A sodium hyaluronate mask patch material and a preparation method thereof

Abstract

The application belongs to the technical field of biological medicine, and specifically discloses a sodium hyaluronate mask patch material and a preparation method thereof. The sodium hyaluronate mask patch material comprises the following components in parts by mass: 0.1-0.3 parts of carbomer, 0.3-0.8 parts of p-hydroxyacetophenone, 0.1-1 parts of sodium hyaluronate, 0.3-0.8 parts of 1,2-hexanediol, 0.02-0.1 parts of sodium hydroxide, and 50-80 parts of water. The sodium hyaluronate comprises small-molecule sodium hyaluronate, large-molecule sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed oligomeric sodium hyaluronate. The sodium hyaluronate mask patch material has excellent repair effect.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, specifically to a sodium hyaluronate facial mask material and its preparation method. Background Technology

[0002] Wound dressings are important medical materials used to cover and protect skin injuries (such as those resulting from cosmetic procedures, minimally invasive surgery, abrasions, cuts, surgical incisions, chronic ulcers, and burn donor sites). An ideal wound dressing should possess the following characteristics: maintain a moist wound environment, absorb an appropriate amount of exudate, prevent contamination by external microorganisms, not adhere to the wound surface to reduce secondary damage during dressing changes, promote granulation tissue growth and epithelialization, and have good biocompatibility and be non-irritating.

[0003] Currently, commonly used wound dressings in clinical practice include traditional gauze / cotton pad dressings, hydrocolloid dressings, foam dressings, alginate dressings, hydrogel dressings, and medical functional dressings containing active ingredients. Traditional gauze dressings are dry and prone to sticking, causing further damage and pain to the wound during changes, and they cannot provide a moist environment conducive to healing. While hydrocolloid and foam dressings improve moisturizing and non-stickiness, most do not possess active biological activities such as promoting cell proliferation, anti-inflammation, and anti-oxidation. To enhance the repair function of dressings, loading dressings with active substances such as hyaluronic acid and collagen has become a research hotspot in recent years.

[0004] Sodium hyaluronate (SH) is an important component of the human extracellular matrix, possessing excellent moisturizing properties, biocompatibility, and the ability to promote wound healing, inhibit inflammatory responses, and reduce scar formation. Medical sodium hyaluronate wound dressings are already used clinically, with product forms including gel dressings, sprays, and patches. In existing products, sodium hyaluronate is usually added in a single molecular weight form, but different molecular weights of sodium hyaluronate have different effects on wound healing. A single molecular weight cannot simultaneously exert the dual effects of surface barrier protection and deep tissue repair.

[0005] Furthermore, existing medical dressings often contain chemical preservatives such as methylparaben and phenoxyethanol for corrosion prevention. Studies have shown that some preservatives may irritate wound tissue or cause cytotoxicity, delaying the healing process, especially for chronic ulcers or deep burns. Therefore, developing a wound dressing that is free of irritating preservatives and possesses moisturizing, repairing, and non-adhesive properties is of significant clinical importance. Summary of the Invention

[0006] This invention provides a sodium hyaluronate facial mask material and its preparation method. The sodium hyaluronate facial mask material described in this application has excellent repair effects.

[0007] The present invention solves its technical problem by adopting the following technical solution: A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.1-0.3 parts carbomer, 0.3-0.8 parts p-hydroxyacetophenone, 0.1-1 part sodium hyaluronate, 0.3-0.8 parts 1,2-hexanediol, 0.02-0.1 parts sodium hydroxide, and 50-80 parts water; The sodium hyaluronate includes small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate.

[0008] As an embodiment of this application, the components include the following parts by weight: 0.12-0.25 parts carbomer, 0.4-0.6 parts p-hydroxyacetophenone, 0.2-0.8 parts sodium hyaluronate, 0.3-0.6 parts 1,2-hexanediol, 0.04-0.08 parts sodium hydroxide, and 60-80 parts water.

[0009] As an embodiment of this application, the mass ratio of the small molecule sodium hyaluronate, the large molecule sodium hyaluronate, the acetylated sodium hyaluronate and the hydrolyzed oligomeric sodium hyaluronate is (26~35):(12~20):(18~29):(25~35).

[0010] As an embodiment of this application, the mass ratio of the small molecule sodium hyaluronate, the large molecule sodium hyaluronate, the acetylated sodium hyaluronate and the hydrolyzed oligomeric sodium hyaluronate is (30~32):(15~18):(23~24):(28~30).

[0011] As an embodiment of this application, the molecular weight of the small molecule sodium hyaluronate is 20~40 kDa.

[0012] As an embodiment of this application, the molecular weight of the hydrolyzed oligomeric sodium hyaluronate is <10 kDa.

[0013] As an embodiment of this application, the hydrolyzed oligomeric sodium hyaluronate has a molecular weight of 5-8 kDa.

[0014] As an embodiment of this application, the molecular weight of the macromolecular sodium hyaluronate is 300~400 kDa.

[0015] As an embodiment of this application, the acetylated sodium hyaluronate has a molecular weight of 8-15 kDa and a degree of acetyl substitution of 2-2.8.

[0016] This application also provides a method for preparing a sodium hyaluronate facial mask material, comprising the following steps: Mix water and carbomer until they swell, add sodium hyaluronate and mix well, then add 1,2-hexanediol and p-hydroxyacetophenone and mix well. Finally, add sodium hydroxide and mix well. Let stand and degas under vacuum to obtain sodium hyaluronate facial mask material.

[0017] The beneficial effects of this invention are as follows: The sodium hyaluronate facial mask material described in this application uses carbomer as a thickener, which can provide suitable viscosity and spreadability. The addition of sodium hydroxide neutralizes the carboxyl groups of carbomer, causing it to form a pseudoplastic gel. In the specific pseudoplastic gel system of this application, by adding small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed oligomeric sodium hyaluronate, a stable hydration film can be formed on the wound surface, maintaining a moist environment and preventing bacterial invasion. At the same time, it can be effectively adsorbed on the stratum corneum at the wound edge (without adhesion), prolonging the action time, and effectively penetrating into the deep layers of the wound surface, directly acting on fibroblasts and keratinocytes, promoting cell migration and proliferation, and effectively improving the repair effect. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0020] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0021] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0022] Unless otherwise specified, all reagents or instruments used in this invention are commercially available conventional products. Unless otherwise specified, the raw materials used in each comparative example and the parallel experiments of each embodiment are the same commercially available products.

[0023] This application provides a sodium hyaluronate facial mask material, comprising the following components in parts by weight: 0.1-0.3 parts carbomer, 0.3-0.8 parts p-hydroxyacetophenone, 0.1-1 part sodium hyaluronate, 0.3-0.8 parts 1,2-hexanediol, 0.02-0.1 parts sodium hydroxide, and 50-80 parts water; The sodium hyaluronate includes small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate.

[0024] The sodium hyaluronate facial mask material described in this application uses carbomer as a thickener, which provides suitable viscosity and spreadability. Sodium hydroxide is added to neutralize the carboxyl groups of carbomer, forming a pseudoplastic gel. In the specific pseudoplastic gel system of this application, by adding small-molecule sodium hyaluronate, large-molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate, a stable hydration film can be formed on the wound surface, maintaining a moist environment and preventing bacterial invasion. Simultaneously, it can effectively adsorb onto the stratum corneum at the wound edge (without adhesion), prolonging the action time and effectively penetrating deep into the wound surface, directly acting on fibroblasts and keratinocytes, promoting cell migration and proliferation, and effectively improving the repair effect.

[0025] The small-molecule sodium hyaluronate activates the TLR-2 and TLR-4 signaling pathways, inducing macrophages to secrete pro-inflammatory cytokines to initiate a repair response, while also strongly stimulating endothelial cell proliferation and fibroblast migration. The large-molecule sodium hyaluronate binds to the CD44 receptor, promoting keratinocyte migration and early granulation tissue formation, while also forming a hydration barrier to provide moisturizing and lubrication. The acetylated sodium hyaluronate adheres tightly to the wound edge, prolonging the action time of the active ingredients. Furthermore, the large-molecule sodium hyaluronate and the acetylated sodium hyaluronate can form a stable hydration film, maintaining a moist environment and preventing bacterial invasion. The hydrolyzed oligomeric sodium hyaluronate component penetrates deep into the wound surface, directly acting on fibroblasts and keratinocytes to promote cell migration and proliferation. This application effectively improves the repair effect through the synergistic effect of small-molecule sodium hyaluronate, large-molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate.

[0026] In this application, p-hydroxyacetophenone and hexanediol are used as a preservative system. P-hydroxyacetophenone has antifungal and partial antibacterial activity, while hexanediol is a polyol that acts as a moisturizer and preservative enhancer. Together, they provide a broad-spectrum preservative effect. Compared with traditional parabens and phenoxyethanol, this system significantly reduces cytotoxicity and has no skin sensitization, making it particularly suitable for sensitive areas such as wounds and mucous membranes.

[0027] This application uses carbomer and sodium hydroxide as the gelling system, with carbomer as the gel matrix. After neutralization with sodium hydroxide, it forms a highly transparent, low-viscosity (easy to squeeze out and spread) gel with pseudoplasticity. When applied to the wound, this gel forms a soft, moist film that does not mechanically adhere to the wound after drying. It can be easily rinsed away with saline solution during dressing changes, avoiding secondary damage and pain.

[0028] The amount of carbomer used is 0.1 to 0.3 parts, for example, it can be 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, or any two of these values.

[0029] The amount of p-hydroxyacetophenone used is 0.3 to 0.8 parts, for example, it can be 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts or any two of these values.

[0030] The amount of sodium hyaluronate used is 0.1 to 1 part, for example, it can be 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part or any two of these values.

[0031] The amount of 1,2-hexanediol used is 0.3 to 0.8 parts, for example, it can be 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, or any two of these values.

[0032] The amount of sodium hydroxide used is 0.02 to 0.1 parts, for example, it can be 0.02 parts, 0.03 parts, 0.04 parts, 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts, 0.09 parts, 0.1 parts, or any two of these values.

[0033] The amount of water used is 50 to 80 parts, for example, it can be 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts or any two of these values.

[0034] In some embodiments, the components include the following parts by weight: 0.12-0.25 parts carbomer, 0.4-0.6 parts p-hydroxyacetophenone, 0.2-0.8 parts sodium hyaluronate, 0.3-0.6 parts 1,2-hexanediol, 0.04-0.08 parts sodium hydroxide, and 60-80 parts water.

[0035] In some embodiments, the mass ratio of the small molecule sodium hyaluronate, the large molecule sodium hyaluronate, the acetylated sodium hyaluronate and the hydrolyzed oligomeric sodium hyaluronate is (26~35):(12~20):(18~29):(25~35).

[0036] In some embodiments, the mass ratio of the small molecule sodium hyaluronate, the large molecule sodium hyaluronate, the acetylated sodium hyaluronate and the hydrolyzed oligomeric sodium hyaluronate is (30~32):(15~18):(23~24):(28~30).

[0037] In some embodiments, the molecular weight of the small molecule sodium hyaluronate is 20-40 kDa.

[0038] In some embodiments, the molecular weight of the hydrolyzed oligomeric sodium hyaluronate is <10 kDa.

[0039] In some embodiments, the hydrolyzed oligomeric sodium hyaluronate has a molecular weight of 5-8 kDa.

[0040] In some embodiments, the molecular weight of the macromolecular sodium hyaluronate is 300-400 kDa.

[0041] In some embodiments, the acetylated sodium hyaluronate has a molecular weight of 8-15 kDa and a degree of acetyl substitution of 2-2.8.

[0042] It should be noted that this application does not limit the source of large molecular weight sodium hyaluronate, small molecular weight sodium hyaluronate, or hydrolyzed oligomeric sodium hyaluronate, as long as their molecular weight meets the requirements of this application. For example, this application can be obtained by purchasing commercially available products from companies such as Bloomage Freda, Solarbio Science & Technology, and Zhongshan Biotechnology.

[0043] It should be noted that the source of acetylated sodium hyaluronate is not limited in this application, as long as its molecular weight and degree of acetyl substitution meet the requirements of this application. For example, this application can be obtained by purchasing commercially available products from companies such as Bloomage Freda and Zhongshan Biotechnology.

[0044] This application also provides a method for preparing a sodium hyaluronate facial mask material, comprising the following steps: Mix water and carbomer until they swell, add sodium hyaluronate and mix well, then add 1,2-hexanediol and p-hydroxyacetophenone and mix well. Finally, add sodium hydroxide and mix well. Let stand and degas under vacuum to obtain sodium hyaluronate facial mask material.

[0045] The present application is further illustrated below with specific embodiments:

[0046] Example 1 A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0047] The sodium hyaluronate comprises small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 30:18:24:28.

[0048] Among them, the molecular weight of small molecule sodium hyaluronate is 40 kDa.

[0049] Among them, the molecular weight of hydrolyzed oligomeric sodium hyaluronate is 7 kDa.

[0050] Among them, the molecular weight of the macromolecular sodium hyaluronate is 400 kDa.

[0051] Among them, the molecular weight of acetylated sodium hyaluronate is 10 kDa and the degree of acetyl substitution is 2.6.

[0052] The preparation method of sodium hyaluronate facial mask material includes the following steps: Add water to the mixing tank, adjust the temperature to 25℃, turn on the stirring vessel, and stir at 1000 rpm. Add carbomer 980 and stir at 1000 rpm for 20 minutes. Stop stirring and let it stand to swell for 1 hour. Add sodium hyaluronate and stir at 1000 rpm for 15 minutes. Then add 1,2-hexanediol and p-hydroxyacetophenone, adjust the temperature to 82℃, and stir at 30 rpm for 20 minutes at 82℃ to dissolve it into a clear liquid. Then add sodium hydroxide and stir evenly at 30 rpm. Let it stand for 30 minutes and degas under vacuum to obtain sodium hyaluronate facial mask material.

[0053] Example 2 Example 2 differs from Example 1 in that the mass ratio of small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate is different, while all other aspects are the same.

[0054] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0055] The sodium hyaluronate comprises small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 32:15:23:30.

[0056] Example 3 Example 3 differs from Example 1 in that the mass ratio of small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate is different, while all other aspects are the same.

[0057] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0058] The sodium hyaluronate comprises small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 35:12:18:35.

[0059] Example 4 Example 4 differs from Example 1 in that the mass ratio of small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate is different, while all other aspects are the same.

[0060] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0061] The sodium hyaluronate comprises small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 26:20:29:25.

[0062] Example 5 Example 5 differs from Example 1 in that the molecular weights of the small-molecule sodium hyaluronate and the large-molecule sodium hyaluronate are different, but everything else is the same.

[0063] Among them, the molecular weight of small molecule sodium hyaluronate is 20 kDa.

[0064] Among them, the molecular weight of the macromolecular sodium hyaluronate is 300 kDa.

[0065] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the mass ratios of small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate are different, while all other aspects are the same.

[0066] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0067] The sodium hyaluronate comprises small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 15:30:37:18.

[0068] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the mass ratios of small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate are different, while all other aspects are the same.

[0069] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0070] The sodium hyaluronate comprises small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 45:5:5:45.

[0071] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain acetylated sodium hyaluronate, but all other aspects are the same.

[0072] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0073] The sodium hyaluronate comprises small molecule sodium hyaluronate, large molecule sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 30:18:24.

[0074] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain hydrolyzed oligomeric sodium hyaluronate, but all other aspects are the same.

[0075] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0076] The sodium hyaluronate comprises small molecule sodium hyaluronate, large molecule sodium hyaluronate, and acetylated sodium hyaluronate in a mass ratio of 30:18:24:28.

[0077] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain small molecule sodium hyaluronate, but all other aspects are the same.

[0078] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0079] The sodium hyaluronate comprises macromolecular sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 18:24:28.

[0080] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not contain large molecular weight sodium hyaluronate, but all other aspects are the same.

[0081] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0082] The sodium hyaluronate comprises small molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 30:24:28.

[0083] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 does not contain acetylated sodium hyaluronate and hydrolyzed oligomeric sodium hyaluronate, but all other aspects are the same.

[0084] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0085] The sodium hyaluronate comprises small-molecule sodium hyaluronate and large-molecule sodium hyaluronate in a mass ratio of 30:18.

[0086] Comparative Example 8 The difference between Comparative Example 8 and Example 1 is that Comparative Example 8 does not contain large molecule sodium hyaluronate or small molecule sodium hyaluronate, but all other aspects are the same.

[0087] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0088] The sodium hyaluronate comprises acetylated sodium hyaluronate and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 24:28.

[0089] Comparative Example 9 The difference between Comparative Example 9 and Example 1 is that the sodium hyaluronate in Comparative Example 9 is different from that in Example 1, but everything else is the same.

[0090] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0091] The sodium hyaluronate comprises ultra-high molecular weight sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 48:24:28.

[0092] Among them, the molecular weight of ultra-high molecular weight sodium hyaluronate is 1500 kDa.

[0093] Comparative Example 10 The difference between Comparative Example 10 and Example 1 is that the sodium hyaluronate in Comparative Example 10 is different from that in Example 1, but everything else is the same.

[0094] A sodium hyaluronate facial mask material comprises the following components in parts by weight: 0.18 parts carbomer 980, 0.5 parts p-hydroxyacetophenone, 0.4 parts sodium hyaluronate, 0.4 parts 1,2-hexanediol, 0.06 parts sodium hydroxide, and 78.46 parts water.

[0095] The sodium hyaluronate comprises medium molecular weight sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate in a mass ratio of 48:24:28.

[0096] Among them, the molecular weight of medium molecular weight sodium hyaluronate is 100 kDa.

[0097] Test case Cytotoxicity: The test shall be conducted according to the MTT assay in GB / T 16886.5-2017 Biological Evaluation of Medical Devices Part 5: In Vitro Cytotoxicity Test Methods.

[0098] Table 1

[0099] As can be seen from Table 1, the sodium hyaluronate facial mask material described in this application has good safety and meets the requirement of ≥70%.

[0100] 2. Repair Effect: Ninety SD rats with similar growth status (e.g., weight, fur condition) were randomly divided into 15 groups (n=6 per group). After anesthetizing the rats, the hair on their backs was removed using a depilatory agent (10% sodium sulfide). Paraffin droplets were then applied to the backs of the rats to create a 3cm × 3cm (9cm) area. 2 A full-thickness skin injury model (i.e., the initial wound area).

[0101] The dressings used in the examples and comparative examples were applied to the wounds, with a thickness controlled at 2.5 mm (±0.1 mm). Application was performed once daily for 14 consecutive days. The wound healing area on the 14th day was recorded, and the wound healing rate was calculated as follows: Wound healing rate = (Wound healing area on the 14th day / 9). 100%, the results are shown in Table 2.

[0102] Table 2

[0103] As can be seen from Table 2, the sodium hyaluronate facial mask material described in this application has excellent repair effects.

[0104] Comparing Examples 1-2 with Examples 3-4, it can be seen that by controlling the mass ratio of small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed oligomeric sodium hyaluronate to (30-32): (15-18): (23-24): (28-30), this application can further improve the repair effect.

[0105] Comparing Examples 1-4 with Comparative Examples 1-2, it can be seen that by controlling the mass ratio of small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed oligomeric sodium hyaluronate to (26-35): (12-20): (18-29): (25-35), this application can significantly improve the repair effect.

[0106] Comparing Example 1 with Comparative Examples 3-10, it can be seen that the small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate and hydrolyzed oligomeric sodium hyaluronate described in this application have a significant synergistic effect in improving the repair effect.

[0107] 3. Cell Adhesion Rate: The sodium hyaluronate facial mask materials from Examples 1 and 2 were spread evenly across the bottom of the culture plates. Human fibroblasts were selected as experimental cells, and experimental and control groups were set up. The experimental group had 2 × 10⁶ cells seeded onto the surface of each well. 4Add 2 mL of complete culture medium (DMEM basal medium + 10% fetal bovine serum) to each well. Incubate the culture plate in a 37°C, 5% CO2 incubator for 4 hours. After incubation, carefully aspirate the culture medium from the wells and gently rinse each well three times with sterile PBS to thoroughly remove any unadhered or floating free cells, retaining only the live cells firmly adhered to the wound dressing material and the bottom of the plate. After rinsing, add 20 μL of 0.5% MTT-PBS working solution to each well and continue incubating at 37°C in the dark for 4 hours to promote the reaction between mitochondrial dehydrogenase and MTT in the live cells, generating blue-purple crystals. After incubation, aspirate the supernatant from the wells and add 200 μL of dimethyl sulfoxide (DMSO) to each well, shaking at low speed for 10 minutes at room temperature to completely dissolve the crystals. Once the solution is homogeneous and clear, measure the absorbance (OD) of each well at 570 nm using a microplate reader.

[0108] Each group had 3 parallel replicates, and the experiment was repeated 3 times. The test results are shown in Table 2. The cell adhesion rate was calculated using the following formula: Cell adhesion rate (%) = (Corrected OD value of experimental group / Corrected OD value of blank control group) × 100%. Corrected OD value of experimental group = Measured OD value of experimental group - Mean OD value of blank well.

[0109] Among them, the cell adhesion rates of Examples 1 and 2 were 16.2% and 16.7%, respectively. It can be seen that the sodium hyaluronate mask dressing material described in this application has a certain anti-adhesion effect, which can avoid adhesion to the wound and avoid secondary damage and pain during replacement.

[0110] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A sodium hyaluronate facial mask material, characterized in that, The components include the following parts by weight: 0.1-0.3 parts carbomer, 0.3-0.8 parts p-hydroxyacetophenone, 0.1-1 parts sodium hyaluronate, 0.3-0.8 parts 1,2-hexanediol, 0.02-0.1 parts sodium hydroxide, and 50-80 parts water; The sodium hyaluronate includes small molecule sodium hyaluronate, large molecule sodium hyaluronate, acetylated sodium hyaluronate, and hydrolyzed oligomeric sodium hyaluronate.

2. The sodium hyaluronate facial mask material according to claim 1, characterized in that, The components include the following parts by weight: 0.12-0.25 parts carbomer, 0.4-0.6 parts p-hydroxyacetophenone, 0.2-0.8 parts sodium hyaluronate, 0.3-0.6 parts 1,2-hexanediol, 0.04-0.08 parts sodium hydroxide, and 60-80 parts water.

3. The sodium hyaluronate facial mask material according to claim 1, characterized in that, The mass ratio of the small molecule sodium hyaluronate, the large molecule sodium hyaluronate, the acetylated sodium hyaluronate and the hydrolyzed oligomeric sodium hyaluronate is (26~35):(12~20):(18~29):(25~35).

4. The sodium hyaluronate facial mask material according to claim 1, characterized in that, The mass ratio of the small molecule sodium hyaluronate, the large molecule sodium hyaluronate, the acetylated sodium hyaluronate and the hydrolyzed oligomeric sodium hyaluronate is (30~32):(15~18):(23~24):(28~30).

5. The sodium hyaluronate facial mask material according to claim 1, characterized in that, The molecular weight of the small molecule sodium hyaluronate is 20~40 kDa.

6. The sodium hyaluronate facial mask material according to claim 1, characterized in that, The molecular weight of the hydrolyzed oligomeric sodium hyaluronate is <10 kDa.

7. The sodium hyaluronate facial mask material according to claim 1, characterized in that, The molecular weight of the hydrolyzed oligomeric sodium hyaluronate is 5~8 kDa.

8. The sodium hyaluronate facial mask material according to claim 1, characterized in that, The molecular weight of the high molecular weight sodium hyaluronate is 300~400 kDa.

9. The sodium hyaluronate facial mask material according to claim 1, characterized in that, The acetylated sodium hyaluronate has a molecular weight of 8-15 kDa and a degree of acetyl substitution of 2-2.

8.

10. A method for preparing the sodium hyaluronate facial mask material according to any one of claims 1 to 9, characterized in that, Includes the following steps: Mix water and carbomer until they swell, add sodium hyaluronate and mix well, then add 1,2-hexanediol and p-hydroxyacetophenone and mix well. Finally, add sodium hydroxide and mix well. Let stand and degas under vacuum to obtain sodium hyaluronate facial mask material.

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