Syncom y and application thereof in promoting plant growth

Abstract

The application belongs to the technical field of microorganisms and particularly relates to a growth promoting microbial inoculant SynComY and application thereof in promoting plant growth. Acinetobacter lwoffii X1, Pseudomonas sp. Pseudomonas hunanensis L5 and Pseudomonas ceruminis Y39, which are preserved in the China Center for Type Culture Collection on June 10, 2026, and have the preservation numbers CCTCC M 20261263, CCTCC M 20261264 and CCTCC M 20261268, respectively. The SynComY has the abilities of producing siderophores, producing biofilms, dissolving calcium phosphate, dissolving insoluble inorganic phosphorus and insoluble organic phosphorus such as lecithin, and has obvious promoting effect on the growth of alfalfa and improves the biomass of alfalfa.

Description

Technical Field

[0001] This invention belongs to the field of microbial growth promotion technology, specifically relating to a growth-promoting agent SynComY and its application in promoting plant growth. Background Technology

[0002] As modern agriculture develops towards green, efficient, and sustainable directions, utilizing beneficial microorganisms to promote crop growth has become an important research direction in agricultural biotechnology. Soil and plant-related microorganisms are widely involved in processes such as nutrient transformation, plant hormone regulation, and rhizosphere ecosystem construction, and have a significant impact on plant growth and development. Among them, plant growth-promoting bacteria can promote the absorption and utilization of nutrients by plants through mechanisms such as nitrogen fixation, phosphorus solubilization, and auxin production, thereby improving crop growth performance. Therefore, they are widely used in the development of microbial inoculants.

[0003] Alfalfa is a globally important legume forage and feed crop, characterized by high yield, rich nutritional value, and strong nitrogen-fixing capacity, playing a vital role in livestock production. To meet the demands of high-yield and high-quality alfalfa production, traditional agricultural production often relies on chemical fertilizers. However, long-term application of chemical fertilizers not only increases production costs but may also adversely affect the soil and ecological environment. Therefore, developing safe, environmentally friendly, and efficient microbial inoculants to replace some chemical inputs has become an important direction for the green production of alfalfa.

[0004] In recent years, research has revealed that plant endophytic bacteria, as beneficial microorganisms that colonize plant tissues long-term, form stable symbiotic relationships with their host plants. Compared to exogenous microorganisms, endophytic bacteria typically possess stronger host adaptability and colonization capabilities, demonstrating significant potential for promoting plant growth. In particular, endophytic bacteria resources derived from alfalfa itself hold promise for playing a sustained and stable role in alfalfa production.

[0005] Most existing microbial inoculants are based on single strains, but these often suffer from limitations in practical applications, such as limited functionality, environmental adaptability, and unstable growth-promoting effects. With the development of plant microbiome research, it has become increasingly clear that plant growth is influenced by the synergistic effects of multiple microorganisms. Synthetic communities (SynCom) constructed based on different functional strains can fully leverage the complementary and synergistic effects among strains, demonstrating superior application potential compared to single-strain in promoting plant growth.

[0006] However, there is still a lack of compound microbial agents for promoting alfalfa growth, especially synthetic microbial products based on alfalfa-derived endophytic bacteria that have a stable growth-promoting effect. Therefore, developing a compound microbial growth-promoting agent composed of beneficial alfalfa endophytic bacteria and applying it to promote alfalfa growth and increase biomass production is of great significance for promoting green and efficient alfalfa production and the development and utilization of microbial resources. Summary of the Invention

[0007] In order to solve the problems existing in the prior art, one of the objectives of the present invention is to provide a compound microbial inoculant for alfalfa.

[0008] The primary objective of this invention is to provide a *Acinetobacter rumenella* (… Acinetobacter lwoffii X1 was deposited at the China Center for Type Culture Collection on June 10, 2026, with accession number CCTCC M 20261263.

[0009] The second objective of this invention is to provide a *Pseudomonas hunanense* ( Pseudomonas hunanensis L5 was deposited at the China Center for Type Culture Collection on June 10, 2026, with accession number CCTCC M 20261264.

[0010] The third objective of this invention is to provide a Pseudomonas ceruminum Y39 was deposited at the China Center for Type Culture Collection on June 10, 2026, with accession number CCTCC M 20261268.

[0011] The fourth objective of this invention is to provide a growth-promoting agent, SynComY, which includes Acinetobacter rouxii (…). Acinetobacter lwoffii X1, Hunan Pseudomonas ( Pseudomonas hunanensis L5 and Pseudomonas ceruminum Y39 was deposited at the China Center for Type Culture Collection on June 10, 2026, with accession numbers CCTCC M 20261263, CCTCC M 20261264, and CCTCC M 20261268, respectively.

[0012] Preferably, the growth-promoting agent SynComY is composed of Acinetobacter rumeni (… Acinetobacter lwoffii X1, Hunan Pseudomonas ( Pseudomonas hunanensis L5 and Pseudomonas ceruminum It consists of Y39, in a ratio of 1:1:1.

[0013] Preferably, the growth-promoting bacterial agent SynComR is a liquid bacterial agent.

[0014] Preferably, the liquid bacterial agent contains ≥1×10⁶ live bacteria. 8 CFU·mL-1 .

[0015] The fifth objective of this invention is to provide the aforementioned Acinetobacter rumeni ( Acinetobacter lwoffii X1, the aforementioned Hunan Pseudomonas ( Pseudomonas hunanensis L5, or the aforementioned Pseudomonas ceruminum Application of Y39 or the aforementioned growth-promoting bacterial agent SynComY in the preparation of plant growth-promoting agents.

[0016] Preferably, the plant is a legume.

[0017] The sixth objective of this invention is to provide the aforementioned Acinetobacter rumenella X1, the aforementioned Pseudomonas hunanense L5, or the aforementioned Pseudomonas ceruminum Application of Y39 or the aforementioned growth-promoting bacterial agent SynComY in the preparation of materials for increasing plant biomass.

[0018] The seventh objective of this invention is to provide the aforementioned Acinetobacter rumenella X1, the aforementioned Pseudomonas hunanense L5, or the aforementioned Pseudomonas ceruminum Application of Y39 or the aforementioned growth-promoting bacterial agent SynComY in formulations that improve the absorption of phosphorus / iron by plant roots.

[0019] The eighth objective of this invention is to provide the aforementioned *Pseudomonas hunanensis* L5, or the aforementioned... Pseudomonas cerumen Application of Y39 or the aforementioned growth promoter SynComY in the remediation of soil / water bodies contaminated with organophosphorus pesticides.

[0020] The ninth objective of this invention is to provide the aforementioned *Pseudomonas aeruginosa* L5, or the aforementioned... Pseudomonas cerumen Application of Y39 or the aforementioned growth promoter SynComY in the preparation of biofertilizers with phosphorus solubilizing and organophosphorus pesticide degradation functions.

[0021] By adopting the above technical solution, the present invention has the following beneficial effects: (1) This invention provides a single-strain Acinetobacter ruthenii with growth-promoting effects ( Acinetobacter lwoffii X1, Hunan Pseudomonas ( Pseudomonas hunanensis L5 and Pseudomonas ceruminum Y39 was deposited at the China Center for Type Culture Collection on June 10, 2026, with accession numbers CCTCC M 20261263, CCTCC M 20261264, and CCTCC M 20261268, respectively. A compound inoculum composed of the above-mentioned single strain was also provided, which has a significant promoting effect on plant growth, can significantly increase the accumulation of dry matter in alfalfa, and can increase the height of alfalfa plants.

[0022] (2) The composite microbial agent SynComY constructed in this invention consists of Acinetobacter rumeniella X1, Pseudomonas hunanense L5, and Pseudomonas ceruminum Composed of Y39, all three strains possess plant growth-promoting functions, including the ability to produce siderophores and activate phosphorus. Furthermore, the strains in SynComY also have the ability to dissolve or mineralize soil phosphorus, converting inorganic and organic phosphorus in the soil that are difficult for plants to directly utilize into absorbable and usable phosphorus, thus improving phosphorus utilization efficiency. Specifically, inorganic phosphorus activation mainly promotes the dissolution of insoluble phosphates through the secretion of metabolic products such as organic acids; organic phosphorus activation mainly relies on the secretion of extracellular enzymes such as phosphatases to promote the mineralization and release of organophosphorus compounds. These functions work together to enhance alfalfa's ability to acquire key nutrients, promoting plant growth and biomass accumulation. Therefore, SynComY of this invention can synergistically exert its growth-promoting effects through multiple mechanisms such as plant hormone regulation, iron activation, and phosphorus activation, providing continuous nutritional support for alfalfa growth. It can also be used to prepare biofertilizers with phosphorus-solubilizing and organophosphorus pesticide degradation functions, demonstrating significant agricultural application value. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other embodiments can be obtained based on these drawings without creative effort.

[0024] Figure 1 Colony morphology Figure 2 Phylogenetic tree of bacterial strains Figure 3 Interstrain antagonism test Figure 4 Organophosphate solubilization ability of strains Figure 5 Strain's ability to solubilize inorganic phosphorus Figure 6 The ability of strains to produce iron carriers Figure 7 The effect of SynComY compound microbial agent on promoting alfalfa growth Detailed Implementation

[0025] Embodiments of this embodiment will now be described in more detail with reference to the accompanying drawings. While some embodiments of this embodiment are shown in the drawings, it should be understood that this embodiment can be implemented in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of this embodiment. It should be understood that the accompanying drawings and embodiments are for illustrative purposes only and are not intended to limit the scope of protection of this embodiment.

[0026] It should be noted that the methods described in the following embodiments are all conventional methods and can be obtained from the literature; the materials and consumables mentioned can be obtained through commercial channels.

[0027] In this invention, the culture medium formulation used is as follows: The LB medium formula is as follows: 10g peptone, 5g yeast extract, 10g NaCl, 15g agar, and 1000mL distilled water.

[0028] The TSB culture medium formula is as follows: 17.0 g tryptone, 3.0 g soybean peptone, 5.0 g sodium chloride, 2.5 g dipotassium hydrogen phosphate, 2.5 g glucose, 15 g agar, and 1000 mL distilled water.

[0029] The R2A culture medium formula is as follows: 0.5 g yeast extract, 0.5 g peptone, 0.5 g casein hydrolysate, 0.5 g glucose, 0.5 g soluble starch, 0.3 g potassium dihydrogen phosphate, 0.024 g anhydrous magnesium sulfate, 0.3 g sodium pyruvate, 15 g agar, and 1000 mL distilled water.

[0030] The cellulose Congo red culture medium formula is as follows: 1 g sodium nitrate, 1.2 g disodium hydrogen phosphate, 0.9 g potassium dihydrogen phosphate, 0.5 g potassium chloride, 0.5 g magnesium sulfate heptahydrate, 0.5 g yeast extract, 0.5 g casein acid hydrolysate, 5 g microcrystalline cellulose, 0.2 g Congo red, 15 g agar, and 1000 mL distilled water.

[0031] The formula for the Monkina culture medium is as follows: 10 g glucose, 0.5 g ammonium sulfate, 0.3 g sodium chloride, 0.3 g magnesium sulfate heptahydrate, 0.03 g manganese sulfate, 0.3 g potassium chloride, 0.03 g ferrous sulfate heptahydrate, 2 g lecithin, 0.5 g yeast extract, 15 g agar, and 1000 mL distilled water.

[0032] The NBRIP medium formula is as follows: 10 g glucose, 0.1 g ammonium sulfate, 5 g magnesium chloride, 0.2 g potassium chloride, 0.25 g magnesium sulfate heptahydrate, 5 g calcium phosphate, 15 g agar, and 1000 mL distilled water.

[0033] The CAS detection medium formula is as follows: 0.0605 g of Chromium Azurite S (CAS), 0.0729 g of hexadecyltrimethylammonium bromide (HDTMA), 0.002645 g of ferric chloride hexahydrate, 0.29525 g of sodium dihydrogen phosphate dihydrate, 0.12135 g of disodium hydrogen phosphate dodecahydrate, 0.125 g of ammonium chloride, 0.0375 g of potassium dihydrogen phosphate, 0.0625 g of sodium chloride, 15 g of agar, and 1000 mL of distilled water.

[0034] The siderophores produced by the strain have a strong iron ion chelating ability, which can improve the availability of iron in the rhizosphere environment and promote the absorption and utilization of iron by plants. At the same time, by competitively binding iron ions, they reduce the ability of pathogenic microorganisms to acquire iron, which is conducive to maintaining the balance of the rhizosphere microecology.

[0035] Example 1: Screening of bacterial strains Healthy and plump alfalfa seeds of the Gannong 3 variety, widely cultivated in Northwest China and known for its high yield and quality, were selected. First, surface disinfection was performed to remove non-endophytic microorganisms. Specifically, the seeds were disinfected with 5% sodium hypochlorite for 10 min, rinsed with sterile water, and then disinfected with 75% ethanol for 3 min. Finally, all disinfected seeds were rinsed three times with sterile water to remove any residual disinfectant. 100 μL of the final rinse water was spread onto LB agar plates and incubated at 28°C for 7 days to verify the effectiveness of surface disinfection. The effectively disinfected seeds were sown in glass tissue culture tubes (48 mm × 200 mm) containing 100 mL of 1 / 2 MS solid medium and cultured in a greenhouse (16 h light / 8 h dark). After 28 days, the aseptically cultured seedlings were cut into fragments and homogenized using a sterile mortar and pestle in 10 mM MgCl2 solution. After serial dilutions, 100 μL of each dilution was spread onto three different culture media (LB, TSB, and R2A) and incubated at 30°C for 15–25 days to obtain as many microbial colonies as possible. During this period, colonies were picked and transferred to LB plates for isolation and purification based on colony morphology and color. Pure culture isolates were stored in 30% sterile glycerol at -80°C.

[0036] After purification, three strains were obtained, with colony morphology as follows: Figure 1 The colonies of strain 1 are raised, with a smooth surface and neat edges, and are grayish-white in color; this strain is designated X1. The colonies of strain 2 are raised, with a smooth and rounded surface, and are white in color; this strain is designated L5. The colonies of strain 3 are raised, with a smooth surface, and are pale yellow in color; this strain is designated Y39.

[0037] 2. Identification The full-length 16S rRNA gene of the isolate was amplified using universal bacterial primers 27F and 1492R. The PCR system and thermal cycling conditions were prepared according to the PCR Mix manufacturer's instructions. The PCR products were sequenced using Sanger sequencing. After splicing and trimming low-quality bases at both ends, the sequences were compared with the NCBI rRNA / ITS database to construct a phylogenetic tree and obtain the strain's taxonomic information.

[0038] 3. Results Phylogenetic tree of strains X1, L5 and Y39 as follows Figure 2 As shown. The 16S rRNA gene sequence of strain X1 is shown in SEQ ID No. 1, and it matches the sequence in the NCBI rRNA / ITS database. Acinetobacter lwoffii Since the homology reached 100% and the strain clustered on the phylogenetic tree, strain X1 can be identified as Acinetobacter roux, and it is named Acinetobacter roux. Acinetobacter lwoffii X1 was deposited on June 10, 2026 at the China Center for Type Culture Collection (CCTCC M 20261263), located at Wuhan University, Wuhan, Hubei Province, China. The contact number is 027-68754052.

[0039] The 16S rRNA gene sequence of strain L5 is shown in SEQ ID No. 2, and it is consistent with the sequence in the NCBI rRNA / ITS database. Pseudomonas hunanensis With 99.70% homology and clustering on the phylogenetic tree, strain L5 can be identified as *Pseudomonas hunanense*, and is named *Pseudomonas hunanense*. Pseudomonas hunanensis L5 was deposited on June 10, 2026 at the China Center for Type Culture Collection (CCTCC M 20261264), located at Wuhan University, Wuhan, Hubei Province, China. The contact number is 027-68754052.

[0040] The 16S rRNA gene sequence of strain Y39 is shown in SEQ ID No. 3, and it is consistent with the sequence in the NCBI rRNA / ITS database. Pseudomonas ceruminum With 99.85% homology and clustering on the phylogenetic tree, strain Y39 can be identified as... Pseudomonas ceruminum Name it Pseudomonas ceruminum Y39 was deposited on June 10, 2026 at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 20261268. The depository address is Wuhan University, Wuhan, Hubei Province, China. The contact number is 027-68754052.

[0041] In the following embodiments, Acinetobacter rumeni (… Acinetobacter lwoffiiX1 is abbreviated as Acinetobacter ruvidii X1; Pseudomonas hunanense ( Pseudomonas hunanensis L5 is abbreviated as Hunan Pseudomonas L5.

[0042] Example 2: Interstrain Compatibility Determination Acinetobacter rumenella X1, Pseudomonas hunanense L5 and Pseudomonas ceruminum Y39 strains were streaked in pairs on the same LB plate and incubated at 30°C for 48 hours. The presence of antagonistic zones was observed to evaluate the compatibility between strains.

[0043] Depend on Figure 3 It can be seen that no antagonistic regions were generated among the three strains, indicating that the strains can coexist and can be used for the construction of subsequent synthetic microbial communities.

[0044] Example 3: Determination of the growth-promoting ability of the strain Acinetobacter rumenella X1, Pseudomonas hunanense L5 and Pseudomonas ceruminum Y39 samples were inoculated into LB liquid medium and cultured at 30°C for 24 hours to obtain seed fermentation broth. The obtained seed fermentation broth was then inoculated into 100 mL of LB liquid medium at a volume ratio of 1%, and placed on a shaker at 30°C and 180 r·min. -1 Shake, culture alone for 48 hours, centrifuge at 4000 rpm for 10 minutes, collect the bacterial cells, resuspend in sterile water and adjust OD. 600 Mix equal amounts of the solution to 0.2, and obtain single-cell and synthetic bacterial suspensions respectively.

[0045] 1. Test of the strain's ability to solubilize organophosphates 10 μL of each of the above bacterial suspensions were inoculated onto Monkina organic phosphorus medium plates and incubated at 30°C for 5 days. The organic phosphorus solubilization capacity of the strains or synthetic bacterial groups was evaluated by measuring the ratio of the diameter of the transparent zone (D) to the diameter of the colony (d) (D / d).

[0046] The results are as follows Figure 4 As shown, Hunan Pseudomonas L5 and Pseudomonas ceruminum Y39 strains all possess the ability to decompose organophosphates, and the decomposition capacity of individual strains and the synthetic colony SynComY is comparable.

[0047] 2. Test of the strain's ability to solubilize inorganic phosphorus 10 μL of each of the above bacterial suspensions were inoculated onto NBRIP inorganic phosphorus medium plates and incubated at 30°C for 5 days. The inorganic phosphorus solubility of the strains or synthetic bacterial groups was assessed by measuring the ratio of the diameter of the transparent zone (D) to the diameter of the colony (d) (D / d).

[0048] The results are as follows Figure 5As shown, both Acinetobacter rumeniella X1 and Pseudomonas hunanense L5 possess the ability to solubilize inorganic phosphorus. However, compared with single strains, SynComY exhibits a significantly enhanced ability to solubilize inorganic phosphorus, indicating that strain combinations can improve the ability to solubilize inorganic phosphorus.

[0049] 3. Test of the strain's ability to produce siderophores 10 μL of each of the above bacterial suspensions were inoculated onto CAS detection medium plates and incubated at 30°C for 5 days. The siderophore-producing capacity of the strains or synthetic flora was assessed by measuring the ratio of the siderophore secretion zone diameter (D) to the colony diameter (d) (D / d). The CAS detection medium formulation was as follows: 0.0605 g of Chromium Azurite S (CAS), 0.0729 g of hexadecyltrimethylammonium bromide (HDTMA), 0.002645 g of ferric chloride hexahydrate, 0.29525 g of sodium dihydrogen phosphate dihydrate, 0.12135 g of disodium hydrogen phosphate dodecahydrate, 0.125 g of ammonium chloride, 0.0375 g of potassium dihydrogen phosphate, 0.0625 g of sodium chloride, 15 g of agar, and 1000 mL of distilled water.

[0050] The results are as follows Figure 6 As shown, Hunan Pseudomonas L5 and Pseudomonas ceruminum All Y39 strains possess the ability to produce siderophores, with SynComY exhibiting a siderophore-producing capacity comparable to that of a single strain.

[0051] Depend on Figure 4 , 5 As shown in section 6, each strain exhibited at least one plant growth-promoting activity. When the single strains were combined into the synthetic microbial community SynComY, the growth-promoting activity of SynComY encompassed that of the individual strains that constituted it, emphasizing the complementarity of functions among the individual strains within the synthetic microbial community. Therefore, subsequent experiments used the synthetic microbial community SynComY as the test subject.

[0052] Example 4: Preparation of inoculant and its use in pot experiments on alfalfa 1. Preparation of microbial agents Acinetobacter rumenella X1, Pseudomonas hunanense L5 and Pseudomonas ceruminum Y39 strain was inoculated into LB liquid medium and cultured at 30℃ for 24 h to obtain seed fermentation broth. The obtained seed fermentation broth was inoculated into 100 mL LB liquid medium at a volume ratio of 1%, and placed on a shaker at 30℃ and 180 rpm for 48 h. After centrifugation at 4000 rpm for 10 minutes, the bacterial cells were collected, resuspended in sterile water, and the OD600 was adjusted to 0.2. The cells were then mixed in equal volumes to obtain the liquid bacterial agent.

[0053] 2. Pot experiment of microbial agent Alfalfa seeds were surface-sterilized as described above and germinated for 2 days in the dark on moist, sterile filter paper. Uniform seeds were selected and sown in flowerpots (10 × 8.5 cm) containing 400g of dry soil that had passed through a 2mm sieve. The experiment was divided into two groups: an experimental group treated with a microbial agent and a control group without. Each group had 6 biological replicates. Before sowing, alfalfa seeds were soaked in a corresponding synthetic microbial suspension for 30 minutes; uninoculated seeds were soaked in sterile water. At sowing, 10 mL of synthetic microbial suspension was applied to each pot as the treatment group, while the control group received an equal volume of sterile water. After germination, 3 seedlings were retained per pot. 14 days later, 20 mL of synthetic microbial suspension (OD600 = 0.2) or sterile water was applied again to the roots of the alfalfa. The greenhouse was maintained at 28℃ for 16 hours of light and 24℃ for 8 hours of darkness, with the flowerpots being adjusted periodically. Samples were harvested 30 days after planting, the length of alfalfa plants was measured, and the plants were dried at 80℃ for 48 hours to constant weight before the biomass was measured.

[0054] Depend on Figure 7 It was found that application of SynComY significantly promoted alfalfa growth, especially in terms of plant height and biomass. After inoculation with SynComY, the aboveground dry weight, total plant dry weight, and plant height of alfalfa increased by 51.29%, 55.80%, and 27.54%, respectively, all of which were significant.

[0055] In summary, this invention provides a single-strain Acinetobacter ruthenii with growth-promoting effects. Acinetobacter lwoffii X1, Hunan Pseudomonas ( Pseudomonas hunanensis L5 and Pseudomonas ceruminum Y39 was deposited at the China Center for Type Culture Collection (CCTCC) on June 10, 2026, with accession numbers CCTCC M20261263, CCTCC M 20261264, and CCTCC M 20261268, respectively. A compound microbial agent composed of the above-mentioned single bacteria was provided, which significantly promoted plant growth, significantly increased alfalfa dry matter accumulation, and improved alfalfa plant height. The compound microbial agent SynComY constructed in this invention consists of Acinetobacter rumeniella X1, Pseudomonas hunanense L5, and... Pseudomonas cerumenComposed of Y39, all three strains possess plant growth-promoting functions, including the ability to produce siderophores and activate phosphorus. Furthermore, the strains in SynComY also have the ability to dissolve or mineralize soil phosphorus, converting inorganic and organic phosphorus in the soil that are difficult for plants to directly utilize into absorbable and usable phosphorus, thus improving phosphorus utilization efficiency. Specifically, inorganic phosphorus activation mainly promotes the dissolution of insoluble phosphates through the secretion of metabolic products such as organic acids; organic phosphorus activation mainly relies on the secretion of extracellular enzymes such as phosphatases to promote the mineralization and release of organophosphorus compounds. These functions work together to enhance alfalfa's ability to acquire key nutrients, promoting plant growth and biomass accumulation. Therefore, SynComY of this invention can synergistically exert its growth-promoting effects through multiple mechanisms such as plant hormone regulation, iron activation, and phosphorus activation, providing continuous nutritional support for alfalfa growth. It can also be used to prepare biofertilizers with phosphorus-solubilizing and organophosphorus pesticide degradation functions, demonstrating significant agricultural application value.

[0056] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A type of Acinetobacter rumenella ( Acinetobacter lwoffii X1, characterized in that, It was deposited at the China Center for Type Culture Collection on June 10, 2026, with accession number CCTCC M 20261263.

2. A type of Hunan Pseudomonas ( Pseudomonas hunanensis L5, characterized in that, It was deposited at the China Center for Type Culture Collection on June 10, 2026, with accession number CCTCC M 20261264.

3. A kind Pseudomonas ceruminis Y39, characterized in that, It was deposited at the China Center for Type Culture Collection on June 10, 2026, with accession number CCTCC M 20261268.

4. A growth-promoting agent, SynComY, characterized in that, The growth-promoting agent SynComY includes Acinetobacter rumeni (… Acinetobacter lwoffii X1, Hunan Pseudomonas ( Pseudomonas hunanensis L5 and Pseudomonas ceruminis Y39 was deposited at the China Center for Type Culture Collection on June 10, 2026, with accession numbers CCTCC M 20261263, CCTCC M 20261264, and CCTCC M 20261268, respectively.

5. The growth-promoting bacterial agent SynComY as described in claim 4, characterized in that, The growth-promoting agent SynComY is composed of Acinetobacter rumeni (… Acinetobacter lwoffii X1, Hunan Pseudomonas ( Pseudomonas hunanensis L5 and Pseudomonas ceruminis Composed of Y39.

6. The Acinetobacter rumenella X1 as described in claim 1, the Pseudomonas hunanense L5 as described in claim 2, or the [unclear] as described in claim 3 Pseudomonas ceruminis The application of SynComY, the growth-promoting microbial agent as described in claim 4, in promoting plant growth.

7. The Acinetobacter rumenella X1 as described in claim 1, the Pseudomonas hunanense L5 as described in claim 2, or the [unclear] as described in claim 3 Pseudomonas ceruminis Application of SynComY, the growth-promoting microbial agent according to claim 4, in the preparation of products for increasing plant biomass.

8. The Acinetobacter rumenella X1 as described in claim 1, the Pseudomonas hunanense L5 as described in claim 2, or the [unclear] as described in claim 3 Pseudomonas ceruminis The application of SynComY, the growth-promoting bacterial agent as described in claim 4, in formulations that improve the absorption of phosphorus / iron by plant roots.

9. The *Pseudomonas hunanensis* L5 as described in claim 2, or the strain as described in claim 3. Pseudomonas ceruminis Application of SynComY, the growth-promoting microbial agent as described in claim 4, in the remediation of soil / water bodies contaminated with organophosphorus pesticides.

10. The *Pseudomonas hunanensis* L5 as described in claim 2, or the strain as described in claim 3. Pseudomonas ceruminis Application of SynComY, the growth-promoting microbial agent as described in claim 4, in the preparation of biofertilizers with phosphorus solubilizing and organophosphorus pesticide degradation functions.

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