A molecular marker assisted breeding device

Abstract

The application discloses a molecular marker assisted breeding device and relates to the technical field of molecular breeding assistance, which comprises a reagent storage box, a fixed slide rail is fixedly installed at the bottom of the inside of the reagent storage box, a bearing block is slidably connected to the outer surface of the fixed slide rail, a reagent tube is placed and installed in the inside of the bearing block, a baffle is fixedly installed on the front of the bearing block, handles are fixedly installed on the two sides of the front of the baffle, the bottom of the baffle is slidably connected to the outer surface of the fixed slide rail, and a staggered jacking mechanism is arranged. When an operator needs to add multiple reagents in sequence, the operator only needs to rotate and twist the blocks in sequence, so that the rotating rods drive the cams one and two to jack up the lugs one and two in sequence, and then the operator can open the cover plates one and two in the preset sequence one by one, and the corresponding reagent tubes are exposed, so that the operator can avoid the human errors such as skipping, missing or repeatedly adding reagents when the operator freely takes and uses the reagents according to memory.

Description

Technical Field

[0001] This invention relates to the field of molecular breeding assistance technology, specifically to a molecular marker-assisted breeding device. Background Technology

[0002] Molecular marker-assisted breeding is a modern breeding technique that uses DNA molecular markers to assist in the selection of superior traits. By detecting the presence of markers, it indirectly determines whether the target gene has been inherited. This allows for precise screening of traits such as disease resistance, quality, and growth period in breeding materials at the seedling stage or even the seed stage. It does not rely on phenotypic observation after plant maturity, effectively eliminates environmental interference, shortens the breeding cycle, improves selection accuracy, and supports the simultaneous aggregation of multiple superior genes. In the process, researchers need to extract DNA from the breeding sample, aliquot it into test tubes, and store it in a kit for subsequent analysis such as PCR amplification, electrophoresis, or fluorescence detection, achieving efficient reverse selection from genotype to phenotype.

[0003] In existing technologies, when sample tubes are stored in the kit, after DNA extraction, operators aliquot the DNA solution of each breeding sample into 0.2ml or 1.5ml microcentrifuge tubes according to a pre-set procedure and insert the tubes into the corresponding wells of the kit. To ensure long-term sample stability and enzyme activity preservation, the entire kit is transferred to a -20℃ freezer or a low-temperature transport box containing ice packs. A one-dimensional or two-dimensional code label containing the sample number, date, and plate number is affixed to the lid or side of the box. During PCR amplification and other detection steps, operators directly remove the entire plate of test tubes from the kit to thaw, or manually move them. However, when using the kit again, a single reaction requires the addition of multiple reagents in sequence. The positions of the reagent tubes in the box are fixed, and they cannot be retrieved in a fixed order. Therefore, the lack of a sequence locking mechanism makes it difficult to standardize the operation process. Different personnel may add samples in different orders, resulting in poor reproducibility of the results of the same batch of experiments. This not only wastes breeding samples and expensive molecular reagents, but also leads to the incorrect elimination of superior individual plants due to incorrect genotype interpretation, reducing the efficiency and success rate of the entire molecular marker-assisted breeding process.

[0004] Although sampling can be performed in sequence, the sampling direction cannot be locked during sampling, resulting in a rotation phenomenon. To ensure the correctness of the sequence, if the sampling is incorrect, it is impossible to trace back to the specific step where the regression occurred through mechanical records. Breeders can only discard the entire plate of samples and re-experiment, which not only wastes breeding materials but also causes them to miss the optimal sowing time due to repeated re-examinations, which is not conducive to more efficient operation and use in practice. Summary of the Invention

[0005] The purpose of this invention is to provide a molecular marker-assisted breeding device to solve the problem of locking the operation while performing the operation in sequence.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a molecular marker-assisted breeding device, comprising a reagent storage box, a fixed slide rail fixedly installed at the bottom of the reagent storage box, a receiving block slidably connected to the outer surface of the fixed slide rail, a reagent tube placed inside the receiving block, a baffle fixedly installed on the front of the receiving block, handles fixedly installed on both sides of the front of the baffle, the bottom of the baffle slidably connected to the outer surface of the fixed slide rail, staggered lifting mechanisms installed on both sides of the reagent storage box, and a locking mechanism that converts rotational force into locking force installed on the output shaft of the staggered lifting mechanism.

[0007] Preferably, the staggered lifting mechanism includes a limiting block, a rotating rod, a screwing block, a cam, a protrusion, and a cover plate. The left side of the limiting block is fixedly installed with the right side of the reagent storage box. The outer surface of the rotating rod is rotatably installed with the inside of the limiting block. The back of the screwing block is fixedly installed with one end of the rotating rod. The inside of the cam is fixedly installed with the outer surface of the rotating rod. The lower surface of the cover plate is in contact with the upper surface of the reagent storage box. The upper surface of the protrusion is fixedly installed with the lower surface of the cover plate. The lower surface of the protrusion is in contact with the outer surface of the cam.

[0008] Preferably, the locking mechanism includes a T-shaped plate, a rotating shaft, a pawl, and a ratchet. The left side of the T-shaped plate is fixedly installed with the right side of the reagent storage box. The outer surface of the rotating shaft is rotatably installed with the inner wall of the T-shaped plate. The inside of the pawl is fixedly installed with the outer surface of the rotating shaft. The ratchet is fitted onto the surface of the pawl.

[0009] Preferably, the upper surface of the reagent storage box is provided with a rectangular groove, a connecting spring is fixedly installed at the bottom of the rectangular groove, and a telescopic rod is fixedly installed at the bottom of the rectangular groove.

[0010] Preferably, one end of the rectangular groove and the connecting spring are both fixedly installed on the lower surface of the cover plate.

[0011] Preferably, a second cam is fixedly mounted on the outer surface of the rotating rod, and the initial phase position of the second cam is different from that of the first cam.

[0012] Preferably, a second protrusion is fitted onto the outer surface of the second cam, a second cover plate is fixedly fitted onto the upper surface of the second protrusion, and the lower surface of the second cover plate is fitted onto the upper surface of the reagent storage box.

[0013] Preferably, the inside of the ratchet is fixedly installed on the outer surface of one end of the rotating rod to prevent the rotating rod from rotating back during rotation.

[0014] Preferably, a fixed shaft is rotatably mounted on the inner wall of the front of the reagent storage box, and a rotating plate is fixedly mounted on the outer surface of the fixed shaft.

[0015] Preferably, the rotating plate is threaded with a hand-tightening screw, and the outer surface of the hand-tightening screw is rotatably mounted to the inner wall of the baffle.

[0016] Compared with the prior art, the beneficial effects of the present invention are: This invention, through the setting of a staggered lifting mechanism, allows operators to add multiple reagents sequentially by rotating the screw block. This causes the rotating rod to drive cam one and cam two to lift protrusion one and protrusion two in sequence, thereby opening cover one and cover two one by one in a preset order to expose the corresponding reagent tubes. This avoids human errors such as skipping steps, missing additions, or repeated additions that may occur when operators rely on memory to freely select reagents. It ensures that the sample addition process can be completely unified among different personnel, achieving standardization of the operation process and improving the reproducibility of experimental results in the same batch. This not only avoids the unnecessary waste of breeding samples and molecular reagents but also improves the efficiency and success rate of molecular marker-assisted breeding.

[0017] This invention employs a locking mechanism, with a ratchet fixedly mounted on the outer surface of the rotating rod. A pawl, engaging with the ratchet surface, is installed via a rotating shaft on a T-plate. This ensures that the rotating rod can only rotate in one direction when picking up reagents, preventing reverse rotation and avoiding unintentional backtracking due to fatigue or distraction. Even if sample addition errors occur, the mechanical structure records the irreversible process of the operation, allowing breeders to accurately trace the error step without discarding the entire sample and re-testing. This avoids missing the optimal sowing time due to repeated re-testing and ensures that superior plants are not mistakenly eliminated, facilitating efficient and reliable operation of molecular marker-assisted breeding in actual production. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 For the present invention Figure 1 Top view of the structure; Figure 3 For the present invention Figure 1 A schematic diagram of the structure viewed from below; Figure 4 For the present invention Figure 1 Internal structure diagram; Figure 5 For the present invention Figure 4 Enlarged schematic diagram of the structure at point A; Figure 6 For the present invention Figure 4 A schematic diagram of the side view structure; Figure 7 For the present invention Figure 6 Enlarged schematic diagram of the structure at point B; Figure 8 For the present invention Figure 6 A magnified schematic diagram of the structure at point C.

[0019] In the diagram: 1. Reagent storage box; 2. Fixed slide rail; 3. Baffle; 4. Handle; 5. Receiving block; 6. Reagent tube; 7. Fixed shaft; 8. Rotating plate; 9. Hand screw; 10. Staggered lifting mechanism; 101. Limiting block; 102. Rotating rod; 103. Tightening block; 104. Cam 1; 105. Protrusion 1; 106. Cover plate 1; 107. Rectangular groove; 108. Connecting spring; 109. Telescopic rod; 1010. Cam 2; 1011. Protrusion 2; 1012. Cover plate 2; 11. Locking mechanism; 111. T-plate; 112. Rotating shaft; 113. Pawl; 114. Ratchet. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Please see Figure 2 , Figure 4 , Figure 5 , Figure 6 and Figure 7As shown, the present invention provides a technical solution: a molecular marker-assisted breeding device, including a reagent storage box 1, a fixed slide rail 2 fixedly installed at the bottom of the reagent storage box 1, a receiving block 5 slidably connected to the outer surface of the fixed slide rail 2, a reagent tube 6 placed inside the receiving block 5, a baffle 3 fixedly installed on the front of the receiving block 5, handles 4 fixedly installed on both sides of the front of the baffle 3, the bottom of the baffle 3 slidably connected to the outer surface of the fixed slide rail 2, staggered lifting mechanisms 10 installed on both sides of the reagent storage box 1, a locking mechanism 11 for converting rotational force into locking force installed on the output shaft of the staggered lifting mechanism 10, the staggered lifting mechanism 10 including a limit block 101, a rotating rod 102, a twisting block 103, a cam 104, a protrusion 105 and a cover plate 106, the left side of the limit block 101 fixedly installed with the right side of the reagent storage box 1, the outer surface of the rotating rod 102 rotatably installed with the inside of the limit block 101, and the back of the twisting block 103 rotatably installed with the rotating rod 102. One end is fixedly installed. The inside of cam 104 is fixedly installed with the outer surface of rotating rod 102. The lower surface of cover plate 106 is in contact with the upper surface of reagent storage box 1. The upper surface of protrusion 105 is fixedly installed with the lower surface of cover plate 106. The lower surface of protrusion 105 is in contact with the outer surface of cam 104. A rectangular groove 107 is formed on the upper surface of reagent storage box 1. A connecting spring 108 is fixedly installed at the bottom of the rectangular groove 107. The bottom of the rectangular groove 107 is fixedly installed. The telescopic rod 109 is installed. One end of the rectangular groove 107 and the connecting spring 108 are fixedly installed on the lower surface of the cover plate 106. The outer surface of the rotating rod 102 is fixedly installed with the cam 1010. The initial phase position of the cam 1010 is different from that of the cam 104. The outer surface of the cam 1010 is fitted with the protrusion 1011. The upper surface of the protrusion 1011 is fixedly installed with the cover plate 1012. The lower surface of the cover plate 1012 is fitted with the upper surface of the reagent storage box 1.

[0022] Specifically, by setting a fixed slide rail 2 at the bottom of the reagent storage box 1, the receiving block 5 can slide on the fixed slide rail 2, and the reagent tube 6 is placed inside the receiving block 5. With the design of the baffle 3 and handle 4, the operator can easily move the baffle 3 and the receiving block 5 along the fixed slide rail 2 by pulling the handle 4, thereby quickly taking out or placing the reagent tube 6, improving the efficiency of reagent retrieval and saving operation time. The staggered lifting mechanism 10 allows for more flexible and orderly opening and closing of cover plate 106 and cover plate 1012. Twisting the rotating block 103 drives the rotating rod 102 to rotate, which in turn causes cam 104 and cam 1010 to rotate. Since cam 104 and cam 1010 have different initial phase positions, during rotation, cam 104 engages with protrusion 105, and cam 1010 engages with protrusion 1011. This allows for separate control of cover plate 106 and cover plate 1012 to open and close at different times and in different sequences, meeting the opening requirements of reagent storage box 1 under different operating scenarios and avoiding the inconvenience of opening all covers at once. This also reduces the risk of reagent contamination from the external environment. A connecting spring 108 and a telescopic rod 109 are installed in the rectangular groove 107. When the first cam 104 and the second cam 1010 rotate to raise the first cover 106 and the second cover 1012, the first cover 106 and the second cover 1012 can be reliably reset under the elastic action of the connecting spring 108, ensuring that the reagent storage box 1 is in a good sealed state and protecting the reagent from external factors. At the same time, the telescopic rod 109 can provide a stable guide for the lifting and lowering of the cover, ensuring the smoothness of the cover movement.

[0023] according to Figure 1 , Figure 4 , Figure 6 and Figure 8 As shown, the locking mechanism 11 includes a T-shaped plate 111, a rotating shaft 112, a pawl 113, and a ratchet 114. The left side of the T-shaped plate 111 is fixedly installed with the right side of the reagent storage box 1. The outer surface of the rotating shaft 112 is rotatably installed with the inner wall of the T-shaped plate 111. The inside of the pawl 113 is fixedly installed with the outer surface of the rotating shaft 112. The ratchet 114 is fitted into the surface of the pawl 113. The inside of the ratchet 114 is fixedly installed with the outer surface of one end of the rotating rod 102 to prevent the rotating rod 102 from rotating back during rotation.

[0024] Specifically, the locking mechanism 11 employs a pawl 113 and a ratchet 114. When the rotating rod 102 rotates, the ratchet 114 rotates accordingly, and the pawl 113 slides between the teeth of the ratchet 114, allowing the rotating rod 102 to rotate normally in one direction. When a reverse force attempts to make the rotating rod 102 rotate back, the pawl 113 engages with the teeth of the ratchet 114, preventing it from rotating back. This avoids the rotating rod 102 from rotating back during operation, ensuring the stability and accuracy of the staggered lifting mechanism 10. It also ensures that the first cover plate 106 and the second cover plate 1012 can open and close according to predetermined requirements, preventing any unexpected situations caused by the rotation of the rotating rod 102.

[0025] according to Figure 1 , Figure 2 , Figure 3 and Figure 4As shown, a fixed shaft 7 is rotatably installed on the inner wall of the front of the reagent storage box 1, and a rotating plate 8 is fixedly installed on the outer surface of the fixed shaft 7. A hand screw 9 is installed on the internal thread of the rotating plate 8, and the outer surface of the hand screw 9 is rotatably installed with the inner wall of the baffle 3.

[0026] Specifically, a fixed shaft 7 is rotatably installed on the inner wall of the front of the reagent storage box 1, and a rotating plate 8 is fixed on the outer surface of the fixed shaft 7. A hand-tightening screw 9, threaded inside the rotating plate 8, is then rotatably connected to the inner wall of the baffle 3. When the hand-tightening screw 9 is tightened, it generates a pulling force on the baffle 3 towards the reagent storage box 1, ensuring that the baffle 3 fits tightly against the front of the reagent storage box 1. This effectively prevents the baffle 3 from shaking and causing the receiving block 5 and reagent tube 6 to move during equipment movement or operation, ensuring that the reagent tube 6 is stably placed inside the reagent storage box 1 and preventing reagent spillage or damage to the reagent tube 6. When it is necessary to remove or insert reagent tube 6, simply turn the hand screw 9 in the opposite direction to loosen the tension on the baffle 3, and then rotate the rotating plate 8 around the fixed axis 7 to remove the baffle 3 from the front of the reagent storage box 1, making it convenient for operators to operate on the reagent tube 6 on the receiving block 5. After the operation is completed, return the baffle 3 to its original position and turn the hand screw 9 to tighten it, which can quickly complete the installation and fixation of the baffle 3. The whole process is simple and convenient, improving work efficiency.

[0027] The overall mechanism achieves the following effect: the receiving block 5 is guided by the fixed slide rail 2 at the bottom of the reagent storage box 1. The operator pulls the handle 4 on the front of the baffle 3 to make the receiving block 5 slide outward along the fixed slide rail 2. Then, the reagent tubes 6 containing the DNA sample solution are placed into the internal holes of the receiving block 5 in sequence. The receiving block 5 is then pushed back into the reagent storage box 1 to complete the storage. When PCR amplification and other detection operations are required, and multiple reagents need to be added in sequence, the operator rotates the twisting block 103, which drives the rotating rod 102 to rotate inside the limiting block 101. When the rotating rod 102 rotates, the cam 104 fixedly mounted on it rotates accordingly. The outer surface of the cam 104 fits against the lower surface of the protrusion 105. As the stroke of the cam 104 changes, the protrusion 105 is pushed upward, thereby causing the cover plate 106 to open upward, revealing the corresponding reagent tube 6 below. Since the end of the rotating rod 102 is fixedly mounted inside the ratchet 114, and the pawl 113 is mounted on the T-plate 111 through the rotating shaft 112 and is engaged with the surface of the ratchet 114, the one-way locking effect of the ratchet 114 and the pawl 113 ensures that the rotating rod 102 can only rotate in one direction and cannot rotate back. This physically forces the operator to take the reagents in the preset order. The outer surface of the rotating rod 102 is also fixedly mounted with a cam 1010 whose initial phase position is different from that of the cam 104. As the operator continues to rotate and twist block 103, cam 1010 then contacts and lifts protrusion 1011, causing cover 1012 to open and reveal the next set of reagent tubes 6. During each opening process, the connecting spring 108 and telescopic rod 109 inside the rectangular groove 107 provide a restoring force for cover 106 and cover 1012, ensuring automatic closure after disengagement. After all reagents have been added, the operator can rotate the hand screw 9 to rotate the rotating plate 8 around the fixed shaft 7, thereby locking the baffle 3 to the front of the reagent storage box 1 to prevent the receiving block 5 from accidentally slipping out during transportation or storage. This is achieved through the pure mechanical linkage of the staggered lifting mechanism 10 and the locking mechanism 11.

[0028] The reagent storage box 1 and cover plate 106 and cover plate 2 1012 should be injection molded from low-temperature resistant and corrosion-resistant polymer materials to adapt to the storage environment and avoid material brittleness or deformation due to long-term low temperature. This also prevents corrosion of the shell by salts or organic solvents in the DNA extraction solution. The sliding contact surfaces of the fixed slide rail 2 and the receiving block 5 should be made of a material with good self-lubricating properties, ensuring sufficient fitting accuracy to prevent excessive frictional resistance from causing the baffle 3 to jam or generating metal fragments that contaminate the reagent tube 6. Cam 1 104 and Cam 2... The transmission components that come into frequent contact, such as 1010, 105, and 1011, are made of engineering plastics with excellent wear resistance or lightweight metals with surface treatment to ensure that the cam lift curve does not wear significantly during long-term use, thereby ensuring the accuracy of the cover opening sequence and stroke. The meshing surfaces of ratchet 114 and pawl 113 must have sufficient hardness and fatigue strength, and are made of stainless steel or quenched carbon steel. An appropriate amount of low-temperature grease is applied during assembly to ensure the reliability of the one-way locking function and the operation feel in low-temperature environments.

[0029] Before placing the reagent tube 6 into the receiving block 5, ensure that the cap of the reagent tube 6 is tightly closed and place it in the preset hole sequence. Simultaneously check that the handle 4 on the front of the baffle 3 is secure to prevent the reagent tube 6 from tipping over or the sample from spilling due to excessive force when pulling the receiving block 5. When rotating the block 103 to retrieve the reagent, apply force slowly and evenly. When you hear a clear click from the ratchet 114 and pawl 113, it indicates that the next locking position has been reached. Only then can you open the cover plate 106 or the cover plate 1012 to retrieve the liquid. Do not forcibly rotate in the opposite direction or use force to exceed the locking limit of the ratchet 114, as this may damage the pawl 113 or deform the rotating rod 102. After each use, promptly... Tighten the unused reagent tube 6 again and check whether the connecting spring 108 and telescopic rod 109 are properly reset. Ensure that cover plate 106 and cover plate 2012 are completely closed. Then transfer the entire reagent storage box 1 to a refrigerator or low-temperature transport box for storage. At the same time, check whether the information on the QR code label affixed to the side of the box lid is consistent with the internal sample record. Regularly clean and lubricate the moving parts such as the fixed slide rail 2, cam 104, cam 2010, ratchet 114 and pawl 113. If the sliding resistance is found to be significantly increased or the ratchet 114 makes an abnormal meshing sound, clean up any dust, ice crystals or reagent residue that may have entered in time, and add an appropriate amount of lubricant suitable for low-temperature environments to ensure the smoothness and reliability of the equipment in long-term use.

[0030] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A molecular marker-assisted breeding device, characterized in that: The reagent storage box (1) is equipped with a fixed slide rail (2) at the bottom of the inside of the reagent storage box (1). A receiving block (5) is slidably connected to the outer surface of the fixed slide rail (2). A reagent tube (6) is placed inside the receiving block (5). A baffle (3) is fixedly installed on the front of the receiving block (5). A handle (4) is fixedly installed on both sides of the front of the baffle (3). The bottom of the baffle (3) is slidably connected to the outer surface of the fixed slide rail (2). A staggered lifting mechanism (10) is installed on both sides of the reagent storage box (1). A locking mechanism (11) that converts rotational force into locking force is installed on the output shaft of the staggered lifting mechanism (10).

2. The molecular marker-assisted breeding device according to claim 1, characterized in that: The staggered lifting mechanism (10) includes a limiting block (101), a rotating rod (102), a twisting block (103), a cam (104), a protrusion (105), and a cover plate (106). The left side of the limiting block (101) is fixedly installed with the right side of the reagent storage box (1). The outer surface of the rotating rod (102) is rotatably installed with the inside of the limiting block (101). The back side of the twisting block (103) is fixedly installed with one end of the rotating rod (102). The inside of the cam (104) is fixedly installed with the outer surface of the rotating rod (102). The lower surface of the cover plate (106) is in contact with the upper surface of the reagent storage box (1). The upper surface of the protrusion (105) is fixedly installed with the lower surface of the cover plate (106). The lower surface of the protrusion (105) is in contact with the outer surface of the cam (104).

3. The molecular marker-assisted breeding device according to claim 1, characterized in that: The locking mechanism (11) includes a T-shaped plate (111), a rotating shaft (112), a pawl (113), and a ratchet (114). The left side of the T-shaped plate (111) is fixedly installed with the right side of the reagent storage box (1). The outer surface of the rotating shaft (112) is rotatably installed with the inner wall of the T-shaped plate (111). The inside of the pawl (113) is fixedly installed with the outer surface of the rotating shaft (112). The ratchet (114) is fitted onto the surface of the pawl (113).

4. The molecular marker-assisted breeding device according to claim 1, characterized in that: The reagent storage box (1) has a rectangular groove (107) on its upper surface. A connecting spring (108) is fixedly installed at the bottom of the rectangular groove (107), and a telescopic rod (109) is fixedly installed at the bottom of the rectangular groove (107).

5. The molecular marker-assisted breeding device according to claim 4, characterized in that: One end of the rectangular groove (107) and the connecting spring (108) are fixedly installed on the lower surface of the cover plate (106).

6. The molecular marker-assisted breeding device according to claim 2, characterized in that: The outer surface of the rotating rod (102) is fixedly mounted with a second cam (1010), and the initial phase position of the second cam (1010) is different from that of the first cam (104).

7. The molecular marker-assisted breeding device according to claim 6, characterized in that: The outer surface of the cam 2 (1010) is fitted with a protrusion 2 (1011), and the upper surface of the protrusion 2 (1011) is fixedly fitted with a cover plate 2 (1012). The lower surface of the cover plate 2 (1012) is fitted with the upper surface of the reagent storage box (1).

8. The molecular marker-assisted breeding device according to claim 3, characterized in that: The ratchet (114) is fixedly installed inside and on the outer surface of one end of the rotating rod (102) to prevent the rotating rod (102) from rotating back when it rotates.

9. The molecular marker-assisted breeding device according to claim 1, characterized in that: The reagent storage box (1) has a fixed shaft (7) rotatably mounted on the inner wall of the front side, and a rotating plate (8) is fixedly mounted on the outer surface of the fixed shaft (7).

10. A molecular marker-assisted breeding device according to claim 9, characterized in that: The rotating plate (8) is threaded with a hand screw (9), and the outer surface of the hand screw (9) is rotatably mounted to the inner wall of the baffle (3).

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