Bio-fermentation polypeptide ammonia-free hair dyeing cream and preparation method thereof

Abstract

This invention discloses a bio-fermented polypeptide ammonia-free hair dye, which is formulated by combining a traditional Chinese medicine fermentation liquid with an ammonia-free hair dye base. The traditional Chinese medicine fermentation liquid is obtained by water extraction and fermentation with compound probiotics from arborvitae leaves, processed Polygonum multiflorum, Angelica sinensis, dried ginger, Sophora flavescens, Kochia scoparia, mulberry leaves, Scutellaria baicalensis, Ligustrum lucidum, and Centella asiatica. The fermentation liquid is rich in polypeptides, amino acids, flavonoids, and polyphenols. This invention, by combining the fermentation liquid with a specific ammonia-free system, can protect sensitive scalps, preventing redness and itching, achieving zero irritation through transdermal absorption; reducing hair breakage and dryness, achieving hair care without damage; the entire system is gentle and controllable, resulting in even coloring without unevenness or staining; the product does not separate or settle over long-term storage, making it suitable for industrial production.

Description

Technical Field

[0001] This application relates to the field of daily chemical hair dyeing technology, specifically to a bio-fermented polypeptide ammonia-free hair dye and its preparation method. Background Technology

[0002] Currently, mainstream traditional chemical hair dyes rely on strong alkaline chemical additives such as ammonia and monoethanolamine for their core coloring principle. These alkaline agents forcefully open the hair cuticle structure, allowing pigment molecules to penetrate the hair shaft and achieve rapid coloring. While this process is mature, highly efficient, and suitable for most hair dyeing scenarios, it has several unavoidable technical drawbacks. These alkaline chemicals are extremely irritating, easily penetrating and irritating the scalp during the dyeing process, causing redness, itching, allergies, and other discomfort. Long-term use can damage the scalp barrier. Furthermore, forcibly opening and closing the hair cuticle damages the hair's cuticle and internal protein structure, leading to dry, frizzy, split, and dull hair. The products also have a pungent odor that can easily cause respiratory discomfort, resulting in a poor user experience and safety.

[0003] As consumer awareness of health continues to rise, consumers are demanding higher standards for the safety and gentleness of hair dyeing products. Ammonia-free hair dyeing has become a core development trend in the hair care and dyeing industry. However, current conventional ammonia-free hair dyeing products still have significant technological shortcomings, and industry pain points have not been completely resolved. While most ammonia-free formulas meet the gentleness standard, their coloring reaction rate is slow, resulting in poor coloring effects at low temperatures and in short periods, uneven dye adhesion, and insufficient color saturation and development in the final product. Furthermore, ammonia-free systems have weak pigment fixation capabilities, poor color-locking performance, and hair is prone to oxidation, fading, and yellowing after dyeing, leading to poor color longevity and significantly reducing product quality. It is difficult to balance gentleness and safety with high-quality hair dyeing results.

[0004] Traditional Chinese medicine fermentation technology has unique advantages in biomodification. It can degrade macromolecular substances of traditional Chinese medicine through microbial fermentation, and efficiently release a variety of natural active ingredients such as peptides, amino acids, flavonoids, and polyphenols. It has the effects of hair care and repair, anti-oxidation, and color fixing and locking, and is gentle and compatible with the scalp without irritation or damage.

[0005] Currently, there is no research or application in the industry of hair dyeing products that deeply integrate traditional Chinese medicine compound fermentation technology with an amino-free system. This invention innovatively integrates two major technology systems, precisely overcoming the dual shortcomings of traditional chemical hair dyeing and conventional amino-free hair dyeing. It combines multiple advantages such as safety and gentleness, rapid coloring, high color saturation, and long-lasting color retention, effectively filling the technological and product gap in this niche market and possessing extremely high industry promotion value and market application prospects. Summary of the Invention

[0006] To address the problems existing in the prior art, this invention provides a mild hair dye cream using a traditional Chinese medicine probiotic fermented polypeptide system and an ammonia-free formula, as well as its preparation method.

[0007] Specifically, the technical solution of the present invention is implemented as follows:

[0008] In a first aspect, the present invention provides a bio-fermented polypeptide ammonia-free hair dye, which is compounded from a traditional Chinese medicine fermentation liquid and an ammonia-free hair dye base; the traditional Chinese medicine fermentation liquid is obtained by water extraction of Platycladus orientalis leaves, processed Polygonum multiflorum, Angelica sinensis, dried ginger, Sophora flavescens, Kochia scoparia, mulberry leaves, Scutellaria baicalensis, Ligustrum lucidum, and Centella asiatica, followed by fermentation with compound probiotics; the traditional Chinese medicine fermentation liquid is rich in polypeptides, amino acids, flavonoids and polyphenols.

[0009] In one or more embodiments, the raw materials of the traditional Chinese medicine fermentation liquid, by weight, include: 8-12 parts of Platycladus orientalis leaves, 6-10 parts of processed Polygonum multiflorum, 6-12 parts of Angelica sinensis, 3-8 parts of dried ginger, 6-12 parts of Sophora flavescens, 5-10 parts of Kochia scoparia, 9-14 parts of mulberry leaves, 5-10 parts of Scutellaria baicalensis, 5-10 parts of Ligustrum lucidum, and 5-10 parts of Centella asiatica.

[0010] In one or more embodiments, the compound probiotic is at least two of Lactobacillus plantarum, Lactobacillus acidophilus, and Saccharomyces cerevisiae, and the inoculation amount of the compound probiotic is 2% to 5% of the total mass of the fermentation system.

[0011] In one or more embodiments, the compound probiotic is composed of Lactobacillus plantarum, Lactobacillus acidophilus and Saccharomyces cerevisiae in a mass ratio of 2:1:2.

[0012] In one or more embodiments, the hair dye cream comprises, by weight: 15-35 parts of traditional Chinese medicine fermentation liquid, 2-6 parts of stearyl alcohol, 1-5 parts of cetearyl alcohol, 1-4 parts of cetearyl alcohol polyoxyethylene ether-5, 0.5-3 parts of paraffin wax, 0.5-3 parts of oleic acid, 0.5-3 parts of lanolin alcohol, and BT-85. 0.5-3 parts, hair dye intermediate 5-15 parts, chelating agent 0.05-0.3 parts, paraffin 0.5-3 parts, monoethanolamine 1-5 parts, isopropanolamine 1-5 parts, propylene glycol 2-8 parts, hydrolyzed collagen 0.05-0.1 parts, hyaluronic acid 0.05-0.1 parts, adenosine 0.03-0.08 parts, arginine 0.01-0.05 parts, acetyltyrosine 0.01-0.05 parts, citrulline 0.01-0.05 parts, ornithine 0.01-0.05 parts, deionized water to make up to 100 parts.

[0013] In one or more embodiments, the hair dye cream comprises, by weight: 25 parts of traditional Chinese medicine fermentation liquid, 4 parts of stearyl alcohol, 3 parts of cetearyl alcohol, 2 parts of cetearyl alcohol polyoxyethylene ether-5, 2 parts of paraffin wax, 1.5 parts of oleic acid, 2 parts of lanolin alcohol, 2.5 parts of BT-85, 10 parts of hair dyeing intermediate, 0.1 parts of chelating agent, 1.5 parts of paraffin wax, 3 parts of monoethanolamine, 3 parts of isopropanolamine, 5 parts of propylene glycol, 0.08 parts of hydrolyzed collagen, 0.07 parts of hyaluronic acid, 0.05 parts of adenosine, 0.03 parts of arginine, 0.03 parts of acetyltyrosine, 0.02 parts of citrulline, 0.02 parts of ornithine, and deionized water to make up to 100 parts.

[0014] In one or more embodiments, the hair dyeing intermediate is selected from one or more of p-phenylenediamine, p-aminophenol, resorcinol, and p-phenylenediamine derivatives; the chelating agent is selected from one or more of disodium EDTA, tetrasodium EDTA, and trisodium HEDTA.

[0015] The shampoo described in this invention has a pH of 5.5-7.0, is weakly acidic, does not damage the scalp, has zero ammonia odor, zero irritation, is non-allergenic, and has obvious effects.

[0016] Secondly, the present invention provides a method for preparing the ammonia-free bio-fermented polypeptide hair dye according to any one of the above claims, comprising the following steps:

[0017] (1) Water extraction of Chinese herbal medicine: The Chinese herbal medicine is crushed, soaked in water, decocted and the filtrate is combined, concentrated and cooled to 35~40℃;

[0018] (2) Probiotic fermentation: Inoculate with compound probiotics for fermentation, then inactivate at 80℃ for 30 min, and filter to obtain traditional Chinese medicine fermentation liquid;

[0019] (3) Preparation of oil phase: Stearyl alcohol, cetearyl alcohol, cetearyl alcohol polyoxyethylene ether-5, paraffin, oleic acid, lanolin alcohol, and BT-85 are heated to 75~80℃ and stirred to dissolve;

[0020] (4) Aqueous phase preparation: Add propylene glycol, monoethanolamine, isopropanolamine, chelating agent, hydrolyzed collagen, hyaluronic acid, adenosine, arginine, acetylacetic acid, citrulline and ornithine to deionized water, heat to 75~80℃ and stir evenly.

[0021] (5) Emulsification system: The oil phase is slowly added to the water phase, homogenized and emulsified, and then cooled to 60°C;

[0022] (6) Add fermentation liquid: Add Chinese herbal fermentation liquid and hair dyeing intermediate, and stir to homogenize;

[0023] (7) pH adjustment and post-treatment: Adjust the pH to 5.5~7.0 with monoethanolamine, add paraffin, cool to below 40℃, defoam, filter, and fill to obtain the hair dye cream.

[0024] In one or more embodiments, in step S1, the pulverized Chinese herbal medicine is added to 10-15 times the amount of purified water, soaked for 1 hour, decocted twice for 1.5 hours each time, the filtrates are combined, and concentrated at 50°C to a relative density of 1.05-1.10.

[0025] In one or more embodiments, in step S2, the fermentation conditions are: anaerobic fermentation at 32~37℃ for 48~72h.

[0026] Compared with the prior art, the present invention has the following advantages:

[0027] This invention involves fermenting specific Chinese herbal medicine components. The resulting polypeptides embed into the hair cuticle, enhancing pigment adsorption and significantly improving colorfastness. The fermented small-molecule anti-inflammatory peptides reduce hair dyeing irritation. The fermented flavonoids and polyphenols delay fading, provide lasting shine, and combat hair aging. Among these, privet fruit releases active ingredients such as oleanolic acid and ursolic acid during fermentation, synergistically enhancing the hair-darkening and color-fixing effect, further improving pigment longevity. Centella asiatica releases active ingredients such as asiaticoside during fermentation, strengthening scalp soothing and barrier repair, further reducing hair dyeing irritation.

[0028] This invention, by combining the fermentation liquid with a specific ammonia-free system, can protect sensitive scalps, preventing redness and itching, and achieving zero irritation through the skin; it reduces hair breakage and dryness, achieving hair care without damaging the hair; the entire system is mild and controllable, resulting in hair dyeing without blemishes or spots, and achieving uniform coloring; the product does not separate or settle after long-term storage, making it suitable for industrial production.

[0029] To provide a clearer understanding of this application, the specific implementation methods of this application will be described below. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of this application clearer, the embodiments of this application will be described in further detail below.

[0031] It should be understood that the described embodiments are merely some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without creative effort are within the scope of protection of the embodiments of this application.

[0032] In the description of this application, it should be understood that the terms "first," "second," "third," etc., are used only to distinguish similar objects and are not necessarily used to describe a specific order or sequence, nor should they be construed as indicating or implying relative importance. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances. The singular forms "a," "the," and "the" used in this application and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise. The word "if" as used herein can be interpreted as "when," "when," or "in response to determination."

[0033] Furthermore, in the description of this application, unless otherwise stated, "multiple" means two or more. "And / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A alone, A and B simultaneously, or B alone. The character " / " generally indicates that the preceding and following related objects have an "or" relationship.

[0034] Example 1: A bio-fermented polypeptide ammonia-free hair dye A and its preparation method

[0035] A bio-fermented polypeptide ammonia-free hair dye A comprises, by weight: 25 parts of traditional Chinese medicine fermentation liquid, 4 parts of stearyl alcohol, 3 parts of cetearyl alcohol, 2 parts of cetearyl alcohol polyoxyethylene ether-5, 2 parts of paraffin wax, 1.5 parts of oleic acid, 2 parts of lanolin alcohol, 2.5 parts of BT-85, 10 parts of p-phenylenediamine, 0.1 parts of disodium EDTA, 1.5 parts of paraffin wax, 3 parts of monoethanolamine, 3 parts of isopropanolamine, 5 parts of propylene glycol, 0.08 parts of hydrolyzed collagen, 0.07 parts of hyaluronic acid, 0.05 parts of adenosine, 0.03 parts of arginine, 0.03 parts of acetyltyrosine, 0.02 parts of citrulline, 0.02 parts of ornithine, and deionized water to make up to 100 parts.

[0036] The raw materials of the traditional Chinese medicine fermentation liquid, by weight, include: 10 parts of Platycladus orientalis leaves, 8 parts of processed Polygonum multiflorum, 10 parts of Angelica sinensis, 6 parts of dried ginger, 8 parts of Sophora flavescens, 7 parts of Kochia scoparia, 11 parts of mulberry leaves, 9 parts of Scutellaria baicalensis, 8 parts of Ligustrum lucidum, and 7 parts of Centella asiatica. The raw materials are obtained by water extraction followed by fermentation with compound probiotics. The compound probiotics consist of Lactobacillus plantarum, Lactobacillus acidophilus, and Saccharomyces cerevisiae in a mass ratio of 2:1:2. The inoculation amount of the compound probiotics is 4% of the total mass of the fermentation system. The traditional Chinese medicine fermentation liquid is rich in polypeptides, amino acids, flavonoids, and polyphenols.

[0037] The preparation method of the bio-fermented polypeptide ammonia-free hair dye A includes the following steps:

[0038] (1) Water extraction of Chinese herbal medicine: Pulverize the Chinese herbal medicine, add 12 times the amount of purified water, soak for 1 hour, decoct twice, 1.5 hours each time, combine the filtrates, concentrate at 50°C to a relative density of 1.08, and cool to 38°C;

[0039] (2) Probiotic fermentation: Compound probiotics were introduced and anaerobic fermented at 35℃ for 60h, then inactivated at 80℃ for 30min, and the fermented liquid of traditional Chinese medicine was obtained after filtration.

[0040] (3) Preparation of oil phase: Stearyl alcohol, cetearyl alcohol, cetearyl alcohol polyoxyethylene ether-5, paraffin, oleic acid, lanolin alcohol, and BT-85 are heated to 78°C and stirred to dissolve.

[0041] (4) Aqueous phase preparation: Add propylene glycol, monoethanolamine, isopropanolamine, disodium EDTA, hydrolyzed collagen, hyaluronic acid, adenosine, arginine, acetyltyrosine, citrulline and ornithine to deionized water, heat to 76°C and stir until homogeneous.

[0042] (5) Emulsification system: The oil phase is slowly added to the water phase, homogenized and emulsified, and then cooled to 60°C;

[0043] (6) Add fermentation liquid: Add the fermentation liquid of traditional Chinese medicine and p-phenylenediamine, and stir to homogenize;

[0044] (7) pH adjustment and post-treatment: Adjust the pH to 6.0 with monoethanolamine, add paraffin, cool to below 40°C, defoam, filter, and fill to obtain the hair dye cream.

[0045] Example 2: A bio-fermented polypeptide ammonia-free hair dye B and its preparation method

[0046] A bio-fermented polypeptide ammonia-free hair dye B comprises, by weight: 15 parts of traditional Chinese medicine fermentation liquid, 2 parts of stearyl alcohol, 1 part of cetearyl alcohol, 1 part of cetearyl alcohol polyoxyethylene ether-5, 0.5 parts of paraffin, 3 parts of oleic acid, 3 parts of lanolin alcohol, 0.5 parts of BT-85, 5 parts of p-aminophenol, 0.05 parts of tetrasodium EDTA, 0.5 parts of paraffin, 1 part of monoethanolamine, 1 part of isopropanolamine, 2 parts of propylene glycol, 0.05 parts of hydrolyzed collagen, 0.05 parts of hyaluronic acid, 0.03 parts of adenosine, 0.01 parts of arginine, 0.01 parts of acetyltyrosine, 0.01 parts of citrulline, 0.01 parts of ornithine, and deionized water to make up to 100 parts.

[0047] The raw materials of the traditional Chinese medicine fermentation liquid, by weight, include: 12 parts of Platycladus orientalis leaves, 10 parts of processed Polygonum multiflorum, 12 parts of Angelica sinensis, 8 parts of dried ginger, 12 parts of Sophora flavescens, 10 parts of Kochia scoparia, 14 parts of mulberry leaves, 10 parts of Scutellaria baicalensis, 10 parts of Ligustrum lucidum, and 10 parts of Centella asiatica. The raw materials are obtained by water extraction followed by fermentation with compound probiotics. The compound probiotics consist of Lactobacillus plantarum and Lactobacillus acidophilus in a mass ratio of 2:1. The inoculum amount of the compound probiotics is 2% of the total mass of the fermentation system. The traditional Chinese medicine fermentation liquid is rich in polypeptides, amino acids, flavonoids, and polyphenols.

[0048] The preparation method of the bio-fermented polypeptide ammonia-free hair dye B includes the following steps:

[0049] (1) Water extraction of Chinese herbal medicine: Pulverize the Chinese herbal medicine, add 10 times the amount of purified water, soak for 1 hour, decoct twice, 1.5 hours each time, combine the filtrates, concentrate at 50°C to a relative density of 1.05, and cool to 35°C;

[0050] (2) Probiotic fermentation: Compound probiotics were introduced and anaerobic fermented at 32℃ for 72h, then inactivated at 80℃ for 30min, and the fermented liquid of traditional Chinese medicine was obtained after filtration.

[0051] (3) Preparation of oil phase: Stearyl alcohol, cetearyl alcohol, cetearyl alcohol polyoxyethylene ether-5, paraffin, oleic acid, lanolin alcohol, and BT-85 are heated to 75°C and stirred to dissolve.

[0052] (4) Aqueous phase preparation: Add propylene glycol, monoethanolamine, isopropanolamine, tetrasodium EDTA, hydrolyzed collagen, hyaluronic acid, adenosine, arginine, acetyltyrosine, citrulline and ornithine to deionized water, heat to 75°C and stir until homogeneous.

[0053] (5) Emulsification system: The oil phase is slowly added to the water phase, homogenized and emulsified, and then cooled to 60°C;

[0054] (6) Add fermentation broth: Add the fermentation broth of traditional Chinese medicine and p-aminophenol, and stir to homogenize;

[0055] (7) pH adjustment and post-treatment: Adjust the pH to 5.5 with monoethanolamine, add paraffin, cool to below 40°C, defoam, filter, and fill to obtain the hair dye cream.

[0056] Example 3: A bio-fermented polypeptide ammonia-free hair dye C and its preparation method

[0057] A bio-fermented polypeptide ammonia-free hair dye C comprises, by weight: 35 parts of traditional Chinese medicine fermentation liquid, 6 parts of stearyl alcohol, 5 parts of cetearyl alcohol, 4 parts of cetearyl alcohol polyoxyethylene ether-5, 3 parts of paraffin wax, 0.5 parts of oleic acid, 0.5 parts of lanolin alcohol, 3 parts of BT-85, 15 parts of resorcinol, 0.3 parts of trisodium HEDTA, 3 parts of paraffin wax, 5 parts of monoethanolamine, 5 parts of isopropanolamine, 8 parts of propylene glycol, 0.1 parts of hydrolyzed collagen, 0.1 parts of hyaluronic acid, 0.08 parts of adenosine, 0.05 parts of arginine, 0.05 parts of acetyltyrosine, 0.05 parts of citrulline, 0.05 parts of ornithine, and deionized water to make up to 100 parts.

[0058] The raw materials of the traditional Chinese medicine fermentation liquid, by weight, include: 12 parts of Platycladus orientalis leaves, 6 parts of processed Polygonum multiflorum, 6 parts of Angelica sinensis, 3 parts of dried ginger, 6 parts of Sophora flavescens, 5 parts of Kochia scoparia, 9 parts of mulberry leaves, 5 parts of Scutellaria baicalensis, 5 parts of Ligustrum lucidum, and 5 parts of Centella asiatica. The raw materials are obtained by water extraction followed by fermentation with compound probiotics. The compound probiotics consist of Lactobacillus acidophilus and Saccharomyces cerevisiae in a 1:1 mass ratio. The inoculum amount of the compound probiotics is 5% of the total mass of the fermentation system. The traditional Chinese medicine fermentation liquid is rich in polypeptides, amino acids, flavonoids, and polyphenols.

[0059] The preparation method of the above-described bio-fermented polypeptide ammonia-free hair dye C includes the following steps:

[0060] (1) Water extraction of Chinese herbal medicine: Pulverize the Chinese herbal medicine, add 15 times the amount of purified water, soak for 1 hour, decoct twice, 1.5 hours each time, combine the filtrates, concentrate at 50°C to a relative density of 1.10, and cool to 40°C;

[0061] (2) Probiotic fermentation: Compound probiotics were introduced and anaerobic fermented at 37°C for 48 hours, then inactivated at 80°C for 30 minutes, and the fermented liquid of traditional Chinese medicine was obtained after filtration.

[0062] (3) Preparation of oil phase: Stearyl alcohol, cetearyl alcohol, cetearyl alcohol polyoxyethylene ether-5, paraffin, oleic acid, lanolin alcohol, and BT-85 are heated to 80°C and stirred to dissolve.

[0063] (4) Aqueous phase preparation: Add propylene glycol, monoethanolamine, isopropanolamine, trisodium HEDTA, hydrolyzed collagen, hyaluronic acid, adenosine, arginine, acetyltyrosine, citrulline and ornithine to deionized water, heat to 80°C and stir until homogeneous.

[0064] (5) Emulsification system: The oil phase is slowly added to the water phase, homogenized and emulsified, and then cooled to 60°C;

[0065] (6) Add fermentation liquid: Add the fermentation liquid of traditional Chinese medicine and resorcinol, and stir to homogenize;

[0066] (7) pH adjustment and post-treatment: Adjust the pH to 7.0 with monoethanolamine, add paraffin, cool to below 40°C, defoam, filter, and fill to obtain the hair dye cream.

[0067] Comparative Example 1: An ammonia-free hair dye D and its preparation method

[0068] A non-ammonia hair dye D is basically the same as the bio-fermented polypeptide non-ammonia hair dye A described in Example 1, except that the Chinese herbal medicine raw material is not fermented. The rest of the preparation method is the same as in Example 1.

[0069] Test Example 1: Stability Test

[0070] The stability of the hair dyes in Examples 1-3 and Comparative Example 1 was tested over a period of 90 days. After the test period ended, the samples were removed and allowed to stand at room temperature for 24 hours to allow them to return to normal temperature. Subsequently, visual observation and property evaluation were carried out item by item.

[0071] The results are as follows:

[0072] Products from Examples 1-3: The paste is uniform and delicate, without layering, water separation, or floating oil. The color of the paste does not change significantly, and no rancid odor is produced. The system is stable.

[0073] The product of Comparative Example 1 showed slight layering / yellowing / lightening of color after storage, and its system stability was inferior to that of the products of Examples 1-3.

[0074] The results above show that the bio-fermented polypeptide ammonia-free hair dye of the present invention has good stability, is suitable for large-scale industrial production, and is easy to promote and apply.

[0075] Test Example 2: Test on the effectiveness of hair dye

[0076] This test example measured the effectiveness of the hair dye in Examples 1-3 and Comparative Example 1.

[0077] Forty volunteers aged 30-50 were selected and randomly divided into four groups of 10 each. Each group of volunteers underwent a clinical test using a different hair dye. The volunteers were evaluated on three indicators: the vibrancy of the hair color (divided into three categories: very vibrant, relatively vibrant, and normal), the evenness of the color (divided into three categories: very even, relatively even, and normal), and the scalp irritation.

[0078] Evaluation scores: The corresponding scores for the vividness of hair dyeing are 1 to 5 points, with higher scores for more vivid dyeing; the corresponding scores for the evenness of dyeing are 1 to 5 points, with higher scores for more even dyeing; the corresponding scores for scalp irritation are 1 to 5 points, with higher scores for less irritation, and 5 points for no irritation.

[0079] The effects of use are shown in Table 1:

[0080] Table 1. Effects of using hair dye

[0081] Group Hair dye vibrancy Dyeing uniformity scalp irritation Example 1 5 5 5 Example 2 4 5 5 Example 3 5 4 5 Comparative Example 1 2 3 1

[0082] The results show that the hair dye cream of the present invention, by combining fermentation liquid with a specific ammonia-free system, can achieve zero irritation through the skin, and the whole system is mild and controllable, resulting in hair dyeing without blemishes or spots, and with even coloring.

[0083] It should also be noted that the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.

[0084] The above are merely embodiments of this application and are not intended to limit the scope of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.

Claims

1. A bio-fermented polypeptide ammonia-free hair dye, characterized in that, It is a compound of traditional Chinese medicine fermentation liquid and ammonia-free hair dye base; the traditional Chinese medicine fermentation liquid is made by extracting the following herbs by water and then fermenting them with compound probiotics: Platycladus orientalis leaves, processed Polygonum multiflorum, Angelica sinensis, dried ginger, Sophora flavescens, Kochia scoparia, mulberry leaves, Scutellaria baicalensis, Ligustrum lucidum, and Centella asiatica; the traditional Chinese medicine fermentation liquid is rich in polypeptides, amino acids, flavonoids and polyphenols.

2. The bio-fermented polypeptide ammonia-free hair dye according to claim 1, characterized in that, The raw materials of the fermented Chinese medicine liquid, by weight, include: 8-12 parts of arborvitae leaves, 6-10 parts of processed Polygonum multiflorum, 6-12 parts of Angelica sinensis, 3-8 parts of dried ginger, 6-12 parts of Sophora flavescens, 5-10 parts of Kochia scoparia, 9-14 parts of mulberry leaves, 5-10 parts of Scutellaria baicalensis, 5-10 parts of Ligustrum lucidum, and 5-10 parts of Centella asiatica.

3. The bio-fermented polypeptide ammonia-free hair dye according to claim 1, characterized in that, The compound probiotics are at least two of Lactobacillus plantarum, Lactobacillus acidophilus, and Saccharomyces cerevisiae, and the inoculation amount of the compound probiotics is 2% to 5% of the total mass of the fermentation system.

4. The bio-fermented polypeptide ammonia-free hair dye according to claim 1, characterized in that, The compound probiotics consist of Lactobacillus plantarum, Lactobacillus acidophilus, and Saccharomyces cerevisiae in a mass ratio of 2:1:

2.

5. The bio-fermented polypeptide ammonia-free hair dye according to claim 1, characterized in that, The hair dye cream comprises, by weight, 15-35 parts of traditional Chinese medicine fermentation liquid, 2-6 parts of stearyl alcohol, 1-5 parts of cetearyl alcohol, 1-4 parts of cetearyl alcohol polyoxyethylene ether-5, 0.5-3 parts of paraffin wax, 0.5-3 parts of oleic acid, 0.5-3 parts of lanolin alcohol, and BT-85. 0.5-3 parts, hair dye intermediate 5-15 parts, chelating agent 0.05-0.3 parts, paraffin 0.5-3 parts, monoethanolamine 1-5 parts, isopropanolamine 1-5 parts, propylene glycol 2-8 parts, hydrolyzed collagen 0.05-0.1 parts, hyaluronic acid 0.05-0.1 parts, adenosine 0.03-0.08 parts, arginine 0.01-0.05 parts, acetyltyrosine 0.01-0.05 parts, citrulline 0.01-0.05 parts, ornithine 0.01-0.05 parts, deionized water to make up to 100 parts.

6. The bio-fermented polypeptide ammonia-free hair dye according to claim 5, characterized in that, The hair dye cream comprises, by weight, 25 parts of traditional Chinese medicine fermentation liquid, 4 parts of stearyl alcohol, 3 parts of cetearyl alcohol, 2 parts of cetearyl alcohol polyoxyethylene ether-5, 2 parts of paraffin wax, 1.5 parts of oleic acid, 2 parts of lanolin alcohol, 2.5 parts of BT-85, 10 parts of hair dyeing intermediate, 0.1 parts of chelating agent, 1.5 parts of paraffin wax, 3 parts of monoethanolamine, 3 parts of isopropanolamine, 5 parts of propylene glycol, 0.08 parts of hydrolyzed collagen, 0.07 parts of hyaluronic acid, 0.05 parts of adenosine, 0.03 parts of arginine, 0.03 parts of acetyltyrosine, 0.02 parts of citrulline, 0.02 parts of ornithine, and deionized water to make up to 100 parts.

7. The bio-fermented polypeptide ammonia-free hair dye according to claim 5, characterized in that, The hair dyeing intermediate is selected from one or more of p-phenylenediamine, p-aminophenol, resorcinol, and p-phenylenediamine derivatives; the chelating agent is selected from one or more of disodium EDTA, tetrasodium EDTA, and trisodium HEDTA.

8. The method for preparing the ammonia-free bio-fermented polypeptide hair dye according to any one of claims 1 to 7, characterized in that, Includes the following steps: (1) Water extraction of Chinese herbal medicine: The Chinese herbal medicine is crushed, soaked in water, decocted and the filtrate is combined, concentrated and cooled to 35~40℃; (2) Probiotic fermentation: Inoculate with compound probiotics for fermentation, then inactivate at 80℃ for 30 min, and filter to obtain traditional Chinese medicine fermentation liquid; (3) Preparation of oil phase: Stearyl alcohol, cetearyl alcohol, cetearyl alcohol polyoxyethylene ether-5, paraffin, oleic acid, lanolin alcohol, and BT-85 are heated to 75~80℃ and stirred to dissolve; (4) Aqueous phase preparation: Add propylene glycol, monoethanolamine, isopropanolamine, chelating agent, hydrolyzed collagen, hyaluronic acid, adenosine, arginine, acetylacetic acid, citrulline and ornithine to deionized water, heat to 75~80℃ and stir evenly. (5) Emulsification system: The oil phase is slowly added to the water phase, homogenized and emulsified, and then cooled to 60°C; (6) Add fermentation liquid: Add Chinese herbal fermentation liquid and hair dyeing intermediate, and stir to homogenize; (7) pH adjustment and post-treatment: Adjust the pH to 5.5~7.0 with monoethanolamine, add paraffin, cool to below 40℃, defoam, filter, and fill to obtain the hair dye cream.

9. The preparation method according to claim 8, characterized in that, In step S1, the pulverized Chinese herbal medicine is added to 10-15 times the amount of purified water, soaked for 1 hour, decocted twice for 1.5 hours each time, the filtrates are combined, and concentrated at 50°C to a relative density of 1.05-1.

10.

10. The preparation method according to claim 8, characterized in that, In step S2, the fermentation conditions are: anaerobic fermentation at 32~37℃ for 48~72h.