A multi-light-source image fusion dark field imaging device for biological sample containers

Abstract

The application discloses a biological sample container multi-light-source image fusion dark field imaging device. The device is compactly arranged with multiple small second light sources, and realizes high uniformity of a background of dark field imaging of a culture dish without a large annular light source, thereby greatly saving space and power consumption. The device retains key surface information such as color and texture of a bacterial colony, and has high image contrast, thereby greatly facilitating subsequent visual algorithm development such as automatic bacterial colony counting and morphology analysis. The hardware structure is flexible, and supports multiple layouts such as a same side, a different side and a mirror, thereby adapting to requirements of different integrated environments. The fusion algorithm is diverse and configurable, and can select a minimum value fusion and a weighted average strategy according to actual imaging effects, and can be combined with a back light source bright field image to optimize imaging results. The device realizes same-frame imaging of a sample and a mark through an annular second mirror, and improves efficiency and accuracy of automatic data management.

Description

Technical Field

[0001] This invention relates to a dark-field imaging device for multi-source image fusion of biological sample containers. Background Technology

[0002] In life science research such as microbial culture and cell biology, as well as clinical testing, petri dishes are among the most commonly used tools. To automate the analysis of culture results (such as colony counting and morphological analysis), high-quality digital images are typically required. Existing imaging illumination technologies are mainly divided into two types, both of which have significant limitations.

[0003] The first method is bottom-transmitted light source illumination. This method can obtain bright-field images with uniform background and high contrast, but it completely loses surface details such as colony color and texture, and cannot meet the analytical needs of identifying colony species or observing fine morphology.

[0004] The second method is transmissive ring-shaped backlight illumination. This method utilizes the scattering of light by the colonies for imaging, which can better preserve the colony color and texture, achieving dark-field imaging. However, due to the relatively large diameter of the culture dish (typically 90mm), when the size of the ring light source is limited (e.g., diameter less than 200mm), the distance between the edge of the culture dish and the light source is much greater than the distance to the center. According to the physical law that "illuminance decreases approximately inversely with distance," this leads to severe uneven illumination on the sample surface, with the central area underexposed and the edges overexposed. To achieve uniform illumination, current technology is forced to use a large ring light source with a diameter much larger than the culture dish, resulting in significant waste of space and materials. Furthermore, to obtain sufficient illumination at a greater distance, high power consumption is required, which contradicts the current trend of miniaturization and low power consumption in instrumentation. Areas close to the light source are significantly illuminated, while the entire background is overexposed, and the brightness gradient is also very obvious the closer to the light source.

[0005] Such brightness gradients are detrimental to image analysis. Small spikes can be roughly considered as signals of bacterial colonies, and extracting these signals (e.g., A) is relatively easy when the overall background illumination is low and relatively uniform. However, extracting signals (e.g., B) is more difficult when the background itself varies significantly.

[0006] Therefore, there are two ways to obtain an image with a low gradient: 1. Increase the distance between the light source and the subject, which is the traditional approach, i.e., using a large ring light source; 2. Abandon the area close to the light source, use multiple light sources, and take the relatively uniform part that is far away from the light source in the image of each light source.

[0007] Therefore, there is an urgent need in this field for a dark-field imaging technology that can stably obtain a uniform background and preserve the color texture of bacterial colonies under the constraints of size and power consumption. Summary of the Invention

[0008] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a device that can obtain highly uniform agar dark-field images without the need for a large ambient light source. Through hardware layout and integration with existing software algorithms, a high-quality image with highly uniform background and extremely high contrast between dark and bright areas can be automatically synthesized, ensuring the accuracy and reliability of subsequent image analysis, as well as the aesthetic appeal of the image.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: a multi-source image fusion dark-field imaging device for biological sample containers, comprising a device body, a stage within the device body, and a camera disposed at the top of the device body or at the bottom of the stage within the device body for capturing images of the stage area. As an improvement, the device body comprises at least two second light sources, each second light source illuminating the lid of the biological sample container on the stage area at a different orientation. The device also includes a controller for controlling the second light sources to illuminate in turn according to a preset sequence and time interval, and controlling the camera to perform exposure and capture when the corresponding second light source is illuminated. When the camera is at least located at the bottom of the stage and / or the second light source is located at the bottom of the stage, the stage is a light-transmitting stage. The second light sources and the camera are located on the same side and / or opposite sides of the stage. The camera captures images along the outermost edge of the biological sample container. The area inside the normal passing through the outermost edge of the biological sample container is a first region, and the area outside the normal is a second region. The second light source is located within the second region. By adopting the above technical solution: by arranging multiple second light sources that illuminate from different directions, and only lighting one light source at a time for shooting, the scattered light information of the target can be obtained from multiple directions; then, through subsequent image fusion algorithms, the information of the uniformly illuminated area in each image (i.e. the area far away from the illuminated light source) is used to synthesize an image with uniform overall illumination and high contrast between dark and bright areas, thus replacing the traditional large ring light source in a compact space.

[0010] Preferably, the second region is the area covered by the largest-sized biological sample container.

[0011] Preferably, the system also includes at least one backlight source capable of covering the entire field of view for illuminating the back of the petri dish to obtain a bright-field image. By employing the above technical solution, the backlight source can be used to acquire a bright-field image of the bright field, which can serve as an independent information source for target analysis before the dark-field image is captured, or as an auxiliary information source for the synthesis of the dark-field image.

[0012] Preferably, the second light source and the camera are positioned on the same side of the stage, between the camera and the stage, with the emitting surface of the second light source tilted towards the lid of the biological sample container on the stage. By adopting the above technical solution: the second light source and the camera are positioned on the same side, which facilitates the construction of a compact imaging optical path and reduces the thickness of the device; the tilted illumination method effectively excites the scattering of light by the culture surface, forming a dark-field effect, while avoiding glare caused by the light source directly hitting the lens.

[0013] Preferably, the second light source and the camera are positioned on opposite sides of the stage, with the camera positioned above the stage and the second light source positioned below the stage. The emitting surface of the second light source is tilted towards the lid of the biological sample container on the stage. By adopting the above technical solution, the second light source illuminates from below at an angle, and the light passes through a semi-transparent culture medium base (such as agar) to illuminate the bottom of the colony before being scattered. This allows for the acquisition of richer bottom texture information, complementing the illumination method from above and enhancing the detail of the fused image.

[0014] Preferably, the second light source comprises at least three, arranged in a ring. By adopting the above technical solution, three or more ring-shaped second light sources can provide illumination from a more circumferential and uniform direction, ensuring that all areas of the subject have usable scattered signals at an appropriate distance from the second light source.

[0015] Preferably, the device also includes a first reflector that reflects the stage area to the camera, with the camera facing the first reflector. By adopting the above technical solution, the camera can be positioned to the side of the device rather than directly above it by using the first reflector to change the optical path, effectively reducing the overall height of the device. This allows the imaging system to be integrated into a flatter device, enhancing structural flexibility and spatial adaptability.

[0016] Preferably, the second light source is a surface light source, a line light source, and / or a point light source. By adopting the above technical solution: different types of light sources can adapt to different cost and effect requirements; surface light sources provide uniform illumination; line or point light sources are lower in cost and have stronger directionality; the light-transmitting stage facilitates the illumination of the light source below, providing a structural basis for realizing diverse lighting layouts (on the same side, opposite side).

[0017] Preferably, a ring-shaped second reflector is also included. This second reflector is positioned around the corresponding location on the stage, around the periphery of the biological sample container. The ring-shaped second reflector is configured to reflect the side markings of the biological sample container on the stage to the inner mirror surface of the camera, allowing the camera to simultaneously capture both the sample image (facing the camera) and the image of the markings on the side of the biological sample container. By employing this technical solution, the second reflector cleverly reflects the label information on the side of the culture dish into the camera's field of view, achieving simultaneous capture of the sample image and the identification label. This eliminates the need for an additional camera or moving the culture dish, significantly improving the efficiency and accuracy of automated identification and data association.

[0018] Therefore, the present invention has the following beneficial effects: (1) By using multiple compactly arranged small second light sources, the background height of dark-field imaging of petri dishes is made uniform without the need for a large ring light source, which significantly saves space and power consumption; (2) The photos taken by different light sources can retain key surface information such as colony color and texture, and the image contrast is high, which greatly facilitates the development of visual algorithms such as automatic colony counting and morphological analysis; (3) The hardware structure is flexible and supports multiple layouts such as same side, opposite side, and reflector, which can adapt to the needs of different integrated environments; (4) The same frame imaging of samples and labels is realized by the ring second reflector, which improves the efficiency and accuracy of automated data management. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of an embodiment of the present invention (same-side illumination).

[0020] Figure 2 For the present invention Figure 1 Front view of the device (with lighting on the same side) Figure 3 This is a schematic diagram of an embodiment of the present invention (different side lighting).

[0021] Figure 4 For the present invention Figure 3 Front view of the device (with lighting from the opposite side).

[0022] Figure 5 This is a schematic diagram of the opposite-side illumination and the second light source surrounding the perimeter of the present invention.

[0023] Figure 6 For the present invention Figure 5 The front view.

[0024] Figure 7 The present invention includes a schematic diagram of opposite-side illumination, a second light source surrounding the perimeter, and a backlight.

[0025] Figure 8 This is the present invention. Figure 7The front view.

[0026] Figure 9 This is a schematic diagram of a camera with a first reflecting mirror for illumination from the opposite side.

[0027] Figure 10 This is the present invention. Figure 9 The front view.

[0028] Figure 11 This is the present invention. Figure 9 Side view front view Figure 12-15 These are photos taken from a second light source at different locations.

[0029] Figure 16 yes Figure 12-15 The composite photo.

[0030] Figure 17 This is a schematic diagram of the second reflector of the present invention positioned below the imaging container platform.

[0031] Figure 18 yes Figure 17 The front view.

[0032] Figure 19 This is a schematic diagram of the second reflector of the present invention positioned above the imaging container.

[0033] Figure 20 yes Figure 19 The front view.

[0034] Figure 21 This is a schematic diagram showing the second reflector of the present invention being flush with the imaging container.

[0035] Figure 22 yes Figure 21 The front view.

[0036] Figure 23 This is a schematic diagram illustrating the principle of image fusion.

[0037] Figure 24-31 This is a schematic diagram of the structure where the camera is mounted at the bottom of the stage.

[0038] In the figure: device body 1, stage 3, camera 2, first reflector 4, biological sample container 5, second light source 6, backlight 7, light source diffuser 71, second reflector 8, inner mirror 81. Detailed Implementation

[0039] To make the technical problems to be solved, the technical solutions, and the beneficial technical effects of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and several exemplary embodiments. It should be understood that the specific embodiments described herein are only for explaining the present invention and are not intended to limit the scope of protection of the present invention. It should be understood that in this document, the expressions "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance, nor should they be construed as implicitly specifying the number of technical features indicated. Features defined with "first" or "second" may explicitly or implicitly indicate that at least one of those features is included.

[0040] like Figure 1-11 , Figure 24-28 The diagram illustrates a multi-source image fusion dark-field imaging device for biological sample containers. It includes a device body 1, a stage 3 for placing biological sample containers 5 within the body 1, a camera 2 positioned at the top of the body 1 or at the bottom of the stage 3 for capturing images of the stage 3 area, and at least two second light sources 6. Each second light source 6 illuminates a different location on the lid of the biological sample container placed on the stage 3 area. The device also includes a controller for controlling the second light sources 6 to illuminate sequentially and at preset time intervals, and for controlling the camera 2 to perform exposure and imaging when the corresponding second light source 6 is illuminated. The controller can be a microcontroller. Figure 2 As shown, the camera 2 captures images along the outermost edge of the biological sample container 5. The area inside (upper) of the normal line passing through the point on the outermost upper edge of the biological sample container is the first region, and the area outside (lower) of the normal line is the second region. Figure 25 The inner side is the lower side, and the outer side is the upper side. The second light source 6 is located within the second area. When the camera 2 is located at the bottom of the stage 3 and / or the second light source 6 is located at the bottom of the stage 3, the stage 3 is a light-transmitting stage to facilitate light transmission and shooting. Of course, when the camera 2 is located at the top inside the device body 1, the stage 3 can also be configured as a light-transmitting stage.

[0041] Figure 1-11 , Figure 17-22 The camera 2 is located inside the top of the device body 1. Figure 31 shows the camera 2 located inside the device body 1 at the bottom of the stage.

[0042] To accommodate biological sample containers 5 of different sizes, the second region is the area covered by the largest-sized biological sample container.

[0043] In this embodiment, see Figure 1-225. The second light source 6 and the camera 2 are positioned on the same side of the stage 3, and between the camera 2 and the stage. Figure 1-2 The imaging camera 2 is positioned above the stage 3, and the second light source 6 is positioned between the imaging camera 2 and the stage, in the second region below the imaging camera 2. The emitting surface of the second light source 6 is tilted towards the lid of the biological sample container on the stage 3. Figure 25 In this structure, the camera 2 is positioned below the stage 3, and the second light source 6 is positioned between the camera 2 and the stage, located in the second region above the camera 2. In this structure, the second light sources 6, illuminating from multiple specific directions at close range, can form strongly directional dark-field illumination on the surface of the petri dish when operating independently. However, due to the compactness of the light sources, each individual image exhibits a significant illuminance gradient. Simultaneously, through precise timing control by the controller, the camera captures a sequence of images with staggered "bright" and "dark" areas. This provides crucial raw data for subsequent algorithms to perform complementary synthesis using areas with moderate illuminance in each image, fundamentally changing the approach to solving the problem of uneven illuminance, shifting from pursuing absolute uniformity in a single physical illumination to utilizing the complementary information from multiple non-uniform illuminations. Figure 24-31 The camera 2 is positioned below the stage 3, and the second light source 6 is positioned between the camera 2 and the stage, in the second area above the camera 2. Figure 24-31 In this process, the second light source 6 can be positioned on the same side or opposite side of the stage 3 as the camera 2, as needed.

[0044] In some embodiments, such as Figure 7-8 As shown in Figure 27, a backlight 7 is also included, the area of ​​which can cover the entire field of view of the camera, for transmitting light from the back of the petri dish to obtain bright-field images. The backlight 7 may be equipped with a backlight diffuser 71. In this structure, the backlight 7 provides a completely different imaging modality (bright field of view), which complements the dark field of view image provided by the second light source 6; at the same time, the controller incorporates them into a unified shooting sequence, enabling a single system to simultaneously acquire two types of images, serving contour counting and color texture analysis respectively, greatly enhancing the functionality and data richness of the device.

[0045] like Figure 3-11 In another embodiment shown, the second light source 6 and the camera 2 are positioned on opposite sides of the stage 3. The camera 2 is positioned on the upper side of the stage 3, and the second light source 6 is positioned on the lower side of the stage 3, with the emitting surface of the second light source 6 tilted towards the lid of the biological sample container mounted on the stage 3. The stage 3 must be made of a light-transmitting material (such as glass or transparent acrylic). In this structure, light penetrates from below the culture medium and is blocked and scattered by the opaque colonies, highlighting the boundary between the colonies and the transparent agar, as well as the morphology of the colony bottom; simultaneously, through... Figure 1 The combination of the above illumination methods shown (which can be achieved in a system with multiple second light sources 6) can obtain the scattered light information of the colonies from the upper and lower hemispheres, resulting in a more three-dimensional visual effect and a more comprehensive ability to capture details in the final fused image, which is impossible to achieve with single-direction illumination.

[0046] Figure 24 , 26 -28, the second light source 6 and the camera 2 are located on opposite sides of the stage 3. The camera 2 is located on the underside of the stage 3.

[0047] Of course, if necessary, the second light source 6 can be placed both above and below the stage 3.

[0048] like Figure 5-8 , Figure 27-28 As shown, to further improve the uniformity of illumination coverage, at least two second light sources 6 are provided and arranged in a ring array around the stage 3 (whether on the same side or opposite side). In this structure, the multiple second light sources 6 arranged in a ring can provide illumination to the culture dish from a nearly 360-degree circumference, ensuring that effective dark-field illumination signals can be obtained from multiple directions regardless of whether the colony is located in the center or at the edge of the culture dish. At the same time, through this nearly symmetrical layout, each region in the obtained image sequence has the opportunity to be at the "ideal" illumination distance (i.e., not overexposed) in at least one image, which greatly improves the quality of the original data and lays a solid foundation for synthesizing highly uniform images. Preferably, four second light sources 6 can be provided and arranged in a ring around the stage 3.

[0049] like Figure 9-11 As shown in Figure 26, in applications requiring a reduced device height, a first reflecting mirror 4 is also included. The camera 2 is positioned horizontally towards the first reflecting mirror 4, and the first reflecting mirror 4 reflects the image of the stage 3 area into the lens of the camera 2. In this structure, the first reflecting mirror 4 changes the optical path, allowing the camera, which originally needed to be vertically positioned, to be placed horizontally, thereby significantly compressing the vertical space of the imaging module. At the same time, through the combination of reflecting mirror imaging and multi-directional second light source 6 illumination, the core function of multi-directional image acquisition is still fully retained while achieving a flattened structure, resolving the contradiction between the limited internal space of compact devices and the integrity of imaging functions.

[0050] The second light source 6 can take the form of a surface light source (such as an LED array panel), a line light source (such as an LED strip), or a point light source (such as a high-power LED). The stage 3 is made of a light-transmitting material. In this structure, the surface light source provides soft and uniform illumination, which is beneficial for obtaining high-quality raw scattered images; the line or point light source is low-cost and highly directional, and can also achieve good results through reasonable layout and algorithm compensation; the light-transmitting stage 3 is for realizing... Figure 2 The illustrated unbalanced illumination and other complex optical path designs provide the necessary physical conditions. These interchangeable component options allow for flexible configuration of the device to meet different performance requirements and cost budgets, enhancing the product's market adaptability.

[0051] like Figure 17-22 As shown in Figures 29-31, a ring-shaped second reflector 8 is also included for convenient sample management. The second reflector 8 is arranged around the corresponding position on the stage 3, located on the outer periphery of the biological sample container 5. The ring-shaped second reflector 8 has an inner mirror surface 81, which is used to reflect the image of the barcode or label affixed to the side of the biological sample container 5 into the field of view of the camera 2. In this structure, the inner mirror surface 81 of the second reflector 8 bends the horizontal label information at an appropriate angle and reflects it into the vertical field of view of the camera, so that the camera can simultaneously capture the colony image on the side of the petri dish facing the camera and the label image on the side in a single exposure; at the same time, by seamlessly integrating the label imaging function with the core dark-field imaging system, the additional ID shooting station or the action of robotic arm grasping and flipping is avoided. Without increasing the imaging time, the automatic and reliable association of sample information and image data is realized, which greatly improves the efficiency of the entire process automation.

[0052] in Figure 17-18 In the structure 30, the second reflector 8 is located below the stage. In this structure, the second reflector 8 is located below the stage 3 to avoid direct contact with the biological sample container 5, thus preventing contamination and damage. At the same time, the optical path angle is optimized through the lower reflection path, so that the reflected image of the side mark is more in line with the viewing angle of the camera 2, reducing image distortion and ensuring the accuracy of the collected data. It is suitable for various container types and experimental environments. The inner mirror surface 81 of the second reflector 8 located below is set to have a certain angle with the axis of the stage.

[0053] in Figures 19-20 In section 31, the second reflector 8 is positioned above the stage. By adopting the above technical solution, the second reflector 8 positioned above can cover a wider reflection angle, adapt to biological sample containers 5 of different heights, and match the viewing angle of the camera 2, further improving the flexibility and adaptability of identification and collection.

[0054] in Figure 21-22In section 29, the second reflector 8 is disposed on the upper surface of the stage 3 and surrounds the biological sample container 5. In this structure, the second reflector 8 directly surrounds the biological sample container 5, and the second reflector 8 is set at the same or approximately the same height as the biological sample container 8, maximizing the use of space and ensuring that the side markings are reflected to the imaging camera 2 from all directions; at the same time, by being designed to be disposed on the outside of the container 3, the authenticity and stability of the reflected image can be maintained, and it is fixedly matched with the stage 3, enhancing the overall structural compactness and durability. It also facilitates the centered placement of the biological sample container 5 inside the ring-shaped second reflector 8, further benefiting the scanning of the outer wall of the biological sample container 5.

[0055] Referring to the accompanying drawings, the working principle of this invention is as follows: The controller sequentially illuminates multiple second light sources 6 illuminating from different directions, and triggers the camera 3 to capture images each time it is illuminated, thereby obtaining a set of original images. In this set of images, for any point on the petri dish, when it is close to a lit light source, it will be overexposed in the corresponding image due to excessive illumination (becoming invalid information); however, when other light sources are illuminated, the point is farther away from the light source, the illumination is moderate, and the image quality is good. The core idea of ​​the image fusion algorithm is to "discard" overexposed pixels in each original image and "adopt" the pixel values ​​of that point that have good quality in other images. Through functions f such as "minimum value" and "weighted average", the algorithm automatically completes this selection and fusion, ultimately generating a dark-field composite image with a uniform overall background, clear colony details, and high contrast. Therefore, this invention uses a technical approach of "multi-directional compact illumination + sequential image acquisition + intelligent pixel fusion" to compensate for the uneven physical illumination caused by the size and distance limitations of the light source in the hardware with software algorithms, thereby achieving a uniform dark-field imaging effect comparable to a large ring light source in a miniaturized, low-power device.

[0056] Figure 12-15 Four RGB color images were captured under different lighting conditions when the four secondary light sources 6 were lit simultaneously using the method described above. The minimum value of each channel at the corresponding pixel level was directly taken from each of the four images to obtain a clear image of the entire culture dish without reflections, highlights, or overexposure. Figure 16 As shown, the overall background is uniform, the colonies are clearly visible, and the contrast with the background is high, which can be used for subsequent analysis by automatic colony counting software.

[0057] In the description of this invention, it should be understood that the directions or positional relationships indicated by up, down, left, right, top, bottom, same side, opposite side, etc., are based on the orientation or positional relationships shown in the accompanying drawings, and are only for the purpose of more clearly describing the technical solutions of this invention, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and should not be construed as a limitation of this invention.

[0058] Although specific embodiments of the invention have been described in detail herein, they are given for illustrative purposes only and should not be construed as limiting the scope of the invention. Various substitutions, alterations, and modifications can be conceived without departing from the spirit and scope of the invention.

Claims

1. A multi-source image fusion dark-field imaging device for biological sample containers, comprising a device body, a stage within the device body, and a camera disposed at the top of the device body or at the bottom of the stage within the device body for capturing images of the stage area, characterized in that: The device body is equipped with at least two second light sources, each of which illuminates the lid of a biological sample container placed on the stage area at a different angle. It also includes a controller that controls the second light sources to illuminate in turn according to a preset sequence and time interval, and controls the camera to perform exposure and capture when the corresponding second light source is illuminated. When the camera is positioned at the bottom of the stage and / or the second light source is positioned at the bottom of the stage, the stage is a light-transmitting stage. The second light sources and the camera are positioned on the same side and / or opposite sides of the stage. The camera captures images along the outermost edge of the biological sample container. The area inside the normal passing through the outermost edge of the biological sample container is a first region, and the area outside the normal is a second region. The second light source is positioned within the second region.

2. The multi-source image fusion dark-field imaging device for biological sample containers as described in claim 1, characterized in that: It also includes at least one backlight that can cover the entire field of view for illuminating the back of the petri dish to obtain a bright-field image.

3. The multi-source image fusion dark-field imaging device for biological sample containers as described in claim 1, characterized in that: The second light source and the camera are located on the same side of the stage, between the camera and the stage, and the light-emitting surface of the second light source is tilted towards the lid of the biological sample container on the stage.

4. The multi-source image fusion dark-field imaging device for biological sample containers as described in claim 1, characterized in that: The second light source and the camera are located on opposite sides of the stage. The camera is located on the upper side of the stage, and the second light source is located on the lower side of the stage. The light-emitting surface of the second light source is tilted towards the lid of the biological sample container on the stage.

5. The multi-source image fusion dark-field imaging device for biological sample containers as described in claim 4, characterized in that: The second light source is provided in at least three, and the at least three second light sources are arranged in a ring.

6. The multi-source image fusion dark-field imaging device for biological sample containers as described in claim 1, characterized in that: It also includes a first reflector that reflects the stage area to the camera, the camera being positioned toward the first reflector.

7. The multi-source image fusion dark-field imaging device for biological sample containers as described in claim 1, characterized in that: The second light source is a surface light source, a line light source, and / or a point light source.

8. The multi-source image fusion dark-field imaging device for biological sample containers as described in claim 1, characterized in that: It also includes a ring-shaped second reflector, which is arranged around the biological sample container at a corresponding position on the stage. The ring-shaped second reflector is equipped with a mirror surface for reflecting the side markings of the biological sample container on the stage to the inner mirror surface of the camera, so that the camera can simultaneously capture the sample image of the side of the biological sample container facing the camera and the image of the markings on the side of the biological sample container.

9. The multi-source image fusion dark-field imaging device for biological sample containers as described in claim 1, characterized in that: The second region is the area covered by the largest-sized biological sample container.

Images