Paris yellow ginseng antioxidant composition and application thereof

Abstract

The present application belongs to the field of cosmetics, and relates to an antioxidant composition and application thereof. The antioxidant composition is composed of Paris extract and Astragalus extract. The antioxidant composition provided by the present application has synergistic antioxidant effect, and can be applied to the preparation of skin care products with soothing, repairing and other effects.

Description

[0001] This application is a divisional application of Chinese Patent Application No. 202610531712.9, filed on April 21, 2026, with a priority date of December 26, 2025, entitled "Antioxidant Composition and Its Application". This application claims priority to Chinese Patent Application No. 202511995560.X, filed on December 26, 2025. Technical Field

[0002] This invention belongs to the field of cosmetics and relates to an antioxidant composition of Paris polyphylla and Astragalus membranaceus and its application. Background Technology

[0003] Skin damage and anti-oxidation are increasingly becoming important directions in cosmetic research and development. With the widespread application of traditional Chinese medicine extracts in the cosmetic field, their unique efficacy is receiving increasing attention. Traditional Chinese medicine compositions have significant effects on skin soothing and repair, and possess good safety and biocompatibility. However, current cosmetics on the market still suffer from problems such as relatively limited product functions, poor user experience, and poor skin absorption. Therefore, developing a safe, effective, multifunctional cosmetic composition with synergistic effects has high application value. Summary of the Invention

[0004] In some embodiments, the present invention provides an antioxidant composition comprising any two of Paris polyphylla extract, Astragalus membranaceus extract, and Poria cocos extract; wherein the Paris polyphylla extract is present in a weight fraction of 1.25 to 5 parts, the Astragalus membranaceus extract in a weight fraction of 7.5 to 30 parts, and the Poria cocos extract in a weight fraction of 0.5 to 2 parts.

[0005] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 7.5 to 30 parts of Astragalus membranaceus extract and 0.5 to 2 parts of Poria cocos extract.

[0006] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 7.5 to 25 parts of Astragalus membranaceus extract and 0.75 to 2 parts of Poria cocos extract.

[0007] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 7.5 to 20 parts of Astragalus membranaceus extract and 0.9 to 1.9 parts of Poria cocos extract.

[0008] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 7.5 to 18 parts of Astragalus membranaceus extract and 1 to 1.8 parts of Poria cocos extract.

[0009] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 8-18 parts of Astragalus membranaceus extract and 1-1.7 parts of Poria cocos extract.

[0010] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 9-17 parts of Astragalus membranaceus extract and 1-1.6 parts of Poria cocos extract.

[0011] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 10-16 parts of Astragalus membranaceus extract and 1-1.5 parts of Poria cocos extract.

[0012] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 15 parts Astragalus extract and 1 part Poria cocos extract.

[0013] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.25 to 5 parts of Paris polyphylla extract and 0.5 to 2 parts of Poria cocos extract.

[0014] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.25 to 4.5 parts of Paris polyphylla extract and 0.6 to 1.9 parts of Poria cocos extract.

[0015] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.25 to 4 parts of Paris polyphylla extract and 0.7 to 1.8 parts of Poria cocos extract.

[0016] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.75 to 4.5 parts of Paris polyphylla extract and 0.7 to 1.8 parts of Poria cocos extract.

[0017] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.25 to 3.5 parts of Paris polyphylla extract and 0.8 to 1.7 parts of Poria cocos extract.

[0018] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2 to 4.25 parts of Paris polyphylla extract and 1 to 1.7 parts of Poria cocos extract.

[0019] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.25 to 3 parts of Paris polyphylla extract and 0.9 to 1.6 parts of Poria cocos extract.

[0020] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2-4 parts of Paris polyphylla extract and 1-1.6 parts of Poria cocos extract.

[0021] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.25 to 2.5 parts of Paris polyphylla extract and 0.9 to 1.4 parts of Poria cocos extract.

[0022] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2.25 to 3.5 parts of Paris polyphylla extract and 1 to 1.5 parts of Poria cocos extract.

[0023] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2.5 parts of Paris polyphylla extract and 1 part of Poria cocos extract.

[0024] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.25 to 5 parts of Paris polyphylla extract and 7.5 to 30 parts of Astragalus membranaceus extract.

[0025] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.5 to 4.75 parts of Paris polyphylla extract and 7.5 to 20 parts of Astragalus membranaceus extract.

[0026] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 1.75 to 4.5 parts of Paris polyphylla extract and 7.5 to 18 parts of Astragalus membranaceus extract.

[0027] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2-4.25 parts of Paris polyphylla extract and 8-18 parts of Astragalus membranaceus extract.

[0028] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2 to 4.25 parts of Paris polyphylla extract and 7.5 to 16 parts of Astragalus membranaceus extract.

[0029] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2-4 parts of Paris polyphylla extract and 9-17 parts of Astragalus membranaceus extract.

[0030] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2-4 parts of Paris polyphylla extract and 7.5-14 parts of Astragalus membranaceus extract.

[0031] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2.25 to 3.5 parts of Paris polyphylla extract and 10 to 16 parts of Astragalus membranaceus extract.

[0032] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2.25 to 3.5 parts of Paris polyphylla extract and 7.5 to 12 parts of Astragalus membranaceus extract.

[0033] In some embodiments, the antioxidant composition comprises the following components in parts by weight: 2.5 parts of Paris polyphylla extract and 15 parts of Astragalus membranaceus extract.

[0034] In some embodiments, the Paris polyphylla extract is an alcoholic extract of Paris polyphylla.

[0035] In some embodiments, the Paris polyphylla extract is an ethanol extract of Paris polyphylla.

[0036] In some implementations, the extraction method of the Paris polyphylla extract includes the following steps: (1) The crude powder of Paris polyphylla was soaked in an ethanol aqueous solution and then refluxed to extract the extract; (2) Filter the extract obtained in (1) and concentrate it to obtain a concentrated solution; (3) Load the concentrated solution obtained in (2) onto the adsorption resin, perform gradient elution, and collect the eluent; (4) Concentrate and dry the eluent obtained from (3) to obtain Paris polyphylla extract.

[0037] In some implementations, the ethanol-water solution in step (1) is 50%-90% v / v.

[0038] In some implementations, the ethanol-water solution in step (1) is 60%-85% v / v.

[0039] In some implementations, the ethanol-water solution in step (1) is 70%-80% v / v.

[0040] In some implementations, the soaking time is 10-120 min.

[0041] In some implementations, the soaking time is 15-90 minutes.

[0042] In some implementations, the soaking time is 20-60 minutes.

[0043] In some implementations, the reflux extraction is performed 1-10 times.

[0044] In some implementations, the reflux extraction is performed 2-8 times.

[0045] In some implementations, the reflux extraction is performed 3-6 times.

[0046] In some implementations, the reflux extraction time is 1-10 h / time.

[0047] In some implementations, the reflux extraction time is 2-8 hours per cycle.

[0048] In some implementations, the reflux extraction time is 2-5 hours per cycle.

[0049] In some implementations, the filter used for filtration is a 100-500 mesh filter.

[0050] In some implementations, the filter used for filtration is a 150-300 mesh filter.

[0051] In some implementations, the concentration in step (2) is selected from atmospheric pressure concentration or vacuum concentration.

[0052] In some implementations, the concentration described in step (2) is selected from vacuum concentration.

[0053] In some embodiments, the adsorption resin is selected from macroporous adsorption resins.

[0054] In some implementations, the sample loading flow rate is 0.1-6 BV / h.

[0055] In some implementations, the sample loading flow rate is 0.5-5 BV / h.

[0056] In some implementations, the sample loading flow rate is 0.8-4 BV / h.

[0057] In some implementations, the elution flow rate is 0.5-6 BV / h.

[0058] In some implementations, the elution flow rate is 1-5 BV / h.

[0059] In some implementations, the elution flow rate is 1.5-4 BV / h.

[0060] In some embodiments, the gradient elution is performed by sequentially using pure water, 10%-35% v / v ethanol aqueous solution, 36%-55% v / v ethanol aqueous solution, 56%-70% v / v ethanol aqueous solution, 71%-85% v / v ethanol aqueous solution, and 86%-99% v / v ethanol aqueous solution as eluents.

[0061] In some embodiments, the gradient elution is performed by sequentially using pure water, 15%-35% v / v ethanol aqueous solution, 40%-55% v / v ethanol aqueous solution, 60%-70% v / v ethanol aqueous solution, 75%-85% v / v ethanol aqueous solution, and 90%-99% v / v ethanol aqueous solution as eluents.

[0062] In some embodiments, the gradient elution is performed by sequentially using pure water, 30% v / v ethanol aqueous solution, 50% v / v ethanol aqueous solution, 65% v / v ethanol aqueous solution, 80% v / v ethanol aqueous solution and 95% v / v ethanol aqueous solution as eluents.

[0063] In some implementations, the volume of different eluents eluted in the gradient elution is 1-5 BV.

[0064] In some implementations, the volume of different eluents eluted in the gradient elution is 1-4 BV.

[0065] In some implementations, the volume of different eluents eluted in the gradient elution is 2-3 BV.

[0066] In some implementations, the concentration in step (4) is selected from atmospheric pressure concentration or vacuum concentration.

[0067] In some implementations, the concentration described in step (4) is selected from vacuum concentration.

[0068] In some implementations, the drying is vacuum drying.

[0069] In some implementation schemes, the Paris polyphylla is selected from Paris polyphylla yunnanensis, Paris polyphylla sphericalis, Paris polyphylla var. chinensis, Paris polyphylla var. septemlobus, Paris polyphylla var. daliensis, Paris polyphylla var. polyphylla, Paris polyphylla var. septemlobus ... or Paris polyphylla var. septemlobus.

[0070] In some implementations, the Paris polyphylla is selected from Paris polyphylla var. spp.

[0071] In some embodiments, the astragalus extract is an ammonia extract of astragalus.

[0072] In some embodiments, the extraction method of the Astragalus extract includes the following steps: (1) soaking the crude Astragalus powder in ammonia water and then refluxing to extract the extract; (2) Concentrate the extract obtained in (1) to obtain an extract; (3) Dry the extract obtained in (2) to obtain Astragalus extract.

[0073] In some implementations, the ammonia water in step (1) is 1~20 wt%.

[0074] In some implementations, the ammonia water in step (1) is 2~15 wt%.

[0075] In some implementations, the ammonia water in step (1) is 5~10 wt%.

[0076] In some implementations, the soaking time is 1-40 hours.

[0077] In some implementations, the soaking time is 5-35 hours.

[0078] In some implementations, the soaking time is 10-30 hours.

[0079] In some implementations, the reflux extraction is performed 1-10 times.

[0080] In some implementations, the reflux extraction is performed 2-8 times.

[0081] In some implementations, the reflux extraction is performed 3-6 times.

[0082] In some implementations, the reflux extraction time is 1-10 h / time.

[0083] In some implementations, the reflux extraction time is 2-8 hours per cycle.

[0084] In some implementations, the reflux extraction time is 2-5 hours per cycle.

[0085] In some implementations, the mass ratio of the extract to the paste in step (2) is 1:(0.5-2).

[0086] In some implementations, the mass ratio of the extract to the paste in step (2) is 1:(0.8-1.25).

[0087] In some implementations, the mass ratio of the extract to the paste in step (2) is 1:(0.9-1.1).

[0088] In some implementations, the Astragalus membranaceus is selected from Astragalus mongholicus or Astragalus membranaceus.

[0089] In some embodiments, the Poria cocos extract is an aqueous extract of Poria cocos.

[0090] In some implementation schemes, the extraction method of the Poria cocos extract includes the following steps: (1) soaking the crude Poria cocos powder in water and then refluxing it to extract the extract; (2) Filter the extract obtained in (1) and concentrate it to obtain a concentrated solution; (3) Load the concentrated solution obtained in (2) onto the adsorption resin, perform gradient elution, and collect the eluent; (4) Concentrate and dry the eluent obtained in step (3) to obtain Poria cocos extract.

[0091] In some implementations, the temperature of the water in step (1) is 50-100°C.

[0092] In some implementations, the temperature of the water in step (1) is 60-90°C.

[0093] In some implementations, the temperature of the water in step (1) is 70-90°C.

[0094] In some implementations, the soaking time is 0.5-10 hours.

[0095] In some implementations, the soaking time is 0.5-8 hours.

[0096] In some implementations, the soaking time is 1-5 hours.

[0097] In some implementations, the reflux extraction is performed 1-10 times.

[0098] In some implementations, the reflux extraction is performed 2-8 times.

[0099] In some implementations, the reflux extraction is performed 3-6 times.

[0100] In some implementations, the reflux extraction time is 1-10 h / time.

[0101] In some implementations, the reflux extraction time is 2-8 hours per cycle.

[0102] In some implementations, the reflux extraction time is 2-5 hours per cycle.

[0103] In some implementations, the filter used for filtration is a 100-500 mesh filter.

[0104] In some implementations, the filter used for filtration is a 150-300 mesh filter.

[0105] In some embodiments, the adsorption resin is selected from macroporous adsorption resins.

[0106] In some implementations, the elution flow rate is 0.5-8 BV / h.

[0107] In some implementations, the elution flow rate is 1-5 BV / h.

[0108] In some implementations, the elution flow rate is 1-3 BV / h.

[0109] In some embodiments, the gradient elution is performed by sequentially using pure water, 10%-35% v / v ethanol aqueous solution, 36%-55% v / v ethanol aqueous solution, 56%-70% v / v ethanol aqueous solution, 71%-85% v / v ethanol aqueous solution, and 86%-99% v / v ethanol aqueous solution as eluents.

[0110] In some embodiments, the gradient elution is performed by sequentially using pure water, 15%-35% v / v ethanol aqueous solution, 40%-55% v / v ethanol aqueous solution, 60%-70% v / v ethanol aqueous solution, 75%-85% v / v ethanol aqueous solution, and 90%-99% v / v ethanol aqueous solution as eluents.

[0111] In some embodiments, the gradient elution is performed by sequentially using pure water, 30% v / v ethanol aqueous solution, 50% v / v ethanol aqueous solution, 65% v / v ethanol aqueous solution, 80% v / v ethanol aqueous solution and 95% v / v ethanol aqueous solution as eluents.

[0112] In some implementations, the volume of different eluents eluted in the gradient elution is 1-8 BV.

[0113] In some implementations, the volume of different eluents eluted in the gradient elution is 1-5 BV.

[0114] In some implementations, the volume of different eluents eluted in the gradient elution is 2-5 BV.

[0115] In some embodiments, the present invention provides the use of the antioxidant composition in the preparation of skin care products.

[0116] In some implementations, the skincare product includes any one of skincare water, lotion, cream, mask, serum, facial oil, and spray.

[0117] In some implementations, the skincare product is an antioxidant skincare product.

[0118] In some implementations, the skincare product is a skincare product that repairs the skin barrier.

[0119] In some embodiments, the present invention provides the use of the antioxidant composition in the preparation of antioxidants for skin care products.

[0120] In some implementations, the skincare product includes any one of skincare water, lotion, cream, mask, serum, facial oil, and spray.

[0121] In some implementations, the skincare product is an antioxidant skincare product.

[0122] In some implementations, the skincare product is a skincare product that repairs the skin barrier.

[0123] In some embodiments, the present invention provides a skin care product comprising the antioxidant composition.

[0124] In some embodiments, the skin care product includes 0.1-1 wt% of Astragalus membranaceus extract and 0.01-0.5 wt% of Poria cocos extract.

[0125] In some embodiments, the skin care product includes 0.2-0.8 wt% Astragalus membranaceus extract and 0.01-0.25 wt% Poria cocos extract.

[0126] In some embodiments, the skin care product includes 0.3 wt% Astragalus membranaceus extract and 0.02 wt% Poria cocos extract.

[0127] In some embodiments, the skin care product includes 0.05-1 wt% of Paris polyphylla extract and 0.01-0.5 wt% of Poria cocos extract.

[0128] In some embodiments, the skin care product includes 0.05-0.8 wt% of Paris polyphylla extract and 0.01-0.25 wt% of Poria cocos extract.

[0129] In some embodiments, the skin care product includes 0.1 wt% Paris polyphylla extract and 0.04 wt% Poria cocos extract.

[0130] In some embodiments, the skin care product includes 0.05-1 wt% of Paris polyphylla extract and 0.1-1 wt% of Astragalus membranaceus extract.

[0131] In some embodiments, the skin care product includes 0.05-0.8 wt% Paris polyphylla extract and 0.2-0.8 wt% Astragalus membranaceus extract.

[0132] In some implementations, the skincare product includes 0.1 wt% Paris polyphylla extract and 0.6 wt% Astragalus membranaceus extract.

[0133] In some implementations, the skincare product includes any one of skincare water, lotion, cream, mask, serum, facial oil, and spray.

[0134] In some implementations, the skincare product also includes excipients.

[0135] In some embodiments, the excipients include one or more of polyols, emulsifiers, emollients, buffers, chelating agents, thickeners, pH adjusters, skin protectants, preservatives, or antioxidants.

[0136] In some embodiments, the polyol includes one or more of propylene glycol, glycerol, butylene glycol, pentanediol, hexanediol, diglycerol, caprylyl glycol, dipropylene glycol, sorbitol, polyethylene glycol-8, or glycerol polyether-26.

[0137] In some embodiments, the emulsifier includes one or more of hydrogenated lecithin, cetearyl oleate, sorbitan oleate, cetearyl alcohol, or bis-PEG-18 methyl ether dimethylsilane.

[0138] In some embodiments, the emollient includes one or more of squalane, isododecane, isohexadecane, isononyl isononanoate, polydimethylsiloxane, shea butter, caprylic / capric triglyceride, camellia seed oil, or walnut oil.

[0139] In some embodiments, the buffer includes one or more of citric acid, sodium citrate, sodium phosphate, sodium hydrogen phosphate, disodium hydrogen phosphate, or barbiturate.

[0140] In some embodiments, the chelating agent includes one or more of disodium EDTA, tetrasodium EDTA, or sodium hexametaphosphate.

[0141] In some embodiments, the thickener includes one or more of methylcellulose, ethylcellulose, hydroxypropyl methylcellulose, sodium carboxymethylcellulose, sodium polyacrylate, carbomer, acrylate / C10-30 alkanol acrylate crosspolymers, xanthan gum, sclerotinia gum, or sodium polyacrylamide dimethyl taurate.

[0142] In some embodiments, the pH adjuster includes one or more of citric acid, sodium citrate, arginine, sodium hydroxide, triethanolamine, or aminomethylpropanol.

[0143] In some embodiments, the skin protectant includes one or more of sodium hyaluronate, tetrahydromethylpyrimidine carboxylic acid, betaine, acetylglucosamine, β-glucan, acetylated sodium hyaluronate, inositol, or niacinamide.

[0144] In some embodiments, the preservative includes one or more of phenoxyethanol, methylisothiazolinone, benzoic acid, sodium benzoate, benzyl alcohol, chlorphenesin, benzyl alcohol, methylparaben, ethylparaben, or propylparaben.

[0145] In some embodiments, the antioxidant includes one or more of p-hydroxyacetophenone, raspberry ketone, tocopheryl acetate, tocopherol, or ascorbic acid.

[0146] In some embodiments, the present invention provides an antioxidant composition containing 1 mg / mL Poria cocos extract and 15 mg / mL Astragalus membranaceus extract, with a synergistic fraction as high as 23.03, exhibiting synergistic antioxidant activity.

[0147] In some implementations, when the mass ratio of the Poria cocos extract to the Astragalus membranaceus extract is 1:15, the synergistic score obtained is higher than when the mass ratios are 1:60, 1:30, 1:7.5, and 1:3.75. This shows that the synergistic antioxidant effect obtained by the combination of Poria cocos extract and Astragalus membranaceus extract with a specific mass ratio is beyond expectations.

[0148] In some embodiments, the present invention provides an antioxidant composition containing 1 mg / mL Poria cocos extract and 2.5 mg / mL Paris polyphylla extract, with a synergistic fraction of 12.906, exhibiting synergistic antioxidant activity.

[0149] In some implementations, when the mass ratio of the Paris polyphylla extract to the Poria cocos extract is 2.5:1, the synergistic score obtained is higher than when the mass ratios are 10:1, 5:1, 1.25:1, and 0.625:1. This shows that the synergistic antioxidant effect obtained by the combination of Paris polyphylla extract and Poria cocos extract with a specific mass ratio is beyond expectations.

[0150] In some embodiments, the present invention provides an antioxidant composition containing 15 mg / mL Astragalus membranaceus extract and 2.5 mg / mL Paris polyphylla extract, with a synergistic fraction as high as 15.10, exhibiting synergistic antioxidant activity.

[0151] In some implementations, when the mass ratio of the Paris polyphylla extract to the Astragalus membranaceus extract is 1:6, the synergistic score obtained is higher than when the mass ratios are 1:24, 1:12, and 1:3. This shows that the synergistic antioxidant effect obtained by the combination of Paris polyphylla extract and Astragalus membranaceus extract with a specific mass ratio is beyond expectations.

[0152] In some embodiments, the present invention provides a skin care essence containing a combination of Poria cocos extract and Astragalus membranaceus extract, which can effectively resist oxidation, reduce skin moisture loss, and repair the skin barrier.

[0153] In some embodiments, the present invention provides a skin care essence containing a combination of Paris polyphylla extract and Poria cocos extract, which can effectively resist oxidation, reduce skin moisture loss, and repair the skin barrier.

[0154] In some embodiments, the present invention provides a skin care cream containing a combination of Paris polyphylla extract and Astragalus membranaceus extract, which can effectively resist oxidation, reduce skin moisture loss, and repair the skin barrier.

[0155] The ZIP model assesses the effects of a combination of two extracts under the assumption that each extract does not affect the efficacy of the other. The Bliss model assumes that the effects of the extracts are independent and calculates the expected combined effect based on the probability of independent effects. The HSA model defines a synergistic effect as a combined response that is greater than the response of either extract alone. Different models analyze the effects of extract combinations based on different assumptions and methods, thus providing a comprehensive understanding of extract interactions. Positive or negative synergistic scores represent synergistic and antagonistic effects, respectively. Attached Figure Description

[0156] Figure 1 The results show the DPPH free radical scavenging rate of Astragalus membranaceus extract.

[0157] Figure 2 The results show the DPPH free radical scavenging rate of Poria cocos extract.

[0158] Figure 3This is a ZIP model illustrating the synergistic effect of the combination of Poria cocos extract and Astragalus membranaceus extract in scavenging DPPH free radicals.

[0159] Figure 4 This is a diagram showing the synergistic effect of the combination of Poria cocos extract and Astragalus membranaceus extract in scavenging DPPH free radicals (Bliss model).

[0160] Figure 5 The results show the DPPH free radical scavenging rate of Paris polyphylla extract.

[0161] Figure 6 This is a ZIP model illustrating the synergistic effect of the combination of Poria cocos extract and Paris polyphylla extract in scavenging DPPH free radicals.

[0162] Figure 7 This is a ZIP model illustrating the synergistic effect of Astragalus membranaceus extract and Paris polyphylla extract in scavenging DPPH free radicals.

[0163] Figure 8 This is a diagram showing the synergistic effect of Astragalus membranaceus extract and Paris polyphylla extract in scavenging DPPH free radicals (Bliss model).

[0164] Figure 9 The results of the VISIA-CR red area percentage test for Essence I containing a combination of Poria cocos extract and Astragalus membranaceus extract.

[0165] Figure 10 The results of the VISIA-CR red zone a* value test for Essence II containing a combination of Poria cocos extract and Paris polyphylla extract.

[0166] Figure 11 The TEWL value test results are for a face cream containing a combination of Astragalus membranaceus extract and Paris polyphylla extract. Detailed Implementation

[0167] The following specific embodiments further illustrate the technical solution of the present invention. These specific embodiments do not represent a limitation on the scope of protection of the present invention. Non-essential modifications and adjustments made by others based on the concept of the present invention still fall within the scope of protection of the present invention.

[0168] Unless otherwise specified, the experimental methods in the following examples all employ conventional techniques in this technical field or follow the conditions recommended by the manufacturer; reagents or instruments whose manufacturers are not specified are all commercially available products.

[0169] Unless otherwise defined, all technical and scientific terms used herein are defined identically to those familiar to those skilled in the art. Furthermore, any methods and materials similar to or equivalent to those described herein may be applied to the methods of this invention, with preferred methods and materials described in the detailed embodiments.

[0170] In this specification, the range of values ​​referred to as “value A - value B” is the range that includes the endpoint values ​​A and B.

[0171] In this article, the antioxidant composition comprises the following components in parts by weight: 7.5 to 30 parts of Astragalus membranaceus extract and 0.5 to 2 parts of Poria cocos extract. It can be understood that the weight parts of Astragalus membranaceus extract can be any value between 7.5 and 30; for example, the weight parts of Astragalus membranaceus extract can be 19, 19.5, 28, or 28.5. Similarly, the weight parts of Poria cocos extract can be any value between 0.5 and 2; for example, the weight parts of Poria cocos extract can be 0.65, 0.75, 1.85, or 1.95.

[0172] In this article, the antioxidant composition comprises the following components in parts by weight: 1.25-5 parts of Paris polyphylla extract and 0.5-2 parts of Poria cocos extract. This can be understood as the weight percentage of Paris polyphylla extract being any value between 1.25 and 5; for example, the weight percentage of Paris polyphylla extract could be 1.35, 1.45, 4.5, or 4.75. Similarly, the weight percentage of Poria cocos extract could be any value between 0.5 and 2; for example, the weight percentage of Poria cocos extract could be 0.75, 0.85, 1.5, or 1.75.

[0173] In this document, the antioxidant composition comprises the following components in parts by weight: 1.25–5 parts of Paris polyphylla extract and 7.5–30 parts of Astragalus membranaceus extract. This can be understood as the weight percentage of Paris polyphylla extract being any value between 1.25 and 5; for example, the weight percentage of Paris polyphylla extract could be 1.35, 1.45, 4.5, or 4.75. Similarly, the weight percentage of Astragalus membranaceus extract could be any value between 7.5 and 30; for example, the weight percentage of Astragalus membranaceus extract could be 7.75, 10.5, 28.5, or 29.5.

[0174] All other specific point values ​​not listed above within the numerical ranges mentioned above can be selected and are all within the protection scope of this invention. For the sake of brevity, they will not be described in detail here.

[0175] In this article, IC50 is the concentration of the extract when the DPPH free radical scavenging rate reaches 50%.

[0176] In this article, the Astragalus extract is an extract from the dried root of Astragalus membranaceus (Fisch.) Bge., a plant belonging to the genus Astragalus in the family Leguminosae.

[0177] In this article, the extract of Paris fargesii is the extract of the dried rhizome of Paris fargesii Franch., a plant belonging to the genus Paris of the family Veratraceae.

[0178] In this article, the Poria cocos extract is an extract from the dried sclerotium of the fungus Poria cocos (Schw.) Wolf, belonging to the Polyporaceae family.

[0179] In this article, Astragalus membranaceus, Poria cocos, and Paris polyphylla were purchased from Yunnan Yicao Yiwei Traditional Chinese Medicine Industry Co., Ltd.

[0180] In this study, 2,2-diphenyl-1-picrylhydrazine (DPPH, CAS No.: 1898-66-4) was purchased from Beyotime Biotechnology Co., Ltd.; methanol (CAS No.: 67-56-1), NaH2PO4·2H2O, Na2HPO4·12H2O, sulfonamide (CAS: 63-74-1), concentrated phosphoric acid (CAS: 7664-38-2), N-(1-naphthyl)ethylenediamine hydrochloride (CAS: 1465-25-4), and 2,2′-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, CAS No.: 30931-67-0) were purchased from Sinopharm Chemical Reagent Co., Ltd.; potassium persulfate (CAS No.: 7727-21-1) was purchased from Inokai; and sodium nitroprusside (SNP, CAS: 13755-38-9) was purchased from Thermo Fisher Scientific.Sodium hyaluronate (CAS No.: 9067-32-7) was purchased from Shandong Tiansheng Biotechnology Co., Ltd.; disodium EDTA (CAS No.: 6381-92-6) was purchased from Shantou Dian Pharmaceutical Co., Ltd.; raspberry ketone (CAS: 5471-51-2) was purchased from Guangzhou Wanlu Chemical Co., Ltd.; acrylate / C10-30 alkanol acrylate crosspolymer was purchased from Guangzhou Zhaoheng Trade Development Co., Ltd.; phenoxyethanol (CAS: 122-99-6) was purchased from Guangzhou Wanlu Chemical Co., Ltd.; hydrogenated lecithin (CAS: 92128-87-5) was purchased from Guangzhou Rongdao Chemical Co., Ltd.; betaine (CAS: 107-43-7) was purchased from the Guangzhou branch of KONEO Trading (Shanghai) Co., Ltd.; and bis-PEG-18... Methyl ether dimethylsilane was purchased from Guangzhou Wanlu Chemical Co., Ltd.; glycerol (CAS: 56-81-5) was purchased from Guangdong Hongshi Import & Export Co., Ltd.; butanediol (CAS: 19132-06-0) was purchased from Shanghai Huiyi Trading Co., Ltd.; squalane (CAS: 111-01-3) was purchased from Zhuoke Biotechnology (Hainan) Co., Ltd.; caprylic / capric triglyceride (CAS: 65381-09-1) was purchased from Shanghai Youwen Chemical Co., Ltd.; tocopheryl acetate (CAS: 7695-91-2) was purchased from Guangzhou Kesa Road Trading Co., Ltd.; acetylated sodium hyaluronate (CAS: Purchased from Shandong Tiansheng Biotechnology Co., Ltd.; Tetrahydromethylpyrimidine carboxylic acid (CAS: 96702-03-3) from Guangzhou Hengtao Trading Co., Ltd.; Arginine (CAS: 74-79-3) from Nanjing Lizhihong Biotechnology Co., Ltd.; Daily fragrance from Man's (China) Fragrance & Flavor Co., Ltd.; Glyceryl polyether-26 (CAS: 74-79-3) from Guangzhou Wanlu Chemical Co., Ltd.; Inositol (CAS: 87-89-8) from Guangzhou Branch of KONEO Trading (Shanghai) Co., Ltd.; 1,2-Hexanediol (CAS: 6920-22-5) from Guangzhou Rongdao Chemical Co., Ltd.; Sodium polyacrylamide dimethyl taurate from Shanghai Baihao Bosheng. The following products were purchased from Guangzhou Wanlu Chemical Co., Ltd.: cetearyl alcohol oleate, sorbitan oleate, cetearyl alcohol (CAS: 4390-04-9), isocetearyl alcohol (CAS: 67762-27-0), isononyl isononanoate (CAS: 42131-25-9), polydimethylsiloxane (CAS: 9016-00-6), camellia seed oil, and shea butter from Kunming Yibo Technology Co., Ltd.

[0181] Example 1: Synergistic effect analysis of the combination of Poria cocos extract and Astragalus membranaceus extract I. DPPH free radical scavenging ability test of individual raw materials 1. Experimental Materials and Methods: 1.1 Extraction method of Astragalus membranaceus extract: (1) Weigh 40 kg of Astragalus membranaceus coarse powder after passing through a 10-mesh sieve, soak it in 10 L of 8wt% ammonia water at room temperature (25℃) for 20 h; reflux extract for 4 h, extract 3 times, add 15 L of 8wt% ammonia water for the last two reflux extracts, and combine to obtain the extract; (2) Concentrate the extract obtained in step (1) into a paste: the mass ratio of the extract is 1:1.01, the temperature is 30℃, and the paste of Astragalus extract is obtained. Dry it to obtain Astragalus extract.

[0182] 1.2 Extraction method of Poria cocos extract: (1) Soak 1 kg of Poria cocos powder in 10 L of 80℃ water for 2 h, then heat to 90℃ for reflux extraction 3 times, 2 h each time. Add 10 L of water to the last two reflux extractions and combine to obtain the extract. (2) Filter the extract obtained in step (1) through a 200-mesh filter and concentrate it to obtain a concentrated solution; (3) Load the concentrated solution obtained in step (2) onto an 80L macroporous adsorption resin (model D101), and perform gradient elution with pure water, 30% (v / v), 50% (v / v), 65% (v / v), 80% (v / v), and 95% (v / v) ethanol aqueous solution in sequence, eluting 4 BV each time, with a flow rate of 2 BV / h, and collect the eluent; (4) Concentrate and dry the eluent obtained in step (3) to obtain Poria cocos extract.

[0183] 1.3 DPPH free radical scavenging rate detection method: Solution preparation: DPPH solution: Weigh 10 mg of DPPH solid and dissolve it in 50 mL of methanol. Dilute the solution with methanol until the absorbance at 517 nm is 0.8.

[0184] Sample preparation: Take the Poria cocos extract and Astragalus membranaceus extract respectively, and prepare the sample solutions to be tested using methanol as solvent.

[0185] The experimental steps are as follows: (1) As shown in Table 1, add the reagents according to the table below and mix well; (2) React at room temperature in the dark for 30 min; (3) Centrifuge at 10,000 rpm for 3 min, and take 200 μL of supernatant into a 96-well plate; (4) Detect absorbance at 517 nm.

[0186] Table 1 (5) Calculation formula: DPPH radical inhibition rate % = [1 - (As - Asb) / (Ac - Acb)] × 100% In the formula: As: Absorbance of the sample group; Asb: Absorbance of the blank sample group; Ac: Absorbance of the control group; Acb: Absorbance of the blank control group.

[0187] 2. Experimental Results (1) The results of DPPH free radical scavenging rate of Astragalus extract are shown in Figure 1 Its IC50 is 23.75 mg / mL; (2) The results of DPPH free radical scavenging rate of Poria cocos extract are shown in the figure. Figure 2 Its IC50 is 2.21 mg / mL.

[0188] II. Synergistic Effect Analysis of the Composition 1. Experimental Methods The Poria cocos extract and the Astragalus membranaceus extract were dissolved in methanol and prepared into compositions with different ratios (as shown in Table 2). The in vitro antioxidant activity of the compositions was determined by the DPPH free radical scavenging method, and the detection method was the same as in 1.3.

[0189] After obtaining the DPPH radical scavenging rate data of the composition, the synergistic effect of the combined application of the composition was further evaluated using Synergy Finder software. The corresponding ZIP Synergy Score and Bliss Synergy Score were calculated based on the ZIP model (Reference: Yadav B, Wennerberg K, Aittokallio T, et al. Searching for drug synergy in complex dose–response landscapes using an interaction potency model[J]. Computational and structural biotechnology journal, 2015, 13: 504-513.) and the Bliss model (Reference: Bliss C I. The toxicity of poisons applied jointly 1[J]. Annals of appliedbiology, 1939, 26(3): 585-615.) to evaluate the synergistic properties of the composition.

[0190] The ZIP Synergy Score is a Zero Interaction Potency Model (ZIP) used to assess the strength of synergistic effects between two or more factors (variables). It measures the strength of the synergistic effect by calculating the expected combined effect and comparing the observed effect with the expected effect. The synergistic effect score (ZIP Synergy Score value) and the resulting matrix plot can display the interactions between drugs. ZIP Synergy Score values ​​>0, =0, and <0 indicate synergistic (red), additive (white), and antagonistic (green) effects, respectively. Score values ​​<-10 or >10 indicate strong antagonistic or strong synergistic effects; the darker the color, the stronger the synergistic or antagonistic effect.

[0191] The Bliss Synergy Score is an index based on the Bliss Independence Model to assess the synergistic effect of drug combinations. It measures the strength of the synergistic effect by comparing the predicted synergistic effect (based on probability product theory) with the observed synergistic effect when each drug acts independently. The Bliss Synergy Score and the resulting matrix visually display the interactions between drugs. A Bliss Synergy Score value >0, =0, and <0 indicate a synergistic (red), additive (white), and antagonistic (green) effect, respectively. Generally, a Score value <-10 or >10 is considered a strong antagonistic or strong synergistic effect; the darker the color, the stronger the synergistic or antagonistic effect.

[0192] 2. Experimental Results (1) Based on the IC50 results of the DPPH scavenging rate test of each single raw material, different ratios were compounded and the DPPH free radical scavenging rate of the composition under different ratios was measured. The results are shown in Table 2.

[0193] Table 2 DPPH free radical scavenging rate of the combination of Astragalus membranaceus extract and Poria cocos extract (2) The DPPH free radical scavenging rate data of the compositions with different ratios were further analyzed using the ZIP model algorithm with Synergy Finder software, and the synergistic effect was evaluated with the ZIP Synergy Score. The results are as follows: Figure 3 As shown, the synergistic effect diagram of the combination of Poria cocos extract and Astragalus membranaceus extract is red in the range of Poria cocos extract: Astragalus membranaceus extract = 0.5~2: 7.5~30 mg / mL, and the mean ZIP Synergy Score in the figure is >10, indicating that the two have a strong synergistic antioxidant effect.

[0194] The synergistic score results are shown in Table 3. It can be seen that when the mass ratio of Poria cocos extract to Astragalus membranaceus extract is 1:15, the synergistic score is the highest, and the synergistic antioxidant effect of the composition is the best.

[0195] Table 3. δ fraction of the combination of Astragalus membranaceus extract and Poria cocos extract (3) The DPPH free radical scavenging rate data of the compositions under different ratios were further analyzed using the Bliss model algorithm with Synergy Finder software, and the synergistic effect was evaluated with the Bliss Synergy Score. The results are as follows: Figure 4As shown, the synergistic effect diagram of the combination of Poria cocos extract and Astragalus membranaceus extract is red in the range of Poria cocos extract: Astragalus membranaceus extract = 0.5~2: 7.5~30 mg / mL, and the mean Bliss Synergy Score in the figure is >10, indicating that the two have a strong synergistic antioxidant effect.

[0196] The synergistic score results are shown in Table 4. It can be seen that when the mass ratio of Poria cocos extract to Astragalus membranaceus extract is 1:15, the synergistic score is the highest, and the synergistic antioxidant effect of the composition is the best.

[0197] Table 4. δ fraction of the combination of Astragalus membranaceus extract and Poria cocos extract III. Detection of the ABTS free radical scavenging ability of the composition 1. Experimental Methods: 1.1 The Poria cocos extract and the Astragalus membranaceus extract were dissolved in methanol and prepared into a composition. The in vitro antioxidant activity of the composition was determined by the ABTS free radical scavenging method.

[0198] 1.2 ABTS radical scavenging rate detection method: Preparation of ABTS solution: Weigh 6.6 mg of potassium persulfate and dissolve it in 10 mL of deionized water to prepare a 2.45 mM potassium persulfate solution. Weigh 7.7 mg of ABTS and dissolve it in 2 mL of potassium persulfate solution. After reacting for 16 h, a 7 mM concentrated ABTS solution is obtained. Dilute it with deionized water until the absorbance at 734 nm is 0.8 ± 0.02 to obtain the ABTS solution.

[0199] Sample solution preparation: Take the Poria cocos extract and Astragalus membranaceus extract respectively, and use methanol as solvent to prepare the sample solution to be tested.

[0200] The experimental steps are as follows: (1) As shown in Table 5, add the reagents according to the table below and mix well; (2) React at room temperature in the dark for 10 min; (3) Centrifuge at 10,000 rpm for 3 min, and take 200 μL of supernatant into a 96-well plate; (4) Detect absorbance at 734 nm.

[0201] (5) Calculate the ABTS radical scavenging rate of the sample according to the following formula.

[0202] Table 5 Calculation formula: ABTS free radical scavenging rate (%) = (1-A) A / AB )×100% In the formula: A A The absorbance of sample A in sample group A is... B The absorbance is that of control sample B.

[0203] 2. Experimental Results: The ABTS free radical scavenging rate of the combination of Poria cocos extract and Astragalus membranaceus extract is shown in Table 6, indicating that the combination of Astragalus membranaceus extract and Astragalus membranaceus extract has antioxidant effects.

[0204] Table 6. ABTS free radical scavenging rate of the combination of Poria cocos extract and Astragalus membranaceus extract IV. Detection of the NO radical scavenging ability of the composition 1. Experimental Methods: 1.1 The Poria cocos extract and the Astragalus membranaceus extract were dissolved in 70% ethanol and prepared into a composition. The in vitro antioxidant activity of the composition was determined by the NO free radical scavenging method.

[0205] 1.2 Method for detecting NO free radical scavenging rate: Solution preparation: Griess Reagent A: Weigh 100 mg of sulfonamide, add 200 μL of 85% concentrated phosphoric acid, and add water to 10 mL; Griess Reagent B: Weigh 10 mg of N-(1-naphthyl)ethylenediamine hydrochloride and add water to 10 mL; 0.2 M PBS (pH 7.4): Weigh 3.1192 g of NaH2PO4·2H2O and dissolve it in 100 mL of water, weigh 7.1628 g of Na2HPO4·12H2O and dissolve it in 100 mL of water, and adjust the pH to 7.4; 0.5 mM SNP solution: Weigh 2.9775 mg of SNP and dissolve it in 1 mL of 0.2 M PBS solution at pH 7.4 to obtain a 10 mM SNP solution. Then further dilute it to 0.5 mM using 0.2 M PBS solution at pH 7.4.

[0206] Sample preparation: Take the Poria cocos extract and Astragalus membranaceus extract respectively, and use 70% ethanol as solvent to prepare test sample solutions of different concentrations.

[0207] The experimental steps are as follows: (1) As shown in Table 7, mix 200 μL of 0.5 mmol / L SNP solution and 200 μL of sample solution, and irradiate under UVB in an ultraviolet crosslinker for 60 min. (2) First add 200 μL of Griess reagent A, and use an ELISA reader to detect the absorbance at 550 nm. This is the blank absorbance value. (3) Add 200 μL of Griess reagent B and react in the dark for 10 min; (4) The absorbance at 550 nm was detected by an enzyme-linked immunosorbent assay (ELISA) reader.

[0208] Table 7 (5) Calculation formula: NO clearance rate (%) = [1 - (As - Asb) / (Ac - Acb)] × 100% Where: As is the absorbance value of the sample group; A c α is the absorbance value of the control group; β is the blank absorbance value of the sample group; α is the blank absorbance value of the control group.

[0209] 2. Experimental Results The NO free radical scavenging rate of the combination of Poria cocos extract and Astragalus membranaceus extract is shown in Table 8, indicating that the combination of Poria cocos extract and Astragalus membranaceus extract has a strong antioxidant effect.

[0210] Table 8. NO radical scavenging rate of the combination of Poria cocos extract and Astragalus membranaceus extract Example 2 Preparation of a combination of Poria cocos extract and Astragalus membranaceus extract A composition with skin antioxidant activity is provided, wherein the composition is prepared by mixing a liposome pre-dispersion of Astragalus membranaceus extract with an aqueous solution of Poria cocos extract, wherein the mass ratio of Poria cocos extract to Astragalus membranaceus extract in the composition is 1:15. The composition contains 0.2% (w / w) Poria cocos extract and 3% (w / w) Astragalus membranaceus extract.

[0211] The Poria cocos extract and Astragalus membranaceus extract are the same as in Example 1. The steps for preparing the liposome predispersant from the Astragalus membranaceus extract are as follows: Weigh 8% (w / w) glycerol, 3% (w / w) PEG-40 hydrogenated castor oil, 2% (w / w) phytosterol / octyldodecyl lauroyl glutamate, 6% (w / w) Astragalus membranaceus extract, and add the remainder to deionized water to 100% (w / w). After heating to 80°C, homogenize under high pressure microfluidic jet at a homogenization pressure of 1500 bar to obtain the liposome predispersant.

[0212] Example 3: Synergistic effect analysis of the combination of Poria cocos extract and Paris polyphylla extract I. DPPH free radical scavenging ability test of individual raw materials 1. Experimental Materials and Methods 1.1 The extraction method of Poria cocos extract is the same as in Example 1.

[0213] 1.2 The extraction method of Paris polyphylla extract refers to the published patent application (Yunnan Baiyao Group Shanghai Technology Co., Ltd., Yunnan Baiyao Group Health Products Co., Ltd. A Paris polyphylla extract, its preparation method and application: CN202310462046.4[P]. 2024-01-05.), as follows: (1) 25.0 kg of dried Paris rhizomes were crushed using a coarse crusher (model CSJ-60) with a 4 mm mesh. 20.0 kg of the coarse Paris powder was weighed and put into a 300 L multi-functional extraction tank (model TQ-SH). 240 L of 70% (v / v) ethanol aqueous solution was added and soaked for 30 minutes. The mixture was then refluxed and extracted 3 times, 2 hours each time. 240 L of 70% (v / v) ethanol aqueous solution was added for the last two reflux extractions and the extracts were combined.

[0214] (2) Filter the extract from step (1) through a 200-mesh filter. Concentrate the filtrate to 17L under reduced pressure using a 230L single-effect external circulation evaporator (model WZ) to obtain a concentrated solution.

[0215] (3) After cooling the concentrate from step (2), add it to an 80L macroporous adsorption resin (model D101) that has been treated with 95% (v / v) ethanol aqueous solution and then equilibrated with pure water. The loading flow rate is 1.5 BV / h. Elute sequentially with pure water, 30% (v / v), 50% (v / v), 65% (v / v), 80% (v / v), and 95% (v / v) ethanol aqueous solution, each for 2 BV, at a flow rate of 2 BV / h. Collect the eluent.

[0216] (4) The eluent from step (3) was concentrated under reduced pressure and then vacuum dried (model YZG-SH) to obtain Paris polyphylla extract.

[0217] 1.3 The method for detecting DPPH free radical scavenging ability is the same as in Example 1.

[0218] 2. Experimental Results: (1) The results of DPPH free radical scavenging rate of Poria cocos extract are shown in the figure. Figure 2 Its IC50 is 2.21 mg / mL; (2) The DPPH free radical scavenging rate results of the Paris polyphylla extract are shown in the figure. Figure 5 Its IC50 is 6.88 mg / mL.

[0219] II. Synergistic Effect Analysis of the Composition 1. Experimental Methods The Poria cocos extract and the Paris polyphylla extract were dissolved in methanol and prepared into compositions with different ratios as shown in Table 9. The in vitro antioxidant activity of the compositions was determined by the DPPH free radical scavenging method, and the detection method was the same as in Example 1.

[0220] After obtaining the DPPH radical scavenging rate data of the composition, the synergistic effect of the combined application of the composition was further evaluated using Synergy Finder software. The corresponding ZIP Synergy Score and HSA Synergy Score were calculated based on the ZIP model (Reference: Yadav B, Wennerberg K, Aittokallio T, et al. Searching for drug synergy in complex dose–response landscapes using an interaction potency model[J]. Computational and structural biotechnology journal, 2015, 13: 504-513.) and the HSA model (Reference: Berenbaum MC. What is synergy?[J]. Pharmacological reviews, 1989, 41(2): 93-141.) to evaluate the synergistic properties of the composition.

[0221] The HSA Synergy Score is an indicator used to assess the synergistic effect of drug combinations based on the Highest Single Agent Model (HSA). It measures the strength of the synergistic effect by comparing the observed effect of the combination with the maximum effect of each individual drug. The HSA synergy score and the resulting matrix visually display the interactions between drugs. HSA Synergy Score values ​​>0, =0, and <0 indicate synergistic (red), additive (white), and antagonistic (green) effects, respectively. Generally, a score <-10 or >10 is considered a strong antagonistic or strong synergistic effect; the darker the color, the stronger the synergistic or antagonistic effect.

[0222] 2. Experimental Results (1) Based on the IC50 results of the DPPH scavenging rate test of each single raw material, different ratios were compounded and the DPPH free radical scavenging rate of the composition under different ratios was measured. The results are shown in Table 9.

[0223] Table 9. DPPH free radical scavenging rate of the combination of Poria cocos extract and Paris polyphylla extract (2) The DPPH free radical scavenging rate data of the compositions with different ratios were further analyzed using the ZIP model algorithm with Synergy Finder software, and the synergistic effect was evaluated with the ZIP Synergy Score. The results are as follows: Figure 6 As shown, the combination of Poria cocos extract and Paris polyphylla extract showed a red color in the range of Poria cocos extract: Paris polyphylla extract = 0.5~2:1.25~5 mg / mL, and the mean ZIP Synergy Score in the figure was >0, indicating that the two have a synergistic antioxidant effect.

[0224] (3) The DPPH free radical scavenging rate data of the compositions under different ratios were further analyzed by the HSA model algorithm using Synergy Finder software, and the synergistic effect was evaluated by HSA Synergy Score. The results are shown in Table 10.

[0225] Table 10 δ fraction of the combination of Poria cocos extract and Paris polyphylla extract As shown in Table 10, the highest synergistic score was obtained when the mass ratio of Paris polyphylla extract to Poria cocos extract was 2.5:1. This indicates that the composition exhibits a stronger synergistic antioxidant effect at this ratio.

[0226] III. Detection of the ABTS free radical scavenging ability of the composition 1. Experimental Methods: 1.1 The Poria cocos extract and the Paris polyphylla extract were dissolved in methanol and prepared into the composition shown in Table 11. The in vitro antioxidant activity of the composition was determined by the ABTS free radical scavenging method.

[0227] 1.2 The method for detecting ABTS free radical scavenging rate is the same as in Example 1.

[0228] 2. Experimental Results: The ABTS free radical scavenging rate of the combination of Poria cocos extract and Paris polyphylla extract is shown in Table 11, indicating that the combination of Poria cocos extract and Paris polyphylla extract has a strong antioxidant effect.

[0229] Table 11 ABTS free radical scavenging rate of the combination of Poria cocos extract and Paris polyphylla extract IV. Detection of the NO radical scavenging ability of the composition 1. Experimental Methods: 1.1 The Poria cocos extract and the Paris polyphylla extract were dissolved in 70% ethanol and prepared into the composition shown in Table 12. The in vitro antioxidant activity of the composition was determined by the NO free radical scavenging method.

[0230] 1.2 The method for detecting NO free radical scavenging rate is the same as in Example 1.

[0231] 2. Experimental Results The NO free radical scavenging rate of the combination of Poria cocos extract and Paris polyphylla extract is shown in Table 12, indicating that the combination of Poria cocos extract and Paris polyphylla extract has antioxidant effect.

[0232] Table 12 NO radical scavenging rate of the combination of Poria cocos extract and Paris polyphylla extract Example 4: Preparation of a combination of Poria cocos extract and Paris polyphylla extract A composition with skin antioxidant properties, wherein the composition is a water-based solvent and is composed of the following mass ratio: Poria cocos extract: Paris polyphylla extract = 2:5. The Poria cocos extract is the same as in Example 1, and the Paris polyphylla extract is the same as in Example 3. The composition comprises 0.4% (w / w) Poria cocos extract and 1% (w / w) Paris polyphylla extract.

[0233] Example 5: Synergistic effect analysis of the combination of Astragalus membranaceus extract and Paris polyphylla extract I. DPPH free radical scavenging ability test of individual raw materials 1. Experimental Materials and Methods 1.1 The extraction method of Astragalus membranaceus extract is the same as in Example 1.

[0234] 1.2 The extraction method of Paris polyphylla extract is the same as in Example 3.

[0235] 1.3 The method for detecting DPPH free radical scavenging ability is the same as in Example 1.

[0236] 2. Experimental Results (1) The results of DPPH free radical scavenging rate of Astragalus extract are shown in Figure 1 Its IC50 is 23.75 mg / mL; (2) The DPPH free radical scavenging rate results of the Paris polyphylla extract are shown in the figure. Figure 5 Its IC50 is 6.88 mg / mL.

[0237] II. Synergistic Effect Analysis of the Composition 1. Experimental Methods The Astragalus extract and the Paris polyphylla extract were dissolved in methanol and prepared into compositions with different ratios as shown in Table 13. The in vitro antioxidant activity of the compositions was determined by the DPPH free radical scavenging method, and the detection method was the same as in Example 1.

[0238] After obtaining the DPPH radical scavenging rate data of the composition, the Synergy Finder software was used to further evaluate the synergistic effect of the combined application of the composition. The ZIP Synergy Score and Bliss Synergy Score were used to evaluate the synergistic effect, as in Example 1.

[0239] 2. Experimental Results (1) Based on the IC50 results of the DPPH scavenging rate test of each single raw material, different ratios were compounded and the DPPH free radical scavenging rate of the composition under different ratios was measured. The results are shown in Table 13.

[0240] Table 13 DPPH free radical scavenging rate of the combination of Astragalus extract and Paris polyphylla extract (2) The DPPH free radical scavenging rate data of the compositions with different ratios were further analyzed using the ZIP model algorithm with Synergy Finder software, and the synergistic effect was evaluated with the ZIP Synergy Score. The results are as follows: Figure 7 As shown, the combination of Astragalus membranaceus extract and Paris polyphylla extract showed a red color in the range of Astragalus membranaceus extract: Paris polyphylla extract = 7.5~30: 1.25~5 mg / mL, and the average ZIP Synergy Score in the figure was >10, indicating that the two have a strong synergistic antioxidant effect.

[0241] The synergistic score results are shown in Table 14. It can be seen that when the mass ratio of Paris polyphylla extract to Astragalus membranaceus extract is 1:6, the synergistic score is the highest, and the synergistic antioxidant effect of the composition is the best.

[0242] Table 14 δ fraction of the combination of Paris polyphylla extract and Astragalus membranaceus extract (3) The DPPH free radical scavenging rate data of the compositions under different ratios were further analyzed using the Bliss model algorithm with Synergy Finder software, and the synergistic effect was evaluated with the Bliss Synergy Score. The results are as follows: Figure 8As shown, the synergistic effect diagram of the combination of Paris polyphylla extract and Astragalus membranaceus extract is red in the range of Paris polyphylla extract: Astragalus membranaceus extract = 1.25~5: 7.5~30 mg / mL, and the mean Bliss Synergy Score in the figure is >10, indicating that the two have a strong synergistic antioxidant effect.

[0243] The synergistic score results are shown in Table 15. It can be seen that when the mass ratio of Paris polyphylla extract to Astragalus membranaceus extract is 1:6, the synergistic score is the highest, and the synergistic antioxidant effect of the composition is the best.

[0244] Table 15 δ fraction of the combination of Paris polyphylla extract and Astragalus membranaceus extract III. Detection of the ABTS free radical scavenging ability of the composition 1. Experimental Methods: 1.1 The Astragalus membranaceus extract and the Paris polyphylla extract were dissolved in methanol and prepared into the composition shown in Table 16. The in vitro antioxidant activity of the composition was determined by the ABTS free radical scavenging method.

[0245] 1.2 The method for detecting ABTS free radical scavenging rate is the same as in Example 1.

[0246] 2. Experimental Results: The ABTS free radical scavenging rate of the combination of Astragalus membranaceus extract and Paris polyphylla extract is shown in Table 16, indicating that the combination of Astragalus membranaceus extract and Paris polyphylla extract has antioxidant activity.

[0247] Table 16 ABTS radical scavenging rate of the combination of Astragalus membranaceus extract and Paris polyphylla extract IV. Detection of the NO radical scavenging ability of the composition 1. Experimental Methods: 1.1 The Astragalus extract and the Paris polyphylla extract were dissolved in 70% ethanol and prepared into the composition shown in Table 17. The in vitro antioxidant activity of the composition was determined by the NO free radical scavenging method.

[0248] 1.2 The method for detecting NO free radical scavenging rate is the same as in Example 1.

[0249] 2. Experimental Results The NO free radical scavenging rate of the combination of Astragalus membranaceus extract and Paris polyphylla extract is shown in Table 17, indicating that the combination of Astragalus membranaceus extract and Paris polyphylla extract has antioxidant effect.

[0250] Table 17 NO radical scavenging rate of the combination of Astragalus membranaceus extract and Paris polyphylla extract Example 6 Preparation of a composition of Astragalus membranaceus extract and Paris polyphylla extract A composition with skin antioxidant properties is disclosed, comprising a liposome predispersant prepared from Astragalus membranaceus extract and mixed with an aqueous solution of Paris polyphylla extract. The mass ratio of Astragalus membranaceus extract to Paris polyphylla extract in the composition is 6:1. The composition contains 6% (w / w) Astragalus membranaceus extract and 1% (w / w) Paris polyphylla extract. The Astragalus membranaceus extract is the same as in Example 1, and the Paris polyphylla extract is the same as in Example 3. The method for preparing the liposome predispersant from Astragalus membranaceus extract is the same as in Example 2.

[0251] Example 7: Skin care application of a combination of Poria cocos extract and Astragalus membranaceus extract. I. Preparation of the essence containing the composition of Example 2 1. Weigh 0.3% (w / w) sodium hyaluronate, 0.05% (w / w) disodium EDTA, 0.4% (w / w) raspberry ketone, 0.5% (w / w) acrylate / C10-30 alkanol acrylate crosspolymer, 0.5% (w / w) phenoxyethanol, 1% (w / w) hydrogenated lecithin, 1% (w / w) betaine, 1% (w / w) bis-PEG-18 methyl ether dimethylsilane, 4% (w / w) glycerol, 6% (w / w) butanediol, and 10% (w / w) deionized water, add them to the main pot, stir and disperse evenly to obtain component I.

[0252] 2. Weigh 2% (w / w) squalane, 2% (w / w) caprylic / capric triglycerides, 0.5% (w / w) tocopheryl acetate, and 0.02% (w / w) daily fragrance. Add 10% (w / w) deionized water to obtain component II. Heat the mixture to 80°C, pre-mix and homogenize it thoroughly, then cool it to 37°C. Add component II to the main pot and mix thoroughly.

[0253] 3. Weigh 0.1% (w / w) acetylated sodium hyaluronate, 0.5% (w / w) tetrahydromethylpyrimidine carboxylic acid, 0.1% (w / w) the composition of Example 2 and 5% (w / w) deionized water, disperse them evenly to obtain the raw material of component III, and add them to the main pot.

[0254] 4. Add 10% (w / w) arginine to the main pot, add deionized water to 100% (w / w), and adjust the pH to 6.

[0255] 5. Mix and stir evenly to obtain essence I containing the combination of Poria cocos extract and Astragalus membranaceus extract from Example 2.

[0256] Note: The percentages mentioned in this embodiment are mass ratios relative to the total mass of serum I.

[0257] II. Skin Red Area Percentage Test 1. Test Instruments and Methods 1.1 Test instrument: VISIA-CR5 (Canfield, USA).

[0258] 1.2 Test Methods: A VISIA-CR5 facial image analyzer (Canfield, USA) was used to acquire images of the test subjects' faces under cross-polarized light (CP) illumination. The percentage of red area was analyzed using Image ProPlus 7 software. Sixteen test subjects used the sample continuously for one week, once daily. A skin test (baseline value, Tbase) was performed before use, and another skin test (test value, T1wk) was performed after one week of continuous use. A significant reduction in the percentage of red area, and a smaller area of ​​skin redness, indicated that the sample had an effect on improving skin redness.

[0259] 1.3 Data Analysis: Significance Test: The Shapiro-Wilk Test was used to test the normality of the data variation values. If the distribution is normal, a one-sample T-test was performed with a significance level of α=0.05; if the distribution is non-normal, a Wilcoxon one-sample signed-rank test was performed with a significance level of α=0.05.

[0260] 2. Test results: such as Figure 9 As shown, compared with the baseline value, there was a significant improvement after one week of continuous use of the sample (p<0.01), indicating that the serum I containing the composition of Example 2 has the effect of improving skin redness.

[0261] III. Conclusion This embodiment prepares the composition of Example 2, which has skin antioxidant effects, into serum I, and conducts skin efficacy tests, such as... Figure 9 As shown, serum I containing the composition of Example 2 has a soothing effect.

[0262] Example 8: Skin care application of a combination of Poria cocos extract and Paris polyphylla extract I. Preparation of the essence containing the composition of Example 4 The combination of Poria cocos extract and Paris polyphylla extract from Example 4 was used to prepare skin care essence II. The preparation method was the same as in Example 7, except that 0.1% of the composition from Example 4 was used instead of 0.1% of the composition from Example 2 for component III.

[0263] II. Skin red zone a* value test 1. Test Instruments and Methods 1.1 Test instrument: VISIA-CR5 (Canfield, USA).

[0264] 1.2 Test Method: A VISIA-CR5 facial image analyzer (Canfield, USA) was used to acquire images of the test subjects' faces under cross-polarized light (CP) illumination. The red zone a* value was obtained through analysis using Image ProPlus 7 software. Sixteen test subjects used the sample continuously for one week, once daily. A skin test (baseline value, Tbase) was performed before use, and another skin test (test value, T1wk) was performed after one week of continuous use.

[0265] 1.3 Data Analysis: Same as the data analysis method in Example 7.

[0266] 2. Test results: such as Figure 10 As shown, compared with the baseline value, there was a significant improvement after one week of continuous use of the sample (p<0.05), indicating that the serum II containing the composition of Example 4 has the effect of improving skin redness.

[0267] III. Conclusion This embodiment prepares the composition of Example 4, which has skin antioxidant effects, into serum II, and conducts skin efficacy tests, such as... Figure 10 As shown, serum II containing the composition of Example 4 has a soothing effect.

[0268] Example 9: Skin care application of a combination of Astragalus membranaceus extract and Paris polyphylla extract I. Preparation of a face cream containing the composition of Example 6 1. Weigh 6% (w / w) butanediol, 2% (w / w) glycerol, 2% (w / w) glycerol polyether-26, 1% (w / w) inositol, 0.6% (w / w) 1,2-hexanediol, 0.4% (w / w) sodium polyacrylamide dimethyl taurate, 0.4% (w / w) raspberry ketone, 0.3% (w / w) hydrogenated lecithin, 0.05% (w / w) acrylate / C10-30 alkanol acrylate crosspolymer, and 0.03% (w / w) disodium EDTA. Add 10% (w / w) deionized water to obtain component IV. Add the raw materials to the main pot and mix thoroughly. 2. Weigh out 3% (w / w) isohexadecane, 3% (w / w) squalane, 2% (w / w) cetearyl alcohol oleate, 2% (w / w) sorbitan oleate, 2% (w / w) cetearyl alcohol, 1.5% (w / w) isononyl isononanoate, 1.5% (w / w) polydimethylsiloxane, 1% (w / w) camellia seed oil, 0.5% (w / w) shea butter, 0.3% (w / w) tocopheryl acetate and 0.02% (w / w) daily fragrance to obtain component V. Heat to 80℃, add to the main pot, mix and homogenize evenly, then cool to 37℃. 3. Weigh 0.1% (w / w) of the composition of Example 6 and 0.5% (w / w) of tetrahydromethylpyrimidine carboxylic acid, add 5% (w / w) of deionized water, mix and stir evenly to obtain component VI, and add it to the main pot; 4. Add 10% (w / w) arginine to the main pot, add deionized water to 100% (w / w), and adjust the pH to 6.5; 5. Mix and stir until homogeneous to obtain a face cream containing the composition of Example 6.

[0269] Note: The percentages mentioned in this embodiment are mass ratios relative to the total mass of the face cream.

[0270] II. Transdermal Water Loss (TEWL) Value Test 1. Test Instruments and Methods 1.1 Test instrument: Tewameter™ 300 (Courage+Khazaka, Germany), which continuously measures transepidermal water loss (TEWL) using an open cavity with a diameter of 10mm based on the Adolf Fick diffusion principle.

[0271] 1.2 Test Method: The instrument was used to continuously test the sample on the test subject's skin for 40 seconds, and the average value of the last 20 seconds was recorded. Sixteen test subjects used the sample continuously for one week, once daily. A skin test (baseline value, Tbase) was performed before use, and another skin test (test value, T1wk) was performed after one week of continuous use. A significant decrease in TEWL value indicates a better skin barrier, suggesting that the sample has an effect on improving the skin barrier. Transepidermal water loss (TEWL) value: Unit: g / h / m 2 .

[0272] 1.3 Data Analysis: Same as the data analysis method in Example 7.

[0273] 2. Test results: such as Figure 11 As shown, compared with the baseline value, the transepidermal water loss (TEWL) value was significantly improved after one week of continuous use of the sample (p<0.001), indicating that the face cream containing the composition of Example 6 has the effect of improving the skin barrier.

[0274] III. Conclusion This embodiment prepares the composition of Example 6, which has skin antioxidant effects, into a face cream and conducts skin efficacy tests, such as... Figure 11 As shown, the face cream containing the composition of Example 6 has the effect of repairing the skin barrier.

[0275] The above embodiments are only for further elaboration and explanation of the technical solutions of the present invention, so that those skilled in the art can more accurately understand the inventive concept and operation scheme of the present invention, and are not intended to further limit the present invention. Any non-prominent substantive features and non-significant improvements made by those skilled in the art on this basis shall fall within the protection scope of the present invention.

Claims

1. An antioxidant composition, characterized in that, The antioxidant composition comprises the following components in parts by weight: 1.25-5 parts of Paris polyphylla extract and 7.5-30 parts of Astragalus membranaceus extract.

2. The antioxidant composition according to claim 1, characterized in that, The antioxidant composition comprises the following components in parts by weight: 1.5 to 4.75 parts of Paris polyphylla extract and 7.5 to 20 parts of Astragalus membranaceus extract.

3. The antioxidant composition according to claim 1, characterized in that, The antioxidant composition comprises the following components in parts by weight: 1.75-4.5 parts of Paris polyphylla extract and 7.5-18 parts of Astragalus membranaceus extract.

4. The antioxidant composition according to claim 1, characterized in that, The antioxidant composition comprises the following components in parts by weight: 2-4.25 parts of Paris polyphylla extract and 8-18 parts of Astragalus membranaceus extract.

5. The antioxidant composition according to claim 1, characterized in that, The antioxidant composition comprises the following components in parts by weight: 2-4 parts of Paris polyphylla extract and 9-17 parts of Astragalus membranaceus extract; Preferably, the antioxidant composition comprises the following components in parts by weight: 2.25-3.5 parts of Paris polyphylla extract and 10-16 parts of Astragalus membranaceus extract; Preferably, the antioxidant composition comprises the following components in parts by weight: 2.5 parts of Paris polyphylla extract and 15 parts of Astragalus membranaceus extract.

6. The antioxidant composition according to claim 1, characterized in that, The Paris polyphylla extract is an alcoholic extract of Paris polyphylla; Preferably, the Paris polyphylla extract is an ethanol extract of Paris polyphylla; Preferably, the extraction method of the Paris polyphylla extract includes the following steps: (1) The crude powder of Paris polyphylla was soaked in an ethanol aqueous solution and then refluxed to extract the extract; (2) Filter the extract obtained in (1) and concentrate it to obtain a concentrated solution; (3) Load the concentrated solution obtained in (2) onto the adsorption resin, perform gradient elution, and collect the eluent; (4) Concentrate and dry the eluent obtained from (3) to obtain Paris polyphylla extract; Preferably, the ethanol-water solution in step (1) is 50%-90% v / v; Preferably, the ethanol-water solution in step (1) is 60%-85% v / v; Preferably, the ethanol-water solution in step (1) is 70%-80% v / v; Preferably, the soaking time is 10-120 min; Preferably, the soaking time is 15-90 min; Preferably, the soaking time is 20-60 minutes; Preferably, the reflux extraction is performed 1-10 times; Preferably, the reflux extraction is performed 2-8 times; Preferably, the reflux extraction is performed 3-6 times; Preferably, the reflux extraction time is 1-10 h / time; Preferably, the reflux extraction time is 2-8 hours per extraction. Preferably, the reflux extraction time is 2-5 hours per extraction. Preferably, the filter screen used for filtration is a 100-500 mesh screen; Preferably, the filter screen used for filtration is a 150-300 mesh screen; Preferably, the concentration in step (2) is selected from atmospheric pressure concentration or vacuum concentration; Preferably, the concentration in step (2) is selected from vacuum concentration; Preferably, the adsorption resin is selected from macroporous adsorption resins; Preferably, the sample loading flow rate is 0.1-6 BV / h; Preferably, the sample loading flow rate is 0.5-5 BV / h; Preferably, the sample loading flow rate is 0.8-4 BV / h; Preferably, the elution flow rate is 0.5-6 BV / h; Preferably, the elution flow rate is 1-5 BV / h; Preferably, the elution flow rate is 1.5-4 BV / h; Preferably, the gradient elution is performed by sequentially using pure water, 10%-35% v / v ethanol aqueous solution, 36%-55% v / v ethanol aqueous solution, 56%-70% v / v ethanol aqueous solution, 71%-85% v / v ethanol aqueous solution and 86%-99% v / v ethanol aqueous solution as eluents. Preferably, the gradient elution is performed by sequentially using pure water, 15%-35% v / v ethanol aqueous solution, 40%-55% v / v ethanol aqueous solution, 60%-70% v / v ethanol aqueous solution, 75%-85% v / v ethanol aqueous solution and 90%-99% v / v ethanol aqueous solution as eluents. Preferably, the gradient elution is performed by sequentially using pure water, 30% v / v ethanol aqueous solution, 50% v / v ethanol aqueous solution, 65% v / v ethanol aqueous solution, 80% v / v ethanol aqueous solution and 95% v / v ethanol aqueous solution as eluents; Preferably, in the gradient elution, the elution volume of different eluents is 1-5 BV; Preferably, in the gradient elution, the elution volume of different eluents is 1-4 BV; Preferably, in the gradient elution, the elution volume of different eluents is 2-3 BV; Preferably, the concentration in step (4) is selected from atmospheric pressure concentration or vacuum concentration; Preferably, the concentration in step (4) is selected from vacuum concentration; Preferably, the drying is vacuum drying; Preferably, the Paris polyphylla is selected from Paris polyphylla yunnanensis, Paris polyphylla sphericalis, Paris polyphylla var. chinensis, Paris polyphylla var. septemlobus, Paris polyphylla var. daliensis, Paris polyphylla var. polyphylla, Paris polyphylla var. septemlobus ... or Paris polyphylla var. septemlobus. Preferably, the Paris polyphylla is selected from Paris polyphylla var. spp.

7. The antioxidant composition according to claim 1, characterized in that, The Astragalus extract is an ammonia extract of Astragalus membranaceus; Preferably, the extraction method of the Astragalus extract includes the following steps: (1) The crude powder of Astragalus membranaceus was soaked in ammonia water and then refluxed to extract the extract; (2) Concentrate the extract obtained in (1) to obtain an extract; (3) Dry the extract obtained in (2) to obtain Astragalus extract; Preferably, the ammonia water in step (1) is 1~20 wt%; Preferably, the ammonia water in step (1) is 2~15 wt%; Preferably, the ammonia water in step (1) is 5~10 wt%; Preferably, the soaking time is 1-40 hours; Preferably, the soaking time is 5-35 hours; Preferably, the soaking time is 10-30 hours; Preferably, the reflux extraction is performed 1-10 times; Preferably, the reflux extraction is performed 2-8 times; Preferably, the reflux extraction is performed 3-6 times; Preferably, the reflux extraction time is 1-10 h / time; Preferably, the reflux extraction time is 2-8 hours per extraction. Preferably, the reflux extraction time is 2-5 hours per extraction. Preferably, the mass ratio of the extract to the paste in step (2) is 1:(0.5-2). Preferably, the mass ratio of the extract to the paste in step (2) is 1:(0.8-1.25). Preferably, the mass ratio of the extract to the paste in step (2) is 1:(0.9-1.1). Preferably, the Astragalus membranaceus is selected from Astragalus membranaceus or Astragalus mongholicus.

8. The use of the antioxidant composition according to any one of claims 1-7 in the preparation of skin care products; Preferably, the skincare product includes any one of skincare water, lotion, cream, mask, serum, essential oil, and spray; Preferably, the skincare product is an antioxidant skincare product; Preferably, the skin care product is a skin care product that repairs the skin barrier.

9. The use of the antioxidant composition according to any one of claims 1-7 in the preparation of antioxidants for skin care products; Preferably, the skincare product includes any one of skincare water, lotion, cream, mask, serum, essential oil, and spray; Preferably, the skincare product is an antioxidant skincare product; Preferably, the skin care product is a skin care product that repairs the skin barrier.

10. A skincare product, characterized in that, Includes the antioxidant composition according to any one of claims 1-7; Preferably, the skincare product comprises 0.05-1 wt% of Paris polyphylla extract and 0.1-1 wt% of Astragalus membranaceus extract; Preferably, the skincare product comprises 0.05-0.8 wt% Paris polyphylla extract and 0.2-0.8 wt% Astragalus membranaceus extract; Preferably, the skincare product comprises 0.1 wt% Paris polyphylla extract and 0.6 wt% Astragalus membranaceus extract; Preferably, the skincare product includes any one of skincare water, lotion, cream, mask, serum, essential oil, and spray; Preferably, the skincare product further includes excipients; Preferably, the excipients include one or more of polyols, emulsifiers, emollients, buffers, chelating agents, thickeners, pH adjusters, skin protectants, preservatives, or antioxidants; Preferably, the polyol comprises one or more of propylene glycol, glycerol, butylene glycol, pentanediol, hexanediol, diglycerol, caprylyl glycol, dipropylene glycol, sorbitol, polyethylene glycol-8, or glycerol polyether-26; Preferably, the emulsifier comprises one or more of hydrogenated lecithin, cetearyl oleate, sorbitan oleate, cetearyl alcohol, or bis-PEG-18 methyl ether dimethylsilane; Preferably, the emollient comprises one or more of squalane, isododecane, isohexadecane, isononyl isononanoate, polydimethylsiloxane, shea butter, caprylic / capric triglyceride, Camellia yunnanensis seed oil, or walnut oil. Preferably, the buffer comprises one or more of citric acid, sodium citrate, sodium phosphate, sodium hydrogen phosphate, disodium hydrogen phosphate, or barbiturate. Preferably, the chelating agent includes one or more of disodium EDTA, tetrasodium EDTA, or sodium hexametaphosphate; Preferably, the thickener comprises one or more of the following: methylcellulose, ethylcellulose, hydroxypropyl methylcellulose, sodium carboxymethylcellulose, sodium polyacrylate, carbomer, acrylate / C10-30 alkanol acrylate crosspolymer, xanthan gum, sclerotinia gum, or sodium polyacrylamide dimethyl taurate. Preferably, the pH adjuster includes one or more of citric acid, sodium citrate, arginine, sodium hydroxide, triethanolamine, or aminomethylpropanol; Preferably, the skin protectant comprises one or more of sodium hyaluronate, tetrahydromethylpyrimidine carboxylic acid, betaine, acetylglucosamine, β-glucan, acetylated sodium hyaluronate, inositol, or nicotinamide; Preferably, the preservative includes one or more of phenoxyethanol, methylisothiazolinone, benzoic acid, sodium benzoate, benzyl alcohol, chlorphenesin, benzyl alcohol, methylparaben, ethylparaben, or propylparaben. Preferably, the antioxidant includes one or more of p-hydroxyacetophenone, raspberry ketone, tocopheryl acetate, tocopherol, or ascorbic acid.

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