Analytical support, immunochromatographic test strip and method for manufacturing analytical support

Abstract

An analytical carrier (1) includes a porous body (10) and a support body (20) that supports the porous body (10) on one side surface, wherein the porous body (10) includes a skeleton (11) formed by a plurality of hollow particles (13) and a plurality of voids (12) surrounded by the skeleton (11), the hollow particles (13) have a shell (14) including an anodized film containing alumina and a cavity (15) surrounded by the shell (14), and an L value in the Lab color system when a detector is used to measure a surface of the porous body (10) in the analytical carrier (1) placed on a white reflection standard corrected for the detector is 80 or more after the porous body (10) is water absorbed.

Description

Technical Field

[0001] This disclosure relates to analytical carriers, immunochromatographic test strips, and methods for manufacturing analytical carriers. Background Technology

[0002] Immunochromatography (immunochromatographic assay) is a known analytical method for analyzing analytes mounted on an analytical carrier. Lateral flow assay kits utilizing immunochromatography are also known. These kits include test strips containing labeled antibodies that react with the analyte and are labeled with particles such as gold colloidal particles and latex particles, as well as capture antibodies that react with the analyte and are immobilized at a predetermined site on the analytical carrier.

[0003] In this test kit, for example, when a sample containing an antigen, the analyte, is collected from a living organism, and a solution containing the sample is added to a designated position on the test kit, the antigen reacts with a labeled antibody to form a complex. The solution containing the complex then flows through an analytical carrier via capillary action. Furthermore, as the solution containing the complex unfolds on the analytical carrier, the antigen contained in the complex is captured by a capture antibody on the analytical carrier, causing the complex to aggregate at the capture site, thus producing color development. The degree of color development at the capture site, where the complex is supported on the analytical carrier, is visually confirmed to determine whether the sample contains the antigen.

[0004] Patent Document 1 discloses an aluminum component having a porous body comprising a framework formed by aggregates of multiple aluminum particles and multiple voids surrounded by the framework. The framework contains a shell containing alumina, and the surface of the framework is formed by the shell. The aluminum component of Patent Document 1 has an aluminum substrate supporting the porous body, with the porous body disposed on one or both sides of the substrate. Existing technical documents Patent documents

[0005] Patent Document 1: International Publication No. 2021 / 079813 Summary of the Invention

[0006] The aluminum component in Patent Document 1 is suitable for use as a test strip for immunochromatography due to its high whiteness and water absorption capacity. However, in the aluminum component of Patent Document 1, the porous body becomes transparent when it absorbs a solution containing the sample, and the gray color of the aluminum substrate supporting the porous body is visible through the porous body, causing the aluminum component to sometimes appear gray. Therefore, it is sometimes difficult to visually identify the signal (line) on the aluminum component. Furthermore, even when the signal is read using a measuring device, the signal-to-noise ratio (SN ratio) may decrease due to increased background values. That is, in the aluminum component of Patent Document 1, the discriminative power of the signal on the porous body may decrease after the porous body absorbs water.

[0007] This disclosure was made based on the technical problems existing in the prior art. Furthermore, the object of this disclosure is to provide an analytical carrier, an immunochromatographic test strip, and a method for manufacturing the analytical carrier, which can improve the discriminative power during water absorption.

[0008] The analytical carrier of the first embodiment disclosed herein comprises a porous body and a support body supporting the porous body on one side surface. The porous body comprises a framework formed by a plurality of hollow particles and a plurality of pores surrounded by the framework. Each hollow particle has a shell containing an anodized film comprising alumina and pores surrounded by the shell. The framework is formed continuously by the shells of the plurality of hollow particles. The porosity of the porous body is 50% by volume or more and less than 100% by volume. The average pore size of the porous body is 0.1 μm or more and less than 20 μm. When the analytical carrier, after absorbing water from the porous body, is placed on a white reflectance standard calibrated by the measuring instrument, the L value of the surface of the porous body in the analytical carrier is measured using the measuring instrument. a b L in the color system The value is above 80.

[0009] The second type of immunochromatographic test strip disclosed herein includes an analytical carrier.

[0010] The third aspect of the analytical carrier disclosed herein is a method for manufacturing an analytical carrier comprising a porous body and a support body supporting the porous body on one side surface. The porous body comprises a framework formed by a plurality of hollow particles and a plurality of voids surrounded by the framework. Each hollow particle has a shell containing an anodized film comprising aluminum oxide and voids surrounded by the shell. The framework is formed continuously by the shells of the plurality of hollow particles. The manufacturing method includes a sintering step, wherein a plurality of aluminum metal particles are sintered on an aluminum substrate to obtain a sintered material comprising an aluminum substrate and a sintered body formed by stacking aluminum metal particles on the aluminum substrate. The manufacturing method includes an anodizing step, wherein the sintered material is anodized to form a shell containing an anodized film on the surface of the aluminum metal particles. The manufacturing method includes a dissolution step, wherein the aluminum metal particles surrounded by the shell are dissolved. In the above manufacturing method, by repeatedly performing the anodizing and dissolution steps, a metal component with a porous body stacked on an aluminum substrate is formed. The above manufacturing method includes a lamination process, wherein the lamination process involves bonding a porous metal component to a support to form a laminate consisting of an aluminum substrate, a porous material, and a support, which are sequentially stacked. The above manufacturing method includes a peeling process, wherein the peeling process involves dissolving the aluminum substrate and peeling it off from the laminate. The aluminum metal particles comprise at least one type of aluminum selected from high-purity aluminum, pure aluminum, and aluminum alloys. The aluminum substrate comprises at least one type of aluminum selected from high-purity aluminum, pure aluminum, and aluminum alloys. The average particle size of the plurality of aluminum metal particles is 0.1 μm or more and 20 μm or less. The filling rate of the sintered body is 10% by volume or more and 60% by volume or less. The L value is measured using a measuring instrument on the surface of the support for bonding the porous material, with the support placed on a white reflectance standard calibrated before bonding the porous material. a b L in the color system The value is above 80.

[0011] According to this disclosure, an analytical carrier, an immunochromatographic test strip, and a method for manufacturing the analytical carrier can be provided, which can improve the discriminative power of water absorption. Attached Figure Description

[0012] Figure 1 This is a schematic cross-sectional view illustrating an example of an analytical carrier for one embodiment. Figure 2 This is a perspective view illustrating an example of a test kit according to one embodiment. Figure 3 This is a cross-sectional view showing an example of a sintered material obtained by sintering multiple aluminum metal particles on an aluminum substrate. Figure 4This is a cross-sectional view illustrating an example of a state in which anodizing of sintered materials results in the formation of an outer shell containing an anodized film on the surface of aluminum metal particles. Figure 5 This is a cross-sectional view illustrating an example of the state in which aluminum metal particles surrounded by a shell dissolve. Figure 6 This is a cross-sectional view illustrating an example of a laminated structure formed by bonding a porous metal component to a support. Figure 7 This is a schematic diagram illustrating an example of the first stacking method. Figure 8 This is a schematic diagram illustrating an example of the second stacking method. Figure 9 This is a schematic diagram illustrating an example of the third stacking method. Figure 10 This is a schematic diagram illustrating an example of the fourth stacking method. Figure 11 This is a cross-sectional view illustrating an example of the state in which the aluminum substrate is dissolved and peeled off from the laminate. Figure 12 This is a SEM (scanning electron microscope) image of the cross-section of the porous carrier in Example 5. Figure 13 This is an SEM image of the surface of the porous carrier in Example 5. Figure 14 This is a graph showing the relationship between the pore diameter and the differential pore surface area for Example 5 and the Reference Example. Figure 15 This is a graph showing the relationship between the pore diameter and the cumulative pore surface area for Example 5 and the Reference Example. Figure 16 It is a top view schematically showing the state before the bending test is carried out. Figure 17 It is a side view schematically showing the state of the analytical carrier being bent during a bending test. Figure 18 This is a top view schematically showing the state of the test strips used in the embodiment. Figure 19 These are photographs showing the results of gold colloid tests for Example 5 and the Reference Example. Detailed Implementation

[0013] The analytical carrier, immunochromatographic test strip, and method for manufacturing the analytical carrier according to this embodiment will be described in detail below with the help of the accompanying drawings. This disclosure is not limited to the following embodiments. Furthermore, some or all of the constituent elements in the embodiments can be suitably combined. Additionally, the dimensions in the drawings are exaggerated for ease of explanation and may sometimes differ from the actual dimensions.

[0014] [1. Analytical medium] First, use Figure 1 The analysis of this embodiment will be explained using carrier 1. Figure 1 This is a schematic cross-sectional view showing an example of the analytical carrier 1. For example... Figure 1 As shown, the analytical carrier 1 includes a porous material 10 and a support 20 supporting the porous material 10 on one side surface. Normally, in the dry state of the porous material 10, the refractive index of the alumina constituting the porous material 10 differs greatly from that of air. Therefore, light incident on the porous material 10 travels through the air layer while being repeatedly refracted, resulting in a higher proportion of light reflected from the porous material 10 and less light reaching the support 20, thus making the porous material 10 appear white. On the other hand, when the porous material 10 absorbs water, the refractive index of water is closer to that of alumina than that of air. Therefore, the refraction of light between the porous material 10 and the water layer decreases, and more light reaches the support 20. Thus, when the support 20 is an aluminum substrate, if the porous material 10 absorbs water, the analytical carrier 1 appears gray due to the gray color of the support 20. Therefore, it is understood that the analytical carrier 1 of this embodiment, by using the support 20 to support the porous body 10 and in a state where the porous body 10 has absorbed water, allows the L of the analytical carrier 1 to be placed in the water. Setting the value to 80 or higher can improve the discriminative power of water absorption. The analytical carrier 1 of this embodiment will now be described in detail.

[0015] <Porous body> The porous material 10 includes a framework 11 and a plurality of voids 12 surrounded by the framework 11. The framework 11 is formed by the aggregation of a plurality of hollow particles 13. The voids 12 are formed by the internal space of the porous material 10 surrounded by the framework 11 or the plurality of hollow particles 13. The hollow particles 13 have shells 14 and cavities 15 surrounded by the shells 14. The framework 11 includes shells 14, and the surface of the framework 11 is formed by the shells 14. The framework 11 is formed by the continuous shells 14 of the plurality of hollow particles 13. The hollow particles 13 are connected through the shells 14, thereby forming a three-dimensional mesh structure framework 11. The shells 14 are disposed on the outer surface side of the porous material 10.

[0016] The outer shell 14 of the hollow particle 13 may have a through hole 16 extending through the outer shell 14 in the thickness direction. The through hole 16 can connect the cavities 15 on the inner side of the hollow particle 13 separated by the outer shell 14 with the gaps 12 on the outer side of the hollow particle 13. In addition, the through hole 16 can also connect the cavities 15 of adjacent and connected hollow particles 13 to each other. The cavities 15 can be connected to the outside of the hollow particle 13 or the framework 11 through the through hole 16. When the porous body 10 is immersed in a liquid containing a sample or the like, the liquid can flow into or out of the cavities 15 through the through hole 16 of the outer shell 14. In addition, the cavities 15 contained in adjacent hollow particles 13 are connected. Therefore, when the porous body 10 is immersed in a liquid, the liquid passes through the through hole 16 and through the cavities 15, thereby flowing inside the framework 11.

[0017] Here, in immunochromatography, as described later, labels such as colored particles, gold colloidal particles, or fluorescent beads are typically used. When the analytical carrier 1 is used, for example, as an immunochromatographic test strip, the porous body 10 can draw up the dispersed labeled solution via capillary action. The mechanism is not clear at this point, but it is presumed that the dispersed labeled solution permeates into the pores 12 and voids 15 present inside the porous body 10 and is partially transferred to the surface of the porous body 10. Furthermore, although drawup is mainly achieved by the flow of the solution in the pores 12, it is presumed that the flow of the solution in the voids 15 and through-holes 16 also facilitates drawup.

[0018] Thus, the porous body 10 becomes a porous body with pores 12 communicating with the outside. At this time, the pores 12 are surrounded by a shell 14. That is, the pores 12 are formed by the framework 11 inside the porous body 10 or by the shell 14 formed on its surface. The pores 12 forming a single-cell structure surrounded by the shell 14 can communicate with pores 12 forming other single-cell structures. Specifically, the porous body 10 can be an open-cell structure. Furthermore, a single pore 12 or multiple pores 12 can extend from one side of the porous body 10 to the other, or they can not.

[0019] The average particle size of the multiple hollow particles 13 is 0.1 μm to 20 μm. By setting the average particle size to 0.1 μm or more, the strength of the porous material 10 is improved, and breakage during transport can be suppressed. By setting the average particle size to 20 μm or less, the flow rate of the analytical carrier 1 can be increased. The average particle size can be 1 μm or more, 3 μm or more, or 5 μm or more. The average particle size can be 18 μm or less, 15 μm or less, or 10 μm or less.

[0020] The average particle size of the multiple hollow particles 13 can be determined by observing the cross-section of the multiple porous bodies 10 using a scanning electron microscope. For example, after sintering, some aluminum metal particles melt and become connected, but the approximately circular portions can be approximated as circular. Therefore, in the above cross-sectional observation, the maximum diameter (major diameter) of each approximately circular aluminum metal particle is taken as the particle size, and the particle size of any 50 aluminum metal particles is measured. Their arithmetic mean is taken as the average particle size of the sintered aluminum metal particles. The average particle size of the multiple aluminum metal particles before sintering is the D50 value obtained by measuring the particle size distribution on a volume basis using laser diffraction. In addition, secondary particles of aluminum metal particles are hardly formed in the range of 0.1 μm to 20 μm. Therefore, the average particle size of the sintered hollow particles 13 observed using a scanning electron microscope is almost the same as the average particle size of the aluminum metal particles before sintering determined by laser diffraction.

[0021] The shape of each hollow particle 13 is not particularly limited and can be spherical, polygonal, irregular, scaly, or fibrous, etc. Among these, the shape of the hollow particle 13 is preferably spherical. If the hollow particle 13 is spherical, the size of the multiple pores 12 becomes equal. For example, in immunochromatography, as described later, stained particles, gold colloidal particles, or fluorescent beads are used for labeling. Moreover, if the pores 12 are of equal size, such labels will not be trapped within the pores 12 and can flow smoothly within the porous body 10. Furthermore, the spherical shape mentioned here is not limited to round spheres but can also be a sphere with slightly uneven surfaces. Additionally, the spherical shape described here can include not only spheres with an aspect ratio of 1 but also hollow particles 13 with an aspect ratio of 1 or greater. The aspect ratio of the hollow particle 13 can be 5 or less, 3 or less, or 2 or less. Furthermore, the aspect ratio is the ratio of the major axis to the minor axis of the hollow particle 13 and is the average value of the multiple hollow particles 13.

[0022] The housing 14 includes an anodic oxide film comprising aluminum oxide. The anodic oxide film included in the housing 14 can be a barrier-type anodic oxide film or a porous anodic oxide film. The housing 14 can be formed solely of a barrier-type anodic oxide film, or it can be formed of a double-layered anodic oxide film with a porous anodic oxide film formed further outer of the barrier-type anodic oxide film. The housing 14 can have a hydrated film comprising aluminum hydroxide. The hydrated film can be formed by a hydration treatment process described later.

[0023] The ratio of metallic aluminum contained in the porous body 10 to the aluminum-containing components constituting the porous body 10, by mass ratio, can be 0 or more and 0.1 or less. In other words, the ratio of the mass of metallic aluminum contained in the porous body 10 to the mass of the aluminum-containing components constituting the porous body 10, by mass ratio, can be 0 or more and 0.1 or less. The mass of the aluminum-containing components constituting the porous body 10 refers to the total mass of alumina, aluminum hydroxide, electrolyte anions, resin residue (coke residue), and metallic aluminum contained in the porous body 10. By setting the ratio of metallic aluminum to 0.1 or less, even if the porous body 10 absorbs water, the light transmittance of the porous body 10 increases, thereby reducing the graying caused by aluminum metal. This improves the discernibility when water is absorbed. The ratio of metallic aluminum is preferably 0.05 or less, more preferably 0.01 or less, and even more preferably 0.001 or less. The ratio of metallic aluminum contained in the porous body 10 to the aluminum-containing components constituting the porous body 10 can be calculated based on JIS G2404:2022. Furthermore, the outer shell 14 of the hollow particles 13 may also be substantially composed solely of an anodized film containing aluminum oxide.

[0024] The thickness of the outer shell 14 is preferably between 40 nm and 1000 nm. By setting the thickness of the outer shell 14 to 40 nm or more, damage to the porous material 10 can be suppressed even when the analytical carrier 1 is bent. This easily prevents the outer shell 14 from being damaged and leaving wrinkles when the analytical carrier 1 is bent or deformed. Furthermore, by setting the thickness of the outer shell 14 within this range, an analytical carrier 1 with sufficiently high corrosion resistance can be provided. The thickness of the outer shell 14 can be 100 nm or more, 200 nm or more, or 400 nm or more. Furthermore, the thickness of the outer shell 14 can be 900 nm or less, 700 nm or less, or 500 nm or less. By keeping the thickness of the outer shell 14 below the above-mentioned upper limit, when the outer shell is formed by alternately repeating the anodizing and dissolution processes, the anodized film will not become too thick, allowing the aluminum portion of the metal to dissolve and form an outer shell of the desired thickness, thus easily improving production efficiency. The thickness of the outer shell 14 can be measured, for example, by observing the cross-section of the outer shell 14 using a scanning electron microscope or the like.

[0025] The porous body 10 has a cumulative pore surface area of ​​pores with a diameter of 0.1 μm or more and 100 μm or less, which can be 0.1 m². 2 / cm 3 Above and 20m 2 / cm 3 The cumulative pore surface area of ​​porous material 10 is 0.1 m². 2 / cm 3Under the above conditions, the amount of antibody adsorbed on the porous plast 10 increases. This improves the absorbance of the analytical carrier 1. The total pore surface area of ​​the porous plast 10 is 20 m². 2 / cm 3 The water absorption capacity of porous material 10 can be improved under the following conditions: The cumulative pore surface area can reach 0.5 m². 2 / cm 3 The above can also be found in 1m 2 / cm 3 That's all. Furthermore, the cumulative pore surface area can reach 10m². 2 / cm 3 The following can also be done at 6m 2 / cm 3 The cumulative pore surface area of ​​the porous body 10 can be obtained by converting the pore volume of pores with a pore diameter of 0.1 μm or more and 100 μm or less, as determined by mercury infiltration method, into surface area for each pore diameter and then accumulating the total pore volume.

[0026] The porosity of the porous body 10 is 50% by volume or more and less than 100% by volume. By setting the porosity of the porous body 10 to 50% by volume or more, the water absorption performance can be improved. By setting the porosity to less than 100% by volume, the peeling of hollow particles 13 from the porous body 10 can be suppressed, thereby improving the strength of the porous body 10. The porosity can be 60% by volume or more, 70% by volume or more, 80% by volume or more, 90% by volume or more, or 95% by volume or more. In addition, the porosity can be less than 95% by volume, less than 90% by volume, or less than 80% by volume. The porosity of the porous body 10 can be obtained by dividing the cumulative pore volume of the porous body 10 by the volume of the porous body 10. The cumulative pore volume of the porous body 10 can be obtained by accumulating the pore volume of pores with a pore diameter of 0.1 μm or more and less than 100 μm as determined by mercury infiltration method.

[0027] The average pore size of the porous body 10 is 0.1 μm or more and 20 μm or less. By setting the average pore size to 0.1 μm or more, the water absorption performance of the analytical carrier 1 can be improved. Here, the particle size of the labels such as commonly used colored particles, gold colloidal particles, or fluorescent beads used in immunochromatography is about 10 nm to 50 nm, and there are also labels with larger particle sizes. When the analytical carrier 1 is used, for example, in an immunochromatographic test strip, by setting the average pore size to 0.1 μm or more, the trapping of such labels in the pores 12 can be suppressed, and the labels can flow smoothly within the porous body 10. Furthermore, by setting the average pore size to 20 μm or less, the water absorption performance of the analytical carrier 1 can be improved. The average pore size can be 0.5 μm or more, 1 μm or more, 3 μm or more, or 5 μm or more. In addition, the average pore size can be 15 μm or less, 12 μm or less, 10 μm or less, or 9 μm or less. The average pore size of the porous material 10 can be calculated using the following mathematical formula. The average pore diameter of porous material 10 = 4 × (cumulative pore volume of porous material 10) / (cumulative pore surface area of ​​porous material 10) The cumulative pore volume and cumulative pore surface area of ​​the porous body 10 can be obtained as described above.

[0028] The ratio of the cumulative pore surface area of ​​pores with a pore size of 0.1 μm or more and less than 1 μm to the cumulative pore surface area of ​​pores with a pore size of 1 μm or more and less than 10 μm can be 0.1 or more and 10 or less. The ratio of the cumulative pore surface areas can be 0.2 or more, 0.5 or more, or 1 or more. The ratio of the cumulative pore surface areas can be 8 or less, 6 or less, or 5 or less. The cumulative pore surface area of ​​pores with a pore size of 1 μm or more and less than 10 μm can be obtained by accumulating the pore surface areas of pores with a pore size of 1 μm or more and less than 10 μm. Similarly, the cumulative pore surface area of ​​pores with a pore size of 0.1 μm or more and less than 1 μm can be obtained by accumulating the pore surface areas of pores with a pore size of 0.1 μm or more and less than 1 μm. The cumulative pore surface area of ​​the porous body 10 can be determined by mercury infiltration as described above. The absorbance and other signals from the analytical carrier 1 tend to be proportional to the flow rate. Furthermore, to reduce the flow rate, or in other words, to accelerate water absorption, it is necessary to increase the porosity. That is, in the analytical carrier 1, where high porosity and rapid water absorption occur, a trade-off occurs where the signal weakens. By setting the ratio of the cumulative pore surface area above the aforementioned lower limit, the surface area of ​​the porous body 10 increases, the amount of antibody bound to the porous body 10 increases, and the signal from the analytical carrier 1 becomes stronger. Additionally, even if the flow rate of the analytical carrier 1 is the same as that of the nitrocellulose membrane, a higher absorbance than that of the nitrocellulose membrane can be obtained by setting the ratio of the cumulative pore surface area above the aforementioned lower limit. By setting the ratio of the cumulative pore surface area below the aforementioned upper limit, clogging of the labeled particles can be prevented.

[0029] The thickness of the porous substrate 10 is preferably 20 μm or more and 1 mm or less. By setting the thickness of the porous substrate 10 to 20 μm or more, it is easy to ensure sufficient thickness for water absorption by capillary action. By setting the thickness of the porous substrate 10 to 1 mm or less, the analytical support 1 can be prevented from appearing gray when the porous substrate 10 absorbs water. The thickness of the porous substrate 10 can be 190 μm or less. By setting the thickness of the porous substrate 10 to 190 μm or less, it is possible to prevent the porous substrate 10 from cracking and leaving wrinkles when the analytical support 1 is bent. The thickness of the porous substrate 10 can be 30 μm or more, 40 μm or more, or 50 μm or more. The thickness of the porous substrate 10 can be 150 μm or less, or 100 μm or less.

[0030] The porous body 10 may be composed of a single porous layer, or it may contain multiple porous layers. For example, the porous body 10 may have a first porous layer and a second porous layer disposed on the surface of the first porous layer. In the first and second porous layers, any of the following may differ: the average particle size, aspect ratio, or compositional material, porosity of the porous body 10, or average pore size. For example, the average particle size of the hollow particles 13 contained in the first porous layer may be larger than the average particle size of the hollow particles 13 contained in the second porous layer. Furthermore, the thicknesses of the first and second porous layers may be the same or different.

[0031] The porous body 10 may include a coating layer 17 disposed on the surface opposite to the support 20, separating the inner side of the porous body 10 containing the framework 11 and voids 12 from the outer side of the porous body 10, and containing an anodized film comprising alumina. The coating layer 17 may have connecting holes 18 that connect the inner side and the outer side of the porous body 10. The coating layer 17 may be continuously formed with the shell 14 of the hollow particles 13. The cavities 15 of the hollow particles 13 can communicate with the outer side of the porous body 10 through the connecting holes 18. By including the coating layer 17 in the porous body 10, the exposure of the hollow particles 13 to the outside and direct contact between the hollow particles 13 and fingers can be prevented. This can suppress the peeling of the hollow particles 13. Therefore, the operability of the analytical carrier 1 can be improved.

[0032] The surface roughness Sa of the porous material 10 can be 0.01 μm or more and less than 1.8 μm. When the surface roughness Sa of the porous material 10 is less than 1.8 μm, there is a tendency that hollow particles 13 will not be exposed on the surface of the porous material 10, and the surface of the porous material 10 is covered by the coating layer 17. Therefore, the hollow particles 13 of the porous material 10 are not easily peeled off from the porous material 10, the support 20, or the analytical carrier 1. The surface roughness Sa of the porous material 10 can be 0.1 μm or more, 0.2 μm or more, 0.4 μm or more, 0.6 μm or more, or 0.8 μm or more. Furthermore, the surface roughness Sa of the porous material 10 can be less than 1.5 μm, less than 1.2 μm, or less than 1 μm. The arithmetic mean roughness Sa can be obtained by measuring the surface of the porous material 10 opposite to the support 20 according to ISO 25178.

[0033] <Support> The support 20 supports the porous body 10 on one side surface. By using the support 20 to support the porous body 10, damage to the porous body 10 due to deformation or other reasons can be prevented. The support 20 may include an adhesive layer 22 that is bonded to the porous body 10. The adhesive layer 22 may be bonded to the outer shell 14 of the porous body 10.

[0034] The support 20 may include an adhesive layer 22 bonded to the porous body 10, and a support layer 21 supporting the adhesive layer 22. In this case, the adhesive layer 22 is sandwiched between the porous body 10 and the support layer 21. By using the adhesive layer 22 to bond the porous body 10 and the support layer 21, the porous body 10 and the support layer 21 can be firmly bonded together. Alternatively, the support 20 may not include the support layer 21, and may be constituted by the adhesive layer 22.

[0035] The adhesive layer 22 has an adhesive portion 23, in which the material constituting the adhesive layer 22 enters the voids 12 and cavities 15 of the porous body 10, contacts the hollow particles 13 and the outer shell 14, and adheres to the hollow particles 13 and the outer shell 14. The adhesive portion 23 is a part formed by the material constituting the adhesive layer 22 being mixed with the hollow particles 13 and the outer shell 14 along the adhesive interface direction between the porous body 10 and the adhesive layer 22.

[0036] The adhesive layer 22 may have an adhesive portion 23 and an auxiliary portion 24. The auxiliary portion 24 is made of the same material constituting the adhesive layer 22, is continuous with the adhesive portion 23, and backs the adhesive portion 23 from the side opposite to the porous body 10, thereby assisting in adhesion. The auxiliary portion 24 is a portion formed by the presence of the material constituting the adhesive layer 22 along the bonding interface direction between the porous body 10 and the adhesive layer 22. The auxiliary portion 24 is disposed in the adhesive layer 22 on the side opposite to the adhesive portion 23 that contacts the porous body 10. The auxiliary portion 24 functions as a layer that bonds the adhesive portion 23 to the support layer 21 by being disposed between the adhesive portion 23 and the support layer 21 and bonding the auxiliary portion 24 to the support layer 21. Furthermore, the auxiliary portion 24 also functions as a layer that bonds the adhesive layer 22 to the support layer 21.

[0037] For example, when using thermoplastic resin as the adhesive layer 22 and bonding the adhesive layer 22 to the porous body 10, when the heated adhesive layer 22 comes into contact with the porous body 10, the portion of the softened thermoplastic resin in contact with the porous body 10 deforms and enters the voids 12 and cavities 15 of the porous body 10, and then solidifies as the temperature decreases. At this time, the thermoplastic resin enters the voids 12 and cavities 15 of the porous body 10, thus defining the portion of the layer in which the thermoplastic resin, hollow particles 13, and outer shell 14 are mixed and in contact as the adhesive portion 23. On the other hand, when bonding the adhesive layer 22 to the porous body 10, the portion of the thermoplastic resin that does not come into contact with the porous body 10 but maintains the thermoplastic resin, and which is continuous with the adhesive portion 23, is defined as the auxiliary portion 24.

[0038] Furthermore, for example, when using an adhesive as the bonding layer 22 and bonding the bonding layer 22 to the porous body, when the bonding layer 22 comes into contact with the porous body 10, the portion of the adhesive that comes into contact with the porous body 10 deforms and enters the voids 12 and cavities 15 of the porous body 10. At this time, the portion of the layer in which the adhesive, hollow particles 13, and shell 14 are mixed and in contact is designated as the bonding portion 23. On the other hand, when bonding the bonding layer 22 to the porous body 10, the portion of the adhesive that does not come into contact with the porous body 10 but maintains the adhesive, and is continuous with the bonding portion 23, is designated as the auxiliary portion 24.

[0039] The thickness of the adhesive layer 22 can be 1 μm or more, 5 μm or more, 10 μm or more, 20 μm or more, or 30 μm or more. Alternatively, the thickness of the adhesive layer 22 can be less than 1000 μm, 500 μm or less, 300 μm or less, 200 μm or less, 100 μm or less, 50 μm or less, or 40 μm or less. The thickness of the adhesive layer 22 is generally greater than the thickness of the adhesive portion 23. By ensuring the thickness of the adhesive layer 22 is above the aforementioned lower limit, when the adhesive layer 22 is bonded to the porous material 10 and the support 20 deforms, the adhesive layer 22 mitigates deformation and suppresses strain generated between the support 20 and the porous material 10, thereby easily maintaining the bond between the support 20 and the porous material 10. Therefore, when the support 20 deforms, it is easier to prevent the porous material 10 from peeling off from the support 20. Furthermore, when the support 20 deforms, wrinkles in the porous body 10 are easily suppressed. By keeping the thickness of the adhesive layer below the aforementioned upper limit, it bends moderately during roll-to-roll production, thus facilitating transport. The thickness of the adhesive layer 22 refers to the overall thickness of the material constituting the adhesive layer 22 in the thickness direction when viewed in cross-section in a plane orthogonal to the bonding interface direction of the porous body 10 and the adhesive layer 22.

[0040] The thickness of the adhesive portion 23 can be 1 μm or more, 2 μm or more, 3 μm or more, 4 μm or more, 5 μm or more, or 10 μm or more. Alternatively, the thickness of the adhesive portion 23 can be less than 100 μm, 50 μm or less, 40 μm or less, 30 μm or less, 20 μm or less, or 10 μm or less. By making the thickness of the adhesive portion 23 above the aforementioned lower limit, the adhesive portion 23 is connected with a thickness that is equal to or exceeds the thickness of the outer shell 14. At this time, the adhesive portion 23 penetrates deep into the outer shell 14, thereby exerting an anchoring effect, and the porous material 10 and the support 20 are easily and firmly bonded. Furthermore, the increased contact area between the outer shell 14 and the adhesive portion 23 enhances the effects of hydrogen bonds, van der Waals forces, ionic bonds, or covalent bonds provided by the adhesive portion 23, thereby easily and firmly bonding the porous material 10 and the support 20. Therefore, when the support 20 deforms, it is easy to prevent the porous material 10 from peeling off from the support 20. In addition, when the support 20 deforms, it is easy to prevent wrinkles from forming in the porous material 10. By keeping the thickness of the adhesive portion 23 below the above-mentioned upper limit, it is possible to prevent the porous material 10 from being completely filled with resin. The thickness of the adhesive portion 23 refers to the interval between the following lines when viewed in cross-section in a plane orthogonal to the bonding interface direction of the porous material 10 and the adhesive layer 22, within the area where the material constituting the adhesive layer 22 contacts the porous material 10: the line passing through the portion of the porous material 10 closest to the adhesive layer 22 (lowest part) and parallel to the bonding interface direction of the porous material 10 and the adhesive layer 22, and the line passing through the portion of the adhesive layer 22 closest to the porous material 10 (upper part) and parallel to the bonding interface direction of the porous material 10 and the adhesive layer 22.

[0041] The thickness of the support layer 21 can be thicker than the thickness of the adhesive layer 22. The thickness of the support layer 21 can be 10 μm or more, 50 μm or more, 70 μm or more, 90 μm or more, or 100 μm or more. Alternatively, the thickness of the support layer 21 can be less than 1000 μm, less than 500 μm, less than 300 μm, less than 200 μm, less than 150 μm, or less than 100 μm.

[0042] The support 20 can be flexible. As with the aluminum component described in Patent Document 1, when a porous material is disposed on the surface of an aluminum substrate, bending the analytical carrier 1 can sometimes cause plastic deformation of the aluminum substrate, preventing it from returning to its original shape. In this case, the porous material disposed on the surface of the aluminum substrate will also wrinkle along with the aluminum substrate. On the other hand, when the analytical carrier 1 is flexible, even if the analytical carrier 1 is bent and deformed, it easily returns to its original shape, thus the support 20 is less prone to wrinkling. The support 20 supports the porous material 10, and since the support 20 is less prone to wrinkling, it can suppress the formation of wrinkles in the porous material 10 of the analytical carrier 1.

[0043] The adhesive layer 22 may contain at least one selected from resin, elastomer, starch, and protein. That is, the adhesive layer 22 may be a resin adhesive layer or an elastomer adhesive layer. The resin may contain at least one selected from thermoplastic resin, thermosetting resin, and UV-curable resin. The UV-curable resin may contain at least one of free radical curing resin and cationic curing resin. The resin may contain at least one selected from polyethylene (HDPE, MDPE, LDPE), polypropylene, modified polyolefin resins (modified polypropylene, modified polyethylene, etc.), ethylene vinyl acetate copolymer, polyvinyl chloride, polyvinyl acetate, polyamide, polyester, (meth)acrylic resin, epoxy resin, polyurethane resin, fluoropolymer, styrene resin, ABS resin, silicone resin, phenolic resin, melamine resin, polyvinyl alcohol, and polycyanoacrylate. Additionally, as a resin, it may include: isocyanate-based adhesives, polyurethane resin-based adhesives, polyurethane resin solvent-based adhesives, polyurethane resin emulsion adhesives, acrylic resin-based adhesives, anaerobic acrylic resin adhesives, olefin-based adhesives, silicone-based adhesives, rubber-based adhesives, ethylene vinyl acetate resin emulsion adhesives, epoxy resin-based adhesives, epoxy resin emulsion adhesives, vinyl chloride resin solvent-based adhesives, chloroprene rubber-based adhesives, and cyanopropylene. This includes adhesives such as ester-based adhesives, water-based polymer-isocyanate-based adhesives, styrene-butadiene rubber solution-based adhesives, styrene-butadiene rubber latex adhesives, nitrile rubber-based adhesives, nitrocellulose adhesives, phenolic resin-based adhesives, modified silicone adhesives, polyimide adhesives, polystyrene resin solvent-based adhesives, polybenzimidazole adhesives, polymethyl methacrylate resin solution-based adhesives, and light-UV cured resin-based adhesives. The elastomer may include at least one selected from thermoplastic elastomers and rubbers. The adhesive layer 22 may contain an ethylene-vinyl acetate copolymer. The ethylene-vinyl acetate copolymer melts at a relatively low temperature, thus easily bonding the porous body 10 to the support layer 21. When the adhesive layer 22 contains a thermoplastic resin, the melting point of the adhesive layer 22 is preferably lower than the melting point of the support 20. From the viewpoint of suppressing the deformation of the support 20 when the carrier 1 undergoes bending deformation, the adhesive layer 22 preferably contains a flexible material.

[0044] The support layer 21 may comprise at least one of resin and glass. These materials effectively support the porous body 10. The L measured was obtained by placing the analytical carrier 1 on a white reflectance standard after the porous body 10 had absorbed water. From a value perspective, the support layer 21 preferably comprises a material with high light transmittance or high whiteness. Furthermore, from the viewpoint of suppressing deformation of the support 20 when the analytical carrier 1 undergoes bending deformation, the support layer 21 preferably comprises a material that does not easily undergo plastic deformation when a certain strain is applied. The resin may also comprise at least one selected from thermoplastic resins, thermosetting resins, and UV-curable resins. The resin may also comprise at least one selected from polyester, acrylic resin, polyurethane resin, silicone resin, epoxy resin, polyolefin, cellulose resin, styrene resin, polyimide, and polycarbonate.

[0045] The support layer 21 can be a single layer or multiple layers. When the support layer 21 is multiple layers, the materials of each layer can be the same or different. When the support layer 21 is multiple layers, the melting point of the material disposed in the support layer 21 closest to the adhesive layer 22 is preferably lower than the melting point of the material disposed in the support layer 21 closest to the opposite side of the adhesive layer 22. The support layer 21 can, for example, be two layers: LDPE (low-density polyethylene) and PET (polyethylene terephthalate). By configuring it in this way, overflow of the adhesive layer 22 during lamination can be suppressed.

[0046] The resin contained in the support layer 21 may include fillers. The fillers may include at least one of organic and inorganic fillers. Organic fillers may include at least one selected from resin fillers, cellulose nanofibers, and pigments. Inorganic fillers may include at least one selected from oxides, hydroxides, carbides, nitrides, borides, silicides, and fluorides. The fillers may be spherical, polygonal, irregular, scaly, needle-like, or fibrous. The support layer 21 may be plate-like, sheet-like, woven, or non-woven.

[0047] <Analytical Carrier> The analytical carrier 1 includes a porous body 10 and a support 20. The support 20 may include an adhesive layer 22. The support 20 may also include an adhesive layer 22 and a support layer 21. The adhesive layer 22 of the support 20 can be bonded to the outer shell 14 of the porous body 10. The porous body 10 and the support 20 can be laminated by bonding the adhesive layer 22 to the outer shell 14 and sandwiching the adhesive layer 22 between the porous body 10 and the support layer 21. The adhesive layer 22 may include an adhesive portion 23 and an auxiliary portion 24. The support 20 may also include an adhesive layer 22 including the adhesive portion 23 and the auxiliary portion 24, and a support layer 21, and the adhesive portion 23, the auxiliary portion 24 and the support layer 21 are laminated sequentially. Alternatively, the porous body 10, the adhesive part 23, the auxiliary part 24 and the support layer 21 can be sequentially stacked by bonding the adhesive part 23 to the outer shell 14 and bonding the auxiliary part 24 to the support layer 21.

[0048] The adhesive layer 22 can be bonded to the outer shell 14 located on the surface of the porous body 10 opposite to the coating layer 17. In the porous body 10, the outer shell 14 can be bonded to the adhesive layer 22, and the coating layer 17 on the surface opposite to the outer shell 14 bonded to the adhesive layer 22 is exposed. In the analytical carrier 1, with the porous body 10 bonded to the support 20, the coating layer 17 is disposed on the opposite side of the support 20 and located on the outside of the porous body 10, while the hollow particles 13 and the outer shell 14 are located on the inside of the porous body 10 sandwiched between the coating layer 17 and the support 20. Thus, in the analytical carrier 1, the coating layer 17 is disposed on the outside of the porous body 10, the hollow particles 13 and the outer shell 14 are disposed on the inside of the porous body 10, and the hollow particles 13 and the outer shell 14 are covered by the coating layer 17 disposed on the surface of the analytical carrier 1. This prevents hollow particles 13 from peeling off from the porous material 10, the support 20, or the analytical carrier 1, thus improving operability.

[0049] The thickness of the analytical support 1 depends on the application, but can be, for example, 20 μm or more and 10 cm or less. By keeping the thickness of the analytical support 1 within this range, an analytical support 1 with good flexural strength can be provided. The thickness of the analytical support 1 can be 30 μm or more, 50 μm or more, 100 μm or more, or 150 μm or more. The thickness of the analytical support 1 can be less than 1000 μm, less than 300 μm, or less than 200 μm.

[0050] A porous material 10 can be provided on one surface of the support 20, i.e., the first surface, while the surface opposite to the first surface, i.e., the second surface, is exposed. This structure improves the situation in the analytical carrier 1 where re-attaching of the porous material 10 is necessary. Specifically, when manufacturing immunochromatographic test strips or detection kits, the attachment components constituting the immunochromatographic test strips or detection kits are attached to the support 20. However, when it is necessary to re-attach the attachment components after they have been peeled off the support 20 for purposes such as adjusting position, re-attaching can sometimes be difficult.

[0051] For example, when the porous material 10 is disposed on both sides of the aluminum substrate described later, if the adhesive member is peeled off after being attached to the porous material 10 on one side of the aluminum substrate, the porous material 10 may sometimes peel off from the aluminum substrate. In this case, the porous material 10 remains on the adhesive member, and therefore the adhesive force of the adhesive member may sometimes weaken. Furthermore, when the porous material 10 is disposed on one side of the aluminum substrate, if the adhesive member is peeled off after being attached to the side of the aluminum substrate where the porous material 10 is not disposed, the aluminum substrate may sometimes undergo plastic deformation. In this case, wrinkles may sometimes form on the porous material 10.

[0052] On the other hand, when a porous material 10 is provided on the first surface of the support 20 and the second surface of the support 20 is exposed, attaching the adhesive member to the support 20 can suppress the adverse effects caused by re-attachment as described above. Furthermore, the first and second surfaces can be the main surfaces. Here, the main surfaces refer to the surfaces perpendicular to the thickness direction of the support 20, and are the two surfaces with the largest and second largest areas among the six surfaces forming the support 20.

[0053] When the porous material 10 has absorbed water and is placed on a white reflectance standard calibrated by the measuring instrument, the L value is measured on the surface of the porous material 10 in the analytical carrier 1. a b L in the color system The value is above 80. By using this L Setting the value to 80 or higher can improve the discrimination during water absorption. This L The value can be above 85, above 90, or above 95. In this specification, L... a b L in the color system The value can be measured using a colorimeter with 45° annular illumination and vertical light reception according to JIS Z8722. Additionally, the analytical carrier 1 after the porous material 10 absorbs water can be obtained by absorbing pure water into the porous material 10. Furthermore, L is measured using this measuring instrument against a white reflectance standard calibrated with the measuring instrument. a b L in the color system The value is 97.1.

[0054] When the porous material 10 is dried and placed on a white reflectance standard calibrated by the measuring instrument, the L value of the surface of the porous material 10 in the analytical carrier 1 is measured using the measuring instrument. a b L in the color system The value can be above 80. By using this L... Setting the value to 80 or higher can also improve the discrimination during water absorption. This L The value can be above 85, above 90, or above 95. Furthermore, "dry" for porous material 10 refers to a state where porous material 10 has not absorbed water.

[0055] In the analytical carrier 1, it is preferable that the time required to draw water to a height of 4 cm by capillary action is 400 seconds or less. This allows for an analytical carrier 1 suitable for applications such as chromatography. This time can be 20 seconds or more, 40 seconds or more, or 50 seconds or more. Furthermore, this time can be 360 ​​seconds or less, 200 seconds or less, or 100 seconds or less. This time can be obtained, for example, by immersing the analytical carrier 1 in pure water at room temperature (30°C) with its plane perpendicular to the liquid surface, and measuring the time required for water to be drawn to a height of 4 cm by capillary action. Pure water refers to water with a resistivity of 10 kΩm measured at 30°C.

[0056] like Figure 16 and Figure 17 As shown, the analytical carrier 1 preferably does not experience peeling of the porous material 10 during the bending test. Furthermore, the analytical carrier 1 preferably leaves no wrinkles on the surface of the porous material 10 during the bending test. Specifically, in the bending test, 10g weights 230 are attached to both ends of the analytical carrier 1, which is cut to a length of 10mm × 100mm. Then, at the center of the long side of the analytical carrier 1, the analytical carrier 1 is placed on the stainless steel rod 240 with the porous material 10 connected to it, and lifted until the weights 230 at both ends of the analytical carrier 1 float up and held for 10 seconds. After holding for 10 seconds, the stainless steel rod 240 is returned to its original position, and the surface of the porous material 10 is visually observed and evaluated. The analytical carrier 1, which does not experience peeling of the porous material 10 during the bending test, is less prone to peeling when bent or reattached, thus facilitating the operation of the analytical carrier 1. Furthermore, the analytical carrier 1, which leaves no wrinkles on the surface of the porous material 10 during bending tests, is less prone to wrinkling when bent or re-attached, thus simplifying its operation. Additionally, peeling of the porous material 10 refers to the separation of the adhesive layer 22 from the porous material 10, specifically when the minimum width of the separated portion within the surface of the analytical carrier 1 is 2 mm or more and the maximum length is 5 mm or more. Wrinkles in the porous material 10 refer to millimeter-sized wrinkles that can be visually confirmed.

[0057] <Effects> As described above, the analytical carrier 1 of this embodiment includes a porous material 10 and a support 20 supporting the porous material 10 on one side surface. The porous material 10 includes a framework 11 formed by a plurality of hollow particles 13 and a plurality of pores 12 surrounded by the framework 11. The hollow particles 13 have a shell 14 containing an anodized film containing alumina and pores 15 surrounded by the shell 14. The framework 11 is formed continuously by the shells 14 of the plurality of hollow particles 13. The porosity of the porous material 10 is 50% by volume or more and less than 100% by volume. The average pore size of the porous material 10 is 0.1 μm or more and less than 20 μm. When the analytical carrier 1, after the porous material 10 has absorbed water, is placed on a white reflectance standard calibrated by the measuring instrument, the L value of the surface of the porous material 10 in the analytical carrier 1 is measured using the measuring instrument. a b L in the color system The value is above 80.

[0058] The outer shell 14, which includes an anodized film, has high transparency, and the porous body 10 becomes transparent when it absorbs water. As in Patent Document 1, in an aluminum component in which the porous body is supported by an aluminum substrate, when the porous body absorbs water, the gray color of the aluminum substrate supporting the porous body can be seen through the porous body, thereby making the aluminum component sometimes appear gray.

[0059] In the analytical carrier 1 of this embodiment, a support 20 is provided to support the porous material 10. Furthermore, when the analytical carrier 1, after the porous material 10 has absorbed water, is placed on a white reflectance standard calibrated with a measuring instrument, the L value of the surface of the porous material 10 in the analytical carrier 1 is measured using the measuring instrument. a b L in the color system The value is set to 80 or higher. Therefore, the analytical carrier 1 of this embodiment can improve the discriminative power when absorbing water. For example, when using a transparent resin as the support 20, the analytical carrier 1 can be evaluated on a white reflectance standard, which can improve the discriminative power when absorbing water.

[0060] [2. Immunochromatographic test strips] The immunochromatographic test strip includes an analytical carrier 1. The analytical carrier 1 allows for the analysis of the analyte while it is loaded with the analyte. The analytical carrier 1 improves the discriminative power upon water absorption, and therefore is suitable for use as an immunochromatographic test strip. Furthermore, the immunochromatographic test strip is also referred to as an immunochromatographic developing element, a lateral flow analysis test strip, or a lateral flow analysis developing element. Moreover, the analytical carrier 1 is preferably used in in vitro diagnostic medical products such as immunochromatographic test kits.

[0061] [3. Detection kit] Next, an example of using the test kit 50 with analytical carrier 1 will be described. The test kit 50 is sometimes also referred to as a diagnostic kit. Figure 2 As shown, the test kit 50 includes a test strip 60 with an analytical carrier 1. Specifically, the test strip 60 includes the analytical carrier 1, a sample supply section 62, and an absorption section 64. The analytical carrier 1 includes a determination section 66. The test kit 50 may also include a housing (not shown) for housing the test strip 60.

[0062] The sample supply unit 62 may, for example, contain a labeled antibody that specifically binds to the antigen being analyzed. A sample collected from a living organism or the like is supplied to the sample supply unit 62 and mixed with the labeled antibody to form a mixture. The mixture is spread to the determination unit 66 by the capillary effect of the analytical carrier 1, and the remaining sample is absorbed by the absorption unit 64.

[0063] The determination unit 66, for example, has a test line and a control line. A capture antibody that specifically binds to the analyte is immobilized at the test line, for example. If the sample contains the analyte, the labeled antibody is immobilized at the capture antibody site on the test line via the analyte. An antibody that specifically binds to the labeled antibody is immobilized at the control line, for example. If the mixture containing the sample and the labeled antibody spreads to the control line, the labeled antibody will bind to the antibody immobilized at the control line.

[0064] The labeled antibody contains a label, including colored particles, gold colloidal particles, or fluorescent beads, and an antibody that binds to the label to form a complex and specifically binds to the analyte. Therefore, in areas with high concentrations or densities of labeled antibodies, fluorescence can be detected due to the dense labeling. Thus, the test kit 50 can perform the following tests: a positive result if both the test line and control line are visible, and a negative result if only the control line is visible. The test line and control line can be visually confirmed when using colored particles or gold colloidal particles, and confirmed using a fluorescence detector such as a fluorescence scanner when using fluorescent beads.

[0065] Fluorescence can be detected, for example, by a fluorescence detector such as a fluorescence scanner. By using a fluorescence scanner, the analyte carrier 1, in a state carrying the analyte, can be irradiated with excitation light, and the fluorescence of the fluorescent beads excited by the excitation light can be detected.

[0066] The test kit 50 can be used for, for example: infectious disease detection; gene analysis; pregnancy testing; animal product testing; allergen detection such as food, animal, plant, metal, and house dust; etc.

[0067] Examples of analytes for the test kit 50 may include, for example, amino acids, peptides, proteins, genes, sugars, lipids, cells, or complexes thereof. More specifically, examples of analytes may include: peptides such as PCT (procalcitonin); proteins such as urinary albumin; hormones such as HCG (human chorionic gonadotropin) and LH (luteinizing hormone); HBs antigen, rotavirus antigen, adenovirus antigen, RSV (respiratory syncytial virus) antigen, influenza virus antigen, norovirus antigen, mumps virus antigen, cytomegalovirus antigen, herpes simplex virus antigen, varicella-zoster virus antigen, SARS (severe acute respiratory syndrome) antigen, HBs antibody, HCV (hepatitis C virus) antibody, HIV antibody, EBV antibody, etc. Antigens or antibodies against viral infectious diseases such as SARS, RSV, rubella, measles, enterovirus, dengue, and SARS; antigens or antibodies against bacterial infectious diseases such as pneumococcal antigen, mycoplasma antigen, group A streptococcal antigen, Legionella antigen, tuberculosis antigen, gonococcal antigen, tetanus antigen, mycoplasma antibody, Helicobacter pylori antibody, and tuberculosis antibody; antigens or antibodies against chlamydial infectious diseases such as chlamydia antigen; antigens or antibodies against spirochetal infectious diseases such as Treponema pallidum antibody; antigens or antibodies against protozoan diseases such as malaria antibody and Toxoplasma gondii antibody; etc.

[0068] Furthermore, the analytical carrier 1 can also be used, for example, as: a separation membrane for gases or liquids; a hygroscopic material; a water-absorbing material; an adsorbent material for adsorbing foreign matter such as pollen, particulate matter, bacteria, odor components, and heavy metals; a swab; a test strip for reagents such as concentrated sulfuric acid, urine tests, and pH tests; a sterilizing and disinfecting material; a reflective material; a standard white plate; a membrane for batteries and double-layer capacitors; a catalyst carrier; a reaction site for synthesis reactions; a heat-insulating material; and so on. Examples of the above-mentioned separation membranes include reverse osmosis membranes, ion exchange membranes, and gas separation membranes. Examples of the above-mentioned adsorbent materials include masks, filter membranes, and filter membranes.

[0069] [4. Manufacturing method of analytical carrier] Next, the manufacturing method of the analytical carrier 1 according to this embodiment will be described. The manufacturing method of the analytical carrier 1 according to this embodiment includes a sintering process, an anodizing process, a dissolution process, a lamination process, and a stripping process. In addition, the manufacturing method of the analytical carrier 1 may also include a hydration treatment process as needed. Each process will be described in detail below.

[0070] <Sintering Process> The sintering process involves sintering multiple aluminum metal particles 113 onto an aluminum substrate 105. Through this process, such as... Figure 3As shown, a sintered material 100 can be obtained comprising an aluminum substrate 105 and a sintered body 110 formed by sintering aluminum metal particles 113 and stacking them on the aluminum substrate 105. Figure 3 The example shown is a sintered body 110 formed by sintering aluminum metal particles 113 on one surface of an aluminum substrate 105. However, it is sufficient to sinter aluminum metal particles 113 on at least one surface of the aluminum substrate 105, or aluminum metal particles 113 can be sintered on both surfaces of the aluminum substrate 105. That is, the sintered body 110 may have aluminum metal particles 113 on one surface of the aluminum substrate 105, or aluminum metal particles 113 may be present on both surfaces of the aluminum substrate 105.

[0071] Aluminum metal particles 113 contain at least one type of aluminum selected from high-purity aluminum, pure aluminum, and aluminum alloys. Furthermore, the term "aluminum" will also be used hereinafter to refer to at least one type of aluminum selected from high-purity aluminum, pure aluminum, and aluminum alloys.

[0072] High-purity aluminum can have an aluminum content of 99.95% by mass or higher, or 99.99% by mass or higher, or 99.995% by mass or higher. Pure aluminum can have an aluminum content of 99.00% by mass or higher, or 99.50% by mass or higher, or 99.80% by mass or higher. Pure aluminum has an aluminum content of less than 99.95% by mass. Both high-purity and pure aluminum can contain elements other than aluminum (Al). The elements other than aluminum in pure aluminum can include one or more of the following elements: silicon (Si), iron (Fe), copper (Cu), manganese (Mn), magnesium (Mg), chromium (Cr), zirconium (Zn), titanium (Ti), vanadium (V), gallium (Ga), nickel (Ni), boron (B), and zirconium (Zr). Pure aluminum can also be an A1000 series alloy as specified in JIS H4000.

[0073] Aluminum alloys contain aluminum and elements other than aluminum. The elements other than aluminum in aluminum alloys can include one or more of the following elements: silicon (Si), iron (Fe), copper (Cu), manganese (Mn), magnesium (Mg), chromium (Cr), zirconium (Zn), titanium (Ti), vanadium (V), gallium (Ga), nickel (Ni), boron (B), and zirconium (Zr). The total content of the elements other than aluminum in aluminum alloys can be greater than 1% by mass. The total content of the elements other than aluminum in aluminum alloys can be less than 10% by mass or less than 5% by mass. The content of each element other than aluminum in aluminum alloys can be less than 10% by mass or less than 1% by mass. Aluminum alloys can also be 1000 series, 2000 series, 3000 series, 4000 series, 5000 series, 6000 series, 7000 series, or 8000 series alloys as specified in JIS H4000.

[0074] The average particle size of the multiple aluminum metal particles 113 is 0.1 μm or more and 20 μm or less. By setting the average particle size of the aluminum metal particles 113 to 0.1 μm or more, the strength of the porous material 10 is improved, and breakage during transport can be suppressed. By setting the average particle size of the aluminum metal particles 113 to 20 μm or less, the flow rate of the analytical carrier 1 can be improved. The average particle size of the aluminum metal particles 113 can be 1 μm or more, 3 μm or more, or 5 μm or more. The average particle size of the aluminum metal particles 113 can be 18 μm or less, 15 μm or less, or 10 μm or less. The average particle size of the multiple aluminum metal particles 113 before sintering is the D50 value obtained by measuring the particle size distribution on a volume basis using laser diffraction. The shape of the particles is not particularly limited and can be spherical, polygonal, irregular, scaly, or fibrous, etc.

[0075] Multiple aluminum metal particles 113 can be manufactured using known methods. For example, they can be manufactured by atomization, melt spinning, rotating disk method, rotating electrode method, or other rapid cooling and solidification methods. From an industrial production point of view, it is preferable to manufacture the multiple aluminum metal particles 113 by atomization, and more preferably by gas atomization. Specifically, the multiple aluminum metal particles 113 are preferably manufactured by atomizing molten metal.

[0076] The aluminum substrate 105 contains at least one type of aluminum selected from high-purity aluminum, pure aluminum, and aluminum alloys. The high-purity aluminum, pure aluminum, and aluminum alloys can be the materials described in the aluminum metal particles 113. The composition of aluminum contained in the aluminum metal particles 113 may be the same as or different from the composition of aluminum contained in the aluminum substrate 105.

[0077] The thickness of the aluminum substrate 105 exceeds 0 μm. While the thickness of the aluminum substrate 105 also depends on the application, it can be above 10 μm or above 20 μm. For example, the thickness of the aluminum substrate 105 can be below 1 mm, below 100 μm, below 10 μm, or below 1 μm.

[0078] Multiple aluminum metal particles 113 can be sintered after being disposed on at least one surface of the aluminum substrate 105. Aluminum powder containing multiple aluminum metal particles 113 can be disposed on at least one surface of the aluminum substrate 105, or a pressed powder obtained by pressing aluminum powder, or a liquid composition such as a slurry containing multiple aluminum metal particles 113 can be disposed. The liquid composition can be coated and disposed on the surface of the aluminum substrate 105 by known methods such as spraying, brushing, roller coating, air knife coating, rod coating, spin coating, dipping, and screen printing. The liquid composition only needs to be considered in terms of composition and coated to achieve the desired thickness of the porous body 10.

[0079] The surface of the aluminum substrate 105 can also be pretreated before the multiple aluminum metal particles 113 are disposed on the surface of the aluminum substrate 105. The pretreatment may include a process of roughening the surface of the aluminum substrate 105. The pretreatment is not particularly limited and may include cleaning, etching, or sandblasting.

[0080] In addition to multiple aluminum metal particles 113, aluminum powder or compositions may also contain, as needed, pore-forming materials, binders, sintering aids, surfactants, and solvents. These can all be made using known reagents.

[0081] The content of aluminum metal particles 113 in the composition is preferably 20% by mass or more, more preferably 30% by mass or more, and even more preferably 31% by mass or more. The content of aluminum metal particles 113 in the composition is preferably 70% by mass or less, more preferably 60% by mass or less, and even more preferably 57% by mass or less.

[0082] The pore-forming material is a material that promotes the formation of voids 12 within the sintered body 110. The pore-forming material can be, for example, particles containing a polymer. Preferably, the pore-forming material has low solubility in the solvents described later. The polymer can contain polysaccharides or resins. Polysaccharides can, for example, contain starch. Resins can contain polyolefins such as polyethylene or polypropylene.

[0083] The average particle size of the pore-forming material is preferably 0.1 μm or more and 20 μm or less. By setting the average particle size of the pore-forming material to 0.1 μm or more, the average fine pore size can be easily set to 0.1 μm or more. By setting the average particle size of the pore-forming material to 20 μm or less, the average fine pore size can be easily set to 20 μm or less. The average particle size of the pore-forming material can be 0.5 μm or more, or 1 μm or more. Furthermore, the average particle size of the pore-forming material can be 10 μm or less, or 8 μm or less. The average particle size of the pore-forming material is the D50 value obtained by measuring the particle size distribution using laser diffraction on a volume basis.

[0084] The content of the pore-forming material in the composition can be 5% by mass or more, 10% by mass or more, or 12% by mass or more. The content of the pore-forming material in the composition can be 30% by mass or less, 25% by mass or less, or 23% by mass or less. By having the content of the pore-forming material at or above the lower limit of the above range, the formation of voids 12 within the sintered body 110 is promoted, the filling rate of the sintered body 110 is reduced, thereby easily obtaining a sintered body 110 with the desired filling rate and a porous body 10 with the desired porosity. Furthermore, by having the content of the pore-forming material at or below the upper limit of the above range, excessive reduction in the filling rate of the sintered body 110 is prevented, and the reduction in the strength of the porous body 10 is easily suppressed.

[0085] The adhesive is preferably highly soluble in the solvents described later. The adhesive may include, for example, synthetic resins such as carboxyl-modified polyolefin resins, vinyl acetate resins, vinyl chloride resins, vinyl chloride-vinyl acetate copolymer resins, vinyl alcohol resins, butyral resins, fluorovinyl resins, acrylic resins, polyester resins, polyurethane resins, epoxy resins, urea resins, phenolic resins, acrylonitrile resins, nitrocellulose resins, paraffin wax, or polyethylene wax, or natural resins such as waxes, tar, animal glue, lacquer, rosin, or beeswax. The content of the adhesive in the composition may be 0.5% by mass or more, or 1% by mass or more. The content of the adhesive in the composition may be 30% by mass or less, or 20% by mass or less.

[0086] The solvent may include organic solvents such as water, ethanol, toluene, ketones, or esters. To allow the solvent to evaporate, the laminate obtained by coating the aluminum substrate 105 with the composition may be dried at a temperature above 20°C and below 300°C for 1 to 30 minutes, as needed.

[0087] The sintering temperature is not particularly limited, but is preferably 560°C or higher and 660°C or lower. Setting the sintering temperature to 560°C or higher increases the strength of the porous body 10. Setting the sintering temperature to 660°C or lower suppresses the melting of multiple aluminum metal particles 113. The sintering temperature can be 570°C or higher, or 580°C or higher. Furthermore, the sintering temperature can be 650°C or lower, or 620°C or lower.

[0088] The sintering time depends on factors such as the sintering temperature, and can range from approximately 5 to 24 hours. The sintering atmosphere is not particularly limited; it can be any of the following: a vacuum atmosphere, an inert gas atmosphere, an oxidizing gas atmosphere (atmosphere), or a reducing gas atmosphere. Among these atmospheres, a vacuum atmosphere or a reducing gas atmosphere is preferred. Furthermore, the sintering conditions can be any of the following pressure conditions: atmospheric pressure, reduced pressure, or pressurized pressure.

[0089] When the above composition contains a pore-forming material, it is preferable to heat the composition at a temperature of 200°C or higher and 500°C or lower before sintering. Heating the composition at 200°C or higher allows the pore-forming material to burn slowly, forming more uniformly dispersed voids 12 within the sintered body 110. Heating the composition at 500°C or lower suppresses oxidation of the aluminum metal particles 113 during heating, improving the strength of the porous body 10. The heating temperature can be 250°C or higher, or 280°C or higher. The heating temperature can be 460°C or lower, or 430°C or lower.

[0090] The heating time is preferably 5 hours or more and 20 hours or less. By setting the heating time to 5 hours or more, more uniformly dispersed voids 12 can be formed within the sintered body 110. By setting the heating time to 20 hours or less, the sintering of individual aluminum metal particles 113 with each other can be prevented, and more uniformly dispersed voids 12 can be formed within the sintered body 110. The heating time can be 7 hours or more or 15 hours or less. The sintering atmosphere can be any of a vacuum atmosphere, an inert gas atmosphere, or an oxidizing gas atmosphere. Furthermore, the sintering conditions can be any of atmospheric pressure, depressurization, or pressurization.

[0091] The sintering process may include a first sintering layer forming process to obtain a first sintering layer and a second sintering layer forming process to obtain a second sintering layer. In the first sintering layer forming process, a plurality of aluminum metal particles 113 are sintered to obtain a first sintering layer. As described above, the plurality of aluminum metal particles 113 may be sintered after being disposed on at least one surface of an aluminum substrate 105. In the second sintering layer forming process, a laminate obtained by disposing of the plurality of aluminum metal particles 113 on the surface of the first sintering layer may be sintered. In the first and second sintering layer forming processes, at least any one of the following may be different: average particle size, aspect ratio, composition of the constituent materials or composition, or type or average particle size of the pore-forming material used for the plurality of aluminum metal particles 113.

[0092] The filling rate of the sintered body 110 is 10% to 60% by volume. By setting the filling rate of the sintered body 110 to 10% by volume or more, a porous body 10 with a porosity below the upper limit of the desired range can be easily obtained after the anodizing and dissolution processes. This suppresses the detachment of aluminum metal particles 113 from the porous body 10 and improves the strength of the porous body 10. Furthermore, by setting the filling rate of the sintered body 110 to 60% by volume or less, a porous body 10 with a porosity above the lower limit of the desired range can be easily obtained after the anodizing and dissolution processes. This improves the absorption performance of the porous body 10. The filling rate can be 15% by volume or more, or 20% by volume or more. Furthermore, the filling rate can be 55% by volume or less, or 50% by volume or less. The filling rate, as described in the Examples section below, can be obtained by dividing the mass of the sintered body 110 obtained by subtracting the mass of the aluminum substrate 105 from the total mass of the sintered material by the mass of the sintered body 110 when the filling rate is assumed to be 100%.

[0093] <Hydration Treatment Process> The manufacturing method of the analysis carrier 1 in this embodiment can include a hydration treatment step before the anodizing process. The hydration treatment step is a process for efficiently forming an anodized film, wherein a hydration film is formed on the surface of the aluminum metal particles 113, so that during the subsequent anodizing process, the hydration film transforms into an oxide film. Furthermore, the hydration treatment step has the effect of causing stains and other contaminants present on the aluminum surface before anodizing to float to the surface and has a cleaning effect, thus suppressing the generation of uneven appearance when an anodized film is subsequently attached. The hydration treatment step is a process of heat-treating the sintered material 100 with hot water such as boiling water. The hot water can be pure water or an aqueous solution of phosphoric acid containing dissolved phosphoric acid. The concentration of the phosphoric acid aqueous solution can be, for example, 0.001 mL / L to 5 mL / L. This concentration of phosphoric acid aqueous solution can be obtained, for example, by preparing the desired concentration by using 85% by mass phosphoric acid aqueous solution with pure water. By adding phosphoric acid, the surface cleaning effect is improved. If the sintered body 110 is hydrated, a hydrated film based on aluminum hydroxide will be formed on the surface of the aluminum. As mentioned above, a hydrated film is sometimes included on the outer shell 14, but the outer shell 14 may also include a hydrated film.

[0094] <Anodizing Process> like Figure 4As shown, the anodizing process is a process of anodizing the sintered material 100 to form a shell 14 containing an anodized film on the surface of the aluminum metal particles 113. Furthermore, in the anodizing process, a coating layer 17 containing an anodized film containing aluminum oxide can be formed on the surface of the aluminum substrate 105 on which the aluminum metal particles 113 are stacked. In the anodizing process, for example, an anode on which the sintered material 100 is disposed and a cathode on which stainless steel (SUS) is disposed are immersed in an electrolyte for electrolytic treatment.

[0095] The electrolyte used in the anodizing process may contain at least one selected from citric acid, boric acid, phosphoric acid, sulfuric acid, oxalic acid, and their salts. By using an electrolyte containing citric acid, boric acid, phosphoric acid, or their salts for anodizing, a barrier-type anodized film can be formed. In the case of forming a barrier-type anodized film, compared to a porous type, anodized film of a certain thickness can be easily formed by controlling the voltage. By using an electrolyte containing sulfuric acid, oxalic acid, phosphoric acid, or their salts for anodizing, a porous type anodized film can be formed. In the case of forming a porous type anodized film, compared to a barrier type, anodized film can be formed using a lower voltage, thus reducing electricity costs. Furthermore, examples of salts include ammonium salts, sodium salts, potassium salts, and silicates. From the viewpoint of ease of acquisition, the salt preferably contains at least one of ammonium salts and sodium salts.

[0096] There are no particular limitations on the conditions for anodizing. For example, the electrolysis temperature can be above 0°C, 10°C, 20°C, 30°C, 40°C, or 50°C. The electrolysis temperature can be below 70°C, 60°C, or 50°C. The electrolysis voltage varies depending on the type of electrolyte, but can be above 0.1V or above 1V. Furthermore, the electrolysis voltage varies depending on the type of electrolyte, but can be below 500V or below 400V. Additionally, the electrolysis time can be above 0.1 minutes or above 1 minute. The electrolysis time can be below 60 minutes or below 20 minutes. Anodizing can be carried out in a single step or in multiple steps.

[0097] <Dissolving Process> like Figure 5As shown, the dissolution process is a process of dissolving the aluminum metal particles 113 surrounded by the outer shell 14. In the dissolution process, for example, it can be carried out by immersing the sintered material 100, to which the outer shell 14 is formed in the anodizing process, in a dissolving solution. In the dissolution process, high-purity aluminum, pure aluminum, and aluminum alloys contained in the aluminum metal particles 113 can dissolve inside the outer shell 14 and dissolve out to the outside of the outer shell 14. This creates voids 15 inside the outer shell 14. As a result, the outer shell 14 remains as a shell-like structure, thereby forming hollow particles 13. That is, hollow aluminum particles containing the outer shell 14 and the voids 15 surrounded by the outer shell 14 are formed. Furthermore, in the sintered body 110, a continuous series of outer shells 14 are formed on the outer surfaces of a plurality of adjacent aluminum metal particles 113. Therefore, through the anodizing process, the outer shell 14 derived from multiple aluminum metal particles 113 remains in a continuous form, and through the dissolution process, the interior of the aluminum metal particles 113 dissolves out, forming multiple cavities 15 in a connected and continuous manner inside the outer shell 14. As a result, a framework 11 is formed by the aggregation of multiple hollow particles 13.

[0098] The solution used to dissolve the aluminum metal particles 113 in the dissolution process contains at least one acid selected from phosphoric acid, sulfuric acid, nitric acid, and their salts, or at least one base selected from sodium hydroxide and potassium hydroxide. Such a solution exhibits excellent solubility for the aluminum metal particles 113. The solution preferably contains, for example, phosphoric acid. The salt contained in the aqueous solution may include at least one metal salt selected from aluminum, sodium, magnesium, calcium, and zinc.

[0099] When using phosphoric acid as the solvent, the concentration of phosphoric acid can be, for example, from 0.1 g / L to 1000 g / L. The dissolution temperature in the dissolution process can be, for example, from 50°C to 80°C. The dissolution time in the dissolution process can be from 1 minute to 60 minutes.

[0100] In the manufacturing method of the analytical carrier 1, a metal component (aluminum component) 120 with a porous body 10 stacked on an aluminum substrate 105 is formed by repeatedly performing anodizing and dissolution processes. By repeatedly performing the anodizing and dissolution processes, hollow particles 13 having a shell 14 containing an anodized film comprising aluminum oxide and cavities 15 surrounded by the shell 14 can be formed. Figure 5 The example shown is a metal member 120 with a porous body 10 formed on one side surface of an aluminum substrate 105. However, it is sufficient to form the porous body 10 on at least one side surface of the aluminum substrate 105, or the porous body 10 can be formed on both sides surface of the aluminum substrate 105. That is, the metal member 120 can have the porous body 10 stacked on one side surface of the aluminum substrate 105, or the porous body 10 can be formed on both sides surface of the aluminum substrate 105.

[0101] In the manufacturing method of the analytical carrier 1, the anodizing and dissolution processes can be repeated alternately, with the number of repetitions being more than 2 and less than 20. The number of repetitions of the anodizing and dissolution processes is affected by the voltage conditions of the anodizing process or the processing time of the dissolution process, and therefore is not particularly limited. However, by setting the number of repetitions to more than 2, the residual aluminum in the hollow particles 13 can be reduced, and the light transmittance of the porous material 10 can be improved. This provides an analytical carrier 1 with high visibility. Furthermore, even if the number of repetitions exceeds 20, the amount of residual aluminum is not easily reduced further, so by setting the number of repetitions to less than 20, the work efficiency can be improved. The number of repetitions can be, for example, more than 2, more than 3, more than 5, or more than 8. The number of repetitions of the anodizing and dissolution processes can be less than 15 or less.

[0102] In the dissolution process, the high-purity aluminum, pure aluminum, and aluminum alloy contained in the aluminum substrate 105 can be dissolved inside the aluminum substrate 105 and dissolved out to the outside of the aluminum substrate 105. This creates a space inside the aluminum substrate 105 corresponding to the dissolved portion. Here, the dissolution in the dissolution process proceeds from the aluminum metal particles 113 and towards the portion where the high-purity aluminum, pure aluminum, and aluminum alloy are present due to the sintering of the aluminum substrate 105 and the aluminum metal particles 113. Therefore, compared to the side where the coating layer 117, formed to cover the entire outer surface of the aluminum substrate 105, exists on the opposite side of the porous body 10, the side where the coating layer 17, formed to cover the inner surface of the aluminum substrate 105 and connected to the porous body 10 side via the connecting hole 18, is located is more easily dissolved. Thus, through the dissolution progress on the porous body 10 side, the aluminum substrate 105 on the same side as the porous body 10, near the boundary between the aluminum substrate 105 and the coating layer 17, dissolves and leaves a portion remaining, resulting in a connection between the aluminum substrate 105 and the coating layer 17 on the opposite side of the porous body 10. If the dissolution progresses too much, the aluminum substrate 105 and the coating layer 17 will peel off before the subsequent lamination process, reducing operability and making it difficult to fabricate the analytical carrier 1 supported by the support 20. Conversely, if the dissolution progresses too little, the separation of the porous body 10 from the aluminum substrate 105 becomes difficult in the subsequent peeling process. Therefore, it is preferable to dissolve until the porous body 10 side of the aluminum substrate 105 and the coating layer 17 are partially connected.

[0103] <Layering Process> like Figure 6As shown, the lamination process involves bonding the porous body 10 of the metal component 120 to the support 20, thereby forming a laminate 130 composed of an aluminum substrate 105, a porous body 10, and a support 20, which are sequentially stacked. In the lamination process, the adhesive layer 22 is bonded to the outer shell 14. By bonding the porous body 10 to the support 20, the support 20 can support the porous body 10. Furthermore, by sequentially stacking the aluminum substrate 105, the porous body 10, and the support 20, the aluminum substrate 105 can be easily peeled off in the subsequent peeling process. Figure 6 The example illustrates a laminate 130 in which the support 20 is bonded to a porous material 10 formed on one side surface of an aluminum substrate 105. However, it is sufficient to bond the support 20 to at least one side surface of the aluminum substrate 105, or the support 20 can be bonded to both sides of the aluminum substrate 105. That is, the metal member 120 can have the support 20 laminated on the porous material 10 formed on one side surface of the aluminum substrate 105, or it can have the support 20 laminated on the porous material 10 formed on both sides of the aluminum substrate 105.

[0104] With the support 20, before bonding the porous material 10, placed on a white reflectance standard calibrated by the measuring instrument, the L value of the surface of the support 20 for bonding the porous material 10 is measured using the measuring instrument. a b L in the color system The value is above 80. By using this L Setting the value to 80 or higher can improve the discrimination during water absorption. This L The value can be above 85, above 90, or above 95.

[0105] As described above, the support 20 may also include an adhesive layer 22 and a support layer 21. Furthermore, an adhesive that bonds the porous body 10 to the support layer 21 can be used for bonding. The adhesive can be cured to form the adhesive layer 22. The adhesive may be selected from at least one of solvent-evaporating adhesives, moisture-curing adhesives, heat-curing adhesives, curing agent-mixed adhesives, anaerobic-curing adhesives, UV-curing adhesives, hot-melt adhesives, pressure-sensitive adhesives, and rewetting adhesives. For example, the adhesive may contain an ethylene-vinyl acetate copolymer and bond the porous body 10 to the support layer 21 by hot melting.

[0106] Alternatively, for example, a single layer of thermoplastic resin can be used as the support 20, and heat can be applied to one side of the support 20 to melt it, thereby bonding the porous body 10 to the support 20. Alternatively, the support layer 21 can be omitted from the support 20, and the adhesive can be directly applied to the porous body 10 and cured, thereby forming the support 20 formed by the adhesive layer 22. The following describes the first to fourth layering processes corresponding to the structure of the support 20.

[0107] like Figure 7 As shown, the first lamination method can be applied to situations where the support 20a is formed by pre-laminating a support layer 21a made of thermoplastic resin and an adhesive layer 22a made of thermoplastic resin having a softening temperature lower than that of the support layer 21a. Such a support 20a is commercially available as a laminate. In the first lamination method, the support 20a is overlapped with the adhesive layer 22a facing the two outer sides of the metal member 120 and the support layer 21a facing outwards. In the overlapped state, heat is applied to the support 20a from the two outer sides where the support layer 21a is disposed, to a degree that softens the adhesive layer 22a while not softening the support layer 21a, thereby softening the adhesive layer 22a and bonding it to the metal member 120. In this way, by bonding the metal component 120 to the adhesive layer 22a on both sides of the metal component 120, and providing a support layer 21a on the outside of the adhesive layer 22a, a laminated body 130a consisting of the support layer 21a, the adhesive layer 22a, the metal component 120, the adhesive layer 22a, and the support layer 21a can be formed in sequence. When applying heat to the adhesive layer 22a for bonding, it is preferable to sandwich the overlapping metal component 120 and the support body 20a between at least one pair of rollers, and while applying heat and pressure to the metal component 120 and the support body 20a, rotate the rollers to move the metal component 120 and the support body 20a.

[0108] like Figure 8As shown, the second lamination method can be applied to situations where the support 20b is formed by bonding an adhesive layer 22b made of thermoplastic resin and a support layer 21b that can be bonded to the adhesive layer 22b. In the second lamination method, the adhesive layer 22b, the support layer 21b, and the support layer 21b are sequentially overlapped relative to the two outer sides of the metal member 120. In the overlapped state, by applying heat to the two outer sides of the support layer 21b, which is disposed thereon, to the extent that the adhesive layer 22b softens, the adhesive layer 22b can be softened and bonded to the metal member 120 and the support layer 21b. Thereby, by bonding and integrating the adhesive layer 22b and the support layer 21b, the support 20b is formed, and the metal member 120 is bonded to the support layer 21b via the adhesive layer 22b. Furthermore, by bonding the metal component 120 to the adhesive layer 22b on both sides of the metal component 120, and providing a support layer 21b on the outside of the adhesive layer 22b, a laminated body 130b consisting of the support layer 21b, adhesive layer 22b, metal component 120, adhesive layer 22b, and support layer 21b can be formed by sequentially stacking the support layer 21b, adhesive layer 22b, metal component 120, adhesive layer 22b, support layer 21b, and support layer 21b. When bonding by applying heat to the adhesive layer 22b, it is preferable to sandwich the overlapping metal component 120, adhesive layer 22b, adhesive layer 22b, support layer 21b, and support layer 21b between at least one pair of rollers, and while applying heat and pressure to the metal component 120, adhesive layer 22b, adhesive layer 22b, support layer 21b, and support layer 21b, the rollers are rotated to move the metal component 120, adhesive layer 22b, adhesive layer 22b, support layer 21b, and support layer 21b.

[0109] The third lamination method can be applied to cases where the support 20c consists solely of an adhesive layer 22c formed from thermoplastic resin. In this third lamination method, the adhesive layer 22c, the adhesive layer 22c, and the release liner 25 are sequentially overlapped relative to the two outer sides of the metal member 120. The release liner 25 can be suitably a sheet-like member that does not soften when heated, does not adhere to the adhesive layer 22c, and can transfer heat to the adhesive layer 22c. For example, a sheet (release paper) formed from paper can be used as the release liner 25. In the overlapped state, by applying heat to the two outer sides of the release liner 25 to soften the adhesive layer 22, the adhesive layer 22 can be softened, and the adhesive layer 22 is bonded to the metal member 120 in the same manner as in the first and second lamination methods. After bonding, as... Figure 9As shown, the self-adhesive layer 22 peels off the release body 25. This allows the metal member 120 to be bonded to the adhesive layer 22 on both sides, forming a laminate 130c consisting of the adhesive layer 22, the metal member 120, and the adhesive layer 22 stacked sequentially. When bonding by applying heat to the adhesive layer 22, it is preferable to sandwich the overlapping metal member 120, the adhesive layer 22, and the release body 25 between at least one pair of rollers, applying heat and pressure to the metal member 120, the adhesive layer 22, and the release body 25 while rotating the rollers to move the metal member 120, the adhesive layer 22, and the release body 25.

[0110] The fourth lamination method can be applied to situations where the support 20d is formed by bonding an adhesive layer 22d (formed by an adhesive) and a support layer 21d that can be bonded to the adhesive layer 22d. For example... Figure 10 As shown, in the fourth lamination method, firstly, adhesive is applied to one side of each of the support layers 21d to form adhesive layers 22d. Next, with the adhesive layers 22d facing the metal member 120, the support layers 21d, on which the adhesive layers 22d are formed, are overlapped on both outer sides of the metal member 120. In the overlapped state, by applying a force to the two outer sides of the support layers 21d to bond the adhesive layers 22d to the metal member 120 and the support layers 21d, the adhesive layers 22d are bonded to the metal member 120 and the support layers 21d. Thus, by bonding and integrating the adhesive layers 22d and the support layers 21d, a support body 20d is formed, and the metal member 120 is bonded to the support layers 21d via the adhesive layers 22d. Furthermore, by bonding the metal member 120 to the adhesive layer 22d on both sides of the metal member 120, and providing a support layer 21d on the outside of the adhesive layer 22b, a laminated body 130d consisting of the support layer 21d, the adhesive layer 22d, the metal member 120, the adhesive layer 22d, and the support layer 21d can be formed in sequence. When applying force to the adhesive layer 22b for bonding, it is preferable to sandwich the overlapping metal member 120, the adhesive layer 22d, the adhesive layer 22d and the support layer 21d between at least one pair of rollers, and while applying pressure to the metal member 120, the adhesive layer 22d and the support layer 21d, and the support layer 21d, rotate the rollers to move the metal member 120, the adhesive layer 22d, the adhesive layer 22d and the support layer 21d.

[0111] <Stripping Process> like Figure 11As shown, the peeling process involves dissolving the aluminum substrate 105 and peeling it off from the laminate 130 to form the analytical carrier 1. By peeling the aluminum substrate 105 off from the laminate 130, the porous material 10 can be placed in a visible state. Figure 11 The example illustrates a laminate 130 in which an aluminum substrate 105 and a coating layer 117 are peeled off. However, it is possible to form an analytical carrier 1 from at least one surface of the laminate 130 by peeling off the aluminum substrate 105, or from both surfaces of the laminate 130. That is, the laminate 130 provided for the peeling process can have a support 20 laminated on a porous material 10 formed on one surface of the aluminum substrate 105, or the support 20 can be laminated on a porous material 10 formed on both surfaces of the aluminum substrate 105.

[0112] In the stripping process, a stripping solution can be used to dissolve the aluminum substrate 105. In the stripping process, more than 10%, more than 20%, more than 30%, more than 40%, more than 50%, more than 60%, more than 70%, more than 80%, or more than 90% of the aluminum substrate 105 can be dissolved.

[0113] In the peeling process, an acidic or alkaline solution can be used to dissolve the aluminum substrate 105, causing the coating layer 117 formed on the surface of the aluminum substrate 105 opposite to the porous body 10 to separate from the porous body 10. This allows the fabrication of an analytical carrier 1 comprising a porous body 10 including the coating layer 17 and a support 20. Alternatively, the aluminum substrate 105 can be dissolved, allowing the coating layer 117 to peel off naturally from the porous body 10. Furthermore, the aluminum substrate 105 can be partially dissolved, and peeled off manually or mechanically from the laminate 130. Alternatively, the aluminum substrate 105 can be peeled off from the laminate 130 without dissolving it after the lamination process, and the peeling can be performed manually or mechanically from the laminate 130.

[0114] The stripping solution used in the stripping process may contain at least one alkali selected from sodium hydroxide and potassium hydroxide. Stripping solutions containing these alkalis have low viscosity and easily penetrate deep into the interior of the porous material 10. Therefore, by using such a stripping solution, the stripping of the aluminum substrate 105 can be promoted. The stripping solution can be an aqueous solution, for example, an aqueous solution containing sodium hydroxide.

[0115] The stripping solution used in the stripping process may contain at least one organic acid selected from citric acid and gluconic acid. Stripping solutions containing these organic acids can cause the dissolved aluminum to form complexes within the stripping solution. Therefore, by using these stripping solutions, aluminum precipitation can be inhibited, and the stability of the stripping solution can be improved. The stripping solution may also contain the aforementioned alkalis and organic acids. For example, the stripping solution may also contain sodium hydroxide and citric acid.

[0116] The stripping solution used in the stripping process may contain at least one acid selected from phosphoric acid, sulfuric acid, and nitric acid. Stripping solutions containing these acids can also strip the aluminum substrate 105. For example, the stripping solution may contain phosphoric acid, or it may contain both phosphoric acid and sulfuric acid.

[0117] The temperature of the stripping solution in the stripping process can be above 30°C and below 70°C. By setting the temperature of the stripping solution above 30°C, the stripping of the aluminum substrate 105 can be promoted, shortening the time required for the stripping process. By setting the temperature of the stripping solution below 70°C, excessive bubble generation can be suppressed. Therefore, damage to the porous material 10 can be prevented during the stripping process.

[0118] In the stripping process, only one stripping solution can be used and the aluminum substrate 105 can be stripped in only one stage. Alternatively, the aluminum substrate 105 can be stripped in multiple stages. When stripping the aluminum substrate 105 in multiple stages, the same type of stripping solution can be used in each stage, or different types of stripping solutions can be used. When stripping the aluminum substrate 105 in multiple stages, the stripping conditions, such as stripping temperature and stripping time, can be the same or different in each stage. Furthermore, after treating the aluminum substrate 105 with the stripping solution, it can be subjected to neutralization treatment, washing, drying, etc., using a solution containing phosphoric acid.

[0119] <Effects> As described above, the manufacturing method of the analytical carrier 1 in this embodiment is a method for manufacturing an analytical carrier 1 comprising a porous body 10 and a support 20 supporting the porous body 10 on one side surface. The porous body 10 includes a framework 11 formed by a plurality of hollow particles 13 and a plurality of voids 12 surrounded by the framework 11. The hollow particles 13 have a shell 14 containing an anodized film containing alumina and voids 15 surrounded by the shell 14. The framework 11 is formed continuously by the shells 14 of the plurality of hollow particles 13. The manufacturing method includes a sintering process in which a plurality of aluminum metal particles 113 are sintered on an aluminum substrate 105 to obtain a sintered material 100 comprising an aluminum substrate 105 and a sintered body 110 formed by sintering aluminum metal particles 113 on the aluminum substrate 105. The manufacturing method includes an anodizing step, wherein the anodizing step is performed on the sintered material 100 to form a shell 14 containing an anodized film on the surface of the aluminum metal particles 113. The manufacturing method includes a dissolution step, wherein the aluminum metal particles 113 surrounded by the shell 14 are dissolved. In the manufacturing method, by repeatedly performing the anodizing and dissolution steps, a metal component 120 with a porous body 10 stacked on an aluminum substrate 105 is formed. The manufacturing method includes a lamination step, wherein the porous body 10 of the metal component 120 is bonded to a support 20 to form a laminate 130 consisting of the aluminum substrate 105, the porous body 10, and the support 20 stacked sequentially. The manufacturing method includes a peeling step, wherein the aluminum substrate 105 is dissolved and peeled off from the laminate 130. The aluminum metal particles 113 contain at least one type of aluminum selected from high-purity aluminum, pure aluminum, and aluminum alloys. The aluminum substrate 105 contains at least one type of aluminum selected from high-purity aluminum, pure aluminum, and aluminum alloys. The average particle size of the plurality of aluminum metal particles 113 is 0.1 μm or more and 20 μm or less. The filling rate of the sintered body 110 is 10% by volume or more and 60% by volume or less. With the support 20, before bonding the porous body 10, placed on a white reflectance standard calibrated by the measuring instrument, the L value of the surface of the support 20 for bonding the porous body 10 is measured using the measuring instrument. a b L in the color system The value is above 80. Based on this manufacturing method, the analytical carrier 1 described above can be manufactured. Example

[0120] The present embodiment will be described in more detail below through examples and comparative examples, but the present embodiment is not limited to these.

[0121] [Example 1] (Slurry preparation) First, a slurry was prepared by uniformly dispersing 24 parts by weight of aluminum metal particles, 26 parts by weight of pore-forming material, and 1 part by weight of binder in 49 parts by weight of solvent. The aluminum metal particles (AHZL58FN manufactured by Toyo Aluminum Co., Ltd.) were pure aluminum (JIS A1080) with a purity of 99.80% by weight or higher. The aluminum metal particles were approximately spherical with an average particle size of 3 μm. As the pore-forming material, starch (NIKKALYCO AS-500S, a registered trademark manufactured by NIKKALYCO Co., Ltd.) with an average particle size of 7 μm was used. Ethyl cellulose resin was used as the binder. Butyl acetate was used as the solvent.

[0122] (sintering) A slurry was applied to one side surface of an aluminum substrate (aluminum base material) with a thickness of 30 μm, using a connector (registered trademark) from Hirano TECSEED Co., Ltd. The slurry was dried at 100°C for 1.5 minutes, heated at 350°C for 5 hours in air, and further sintered at 620°C–640°C for 10 hours in argon atmosphere. This produced a sintered material with a sintered body disposed on an aluminum substrate.

[0123] (Hydration treatment) Next, the sintered material was cleaned for 3 minutes at 50°C using a 5 g / L oxalic acid aqueous solution. Then, the sintered material was immersed in a 0.5 mL / L aqueous solution of 85% phosphoric acid prepared with pure water at 85°C for 3 minutes for hydration treatment. Finally, the hydrated sintered material was cleaned with pure water at room temperature (30°C) for 1 minute.

[0124] (First anodizing) The sintered material after hydration treatment undergoes a first anodizing process to form an aluminum oxide shell on the surface of the aluminum metal particles. Specifically, the sintered material at the anode and the stainless steel (SUS) at the cathode are immersed in an electrolyte prepared at 50°C with 98% citric acid in pure water at a concentration of 0.1 g / L. Then, an anode is applied at 50 mA / cm². 2 The current is passed through until the voltage reaches 1000V, and the voltage is maintained at 450V for 3 minutes to perform anodizing. The sintered material after the first anodization is then rinsed with pure water for 0.5 minutes.

[0125] (First dissolution treatment) The first dissolution treatment is performed, in which the sintered material after the first anodization is immersed in a 500 mL / L (718 g / L) aqueous solution of 85% phosphoric acid prepared with pure water at 70°C for 3.5 minutes to further dissolve the aluminum metal. The sintered material after the first dissolution treatment is then rinsed with pure water for 1 minute.

[0126] (Second anodizing) The sintered material after the first dissolution treatment undergoes a second anodizing to further form an aluminum oxide shell on the surface of the aluminum metal particles. Specifically, the sintered material at the anode and the stainless steel (SUS) at the cathode are immersed in an electrolyte at 50°C, prepared by dissolving 98% citric acid in pure water to a concentration of 1 g / L and 95% triammonium citrate to a concentration of 0.1 g / L. Then, an electrode is applied at 50 mA / cm². 2 Flow current until the voltage reaches 50V, maintain the voltage at 50V for 3 minutes, and perform anodizing treatment. Rinse the sintered material after the second anodizing with pure water for 0.5 minutes.

[0127] (Second dissolution treatment) A second dissolution treatment is performed, in which the sintered material after the second anodization is immersed in a 500 mL / L (718 g / L) aqueous solution of 85% phosphoric acid prepared with pure water at 70°C for 2.5 minutes to further dissolve the aluminum metal. The sintered material after the second dissolution treatment is then rinsed with pure water for 1 minute.

[0128] (Third anodizing) The sintered material after the second dissolution treatment undergoes a third anodizing process to further form an aluminum oxide shell on the surface of the aluminum metal particles. Specifically, the sintered material at the anode and the stainless steel (SUS) at the cathode are immersed in an electrolyte at 50°C, prepared by dissolving 98% citric acid in pure water to a concentration of 1 g / L and 95% triammonium citrate to a concentration of 0.1 g / L. Then, an electrode is applied at 50 mA / cm². 2 The current is passed through until the voltage reaches 50V, and the voltage is maintained at 50V for 3 minutes to perform anodizing. The sintered material after the third anodizing is then rinsed with pure water for 0.5 minutes.

[0129] (Third dissolution treatment) A third dissolution treatment is performed, in which the sintered material after the third anodizing is immersed in a 30 mL / L (43 g / L) aqueous solution of 85% phosphoric acid prepared with pure water at 70°C for 30 minutes to further dissolve the aluminum metal. The sintered material after the third dissolution treatment is then rinsed with pure water for 1 minute.

[0130] (Drying process) The sintered material after the third melting treatment was dried at 200°C for 2 minutes to produce the aluminum component in this example.

[0131] (Layering) The stacking process is implemented using stacking method 1. Stacking method 1 is described below.

[0132] (Cascading Method 1) Using a transparent laminated film as a support, the laminated film, acting as the support, is overlapped on both sides of the aluminum component prepared as described above. The aluminum component and the laminated film are then stacked in this state to create a laminated body with laminated films stacked on both sides of the aluminum component. As the laminated film, a laminated film (PET / LDPE / EVA, 100μm thick, GLOSS) manufactured by Fellowes is used. An EVA film is used as the adhesive layer, and a PET / LDPE film is used as the support layer. As the laminator, a Proteus A3 manufactured by Fellowes is used to laminate the aluminum component onto the laminated film at a lamination temperature of 90°C and a lamination speed of approximately 1.4cm / s. The laminated body with the aluminum component stacked on the laminated film is then cut into 25mm wide pieces. Lamination method 1 corresponds to the first lamination method described above.

[0133] (Stripping process) The stripping process is carried out using stripping condition 2. Stripping condition 2 is described below.

[0134] (Stripping condition 2) The 25 mm wide laminate obtained above was immersed in an aqueous solution prepared with pure water at 40°C for 30 minutes. This treatment peeled off the aluminum substrate from the laminate. Then, it was neutralized by immersion in an aqueous solution prepared with pure water at 40 mL / L of 85% phosphoric acid for 5 minutes at 70°C.

[0135] (Drying process) The analytical carrier for this example was prepared by washing the laminate with the aluminum substrate removed with pure water for 1 minute and drying it at 50°C for 10 minutes.

[0136] [Examples 2 to 26] Except for the conditions set in Tables 1 to 7, the analytical carrier was prepared in the same manner as in Example 1. Furthermore, the lamination methods 2 to 4 described in Table 7 are as follows. Additionally, the peeling conditions 1 and 3 described in Table 2 are as follows. Furthermore, the following aluminum metal particles were used.

[0137] (Cascading Method 2) Using the adhesive and support layers described in Table 1, the adhesive and support layers are overlapped on both sides of the aluminum component fabricated as described above. In this state, the adhesive layer, aluminum component, and support layer are stacked to create a laminated body with a support formed by the adhesive and support layers stacked on both sides of the aluminum component. Using the same laminator as in Example 1, the lamination is performed at a lamination temperature of 140°C and a lamination speed of approximately 0.7 cm / s. The laminated body is then cut into 25 mm wide pieces. Lamination method 2 corresponds to the second lamination method described above.

[0138] (Cascading Method 3) Using the adhesive layers described in Table 1, adhesive layers and release paper are overlapped on both sides of the aluminum component prepared as described above. In this state, the adhesive layers are laminated with the aluminum component, and the release paper is peeled off, thereby creating a laminate with supports formed by adhesive layers laminated on both sides of the aluminum component. Using the same laminator as in Example 1, lamination is performed at a lamination temperature of 140°C and a lamination speed of approximately 0.7 cm / s. The laminate is then cut into pieces 25 mm wide. Lamination method 3 corresponds to the third lamination method described above.

[0139] (Cascading Method 4) Using the adhesive layer and support layer described in Table 1, an adhesive layer is formed by applying adhesive to both sides of the support layer. Then, on both sides of the aluminum component fabricated as described above, a support layer with the adhesive layer is formed by overlapping the support layer with the aluminum component facing towards it. In this state, the adhesive layer is bonded to the aluminum component and the support layer, thus creating a laminate with a support body formed by the adhesive layer and support layer stacked on both sides of the aluminum component. As the adhesive, Aronalpha EXTRA impact-resistant (cyanoacrylate-based adhesive) manufactured by Toa Synthetic Co., Ltd. is used. The laminate is cut into 25mm wide pieces. Lamination method 4 corresponds to the fourth lamination method described above.

[0140] (Stripping condition 1) The 25 mm wide laminate obtained above is immersed in an aqueous solution prepared by mixing pure water with 300 mL / L of 98% sulfuric acid and 300 mL / L of 85% phosphoric acid, at 70°C for 3 minutes. Then, the 25 mm wide laminate is immersed in an aqueous solution prepared by mixing pure water with 500 mL / L of 85% phosphoric acid, at 70°C for 5 minutes. This treatment removes the aluminum substrate from the laminate.

[0141] (Stripping condition 3) For the 25mm wide laminate obtained above, hold the ends of the analytical carrier and the aluminum substrate by hand, and stretch them at a speed of 5mm / s in opposite directions to the normal direction of the laminate, thereby removing the aluminum substrate from the laminate.

[0142] (Aluminum metal particles) Average particle size 1.8μm: AHU091 manufactured by Toyo Aluminum Co., Ltd. Average particle size 5μm: AHZL58CN manufactured by Toyo Aluminum Co., Ltd. Average particle size 9μm: AHZL560F manufactured by Toyo Aluminum Co., Ltd. Average particle size 15μm: AHZL530C manufactured by Toyo Aluminum Co., Ltd.

[0143] [Comparative Example 1] Except for the conditions set in Tables 1 to 7, the analytical carrier was prepared in the same manner as in Example 1, except that the aluminum components that have undergone drying were not subjected to the subsequent processing (lamination process).

[0144] [Comparative Examples 2-3] Except for the conditions set in Tables 1 to 7, the analytical carrier was prepared in the same manner as in Example 1.

[0145] [Reference Example] Cytiva nitrocellulose membrane AE99 was used as the analytical carrier.

[0146] [evaluate] The following evaluation was performed on the analysis carriers for each case.

[0147] (Fill rate) The filling rate of the sintered body was obtained by measuring the sintered material before hydration treatment in the following order. • Cut a 100cm² area from the sintered material 2 The sample was measured. The following describes the measurement and calculation of the sample. (In addition, the projected area in this specification refers to the horizontal projected area of ​​the sintered material or sintered body when viewed from above in the same direction as the thickness direction.) • Determine the mass Ma (g) of the sintered material in which the sintered body and the aluminum substrate are combined. • The overall thickness Wa (cm) of the sintered body was measured using a micrometer. • The thickness Wb (cm) of the aluminum substrate was measured using a micrometer. • Calculate the mass Mb (g) of the aluminum substrate using Mb = density of aluminum substrate × Wb × 100. In this embodiment, 2.7 g / cm 3 Calculate the material density of the aluminum substrate and the sintered body, which are made of aluminum. The mass Mc(g) of the sintered body is obtained by using Mc = Ma - Mb. The mass Md (g) of the sintered body when the filling rate is assumed to be 100% is obtained by using Md = material density of the sintered body × (Wa-Wb) × projected area of ​​the sintered body = 2.7 × (Wa-Wb) × 100. • The filling rate of the sintered body in the test sample is obtained by calculating the filling rate (%) = (Mc / Md) × 100.

[0148] (Porosity of porous materials) The porosity of a porous material is obtained by dividing the cumulative pore volume of the porous material by the volume of the porous material. The cumulative pore volume of the porous material is obtained by accumulating the pore volume of pores with a diameter of 0.1 μm or more and 100 μm or less, as determined by mercury infiltration.

[0149] (Cross-section observation) An analytical support was frozen by immersing it in liquid nitrogen. The frozen support was then bent to break, and platinum was deposited onto the fracture surface at a thickness of approximately 5 nm to obtain a sample for cross-sectional observation. A cross-section of the sample was observed using a Carl Zeiss ULTRA plus scanning electron microscope in a plane orthogonal to the interface between the porous material and the adhesive layer, thereby obtaining a cross-sectional SEM image. The cross-sectional image of Example 5 is shown below. Figure 12 In the middle. Additionally, in Figure 12 In the symbol 10, the symbol 20 represents the support, and the symbol 80 represents the fragments of the porous material 10 that break apart and scatter onto the support when it breaks.

[0150] (Thickness of the outer shell) The thickness of the shell was measured using a cross-sectional SEM image obtained through the aforementioned cross-sectional observation method. The shell thickness was obtained by measuring the thickness of the shell of 10 hollow particles and calculating the average value.

[0151] (Thickness of porous material) The thickness of the porous body was measured using a cross-sectional SEM image obtained through the aforementioned cross-sectional observation method. Within a 50 μm wide area parallel to the interface between the porous body and the adhesive layer, the thickness at the location where the thickness of the material constituting the porous body reaches its maximum in the thickness direction was measured, and this thickness was taken as the porous body thickness. The thickness of the porous body was obtained by measuring the thickness of the porous body at five points at five different locations and calculating the average value.

[0152] (Thickness of the adhesive layer) The thickness of the adhesive layer was measured using a cross-sectional SEM image obtained by observation using the above-described cross-sectional observation method. The thickness of the adhesive layer was measured at the location where the thickness of the material constituting the adhesive layer reaches its maximum within a 50 μm wide range parallel to the bonding interface between the porous material and the adhesive layer. This thickness was used as the adhesive layer thickness. The thickness of the adhesive layer was obtained by measuring the thickness of the adhesive layer at five points at five different locations and calculating the average value. In the table, the support thickness and support layer thickness (substrate thickness) refer to the support thickness in Examples 1-22, and the support layer thickness (substrate thickness) in Examples 23-26 and Comparative Examples 1-3.

[0153] (Thickness of the bonded portion) The thickness of the adhesive portion was measured using a cross-sectional SEM image obtained through the aforementioned cross-sectional observation method. Within a 50 μm wide area parallel to the bonding interface between the porous material and the adhesive layer, two lines were drawn: one passing through the portion of the porous material closest to the adhesive layer (lowest part) and parallel to the bonding interface direction; the other passing through the portion of the adhesive layer closest to the porous material (upper part) and parallel to the bonding interface direction between the porous material 10 and the adhesive layer 22. The interval between these two lines was measured and used as the thickness of the adhesive portion 23. The thickness of the adhesive portion was obtained by measuring the thickness of the adhesive portion at five points at five different locations and calculating the average value.

[0154] (Surface observation) A sample for surface observation was obtained by depositing a platinum film of approximately 5 nm thickness onto the surface of an analytical carrier. The surface of the porous bulk side of the sample, i.e., the surface of the coated layer, was observed using a Carl Zeiss ULTRA plus scanning electron microscope, resulting in surface observation photographs (surface SEM images). The surface observation photograph of Example 5 is shown below. Figure 13 middle.

[0155] (Cumulative pore surface area of ​​porous materials) The cumulative pore surface area of ​​the porous material is obtained by converting the pore volume of pores with a diameter of 0.1 μm or more and 100 μm or less, determined by mercury infiltration, into surface area for each pore diameter and then summing the results. Furthermore, in Figure 14 The diagram shows the relationship between the pore size and the differential pore surface area for Example 5 and the reference example. Furthermore, in... Figure 15 The figure shows a graph illustrating the relationship between the pore diameter and the cumulative pore surface area for Example 5 and the reference example.

[0156] (Average pore size of porous material (4V / A)) The average pore size (4V / A) of the porous material can be calculated using the following mathematical formula. The average pore diameter of a porous material = 4 × (cumulative pore volume of the porous material) / (cumulative pore surface area of ​​the porous material) The cumulative pore volume and cumulative pore surface area of ​​the porous material were obtained as described above.

[0157] (Cumulative pore surface area ratio) Calculate the ratio of the cumulative pore surface area of ​​pores with a pore size of 0.1 μm or more but less than 1 μm to the cumulative pore surface area of ​​pores with a pore size of 1 μm or more but less than 10 μm. Additionally, as... Figure 15 As shown, the cumulative pore surface area of ​​pores with a pore size of 1 μm or more and less than 10 μm is obtained by accumulating the pore surface areas of pores with a pore size of 1 μm or more and less than 10 μm. Similarly, the cumulative pore surface area of ​​pores with a pore size of 0.1 μm or more and less than 1 μm is obtained by accumulating the pore surface areas of pores with a pore size of 0.1 μm or more and less than 1 μm. The cumulative pore surface area of ​​the porous material is determined as described above using the mercury infiltration method.

[0158] (The ratio of metallic aluminum to the components that make up porous materials containing aluminum) As described below, the content of metallic aluminum relative to the aluminum-containing components constituting the porous material is determined, and the ratio of metallic aluminum to the aluminum-containing components constituting the porous material is calculated. 1. Use a scraper made of SUS to scrape the analytical carrier to remove the porous material. 2. Take 2.0g of the self-scraped sample. 3. For the measured sample, the aluminum content (mass %) relative to the components constituting the porous body containing aluminum was determined according to the ICP emission spectrometry method for the decomposition and separation of metallic aluminum in JIS G2404:2022. 4. Using the following formula, calculate the ratio of metallic aluminum to the components of the porous material containing aluminum, based on the percentage (mass%) of metallic aluminum relative to the components containing aluminum. The ratio of metallic aluminum to the components constituting porous materials containing aluminum is calculated as follows: (Metallic aluminum content relative to the components constituting porous materials containing aluminum) / (100 - (Metallic aluminum content relative to the components constituting porous materials containing aluminum)).

[0159] (Arithmetic mean roughness Sa) The arithmetic mean roughness Sa of the porous material side surface of the analytical supports in the Examples and Comparative Examples was measured according to ISO 25178. The arithmetic mean roughness Sa was measured using a Contour GT-I three-dimensional white interferometer microscope manufactured by Bruker AXS Co., Ltd., under conditions of a measurement range of 60 μm × 79 μm, an objective lens magnification of 115x, and an internal lens magnification of 1x. For the analytical supports of Examples 1 to 26, Comparative Examples 2 and 3, the surface of the porous material side supported by the support structure of the analytical support was observed. For Comparative Example 1, the surface of the porous material side supported by the aluminum substrate was observed. For Reference Example 1, the surface of the nitrocellulose membrane supported by the backing sheet was observed. For each sample, a 60 μm × 60 μm image was acquired, and the surface roughness Sa (μm) was calculated. The average value of the images from a total of 5 fields of view was calculated to obtain the surface roughness Sa.

[0160] (Flow rate) The flow rate was determined as follows: First, the analytical carrier was immersed in pure water with its plane perpendicular to the liquid surface. Then, the time from immersion of the analytical carrier in the pure water until water was drawn up to a height of 4 cm from the liquid surface by capillary action was measured and evaluated as the flow rate.

[0161] (L when dry) value) Determine the following L The value, i.e., L, is the value when the surface of the porous material in the analytical carrier is measured using the measuring instrument after the porous material has been dried and placed on a white reflectance standard calibrated by the measuring instrument. a b L in the color system Value. L The values ​​were measured using a colorimeter (CR-331C, manufactured by Konica Minolta Co., Ltd., Japan) with 45° annular illumination and vertical light reception according to JIS Z8722. The white reflectance standard was measured using a white calibration plate CR-A46 (Y: 92.7, x: 0.3129, y: 0.3189) manufactured by Minolta.

[0162] (L when absorbing water) value) Determine the following L The value, i.e., L, is the value when the surface of the porous material in the analytical carrier is measured using the measuring instrument after the porous material has absorbed water and is placed on a white reflectance standard calibrated with the measuring instrument. a b L in the color system Specifically, in a shallow dish containing 5 mm of pure water, an analytical carrier cut to 3 cm × 3 cm is placed vertically relative to the water surface in the thickness direction on the bottom of the dish. After water is drawn up to the top of the porous material in the analytical carrier, the analytical carrier is removed from the dish and placed on a white reflective standard with the porous surface of the analytical carrier facing upwards. With the porous material of the analytical carrier having absorbed pure water, the surface of the analytical carrier with the porous material is measured using a colorimeter (CR-331c manufactured by Konica Minolta) in the same manner as described above.

[0163] (L of the support) value) Determine the following L The value, i.e., L, is measured by the measuring instrument on the surface of the support for bonding the porous material, when the support is placed on a white reflectance standard calibrated before bonding the porous material. a b L in the color system Values. For the support, measurements were performed in the same manner as described above using a colorimeter (CR-331c manufactured by Konica Minolta).

[0164] (Bending test) like Figure 16 As shown, holes 210 with a diameter of 1 mm are made 5 mm from both ends of the 10 mm × 100 mm analytical carrier 1. Nylon threads 220 with a diameter of 0.165 mm are passed through the holes 210, and 10 g E-2 grade weights 230 are respectively installed at both ends of the analytical carrier 1. At the center of the long side of the analytical carrier 1, a porous material is connected to a stainless steel rod 240 with a diameter of 2 mm and a length of 5 cm, thus placing the analytical carrier 1 on the stainless steel rod 240. Figure 17As shown, the stainless steel rod 240 is slowly raised until the weights 230 at both ends of the analytical carrier 1 float and held for 10 seconds, after which the stainless steel rod 240 is returned to its original position. Then, the surface of the porous material 10 is visually observed and evaluated. A condition is "excellent" if there are no residual wrinkles or spalling of the porous material on its surface; "acceptable" if there are residual wrinkles but no spalling; and "unacceptable" if there are residual wrinkles and spalling. Furthermore, if wrinkles of millimeter size or larger are visible to the naked eye, it is evaluated as having residual wrinkles. Additionally, if the porous material 10 separates from the support 20 within the surface of the analytical carrier 1, and the minimum width of the separated portion is 2 mm or more and the maximum length is 5 mm or more, it is evaluated as spalling of the porous material 10.

[0165] (Gold colloid test) The recombinant SARS-CoV-2 nucleoprotein was diluted to concentrations ranging from 0 ng / mL to 100 ng / mL using simulated serum. The resulting liquid was then added dropwise in 100 μL increments to the sample pad 340 of the test strip (refer to...). Figure 18 The analytical carrier was allowed to stand for 20 minutes, and the absorbance of the test and control lines was measured using an immunochromatographic reader (C10066-10, Hamamatsu Photonics). The results are shown in Tables 9 and 10. Figure 19 In addition, the test strips are made as follows.

[0166] <Creating Test Strips> like Figure 18 As shown, an antibody immobilization film 310, an absorption pad 320, a conjugation pad 330, and a sample pad 340 are sequentially attached to a backing plate, and then cut to a width of 5 mm to prepare a test strip 300. The following reagents and materials are prepared when preparing the test strip 300.

[0167] (1) Preparation of reagents and materials Antibody C706: Rabbit monoclonal anti-SARS-CoV-2 nucleoprotein C706, manufactured by HyTest. Antibody C524: Rabbit monoclonal anti-SARS-CoV-2 nucleoprotein C524, manufactured by HyTest. • Anti-rabbit antibody: Goat anti-rabbit IgGh+l affinity purified (A120 201A), manufactured by HyTest. • 5mM-PB (pH 7.0): 3mM disodium hydrogen phosphate, 2mM sodium dihydrogen phosphate dodecahydrate • 1×PBS: Sodium chloride 8 g / L, sodium dihydrogen phosphate dodecahydrate 2.9 g / L, potassium chloride 0.2 g / L, potassium dihydrogen phosphate 0.2 g / L Potassium dihydrogen phosphate: Fujifilm and Wako Pure Chemicals, #169-04245 • Sodium dihydrogen phosphate dodecahydrate: Fujifilm and Wako Pure Chemicals, #193-02845 Sodium chloride: Fujifilm and Washi Pure Chemicals, #191-01665 • Gold colloidal solution: Gold colloidal solution-SC, particle size 40nm, Tanaka precious metals • PEG: Polyethylene glycol 20000, Fuji film, and Koden Chemicals • BSA (albumin, derived from bovine serum, aglobulin-HG): Fujifilm and Koujun Pharmaceutical. • Storage buffer: 1% BSA, 0.1% sodium azide, 0.05% PEG, 20mM Tris-HCl (pH 8.2), 150mM NaCl Sodium azide: Used in Fuji film and Koichi Chemicals. • Simulated serum (composition: 1×PBS, 2% BSA, 0.05% Tween 20) • Antibody solution for the test line: For antibody C524, replace 0.5 mL of Ultracel 30K with 5 mM-PB, and then prepare a solution of 1 mg / mL using 5 mM-PB to achieve a final volume of 100 μL. • Antibody solution for control line: For anti-rabbit antibody, replace 0.5 mL of Ultracel 30K with 5 mM-PB, and then prepare a 0.5 mg / mL solution using 5 mM-PB to achieve a final volume of 100 μL. • Blocking buffer: Gradually add 50mM borax to 50mM boric acid, adjust the pH to 8.5, and then add 2% casein. • Washing buffer: Gradually add 1% hydrochloric acid to 50mM-Tris to adjust the pH to 7.5, then add 0.5% sucrose and 0.05% sodium cholate. • Absorbent pad: Cellulose fiber sample pad 20mm×300mm 100PK (Merck Millipore, CFSP203000) • Backing plate: Pre-cut backing plate (Nippon Engineering, 34042 / 11GL-56338) • Sample pad: Glassfiber Diagnostic Pad

[0168] (2) Fabrication of antibody immobilized thin films The antibody solution for the test line was drawn using a 25 μL syringe (HAMILTON, 702SNR) with a polyethylene capillary attached to the tip. The angle between the membrane surface and the capillary was 45°, and the capillary tip was gently brought into contact with the membrane. The antibody solution was ejected at a rate of 2.5 μL / min, while the test line was scanned at a speed of 24 mm / min. The test line was scanned at a position 7 mm from the bottom of the membrane (18 mm from the top).

[0169] Using a 25 μL syringe (HAMILTON, 702SNR) equipped with a polyethylene capillary tube at the tip, different from the one described above, antibody solution for the control line was drawn. The angle between the membrane surface and the capillary tube was 45°, and the capillary tip was gently brought into contact with the membrane. While ejecting the antibody solution at a rate of 2.5 μL / min, the control line was scanned at a scanning speed of 24 mm / min. The control line was scanned at a position 12 mm from the bottom of the membrane (13 mm from the top).

[0170] The membrane with scanned test and control lines was dried in a 50°C incubator for 1 minute. In a shallow dish filled with blocking buffer to a height of 2 mm, the dried membrane was held upright with the test line below the control line, and allowed to stand for 2 minutes. Next, the membrane was submerged in the blocking buffer and allowed to stand for 3 minutes. Afterward, the membrane was lifted, and excess blocking buffer was shaken off. Next, the membrane was submerged in the washing buffer and allowed to stand for 10 minutes. Afterward, the membrane was lifted, allowing excess washing buffer to seep into the paper, and then dried at 50°C for 10 minutes.

[0171] (3) Making the mat For antibody C706, after replacing 0.5 mL of Ultracel 30K with 5 mM PB, it was prepared to a concentration of 50 μg / mL using ultrapure water to a final volume of 100 μL. To a 2.0 mL tube containing 100 μL of 50 mM potassium dihydrogen phosphate (pH 8.0), 900 μL of gold colloidal solution was added and stirred. While stirring, 100 μL of the prepared 50 μg / mL antibody solution for C706 was added, and the mixture was allowed to stand at room temperature for 10 minutes. 55 μL of 1% PEG was added, and the mixture was gently stirred. 110 μL of 10% BSA (pH 9.0) was added, and the mixture was gently stirred. The mixture was then centrifuged at 8000 G and 4 °C for 15 minutes. The supernatant was removed, leaving approximately 100 μL, and dispersed using an ultrasonic cleaner. Add 2 mL of gold colloidal preservation buffer to the dispersion and centrifuge at 8000 G, 4 °C for 15 minutes. Remove the supernatant after centrifugation, leaving approximately 100 μL of residue, and disperse using an ultrasonic cleaner. Take 1.5 μL of the dispersed sensitized gold colloidal solution and measure its OD520. Based on the measured OD520 value, adjust the OD520 to 6.0 using gold colloidal preservation buffer to prepare the antibody C706 sensitized gold colloidal solution. In a 2 mL tube, mix 420 μL of the antibody C706 sensitized gold colloidal solution, 420 μL of ultrapure water, and 840 μL of spreading buffer. Use a pipette to evenly spread the total volume of the mixture onto a glass fiber diagnostic pad (10 mm × 300 mm), and then place it in a desiccator to dry under reduced pressure for at least 24 hours.

[0172] [Table 1]

[0173] [Table 2]

[0174] [Table 3]

[0175] [Table 4]

[0176] [Table 5]

[0177] [Table 6]

[0178] [Table 7]

[0179] [Table 8]

[0180] [Table 9]

[0181] [Table 10]

[0182] [discuss] As shown in Table 8, the analytical carriers used in Examples 1 to 26 have L Supports with a value of 80 or higher. Therefore, L during drying and during water absorption... With a value above 80, the discriminative power during water absorption is improved. On the other hand, in the analytical carrier of Comparative Example 1, the porous material was not laminated with the support, and the aluminum substrate was not peeled off from the porous material; the L value of the aluminum substrate... The value is below 80. Therefore, although the L during drying... The value is above 80, but the L value during water absorption is... The value is 78. The analytical carriers used in Comparative Examples 2 and 3 possess L... Supports with a value below 80. Therefore, although L during drying... The value is above 80, but the L value during water absorption is... The value is below 80.

[0183] Furthermore, as shown in Table 8, the analytical carriers of Examples 1 to 26, having a support with an adhesive layer, yielded good results in the bending test, with no peeling of the porous material. On the other hand, the analytical carrier of Comparative Example 1 lacked a support with an adhesive layer, and the porous material was integrated with the substrate; therefore, the bending test results were poor, and the porous material peeled off. Based on these results, it can be seen that the analytical carriers of Examples 1 to 26 can suppress the peeling of the porous material from the support even when bending deformation occurs. Furthermore, the analytical carriers of Examples 1 to 20 and Examples 24 to 26 did not experience peeling of the porous material during the bending test, and no residual wrinkles were observed. Based on these results, it can be seen that by keeping the thickness of the outer shell and the thickness of the porous material within a desired range, and by using a support layer formed of a material that is not easily plastically deformed, residual wrinkles can also be suppressed.

[0184] As shown in Tables 9 and 10, in the analytical carriers of Examples 1 to 26, Comparative Examples 2 and 3, the signal strength of the test line and control line was stronger than that of the reference example. Here, as shown in Table 3 and... Figure 15As shown, the ratio of the cumulative pore surface area in the embodiment is 0.1 or more and 10 or less. On the other hand, the ratio of the cumulative pore surface area of ​​the analytical carrier in the reference example is 0.098. Based on these results, it can be seen that in the analytical carrier of this embodiment, there are more pores with a pore size of 0.1 μm or more and less than 1 μm compared with the analytical carrier of the reference example. It is believed that in the analytical carrier of the embodiment, due to the increase in the amount of antibody bound to the porous plasmid and the amount of gold colloid adsorbed on the antibody, the signal of the test line and the control line is enhanced.

[0185] according to Figure 12 The cross-sectional SEM image of the analysis carrier 1 in Example 5 shows that the porous body 10 is supported by a support 20 formed of EVA / LDPE / PET. The adhesive layer 22 formed of EVA is confirmed to be bonded to the outer shell 14 of the porous body 10. A coating layer 17 is confirmed to be provided on the surface of the porous body 10 opposite to the support 20, thereby separating the inner and outer sides of the porous body 10, which contains the skeleton 11 and voids 12.

[0186] according to Figure 13 The surface SEM image of the analysis carrier 1 in Example 5 shows that a coating layer 17 is provided on the surface of the porous body 10. It is confirmed that the hollow particles 13 and the outer shell 14 inside the porous body 10 are covered by the coating layer 17, and the hollow particles 13 and the outer shell 14 are not exposed on the surface side. It is confirmed that the coating layer 17 has connecting holes 18 that extend from the outside to the inside of the porous body 10.

[0187] The entire contents of Japanese Patent Application No. 2024-058092 (application date: March 29, 2024) are incorporated herein by reference.

[0188] The present embodiment has been described above through examples and comparative examples. However, the present embodiment is not limited to these examples and comparative examples, and various modifications can be made within the scope of the present embodiment. Symbol Explanation

[0189] 1. Analytical Carrier 10 porous bodies 11. Skeleton 12 gaps 13 Hollow particles 14. Outer shell 15. Hollow 17. Covering layer 18 connecting holes 20 Supports 100 Sintered Materials 105 aluminum substrate 110 Sintered Body 113 Aluminum metal particles 117 Covering layer 120 Metal Components 130-layer stack.

Claims

1. An analytical carrier comprising a porous body and a support body on one side surface supporting the porous body, wherein, The porous material comprises a framework formed by multiple sets of hollow particles, and multiple voids surrounded by the framework. The hollow particles have an outer shell containing an anodized film comprising aluminum oxide, and cavities surrounded by the outer shell. The skeleton is formed by the continuous shells of the plurality of hollow particles. The porosity of the porous material is greater than 50% by volume and less than 100% by volume. The average pore size of the porous material is greater than 0.1 μm and less than 20 μm. When the analytical carrier, after the porous material has absorbed water, is placed on a white reflectance standard calibrated by the measuring instrument, L is measured using the measuring instrument on the surface of the porous material in the analytical carrier. a b L in the color system The value is above 80.

2. The analytical carrier as described in claim 1, wherein, When the analytical carrier, after drying the porous material, is placed on a white reflectance standard calibrated by the measuring instrument, L is measured using the measuring instrument to measure the surface of the porous material in the analytical carrier. a b L in the color system The value is above 80.

3. The analytical carrier as described in claim 1 or 2, wherein, The support has the porous material on one side surface, i.e., the first surface, and the surface opposite to the first surface, i.e., the second surface, is exposed.

4. The analytical carrier according to any one of claims 1 to 3, wherein, The ratio of metallic aluminum contained in the porous material to the aluminum-containing components constituting the porous material is 0 or more and 0.1 or less by mass.

5. The analytical carrier according to any one of claims 1 to 4, wherein, The support includes an adhesive layer bonded to the porous material and a support layer supporting the adhesive layer. The adhesive layer is bonded to the outer shell, and the adhesive layer is sandwiched between the porous material and the support layer.

6. The analytical carrier as described in claim 5, wherein, The adhesive layer comprises at least one selected from resin, elastomer, starch, and protein.

7. The analytical carrier as described in claim 5 or 6, wherein, The support layer comprises at least one of resin and glass.

8. The analytical carrier according to any one of claims 1 to 7, wherein, The porous body includes a coating layer disposed on the surface opposite to the support, which separates the inner side of the porous body containing the framework and the voids from the outer side of the porous body, and contains an anodized film comprising alumina. The coating layer has connecting pores that connect the inner and outer sides of the porous material. The coating layer is formed continuously with the outer shell of the hollow particles. The cavities of the hollow particles are connected to the outside of the porous body through the connecting holes.

9. The analytical carrier as described in claim 8, wherein, The surface roughness Sa of the porous material is greater than 0.01 μm and less than 1.8 μm.

10. The analytical carrier according to any one of claims 1 to 9, wherein, The thickness of the outer shell is greater than 40nm and less than 1000nm.

11. The analytical carrier according to any one of claims 1 to 10, wherein, The thickness of the porous material is greater than 20 μm and less than 1 mm.

12. The analytical carrier according to any one of claims 1 to 11, wherein, The total surface area of ​​the porous material having pores with a diameter of 0.1 μm or more and less than 100 μm is 0.1 m². 2 / cm 3 Above and 20m 2 / cm 3 the following.

13. The analytical carrier according to any one of claims 1 to 12, wherein, The ratio of the cumulative pore surface area of ​​pores with a pore diameter of 0.1 μm or more and less than 1 μm to the cumulative pore surface area of ​​pores with a pore diameter of 1 μm or more and less than 10 μm is 0.1 or more and less than 10.

14. The analytical carrier according to any one of claims 1 to 13, wherein, The time required to draw water to a height of 4 cm using capillary action is less than 400 seconds.

15. The analytical carrier according to any one of claims 1 to 14, wherein, A 10g weight is installed at each end of the analytical carrier, which is cut to a length of 10mm × 100mm. The analytical carrier is placed on the stainless steel rod in the middle part along the long side of the analytical carrier, with the porous material connected to the stainless steel rod. The carrier is lifted until the weights at both ends of the analytical carrier float up and held for 10 seconds. Then the stainless steel rod is returned to its original position. The porous material does not peel off.

16. An immunochromatographic test strip comprising an analytical carrier according to any one of claims 1 to 15.

17. A method for manufacturing an analytical carrier, comprising a porous material and a support body supporting the porous material on one side surface, wherein... The porous material comprises a framework formed by multiple sets of hollow particles, and multiple voids surrounded by the framework. The hollow particles have an outer shell containing an anodized film comprising aluminum oxide, and cavities surrounded by the outer shell. The skeleton is formed by the continuous shells of the plurality of hollow particles. The manufacturing method includes: The sintering process involves sintering multiple aluminum metal particles on an aluminum substrate to obtain a sintered material comprising the aluminum substrate and a sintered body formed by stacking the aluminum substrate and sintering the aluminum metal particles. An anodizing process is performed on the sintered material to form a shell containing the anodized film on the surface of the aluminum metal particles; and The dissolution process dissolves the aluminum metal particles surrounded by the outer shell. In the manufacturing method, a metal component with the porous body stacked on it is formed on the aluminum substrate by repeatedly performing the anodizing process and the dissolution process. The manufacturing method includes: The lamination process involves bonding the porous material of the metal component to the support, forming a laminate consisting of the aluminum substrate, the porous material, and the support, which are sequentially stacked. The peeling process dissolves the aluminum substrate and peels it off from the laminate. The aluminum metal particles comprise at least one type of aluminum selected from high-purity aluminum, pure aluminum, and aluminum alloys. The aluminum substrate comprises at least one type of aluminum selected from high-purity aluminum, pure aluminum, and aluminum alloys. The average particle size of the plurality of aluminum metal particles is greater than 0.1 μm and less than 20 μm. The filling rate of the sintered body is 10% by volume or more and 60% by volume or less. With the support placed on a white reflectance standard calibrated by the measuring instrument before bonding the porous material, L is measured using the measuring instrument on the surface of the support for bonding the porous material. a b L in the color system The value is above 80.

18. The method for manufacturing an analytical carrier as described in claim 17, wherein, In the anodizing process, a coating layer containing an anodic oxide film comprising aluminum oxide is formed on the surface of the aluminum substrate on which the aluminum metal particles are stacked. In the peeling process, the aluminum substrate is dissolved using an acid solution or an alkaline solution, causing the coating layer formed on the surface of the aluminum substrate opposite to the porous body to separate from the porous body.

19. The method for manufacturing an analytical carrier as described in claim 17 or 18, wherein, The electrolyte used in the anodizing process contains at least one selected from citric acid, boric acid, phosphoric acid, sulfuric acid, oxalic acid, and their salts.

20. The method for manufacturing an analytical carrier according to any one of claims 17 to 19, wherein, The anodizing process and the dissolution process are repeated alternately, with the anodizing process and the dissolution process being repeated more than twice and less than 20 times.

21. The method for manufacturing an analytical carrier according to any one of claims 17 to 20, wherein, The solution used to dissolve the aluminum metal particles in the dissolution process contains at least one acid selected from phosphoric acid, sulfuric acid, nitric acid, and their salts, or at least one base selected from sodium hydroxide and potassium hydroxide.

22. The method for manufacturing an analytical carrier as described in any one of claims 17 to 21, wherein, The stripping solution used in the stripping process contains at least one alkali selected from sodium hydroxide and potassium hydroxide.

23. The method for manufacturing an analytical carrier as described in claim 22, wherein, The stripping solution used in the stripping process contains at least one organic acid selected from citric acid and gluconic acid.

24. The method for manufacturing an analytical carrier as described in any one of claims 17 to 21, wherein, The stripping solution used in the stripping process contains at least one acid selected from phosphoric acid, sulfuric acid, and nitric acid.

25. The method for manufacturing an analytical carrier as described in any one of claims 17 to 24, wherein, The temperature of the stripping solution in the stripping process is above 30°C and below 70°C.

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