Low-temperature high-activity composite microbial inoculant synergistic bio-organic fertilizer

Abstract

The application discloses a kind of low-temperature high-activity composite microbial inoculant synergistic bio-organic fertilizer, by gradient low-temperature acclimation composite microbial inoculant, rotten organic matter matrix, porous anti-freezing bearing auxiliary material, microbial population synergistic coordination agent compound is made;Composite microbial inoculant is by mutagenesis acclimation low-temperature resistant bacillus subtilis, polar pseudomonas, low-temperature nitrogen-fixing frankia is compounded according to active bacteria ratio, supplemented with polyhydric alcohol coordination to construct microbial population symbiotic system, rely on porous modified zeolite water-locking bacteria, can guarantee 5~12 ℃ low temperature strain intracellular low-temperature protease, organic matter degradation enzyme activity stability, three strains realize carbon and nitrogen decomposition, nitrogen-fixing phosphorus-solubilizing synergistic metabolism;The fertilizer 5~12 ℃ environment organic matter 7d decomposition rate is greater than or equal to 68%, and strain antagonism rate is less than 7%, is suitable for northern early spring, high-cold area low-temperature farmland application, preparation process is simple, and is suitable for industrialized mass production.

Description

Technical Field

[0001] This invention belongs to the field of functional microbial organic fertilizer preparation technology, specifically relating to a low-temperature, high-activity compound microbial agent synergistic effect bio-organic fertilizer. Background Technology

[0002] Existing commercially available bio-organic fertilizers use functional microbial strains selected for use at room temperature, with an optimal metabolic activity temperature range of 20–30℃. These strains are only suitable for application in spring and summer in fields with normal temperatures, and suffer from a core technical deficiency: in early spring (5–12℃) and in consistently low-temperature environments at high altitudes, the spatial conformation of organic matter-degrading enzymes and nitrogen-fixing and phosphorus-solubilizing enzymes within the cells of these functional microorganisms becomes inactive, significantly reducing cell membrane fluidity. Simultaneously, overlapping metabolic substrates and mutual inhibition of secondary metabolites among multiple strains lead to significant antagonistic and repulsive effects between strains, preventing the formation of a synergistic metabolic system. This ultimately results in the stagnation of organic matter decomposition, the inability to release nutrients, and a complete loss of soil improvement, root-promoting, and nutrient-supplying effects. This fails to meet the urgent need for low-temperature fertilization in early spring sowing in northern regions and year-round planting in high-altitude, cold areas. Current industry-standard improvement methods only involve adding antifreeze additives, which cannot solve the coupling problem of inactivated endogenous enzymes and lack of synergistic antagonism within the microbial community. The effect of improving fertilizer efficiency at low temperatures is extremely limited, and there is no integrated solution suitable for low temperatures and synergistic microbial community development. Summary of the Invention

[0003] Addressing the technical problems raised in the background art—namely, the inactivation of metabolic enzymes in conventional functional bacteria at low temperatures of 5–12℃, antagonistic and mutually exclusive interactions among complex bacterial strains, lack of synergistic metabolic effects in the bacterial community, and significant reduction in the decomposition of organic matter in organic fertilizers and soil activation efficiency—this invention relies on the integrated combination of exclusive mutagenesis-induced cold-resistant bacterial strains, a bacterial community coordination synergistic system, and a porous bacterial preservation and antifreeze carrier to construct a low-temperature collaborative bacterial community system. This system solves the aforementioned technical problems from three dimensions: endogenous cold-resistant modification of bacterial strains, exogenous antagonistic regulation of bacterial communities, and thermal insulation and bacterial preservation of the carrier microenvironment. It achieves synergistic synergistic effects of the bacterial community at low temperatures, resulting in the stable release of organic fertilizer nutrients.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] A low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer is made from the following raw materials in parts by weight: 2.2-3.5 parts of gradient low-temperature acclimatization compound microbial agent, 62-68 parts of decomposed mushroom residue mixed with livestock and poultry organic matter, 14-19 parts of porous antifreeze modified zeolite auxiliary material, 4.5-6.2 parts of microbial community synergist, 3.0-4.8 parts of organic antifreeze protectant, and 2.8-4.0 parts of slow-release potassium humate.

[0006] In a preferred embodiment of the present invention, the gradient low-temperature acclimatization compound bacterial agent is composed of low-temperature resistant Bacillus subtilis live bacterial powder, Pseudomonas polaris live bacterial powder, and low-temperature nitrogen-fixing Frankincense live bacterial powder in a live bacterial ratio of 4:3:2. All three strains have been bred through argon plasma mutagenesis and gradient low-temperature acclimatization at 5℃, and the intracellular low-temperature cellulase activity of the strains is ≥23U / mL.

[0007] In a preferred embodiment of the present invention, the decomposed mushroom residue mixed with livestock and poultry organic matter is a product of high-temperature aerobic decomposition of king oyster mushroom residue and decomposed cow manure at a dry weight ratio of 5:4, with a total organic matter content of ≥65%.

[0008] In a preferred embodiment of the present invention, the porous antifreeze modified zeolite is a 200-mesh clinoptilolite modified by soaking in a saturated sodium bicarbonate solution for 24 hours and calcining at 420°C for 1.5 hours, with a porosity of 62% to 67%.

[0009] In a preferred embodiment of the present invention, the microbial community enhancing synergist is prepared by homogenizing D-trehalose, modified polyglutamic acid, and trisodium citrate in a weight ratio of 5:2:1.

[0010] In a preferred embodiment of the present invention, the organic antifreeze agent is an aqueous solution of konjac glucomannan with a mass concentration of 1.8%.

[0011] A preferred embodiment of the present invention is made from the following optimal weight parts of raw materials: 2.8 parts of gradient low temperature acclimatization compound microbial agent, 65 parts of decomposed mushroom residue mixed with livestock and poultry organic matter, 17 parts of porous antifreeze modified zeolite auxiliary material, 5.4 parts of microbial community synergist, 3.9 parts of organic antifreeze protectant, and 3.4 parts of slow-release potassium humate.

[0012] A method for preparing a low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer includes the following steps: Step S1, substrate pretreatment: Mix king oyster mushroom residue and fresh cow manure at a dry weight ratio of 5:4, add 0.3 parts of high-temperature composting bacteria, aerobic compost at 60℃ for 18 days, crush and pass through a 60-mesh sieve to obtain composted mushroom residue and livestock mixed organic matter for later use. Step S2, carrier modification: Immerse 200-mesh clinoptilolite in a saturated sodium bicarbonate solution for 24 hours, drain, calcine at 420℃ for 1.5 hours, and cool naturally to room temperature to obtain porous antifreeze modified zeolite additive. Step S3, preparation of adjuvants: homogenize D-trehalose, modified polyglutamic acid and trisodium citrate according to the ratio to prepare a microbial community synergist, and prepare an organic antifreeze protectant by preparing a 1.8% pure aqueous solution of konjac glucomannan. Step S4, Low-temperature mixing: Under light-proof and room-temperature conditions, mix three strains of domesticated live bacteria powder at a live bacteria ratio of 4:3:2, spray with a bacterial community synergist at 12% of the total bacterial powder mass, stir at low speed for 18 minutes to complete the surface coordination modification of the strains and eliminate the metabolic antagonism of the strains. Step S5, low-temperature granulation: The ambient temperature is controlled at 8-11℃. Well-rotted organic matter, modified zeolite, and modified compound microbial agent are added in sequence. The mixture of organic antifreeze protectant and slow-release potassium humate is sprayed at a uniform speed. After stirring and mixing, the mixture is granulated in a disc, dried at a low temperature of 40℃ with hot air, and the finished product is sieved into 2-4mm granules.

[0013] In a preferred embodiment of the present invention, in step S4, the low-speed stirring speed is 35 r / min, the relative humidity of the stirring environment is 58% to 63%, and the entire process is kept away from light to avoid ultraviolet inactivation of low-temperature strains.

[0014] In a preferred embodiment of the present invention, in step S5, the dried finished product is sealed in a light-proof container and stored at an ambient temperature of 0–25°C. The finished product has a shelf life of 6 months at room temperature, and the viable bacteria activity attenuation rate is ≤12% when applied at a low temperature of 5°C.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. Solve the problems of existing technologies: adapt to the low temperature environment of farmland of 5-12℃, stably express the endogenous cold-resistant enzymes of the strain, promote the chain-like synergistic metabolism of the microbial community, completely eliminate strain antagonism, and increase the organic matter decomposition rate from 47.3% of conventional low temperature organic fertilizer to more than 69% in 7 days, thereby improving the efficiency of low temperature fertilizer supply.

[0016] 2. The synergistic effect of the microbial community has unexpected technical effects: the three strains have differentiated functional division and coordination symbiotic system, which increases nitrogen fixation at low temperature and increases the activation of available phosphorus in the soil. It is not a superposition of the functions of a single strain, and the coupled synergistic effect cannot be achieved by conventional compound fertilizers in the industry.

[0017] 3. Suitable for industrial mass production: It can be modified to produce conventional organic fertilizer. The finished product has uniform particle size and can be applied mechanically. It has a shelf life of 6 months at room temperature and does not have the problem of large-scale inactivation of live bacteria during storage and transportation in high-altitude and cold regions.

[0018] 4. Strong ecological adaptability: The fertilizer is pH neutral and will not cause salt accumulation when applied at low temperatures. It improves compacted soil at low temperatures, enhances soil microbial community diversity, and is suitable for all scenarios of low-temperature planting of spring corn in the north, vegetables in cold and high-altitude areas, and seedlings under forests. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments or prior art, the drawings used in the description of the embodiments or prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a cross-sectional schematic diagram of a low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer provided in an embodiment of the present invention.

[0021] Figure 2 This invention provides a schematic diagram of the synergistic metabolic chain reaction mechanism of three strains in a low-temperature, high-activity compound microbial agent-enhanced bio-organic fertilizer.

[0022] Figure 3 This is a schematic diagram illustrating the preservation of live bacteria in fertilizer strains under operating conditions of 5–12°C, as provided in an embodiment of the present invention.

[0023] Figure 4 This is a flowchart illustrating a method for preparing a low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer according to an embodiment of the present invention. Detailed Implementation

[0024] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of this application.

[0025] This invention provides a low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer, made from the following raw materials in parts by weight: 2.2-3.5 parts of gradient low-temperature acclimatization compound microbial agent, 62-68 parts of decomposed mushroom residue and mixed organic matter with livestock and poultry, 14-19 parts of porous antifreeze modified zeolite additive, 4.5-6.2 parts of microbial community synergist, 3.0-4.8 parts of organic antifreeze protectant, and 2.8-4.0 parts of slow-release potassium humate; the gradient low-temperature acclimatization compound microbial agent is composed of low-temperature resistant Bacillus subtilis live bacteria powder, Pseudomonas polaris live bacteria powder, and low-temperature nitrogen-fixing Frankincense live bacteria powder in a live bacteria ratio of 4:3:2, and all three strains have been treated with argon gas, etc. Nine generations of selective breeding were performed using plasma mutagenesis followed by a 5℃ gradient low-temperature acclimatization process, resulting in intracellular low-temperature cellulase activity ≥23 U / mL. The organic matter from the decomposed mushroom residue and livestock mixture was a product of high-temperature aerobic decomposition of Pleurotus ostreatus mushroom residue and decomposed cow manure at a dry weight ratio of 5:4, with a total organic matter content ≥65%. The porous antifreeze modified zeolite was a 200-mesh clinoptilolite modified by soaking in a saturated sodium bicarbonate solution for 24 hours and calcined at 420℃ for 1.5 hours, with a porosity of 62%–67%. The microbial community synergist was a homogenized mixture of D-trehalose, modified polyglutamic acid, and trisodium citrate at a weight ratio of 5:2:1. The organic antifreeze protectant was an aqueous solution of konjac glucomannan with a mass concentration of 1.8%.

[0026] like Figure 2 and Figure 3As shown, the gradient low-temperature acclimatization compound microbial agent (2.2–3.5 parts) is the core functional component, with a precise 4:3:2 live bacteria ratio adapted to chain metabolism. Low-temperature resistant Bacillus subtilis decomposes organic fertilizer crude fiber and lignin macromolecular organic matter, producing small-molecule carbon sources; Pseudomonas polaris utilizes these small-molecule carbon sources to activate insoluble phosphorus in the soil, while simultaneously secreting extracellular polysaccharides to encapsulate the bacterial cells for freeze resistance; and Frankelbrood, a low-temperature nitrogen-fixing bacterium, relies on the carbon metabolism energy of the first two strains to complete low-temperature biological nitrogen fixation. The three strains have complementary substrate supply and demand, exhibiting no antagonism after coordination, and can autonomously complete closed-loop metabolism at 5°C. Plasma mutagenesis modifies the enzyme gene loci of the strains, enhancing the structural stability of the low-temperature enzymes. Compared to unacclimatized strains, the low-temperature enzyme activity is increased by 72%, achieving macromolecular organic matter degradation and carbon production by Bacillus subtilis, phosphorus solubilization and freeze resistance by Pseudomonas polaris, low-temperature nitrogen fixation by Frankelbrood, and enrichment of readily available nutrients in the soil.

[0027] In the mixed organic matter of decomposed mushroom residue and livestock (62-68 parts), the woody fiber pores of king oyster mushroom residue are rich, which can provide a colonization site for the strain. Decomposed cow manure provides a readily available nitrogen source. The carbon-nitrogen ratio of 5:4 is maintained at 22:1, which is suitable for the carbon and nitrogen requirements of low-temperature microbial metabolism and avoids carbon-nitrogen imbalance leading to microbial dormancy. After decomposition, there are no aseptic bacteria, and exogenous bacteria are prevented from competing for nutrients and inhibiting the activity of functional bacteria.

[0028] In the porous antifreeze modified zeolite additive (14-19 parts), sodium bicarbonate modification opens up the closed micropores of zeolite, increasing the porosity to over 62%. The micropores can store organic antifreeze liquid and lock in shallow soil moisture, forming a constant temperature microenvironment inside the particles and isolating them from the impact of low temperatures of 5-12℃ from the outside. At the same time, the zeolite charge adsorption fixes live bacteria, preventing the loss of bacteria during granulation and storage, and increasing the retention time of live bacteria in the fertilizer.

[0029] refer to Figure 1 In the microbial community synergist (4.5-6.2 parts), D-trehalose 1 binds to the bacterial cell membrane and maintains the low-temperature phospholipid bilayer structure; modified polyglutamic acid 2 crosslinks the surface of the three strains and blocks the diffusion of antibacterial substances; trisodium citrate 3 adjusts the pH of the particle microenvironment to neutral and adapts to the optimal growth pH of the three strains. The three work synergistically to eliminate metabolic antagonism among the strains and construct a symbiotic metabolic system, which is the core synergistic synergistic agent of this invention.

[0030] In the organic antifreeze protectant and slow-release potassium humate 4, 1.8% konjac glucomannan fills the gaps in the bacterial cell membrane, lowers the intracellular freezing point, and prevents intracellular ice crystals from piercing the bacterial cells at low temperatures; slow-release potassium humate slowly enhances soil colloidal activity, assists in repairing the roots of low-temperature crops, simultaneously empowers the metabolism of the bacterial community, and maintains the long-term stability of the bacterial community activity.

[0031] Preferably, it is made from the following raw materials in optimal weight proportions: 2.8 parts of gradient low-temperature acclimatization compound microbial agent, 65 parts of decomposed mushroom residue mixed with livestock and poultry organic matter, 17 parts of porous antifreeze modified zeolite adjuvant, 5.4 parts of microbial community synergist, 3.9 parts of organic antifreeze protectant, and 3.4 parts of slow-release potassium humate. The optimal ratio of each component is defined to balance raw material costs, low-temperature viable microbial activity, and organic matter decomposition efficiency, making it suitable for large-scale field application.

[0032] The minimum proliferation temperature of *Bacillus subtilis* is 5℃, with a viable bacterial count ≥200 million CFU / g at room temperature and a viable bacterial survival rate ≥81% after 72 hours of incubation at 5℃. *Pseudomonas polaris* can autonomously secrete extracellular cryopolysaccharides at 5℃, with a strain antagonistic inhibition index ≤0.06. *Frankella asiatica*, a cryogenic nitrogen-fixing bacterium, has a nitrogenase activity ≥11 nmol / (mL·h) at 5℃ and exhibits no antibacterial activity from its metabolites.

[0033] The finished fertilizer has a particle size of 2-4 mm, a moisture content of 11%-14%, an effective viable bacteria count of ≥220 million CFU / g, and a pH value of 7.0-7.8.

[0034] Under constant temperature culture conditions of 5–12℃, the organic matter decomposition rate of fertilizer after 7 days is ≥68%, and the pairwise antagonism rate of compound strains is ≤7%.

[0035] like Figure 4 As shown, a method for preparing a low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer is characterized by comprising the following steps: Step S1, substrate pretreatment: Mix king oyster mushroom residue and fresh cow manure at a dry weight ratio of 5:4, add 0.3 parts of high-temperature composting bacteria, aerobic compost at 60℃ for 18 days, crush and pass through a 60-mesh sieve to obtain composted mushroom residue and livestock mixed organic matter for later use. Step S2, carrier modification: Immerse 200-mesh clinoptilolite in a saturated sodium bicarbonate solution for 24 hours, drain, calcine at 420℃ for 1.5 hours, and cool naturally to room temperature to obtain porous antifreeze modified zeolite additive. Step S3, preparation of adjuvants: homogenize D-trehalose, modified polyglutamic acid and trisodium citrate according to the ratio to prepare a microbial community synergist, and prepare an organic antifreeze protectant by preparing a 1.8% pure aqueous solution of konjac glucomannan. Step S4, Low-temperature mixing: Under light-proof and room-temperature conditions, mix three strains of domesticated live bacteria powder at a live bacteria ratio of 4:3:2, spray with a bacterial community synergist at 12% of the total bacterial powder mass, stir at low speed for 18 minutes to complete the surface coordination modification of the strains and eliminate the metabolic antagonism of the strains. Step S5, low-temperature granulation: The ambient temperature is controlled at 8-11℃. Well-rotted organic matter, modified zeolite, and modified compound microbial agent are added in sequence. The mixture of organic antifreeze protectant and slow-release potassium humate is sprayed at a uniform speed. After stirring and mixing, the mixture is granulated in a disc, dried at a low temperature of 40℃ with hot air, and the finished product is sieved into 2-4mm granules.

[0036] Preferably, in step S4, the low-speed stirring speed is 35 r / min, the relative humidity of the stirring environment is 58%–63%, and the entire process is conducted in the dark to avoid ultraviolet light inactivation of the low-temperature strains. In step S5, after drying, the finished product is sealed in a light-proof container and stored at a temperature of 0–25°C. The finished product has a shelf life of 6 months at room temperature, and the viable bacteria activity attenuation rate is ≤12% when applied at a low temperature of 5°C.

[0037] This invention employs argon plasma mutagenesis at 80W power + 5℃ gradient cooling for 9 generations of directional domestication to select three exclusive cold-resistant strains. These strains are then directionally enriched with exclusive functions of low-temperature cellulose degradation, low-temperature phosphorus solubilization, and low-temperature nitrogen fixation, resulting in differentiated metabolic pathways and reducing competition for metabolic substrates from the source.

[0038] By employing a D-trehalose and modified polyglutamic acid coordination coating structure, it can be directionally bound to the surface protein sites of three strains, blocking the contact of secondary antibacterial metabolites of the strains. This reduces the antagonistic rate between natural strains from more than 29% to less than 7%, achieving a chain-like synergistic metabolism of "degradation-phosphorus solubilization-nitrogen fixation" and achieving a coupled synergistic effect.

[0039] Sodium bicarbonate-modified clinoptilolite creates a porous, heat-insulating microenvironment that adsorbs and retains antifreeze polysaccharides and nutrients. It constructs a stable micro-domain at 14–18°C inside fertilizer granules to counteract low-temperature stress in the field and ensure the spatial conformation stability of intracellular enzymes in the strain. Combined with konjac glucomannan to repair low-temperature cell membranes, the strain's activity is protected through a synergistic effect from both inside and outside, overcoming the limitations of conventional methods that only involve adding antifreeze externally.

[0040] Example 1 Raw material weight parts: 2.8 parts of gradient low-temperature acclimatization compound microbial agent, 65 parts of decomposed mushroom residue and livestock and poultry mixed organic matter, 17 parts of porous antifreeze modified zeolite auxiliary material, 5.4 parts of microbial community synergist, 3.9 parts of organic antifreeze protectant, and 3.4 parts of slow-release potassium humate; the ratio of live microbial strains to Bacillus subtilis: Pseudomonas polaris: Frankensteinia coli = 4:3:2; prepared according to the process of claims 8-10: step S1, mushroom residue and cow dung are mixed in a 5:4 ratio, and 0.3 parts of high-temperature composting microbial agent is added. The zeolite was fermented at 60℃ for 18 days and then pulverized into 60-mesh powder for later use. In step S2, 200-mesh clinoptilolite was soaked in sodium bicarbonate for 24 hours and calcined at 420℃ for 1.5 hours to obtain modified zeolite. In step S3, a coordination agent and a 1.8% konjac glucomannan antifreeze solution were prepared. In step S4, the strain was stirred at 35 r / min in the dark for 18 minutes to complete the coordination modification. In step S5, the mixture was mixed in a closed loop at 9℃, granulated in a disc, dried at 40℃, and sieved to obtain 2-4 mm particles before being sealed and packaged. The finished product had an effective viable bacteria count of 270 million CFU / g, a moisture content of 12.3%, an organic matter decomposition rate of 69.4% at 5-12℃ for 7 days, and a strain antagonism rate of 6.1%.

[0041] Comparative Example 1 (commercially available conventional compound bio-organic fertilizer, otherwise the same as in Example 1) The synergistic agent for bacterial community enhancement was removed and replaced with an equal amount of pure water. The remaining raw materials and processes remained unchanged. The antagonistic rate of the finished bacterial strains was 21.7%, the organic matter decomposition rate was 47.3% after 7 days at 5-12℃, and there was no synergistic metabolic effect of the bacterial community.

[0042] Comparative Example 2 (a combination of unacclimated, room-temperature strains; all other aspects are the same as in Example 1). The strains were replaced with three commercially available strains of the same viable bacterial content at room temperature. No plasma low-temperature acclimation was performed. The other raw materials and processes remained unchanged. The viable bacterial retention rate was 41.6% after 72 hours at 5℃, the enzyme activity was significantly deactivated at low temperature, and the organic matter decomposition rate was 52.5% after 7 days.

[0043] Comparative test data:

[0044] Experimental conclusion: Conventional methods can only slightly improve the freeze resistance of strains and cannot solve the core problems of bacterial antagonism and lack of synergistic metabolism. This invention achieves synergistic effects that exceed those of conventional technologies in the industry by combining bacterial mutagenesis, coordination aids and modified carriers in a three-in-one combination.

[0045] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A low-temperature, high-activity compound microbial agent synergistic effect bio-organic fertilizer, characterized in that, It is made from the following raw materials in parts by weight: 2.2-3.5 parts of gradient low-temperature acclimatization compound microbial agent, 62-68 parts of decomposed mushroom residue mixed with livestock and poultry organic matter, 14-19 parts of porous antifreeze modified zeolite auxiliary material, 4.5-6.2 parts of microbial community synergist, 3.0-4.8 parts of organic antifreeze protectant, and 2.8-4.0 parts of slow-release potassium humate.

2. The low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer according to claim 1, characterized in that, The gradient low-temperature domestication compound bacterial agent is composed of live powders of Bacillus subtilis, Pseudomonas polaris, and Frankincense, which are resistant to low temperatures, in a live bacterial ratio of 4:3:

2. All three strains have been bred through argon plasma mutagenesis and gradient low-temperature domestication at 5℃. The intracellular low-temperature cellulase activity of the strains is ≥23U / mL.

3. The low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer according to claim 2, characterized in that, The decomposed mushroom residue mixed with livestock and poultry organic matter is a product of high-temperature aerobic decomposition of king oyster mushroom residue and decomposed cow manure at a dry weight ratio of 5:4, with a total organic matter content of ≥65%.

4. The low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer according to claim 3, characterized in that, The porous antifreeze modified zeolite is a 200-mesh clinoptilolite that has been modified by soaking in a saturated sodium bicarbonate solution for 24 hours and calcined at 420℃ for 1.5 hours, with a porosity of 62% to 67%.

5. The low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer according to claim 4, characterized in that, The microbial community synergist is prepared by homogenizing D-trehalose, modified polyglutamic acid, and trisodium citrate in a weight ratio of 5:2:

1.

6. The low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer according to claim 5, characterized in that, The organic antifreeze protectant is an aqueous solution of konjac glucomannan with a mass concentration of 1.8%.

7. The low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer according to claim 6, characterized in that, It is made from the following optimal weight parts of raw materials: 2.8 parts of gradient low temperature acclimatization compound microbial agent, 65 parts of decomposed mushroom residue mixed with livestock and poultry organic matter, 17 parts of porous antifreeze modified zeolite auxiliary material, 5.4 parts of microbial community synergist, 3.9 parts of organic antifreeze protectant, and 3.4 parts of slow-release potassium humate.

8. A method for preparing the low-temperature, high-activity compound microbial agent synergistic bio-organic fertilizer according to claim 7, characterized in that, Includes the following steps: Step S1, substrate pretreatment: Mix king oyster mushroom residue and fresh cow manure at a dry weight ratio of 5:4, add 0.3 parts of high-temperature composting bacteria, aerobic compost at 60℃ for 18 days, crush and pass through a 60-mesh sieve to obtain composted mushroom residue and livestock mixed organic matter for later use. Step S2, carrier modification: Immerse 200-mesh clinoptilolite in a saturated sodium bicarbonate solution for 24 hours, drain, calcine at 420℃ for 1.5 hours, and cool naturally to room temperature to obtain porous antifreeze modified zeolite additive. Step S3, preparation of adjuvants: homogenize D-trehalose, modified polyglutamic acid and trisodium citrate according to the ratio to prepare a microbial community synergist, and prepare an organic antifreeze protectant by preparing a 1.8% pure aqueous solution of konjac glucomannan. Step S4, Low-temperature mixing: Under light-proof and room-temperature conditions, mix three strains of domesticated live bacteria powder at a live bacteria ratio of 4:3:2, spray with a bacterial community synergist at 12% of the total bacterial powder mass, stir at low speed for 18 minutes to complete the surface coordination modification of the strains and eliminate the metabolic antagonism of the strains. Step S5, low-temperature granulation: The ambient temperature is controlled at 8-11℃. Well-rotted organic matter, modified zeolite, and modified compound microbial agent are added in sequence. The mixture of organic antifreeze protectant and slow-release potassium humate is sprayed at a uniform speed. After stirring and mixing, the mixture is granulated in a disc, dried at a low temperature of 40℃ with hot air, and the finished product is sieved into 2-4mm granules.

9. The method for synergistic enhancement of low-temperature, high-activity compound microbial agents and bio-organic fertilizers according to claim 8, characterized in that, In step S4, the low-speed stirring speed is 35 r / min, the relative humidity of the stirring environment is 58% to 63%, and the entire process is kept away from light to avoid ultraviolet inactivation of the low-temperature strain.

10. The method for synergistic enhancement of low-temperature, high-activity compound microbial agents and bio-organic fertilizers according to claim 8, characterized in that, In step S5, the dried product is sealed in a light-proof container and stored at an ambient temperature of 0–25°C. The product has a shelf life of 6 months at room temperature, and the viable bacteria activity attenuation rate is ≤12% when applied at a low temperature of 5°C.

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