A device and method for quantitative detection of trace proteins

Abstract

The application discloses a kind of quantitative detection device and method of trace protein, belongs to protein detection device technical field, and its quantitative detection device and method of trace protein include lower shell, the upper end of the lower shell is fixedly installed with upper shell, the inner cavity middle part of the upper shell is fixedly installed with detection main body, the side of the upper shell in detection main body is fixedly installed drop measurement piece, the upper end of the drop measurement piece is rotatably provided with micro liquid detection head, the side of the upper shell in micro liquid detection head is rotatably provided with turnover cover, the lower side of the front end of the micro liquid detection head is fixedly installed with micro drop optical path, by being provided with the structure of sealing cap, when test tube is arranged in the inside of detection tube cover, when the inside of test tube is detected, liquid in its inside is divided to the upper end of drop measurement plate by capillary, simultaneously detection and correction detection are carried out, manual pipetting is not needed, and protein in liquid can be rapidly detected.

Description

Technical Field

[0001] This invention relates to the field of protein detection device technology, and more specifically, to a device and method for quantitative detection of trace proteins. Background Technology

[0002] Quantitative detection of trace proteins is a core testing method in the fields of biomedical testing, clinical urine testing, biochemical sample analysis, and pathological screening. Accurate measurement of trace protein concentrations is a crucial reference for assessing renal function, inflammatory markers, metabolic status, and pathological changes in the human body. Compared to samples used in routine biochemical tests, trace protein samples generally exhibit extremely low sample volume, weak protein concentration, weak signal response, susceptibility to loss and contamination, and numerous interfering factors. This places extremely high demands on the sampling accuracy, optical path stability, signal acquisition accuracy, and anti-interference capabilities of the testing equipment.

[0003] Traditional whole-tube protein detection devices rely on the entire tube's light transmission path to detect absorbance. Their advantages include no sample aliquoting, no sample loss, and rapid in-situ detection. However, this detection mode has significant accuracy limitations. Because trace protein samples have extremely low concentrations and weak optical signals, the whole-tube detection process is greatly affected by tube wall refraction, uneven solution thickness, sedimentation of impurities at the bottom, and light path scattering interference. In the low-concentration range, signal identification is poor, and baseline drift is severe, easily leading to problems such as low detection values, poor data repeatability, missed detections, and false detections of trace proteins. It cannot achieve high-precision quantitative detection and is only suitable for preliminary screening of high-concentration protein samples, completely unsuitable for precise quantitative testing of trace and ultra-trace proteins.

[0004] Another mainstream detection method is external manual droplet detection. This method uses a pipette or external dripping device to transfer the sample from the test tube to the detection slide for micro-droplet optical detection. This method can reduce the thickness of the detection liquid layer, reduce optical path interference, and improve the detection accuracy of weak signals. It is currently the main means of high-precision detection of trace proteins. However, this type of detection structure and process has many unavoidable technical defects. The manual external pipetting and dropping process is prone to causing loss and contamination of trace samples. At the same time, it is easy to generate problems such as air bubbles and uneven droplet thickness, which directly affect the spectral detection accuracy. It relies on external pipetting equipment and manual operation, which is cumbersome, has low detection efficiency, large human operation error, and poor detection consistency.

[0005] Existing microprotein detection equipment has a single structure and function, lacking an independently controllable micro-dispensing structure and a dual-mode collaborative detection system. It cannot meet the needs of modern clinical precision medicine, standardized, high-precision, and traceable quantitative detection of large batches of micro samples. Therefore, it is necessary to design a microprotein quantitative detection device and method that combines non-destructive whole-tube in-situ detection, automated and precise micro-droplet dispensing, and dual-mode data fusion correction to solve the many technical drawbacks of existing technologies. Summary of the Invention

[0006] The purpose of this invention is to provide a device and method for quantitative detection of trace proteins to solve the problems mentioned in the background art.

[0007] A device and method for quantitative detection of trace proteins includes a lower shell, an upper shell fixedly installed at the upper end of the lower shell, a detection body fixedly installed in the middle of the inner cavity of the upper shell, a dropper fixedly installed on one side of the upper shell located on the detection body, a micro-liquid detection head rotatably disposed at the upper end of the dropper, a flip-top cover rotatably disposed on the side of the micro-liquid detection head located on the upper shell, a microdroplet optical path fixedly installed on the lower front end of the micro-liquid detection head, and a dropper plate fixedly installed at the position of the dropper located on the microdroplet optical path. A detection tube sleeve is fixedly installed in the middle of the detection body. A sealing cap is provided at the upper end of the detection tube sleeve. A pressing membrane is fixedly installed in the middle of the sealing cap. An elastic diaphragm is provided in the middle of the pressing membrane. A capillary tube is fixedly installed on the inner side of the sealing cap. A reinforcing member is fixedly installed on the side of the sealing cap located on the capillary tube. A light generator is fixedly installed on the rear side of the upper housing, a detection optical path is fixedly installed on the front side of the light generator, and a first detector is fixedly installed on the detection body at a position symmetrical to the detection optical path.

[0008] Furthermore, an isolation chamber is fixedly installed in the middle of the inner cavity of the lower housing, a battery slot is fixedly installed on the lower side of the inner cavity of the isolation chamber, and a secondary mounting plate is rotatably provided on the lower side of the battery slot.

[0009] By adopting the above technical solution, this device can be equipped with multiple parallel batteries to power the whole device, which facilitates rapid detection of samples.

[0010] Furthermore, the measuring element has a detection groove on the lower side of the lower housing, and the detection groove is an arc-shaped groove structure.

[0011] By adopting the above technical solution, the arc-shaped groove structure can facilitate the detection of internal liquids by the microdroplet optical path, and at the same time, it is convenient for operators to use a pipette to perform droplet detection on a small amount of test sample.

[0012] Furthermore, the droplet measuring element has a connecting groove on the side of the detection groove, and the detection body has a micro-droplet detection plate at the lower end of the droplet measuring plate.

[0013] By adopting the above technical solution, the connecting groove on the side of the droplet measuring piece can facilitate the setting of the reinforcing piece, and the microdroplet detection plate can integrate and analyze the signal received by the upper droplet measuring plate.

[0014] Furthermore, the upper end of the pressing membrane is an arc-shaped rubber membrane structure, the lower inner wall of the pressing membrane is provided with a threaded groove, the side of the elastic diaphragm is provided with a corresponding thread, and the middle part of the elastic diaphragm is an upwardly bulging rubber membrane.

[0015] By adopting the above technical solution, the upper end of the pressing membrane can be pressed. When the pressing membrane is squeezed downward, the pressure between the pressing membrane and the elastic diaphragm increases. The pressure will squeeze the elastic diaphragm downward, thereby increasing the internal pressure of the lower test tube.

[0016] Furthermore, a main control screen is fixedly installed on the front side of the upper and lower housings, and a heat sink is fixedly installed on the rear side of the upper housing.

[0017] By adopting the above technical solution, the main control screen can record and analyze the detection results of the first detector and the microdroplet detection plate, which facilitates data collection by operators.

[0018] Furthermore, the detection optical path and the detection body are located on the lower side of the detection tube sleeve, the detection tube sleeve is a transparent PVC material component, and the detection tube sleeve has a corresponding circular through groove at the position of the detection optical path and the detection body.

[0019] By adopting the above technical solution, when the test tube containing the test liquid is placed inside the test tube sleeve, the liquid inside the test tube can be optically detected through the detection optical path.

[0020] Furthermore, a heat sink is fixedly installed in the middle of the lower housing, and a main control board is fixedly installed at the upper end of the heat sink.

[0021] By adopting the above technical solution, the main control board is electrically connected to the internal first detector and microdroplet detection board. After processing the data, the main control board will display it on the main control screen.

[0022] Furthermore, it includes the following steps; Step 1: Turn on the device power and start the device self-test through the main control screen. The light generator, detection optical path, first detector, and micro-liquid detection head will complete the optical path baseline calibration and zero point correction in sequence. Open the flip cover and place the test tube containing the micro-protein test solution inside the detection tube sleeve in the middle of the detection body. The detection tube sleeve is made of transparent PVC material to ensure that the optical path is unobstructed and complete the test tube limit fixation.

[0023] Step 2: Place the sealing cap on the upper end of the test tube to completely seal the upper end of the test tube, forming a closed cavity inside the test tube. A pressing membrane and an elastic diaphragm are set in the middle of the sealing cap. The capillary tube is fixed on the side of the inner cavity of the sealing cap. The capillary tube is limited and reinforced by the reinforcing member to prevent the pipeline from shifting and leaking, and to ensure the airtightness of the cavity and the stability of the liquid conduction.

[0024] Step 3: The device starts the whole tube detection mode. The light generator emits a detection light source. The light passes through the detection light path, through the detection tube sleeve and the solution to be tested inside. The symmetrically arranged first detector collects the absorbance spectrum signal of the whole tube solution in real time and transmits the detection signal to the main control board to complete the in-situ basic detection of the whole trace protein solution inside the test tube and obtain the basic parameters of the whole tube sample concentration.

[0025] Step 4: When the flip-top is closed, it will squeeze the pressing membrane. The pressing membrane is an arc-shaped rubber membrane structure. When pressed, it squeezes the elastic diaphragm that bulges upward on the inside, causing the elastic diaphragm to press down and increase the pressure on the sealed cavity inside the test tube. The micro-protein solution inside the test tube, under the action of air pressure, is automatically discharged upward along the capillary on the side of the sealing cap, squeezing out a micro-droplet of 0.5~5μL, which is accurately delivered to the surface of the dispensing plate at the top of the dispensing device, forming a uniform micro-droplet. No manual liquid transfer or external drip irrigation equipment is required, realizing autonomous and controllable micro-dispensing.

[0026] Step 5: Rotate and adjust the micro-liquid detection head to the working detection position. Perform high-precision spectral detection on the micro-droplet on the surface of the test plate through the micro-droplet optical path. Combined with the lower micro-droplet detection plate, complete the signal-assisted acquisition. Utilize the characteristics of small droplet volume, stable optical path, and low impurity interference to obtain high-precision micro-protein detection data, making up for the deficiency of weak low-concentration signals in whole-tube detection.

[0027] Step 6: The main control board has a built-in data processing program that compares, fits, and corrects the basic data of the whole tube in situ detection obtained in Step 3 with the high-precision microdroplet detection data obtained in Step 5. Combined with the preset standard protein curve, the detection error is automatically corrected, and the precise micro-protein concentration of the sample to be tested is finally calculated.

[0028] After step 7 is completed, open the sealed cap and take out the test tube. If there is not much loss of the sample, it can be retained for retesting, thus completing the single-test micro-protein quantification process.

[0029] Compared with the prior art, the advantages of this invention are: In this invention, by setting a structure with a sealing cap, when the test tube is placed inside the detection tube sleeve, the liquid inside the test tube will be separated to the upper end of the dispensing plate through the capillary tube when the test tube is being tested, and the detection and correction detection can be performed simultaneously. No manual liquid transfer is required, and the protein in the liquid can be detected quickly. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 These are two structural schematic diagrams of the overall structure of the present invention; Figure 3This is a cross-sectional view of the overall structure of the present invention; Figure 4 This is a schematic diagram of the structure of the detection body of the present invention; Figure 5 This is a cross-sectional view of the detection sleeve of the present invention.

[0031] The following are the labeling instructions in the diagram: 1. Upper shell; 101. Droplet measuring element; 102. Connecting groove; 103. Detection groove; 2. Lower shell; 3. Main control screen; 4. Flip cover; 5. Micro-liquid detection head; 501. Microdroplet optical path; 502. Droplet measuring plate; 6. Secondary mounting plate; 7. Heat sink; 8. Detection body; 9. First detector; 10. Sealing cap; 1001. Detection tube sleeve; 1002. Elastic diaphragm; 1003. Pressing membrane; 11. Detection optical path; 12. Light generator; 13. Main control board; 14. Heat sink; 15. Isolation chamber; 16. Battery slot; 17. Battery; 18. Capillary tube; 19. Reinforcing element; 20. Microdroplet detection plate. Detailed Implementation

[0032] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] like Figure 1 - Figure 5 As shown, the embodiment of the present invention provides: including a lower shell 2, an upper shell 1 fixedly installed at the upper end of the lower shell 2, a detection body 8 fixedly installed in the middle of the inner cavity of the upper shell 1, a dropper 101 fixedly installed on one side of the upper shell 1 located on the detection body 8, a micro-liquid detection head 5 rotatably disposed at the upper end of the dropper 101, a flip cover 4 rotatably disposed on the side of the upper shell 1 located on the micro-liquid detection head 5, a micro-droplet optical path 501 fixedly installed on the lower side of the front end of the micro-liquid detection head 5, and a dropper plate 502 fixedly installed on the dropper 101 at the position of the micro-droplet optical path 501; A detection tube sleeve 1001 is fixedly installed in the middle of the detection body 8. A sealing cap 10 is provided at the upper end of the detection tube sleeve 1001. A pressing membrane 1003 is fixedly installed in the middle of the sealing cap 10. An elastic diaphragm 1002 is provided in the middle of the pressing membrane 1003. A capillary tube 18 is fixedly installed on the side of the inner cavity of the sealing cap 10. A reinforcing member 19 is fixedly installed on the side of the sealing cap 10 located on the capillary tube 18. A light generator 12 is fixedly installed on the rear side of the upper housing 1 and the detection light path 11 is fixedly installed on the front side of the light generator 12. A first detector 9 is fixedly installed on the detection body 8 at a symmetrical position to the detection light path 11. An isolation chamber 15 is fixedly installed in the middle of the inner cavity of the lower housing 2. A battery slot 16 is fixedly installed on the lower side of the inner cavity of the isolation chamber 15. A secondary mounting plate 6 is rotatably installed on the lower side of the battery slot 16. This device can be equipped with multiple parallel batteries 17 to power the whole device, which facilitates the device to quickly detect samples. The droplet measuring element 101 has a detection groove 103 on the lower side of the lower housing 2. The detection groove 103 has an arc-shaped groove structure. The arc-shaped groove structure can facilitate the micro-droplet optical path 501 to detect the liquid inside, and at the same time, it is convenient for operators to use a pipette to perform droplet detection on a small amount of test sample. The droplet measuring element 101 has a connecting groove 102 on the side of the detection groove 103. The detection body 8 has a micro-droplet detection plate 20 at the lower end of the droplet measuring plate 502. The connecting groove 102 on the side of the droplet measuring element 101 can facilitate the setting of the reinforcing element 19. The micro-droplet detection plate 20 can integrate and analyze the signals received by the upper droplet measuring plate 502. The upper end of the pressing membrane 1003 is an arc-shaped rubber membrane structure. The lower inner wall of the pressing membrane 1003 is provided with a threaded groove. The side of the elastic diaphragm 1002 is provided with a corresponding thread. The middle part of the elastic diaphragm 1002 is an upwardly bulging rubber membrane. The upper end of the pressing membrane 1003 can be pressed. When the pressing membrane 1003 is squeezed downward, the pressure between the pressing membrane 1003 and the elastic diaphragm 1002 increases. The pressure will squeeze the elastic diaphragm 1002 downward, thereby increasing the internal pressure of the lower test tube. The main control screen 3 is fixedly installed on the front side of the upper housing 1 and the lower housing 2, and the heat sink 7 is fixedly installed on the rear side of the upper housing 1. The main control screen 3 can record and analyze the detection results of the first detector 9 and the microdroplet detection plate 20, which facilitates data collection by the operator. The detection optical path 11 and the detection body 8 are located on the lower side of the detection tube sleeve 1001. The detection tube sleeve 1001 is a transparent PVC material component. The detection tube sleeve 1001 has a corresponding circular through groove at the position of the detection optical path 11 and the detection body 8. When the test tube containing the detection liquid is placed inside the detection tube sleeve 1001, the liquid inside the test tube can be optically detected through the detection optical path 11. A heat sink 14 is fixedly installed in the middle of the lower housing 2, and a main control board 13 is fixedly installed on the upper end of the heat sink 14. The main control board 13 is electrically connected to the first detector 9 and the microdroplet detection board 20 inside. After the main control board 13 processes the data, it will be displayed on the main control screen 3. Step 1: Turn on the device power and start the device self-test through the main control screen 3. The light generator 12, detection optical path 11, first detector 9, and micro-liquid detection head 501 sequentially complete the optical path baseline calibration and zero point correction. Open the flip cover 4 and place the test tube containing the micro-protein test solution inside the detection tube sleeve 1001 in the middle of the detection body 8. The detection tube sleeve 1001 is made of transparent PVC material to ensure that the optical path is unobstructed and complete the test tube limit fixation.

[0034] Step 2: Cover the upper end of the test tube with the sealing cap 10. The sealing cap 10 completely seals the upper end of the test tube, forming a closed cavity inside the test tube. The sealing cap 10 has a pressing membrane 1003 and an elastic diaphragm 1002 in the middle. The capillary tube 18 is fixed to the side of the inner cavity of the sealing cap 10. The capillary tube 18 is limited and reinforced by the reinforcing member 19 to prevent the pipeline from shifting and leaking, and to ensure the airtightness of the cavity and the stability of the liquid conduction.

[0035] Step 3: The device starts the whole tube detection mode. The light generator 12 emits a detection light source. The light passes through the detection light path 11, through the detection tube sleeve 1001 and the solution to be tested inside. The symmetrically arranged first detector 9 collects the absorbance spectrum signal of the whole tube solution in real time and transmits the detection signal to the main control board 13 to complete the in-situ basic detection of the whole trace protein solution inside the test tube and obtain the basic parameters of the whole tube sample concentration.

[0036] Step 4: When the flip cover 4 is closed, it will squeeze the pressing membrane 1003. The pressing membrane 1003 is an arc-shaped rubber membrane structure. After being pressed, it squeezes the elastic diaphragm 1002 that bulges upward on the inner side, causing the elastic diaphragm 1002 to press down and increase the pressure on the sealed cavity inside the test tube. The micro-protein solution inside the test tube, after being pressurized, is automatically discharged upward along the capillary 18 on the side of the sealing cap 10 under the action of air pressure, squeezing out 0.5~5μL micro-droplets, which are accurately delivered to the surface of the dispensing plate 502 at the upper end of the dispensing element 101, forming uniform micro-droplets. No manual liquid transfer or external drip irrigation equipment is required, realizing autonomous and controllable micro-dispensing.

[0037] Step 5: Rotate and adjust the micro-liquid detection head 5 to the working detection position. Perform high-precision spectral detection on the micro-droplets on the surface of the droplet plate 502 through the micro-droplet optical path 501. Combined with the lower micro-droplet detection plate 20, complete the signal auxiliary acquisition. Utilize the characteristics of small droplet volume, stable optical path, and low impurity interference to obtain high-precision micro-protein detection data and make up for the deficiency of weak low-concentration signals in whole-tube detection.

[0038] Step 6: The main control board 13 has a built-in data processing program that compares, fits, and corrects the basic data of the whole tube in situ detection obtained in step 3 with the high-precision microdroplet detection data obtained in step 5. Combined with the preset standard protein curve, the detection error is automatically corrected, and the precise micro-protein concentration of the sample to be tested is finally calculated.

[0039] After step 7 is completed, open the sealing cap 10 and take out the test tube. If there is no significant loss of the sample, it can be retained for retesting, thus completing the single-test micro-protein quantification process.

[0040] Working principle of this invention: The device is assembled from an upper shell 1 and a lower shell 2 to form a closed protective body. An isolation chamber 15 is set in the inner cavity of the lower shell 2, and a battery slot 16 is opened at the bottom of the isolation chamber 15. A secondary mounting plate 6 is rotatably assembled below the battery slot 16. Multiple sets of batteries 17 can be installed inside the battery slot 16 to power the whole machine. A heat sink 14 is fixed in the middle of the lower shell 2, and the heat sink 14 supports the main control board 13. A heat sink 7 is installed on the rear side of the upper shell 1. During the operation of the whole machine, the heat generated by the main control board 13, the light generator 12 and other heat-generating components can be discharged to the outside through the heat sink 14 and the heat sink 7 to avoid the internal high temperature from interfering with the optical path detection accuracy. A main control screen 3 is installed on the front of the device. The main control screen 3 is electrically connected to the main control board 13 and is used for human-machine interaction, equipment self-test control, real-time display and storage of detection data. A detection body 8 is fixed in the middle of the inner cavity of the upper shell 1. A transparent PVC is installed in the center of the detection body 8. The detection tube sleeve 1001 is made of a special material. A circular groove is formed on the side wall of the detection tube sleeve 1001 to ensure unobstructed light transmission. During testing, the flip-top cover 4 is opened, and the test tube containing a trace amount of protein test solution is vertically placed inside the detection tube sleeve 1001 for positioning and fixation. Then, the sealing cap 10 is fastened to the test tube opening, forming a completely sealed cavity. A capillary tube 18 is fixed to the inner side of the sealing cap 10, and a reinforcing member 19 is provided beside the capillary tube 18. The reinforcing member 19 limits and reinforces the capillary tube 18, preventing displacement and leakage of the liquid delivery line, ensuring the cavity's airtightness and the stability of the trace liquid delivery. The sealing cap 10 has a threaded connection in the middle with a pressing membrane 1003 and an elastic diaphragm 1002. The upper end of the pressing membrane 1003 is an arc-shaped rubber structure, and the middle of the elastic diaphragm 1002 protrudes upward. The two are connected by a threaded seal to form a sealed pressurizing assembly. After the sample is sealed, the device starts the whole tube detection program. The light generator 12 on the rear side of the detection body 8 emits a stable detection light source. The light is transmitted forward to the detection light path 11. The light passes through the through groove on the side wall of the detection tube sleeve 1001 and penetrates all the test solution inside the test tube. The first detector 9, symmetrically arranged on the other side of the detection body 8, simultaneously collects the absorbance spectrum signal of the solution. The first detector 9 transmits the collected whole tube spectrum basic signal to the main control board 13 in real time to complete the in-situ basic detection of the test tube original solution without loss, and obtain the basic parameters of the initial concentration of the sample. The whole process does not require splitting or loss of the test sample. The original solution in the test tube is completely retained after the detection. During the test, the rotating and flipping cap 4 presses down on the pressing membrane 1003 at the top of the sealing cap 10 when the sealing cap 10 is fixed. The pressing membrane 1003 deforms under the downward pressure, squeezing the elastic diaphragm 1002 that bulges upward inside. The elastic diaphragm 1002 simultaneously compresses the air inside the sealing cap 10, and the air pressure in the sealed test tube cavity rises rapidly. Under the action of the internal air pressure, the trace amount of protein test solution in the test tube is guided upward along the capillary 18, and 0.5~5μL of trace droplets are accurately squeezed out. The liquid is guided by the dropper 101 and evenly drips onto the surface of the dropper plate 502 above the dropper 101. The entire liquid separation structure achieves automatic liquid separation by flipping mechanical linkage and air pressure boosting, without the need for pipettes or manual liquid dropping operations. After the micro-droplet separation is completed, a micro-droplet optical path 501 is fixed at the lower end of the micro-droplet detection head 5. The micro-droplet optical path 501 is vertically aligned with the micro-droplet on the surface of the dispensing plate 502 to perform high-precision spectral scanning detection on the droplet. A micro-droplet detection plate 20 is set below the dispensing plate 502. The micro-droplet detection plate 20 synchronously collects and integrates the weak spectral signal output by the micro-droplet optical path 501 and transmits it to the main control board 13. The micro-droplet liquid layer is thin and the light path scattering interference is low, which can capture low-concentration weak protein signals that cannot be identified by whole tube detection, making up for the lack of accuracy of whole tube detection. The main control board 13 has a built-in dedicated micro-protein... The white data processing program receives the basic detection data of the whole tube of original solution transmitted by the first detector 9 and the high-precision detection data of micro-droplets transmitted by the micro-droplet detection plate 20. The main control board 13 compares and fits the two sets of detection data, corrects the difference, retrieves the standard protein concentration correction curve stored in the device, automatically eliminates the detection errors caused by tube wall refraction, solution thickness, and baseline drift, and finally outputs the accurate micro-protein concentration value of the sample to be tested through algorithm correction calculation. The final detection result is synchronously transmitted to the main control screen 3 for visualization and storage. The entire detection process only diverts 0.5~5μL of micro-liquid for high-precision retesting. Most of the original solution to be tested is retained in the test tube without loss, which can be repeated for retesting and sample retention for traceability, completing a complete single micro-protein quantitative detection process.

[0041] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention.

Claims

1. A device for quantitative detection of trace proteins, comprising a lower housing (2), characterized in that: The upper shell (1) is fixedly installed on the upper end of the lower shell (2). The detection body (8) is fixedly installed in the middle of the inner cavity of the upper shell (1). The drop test element (101) is fixedly installed on one side of the upper shell (1) and the micro liquid detection head (5) is rotatably provided on the upper end of the drop test element (101). The flip cover (4) is rotatably provided on the side of the micro liquid detection head (5) on the upper shell (1). The micro drop optical path (501) is fixedly installed on the lower front end of the micro liquid detection head (5). The drop test plate (502) is fixedly installed on the position of the drop test element (101) at the position of the micro drop optical path (501). A detection tube sleeve (1001) is fixedly installed in the middle of the detection body (8). A sealing cap (10) is provided at the upper end of the detection tube sleeve (1001). A pressing membrane (1003) is fixedly installed in the middle of the sealing cap (10). An elastic diaphragm (1002) is provided in the middle of the pressing membrane (1003). A capillary tube (18) is fixedly installed on the inner side of the sealing cap (10). A reinforcing member (19) is fixedly installed on the side of the sealing cap (10) located on the capillary tube (18). The upper housing (1) is fixedly installed with a light generator (12) on the rear side of the detection body (8), and a detection optical path (11) is fixedly installed on the front side of the light generator (12). The detection body (8) is fixedly installed with a first detector (9) at a symmetrical position of the detection optical path (11).

2. The device for quantitative detection of trace proteins according to claim 1, characterized in that: An isolation chamber (15) is fixedly installed in the middle of the inner cavity of the lower housing (2). A battery slot (16) is fixedly installed on the lower side of the inner cavity of the isolation chamber (15). A secondary mounting plate (6) is rotatably provided on the lower side of the battery slot (16).

3. The quantitative detection device for trace proteins according to claim 1, characterized in that: The drop tester (101) has a detection groove (103) on the lower side of the lower housing (2), and the detection groove (103) is an arc-shaped groove structure.

4. The device for quantitative detection of trace proteins according to claim 1, characterized in that: The droplet measuring element (101) has a connecting groove (102) on the side of the detection groove (103), and the detection body (8) has a micro-droplet detection plate (20) at the lower end of the droplet measuring plate (502).

5. The quantitative detection device for trace proteins according to claim 1, characterized in that: The upper end of the pressing membrane (1003) is an arc-shaped rubber membrane structure, the lower inner wall of the pressing membrane (1003) is provided with a threaded groove, the side of the elastic diaphragm (1002) is provided with a corresponding thread, and the middle part of the elastic diaphragm (1002) is an upwardly bulging rubber membrane.

6. The quantitative detection device for trace proteins according to claim 1, characterized in that: The main control screen (3) is fixedly installed on the front side of the upper housing (1) and the lower housing (2), and the heat sink (7) is fixedly installed on the rear side of the upper housing (1).

7. The device for quantitative detection of trace proteins according to claim 1, characterized in that: The detection optical path (11) and the detection body (8) are located on the lower side of the detection tube sleeve (1001). The detection tube sleeve (1001) is a transparent PVC material component. The detection tube sleeve (1001) has a corresponding circular through groove at the position of the detection optical path (11) and the detection body (8).

8. The device for quantitative detection of trace proteins according to claim 1, characterized in that: A heat sink (14) is fixedly installed in the middle of the lower housing (2), and a main control board (13) is fixedly installed at the upper end of the heat sink (14).

9. A method for quantitative detection of trace proteins, relating to a device for quantitative detection of trace proteins as described in any one of claims 1-8, characterized in that... Includes the following steps; Step 1: Turn on the power of the device and start the device self-test through the main control screen (3). The light generator (12), detection optical path (11), first detector (9), and micro liquid detection head (501) complete the optical path baseline calibration and zero point correction in sequence. Open the flip cover (4) and place the test tube containing the micro protein test solution inside the detection tube sleeve (1001) in the middle of the detection body (8). The detection tube sleeve (1001) is made of transparent PVC material to ensure that the optical path is unobstructed and complete the test tube limit fixation. Step 2: Cover the upper end of the test tube with the sealing cap (10). The upper end of the test tube is completely sealed by the sealing cap (10), so that a closed cavity is formed inside the test tube. A pressing membrane (1003) and an elastic diaphragm (1002) are set in the middle of the sealing cap (10). The capillary tube (18) is fixed on the side of the inner cavity of the sealing cap (10). The capillary tube (18) is limited and reinforced by the reinforcing member (19) to prevent the pipeline from deviating and leaking, and to ensure the airtightness of the cavity and the stability of the liquid conduction. Step 3: The device starts the whole tube detection mode. The light generator (12) emits the detection light source. The light passes through the detection light path (11) and the detection tube sleeve (1001) and the internal test solution. The symmetrically arranged first detector (9) collects the absorbance spectrum signal of the whole tube solution in real time and transmits the detection signal to the main control board (13) to complete the in-situ basic detection of the whole trace protein solution inside the test tube and obtain the basic parameters of the whole tube sample concentration. Step 4 When the flip cover (4) is closed, the flip cover (4) will squeeze the pressing membrane (1003). The pressing membrane (1003) is an arc-shaped rubber membrane structure. After being pressed, it squeezes the elastic diaphragm (1002) that bulges upward on the inner side, so that the elastic diaphragm (1002) presses down to increase the pressure of the sealed cavity inside the test tube. The micro-protein solution inside the test tube after being pressed is automatically discharged upward along the capillary (18) on the side of the sealing cap (10) under the action of air pressure, squeezing out 0.5~5μL micro-droplets, which are accurately delivered to the surface of the drip plate (502) at the top of the drip test piece (101) to form uniform micro-droplets. No manual liquid transfer or external drip irrigation equipment is required, realizing autonomous and controllable micro-droplet distribution. Step 5: Rotate and adjust the micro-liquid detection head (5) to the working detection position. Perform high-precision spectral detection on the micro-droplet on the surface of the droplet plate (502) through the micro-droplet optical path (501). Combined with the lower micro-droplet detection plate (20), complete the signal-assisted acquisition. Utilize the characteristics of small droplet volume, stable optical path, and low impurity interference to obtain high-precision micro-protein detection data and make up for the deficiency of weak low-concentration signals in whole-tube detection. Step 6 The main control board (13) has a built-in data processing program that compares and fits the basic data of the whole tube in situ detection obtained in step 3 with the high-precision microdroplet detection data obtained in step 5, and combines them with the preset standard protein curve to automatically correct the detection error and finally calculate the precise micro protein concentration of the sample to be tested. After step 7 is completed, open the sealing cap (10) and take out the test tube. The sample can be retained for retesting if there is no large loss, thus completing the single micro-protein quantitative detection process.

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