO2 as the extractant, 70-85 wt% ethanol solution as the entrainer, and the CO2 flow rate is 50-80 mL / min.

[0010] As an embodiment of this application, the supercritical extraction temperature is 35~42℃, the pressure is 28~32MPa, and the time is 70~100min.

[0011] As an embodiment of this application, the mass ratio of the extract residue to water is 1:(8~20). Step (1) Water extraction temperature is 65~80℃, time is 1~4h.

[0012] As an embodiment of this application, the total mass of Panax notoginseng and Apocynum venetum leaves, and the mass ratio of cellulase, pectinase, β-glucosidase, and water is 1:(0.005~0.008):(0.004~0.008):(0.003~0.005):(8~20).

[0013] As an embodiment of this application, the enzymatic hydrolysis temperature is 44~50℃ and the time is 1~4h.

[0014] As an embodiment of this application, the enzyme inactivation temperature is 85~95℃ and the time is 5~20min.

[0015] As an implementation scheme of this application, in step (3), the mass ratio of Platycodon grandiflorus to water is 1:(5~20), the water extraction temperature is 60~90℃, and the time is 1~6h.

[0016] The beneficial effects of this invention are as follows: This application combines Salvia miltiorrhiza, Panax notoginseng, Lysimachia christinae, Apocynum venetum leaf, Curcuma zedoaria, and Platycodon grandiflorus in a specific ratio, with Salvia miltiorrhiza and Panax notoginseng as the principal herbs, Apocynum venetum leaf and Curcuma zedoaria as the assistant herbs, Curcuma zedoaria as the adjuvant herb, and Platycodon grandiflorus as the guiding herb. Targeting the core pathogenesis of myocardial infarction and cerebral infarction, such as blood stasis, qi stagnation, arteriosclerosis, and unstable blood pressure, it constructs a principal-assistant-adjuvant-guide combination system. The entire formula is rigorously combined and has the effects of promoting blood circulation and dissolving thrombi, eliminating spots and protecting blood vessels, regulating blood pressure and reducing inflammation, and promoting qi circulation and unblocking collaterals. It can simultaneously intervene in the key links of the onset and recovery of myocardial infarction and cerebral infarction, effectively reducing the area of ​​cerebral infarction and myocardial infarction, and has excellent therapeutic effects on cerebral infarction and myocardial infarction. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0019] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0020] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0021] Unless otherwise specified, all reagents or instruments used in this invention are commercially available conventional products. Unless otherwise specified, the raw materials used in each comparative example and the parallel experiments of each embodiment are the same commercially available products.

[0022] This application provides a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction, comprising the following raw materials in parts by weight: 25-30 parts of Salvia miltiorrhiza, 15-22 parts of Panax notoginseng, 17-25 parts of Lysimachia christinae, 15-18 parts of Apocynum venetum leaf, 8-12 parts of Curcuma zedoaria, and 5-8 parts of Platycodon grandiflorus.

[0023] The dosage of Danshen is 25 to 30 parts, for example, it can be 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, or any two of these values.

[0024] The dosage of Panax notoginseng is 15 to 22 parts, for example, it can be 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, or any two of these values.

[0025] The dosage of red root grass is 17 to 25 parts, for example, it can be 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, or any two of these values.

[0026] The dosage of Apocynum venetum leaves is 15 to 18 parts, for example, 15 parts, 16 parts, 17 parts, 18 parts, or any two of these values.

[0027] The dosage of Curcuma zedoaria is 8 to 12 parts, for example, it can be 8 parts, 9 parts, 11 parts, 11 parts, 12 parts or any two of these values.

[0028] The dosage of Platycodon grandiflorus is 5 to 8 parts, for example, it can be 5 parts, 6 parts, 7 parts, 8 parts or any two of these values.

[0029] Among them, Danshen has the effects of removing blood stasis and relieving pain, promoting blood circulation and regulating menstruation. It can dilate coronary arteries and cerebral microvessels, increase coronary blood flow, and improve myocardial ischemia and hypoxia; inhibit platelet aggregation, reduce blood viscosity, and reduce coronary thrombosis; reduce myocardial oxidative stress damage, reduce the area of ​​myocardial infarction necrosis, and relieve chest tightness, chest pain, and palpitations. At the same time, it can clear free radicals in the brain, reduce cerebral cell edema and apoptosis caused by ischemia-reperfusion, and prevent the continuous enlargement of cerebral artery plaques.

[0030] Among them, Panax notoginseng has the effects of dispersing blood stasis, stopping bleeding, reducing swelling and relieving pain. It can exert a comprehensive effect of anti-platelet aggregation, anti-inflammatory and antioxidant, anti-apoptosis, improving endothelial function and promoting angiogenesis by regulating multiple signaling pathways such as PI3K / Akt, MAPK and NF-κB. At the same time, Panax notoginseng can also dilate blood vessels, increase blood flow and improve blood circulation.

[0031] Among them, red root grass has the effects of clearing heat and dampness, promoting blood circulation and removing blood stasis, and is specifically used for cardiovascular and cerebrovascular diseases with blood stasis and blood heat. It can improve microcirculation, protect ischemic myocardium, dissolve thrombi in the infarct site, improve blood viscosity, and promote functional recovery of the cerebral infarction site.

[0032] Among them, Apocynum venetum leaves have the effects of calming the liver and suppressing yang, clearing heat and promoting diuresis, lowering blood pressure and unblocking collaterals. They can gradually lower peripheral blood pressure, reduce the heart's pumping load, and reduce the impact damage of high pressure on coronary arteries; relieve chest tightness, shortness of breath and lower limb edema associated with heart failure; inhibit sympathetic nerve excitation, reduce the risk of acute myocardial infarction induced by sudden rise in blood pressure, and reduce plaque detachment caused by blood pressure fluctuations.

[0033] Among them, Curcuma zedoaria has the effects of breaking up blood stasis, promoting qi circulation, eliminating masses and dispersing nodules. It can break up old stasis and atherosclerotic masses in the coronary arteries, and clear severely narrowed coronary arteries; it can also inhibit vascular fibrosis and prevent the coronary arteries from hardening and narrowing. At the same time, it can clear severely blocked cerebral blood vessels, improve limb stiffness and limited mobility caused by qi and blood stagnation after cerebral infarction, and inhibit the formation of new thrombi.

[0034] Among them, Platycodon grandiflorus has the effects of promoting lung function and regulating qi, carrying medicine upward, and promoting blood circulation. It can resolve qi stagnation and blood stasis, relieve chest tightness and shortness of breath; promote qi and blood circulation, clear the obstruction of qi and blood in the coronary arteries in the chest, improve chest pain and shortness of breath, and carry the blood-activating medicines upward to the head, face and brain, guiding other components directly to the intracranial lesions; promote qi circulation, clear the meridians of the brain, and help improve the circulation of qi and blood throughout the body.

[0035] This application creatively combines Salvia miltiorrhiza, Panax notoginseng, Lysimachia christinae, Apocynum venetum leaf, Curcuma zedoaria, and Platycodon grandiflorus in a specific ratio, with Salvia miltiorrhiza and Panax notoginseng as the principal herbs, Apocynum venetum leaf and Curcuma zedoaria as the assistant herbs, Curcuma zedoaria as the adjuvant herb, and Platycodon grandiflorus as the guiding herb. Targeting the core pathogenesis of myocardial infarction and cerebral infarction, including blood stasis, qi stagnation, arteriosclerosis, and unstable blood pressure, it constructs a principal-assistant-adjuvant-guide system. The entire formula is rigorously formulated, possessing the effects of promoting blood circulation and dissolving thrombi, eliminating spots and protecting blood vessels, regulating blood pressure and reducing inflammation, and promoting qi circulation and unblocking collaterals. It can simultaneously intervene in the key aspects of the onset and recovery of myocardial infarction and cerebral infarction, effectively reducing the area of ​​cerebral infarction and myocardial infarction, and has excellent therapeutic effects on cerebral infarction and myocardial infarction.

[0036] In some embodiments, the preparation materials include the following parts by weight: 28 parts Salvia miltiorrhiza, 18 parts Panax notoginseng, 22 parts Lysimachia christinae, 16 parts Apocynum venetum leaf, 10 parts Curcuma zedoaria, and 6 parts Platycodon grandiflorus.

[0037] In some embodiments, the traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction may also be selected by adding at least one of green tea, licorice and rose petals as needed.

[0038] An embodiment of this application also provides a method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction, comprising the following steps: (1) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae, supercritical extraction was performed to obtain extract and residue; the extract was dried to obtain the first extract; the residue was extracted with water, filtered and dried to obtain the second extract. (2) After crushing Panax notoginseng and Apocynum venetum leaves, add them to water, then add cellulase, pectinase and β-glucosidase, hydrolyze, inactivate enzymes, dry, and obtain the third extract; (3) After crushing the platycodon root, extract it with water, filter it, and dry it to obtain the fourth extract; (4) The first extract, second extract, third extract and fourth extract are mixed evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0039] In some embodiments, the supercritical extraction uses CO2 as the extractant, 70-85 wt% ethanol solution as the entrainer, and the CO2 flow rate is 50-80 mL / min.

[0040] In some embodiments, the supercritical extraction temperature is 35~42°C, the pressure is 28~32 MPa, and the time is 70~100 min.

[0041] In some embodiments, the mass ratio of the extract residue to water is 1:(8~20); Step (1) Water extraction temperature is 65~80℃, time is 1~4h.

[0042] In some embodiments, the total mass of Panax notoginseng and Apocynum venetum leaves is in the mass ratio of cellulase, pectinase, β-glucosidase and water as 1:(0.005~0.008):(0.004~0.008):(0.003~0.005):(8~20).

[0043] In some embodiments, the enzymatic hydrolysis is performed at a temperature of 44-50°C for 1-4 hours.

[0044] In some embodiments, the enzyme inactivation temperature is 85-95°C and the time is 5-20 minutes.

[0045] In some embodiments, in step (3), the mass ratio of Platycodon grandiflorus to water is 1:(5~20), the water extraction temperature is 60~90℃, and the time is 1~6h.

[0046] The present application is further illustrated below with specific embodiments:

[0047] Example 1 A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of red root grass, 16 parts of Apocynum venetum leaf, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0048] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0049] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0050] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0051] (4) After crushing the platycodon root, add 10 times the mass of water, extract at 75℃ for 4 hours, filter, dry, and obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0052] Example 2 A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 25 parts Salvia miltiorrhiza, 22 parts Panax notoginseng, 25 parts Lysimachia christinae, 15 parts Apocynum venetum leaf, 8 parts Curcuma zedoaria, and 5 parts Platycodon grandiflorus.

[0053] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0054] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0055] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0056] (4) After crushing the platycodon root, add 10 times the mass of water, extract at 75℃ for 4 hours, filter, dry, and obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0057] Example 3 A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 30 parts of Salvia miltiorrhiza, 15 parts of Panax notoginseng, 17 parts of red root grass, 18 parts of Apocynum venetum leaf, 12 parts of Curcuma zedoaria, and 8 parts of Platycodon grandiflorus.

[0058] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0059] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0060] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0061] (4) After crushing the platycodon root, add 10 times the mass of water, extract at 75℃ for 4 hours, filter, dry, and obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0062] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the proportions of Salvia miltiorrhiza, Panax notoginseng, Lysimachia christinae, Apocynum venetum leaf, Curcuma zedoaria, and Platycodon grandiflorus are different, while all other proportions are the same.

[0063] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 20 parts of Salvia miltiorrhiza, 28 parts of Panax notoginseng, 30 parts of red root grass, 12 parts of Apocynum venetum leaf, 6 parts of Curcuma zedoaria, and 4 parts of Platycodon grandiflorus.

[0064] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0065] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0066] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0067] (4) After crushing the platycodon root, add 10 times the weight of water, extract at 75°C for 4 hours, filter, and dry to obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0068] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the proportions of Salvia miltiorrhiza, Panax notoginseng, Lysimachia christinae, Apocynum venetum leaf, Curcuma zedoaria, and Platycodon grandiflorus are different, while the rest are the same.

[0069] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 36 parts of Salvia miltiorrhiza, 6 parts of Panax notoginseng, 8 parts of red root grass, 22 parts of Apocynum venetum leaf, 16 parts of Curcuma zedoaria, and 12 parts of Platycodon grandiflorus.

[0070] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0071] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0072] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0073] (4) After crushing the platycodon root, add 10 times the weight of water, extract at 75°C for 4 hours, filter, and dry to obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0074] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain Salvia miltiorrhiza, but everything else is the same.

[0075] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 18 parts Panax notoginseng, 22 parts Red Root Grass, 16 parts Apocynum venetum leaves, 10 parts Curcuma zedoaria, and 6 parts Platycodon grandiflorus.

[0076] (2) After pulverizing Curcuma zedoaria and Rhododendron simsii to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0077] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0078] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0079] (4) After crushing the platycodon root, add 10 times the mass of water, extract at 75℃ for 4 hours, filter, dry, and obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0080] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain Panax notoginseng, but everything else is the same.

[0081] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 22 parts of Lysimachia christinae, 16 parts of Apocynum venetum leaf, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0082] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0083] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0084] (3) After crushing the leaves of Apocynum venetum to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the mass ratio of Apocynum venetum leaves, cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0085] (4) After crushing the platycodon root, add 10 times the mass of water, extract at 75℃ for 4 hours, filter, dry, and obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0086] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain red root grass, but everything else is the same.

[0087] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 16 parts of Apocynum venetum leaf, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0088] (2) After pulverizing Salvia miltiorrhiza and Curcuma zedoaria to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0089] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0090] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0091] (4) After crushing the platycodon root, add 10 times the weight of water, extract at 75°C for 4 hours, filter, and dry to obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0092] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not include Apocynum venetum leaves, but everything else is the same.

[0093] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of Lysimachia christinae, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0094] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0095] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0096] (3) After crushing Panax notoginseng to 60 mesh, add it to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze it at 46℃ for 2.5h, then inactivate the enzyme at 90℃ for 10min, filter, dry, and obtain the third extract; wherein, the mass ratio of Panax notoginseng, cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0097] (4) After crushing the platycodon root, add 10 times the weight of water, extract at 75°C for 4 hours, filter, and dry to obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0098] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 does not contain Curcuma zedoaria, but everything else is the same.

[0099] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of red root grass, 16 parts of Apocynum venetum leaf, and 6 parts of Platycodon grandiflorus.

[0100] (2) After crushing the Salvia miltiorrhiza and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain the extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, the supercritical extraction temperature is 40℃, the pressure is 30MPa, and the time is 80min.

[0101] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0102] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0103] (4) After crushing the platycodon root, add 10 times the weight of water, extract at 75°C for 4 hours, filter, and dry to obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0104] Comparative Example 8 The difference between Comparative Example 8 and Example 1 is that Comparative Example 8 does not contain Platycodon grandiflorus, but everything else is the same.

[0105] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of red root grass, 16 parts of Apocynum venetum leaf, and 10 parts of Curcuma zedoaria.

[0106] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0107] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0108] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0109] (4) Mix the first extract, second extract and third extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0110] Comparative Example 9 The difference between Comparative Example 9 and Example 1 is that Comparative Example 9 uses an equal amount of Ligusticum striatum to replace Panax notoginseng, while everything else is the same.

[0111] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Ligusticum chuanxiong, 22 parts of Lysimachia christinae, 16 parts of Apocynum venetum leaf, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0112] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0113] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0114] (3) After crushing the leaves of Ligusticum chuanxiong and Apocynum venetum to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Ligusticum chuanxiong and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0115] (4) After crushing the platycodon root, add 10 times the weight of water, extract at 75°C for 4 hours, filter, and dry to obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0116] Comparative Example 10 The difference between Comparative Example 10 and Example 1 is that Comparative Example 10 uses an equal amount of jujube seed instead of red root grass, while everything else is the same.

[0117] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of Ziziphus jujuba var. spinosa, 16 parts of Apocynum venetum leaf, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0118] (2) After grinding Salvia miltiorrhiza, Curcuma zedoaria and Ziziphus jujuba seeds to 60 mesh, they were added to the extraction vessel for supercritical extraction to obtain extract and raffinate; the extract was dried to obtain the first extract; wherein, the supercritical extraction used CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate was 60mL / min, the supercritical extraction temperature was 40℃, the pressure was 30MPa, and the time was 80min.

[0119] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0120] (3) After crushing Panax notoginseng and Apocynum venetum leaves to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Apocynum venetum leaves and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0121] (4) After crushing the platycodon root, add 10 times the weight of water, extract at 75°C for 4 hours, filter, and dry to obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0122] Comparative Example 11 The difference between Comparative Example 11 and Example 1 is that Comparative Example 11 uses an equal amount of Astragalus membranaceus to replace the amount of Apocynum venetum leaves, while everything else is the same.

[0123] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of Lysimachia christinae, 16 parts of Astragalus membranaceus, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0124] (2) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Lysimachia christinae to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain extract and raffinate; dry the extract to obtain the first extract; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0125] The obtained extract was extracted with 10 times its weight of water at 75°C for 2 hours, filtered, and dried to obtain the second extract.

[0126] (3) After pulverizing Panax notoginseng and Astragalus membranaceus to 60 mesh, add them to water, then add cellulase, pectinase and β-glucosidase, and enzymatically hydrolyze at 46℃ for 2.5 h, then inactivate the enzymes at 90℃ for 10 min, filter, and dry to obtain the third extract; wherein, the total mass of Panax notoginseng and Astragalus membranaceus and the mass ratio of cellulase, pectinase, β-glucosidase and water is 1:0.006:0.006:0.004:12.

[0127] (4) After crushing the platycodon root, add 10 times the weight of water, extract at 75°C for 4 hours, filter, and dry to obtain the fourth extract; (5) Mix the first extract, second extract, third extract and fourth extract obtained above evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0128] Comparative Example 12 The difference between Comparative Example 12 and Example 1 is that the preparation method is different, but everything else is the same.

[0129] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of red root grass, 16 parts of Apocynum venetum leaf, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0130] (2) After pulverizing Salvia miltiorrhiza, Panax notoginseng, Apocynum venetum leaf, Curcuma zedoaria, Lysimachia christinae, and Platycodon grandiflorus to 60 mesh, add them to the extraction vessel for supercritical extraction to obtain the extract and raffinate; dry the extract to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction; wherein, the supercritical extraction uses CO2 as the extractant, 80wt% ethanol solution as the entrainer, CO2 flow rate is 60mL / min, supercritical extraction temperature is 40℃, pressure is 30MPa, and time is 80min.

[0131] Comparative Example 13 The difference between Comparative Example 13 and Example 1 is that the preparation method is different, but everything else is the same.

[0132] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of red root grass, 16 parts of Apocynum venetum leaf, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0133] (2) After crushing the danshen, sanqi, red root grass, apocynum leaf, zezhu, and platycodon, add 10 times the weight of water (10 times the total weight of danshen, sanqi, red root grass, apocynum leaf, zezhu, and platycodon), extract at 75℃ for 4 hours, filter, and dry to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0134] Comparative Example 14 The difference between Comparative Example 14 and Example 1 is that the preparation method is different, but everything else is the same.

[0135] A method for preparing a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction includes the following steps: (1) Weigh the following raw materials according to the mass ratio: 28 parts of Salvia miltiorrhiza, 18 parts of Panax notoginseng, 22 parts of red root grass, 16 parts of Apocynum venetum leaf, 10 parts of Curcuma zedoaria, and 6 parts of Platycodon grandiflorus.

[0136] (2) After pulverizing Salvia miltiorrhiza, Panax notoginseng, Lysimachia christinae, Apocynum venetum leaf, Curcuma zedoaria and Platycodon grandiflorus, add 10 times the mass of 60wt% ethanol solution (10 times the total mass of Salvia miltiorrhiza, Panax notoginseng, Lysimachia christinae, Apocynum venetum leaf, Curcuma zedoaria and Platycodon grandiflorus), extract at 75℃ for 4h, filter, dry, and obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

[0137] Test case 1. Select healthy male SD rats weighing 280±10g, with a 12 / 12hr diurnal rhythm, free feeding, and housed in a standard feeding environment for 5 days before the experiment. No adverse reactions were observed during the adaptation process.

[0138] Rats were randomly divided into groups of 8 (17 experimental groups and 1 model group). Rats were anesthetized intraperitoneally with chloral hydrate (350 mg / kg) and fixed in a supine position on a 37°C constant-temperature operating table. A midline incision was made in the neck, and the bilateral common carotid arteries were separated and clamped with miniature arterial clamps to induce cerebral ischemia. After 30 minutes, the arterial clamps were released to restore blood flow, the incision was sutured, and reperfusion was performed for 24 hours. The room temperature was maintained at 25°C, and the animal's body temperature was maintained at 37°C. Rats had free access to food and water. Each group of rats received the drug intervention via gavage for 7 days. The dosage of the traditional Chinese medicine composition was 150 mg / kg / day, administered twice daily; the model group received the same volume of purified water as the solvent daily in the same manner. Six hours after the last administration, rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (0.3 mL / kg). Samples were taken for TCC staining to detect the cerebral infarction area and calculate the reduction rate of cerebral infarction area.

[0139] The rate of reduction in cerebral infarction area = 100 * (cerebral infarction area in the model group - cerebral infarction area in the experimental group) / cerebral infarction area in the model group.

[0140] 2. Select healthy male SD rats weighing 280±10g, with a 12 / 12hr diurnal rhythm and free feeding. Before the experiment, they were housed in a standard feeding environment for 5 days. No adverse reactions were observed during the adaptation process.

[0141] Rats were randomly divided into groups of 8 (17 experimental groups and 1 model group). Rats were anesthetized, and one hour after the last administration, the fur on the left chest was clipped, and the area was disinfected with iodine and alcohol. The chest wall muscles and two ribs were cut 1 cm along the left sternal border to quickly open the thoracic cavity and expose the heart. The left coronary artery was ligated between the conus aorta and the left atrial appendage. The heart was immediately returned to its original position, air was expelled from the thoracic cavity, and the cavity was closed with hemostatic forceps to create an acute myocardial ischemia model in rats. Each group of rats received the drug via gavage for 7 days. The dosage of the traditional Chinese medicine composition was 150 mg / kg / day, administered twice daily. The model group received the same volume of purified water as the solvent daily in the same manner. Six hours after the last administration, the heart was removed, stained with TCC, and the infarct area was measured to calculate the reduction rate of infarct area.

[0142] The rate of reduction in myocardial infarction area = 100 (Infarct area in the model group - infarct area in the experimental group) / Infarct area in the model group.

[0143] Table 1

[0144] As can be seen from Table 1, the traditional Chinese medicine composition described in this application can effectively reduce the area of ​​cerebral infarction and myocardial infarction, and has excellent therapeutic effects on cerebral infarction and myocardial infarction.

[0145] Comparing Example 1 with Comparative Examples 1-2, it can be seen that the dosage of Salvia miltiorrhiza, Panax notoginseng, Lysimachia christinae, Apocynum venetum leaf, Curcuma zedoaria, and Platycodon grandiflorus has a significant effect on reducing the area of ​​cerebral infarction and myocardial infarction. This application can significantly reduce the area of ​​cerebral infarction and myocardial infarction by controlling the dosage of each raw material to: 25-30 parts Salvia miltiorrhiza, 15-22 parts Panax notoginseng, 17-25 parts Lysimachia christinae, 15-18 parts Apocynum venetum leaf, 8-12 parts Curcuma zedoaria, and 5-8 parts Platycodon grandiflorus.

[0146] Comparing Example 1 with Comparative Examples 3-11, it can be seen that the Salvia miltiorrhiza, Panax notoginseng, Lysimachia christinae, Apocynum venetum leaf, Curcuma zedoaria, and Platycodon grandiflorus described in this application have a significant synergistic effect in reducing the area of ​​cerebral infarction and myocardial infarction, and in treating cerebral infarction and myocardial infarction. Under the synergistic effect of Salvia miltiorrhiza, Panax notoginseng, Lysimachia christinae, Apocynum venetum leaf, Curcuma zedoaria, and Platycodon grandiflorus, the area of ​​cerebral infarction and myocardial infarction is effectively reduced, and the treatment effect of cerebral infarction and myocardial infarction is improved.

[0147] Comparing Example 1 with Comparative Examples 12-14, it can be seen that the preparation method of the traditional Chinese medicine composition has a significant impact on the effects of cerebral infarction and myocardial infarction. The specific preparation method of this application can significantly improve the treatment effect of cerebral infarction and myocardial infarction.

[0148] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction, characterized in that, The preparation materials include the following parts by weight: 25-30 parts of Salvia miltiorrhiza, 15-22 parts of Panax notoginseng, 17-25 parts of Lysimachia christinae, 15-18 parts of Apocynum venetum leaf, 8-12 parts of Curcuma zedoaria, and 5-8 parts of Platycodon grandiflorus.

2. The traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction according to claim 1, characterized in that, The raw materials include the following parts by weight: 28 parts Salvia miltiorrhiza, 18 parts Panax notoginseng, 22 parts Lysimachia christinae, 16 parts Apocynum venetum leaf, 10 parts Curcuma zedoaria, and 6 parts Platycodon grandiflorus.

3. The method for preparing the traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction as described in any one of claims 1 to 2, characterized in that, Includes the following steps: (1) After pulverizing Salvia miltiorrhiza, Curcuma zedoaria and Rhododendron simsii, supercritical extraction was performed to obtain extract and raffinate; the extract was dried to obtain the first extract; The residue was extracted with water, filtered, and dried to obtain the second extract. (2) After crushing Panax notoginseng and Apocynum venetum leaves, add them to water, then add cellulase, pectinase and β-glucosidase, hydrolyze, inactivate enzymes, dry, and obtain the third extract; (3) After crushing the platycodon root, extract it with water, filter it, and dry it to obtain the fourth extract; (4) The first extract, second extract, third extract and fourth extract are mixed evenly to obtain a traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction.

4. The method for preparing the traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction according to claim 3, characterized in that, The supercritical extraction uses CO2 as the extractant and 70-85 wt% ethanol solution as the entrainer, with a CO2 flow rate of 50-80 mL / min.

5. The method for preparing the traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction according to claim 3, characterized in that, The supercritical extraction temperature is 35~42℃, the pressure is 28~32Mpa, and the time is 70~100min.

6. The method for preparing the traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction according to claim 3, characterized in that, The mass ratio of the extract to water is 1:(8~20); Step (1) Water extraction temperature is 65~80℃, time is 1~4h.

7. The method for preparing the traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction according to claim 3, characterized in that, The total mass of Panax notoginseng and Apocynum venetum leaves, and the mass ratio of cellulase, pectinase, β-glucosidase, and water are 1:(0.005~0.008):(0.004~0.008):(0.003~0.005):(8~20).

8. The method for preparing the traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction according to claim 3, characterized in that, The enzymatic hydrolysis is performed at a temperature of 44-50℃ for 1-4 hours.

9. The method for preparing the traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction according to claim 3, characterized in that, The enzyme inactivation temperature is 85~95℃, and the time is 5~20min.

10. The method for preparing the traditional Chinese medicine composition for preventing and treating myocardial infarction and / or cerebral infarction according to claim 3, characterized in that, In step (3), the mass ratio of Platycodon grandiflorus to water is 1:(5~20), the water extraction temperature is 60~90℃, and the time is 1~6h.

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