Application of 4-cyclopentene-1,3-dione in the control of plant root-knot nematode disease

Abstract

This invention belongs to the technical field of pathogenic microorganism control, and more specifically, relates to the application of 4-cyclopenten-1,3-dione in the control of plant root-knot nematode disease. This invention is the first to discover that 4-cyclopenten-1,3-dione has significant toxic activity against plant root-knot nematodes, and can be used as a novel and effective drug option for the control of plant root-knot nematode disease.

Description

Technical Field

[0001] This invention belongs to the technical field of pathogenic microorganism control, and more specifically, relates to the application of 4-cyclopentene-1,3-dione in the control of plant root-knot nematode disease. Background Technology

[0002] Root-knot nematodes ( Meloidogyne Root-knot nematodes (Spp.) are among the most serious plant parasitic nematodes affecting agricultural production, infecting more than 3,000 plant species and severely impacting crop yield and quality. Currently, over 100 species of root-knot nematodes have been reported worldwide, among which the most economically impactful and widely distributed are: the peanut root-knot nematode (Spp. spp.). M. arenaria ), Northern root-knot nematodes ( M. hapla Javan root-knot nematodes ( M. javanica Southern root-knot nematode ( M. incognita ) and the root-knot nematode ( M. enterolobii Root-knot nematodes, such as the bean weevil, have become one of the most potentially harmful pathogenic nematodes in tropical and subtropical regions worldwide due to their wide host range, high reproductive capacity, high pathogenicity, and ability to overcome existing root-knot nematode resistance genes in many crops. Although root-knot nematode disease seriously damages crops, current control methods have certain limitations.

[0003] Chemical control is a major measure for controlling root-knot nematode disease in agricultural production. Due to its advantages such as rapid effectiveness and cost-effectiveness, it is considered a relatively effective means of nematode control. Currently, commonly used chemical control agents mainly include nematicides such as abamectin and thiazophos, as well as soil fumigants with dazomet as the main component. However, due to the relatively limited types of available pesticides, long-term or unreasonable use can easily lead to nematode resistance, and may also cause problems such as damage to the soil microecology and increased ecological risks. Patent application CN121753794A discloses the application of 2-hydroxy-3-methyl-2-cyclopenten-1-one in plant-parasitic nematodes; however, its activity and inhibitory effect on plant root-knot nematodes are poor. At a concentration of 50 mg / L, the corrected mortality rate for larvae at 24 h was 9.46%, LC... 50 The concentration is 290 mg / L, requiring a high dosage and concentration to achieve a good effect in inhibiting plant root-knot nematodes.

[0004] Therefore, the discovery of novel and highly effective compounds with higher inhibitory activity against plant root-knot nematodes, which can effectively prevent and control root-knot nematode disease, has become a key issue that urgently needs to be addressed in the current agricultural field. Summary of the Invention

[0005] In view of the aforementioned problems in the prior art, the primary objective of this invention is to provide the application of 4-cyclopentene-1,3-dione in inhibiting plant root-knot nematodes or in the prevention and control of plant root-knot nematode diseases.

[0006] A second objective of this invention is to provide the application of 4-cyclopentene-1,3-dione in the preparation of products for the prevention and control of plant root-knot nematode disease.

[0007] A third objective of this invention is to provide an agricultural composition for the suppression of plant root-knot nematodes or for the prevention and control of plant root-knot nematode disease.

[0008] The fourth objective of this invention is to provide a method for preventing and controlling plant root-knot nematode disease.

[0009] To achieve the above objectives, the present invention is implemented through the following technical solution: This invention seeks protection for the use of 4-cyclopentene-1,3-dione in inhibiting plant root-knot nematodes or in the prevention and control of plant root-knot nematode disease.

[0010] This invention is the first to discover that 4-cyclopentene-1,3-dione exhibits significant toxic activity against plant root-knot nematodes, making it a novel and effective option for the control of plant root-knot nematode diseases. The 4-cyclopentene-1,3-dione described in this invention can be one of the main components of tobacco processing waste. Applying plant-derived 4-cyclopentene-1,3-dione to the control of root-knot nematodes not only enriches the variety of existing nematode control agents and delays the development of resistance, but also reduces adverse impacts on soil microecology and the overall ecological environment, aligning with the overall requirements of green and high-quality agricultural development in my country. Furthermore, 4-cyclopentene-1,3-dione also possesses fumigation activity, enabling the control of plant root-knot nematodes through more diverse and comprehensive mechanisms of action.

[0011] Specifically, the 4-cyclopenten-1,3-dione has the CAS number 930-60-9, a relative molecular mass of 96.08, and a structural formula as shown in formula (I):

[0012] (I).

[0013] Specifically, the 4-cyclopenten-1,3-dione can be synthesized by referring to existing known literature methods, or it can be obtained directly through commercial means, such as purchasing it from Anhui Zesheng Technology Co., Ltd.

[0014] Preferably, the purity of the 4-cyclopentene-1,3-dione is ≥90%; more preferably, the purity of the 4-cyclopentene-1,3-dione can be ≥91%, ≥92%, ≥93%, ≥94%, ≥95%, ≥96%, ≥97%, ≥98%, ≥99%, ≥99.5%, ≥99.8%, ≥99.9%, etc., and the present invention is not limited thereto.

[0015] Preferably, the plant root-knot nematode includes *Eriocheir sinensis* (also known as the weevils' root-knot nematode). Meloidogyne enterolobii Southern root-knot nematode ( Meloidogyne incognita Javan root-knot nematodes ( Meloidogyne javanica ), peanut root-knot nematode ( Meloidogyne arenaria ) and northern root-knot nematodes ( Meloidogyne hapla At least one of the following. More preferably, the plant root-knot nematode is *Eriocheir weevil*. The inventors have discovered through research that 4-cyclopentene-1,3-dione has a more significant effect on the prevention and control of *Eriocheir weevil* disease.

[0016] Preferably, the plant includes at least one of the following: Solanaceae, Cucurbitaceae, Brassicaceae, Fabaceae, Poaceae, Apiaceae, Amaranthaceae / Chenamiaceae, Convolvulaceae, Malvaceae, Rosaceae, Moraceae, Vitaceae, Myrtaceae, Euphorbiaceae, Lamiaceae, Rubiaceae, Caricaceae, Musaceae, Dioscoreaceae, Zingiberaceae, Theaceae, Cactaceae, Malvaceae, Cannabisaceae, and Basellaceae.

[0017] More preferably, the Solanaceae plants include, but are not limited to, tomatoes, eggplants, peppers, tobacco, and potatoes. The Cucurbitaceae plants include, but are not limited to, cucumbers, loofahs, bitter melons, cantaloupes, pumpkins, watermelons, and winter melons. The Cruciferae plants include, but are not limited to, cabbage, rapeseed, mustard greens, kale, broccoli, and cauliflower. The Leguminosae plants include, but are not limited to, soybeans, cowpeas, kidney beans, lentils, alfalfa, peanuts, and peas. The Gramineae plants include, but are not limited to, corn, wheat, rice, sugarcane, sweet corn, Sudan grass, tall fescue, reed fescue, sweet sorghum, and golden millet. The Apiaceae plants include, but are not limited to, carrots, celery, and parsley. The Chenopodiaceae plants include, but are not limited to, spinach and beets. The Convolvulaceae plants include, but are not limited to, sweet potatoes. The Malvaceae plants include, but are not limited to, cotton, jute, and kenaf. The Rosaceae plants include, but are not limited to, peaches, roses, and apples. The Vitaceae plants include, but are not limited to, grapes.

[0018] Preferably, 4-cyclopentene-1,3-dione is used throughout the entire course of plant root-knot nematode disease.

[0019] Preferably, the method of application is selected from at least one of (a) and (b); (a) Contact killing of plant root-knot nematodes by contacting a solution containing 4-cyclopentene-1,3-dione with the plant root-knot nematode, wherein the concentration of 4-cyclopentene-1,3-dione in the solution is ≥12.5 mg / L; (b) Fumigation poisoning using the gas produced by the volatilization of 4-cyclopenten-1,3-dione, wherein the gaseous mass concentration of 4-cyclopenten-1,3-dione in the space is ≥0.4353 mg·L. -1 air.

[0020] More preferably, the method of application is selected from at least one of (a) and (b); (a) Contact killing of plant root-knot nematodes by contacting a solution containing 4-cyclopentene-1,3-dione with the plant root-knot nematode, wherein the concentration of 4-cyclopentene-1,3-dione in the solution is ≥25 mg / L; (b) Fumigation poisoning using the gaseous product of 4-cyclopenten-1,3-dione volatilization, wherein the gaseous mass concentration of 4-cyclopenten-1,3-dione in the space is ≥0.5079 mg·L. -1 air.

[0021] Preferably, in the above method (a), the concentration of 4-cyclopenten-1,3-dione in the solution can be 12.5-50 mg / L, 15-50 mg / L, 20-50 mg / L, 25-50 mg / L, 20-30 mg / L, or any range formed by the above values, and the present invention is not limited thereto.

[0022] Preferably, in method (b) above, the concentration of 4-cyclopenten-1,3-dione in the solution can be from 0.4353 to 0.9000 mg·L⁻¹. -1 air, 0.4353 to 0.6000 mg·L -1 air, 0.4353 to 0.6046 mg·L -1 air, 0.4353 to 0.5079 mg·L -1 The present invention is not limited to air, or any range formed by the above values.

[0023] Preferably, in methods (a) and (b) above, the solvent of the solution can be selected from good solvents that have good solubility for 4-cyclopenten-1,3-dione; more specifically, good solvents include, but are not limited to, water, alcohol solvents (such as methanol, ethanol, isopropanol, etc.), ketone solvents (such as acetone, etc.), ester solvents (such as ethyl acetate, etc.), halogenated hydrocarbon solvents (such as dichloromethane, trichloromethane, etc.), ether solvents (such as diethyl ether, tetrahydrofuran, 1,4-dioxane, etc.), and polar aprotic solvents (such as dimethyl sulfoxide, N,N-dimethylformamide, acetonitrile, etc.).

[0024] Preferably, the plant root-knot nematode diseases include cucumber root-knot nematode disease, tomato root-knot nematode disease, eggplant root-knot nematode disease, pepper root-knot nematode disease, potato root-knot nematode disease, lettuce root-knot nematode disease, carrot root-knot nematode disease, Chinese cabbage root-knot nematode disease, spinach root-knot nematode disease, common bean root-knot nematode disease, cowpea root-knot nematode disease, hyacinth bean root-knot nematode disease, guava root-knot nematode disease, grape root-knot nematode disease, peach root-knot nematode disease, fig root-knot nematode disease, kiwifruit root-knot nematode disease, peanut root-knot nematode disease, soybean root-knot nematode disease, and cotton root-knot nematode disease. The following diseases are considered to be root-knot nematode diseases: tobacco root-knot nematode disease, sweet potato root-knot nematode disease, beet root-knot nematode disease, kenaf root-knot nematode disease, maize root-knot nematode disease, wheat root-knot nematode disease, rice root-knot nematode disease, ginseng root-knot nematode disease, Panax notoginseng root-knot nematode disease, American ginseng root-knot nematode disease, Scutellaria baicalensis root-knot nematode disease, Dioscorea opposita root-knot nematode disease, Salvia miltiorrhiza root-knot nematode disease, rose root-knot nematode disease, Lantana camara root-knot nematode disease, Cinnamomum camphora root-knot nematode disease, Perilla frutescens root-knot nematode disease, Thalictrum lucidum root-knot nematode disease, Paeonia suffruticosa root-knot nematode disease, Cyclamen root-knot nematode disease, and London plane tree root-knot nematode disease.

[0025] Preferably, the formulation of the agricultural composition includes at least one of the following: aqueous solution, emulsion, wettable powder, soluble powder, soluble granules, and suspension concentrate.

[0026] Furthermore, the present invention claims protection for the use of an agricultural composition in inhibiting plant root-knot nematodes or in controlling plant root-knot nematode disease, said agricultural composition having 4-cyclopentene-1,3-dione as the main active ingredient or one of the active ingredients.

[0027] Furthermore, the present invention claims protection for a method for controlling plant root-knot nematode disease, which involves contact killing of plant root-knot nematodes by exposing a solution containing 4-cyclopentene-1,3-dione to the plant.

[0028] Preferably, the contact killing method includes at least one of soaking, seed soaking, root dipping, root irrigation, fertigation, drip irrigation, application of pesticide with water, spraying, watering, application of solution in holes, application of solution in trenches, application of water at planting, seedbed treatment, substrate treatment, soil leaching treatment, and supplemental application in the root zone during the growing season.

[0029] Furthermore, this invention claims protection for a method for preventing and controlling plant root-knot nematode disease, which uses fumigation to kill the nematode by means of gas generated by the volatilization of 4-cyclopentene-1,3-dione.

[0030] Preferably, the method of exposing plant root-knot nematodes to a gas containing 4-cyclopentene-1,3-dione includes at least one of fumigation, slow-release volatilization treatment, and volatilization carrier release treatment.

[0031] More preferably, fumigation includes, but is not limited to, soil fumigation, substrate fumigation, sealed container fumigation, facility space fumigation, film fumigation, fumigation after trench application and covering with soil, fumigation after hole application and covering with soil, and fumigation after mixing with soil and covering with film.

[0032] Compared with the prior art, the present invention has the following beneficial effects: This invention is the first to discover that 4-cyclopentene-1,3-dione has significant toxic activity against plant root-knot nematodes and can be used to control plant root-knot nematode disease, serving as a novel and effective option for the prevention and control of plant root-knot nematode disease. Attached Figure Description

[0033] Figure 1 A bar chart showing the indoor toxicity determination of 4-cyclopentene-1,3-dione against *Auricularia auricula-judae* nematode in Example 1 after 24 h of treatment.

[0034] Figure 2 A bar chart showing the indoor toxicity determination of 4-cyclopentene-1,3-dione against *Auricularia auricula-judae* nematode in Example 1 after 48 h of treatment.

[0035] Figure 3 The toxicity regression curve for the indoor toxicity determination of 4-cyclopentene-1,3-dione against *Auricularia auricula-judae* nematode in Example 1 after 24 h of treatment.

[0036] Figure 4 The toxicity regression curve for the indoor toxicity determination of 4-cyclopentene-1,3-dione against *Auricularia auricula-judae* nematode in Example 1 after 48 h of treatment.

[0037] Figure 5 This is a bar chart showing the indoor fumigation activity of 4-cyclopentene-1,3-dione against *Auricularia auricula-judae* nematode after 24 hours of treatment in Example 2.

[0038] Figure 6 The image shows the toxicity regression curve of 4-cyclopentene-1,3-dione on the indoor fumigation activity of *Auricularia auricula-judae* nematode after 24 hours of treatment in Example 2. Detailed Implementation

[0039] The present invention will be further described below with reference to the specification and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0040] Example 14: Indoor toxicity determination of cyclopentene-1,3-dione against *Auricularia auricula-judae* root-knot nematode. 1. Experimental Methods Preparation of the test solution: 10 mg of 4-cyclopenten-1,3-dione (CAS No. 930-60-9) was dissolved in 1 mL of dimethyl sulfoxide (DMSO) to obtain a stock solution with a concentration of 10 mg / mL. This stock solution was then diluted with 0.1% Tween-80 aqueous solution to final concentrations of 50 mg / L, 25 mg / L, 20 mg / L, 16 mg / L, 12.5 mg / L, and 6.25 mg / L. Since the final experimental procedure involved adding 100 μL of the stock solution and 100 μL of nematode suspension to a 96-well plate, the effective concentrations of 4-cyclopenten-1,3-dione in the toxicity test system were 25 mg / L, 12.5 mg / L, 10 mg / L, 8 mg / L, 6.25 mg / L, and 3.125 mg / L, respectively. The test solution for the blank control group was a 0.1% Tween-80 aqueous solution.

[0041] Pepper roots infected with *Heliotropium indicum* were washed with clean water to remove surface dirt and sand. They were then disinfected by soaking in a 0.01% NaClO aqueous solution for 5 minutes. Oocysts of the root-knot nematodes were collected using a dissecting needle and incubated in sterile water for 24 hours. Nematodes were then enriched using the Bellman funnel method for 24 hours. The collected nematodes were diluted with sterile water to prepare a nematode suspension of 100 nematodes / 100 μL. 100 μL of different concentrations of the test solution and nematode suspension were injected into 96-well plates, sealed, and incubated at 25°C in the dark. The mortality rate of the nematodes was checked after 24 and 48 hours of treatment. The nematode rigidity and lack of response to mechanical stimulation were used as the criteria for death. Each treatment was repeated three times. The nematode mortality rate was calculated as follows: Mortality rate (%) = (Number of dead nematodes / Number of tested nematodes) × 100%.

[0042] 2. Test Results like Figure 1 and Figure 2 As shown, the concentration of 4-cyclopentene-1,3-dione solution showed a linear relationship with the mortality rate of *Anoplophora glomerata* root-knot nematodes; the higher the solution concentration, the higher the nematode mortality rate. After 24 h of treatment, the median lethal concentration (LC50) of 4-cyclopentene-1,3-dione for *Anoplophora glomerata* root-knot nematodes was... 50 The nematode mortality rate began to appear in the treatment group when the drug concentration was 10.91 mg / L and 6.25 mg / L, with an average mortality rate of 0.88%. The mortality rate accelerated when the drug concentration was 12.5 mg / L, with an average mortality rate of 72.32%. The mortality rate was highest when the drug concentration was 25 mg / L, with an average mortality rate of 98.38%.

[0043] The median lethal concentration (LC50) of 4-cyclopentene-1,3-dione against *Auricularia auricula-judae* root-knot nematode after 48 h of treatment. 50The concentration of the drug solution was 9.886 mg / L. Nematodes began to die in the treatment group when the drug solution concentration was 3.125 mg / L, with an average mortality rate of 1.53%. The mortality rate of nematodes accelerated when the drug solution concentration was 8 mg / L, with an average mortality rate of 37.65%. The mortality rate of nematodes was the highest when the drug solution concentration was 25 mg / L, with an average mortality rate of 98.76%.

[0044] like Figure 3 and Figure 4 As shown, toxicity regression curves for the 24-h and 48-h indoor toxicity assays of 4-cyclopentene-1,3-dione against *Heliotropium indicum* were plotted based on the average mortality rate of *Heliotropium indicum* under different concentration treatments. The toxicity regression equation at 24 hours after treatment was y = -1.47 + 6.08x, R0. 2 =0.926, LC 50 =10.91 mg / L, 95% confidence limit range is 10.25~11.684 mg / L. The toxicity regression equation 48 hours after treatment is y=-0.73+5.85x, R0 2 =0.941, LC 50 =9.886 mg / L, with a 95% confidence limit range of 9.448–10.34 mg / L. This indicates that the nematode population is highly sensitive to changes in pesticide concentration; even a small increase in concentration can significantly enhance the killing effect.

[0045] Example 24: Determination of the fumigation activity of cyclopentene-1,3-dione against *Auricularia auricula-judae* root-knot nematode. 1. Experimental Methods The experiment used a two-compartment culture dish method. 10.0 mg of 4-cyclopenten-1,3-dione was weighed and added to 100 µL of sterile water. After shaking and mixing, serial dilutions were performed to obtain different solutions containing 0.042 mg, 0.036 mg, 0.030 mg, 0.024 mg, and 0.012 mg of 4-cyclopenten-1,3-dione per 100 µL. Sterile water was used as a blank control. Two-compartment culture dishes with a diameter of 90 mm and a height of 13 mm were used. One side of the dish contained 100 µL of the test solution, and the other side contained 100 µL of nematode suspension (prepared as in Example 1) using a pipette. The dishes were sealed and incubated at 25°C in the dark for 24 h. The results were checked afterward, and the gaseous concentration of 4-cyclopenten-1,3-dione in the air within the compartments was measured. Sterile water was used as a blank control, and each treatment was repeated in triplicate. The number of dead root-knot nematodes in each well was examined using a stereomicroscope. The nematode was deemed dead when it was rigid and unresponsive to mechanical stimulation. The mortality rate was calculated using the same formula as in Example 1.

[0046] 2. Test Results like Figure 5 As shown, the gaseous mass concentration of 4-cyclopentene-1,3-dione exhibits a linear relationship with the mortality rate of *Euonymus alatus* root-knot nematodes; the higher the gaseous mass concentration, the higher the nematode mortality rate. The median lethal concentration (LC50) of *Euonymus alatus* root-knot nematodes after 24 h of fumigation with 4-cyclopentene-1,3-dione is also shown. 50 The value was 0.3265 mg·L. -1 air, gas phase mass concentration is 0.1451 mg·L⁻¹ - 1 Nematode mortality began in the treatment group when air (corresponding to 0.012 mg of 4-cyclopenten-1,3-dione) was applied, with an average mortality rate of 7.26% at a gas phase concentration of 0.4353 mg·L⁻¹. -1 When air was used (corresponding to 0.036 mg of 4-cyclopenten-1,3-dione), the mortality rate of nematodes accelerated, with an average mortality rate of 80.39% at a gas phase concentration of 0.5079 mg·L⁻¹. - 1 The mortality rate of nematodes was highest when air was used (corresponding to a dosage of 0.042 mg of 4-cyclopentene-1,3-dione), with an average mortality rate of 90.03%.

[0047] like Figure 6 As shown, toxicity regression curves of 4-cyclopentene-1,3-dione fumigation treatment with *Heliotropium indicum* for 24 h were plotted based on the average mortality rate of *Heliotropium indicum* under various gas phase mass concentrations. The toxicity regression equation 24 h after treatment is y = 7.44 + 4.92x, R0. 2 =0.912, LC 50 =0.3265 mg·L -1 air, the 95% confidence limit range is 0.3023 ~ 0.3507 mg·L -1 The presence of air indicates that the nematode population is highly sensitive to changes in the gaseous mass concentration of 4-cyclopentene-1,3-dione; a slight increase in the gaseous mass concentration can significantly enhance the toxic effect.

[0048] The foregoing examples are merely illustrative, used to explain some features of the method described in this invention. The appended claims are intended to claim the broadest possible scope, and the embodiments presented herein are demonstrated by the applicant's actual experimental results. Therefore, the applicant intends that the appended claims are not limited by the selection of examples illustrating the features of the invention. Some numerical ranges used in the claims also include sub-ranges within them, and variations within these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

Application of 1,4-cyclopentene-1,3-dione in inhibiting plant root-knot nematodes or in the prevention and control of plant root-knot nematode disease.

2. The application according to claim 1, characterized in that, The plant root-knot nematodes include the weevils root-knot nematode ( ). Meloidogyne enterolobii Southern root-knot nematode ( Meloidogyne incognita Javan root-knot nematodes ( Meloidogyne javanica ), peanut root-knot nematode ( Meloidogyne arenaria ) and northern root-knot nematodes ( Meloidogyne hapla At least one of the following.

3. The application according to claim 1, characterized in that, The plants include at least one of the following: Solanaceae, Cucurbitaceae, Brassicaceae, Fabaceae, Poaceae, Apiaceae, Amaranthaceae / Chenuraceae, Convolvulaceae, Malvaceae, Rosaceae, Moraceae, Vitaceae, Myrtaceae, Euphorbiaceae, Lamiaceae, Rubiaceae, Caricaceae, Musaceae, Dioscoreaceae, Zingiberaceae, Theaceae, Cactaceae, Malvaceae, Cannabisaceae, and Basellaceae.

4. The application according to claim 1, characterized in that, 4-Cyclopentene-1,3-dione was used throughout the entire course of root-knot nematode disease in plants.

5. The application according to claim 1, characterized in that, The method of application is selected from at least one of (a) and (b); (a) Contact killing of plant root-knot nematodes by contacting a solution containing 4-cyclopentene-1,3-dione with the plant root-knot nematode, wherein the concentration of 4-cyclopentene-1,3-dione in the solution is ≥25 mg / L; (b) Fumigation poisoning using the gas produced by the volatilization of 4-cyclopenten-1,3-dione, wherein the gaseous mass concentration of 4-cyclopenten-1,3-dione in the space is ≥0.4353 mg·L. -1 air.

6. The application according to claim 1, characterized in that, The plant root-knot nematode diseases mentioned include those of cucumber, tomato, eggplant, pepper, potato, lettuce, carrot, cabbage, spinach, bean, cowpea, hyacinth bean, guava, grape, peach, fig, kiwifruit, peanut, soybean, cotton, and tobacco. Root-knot nematode disease, sweet potato root-knot nematode disease, beet root-knot nematode disease, kenaf root-knot nematode disease, maize root-knot nematode disease, wheat root-knot nematode disease, rice root-knot nematode disease, ginseng root-knot nematode disease, Panax notoginseng root-knot nematode disease, American ginseng root-knot nematode disease, Scutellaria baicalensis root-knot nematode disease, Dioscorea opposita root-knot nematode disease, Salvia miltiorrhiza root-knot nematode disease, rose root-knot nematode disease, Lantana camara root-knot nematode disease, red-backed cinnamon root-knot nematode disease, Perilla frutescens root-knot nematode disease, Thalictrum lucidum root-knot nematode disease, peony root-knot nematode disease, cyclamen root-knot nematode disease, and London plane tree root-knot nematode disease.

7. The application of an agricultural composition in inhibiting plant root-knot nematodes or in controlling plant root-knot nematode diseases, characterized in that, The agricultural composition uses 4-cyclopentene-1,3-dione as the main active ingredient or one of the active ingredients.

8. The application according to claim 7, characterized in that, The formulation of the agricultural composition includes at least one of the following: aqueous solution, emulsion, wettable powder, soluble powder, soluble granules, and suspension.

9. A method for controlling plant root-knot nematode disease, characterized in that, Contact killing of plant root-knot nematodes involves exposing a solution containing 4-cyclopentene-1,3-dione to the plant.

10. A method for controlling plant root-knot nematode disease, characterized in that, The method of fumigation and poisoning is achieved by using the gas generated from the volatilization of 4-cyclopentene-1,3-dione.

Images