A collection and detection integrated kit

Abstract

The application discloses a kind of collection detection integrated kit, it is related to medical detection technical field, a kind of collection detection integrated kit, including horizontal centrifugal separator, the protective shell for arranging horizontal centrifugal separator, protective base, the top of horizontal centrifugal separator is provided with sample storage rack, a plurality of sample tube protection devices are slidably connected on sample storage rack, sample tube protection device is used to store sample storage tube, sample storage tube, sample tube protection device work together in horizontal centrifugal separator, sample storage tube includes sample tube, the top of sample tube is sealingly connected with sealing terminal, sealing terminal is located at the position of sample tube inner wall and is provided with fixed block, the bottom of fixed block is provided with movable block, there is adhesion between fixed block and movable block, fixed block, movable block are fixedly connected with the filter screen one of folding setting, the filter screen two, the application can automatically centrifugal and filter synchronization separation to sample, improve work efficiency.

Description

Technical Field

[0001] This invention relates to the field of medical testing technology, specifically to an integrated reagent kit for collection and testing. Background Technology

[0002] Traditional blood testing procedures typically involve multiple steps, including blood collection, centrifugation, capping and transfer, filtration, and analysis. This process is not only inefficient but also prone to errors or contamination due to human intervention. It poses significant biosafety risks, especially when handling highly infectious samples. Existing technologies attempt to simplify the process through integrated design, such as using automated centrifuges to increase throughput. Therefore, there is an urgent need to develop a low-cost, fully enclosed, and multi-sample-type integrated reagent kit to integrate the entire chain of sample processing and analysis, reduce manual operations, and ensure the reliability of results.

[0003] Patent CN221980740U discloses an integrated blood collection and nucleic acid separation device, including a blood collection tube, a collection tube for collecting waste liquid, and a centrifuge tube for extracting nucleic acid. The blood collection tube is open at both ends, and the lower end of the blood collection tube is detachably connected and fixed to the collection tube or centrifuge tube as needed. An ultrafiltration membrane for filtering plasma serum and retaining blood cells is provided in the blood collection tube along the liquid flow direction, and a silicone membrane located behind the ultrafiltration membrane for filtering lysed blood cells and retaining nucleic acid from the blood cells. This integrated blood collection and nucleic acid separation device, by setting a detachable collection tube and centrifuge tube at the bottom of the blood collection tube and constructing a filter membrane assembly in the blood collection tube, allows for nucleic acid extraction simultaneously with blood collection, eliminating the need for sealing and waiting. This integration of blood collection and nucleic acid extraction functions reduces transfer steps and improves the efficiency of nucleic acid detection. The overall structure is simple and practical, and the production cost is low. However, this patent also has limitations in terms of the inconvenience of automating the processing and preservation of collected samples, and there is a risk of environmental contamination of the samples. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides an integrated acquisition and detection kit, which solves the problems mentioned in the background section.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solution: an integrated collection and detection kit, comprising a horizontal centrifuge, a centrifuge protective shell for housing the horizontal centrifuge, and a protective base. A sample storage rack is provided on the top of the horizontal centrifuge, and multiple sample tube protection devices are slidably connected to the sample storage rack. The sample tube protection devices are used to store sample storage tubes, and the sample storage tubes and sample tube protection devices work together inside the horizontal centrifuge. The sample storage tube includes a sample tube, the top of which is sealed with a sealing terminal, a fixed block is provided at the position of the sealing terminal on the inner wall of the sample tube, a movable block is provided at the bottom of the fixed block, there is an adhesive part between the fixed block and the movable block, and a folded filter screen one and a filter screen two are fixedly connected inside the fixed block and the movable block. The horizontal centrifuge breaks and separates the adhesion between the stationary and moving blocks under centrifugal force, allowing filter screens one and two to unfold for serum filtration and separation.

[0006] Furthermore, there are gaps between the stationary block, the moving block, and the inner wall of the sample tube to facilitate the filtration and permeation of serum.

[0007] Furthermore, a counterweight can be added to the bottom side of the moving block to facilitate the breakage and separation of the adhered parts when the rotational speed reaches a certain threshold.

[0008] Furthermore, low-speed centrifugation can be performed first, followed by high-speed centrifugation. The adhered parts break during rapid rotation, thus preventing the adhesion of large molecules to the surfaces of filter screens one and two, which would affect the filtration efficiency of the serum.

[0009] Furthermore, the top of the sealing terminal is a sealing rubber pad. When blood is introduced into the sample tube, it is inserted into the sample tube through the middle of the sealing rubber pad. When it is necessary to separate and extract serum, it is inserted through the outer part of the middle of the sealing rubber pad to achieve the purpose of extracting serum.

[0010] According to the above technical solution, the sample storage rack includes a frame, an auxiliary separation device is provided in the middle of the frame, an even number of placement holes are provided on the frame, and the placement holes are evenly distributed in pairs opposite each other. A guide gap is provided in the middle of the side of the placement hole near the auxiliary separation device. A sample tube protective device is slidably connected inside the placement hole.

[0011] Furthermore, the sample tube protection device is used to protect the sample storage tubes, preventing them from breaking due to vibration during centrifugation. In the event of an accident, the sample tube protection device can also prevent separation splashes and the resulting cleaning problems.

[0012] Furthermore, the number of mounting holes is preferably an even multiple of the number of separations per cycle of the horizontal centrifuge.

[0013] According to the above technical solution, a limiting frame is hinged on the side of the mounting hole near the auxiliary separation device, a magnetic column is fixedly connected to the bottom of the side of the limiting frame near the auxiliary separation device, and a limiting block is fixedly connected to the side of the limiting frame away from the auxiliary separation device near the rotating shaft. The limiting block is inserted into and protrudes from the mounting hole. A torsion spring is provided at the hinge of the limiting frame.

[0014] Furthermore, the limiting bracket fits tightly against the surface of the frame under the action of the torsion spring.

[0015] Furthermore, the magnetic poles on the same side of the first magnetic column are the same, and the magnetic poles on adjacent surfaces of the first magnetic column and the second magnetic strip are the same.

[0016] According to the above technical solution, the sample tube protection device includes a metal protective shell, the middle of which is hollowed out and filled with thermally conductive silicone. The thermally conductive silicone is attached to the outer wall of the sample tube. A drag is fixedly connected to the middle of the metal protective shell near the auxiliary separation device. A wedge-shaped groove is opened in the drag. A limit block two is fixedly connected to the side of the metal protective shell near the auxiliary separation device. The second limiting block corresponds to the position of the first limiting block on the sample storage rack.

[0017] Furthermore, the horizontal height of the drag buckle is higher than the horizontal height of the second limiting block.

[0018] Furthermore, thermally conductive silicone has excellent thermal conductivity and shock absorption. When the sample storage tube is inserted, the thermally conductive silicone will be squeezed and contracted, which increases the adhesion to the sample tube surface and also provides shock absorption.

[0019] According to the above technical solution, the auxiliary separation device includes a stepper motor one, a stepper motor two, and a lead screw. The stepper motor one is fixedly connected to the top of the sample storage rack. The stepper motor one is used to drive the lead screw to rotate. A transmission slider is threaded onto the stepper motor one. A pair of equidistant elastic slides are fixedly connected to the transmission slider. The elastic slides are U-shaped. One end of the elastic slide is fixedly connected to the transmission slider. The other end of the elastic slide is bent outward and has a forked part. A deflection part is provided on the side of the elastic slide away from the transmission slider. The top of the transmission slider is provided with a gear set one, and the stepper motor two is connected to the transmission slider through the gear set one.

[0020] Furthermore, a V-shaped guide surface exists at the end of the deflection section.

[0021] Furthermore, the adjacent U-shaped surfaces of the elastic slide can be filled with supporting ribs to improve the overall support performance of the elastic slide.

[0022] According to the above technical solution, multiple pairs of evenly distributed magnetic strips are fixedly connected to the outer side of the transmission slider, and magnetic strips are fixedly connected to the sample storage rack at the position corresponding to the magnetic strips, and the magnetic strips attract each other.

[0023] An electromagnet is fixedly connected to the transmission slider at the position corresponding to the elastic slide plate, and the electromagnet corresponds to the position of the magnetic column on the sample storage rack.

[0024] Furthermore, the two ends of the electromagnet have the same magnetic poles when it is in operation.

[0025] According to the above technical solution, the horizontal centrifuge includes a motor three, a gear set two, and a centrifuge shaft. The motor three is connected to the centrifuge shaft through the gear set two. A centrifuge frame is fixedly connected to the top of the centrifuge shaft. The centrifuge frame is hinged to both ends of the centrifuge frame. The mounting sleeves are used to fix the sample tube protection device. A deflection protrusion is fixedly connected to the side of the mounting sleeve near the centrifugal shaft. The position of the deflection protrusion corresponds to the hinge axis between the mounting sleeve and the centrifugal frame, and the position of the deflection protrusion corresponds to the deflection part on the auxiliary separation device.

[0026] According to the above technical solution, a positioning slot is provided at the bottom of the centrifugal shaft, and an electromagnetic lock is fixedly connected inside the centrifuge protective shell at the position corresponding to the positioning slot. The electromagnetic lock and the positioning slot are mutually compatible. The bottom of the centrifugal shaft is fixedly connected to a positioning slot.

[0027] According to the above technical solution, the temperature control device includes a heat sink, a semiconductor refrigeration chip, and a thermally conductive phase change material. The cooling end of the semiconductor refrigeration chip transfers heat to the thermally conductive silicone inside the metal protective shell through the thermally conductive phase change material. The heating end of the semiconductor refrigeration chip is thermally connected to the heat sink. The bottom of the protective base has a ventilation hole one, the bottom of the centrifuge protective shell has a ventilation hole two, and the sample storage rack has a ventilation hole three at the position corresponding to the temperature control device.

[0028] This invention provides an integrated reagent kit for data collection and detection. It has the following beneficial effects: This invention integrates filter one and filter two onto a sealed terminal. During blood centrifugation, the moving block unfolds under centrifugal force. The automatically unfolding double-layered filter one and filter two directly intercept impurities such as blood cells and fibrin. Serum permeates through the filter into the sealed space formed by filter one and filter two, eliminating the need for manual transfer and filtration after centrifugation, reducing operation time and the risk of cross-contamination. It is particularly suitable for batch sample processing. At the same time, the adhesive design of the fixed and moving blocks maintains a seal before centrifugation, preventing sample leakage during transportation or pretreatment. After separation, the serum is directly stored in the space between filter one and filter two without opening the cap for transfer, reducing the risk of environmental or operator contact. This invention enables the directional transport of materials in a horizontal centrifuge by using an auxiliary separation device to drive the sample tube protection device to slide up and down. At the same time, it can reposition the separated materials in the sample storage rack for low-temperature storage. The automatic loading and unloading of sample storage tubes is achieved through the auxiliary separation device, eliminating the need for manual operation throughout the process and significantly shortening the sample processing time. When processing hundreds of samples in batches, traditional centrifuges require placing each tube individually, and the long separation time greatly affects the efficiency of blood separation.

[0029] This invention improves the protection of the sample storage tube and enhances its temperature control by adding a sample tube protection device to the outside of the sample storage tube. The matching of a single set of thermally conductive phase change material with a single sample tube protection device better achieves temperature control of the sample tube protection device. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the overall half-section structure of the present invention; Figure 2 This is a schematic diagram of the overall structure of the present invention; Figure 3 This is a schematic diagram of the overall sample storage tube of the present invention; Figure 4 This invention as a whole Figure 3 A schematic diagram of a half-section structure; Figure 5 This is a schematic diagram of the overall sample storage rack and adjacent structures of the present invention; Figure 6 This invention as a whole Figure 5 A schematic diagram of the bottom side structure; Figure 7 This invention as a whole Figure 6 A schematic diagram of the structure of area A; Figure 8 This is a schematic diagram of the overall limiting frame of the present invention; Figure 9 This is a schematic diagram of the overall sample tube protection device and temperature control device of the present invention; Figure 10 This invention as a whole Figure 5 Schematic diagram of half-section structure; Figure 11 This is a schematic diagram of the overall auxiliary separation device of the present invention; Figure 12 This is a schematic diagram of the overall horizontal centrifugal separator of the present invention; Figure 13 This invention as a whole Figure 12 A structural diagram of area B.

[0031] In the diagram: 1. Sample storage rack; 101. Frame; 102. Mounting hole; 103. Guide gap; 104. Magnetic strip one; 106. Limiting frame; 107. Magnetic column one; 108. Limiting block one; 2. Sample storage tube; 201. Sample tube; 202. Sealing terminal; 203. Fixed block; 204. Moving block; 205. Filter screen one; 206. Filter screen two; 3. Sample tube protection device; 301. Metal protective shell; 302. Thermally conductive silicone; 303. Clip; 304. Limiting block two; 4. Temperature control device; 401. Heat sink; 402. Semiconductor refrigeration chip; 403. Thermally conductive phase change material; 5. 501. Auxiliary separation device; 502. Stepper motor 1; 503. Stepper motor 2; 504. Transmission slider; 505. Lead screw; 506. Magnetic strip 2; 507. Electromagnet; 508. Elastic slide plate; 509. Forking part; 510. Deflection part; 6. Horizontal centrifuge; 601. Motor 3; 602. Gear set 2; 603. Centrifuge shaft; 604. Centrifuge frame; 605. Mounting sleeve; 606. Deflection protrusion; 607. Positioning slot; 7. Electromagnetic lock; 8. Ventilation hole 1; 9. Ventilation hole 2; 10. Ventilation hole 3; 11. Centrifuge protective shell; 12. Protective base. Detailed Implementation

[0032] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0033] Please see Figure 1-13 A sample collection and detection integrated kit includes a horizontal centrifuge 6, a centrifuge protective shell 11 for housing the horizontal centrifuge 6, and a protective base 12. A sample storage rack 1 is provided on the top of the horizontal centrifuge 6. Multiple sample tube protection devices 3 are slidably connected on the sample storage rack 1. The sample tube protection devices 3 are used to store sample storage tubes 2. The sample storage tubes 2 and the sample tube protection devices 3 work together inside the horizontal centrifuge 6. The sample storage tube 2 includes a sample tube 201. A sealing terminal 202, which is a rubber stopper, is sealed to the top of the sample tube 201. The sealing terminal 202 is inserted into the port of the sample tube 201 to form a sealed connection. Both can be secured to the outside of their connection point using an external retainer, further limiting the connection between the sealing terminal 202 and the sample tube 201. A fixing block 203 is located on the inner wall of the sample tube 201 at the position of the sealing terminal 202. The fixing block 203 is annular in shape and has an annular groove on the side facing the inner wall of the sample tube 201. The fixing block 203 is fixed to the sealing terminal 201. The sealing terminal 202 faces the inner wall of the sample tube 201. A movable block 204 is provided at the bottom of the fixed block 203. There is an adhesive part between the fixed block 203 and the movable block 204. A folded filter screen 1 205 and filter screen 206 are fixedly connected inside the fixed block 203 and the movable block 204. The filter screen 1 205 and filter screen 206 are cylindrical in the unfolded state, and the filter screen 206 is located inside the filter screen 1 205. When the fixed block 203 and the movable block 204 are connected at the adhesive part, they are in a wave-folded state. The upper and lower ends of the filter screen 1 205 and filter screen 206 are fixedly connected to the fixed block 203 and the movable block 204 in an arc. The horizontal centrifuge 6 breaks and separates the adhesive parts between the stationary block 203 and the moving block 204 under the action of centrifugal force, so that the filter screen 1 205 and the filter screen 206 are unfolded to filter and separate the serum.

[0034] Furthermore, there is a gap between the fixed block 203, the moving block 204 and the inner wall of the sample tube 201 to facilitate the filtration and permeation of serum.

[0035] Furthermore, the fixed block 203 and the moving block 204 are symmetrically arranged and have the same structure. A counterweight can be added to the bottom side of the moving block 204 so that the adhesive part can break and separate when the rotation speed reaches a certain threshold.

[0036] Furthermore, low-speed centrifugation can be performed first, followed by high-speed centrifugation. The adhered parts break during rapid rotation, thus preventing the adhesion of large molecules on the surfaces of filter screen 205 and filter screen 206, which would affect the filtration efficiency of the serum.

[0037] Furthermore, the top of the sealing terminal 202 is a sealing rubber pad. When blood is introduced into the sample tube 201, it is inserted into the sample tube 201 through the middle of the sealing rubber pad. When it is necessary to separate and extract serum, it is inserted through the outer side of the middle of the sealing rubber pad to achieve the purpose of extracting serum.

[0038] According to the above structure, blood is first collected through sample tube 201, and then separated by the horizontal centrifuge 6. When the rotational speed of the horizontal centrifuge 6 exceeds the breakage threshold of the adhesion part, the stationary block 203 separates from the moving block 204, and the first filter screen 205 and the second filter screen 206 are unfolded. At this time, the moving block 204 extends into the separated blood, and the serum in the blood seeps into the space formed by the first filter screen 205 and the second filter screen 206 through filtration. After separation, the serum accumulates in the upper part of sample tube 201, wetting filter screens 205 and 206. Over time, the space formed by filter screens 205 and 206 will be filled with serum. When serum needs to be collected for testing, medical personnel insert a syringe into the outside of the sealing rubber pad and insert the needle into the space formed by filter screens 205 and 206. At this time, medical personnel can collect serum containing fewer impurities without observing the test tube, thus ensuring the accuracy of the test.

[0039] The sample storage rack 1 includes a frame 101, an auxiliary separation device 5 is provided in the middle of the frame 101, an even number of placement holes 102 are provided on the frame 101, and the placement holes 102 are evenly distributed in pairs opposite each other. A guide gap 103 is provided in the middle of the side of the placement hole 102 closest to the auxiliary separation device 5. A sample tube protective device 3 is slidably connected inside the placement hole 102.

[0040] Furthermore, the sample tube protection device 3 is used to protect the sample storage tube 2 to prevent the sample storage tube 2 from breaking due to vibration during centrifugation. At the same time, even if an accident occurs, the sample tube protection device 3 can also prevent separation splashes and cleaning problems.

[0041] Furthermore, the number of mounting holes 102 is preferably an even multiple of the number of separations per cycle of the horizontal centrifugal separator 6.

[0042] A limiting frame 106 is hinged to the side of the mounting hole 102 near the auxiliary separation device 5. A magnetic column 107 is fixedly connected to the bottom of the side of the limiting frame 106 near the auxiliary separation device 5. A limiting block 108 is fixedly connected to the side of the limiting frame 106 away from the auxiliary separation device 5 near the rotating shaft. The limiting block 108 is inserted into and protrudes from the mounting hole 102. A torsion spring is provided at the hinge of the limit bracket 106.

[0043] Furthermore, the limiting bracket 106 is in close contact with the surface of the frame 101 under the action of the torsion spring.

[0044] Furthermore, the magnetic poles on the same side of the magnet column 107 are the same, and the magnetic poles on adjacent surfaces of the magnet column 107 and the magnetic strip 505 are the same.

[0045] According to the above structure, the limiting frame 106 is tightly attached to the surface of the frame 101 under the action of the torsion spring, so that the limiting block 108 is kept filled in the inner wall of the insertion hole 102, thereby limiting the limiting block 304 on the sample tube protection device 3.

[0046] The sample tube protection device 3 includes a metal protective shell 301, the middle of which is hollowed out and filled with thermally conductive silicone 302. The thermally conductive silicone 302 is attached to the outer wall of the sample tube 201. A drag buckle 303 is fixedly connected to the middle of the side of the metal protective shell 301 near the auxiliary separation device 5. A wedge-shaped groove is opened in the drag buckle 303. A limit block 2 304 is fixedly connected to the side of the metal protective shell 301 near the auxiliary separation device 5. The position of limit block 2 304 corresponds to that of limit block 1 108 on sample storage rack 1.

[0047] Furthermore, the horizontal height of the drag bar 303 is higher than the horizontal height of the limit block 304.

[0048] Furthermore, the thermally conductive silicone 302 has good thermal conductivity and shock absorption. When the sample storage tube 2 is inserted, the thermally conductive silicone 302 will be squeezed and contracted, which increases the adhesion to the surface of the sample tube 201 and the shock absorption effect.

[0049] According to the above structure, by dragging the drag buckle 303, the second limiting block 304 is supported by the first limiting block 108, thereby fixing the position of the sample tube protection device 3.

[0050] The auxiliary separation device 5 includes a first stepper motor 501, a second stepper motor 502, and a lead screw 504. The first stepper motor 501 is fixedly connected to the top of the sample storage rack 1. The first stepper motor 501 is used to drive the lead screw 504 to rotate. A transmission slider 503 is threadedly connected to the first stepper motor 501. A pair of equidistant elastic slide plates 507 are fixedly connected to the transmission slider 503. The elastic slide plates 507 are U-shaped. One end of the elastic slide plate 507 is fixedly connected to the transmission slider 503. The other end of the elastic slide plate 507 is bent outward to have a forked part 508. A deflection part 509 is provided on the side of the elastic slide plate 507 away from the transmission slider 503. The top of the transmission slider 503 is provided with a gear set 510, and the stepper motor 502 is connected to the transmission slider 503 through the gear set 510.

[0051] Furthermore, the deflection section 509 has a V-shaped guide surface at its end.

[0052] Furthermore, the adjacent U-shaped surfaces of the elastic slide 507 can be filled with support ribs to improve the overall support performance of the elastic slide 507.

[0053] According to the above structure, the stepper motor 501 drives the lead screw 504 to rotate, controlling the transmission slider 503 and the elastic slide plate 507 fixed thereto to move up and down synchronously. When the forked part 508 is engaged in the drag buckle 303, the entire sample tube protection device 3 can be moved along with the upward movement of the transmission slider 503 until the limit block 304 and the limit block 108 are engaged with each other, thereby placing the separated sample storage tube 2 back into the sample storage rack 1.

[0054] Multiple pairs of evenly distributed magnetic strips 505 are fixedly connected to the outer side of the transmission slider 503. Magnetic strip 104 is fixedly connected to the sample storage rack 1 at the position corresponding to magnetic strip 505. Magnetic strip 505 and magnetic strip 104 attract each other.

[0055] According to the above structure, since the transmission slider 503 may rotate synchronously with the rotation of the lead screw 504 when the lead screw 504 rotates, the transmission slider 503 is pulled outward in multiple directions by the mutual attraction between the multiple magnetic strips 104 and the magnetic strip 505, so that the transmission slider 503 can slide up or down under the operation of the stepper motor 501.

[0056] An electromagnet 506 is fixedly connected to the transmission slider 503 at the position corresponding to the elastic slide plate 507. The electromagnet 506 corresponds to the position of the magnet column 107 on the sample storage rack 1.

[0057] Furthermore, the two ends of the electromagnet 506 have the same magnetic poles when it is in operation.

[0058] According to the above structure, when the sample tube protection device 3 needs to be transported to the horizontal centrifuge 6, the stepper motor 501 controls the motor drive slider 503 to move to the highest point. At this time, the position of the magnetic column 107 corresponds to that of the electromagnet 506, and the elastic slide 507 is inserted into the drag buckle 303. At this time, the electromagnet 506 is powered. At this time, the magnetic poles of the adjacent surfaces of the magnetic column 107 and the electromagnet 506 are different, and they attract each other. Therefore, the limiting frame 106 flips under the magnetic force of the magnetic column 107, so that the limiting block 108 disengages from the limiting block 304. At this time, the control screw 504 rotates in the opposite direction to transport the sample tube protection device 3 into the horizontal centrifuge 6.

[0059] The horizontal centrifuge 6 includes a motor 601, a gear set 602, and a centrifuge shaft 603. The motor 601 is connected to the centrifuge shaft 603 through the gear set 602. A centrifuge frame 604 is fixedly connected to the top of the centrifuge shaft 603. The centrifuge frame 604 is hinged to both ends of the centrifuge frame 604 and the mounting sleeve 605 is used to fix the sample tube protection device 3. A deflection protrusion 606 is fixedly connected to the side of the mounting sleeve 605 near the centrifugal shaft 603. The position of the deflection protrusion 606 corresponds to the hinge axis between the mounting sleeve 605 and the centrifugal frame 604, and the position of the deflection protrusion 606 corresponds to the position of the deflection part 509 on the auxiliary separation device 5.

[0060] According to the above structure, when the metal protective shell 301 moves downward and is inserted into the mounting sleeve 605, as the metal protective shell 301 moves downward, the limiting block 304 contacts the edge of the mounting sleeve 605. At this time, the transmission slider 503 continues to move downward. At this time, the elastic slide plate 507 disengages from the drag buckle 303 and, supported by the mounting sleeve 605, adheres to the surface of the mounting sleeve 605. When the V-shaped guide surface of the deflection part 509 contacts the deflection protrusion 606, the deflection protrusion 606 is subjected to a tangential force, which causes the centrifuge rack 604 to deflect at a small angle. At this time, it moves on the control positioning slot 607, thereby achieving the purpose of conveying the sample tube protection device 3 to the deflection protrusion 606 on the horizontal centrifuge 6.

[0061] The bottom of the centrifugal shaft 603 is provided with a positioning slot 607. An electromagnetic lock 7 is fixedly connected inside the centrifuge protective shell 11 at the position corresponding to the positioning slot 607. The electromagnetic lock 7 and the positioning slot 607 are mutually compatible. The bottom of the centrifugal shaft 603 is fixedly connected to a positioning slot 607.

[0062] According to the above structure, after the single sample storage tube 2 is separated, the motor 601 starts to decelerate. When the speed is reduced to a certain threshold, the electromagnetic lock 7 is controlled to work until the working part of the electromagnetic lock 7 is engaged in the positioning slot 607, and the centrifugal shaft 603 stops rotating. This ensures that the position of the centrifugal shaft 603 remains unchanged when the machine stops, which facilitates the loading and unloading of the sample tube protection device 3 on the horizontal centrifuge 6.

[0063] The temperature control device 4 includes a heat sink 401, a semiconductor refrigeration chip 402, and a thermally conductive phase change material 403. The cooling end of the semiconductor refrigeration chip 402 is heat-transferred to the thermally conductive silicone 302 inside the metal protective shell 301 through the thermally conductive phase change material 403. The heating end of the semiconductor refrigeration chip 402 is thermally connected to the heat sink 401. The bottom of the protective base 12 has a ventilation hole 8, the bottom of the centrifuge protective shell 11 has a ventilation hole 9, and the sample storage rack 1 has a ventilation hole 10 at the position corresponding to the temperature control device 4.

[0064] Furthermore, the heat sink 401, the semiconductor cooling chip 402, the thermally conductive phase change material 403, and the metal protective shell 301 are closely fitted together on their adjacent surfaces, with the heat dissipation surface of the heat sink 401 facing the overall shell of the device and in contact with the outside air.

[0065] According to the above structure, the rotation of the centrifugal shaft 603 synchronously drives the mounting sleeve 605 to rotate, and transports the outside air from the ventilation hole 1 8 and ventilation hole 2 9 to the outlet of the ventilation hole 3 10, and realizes the rapid transport of the heat generated by the semiconductor cooling chip 402 when it is working, so as to ensure the cooling effect of the semiconductor cooling chip 402. Meanwhile, the thermally conductive phase change material 403 has good thermal conductivity and wear resistance. Combined with the metal shell of the metal protective shell 301 and the filling material of the thermally conductive silicone 302, it ensures that the cold source of the semiconductor cooling chip 402 can continuously preserve the sample storage tube 2 at low temperature, thereby ensuring the freshness of the blood after separation.

[0066] Working principle: Blood is first collected through sample tube 201, and then separated by horizontal centrifuge 6. When the rotational speed of horizontal centrifuge 6 exceeds the breakage threshold of the adhesion part, the stationary block 203 separates from the moving block 204, and the first filter screen 205 and the second filter screen 206 unfold. At this time, the moving block 204 extends into the separated blood. The serum in the blood seeps into the space formed by the first filter screen 205 and the second filter screen 206 through filtration. After separation, the serum accumulates in the upper part of sample tube 201, wetting filter screen 205 and filter screen 206. As time goes on, the space formed by filter screen 205 and filter screen 206 will be filled with serum. When it is necessary to collect serum for testing, medical personnel insert a syringe into the outside of the sealing rubber pad and insert the needle into the space formed by filter screen 205 and filter screen 206. At this time, medical personnel can collect serum containing fewer impurities without observing the test tube, thus ensuring the accuracy of the test. Simultaneously, the auxiliary separation device 5 drives the sample tube protection device 3 to slide up and down, realizing the directional delivery of materials to the horizontal centrifuge 6. At the same time, the separated materials can be repositioned in the sample storage rack 1 for low-temperature storage. Automatic loading and unloading of sample storage tubes 2 is achieved through the auxiliary separation device 5. No manual operation is required throughout the process, which greatly shortens the sample processing time and provides a faster sample processing capability when hundreds of samples need to be processed in batches.

[0067] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A kit for integrated collection and detection, comprising a horizontal centrifuge (6), a centrifuge protective shell (11) for housing the horizontal centrifuge (6), and a protective base (12), characterized in that: The top of the horizontal centrifuge (6) is provided with a sample storage rack (1), and multiple sample tube protection devices (3) are slidably connected on the sample storage rack (1). The sample tube protection devices (3) are used to store sample storage tubes (2). The sample storage tubes (2) and the sample tube protection devices (3) work together in the horizontal centrifuge (6). The sample storage tube (2) includes a sample tube (201). A sealing terminal (202) is sealed to the top of the sample tube (201). A fixed block (203) is provided at the position of the sealing terminal (202) on the inner wall of the sample tube (201). A movable block (204) is provided at the bottom of the fixed block (203). There is an adhesive part between the fixed block (203) and the movable block (204). A folded filter screen one (205) and a filter screen two (206) are fixedly connected inside the fixed block (203) and the movable block (204). By operating the horizontal centrifuge (6), the adhesion between the fixed block (203) and the moving block (204) is broken and separated under the action of centrifugal force, so that filter screen one (205) and filter screen two (206) are unfolded to filter and separate the serum.

2. The integrated acquisition and detection reagent kit according to claim 1, characterized in that: The sample storage rack (1) includes a frame (101), an auxiliary separation device (5) is provided in the middle of the frame (101), an even number of placement holes (102) are provided on the frame (101), and the placement holes (102) are evenly distributed in pairs opposite each other. A guide gap (103) is provided in the middle of the side of the placement hole (102) near the auxiliary separation device (5). A sample tube protective device (3) is slidably connected inside the placement hole (102).

3. The integrated acquisition and detection reagent kit according to claim 2, characterized in that: A limiting frame (106) is hinged to the side of the mounting hole (102) near the auxiliary separation device (5). A magnet column (107) is fixedly connected to the bottom of the side of the limiting frame (106) near the auxiliary separation device (5). A limiting block (108) is fixedly connected to the side of the limiting frame (106) away from the auxiliary separation device (5) near the rotating shaft. The limiting block (108) is inserted into and protrudes from the mounting hole (102). A torsion spring is provided at the hinge of the limiting frame (106).

4. The integrated acquisition and detection reagent kit according to claim 2, characterized in that: The sample tube protection device (3) includes a metal protective shell (301), the middle of which is hollowed out and filled with thermally conductive silicone (302). The thermally conductive silicone (302) is attached to the outer wall of the sample tube (201). A drag buckle (303) is fixedly connected to the middle of the side of the metal protective shell (301) near the auxiliary separation device (5). A wedge-shaped groove is provided in the drag buckle (303). A limit block two (304) is fixedly connected to the side of the metal protective shell (301) near the auxiliary separation device (5). The second limiting block (304) corresponds to the position of the first limiting block (108) on the sample storage rack (1).

5. The integrated acquisition and detection reagent kit according to claim 2, characterized in that: The auxiliary separation device (5) includes a stepper motor (501), a stepper motor (502), and a lead screw (504). The stepper motor (501) is fixedly connected to the top of the sample storage rack (1). The stepper motor (501) is used to drive the lead screw (504) to rotate. A transmission slider (503) is threaded onto the stepper motor (501). A pair of equidistant elastic slides (507) are fixedly connected to the transmission slider (503). The elastic slides (507) are U-shaped. One end of the elastic slides (507) is fixedly connected to the transmission slider (503). The other end of the elastic slides (507) is bent outward and has a forked part (508). A deflection part (509) is provided on the side of the elastic slides (507) away from the transmission slider (503). The top of the transmission slider (503) is provided with a gear set (510), and the stepper motor (502) is connected to the transmission slider (503) through the gear set (510).

6. The integrated acquisition and detection reagent kit according to claim 5, characterized in that: The outer side of the transmission slider (503) is fixedly connected with multiple pairs of evenly distributed magnetic strips two (505), and the sample storage rack (1) is fixedly connected with magnetic strip one (104) at the position corresponding to magnetic strip two (505). Magnetic strip two (505) and magnetic strip one (104) attract each other.

7. The integrated acquisition and detection reagent kit according to claim 5, characterized in that: An electromagnet (506) is fixedly connected to the transmission slider (503) at the position corresponding to the elastic slide plate (507), and the electromagnet (506) corresponds to the position of the magnet post (107) on the sample storage rack (1).

8. The integrated acquisition and detection reagent kit according to claim 7, characterized in that: The horizontal centrifuge (6) includes a motor (601), a gear set (602), and a centrifuge shaft (603). The motor (601) is connected to the centrifuge shaft (603) via the gear set (602). A centrifuge frame (604) is fixedly connected to the top of the centrifuge shaft (603). A mounting sleeve (605) is hinged to both ends of the centrifuge frame (604). The mounting sleeve (605) is used to fix the sample tube protection device (3). A deflection protrusion (606) is fixedly connected to the side of the mounting sleeve (605) near the centrifugal shaft (603). The position of the deflection protrusion (606) corresponds to the hinge axis between the mounting sleeve (605) and the centrifugal frame (604). The position of the deflection protrusion (606) corresponds to the deflection part (509) on the auxiliary separation device (5).

9. The integrated acquisition and detection reagent kit according to claim 8, characterized in that: The bottom of the centrifugal shaft (603) is provided with a positioning slot (607), and an electromagnetic latch (7) is fixedly connected inside the centrifuge protective shell (11) at the position corresponding to the positioning slot (607). The electromagnetic latch (7) and the positioning slot (607) are mutually compatible. The bottom of the centrifugal shaft (603) is fixedly connected to a positioning slot (607).

10. The integrated acquisition and detection reagent kit according to claim 9, characterized in that: The temperature control device (4) includes a heat sink (401), a semiconductor refrigeration chip (402), and a thermally conductive phase change material (403). The cooling end of the semiconductor refrigeration chip (402) transfers heat to the thermally conductive silicone (302) inside the metal protective shell (301) through the thermally conductive phase change material (403). The heating end of the semiconductor refrigeration chip (402) is thermally connected to the heat sink (401). The bottom of the protective base (12) is provided with a ventilation hole 1 (8), the bottom of the centrifuge protective shell (11) is provided with a ventilation hole 2 (9), and the sample storage rack (1) is provided with a ventilation hole 3 (10) at the position corresponding to the temperature control device (4).

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