Preparation method and application of andrias davidianus glue

Abstract

The application relates to a preparation method and application of andrias davidianus glue. The andrias davidianus skin of artificial breeding is used as raw material, low-temperature decoction extraction and gradient condensation drying are carried out, the heat-sensitive active ingredients in the andrias davidianus skin are maximally reserved, the andrias davidianus glue extracted retains more active ingredients such as metallothionein, glycopeptide and ranaplatin, and can be used for preparing health-care food for resisting fatigue and improving immunity.

Description

Technical Field

[0001] This invention belongs to the field of animal glue preparation technology, and in particular relates to a method for preparing and applying giant salamander glue. Background Technology

[0002] The giant salamander (Andrias davidianus), also known as the Chinese giant salamander, is a rare amphibian with rich biological characteristics and medicinal value. Its medicinal value is mainly reflected in its various bioactive components. Its skin, muscles, internal organs, bones, epidermis, and secretions can all be used medicinally, possessing effects such as replenishing qi, nourishing blood, improving intelligence, tonifying, and strengthening the body. It is mainly used to treat neurasthenia, anemia, dysentery, malaria, and other ailments, and is also used for post-illness and postpartum weakness, kidney deficiency, pulmonary tuberculosis with hemoptysis, and chronic dysentery with rectal prolapse. Modern medical research shows that the giant salamander is rich in various functional components, such as metallothionein (MT), frog skin protein, active collagen, and epidermal whitening factor, which have antioxidant, antibacterial, antitumor, anti-aging, and immune-enhancing effects. Furthermore, the giant salamander's muscles are rich in ω-3 and ω-6 polyunsaturated fatty acids, which can prevent cardiovascular and cerebrovascular diseases, regulate heart function, improve blood circulation, and enhance memory and thinking ability. The mucus of the giant salamander contains "frog dermatin," which has strong antibacterial properties and can be studied and developed as a new source of antibiotics. The skin of the giant salamander also contains abundant bioactive peptides (BPs), which possess a wide range of biological activities, including antioxidant, antibacterial, antitumor, and antidiabetic properties. These bioactive peptides have potential applications in the food and biomedical fields, particularly in multifunctional food additives, dietary supplements, and drug delivery systems. The giant salamander is not only an important ecological resource but also has extremely high edible and medicinal value, earning it the titles of "ginseng of the water" and "ancient regenerative factor." Current processing of giant salamanders is mainly for consumption, with the skin mostly discarded as waste, resulting in low resource utilization and a lack of standardized colloid preparation processes. Summary of the Invention

[0003] To solve the above-mentioned technical problems, the present invention provides a method for preparing giant salamander glue and its application.

[0004] The technical solution adopted in this invention is: a method for preparing giant salamander glue, comprising the following steps: Step 1: Remove the fishy smell and color from the giant salamander skin, scrape off the subcutaneous pigment cells and residual muscle to obtain a milky white, semi-transparent skin. Step 2: Slice the skin material into sections and boil it. First, add water at a mass ratio of 1:3-10 and boil it for the first time, then discard the liquid. Next, add water at a mass ratio of 1:3-10 and extract at a constant temperature of 70-80℃ for 4-8 hours. Filter and collect the filtrate. Add water to the skin material again and boil it for extraction 1-3 times. Combine the filtrates and concentrate them under reduced pressure to obtain the initial product of giant salamander glue. Step 3: Inject the initial product of giant salamander glue into the mold, pre-cool it at 5℃ for 1.5-2 hours to set the shape; then place the giant salamander glue in -20℃ for quick freezing for 3-5 hours to condense the initial product of giant salamander glue; after condensation, place the giant salamander glue in an environment of 20-25℃ to dry.

[0005] Preferably, in step two, 0.05-0.2% alum powder is added to the combined filtrate, stirred evenly, and allowed to stand for 3-4 hours to remove precipitated impurities. Then, the filtrate is filtered through a 10 kDa ultrafiltration membrane to remove small molecule impurities.

[0006] Preferably, the filtrate is concentrated under reduced pressure at 60°C until the solid content is ≥35%, and the resulting product is the initial product of giant salamander glue.

[0007] Preferably, in step three, the condensed giant salamander glue is placed in a constant humidity environment of 25°C and RH 40-50% to dry the glue until the moisture content is ≤12%.

[0008] Preferably, in step one, fresh giant salamander skin is immersed in an aqueous solution containing 0.3-0.7% citric acid and 1-3% rice wine to deodorize and decolorize the skin, and then soaked in an environment of 4°C for 48-72 hours, changing the solution every 12 hours.

[0009] Preferably, the dried giant salamander glue is sterilized by ultraviolet light, and then the surface is coated with collagen.

[0010] Preferably, the initial product of giant salamander glue is flavored and then condensed; 3-5% (w / w) mucus hydrolysate, 1-3% rock sugar and 1-2% sorbitol are added to the initial product of giant salamander glue.

[0011] A type of giant salamander glue, prepared by a method for preparing giant salamander glue.

[0012] Preferably, the metallothionein content is not less than 50 μg / g, and the glycopeptide content is not less than 8%.

[0013] Application of giant salamander glue in health food.

[0014] The advantages and positive effects of this invention are: it provides a method for preparing giant salamander gel, which maximizes the preservation of the functions of active substances in giant salamander skin extract through low-temperature extraction and gradient condensation processes. The resulting gel product has better strength and can be used in products that combat fatigue and enhance immunity. Attached Figure Description

[0015] Figure 1 Analysis of the effect of giant salamander glue on swimming time in mice. Detailed Implementation

[0016] The embodiments of the present invention will now be described with reference to the accompanying drawings.

[0017] This invention relates to a method for preparing and applying giant salamander gelatin. Using the skin of artificially bred giant salamanders as raw material, the method involves low-temperature decoction extraction and gradient condensation drying to maximize the retention of heat-sensitive active ingredients in the giant salamander skin. The extracted giant salamander gelatin retains a large amount of active ingredients such as metallothionein, glycopeptides, and dinoflagellins, and can be used to prepare health foods that combat fatigue and enhance immunity.

[0018] The specific extraction method includes the following steps: Step 1: Pre-treatment of Giant Salamander Skin. Obtain skin from artificially farmed giant salamanders (second generation or above), ensuring legal sourcing and compliance with GB2762 for heavy metal residues. Immerse the fresh salamander skin in an aqueous solution containing 0.3-0.7% citric acid and 1-3% rice wine to deodorize and decolorize the skin. Soak at 4°C for 48-72 hours, changing the solution every 12 hours. Mechanically scrape away subcutaneous pigment cells and residual muscle to obtain a milky white, semi-transparent skin.

[0019] Step Two: Extraction of Giant Salamander Glue. A segmented decoction method is used to extract giant salamander glue from the salamander skin. The skin is cut into pieces. For the first decoction, water is added at a mass ratio of 1:3-10. After the first boil, the liquid portion is discarded to remove impurities. For the second decoction, water is again added at a mass ratio of 1:3-10, and extraction is carried out at a constant temperature of 70-80℃ for 4-8 hours. This decoction process is repeated 1-3 times.

[0020] The filtrates obtained from multiple decoctions were combined and then clarified. 0.05-0.2% alum powder was added to the coarse filtrate, stirred evenly, and allowed to stand for 3-4 hours to remove precipitated impurities. The liquid portion was then filtered through a 10 kDa ultrafiltration membrane to remove small molecule impurities and retain functional peptides. The filtered product was then concentrated under reduced pressure at 60°C until the solid content was ≥35%. The resulting product is the primary product of giant salamander glue.

[0021] Step 3: Pour the prepared giant salamander glue initial product into a mold, pre-cool at 5℃ for 1.5-2 hours to set the shape; then place the mold containing the giant salamander glue in a -20℃ quick-freeze environment for 3-5 hours, further improving the Bloom strength to >200g through gradient condensation. Place the condensed giant salamander glue in a 25℃ constant humidity (RH 40-50%) environment and air-dry for 72 hours; then dry with far-infrared radiation until the moisture content is ≤12%. After the dried giant salamander glue is sterilized by ultraviolet light, the surface is wiped with collagen liquid to form a protective film, which protects the stability of the surface components of the giant salamander glue.

[0022] The above preparation method, employing low-temperature extraction and gradient condensation, maximizes the preservation of the active ingredients in the giant salamander glue. Furthermore, the combination of segmented decoction and alum treatment addresses the issue of turbidity in the glue solution, ensuring the quality of the prepared giant salamander glue. In some embodiments of this invention, to improve the odor of the giant salamander glue, the primary product can be flavored by adding mucin hydrolysate (3-5% w / w), rock sugar (1-3%), and sorbitol (1-2%), which improves the odor while enhancing its antioxidant properties.

[0023] Testing of the prepared giant salamander glue revealed that the gel strength of the glue prepared by the above method was greater than or equal to 220 g (Bloom method), and its active ingredient, metallothionein, was not less than 50 μg / g, glycopeptides were not less than 8%, and the heavy metal lead content was ≤0.3 ppm (stricter than EU standards). Research showed that metallothionein, glycopeptides, and collagen peptides are often the main active ingredients in anti-fatigue products. Giant salamander glue extracted under specific temperature conditions using this method is particularly suitable for preparing anti-fatigue products, such as those for combating fatigue and boosting immunity.

[0024] The present invention will now be described with reference to the accompanying drawings. Experimental methods not specifically described in terms of operation steps are performed in accordance with the corresponding product manuals. Unless otherwise specified, the instruments, reagents, and consumables used in the embodiments can be purchased from commercial companies. Example 1: Preparation of Giant Salamander Glue

[0025] Skin from artificially bred giant salamanders (second generation or later) is obtained, ensuring legal sourcing and compliance with GB2762 standards for heavy metal residues. The salamander skin is deodorized and decolorized by immersing fresh skin in a mixture of (0.3-0.7%) citric acid and (1-3%) rice wine at 4°C for 48 hours, changing the solution every 12 hours. Subcutaneous pigment cells and residual muscle are mechanically scraped off to obtain a milky white, semi-transparent skin.

[0026] The giant salamander skin was decocted in stages. First decoction: The skin was cut into pieces, and water was added at a mass ratio of 1:3-10. After the first boil, the liquid was discarded, and impurities were removed. Second decoction: Water was added again at a mass ratio of 1:3-10, and the mixture was extracted at a constant temperature of 70-80℃ for 4-8 hours. This process was repeated 3 times, and the filtrates were combined.

[0027] The filtrate was clarified by multiple steps. 0.05-0.2% alum powder was added to the coarse filtrate and stirred. The mixture was allowed to stand for 4 hours to remove precipitated impurities. The liquid portion was then filtered through a 10 kDa ultrafiltration membrane to remove small molecule impurities while retaining functional peptides. The filtered product was concentrated under reduced pressure at 60°C until the solid content was ≥35%. The resulting product was the giant salamander gelatin.

[0028] The flavor of giant salamander glue can be further improved by adding mucin hydrolysate (5% w / w), rock sugar (3%), and sorbitol (2%) to improve the odor and enhance its antioxidant properties.

[0029] The prepared giant salamander glue solution was injected into a mold and pre-cooled at 5°C for 2 hours to set. The mold containing the giant salamander glue was then placed at -20°C for 4 hours to further improve the Bloom strength to >200 g.

[0030] The giant salamander glue was then placed in a constant humidity environment (RH 40-50%) at 25℃ and allowed to dry for 72 hours; it was then dried with far-infrared until the moisture content was ≤12%. After UV sterilization, the surface was wiped with collagen solution to form a protective film. The prepared giant salamander glue was tested and found to have a gel strength greater than or equal to 220 g (Bloom method), an active ingredient metallothionein of 67 μg / g (not less than 50 μg / g), glycopeptides of 9.5% (not less than 8%), collagen peptides of 1,000-10,000 Da (this range is easily absorbed by the body) distribution ratio of 55.6%, and heavy metal lead ≤0.3 ppm (stricter than EU standards). Comparative Example 1:

[0031] Skin from artificially bred giant salamanders (second generation or later) is obtained, ensuring legal sourcing and compliance with GB2762 standards for heavy metal residues. The salamander skin is deodorized and decolorized by immersing fresh skin in a mixture of (0.3-0.7%) citric acid and (1-3%) rice wine at 4°C for 48 hours, changing the solution every 12 hours. Subcutaneous pigment cells and residual muscle are mechanically scraped off to obtain a milky white, semi-transparent skin.

[0032] Water extraction of giant salamander skin is performed. Water is added at a mass ratio of 1:3-10, and after the first boil, the liquid portion is discarded, and impurities are removed. Second decoction: Water is added again at a mass ratio of 1:3-10, and the mixture is boiled for 2-3 hours. This process is repeated 3 times, and the filtrates are combined.

[0033] The filtrate was clarified by multiple steps. 0.05-0.2% alum powder was added to the coarse filtrate and stirred. The mixture was allowed to stand for 4 hours to remove precipitated impurities. The liquid portion was then filtered through a 10 kDa ultrafiltration membrane to remove small molecule impurities while retaining functional peptides. The filtered product was concentrated under reduced pressure at 60°C until the solid content was ≥35%. The resulting product was the giant salamander gelatin.

[0034] To further improve the flavor of giant salamander glue, mucin hydrolysate (5% w / w), rock sugar (3%), and sorbitol (2%) were added to improve the odor and enhance its antioxidant properties.

[0035] The prepared giant salamander glue solution was injected into a mold and pre-cooled at 5°C for 2 hours to set. The mold containing the giant salamander glue was then placed at -20°C for 4 hours to further improve the Bloom strength to >200 g.

[0036] The giant salamander gelatin was then placed in a constant humidity environment (RH 40-50%) at 25℃ and allowed to dry for 72 hours; it was then dried using far-infrared drying until the moisture content was ≤12%. After UV sterilization, the surface was wiped with collagen solution to form a protective film. The prepared giant salamander gelatin was tested, and its gel strength was greater than or equal to 220 g (Bloom method). Its active ingredient, metallothionein, was 3750 μg / g (less than 50 μg / g), and its glycopeptide content was 7.3% (less than 8%), which was significantly lower than that extracted at low temperature in Example 1. Comparative Example 2:

[0037] Skin from artificially bred giant salamanders (second generation or later) is obtained, ensuring legal sourcing and compliance with GB2762 standards for heavy metal residues. The salamander skin is deodorized and decolorized by immersing fresh skin in a mixture of (0.3-0.7%) citric acid and (1-3%) rice wine at 4°C for 48 hours, changing the solution every 12 hours. Subcutaneous pigment cells and residual muscle are mechanically scraped off to obtain a milky white, semi-transparent skin.

[0038] Water extraction was performed on the giant salamander skin. Water was added at a mass ratio of 1:3-10, and after the first boil, the liquid portion was discarded, and impurities were removed. Second decoction: Water was added again at a mass ratio of 1:3-10, and the mixture was extracted at 50-60℃ for 5-8 hours. This process was repeated 3 times, and the filtrates were combined.

[0039] The filtrate was clarified by multiple steps. 0.05-0.2% alum powder was added to the coarse filtrate and stirred. The mixture was allowed to stand for 4 hours to remove precipitated impurities. The liquid portion was then filtered through a 10 kDa ultrafiltration membrane to remove small molecule impurities while retaining functional peptides. The filtered product was concentrated under reduced pressure at 60°C until the solid content was ≥35%. The resulting product was the giant salamander gelatin.

[0040] To further improve the flavor of giant salamander glue, mucin hydrolysate (5% w / w), rock sugar (3%), and sorbitol (2%) were added to improve the odor and enhance its antioxidant properties.

[0041] The prepared giant salamander glue solution was injected into a mold and pre-cooled at 5°C for 2 hours to set. The mold containing the giant salamander glue was then placed at -20°C for 4 hours to further improve the Bloom strength to >200 g.

[0042] The giant salamander gelatin was then placed in a constant humidity environment (RH 40-50%) at 25℃ and allowed to dry for 72 hours; it was then dried using far-infrared drying until the moisture content was ≤12%. After UV sterilization, the surface was wiped with collagen solution to form a protective film. The prepared giant salamander gelatin was tested, and its gel strength was greater than or equal to 220 g (Bloom method). Its active ingredient, metallothionein, was 42 μg / g (less than 50 μg / g), and its glycopeptide content was 7.0% (less than 8%), which was significantly lower than that extracted at 70-80℃ in Example 1. Comparative Example 3: Preparation of Giant Salamander Glue by Enzymatic Hydrolysis

[0043] Skin from artificially bred giant salamanders (second generation or later) is obtained, ensuring legal sourcing and compliance with GB2762 standards for heavy metal residues. The salamander skin is deodorized and decolorized by immersing fresh skin in a mixture of (0.3-0.7%) citric acid and (1-3%) rice wine at 4°C for 48 hours, changing the solution every 12 hours. Subcutaneous pigment cells and residual muscle are mechanically scraped off to obtain a milky white, semi-transparent skin.

[0044] Water extraction was performed on the giant salamander skin. Water was added at a mass ratio of 1:3-10, and neutral protease (NP) (6000-8000 U / g) was added. The mixture was enzymatically hydrolyzed at 50-60℃ for 4-5 hours. After the enzymatic hydrolysis was completed, the enzyme was immediately inactivated by heating at 90℃ for 10 minutes, and the filtrate was obtained by filtration.

[0045] The filtrate was clarified by multiple steps. 0.05-0.2% alum powder was added to the coarse filtrate and stirred. The mixture was allowed to stand for 4 hours to remove precipitated impurities. The liquid portion was then filtered through a 10 kDa ultrafiltration membrane to remove small molecule impurities while retaining functional peptides. The filtered product was concentrated under reduced pressure at 60°C until the solid content was ≥35%. The resulting product was the giant salamander gelatin.

[0046] To further improve the flavor of giant salamander glue, mucin hydrolysate (5% w / w), rock sugar (3%), and sorbitol (2%) were added to improve the odor and enhance its antioxidant properties.

[0047] The prepared giant salamander glue solution was injected into a mold and pre-cooled at 5°C for 2 hours to set. The mold containing the giant salamander glue was then placed at -20°C for 4 hours to further improve the Bloom strength to >200 g.

[0048] Next, place the giant salamander glue in a constant humidity environment at 25℃ (RH 40-50%) and let it air dry for 72 hours; then dry it with far-infrared until the moisture content is ≤12%. After ultraviolet sterilization, wipe the surface with collagen liquid to form a protective film.

[0049] The prepared giant salamander gel was tested, and its gel strength was greater than or equal to 220 g (Bloom method). Its active ingredient metallothionein was 53 μg / g (not less than 50 μg / g), glycopeptides were 8.3% (not less than 8%), and collagen peptides of 1,000-10,000 Da (this range is easily absorbed by the body) accounted for 43.7%, which is relatively less than that in Example 1. Example 2: Study on the fatigue-relieving effect of giant salamander glue

[0050] Forty-eight healthy KM mice, weighing 20±2g, half male and half female, were selected and divided into a blank control group, a positive control group, and a group with different doses of giant salamanders. Each group consisted of six mice, three males and three females. The mice were raised separately, weighed, and their weight was recorded.

[0051] The positive control group used isolated soy protein powder (ISPP). Analysis of the composition of protein-based health foods that can alleviate physiological fatigue and enhance immunity shows that isolated soy protein powder is often a major ingredient in these products, playing a significant role in their efficacy. The efficacy of ISPP was compared with that of giant salamander gelatin. The recommended daily intake for humans is generally 5g. Based on 5, 10, and 20 times the recommended daily intake, the giant salamander group was divided into three dosage groups: 0.5g / kg·d, 1g / kg·d, and 2g / kg·d. The three giant salamander gelatin dosage groups were administered the prescribed doses. The positive control group received 3g / kg·d of ISPP via gavage, and the control group received 0.2ml / 20g of ISPP based on mouse body weight via gavage. The blank control group received physiological saline via gavage. This was done once daily for 30 consecutive days.

[0052] Thirty minutes after the last gavage, the mice were quickly lifted by their tails and placed in a pool of water (at least 30 cm deep and approximately 25°C) to swim. The swimming performance of the mice in each experimental group was observed, and the time from the start of swimming to death was accurately recorded as the swimming time. Specific results are shown in Table 1 and... Figure 1 As shown.

[0053] From Table 1 and Figure 1 It was found that the swimming time of mice in the ISPP group and the three giant salamander glue groups was longer than that of the control group, proving that both ISPP and giant salamander glue can improve the endurance of mice. The ISPP group and the three giant salamander glue groups with different doses showed different degrees of improvement compared with the control group. Among them, the swimming time of mice in the 2 g / kg·d group was significantly prolonged (P<0.01), proving that this dose has a prominent effect on improving the endurance of mice.

[0054] Table 1. Analysis of the effect of giant salamander glue on swimming time in mice (x±s, n=6) Example 3: Study on the efficacy of giant salamander gelatin in improving low immunity

[0055] Twenty-four healthy KM mice, weighing 20±2g, half male and half female, were divided into four groups. Each group consisted of a blank control group, a positive control group, and different doses of giant salamander gelatin groups, with six mice in each dose group (three males and three females). The mice were housed separately and their weights were recorded. The positive control group used soy protein isolate (ISPP). The giant salamander groups were divided into three dose groups: 0.5g / kg·d, 1g / kg·d, and 2g / kg·d PPPSM.

[0056] Three dosage groups of giant salamander gelatin were administered according to the set dosage. The positive control group was given 3 g / kg·d of ISPP by gavage, and the negative control group was given 0.2 ml / 20 g of salamander gelatin by gavage. The administration was once daily for 30 consecutive days. After 30 days, the body weight, spleen index, thymus index, and liver index of the mice were analyzed and compared. The results are shown in Figure 2.

[0057] Table 2. Analysis of the effects of PPPSM on mouse body weight and organ index (x±s, n=6)

[0058] *p<0.05 vs. blank group Based on the data in Table 2, the body weight of mice in each experimental group was compared, and the spleen index was also compared. Compared with the control group, the spleen index of the 2 g / kg·d giant salamander gelatin group was significantly increased (P<0.05), indicating that 2 g / kg·d giant salamander gelatin can significantly increase the spleen index of mice. Comparing the thymus index, compared with the control group, the thymus index of the 0.5 g / kg·d, 1 g / kg·d, and 2 g / kg·d giant salamander gelatin groups were all significantly increased (P<0.05), indicating that 0.5 g / kg·d, 1 g / kg·d, and 2 g / kg·d giant salamander gelatin can all significantly increase the thymus index. Comparing the liver index, compared with the control group, the liver index of both the 2 g / kg·d giant salamander gelatin group and the ISPP group showed significant differences (P<0.05), indicating that 2 g / kg·d giant salamander gelatin and the ISPP group have a significant effect on the liver index of mice. Example 4: A solid beverage containing giant salamander gelatin

[0059] This solid beverage contains giant salamander gelatin, comprising 10-20 parts giant salamander gelatin, 10-20 parts Ganoderma lucidum polysaccharide, 10-20 parts white sugar, 20-30 parts maltodextrin, and 1-2 parts citric acid. Each 5g packet is packaged. One packet is recommended daily for up to three consecutive months. Example 5: An oral tablet containing giant salamander gelatin

[0060] An oral tablet comprises 5-10 parts of giant salamander gelatin powder, 2-5 parts of microcrystalline cellulose, 25-45 parts of maltodextrin, 2-5 parts of HPMC, and 2-5 parts of (magnesium stearate and sodium lauryl sulfate). The raw materials are mixed and then wet-granulated to obtain an oral tablet with a tablet weight of 500 mg and a disintegration time of <10 min. Oral administration: 1 tablet (500 mg / tablet) daily for 3 consecutive months. Example 6: Preparation of a capsule containing giant salamander gelatin

[0061] Capsules containing giant salamander gelatin consist of giant salamander gelatin powder mixed with excipients (maltodextrin) at a mass ratio of 1:10-20, filled into capsules, each capsule containing 200mg. Oral dosage: 2 capsules daily for 3 consecutive months.

[0062] The embodiments of the present invention have been described in detail above, but the content described is only a preferred embodiment of the present invention and should not be considered as limiting the scope of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the patent coverage of the present invention.

Claims

1. A method for preparing giant salamander glue, characterized in that: Includes the following steps: Step 1: Remove the fishy smell and color from the giant salamander skin, scrape off the subcutaneous pigment cells and residual muscle to obtain a milky white, semi-transparent skin. Step 2: Slice the skin material into sections and boil it. First, add water at a mass ratio of 1:3-10 and boil it for the first time, then discard the liquid. Next, add water at a mass ratio of 1:3-10 and extract at a constant temperature of 70-80℃ for 4-8 hours. Filter and collect the filtrate. Add water to the skin material again and boil it for extraction 1-3 times. Combine the filtrates and concentrate them under reduced pressure to obtain the initial product of giant salamander glue. Step 3: Inject the initial product of giant salamander glue into the mold, pre-cool it at 5℃ for 1.5-2 hours to set the shape; then place the giant salamander glue in -20℃ for quick freezing for 3-5 hours to condense the initial product of giant salamander glue; after condensation, place the giant salamander glue in an environment of 20-25℃ to dry.

2. The method for preparing giant salamander glue according to claim 1, characterized in that: In step two, add 0.05-0.2% alum powder to the combined filtrate, stir well, let stand for 3-4 hours to remove precipitated impurities, and then pass through a 10 kDa ultrafiltration membrane to remove small molecule impurities.

3. The method for preparing giant salamander glue according to claim 2, characterized in that: The filtrate was concentrated under reduced pressure at 60°C until the solid content was ≥35%, and the resulting product was the initial product of giant salamander glue.

4. The method for preparing giant salamander glue according to claim 1, characterized in that: In step three, the condensed giant salamander glue is placed in a constant humidity environment of 25°C and RH 40-50% to dry the glue until the moisture content is ≤12%.

5. The method for preparing giant salamander glue according to claim 1, characterized in that: Step 1: Soak fresh giant salamander skin in an aqueous solution containing 0.3-0.7% citric acid and 1-3% rice wine to remove the fishy smell and color from the skin. Soak the skin at 4°C for 48-72 hours, changing the solution every 12 hours.

6. The method for preparing giant salamander glue according to any one of claims 1-5, characterized in that: The dried giant salamander glue is sterilized with ultraviolet light, and then the surface is coated with collagen.

7. The method for preparing giant salamander glue according to claim 6, characterized in that: Flavor the initial product of giant salamander glue and then condense it; add 3-5% (w / w) mucus hydrolysate, 1-3% rock sugar and 1-2% sorbitol to the initial product of giant salamander glue.

8. A type of giant salamander glue, characterized in that: It is prepared by the method for preparing giant salamander glue according to any one of claims 1-7.

9. The giant salamander glue according to claim 8, characterized in that: Metallothionein is not less than 50 μg / g, and glycopeptides are not less than 8%.

10. The application of the giant salamander glue according to claim 8 or 9 in health food.

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