Method for promoting straw decomposition by recruiting azotobacter with lipopeptide

Abstract

The application provides a method for promoting straw decomposition by recruiting azotobacter with lipopeptide, and belongs to the technical field of agricultural soil improvement and straw resource utilization. The application takes three functional biological synergistic stimulants as core preparations, recruits soil azotobacter by targeting lipopeptide signal molecules, and matches sugar lipid active components to supplement carbon and promote bacteria and activate soil trace elements. The application effectively solves the technical pain points of the slow decomposition rate of straw returned to field, the small number of azotobacter, the breeding of soil harmful bacteria, and the influence of field environment stress on decomposition efficiency. The application does not need to add azotobacter agents, activates soil indigenous azotobacter groups in situ by relying on natural biological active substances, can significantly improve the straw organic matter decomposition efficiency and the soil biological nitrogen fixation amount, improves the microecology of the continuous cropping obstacle soil, reduces the amount of chemical fertilizers and pesticides, is green, pollution-free, and drug-resistant, and is suitable for the straw returned to field scene of wheat, corn, cotton and other major crops, and has high application and popularization value.

Description

Technical Field

[0001] This invention relates to the field of agricultural soil improvement and straw resource utilization technology, and more specifically, to a method for recruiting nitrogen-fixing bacteria using lipopeptides to promote straw decomposition. Background Technology

[0002] Returning crop straw to the field is a core technology for green agricultural production, recycling straw resources, and improving soil organic matter. It can effectively improve soil structure, enrich soil fertility, and reduce environmental pollution caused by straw burning. However, current straw return technology generally has many shortcomings: First, the lignocellulose structure of straw is stable, and its natural decomposition rate is slow. Straw returned to the field in autumn and winter is prone to incomplete decomposition during winter, affecting subsequent sowing and crop seedling growth. Second, long-term continuous cropping in farmland soil leads to microecological imbalance, with low numbers and low activity of indigenous nitrogen-fixing bacteria and decomposing bacteria, resulting in weak soil biological nitrogen fixation capacity and low efficiency of straw nutrient release. Third, the large-scale proliferation of harmful oomycetes such as Phytophthora and Pythium in the soil competes with beneficial microorganisms for nutrients, inhibiting the proliferation of nitrogen-fixing bacteria and decomposing bacteria. Fourth, field stresses such as drought and low temperature further reduce microbial activity and significantly delay the straw decomposition process.

[0003] Existing technologies mostly accelerate straw decomposition by adding exogenous decomposing bacteria, nitrogen-fixing bacteria, or chemical decomposing agents. However, exogenous agents are easily rejected by the local soil environment, resulting in low colonization rates and short-lasting effects. Chemical agents can easily cause soil compaction, microecological damage, and residual pollution, and cannot simultaneously achieve the multiple effects of nitrogen-fixing bacteria activation, soil improvement, and stable yield under stress. To address the shortcomings of existing technologies, this paper proposes a method that utilizes lipopeptides to recruit nitrogen-fixing bacteria to promote straw decomposition. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of existing straw return-to-field decomposition technologies, such as slow decomposition rate, weak nitrogen fixation capacity, microecological imbalance, poor stress resistance, chemical pollution, and low colonization rate of exogenous microbial agents. This invention provides a method for promoting straw decomposition by utilizing lipopeptides to recruit nitrogen-fixing bacteria. Relying on natural bioactive lipopeptides and glycolipid components, it recruits and activates native nitrogen-fixing bacteria in the soil in situ, optimizes the rhizosphere microecology, stabilizes the field environment, and efficiently accelerates straw decomposition, achieving multiple benefits including soil fertilization, enhanced nitrogen fixation, and green reduction of pesticides and fertilizers.

[0005] In order to achieve the above-mentioned invention objectives, the present invention provides the following technical solution: a method for recruiting nitrogen-fixing bacteria using lipopeptides to promote straw decomposition, step 1, straw return to the field treatment: after harvest, the crop straw in the field is crushed, evenly spread on the field surface and turned into the topsoil, keeping the topsoil moist, and constructing a soil microenvironment suitable for the colonization and reproduction of nitrogen-fixing bacteria and straw decomposition microorganisms. Step 2, Selection of drug components: A bio-enhancing stimulant system is selected for application. The bio-enhancing stimulant system includes at least one of CBN-601SF, CBN-602RL, and CBN-603BD, wherein CBN-603BD contains a lipopeptide signaling molecule for targeted recruitment of soil nitrogen-fixing bacteria. Step 3, Precise preparation of the drug solution: Dilute each drug component step by step with clean water, following the principle of preparing and using immediately. Finally, add the active components such as lipopeptides and glycolipids and stir evenly to avoid foaming. Do not mix with strong acidic materials with pH < 4 or strong alkaline materials with pH > 9. Step 4, First targeted application: Within 1 to 3 days after the straw is turned back into the field, apply the prepared pesticide solution evenly into the straw-returned soil layer using drip irrigation or flood irrigation, and use the active components of lipopeptides and glycolipids to quickly wet the interface between the straw and the soil. Step 5, Periodic application and maintenance: Apply 2-3 times in a 7-10 day cycle. The total amount of the biological stimulant system stock solution applied per acre per application is 1-2 kg to stabilize the activity of beneficial bacteria in the soil. Step 6, Pipeline cleaning: After each drip irrigation application, rinse the drip irrigation pipeline with clean water for 10-15 minutes to prevent pesticide residue from clumping and clogging the pipeline; Step 7, Soil microecological activation: CBN-602RL glycolipid components provide nitrogen-fixing bacteria with a usable rhizosphere carbon source, chelate and activate soil trace elements such as calcium, magnesium, and iron, while inhibiting harmful soil oomycete flora and reducing competitive inhibition by harmful microorganisms; Step 8: Maintaining the homeostasis of the decomposition environment: Activating plant stress resistance pathways through CBN-603BD lipopeptide signaling molecules, regulating soil and plant water transpiration, maintaining stable soil moisture throughout the straw decomposition process, and continuously ensuring the proliferation of nitrogen-fixing bacteria and the decomposition efficiency of straw organic matter.

[0006] As a preferred technical solution of the present invention, the CBN-603BD is an active preparation containing micromolar lipopeptide signaling molecules, with an effective ingredient concentration of 50g / L. The effective working concentration in straw-returned soil is controlled at 20-30mg / L, which can precisely activate the jasmonic acid / ethylene and abscisic acid pathways, induce systemic stress resistance in plants, and reduce water transpiration by 30%-40%.

[0007] As a preferred technical solution of the present invention, the CBN-602RL is a rhizosphere microecological activator containing specific structural glycolipid bioactive substances, with an effective ingredient concentration of 50g / L and an effective working concentration of 50-100mg / L in straw-returned soil. It can serve as a dedicated carbon source for the proliferation of rhizosphere nitrogen-fixing bacteria and can also effectively inhibit harmful oomycete flora such as Phytophthora and Pythium.

[0008] As a preferred technical solution of the present invention, CBN-603BD and CBN-602RL are applied in combination, with the final concentration ratio of the two liquids being 1:2 to 1:5. After the combination, they are applied to the soil using an integrated water and fertilizer drip irrigation method, which synergistically achieves the recruitment and proliferation of nitrogen-fixing bacteria and the rapid decomposition of straw.

[0009] As a preferred technical solution of the present invention, the CBN-601SF contains fermentation-derived surfactantin analog lipopeptide active substance with an effective ingredient concentration of 5g / L and an effective application concentration of 30-50mg / L, which can reduce the surface tension of the pesticide solution at the soil-straw interface to below 30mN / m, and significantly improve the wetting, spreading and penetration ability of the pesticide solution.

[0010] As a preferred technical solution of the present invention, the bio-enhancing stimulant system can be mixed with water-soluble fertilizers, micronutrient fertilizers, and Bacillus subtilis-based biological pesticides. When mixing, the fertilizer or pesticide is diluted first, and the bio-enhancing stimulant component is added last.

[0011] As a preferred technical solution of the present invention, the application of pesticides is carried out in the early morning or late evening when there is no strong light and high temperature. The soil is kept moist during the application of pesticides, which is conducive to the diffusion of active components and the adsorption and colonization of nitrogen-fixing bacteria and decomposing bacteria on the surface of straw.

[0012] As a preferred technical solution of the present invention, the CBN-602RL activates soil trace elements such as calcium, magnesium and iron through chelation, releases soil nutrient fixation, and provides sufficient nutrient substrate for the metabolic proliferation of nitrogen-fixing bacteria and the microbial decomposition of straw organic matter.

[0013] As a preferred technical solution of the present invention, the CBN-603BD is a non-hormonal active preparation that does not interfere with plant growth. After a single application, it can maintain the stress resistance of the soil and plants for 2 to 3 weeks and stabilize the microenvironment for straw decomposition.

[0014] As a preferred technical solution of the present invention, the whole method achieves improved straw decomposition efficiency and increases soil biological nitrogen fixation, and improves the soil microecology through multiple synergistic effects of recruiting nitrogen-fixing bacteria colonization by lipopeptide signaling molecules, supplementing carbon and promoting bacteria by glycolipids, activating soil nutrients, and stabilizing the soil microenvironment.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention utilizes CBN-603BD lipopeptide signaling molecules to target and recruit native nitrogen-fixing bacteria in the soil, and combines it with CBN-602RL glycolipid components to provide a dedicated carbon source. There is no need to artificially add nitrogen-fixing bacteria or decomposing agents, which completely solves the problems of low colonization rate, easy inactivation and short duration of effect of exogenous agents, and significantly reduces the application cost.

[0016] Through the synergistic effects of six mechanisms—lipopeptide signaling to promote bacterial growth, glycolipid carbon supplementation and proliferation, trace element activation, surface activity enhancement, harmful bacteria inhibition, and stress homeostasis regulation—the formulation rapidly breaks down the lignocellulose structure of straw, significantly increasing the straw decomposition rate and soil biological nitrogen fixation. All formulations are derived from bio-fermentation active substances, are non-hormonal, residue-free, and do not induce drug resistance. They can effectively reduce the use of chemical fertilizers and pesticides by 10%–20%, improve soil aggregate structure, and alleviate continuous cropping obstacles, soil salinity, and compaction problems.

[0017] By activating crop stress resistance pathways and reducing soil moisture evaporation, this technology can maintain soil microecological stability under adverse conditions such as drought and low temperatures, ensuring continuous straw decomposition and adapting to straw return operations in different regions and climatic conditions. The entire process has clear steps and precise parameters, is compatible with mainstream field operation modes such as fertigation, drip irrigation, and flood irrigation, and is suitable for various crops such as wheat, corn, cotton, and vegetables. It is highly practical and suitable for large-scale farmland promotion and application. Attached Figure Description

[0018] Figure 1 A flowchart illustrating the function and dosage data of the bio-enhancing stimulant provided by this invention. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0020] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely illustrates some embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention. It should be noted that, in the absence of conflict, the embodiments and features and technical solutions in the embodiments of the present invention can be combined with each other. It should be noted that similar reference numerals and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0021] Example 1: A method for recruiting nitrogen-fixing bacteria using lipopeptides to promote straw decomposition Step 1, straw return to the field: After the corn harvest, crush the corn stalks in the field to 3-5cm, spread them evenly on the field surface, turn them over to the 15-20cm topsoil layer, and irrigate to keep the soil moist. Step 2, reagent selection: CBN-603BD and CBN-602RL compound system are selected, with a final concentration ratio of 1:3; Step 3, Preparation of the solution: Dilute with clean water in steps. First, dilute CBN-602RL and stir well. Then dilute CBN-603BD and stir well. Prepare and use immediately. The pH of the solution should be controlled between 5 and 8. Step 4, First application: Two days after straw is returned to the field, apply the pesticide by drip irrigation, using 1.5 kg of compound stock solution per acre, with a working concentration of 25 mg / L for CBN-603BD and 80 mg / L for CBN-602RL; Step 5, Periodic application: Apply the medicine once every 7 days, for a total of 2 applications; Step 6, Pipeline cleaning: After each drip irrigation, flush the pipeline with clean water for 12 minutes; Step 7, Microecological Activation: Relying on CBN-602RL to activate soil calcium, magnesium and iron elements, inhibit soil Pythium and Phytophthora communities, and provide carbon source and nutrients for the proliferation of nitrogen-fixing bacteria; Step 8: Environmental homeostasis regulation: Activate crop stress resistance pathways through CBN-603BD, reduce field water evapotranspiration, and maintain stable soil moisture.

[0022] Experimental results: Compared with conventional straw return to the field, this embodiment increased the straw decomposition rate by 28.6%, the number of nitrogen-fixing bacteria in the soil by 42.3%, the soil organic matter content by 16.5%, and there were no soil-borne diseases, while the amount of chemical fertilizer used was reduced by 18%.

[0023] Example 2: A method for recruiting nitrogen-fixing bacteria using lipopeptides to promote straw decomposition Step 1, straw return to the field: After wheat harvest, the straw is crushed and buryed to keep the topsoil moist; Step 2, Drug Selection and Combination: Simultaneously select CBN-601SF, CBN-602RL, and CBN-603BD in combination; Step 3, Preparation of the drug solution: Dilute in steps, and finally add the lipopeptide and glycolipid components, and stir well; Step 4, First application: One day after straw is returned to the field, apply pesticides by flood irrigation. Apply 1.2 kg of the original solution per mu, with a working concentration of 40 mg / L for CBN-601SF, 60 mg / L for CBN-602RL, and 22 mg / L for CBN-603BD. Step 5, Periodic Reapplication: Apply the medicine once every 10 days, for a total of 2 applications; Step 6, Pipeline cleaning: Rinse the pipeline with clean water for 10 minutes after drip irrigation; Step 7, Microecological Activation: Activate soil trace elements, inhibit harmful oomycete flora, and promote the proliferation of nitrogen-fixing bacteria; Step 8: Environmental homeostasis regulation: Maintain stable soil temperature and humidity to ensure the continuous activity of microorganisms.

[0024] Experimental results: The decomposition rate of wheat straw was significantly improved, and the straw was basically completely decomposed in 20 days. Soil biological nitrogen fixation increased by 35.2%, effectively preventing the occurrence of powdery mildew and root rot in the field, and reducing pesticide use by 35%.

[0025] Comparative example: Conventional straw return to field treatment The traditional straw crushing and burying method was adopted, without the application of any bio-enhancing stimulants, and conventional field water and fertilizer management was used. The results showed that the natural decomposition rate of straw was slow, with a large amount of straw residue remaining after 30 days. The number of nitrogen-fixing bacteria in the soil was low, the soil was compacted and slightly salinized, the incidence of soil-borne diseases was high, and the input of chemical fertilizers and pesticides was large.

[0026] The usage and effects of the bio-enhancing stimulant CBN-602RL: The following are feasible solutions based on application scenarios.

[0027] I. Fertilizer Enhancement (Most commonly used, increasing utilization rate by 20%–30%) 1. Combine with NPK fertilizer (for fertigation / drip irrigation) Principle: It breaks down phosphorus and potassium fixation, promotes nitrogen adsorption, and reduces leaching / volatilization.

[0028] Ratio: 50% rhamnolipin: NPK water-soluble fertilizer = 1:50–1:100 (by weight).

[0029] Concentration: The effective concentration of rhamnolipin after dilution is 50–100 mg / L.

[0030] Usage: Apply by fertigation / drip irrigation during the seedling and fruit expansion stages, once every 7–10 days, for 2–3 consecutive applications.

[0031] Effects: Fertilizer utilization rate increases by 20%–30%, chlorophyll content increases by 15%–20%, and fruit expansion is advanced by 3–5 days.

[0032] 2. Combined with micronutrients (calcium, magnesium, iron, zinc, boron) (foliar / drip irrigation) Principle: Chelates trace elements, prevents oxidation / adsorption, and improves mobility.

[0033] Ratio: Rhamnose glycolipids: Micronutrients = 1:20–1:50.

[0034] Concentration: 20–50 mg / L for foliar spraying; 50–100 mg / L for drip irrigation.

[0035] Application: Foliar spray during nutrient deficiency, flowering, and young fruit stages, once every 5–7 days, for a total of 2 times.

[0036] Effects: Prevents nutrient deficiencies, improves fruit firmness / coloring, and reduces physiological diseases.

[0037] 3. Foliar fertilizer adjuvants (single / compound) Principle: Reduce surface tension, break down the wax layer, and allow the fertilizer solution to spread and penetrate.

[0038] Concentration: 10–50 mg / L (pure product).

[0039] Usage: Mix with foliar fertilizer / pesticides for spraying, and use immediately after preparation.

[0040] Effects: Reduces fertilizer runoff and increases absorption efficiency by 30% or more.

[0041] II. Soil improvement (compactment / saline-alkali / continuous cropping obstacles) 1. Soil conditioning (drip irrigation / furrow application) Concentration: 100–200 mg / L (pure product), dosage per mu: 45–90 g.

[0042] Usage: Apply as a drip irrigation during land preparation / seedling stage, or apply in furrows with organic fertilizer.

[0043] effect: It reduces bulk density, promotes agglomeration, and improves air permeability and water retention.

[0044] Chelating Na + / Heavy metals, to alleviate salt and alkali / heavy metal stress.

[0045] It activates beneficial bacteria (phosphorus solubilization and nitrogen fixation) and alleviates continuous cropping obstacles.

[0046] 2. Specifically for saline-alkali land Solution: Rhamnose lipolipin 50–100 mg / L plus potassium humate and calcium fertilizer, drip irrigation / flood irrigation.

[0047] Effects: Yields of salt-tolerant crops such as cotton increased by 10%–15%.

[0048] III. Biological control (bacteriostatic / insecticide, green and safe) 1. Fungal diseases (powdery mildew / downy mildew / gray mold / root rot) Concentration: 50–200 mg / L (pure product).

[0049] Usage: Spray on leaves before / in the early stage of disease, once every 7 days, 2-3 times; drench roots to prevent root rot.

[0050] Mechanism: It disrupts the cell membrane of pathogens and inhibits spore germination.

[0051] 2. Piercing-sucking pests (aphids / planthoppers / tetramites) Concentration: 100–300 mg / L.

[0052] Usage: Spray on the leaves, focusing on the underside of the leaves.

[0053] Effects: Contact killing combined with food refusal, lasting 7–10 days; can be combined with matrine / azalea.

[0054] 3. Seed treatment (seed dressing / seed soaking) Concentration: 10–50 mg / L for soaking seeds for 2–4 hours, or for seed dressing (0.1%–0.5% of seed weight).

[0055] Effects: Promotes germination, prevents seed-borne diseases, and strengthens seedlings.

[0056] IV. Promoting Growth and Resisting Stress (Seedling Stage / Stage of Adversity) 1. Promote root growth and seedling development (seedling stage / transplanting) Concentration: 5–20 mg / L (low concentration promotes growth).

[0057] Usage: root soaking / root drenching / foliar spraying.

[0058] Effects: Root fresh weight increases by 20%–30%, more lateral roots are produced, and seedlings recover quickly.

[0059] 2. Resistance to adverse conditions (drought / low temperature / high temperature) Concentration: 20–50 mg / L.

[0060] Usage: Apply as a foliar spray before / during adverse conditions, once every 7 days, for a total of 2 times.

[0061] Effects: Reduces transpiration, improves water retention, and increases yield retention by 15%–25%.

[0062] V. Recommended Programs for Different Crops (Implementation Version) 1. Vegetables (tomatoes / cucumbers / cabbage) Seedling stage: drench roots with 5–10 mg / L to promote root growth and prevent damping-off.

[0063] During the growing season: 50 mg / L plus macronutrients, drip irrigation once every 10 days.

[0064] Flowering / Fruit Expansion: Apply 30 mg / L of boron and calcium to the leaves once every 7 days.

[0065] Disease prevention: 100 mg / L for prevention of powdery mildew / downy mildew.

[0066] 2. Fruit trees (apple / citrus / grape) For budding / pre-flowering: Add 20 mg / L of boron and zinc to promote flowering.

[0067] For young fruit / expanding fruit: Add calcium fertilizer at a concentration of 50 mg / L to prevent fruit cracking and increase firmness.

[0068] Post-harvest: Drench the roots with 100 mg / L to restore tree vigor.

[0069] Results: Apple yield increased by 6%–8.8%, with better coloring and increased single fruit weight.

[0070] 3. Field crops (wheat / corn / cotton) Seed dressing: Soak seeds in 10 mg / L solution, or dress seeds with 0.2% of the seed weight.

[0071] Jointing / booting stage: 45 g / mu (pure product) drip irrigation / spraying to promote tillering and increase grain size.

[0072] Grouting: Add 50 mg / L potassium dihydrogen phosphate to prevent premature aging and increase the thousand-grain weight.

[0073] VI. Apply key principles (avoiding pitfalls and increasing efficiency) Contraindications of compound preparation It can be mixed with: water-soluble fertilizers, micronutrient fertilizers, and biological pesticides (matrine / azapine / Bacillus subtilis).

[0074] Caution should be exercised when mixing with: strongly acidic (pH<4), strongly alkaline (pH>9), or copper preparations (which are prone to flocculation).

[0075] Recommendation: Prepare and use immediately. Dilute rhamnolipin first, then add other medications.

[0076] Timing of application Leaves: Avoid high temperatures and strong sunlight in the early morning / evening to reduce evaporation.

[0077] Soil: Apply when the soil is moist to facilitate diffusion and root absorption.

[0078] Purity and dosage form Preferred: High purity (≥90%), liquid / soluble powder, low impurities, and stable effect.

[0079] Avoid: crude fermentation liquid (high impurities, unstable results).

[0080] VII. Comprehensive Efficiency Enhancement Plan (Maximizing Value) Base fertilizer: organic fertilizer plus rhamnolipin (100 g / mu), soil improvement plus root promotion.

[0081] Topdressing: Add macronutrients and rhamnolipin (1:80) and drip irrigate once every 10 days.

[0082] Foliar application: Apply a mixture of micronutrients and rhamnolipin (1:30) during the critical period.

[0083] Disease prevention: Regularly administer 50–100 mg / L to prevent fungal diseases.

[0084] Resistance: Spray 20–50 mg / L before adverse conditions occur.

[0085] Results Summary: Fertilizer usage reduced by 10%–20%, yield increased by 10%–15%, quality improved, soil healthier, and input-output ratio high.

[0086] Instructions for use of bio-enhancing stimulants Yizhihe Bio-Enhancing Stimulant CBN-601SF Broad-spectrum antiviral biosurfactant Ingredients: Fermentation-derived potent bioactive substance (surfactin analogue).

[0087] Triple Mechanism 1. When the surface tension drops below 30 mN / m, the wetting and spreading of the medicine solution is excellent.

[0088] 2. Activate the salicylic acid pathway and induce systemic resistance (SAR).

[0089] 3. Physically destroys the cell membrane of pathogenic fungi and the lipid envelope of enveloped viruses, thus preventing the development of drug resistance.

[0090] Efficacy: Prevents powdery mildew, gray mold, and viral diseases (TMV, etc.); as an adjuvant for aerial spraying, it reduces chemical pesticide use by 30%-50%.

[0091] Usage: Dilute 300-500 times for foliar spraying.

[0092] Packaging: 1L / 5L bottles. Store in a cool, dark place.

[0093] Yizhihe Bio-Enhancing Stimulant CBN-602RL Oomycete-specific inhibitor and rhizosphere microecological activator Ingredients: Bioactive glycolipids with specific structures.

[0094] The three main advantages are as follows: 1. Specific inhibitory fungi: Highly effective against Phytophthora and Pythium (soybean blight, potato late blight, cucumber damping-off).

[0095] 2. Rhizosphere carbon source: preferentially utilized by beneficial microorganisms, promoting the proliferation of rhizosphere microbiota.

[0096] 3. Activate immunity: Induce plants to develop broad-spectrum resistance to a variety of fungi.

[0097] Efficacy: It disrupts the zoospore membrane, inhibiting spore germination; it also activates trace elements such as calcium, magnesium, and iron in the soil.

[0098] Usage: Root irrigation or drip irrigation, diluted 500-1000 times, 200-300mL per mu.

[0099] Packaging: 1L / 5L bottles. Store in a cool, dark place.

[0100] Yizhihe Bio-Enhancing Stimulant CBN-603BD Stress regulation expert: drought and cold resistance signal inducer Ingredients: Lipopeptide signaling molecules that mimic beneficial rhizosphere bacteria (micromolar level activity).

[0101] Two major advantages 1. Precise induction of stress resistance pathways: Activation of jasmonic acid / ethylene and abscisic acid pathways, regulation of stomatal opening and closing, and a 30%-40% reduction in water transpiration.

[0102] 2. Systemic stress resistance: One application provides drought / cold resistance for 2-3 weeks. It is non-hormonal and does not interfere with normal growth.

[0103] Efficacy: Maintains leaf water content under drought conditions; improves cell membrane stability and reduces frost damage before low temperatures.

[0104] Usage: Apply as a foliar spray or root spray 1-3 days before the onset of adverse conditions (recommended), diluted 2000-3000 times.

[0105] Packaging: 1L / 5L bottles. Store in a cool, dark place.

[0106] Key operating points: Stir thoroughly after each addition before adding the next. Adding lipopeptides and rhamnolipids last can reduce foaming. After drip irrigation is complete, flush the pipes with clean water for 10-15 minutes.

[0107] The above embodiments are only used to illustrate the present invention and are not intended to limit the technical solutions described herein. Although the present invention has been described in detail with reference to the above embodiments, the present invention is not limited to the specific embodiments described above. Therefore, any modifications or equivalent substitutions to the present invention, as well as all technical solutions and improvements that do not depart from the spirit and scope of the invention, are covered within the scope of the claims of the present invention.

Claims

1. A method for recruiting nitrogen-fixing bacteria using lipopeptides to promote straw decomposition, characterized in that, Includes the following steps: Step 1, straw return to the field: After harvest, crush the crop straw in the field, spread it evenly on the field surface and turn it into the topsoil. Keep the topsoil moist to create a soil microenvironment suitable for the colonization and reproduction of nitrogen-fixing bacteria and straw decomposition microorganisms. Step 2, Selection of drug components: A bio-enhancing stimulant system is selected for application. The bio-enhancing stimulant system includes at least one of CBN-601SF, CBN-602RL, and CBN-603BD, wherein CBN-603BD contains a lipopeptide signaling molecule for targeted recruitment of soil nitrogen-fixing bacteria. Step 3, Precise preparation of the drug solution: Dilute each drug component step by step with clean water, following the principle of preparing and using immediately. Finally, add the active components such as lipopeptides and glycolipids and stir evenly to avoid foaming. Do not mix with strong acidic materials with pH < 4 or strong alkaline materials with pH > 9. Step 4, First targeted application: Within 1 to 3 days after the straw is turned back into the field, apply the prepared pesticide solution evenly into the straw-returned soil layer using drip irrigation or flood irrigation, and use the active components of lipopeptides and glycolipids to quickly wet the interface between the straw and the soil. Step 5, Periodic application and maintenance: Apply 2-3 times in a 7-10 day cycle. The total amount of the biological stimulant system stock solution applied per acre per application is 1-2 kg to stabilize the activity of beneficial bacteria in the soil. Step 6, Pipeline cleaning: After each drip irrigation application, rinse the drip irrigation pipeline with clean water for 10-15 minutes; Step 7, Soil microecological activation: CBN-602RL glycolipid components provide nitrogen-fixing bacteria with a usable rhizosphere carbon source and chelate and activate soil trace elements such as calcium, magnesium, and iron. Step 8: Maintaining the homeostasis of the decomposition environment: Activating plant stress resistance pathways through CBN-603BD lipopeptide signaling molecules, regulating soil and plant water transpiration, maintaining stable soil moisture throughout the straw decomposition process, and continuously ensuring the proliferation of nitrogen-fixing bacteria and the decomposition efficiency of straw organic matter.

2. The method for recruiting nitrogen-fixing bacteria to promote straw decomposition using lipopeptides according to claim 1, characterized in that, The CBN-603BD is an active preparation containing micromolar lipopeptide signaling molecules with an effective ingredient concentration of 50 g / L. The effective working concentration in straw-returned soil is controlled at 20–30 mg / L. It can precisely activate the jasmonic acid / ethylene and abscisic acid pathways, induce systemic stress resistance in plants, and reduce water transpiration by 30%–40%.

3. The method for recruiting nitrogen-fixing bacteria to promote straw decomposition using lipopeptides according to claim 1, characterized in that, The CBN-602RL is a rhizosphere microecological activator containing specific structural glycolipid bioactive substances, with an effective ingredient concentration of 50 g / L and a working concentration of 50–100 mg / L in straw-returned soil.

4. The method for recruiting nitrogen-fixing bacteria to promote straw decomposition using lipopeptides according to claim 3, characterized in that, The CBN-603BD and CBN-602RL are applied in combination, with a final concentration ratio of 1:2 to 1:

5. After the combination, they are applied to the soil using an integrated water and fertilizer drip irrigation method.

5. The method for recruiting nitrogen-fixing bacteria to promote straw decomposition using lipopeptides according to claim 1, characterized in that, The CBN-601SF contains fermentation-derived surfactantin analog lipopeptide active substances with an effective ingredient concentration of 5 g / L. The effective application concentration is 30–50 mg / L, which reduces the surface tension of the pesticide solution at the soil-straw interface to below 30 mN / m.

6. The method for recruiting nitrogen-fixing bacteria to promote straw decomposition using lipopeptides according to claim 1, characterized in that, The bio-enhancing stimulant system can be mixed with water-soluble fertilizers, micronutrient fertilizers, and Bacillus subtilis-based biological pesticides. When mixing, the fertilizer or pesticide should be diluted first, and the bio-enhancing stimulant component should be added last.

7. The method for recruiting nitrogen-fixing bacteria to promote straw decomposition using lipopeptides according to claim 1, characterized in that, Application of pesticides should be carried out in the early morning or late afternoon when there is no strong sunlight or high temperature. The soil should be kept moist during the application process to facilitate the diffusion of active ingredients and the adsorption and colonization of nitrogen-fixing bacteria and decomposing bacteria on the surface of straw.

8. The method for recruiting nitrogen-fixing bacteria to promote straw decomposition using lipopeptides according to claim 3, characterized in that, The CBN-602RL activates soil trace elements such as calcium, magnesium, and iron through chelation, thereby releasing soil nutrient fixation and providing sufficient nutrient substrates for the metabolic proliferation of nitrogen-fixing bacteria and the microbial decomposition of straw organic matter.

9. The method for recruiting nitrogen-fixing bacteria using lipopeptides to promote straw decomposition according to claim 2, characterized in that, The CBN-603BD is a non-hormonal active preparation that does not interfere with plant growth. After a single application, it can maintain the stress resistance of the soil and plants for 2 to 3 weeks and stabilize the microenvironment for straw decomposition.

10. The method for recruiting nitrogen-fixing bacteria to promote straw decomposition using lipopeptides according to claim 1, characterized in that, The entire method utilizes multiple synergistic effects of lipopeptide signaling molecules to recruit nitrogen-fixing bacteria for colonization, glycolipid carbon supplementation to promote bacterial growth, activation of soil nutrients, and stabilization of the soil microenvironment.

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