A traditional Chinese medicine composition for treating necrotic enteritis of chicken, a preparation method and application thereof

Abstract

The application discloses a traditional Chinese medicine composition for treating chicken necrotic enteritis and a preparation method and application thereof. The traditional Chinese medicine composition comprises the following components in parts by weight: Andrographis paniculata 70-80 parts, Fructus Mume 60-65 parts, Magnolia officinalis 55-60 parts, Areca nut 40-50 parts, Agrimonia pilosa 40-45 parts and Glycyrrhiza 10-15 parts, and has the effects of clearing heat and resolving toxins, cooling blood and stopping diarrhea, and promoting qi and killing insects. Test results show that the application has a good treatment effect on chicken necrotic enteritis, can improve the growth performance, immune function and antioxidant performance of meat chickens infected with necrotic enteritis, and can repair the intestinal barrier to a certain extent, and the treatment effect of a high-dose Chuanwu Houhu He Decoction is better. The application can replace antibiotics to treat chicken necrotic enteritis, can reduce drug residues, guarantee food safety, can promote poultry breeding to develop in a green direction, and realizes a win-win of economic and social benefits.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese veterinary medicine technology, and in particular to a traditional Chinese medicine composition for treating necrotizing enteritis in chickens, its preparation method, and its application. Background Technology

[0002] Necrotic enteritis in chickens is an intestinal disease caused by the overgrowth of Clostridium perfringens (types A, C, or G). Clostridium perfringens is normally a component of the normal flora in the animal gut; however, when chickens are infected with coccidia, fed high-concentration animal protein feed, or have excessively high stocking densities, the intestinal microecology becomes imbalanced, leading to the overgrowth of this bacterium and resulting in necrotic enteritis. Affected chickens exhibit symptoms such as lethargy, ruffled and disheveled feathers, dehydration, and bloody diarrhea. Their production performance declines, and the mortality rate can reach up to 50%, posing a significant threat. Both broilers and laying hens can be infected and develop the disease, with broilers aged 2-5 weeks and laying hens older than 5 weeks being particularly susceptible.

[0003] For a long time, antibiotics have dominated the prevention and treatment of necrotizing enteritis in chickens. Antibiotics can inhibit the growth of pathogens to a certain extent and quickly alleviate symptoms in chickens. However, the long-term and excessive use of antibiotics has brought many problems. On the one hand, the overuse of antibiotics has led to the emergence of drug-resistant strains, rendering previously effective antibiotics less effective and increasing the difficulty of disease control. On the other hand, antibiotic residues in chicken meat and eggs seriously threaten food safety and pose a potential risk to human health. Meanwhile, with the increasing demand from consumers for green and healthy poultry products, reducing antibiotic use has become an inevitable trend in the poultry industry.

[0004] Against this backdrop, traditional Chinese medicine (TCM) formulas have gradually gained attention due to their unique advantages. TCM formulas are composed of various medicinal herbs according to specific compatibility principles, resulting in complex and diverse components and more multifaceted mechanisms of action. They not only possess antibacterial and anti-inflammatory effects, inhibiting the growth and reproduction of pathogens such as Clostridium perfringens, but also regulate the immune function of chickens, enhancing their own resistance and fundamentally resisting disease invasion. Moreover, most TCM herbs are derived from natural plants, animals, and minerals, metabolized quickly in the body, leaving virtually no residue and posing no threat to food safety. Furthermore, TCM formulas have advantages such as fewer side effects and a lower likelihood of inducing drug resistance, aligning with the green, environmentally friendly, and healthy development concepts of modern poultry farming. Therefore, in-depth research and development of TCM formulas for treating necrotizing enteritis in chickens is of great significance for overcoming the current antibiotic dilemma facing the poultry industry and achieving green, healthy, and sustainable development in poultry farming. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a traditional Chinese medicine composition with reasonable formulation and good effect for treating necrotizing enteritis in broilers, as well as its preparation method and application.

[0006] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0007] A traditional Chinese medicine composition, by mass parts, includes the following raw materials: 70-80 parts of Andrographis paniculata, 60-65 parts of Prunus mume, 55-60 parts of Magnolia officinalis, 40-50 parts of Areca catechu, 40-45 parts of Agrimonia pilosa, and 10-15 parts of Glycyrrhiza uralensis.

[0008] Furthermore, the aforementioned traditional Chinese medicine composition, by mass parts, includes the following raw materials: 75 parts of Andrographis paniculata, 60 parts of Prunus mume, 60 parts of Magnolia officinalis, 45 parts of Areca catechu, 45 parts of Agrimonia pilosa, and 15 parts of Glycyrrhiza uralensis.

[0009] The present invention also provides a method for preparing the above-mentioned traditional Chinese medicine composition, comprising the following steps:

[0010] Add 10 times its weight of distilled water to the raw materials and soak for 12 hours; boil and simmer for 1 hour, then filter the liquid; add 8 times its weight of distilled water to the dregs, boil and simmer for 1 hour, then filter the liquid; repeat the same steps for the third decoction. Combine the liquids obtained from the three filtrations and concentrate them using a rotary evaporator to a final concentration of 1 g / mL to obtain the traditional Chinese medicine composition (Chuanwu Houhe Decoction).

[0011] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition in the preparation of a drug for treating necrotizing enteritis in chickens.

[0012] Furthermore, the application includes its use in the preparation of drugs to improve the growth performance of broilers infected with necrotizing enteritis.

[0013] Furthermore, the application includes its use in the preparation of drugs that enhance the immune function of broilers infected with necrotizing enteritis.

[0014] Furthermore, the application includes its use in the preparation of drugs that enhance the antioxidant properties of broilers infected with necrotizing enteritis.

[0015] Furthermore, the application includes its use in the preparation of drugs for repairing the intestinal barrier in broilers infected with necrotizing enterocolitis.

[0016] Furthermore, the necrotizing enteritis in chickens is caused by a mixed infection of Clostridium perfringens type A and Eimeria giantiformis.

[0017] Furthermore, the drug for treating necrotizing enteritis in chickens is administered orally at a dosage of 5-15 mL / L water.

[0018] This invention has the effects of clearing heat and detoxifying, cooling blood and stopping dysentery, promoting qi circulation and killing parasites. Andrographis paniculata is the principal herb, which is bitter and cold in nature, entering the heart, lung, large intestine, and bladder meridians, and has the effects of clearing heat and detoxifying, cooling blood and reducing swelling; Prunus mume is sour and astringent in nature, entering the liver, spleen, lung, and large intestine meridians, and has the effects of astringing the intestines and stopping diarrhea, and astringing the lungs and promoting fluid production; Magnolia officinalis is bitter, pungent, and warm in nature, entering the spleen, stomach, lung, and large intestine meridians, and has the effects of lowering qi and relieving fullness; these two are the assistant herbs; Areca catechu is bitter, pungent, and warm in nature, entering the stomach and large intestine meridians, and has the effects of killing parasites, eliminating stagnation, lowering qi and promoting water circulation; Agrimonia pilosa is bitter, astringent, and neutral in nature, entering the heart and liver meridians, and has the effects of astringing and stopping bleeding; these two are the adjuvant herbs; Glycyrrhiza uralensis is sweet and neutral in nature, entering the heart, lung, spleen, and stomach meridians, and has the effects of tonifying the spleen and replenishing qi, and harmonizing the effects of the other herbs; it is the guiding herb.

[0019] Compared with the prior art, the present invention has the following technical advantages:

[0020] (1) The present invention has a good therapeutic effect on necrotizing enteritis in chickens, and can improve the growth performance, immune function and antioxidant performance of broilers infected with necrotizing enteritis, and can repair the intestinal barrier of broilers infected with necrotizing enteritis.

[0021] (2) All the medicinal materials in this invention are commonly used traditional medicinal materials. It is a drug that is effective, pollution-free, residue-free and easy to use for treating necrotizing enteritis in chickens. This invention provides a brand-new non-antibiotic solution for the prevention and treatment of necrotizing enteritis, and is expected to replace traditional antibiotics. It provides a new technical path for solving the problem of antibiotic dependence in the current prevention and treatment of necrotizing enteritis. Attached Figure Description

[0022] Figure 1 To show the growth performance of each group, in the figure: (A) is the average daily weight gain of each group, (B) is the average daily feed intake of each group, and (C) is the feed conversion ratio of each group; where NC is the blank control group, NE is the model group, BMD is the succinate group, LC is the low-dose Chuanwu Houhe decoction group, MC is the medium-dose Chuanwu Houhe decoction group, and HC is the high-dose Chuanwu Houhe decoction group.

[0023] Figure 2 The intestinal necropsy results and intestinal lesion scores for each group are shown in the figure. (A) shows the jejunum necropsy results, (B) shows the ileum necropsy results, (C) shows the jejunum lesion score, and (D) shows the ileum lesion score. Among them, NC is the blank control group, NE is the model group, BMD is the succinate group, LC is the low-dose Chuanwu Houhe Decoction group, MC is the medium-dose Chuanwu Houhe Decoction group, and HC is the high-dose Chuanwu Houhe Decoction group.

[0024] Figure 3 The images show jejunal pathological sections. In the figures: (A) is the blank control group, (B) is the model group, (C) is the succinate group, (D) is the low-dose Chuanwu Houhe Decoction group, (E) is the medium-dose Chuanwu Houhe Decoction group, and (F) is the high-dose Chuanwu Houhe Decoction group.

[0025] Figure 4 The figure shows the concentration of FITC in serum. In the figure, NC is the blank control group, NE is the model group, BMD is the succinate group, LC is the low-dose Chuanwu Houhe Decoction group, MC is the medium-dose Chuanwu Houhe Decoction group, and HC is the high-dose Chuanwu Houhe Decoction group.

[0026] Figure 5 The figures show the relative expression levels of genes related to the jejunal barrier. In the figure: (A) is the relative expression level of the Claudin-1 gene; (B) is the relative expression level of the Occludin gene; (C) is the relative expression level of the Mucin-2 gene; and (D) is the relative expression level of the ZO-1 gene. Among them, NC is the blank control group, NE is the model group, BMD is the succinate group, LC is the low-dose Chuanwu Houhe Decoction group, MC is the medium-dose Chuanwu Houhe Decoction group, and HC is the high-dose Chuanwu Houhe Decoction group.

[0027] Figure 6 The values ​​represent serum antioxidant indicators. In the figure: (A) represents total antioxidant capacity (T-AOC); (B) represents peroxidase (CAT); (C) represents superoxide dismutase (SOD); (D) represents malondialdehyde (MDA); where NC represents the blank control group, NE represents the model group, BMD represents the succinate group, LC represents the low-dose Chuanwu Houhe Decoction group, MC represents the medium-dose Chuanwu Houhe Decoction group, and HC represents the high-dose Chuanwu Houhe Decoction group.

[0028] Figure 7 The figures show the concentrations of various cytokines: (A) serum IgA concentration; (B) serum IgG concentration; (C) serum IgM concentration; (D) IL-1 concentration in jejunal tissue; (E) IL-6 concentration in jejunal tissue; (F) IL-10 concentration in jejunal tissue; (G) TNF-α concentration in jejunal tissue. NC represents the blank control group, NE represents the model group, BMD represents the succinate group, LC represents the low-dose Chuanwu Houhe Decoction group, MC represents the medium-dose Chuanwu Houhe Decoction group, and HC represents the high-dose Chuanwu Houhe Decoction group. Detailed Implementation

[0029] 1 Method

[0030] 1.1 Preparation of Traditional Chinese Medicine Compositions

[0031] Andrographis paniculata, dried plum, magnolia bark, areca nut, agrimony, and licorice were purchased from Hunan Songlingtang Traditional Chinese Medicine Pieces Co., Ltd. 75g of Andrographis paniculata, 60g of dried plum, 60g of magnolia bark, 45g of areca nut, 45g of agrimony, and 15g of licorice were weighed separately, mixed, and then 1200mL of distilled water was added. The mixture was soaked for 12 hours and then decocted. After boiling, the decoction was simmered for 1 hour and filtered to obtain the liquid. Then, 960mL of distilled water was added to the dregs for a second decoction. After boiling, the decoction was simmered for 1 hour and filtered to obtain the liquid. The third decoction was performed using the same steps as the second. The three filtrations were combined, and the liquid was concentrated using a rotary evaporator to a final concentration of 1g / mL. The resulting Andrographis paniculata-dried plum, magnolia bark, areca nut, agrimony, and licorice decoction was stored at 4℃ for later use.

[0032] 1.2 Preparation of giant Eimeria coccidia oocysts

[0033] Eimeria giantiformis oocysts were obtained from the Traditional Chinese Veterinary Laboratory of the College of Animal Science and Technology, Guangxi University. Sporulated oocysts of Eimeria giantiformis stored at 4℃ were passaged in 14-day-old coccidia-free broiler chickens. Purification, sporulation, and oocyst counting of Eimeria giantiformis were performed according to standard methods. To induce necrotizing enteritis caused by Clostridium perfringens, purified Eimeria giantiformis were stored at 4℃ for no more than one month to ensure oocyst viability.

[0034] 1.3 Preparation of Clostridium perfringens strains

[0035] Clostridium perfringens type A CVCC2030 was purchased from the China Veterinary Microbial Culture Collection Center. CVCC2030 was removed from cryopreservation tubes and inoculated onto meat broth. After overnight incubation at 37°C, the culture was streaked onto TSC medium and anaerobically cultured for 24 hours. Black single colonies were picked and re-inoculated onto meat broth, cultured to the logarithmic growth phase, and stored for later use. The reserved bacterial culture was diluted 1:1000 into fresh meat broth and anaerobically cultured at 37°C for 11 hours. The resulting fresh culture was centrifuged at 4000 rpm for 10 minutes at 4°C to collect the cells. The cells were washed three times with sterile PBS, and the bacterial concentration was adjusted with PBS. The bacterial concentration was determined to be 1 × 10⁻⁶ using the plate count method. 9 CFU / mL.

[0036] 1.4 Experimental grouping and treatment

[0037] One hundred and forty-four one-day-old male broiler chickens were acclimatized for 13 days. On day 14, the chickens were randomly divided into six groups: blank control group (NC group), model group (NE group), bromide group (BMD group), low-dose Chuanwu Houhe decoction group (LC group), medium-dose Chuanwu Houhe decoction group (MC group), and high-dose Chuanwu Houhe decoction group (HC group). Each group had three replicates, with eight chickens per replicate. The NC group was administered 1 mL of sterile PBS by gavage, while the other groups were administered PBS at a concentration of 1 × 10⁻⁶. 5 1 mL of a solution of sporulated oocysts of *Eimeria tenella* per mL was administered. From day 17 to 23, the NC group was orally administered 1 mL of sterile PBS daily, while the other groups were orally administered a solution of 1 × 10⁻⁶ PBS daily. 9 1 mL of Clostridium perfringens bacterial suspension with CFU / mL was added. From day 24 to day 28, the BMD group was treated with 0.2 g of Clostridium perfringens added per liter of drinking water, while the LC, MC, and HC groups were treated with 5 mL, 10 mL, and 15 mL of Clostridium perfringens decoction added per liter of drinking water, respectively. The other groups drank water normally. The formal trial period was 15 days.

[0038] 1.5 Feeding and Management

[0039] All broilers were housed in well-ventilated rooms, with 23 hours of light and 1 hour of darkness per day. The room temperature was maintained at 33°C for the first week, then decreased by 1°C daily until reaching 26°C. From the start to the end of the experiment, the broilers had free access to food and water. They were also vaccinated according to the standard immunization schedule. The composition and nutrient levels of the basal diet are shown in Table 1.

[0040] Table 1. Composition and nutrient levels of basal diet (air-dried basal diet)

[0041]

[0042] Note: 1. The premix provides the following per kilogram of feed: VA 10000 IU, VD 5200 IU, VE 40 IU, VK 8 IU, VB 15 IU, VB 28 IU, VB 612 IU, VB 12 2 IU, Biotin 0.2 mg, Niacin 45 mg, Folic Acid 0.8 mg, Iron 100 mg, Copper 8 mg, Zinc 120 mg, Manganese 120 mg, Iodine 0.3 mg, Selenium 0.7 mg.

[0043] 1.6 Growth performance

[0044] On days 14 and 29, after fasting the chicks for 12 hours while ensuring normal water supply, the chicks in each replicate group were weighed on an empty stomach. Feed intake throughout the entire experimental period was recorded to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (F / G).

[0045] 1.7 Intestinal lesion score

[0046] On day 29, three chicks were randomly selected from each replicate. The chicks were bled from the jugular vein and then euthanized by dislocation of the neck. After necropsy to check the intestinal bloating, the jejunum and ileum were separated, and the intestinal lesions were scored according to Table 2.

[0047] Table 2 Scoring criteria for intestinal lesions

[0048]

[0049] 1.8 Histopathological observation of jejunum

[0050] Well-fixed jejunal tissue samples were selected, stained with hematoxylin and eosin (HE), and prepared into pathological sections. Subsequently, the pathological changes of the jejunal tissue were observed using an Olympus BX35 biological microscope, and relevant images were acquired.

[0051] 1.9 Measurement of intestinal permeability indicators

[0052] This experiment determined intestinal permeability according to the method reported in the reference. The specific procedures were as follows: Two broiler chickens were randomly selected from each replicate group 2 hours before sampling. Based on the chickens' body weight, 1 mL of FITC-d at a concentration of 4.17 mg / mL was administered orally per kilogram of body weight. After collecting blood from the wing vein, the chickens were euthanized by cervical dislocation. The collected blood samples were centrifuged at 3000 × g for 15 min at 4°C to obtain serum samples. Simultaneously, standard curves were plotted using different concentrations of FITC-d solution. The serum samples were then placed in a multi-mode microplate reader for fluorescence measurement at an excitation wavelength of 485 nm and an emission wavelength of 528 nm, and the fluorescence levels were recorded. Finally, the concentration of FITC-d in the serum samples was calculated based on the plotted standard curves.

[0053] 1.10 Expression of jejunal barrier-related genes

[0054] First, total RNA was extracted from the jejunum using TRIGene Reagent. After extraction, the absorbance of the sample at 260 nm and 280 nm wavelengths was measured using a Multiskan SkyHigh automated microplate reader to determine the purity and concentration of RNA. Subsequently, the RNA was reverse transcribed into cDNA using StarScript III one-tube degenomic reverse transcription premix. Next, following the instructions, the cDNA, primers, and 2×RealStarFast SYBR qPCR Mix were mixed, and the mixture was then placed in a Roche LightCycler 480 II real-time quantitative PCR instrument for quantitative PCR experiments. To ensure the reliability of the experimental results, each sample was tested in triplicate. β-actin was selected as the internal control gene, and the relative expression levels of each gene were calculated using the 2-ΔΔCt method. The primers used in the experiment were manufactured by Beijing Qingke Biotechnology Co., Ltd., and the specific gene primer sequences are shown in Table 3.

[0055] Table 3 Gene Primer Sequences

[0056]

[0057]

[0058] 1.11 Determination of antioxidant performance indicators

[0059] Total antioxidant capacity (T-AOC), superoxide dismutase (SOD), hydrogen peroxide (CAT), and malondialdehyde (MDA) in serum were determined using a kit. The detection methods were based on the instructions of the kit (Nanjing Jiancheng Bioengineering Co., Ltd.).

[0060] 1.12 Measurement of immune function indicators

[0061] The levels of immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) in serum, and interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in jejunal tissue were measured using an ELISA kit. The detection methods were performed according to the kit instructions (Jiangsu Enzyme Immunoassay Co., Ltd.).

[0062] 1.13 Data Statistics and Analysis

[0063] First, the experimental data were organized using Excel (WPS Office 2024), then graphed using GraphPad Prism 9.5 software, and finally, one-way ANOVA was performed using SPSS 20.0 statistical software. Duncan's method was used for multiple comparisons, and significant differences were marked. P < 0.05 was considered significant.

[0064] 2. Experimental Results

[0065] 2.1 Effects of Chuanwu Houhe Decoction on Growth Performance of Broilers Infected with Necrotic Enteritis

[0066] like Figure 1 As shown, compared with the NC group, the NE group had significantly lower average daily feed intake and average daily weight gain, and significantly higher feed conversion ratio (P<0.05). Compared with the NE group, the BMD and HC groups had significantly higher average daily weight gain (P<0.05) and significantly lower feed conversion ratio (P<0.05).

[0067] 2.2 Effect of Chuanwu Houhe Decoction on Intestinal Lesion Score in Broilers Infected with Necrotic Enteritis

[0068] like Figure 2 As shown, compared with the NC group, all groups exhibited varying degrees of intestinal lesions in the jejunum and ileum, with the NE group showing the most severe lesions and numerous hemorrhagic plaques. The BMD group showed the mildest intestinal lesions. With increasing concentrations of Chuanwu Houhe Decoction, the intestinal lesions in the compound treatment groups gradually lessened. Compared with the NC group, the jejunal and ileal lesion scores in the NE group were significantly increased (P<0.05). Compared with the NE group, the jejunal lesion scores in all groups were significantly decreased (P<0.05); the ileal lesion score in the LC group showed no significant decrease (P>0.05), while the ileal lesion scores in the other groups were significantly decreased (P<0.05). This indicates that Chuanwu Houhe Decoction can alleviate the symptoms of necrotic enteritis in broilers.

[0069] 2.3 Effects of Chuanwu Houhe Decoction on the Morphology of Jejunal Tissue in Broilers Infected with Necrotic Enteritis

[0070] like Figure 3 As shown: The jejunal villi structure in the NC group was relatively intact and neatly arranged, with no obvious damage. In the NE group, the jejunal integrity was severely disrupted, with obvious villi breakage, dissolution, and shedding. Compared to the NE group, with increasing concentration of Chuanwu Houhe Decoction, the villi breakage, dissolution, and damage in the Chuanwu Houhe Decoction-treated group gradually improved. The intestinal damage and villi breakage in the BMD group were better than those in the Chuanwu Houhe Decoction-treated group. This study indicates that Chuanwu Houhe Decoction can, to some extent, repair intestinal damage in broilers infected with necrotizing enteritis.

[0071] 2.4 Effects of Chuanwu Houhe Decoction on Intestinal Permeability in Broilers Infected with Necrotic Enteritis

[0072] Intestinal permeability is measured by serum concentration of fluorescein isothiocyanate (FITC-d). Figure 4 As shown, compared with the NC group, the serum FITC-d concentration in the NE group was significantly increased (P<0.05). After drug treatment, compared with the NE group, the serum FITC-d concentration in the LC group, MC group, HC group and BMD group were all significantly decreased (P<0.05).

[0073] 2.5 Effect of Chuanwu Houhe Decoction on the relative expression levels of jejunal barrier-related genes in broilers infected with necrotizing enteritis

[0074] like Figure 5 As shown, compared with the NC group, the mRNA expression levels of Claudin-1, Occludin, ZO-1, and Mucin-2 were significantly decreased in the NE group (P<0.05). Compared with the NE group, the mRNA expression level of ZO-1 was significantly increased in the MC, HC, and BMD groups (P<0.05), and the mRNA expression level of Mucin-2 was significantly increased in the LC, MC, HC, and BMD groups (P<0.05).

[0075] 2.6 Effects of Chuanwu Houhe Decoction on Antioxidant Properties of Broilers Infected with Necrotic Enteritis

[0076] Antioxidant indicators can reflect the body's ability to withstand oxidative stress; by detecting these indicators, the antioxidant levels of broilers can be analyzed. For example... Figure 6 As shown, compared with the NC group, the activities of T-AOC, CAT, and SOD in the serum of the NE group were significantly decreased (P<0.05), while the content of MDA in the serum was significantly increased (P<0.05). After drug treatment, compared with the NE group, the activities of T-AOC, CAT, and SOD in the serum of the HC and BMD groups were significantly increased (P<0.05), while the content of MDA in the serum of the MC, HC, and BMD groups was significantly decreased (P<0.05).

[0077] 2.7 Effects of Chuanwu Houhe Decoction on the Immune Function of Broilers Infected with Necrotic Enteritis

[0078] Detecting pro-inflammatory and anti-inflammatory factors can effectively reflect the degree of inflammation in broilers and can also be used to evaluate the anti-inflammatory efficacy of drugs. Figure 7As shown, compared with the NC group, the concentrations of pro-inflammatory factors IL-1β, IL-6, and TNF-α in the jejunal tissue of the NE group were significantly increased (P<0.05), and the concentration of anti-inflammatory factor IL-10 was also significantly increased (P<0.05). This indicates that after inflammation occurs, the levels of corresponding anti-inflammatory factors in broilers also increase to counteract the inflammation. After drug treatment, compared with the NE group, the concentrations of pro-inflammatory factors IL-1β, IL-6, and TNF-α in the jejunal tissue of the LC, MC, HC, and BMD groups were significantly decreased (P<0.05), while the concentration of anti-inflammatory factor IL-10 was significantly increased (P<0.05). Compared with the NC group, the serum concentrations of IgA, IgG, and IgM in the NE group were significantly decreased (P<0.05). After drug treatment, compared with the NE group, the serum concentrations of IgA, IgG, and IgM in the LC, MC, HC, and BMD groups were all significantly increased (P<0.05).

[0079] In conclusion, Chuanwu Houhe Decoction has a good therapeutic effect on necrotic enteritis in chickens. It can improve the growth performance, immune function and antioxidant capacity of broilers infected with necrotic enteritis, and can repair their intestinal barrier to a certain extent. The therapeutic effect is better when the high dose of Chuanwu Houhe Decoction is used.

Claims

1. A traditional Chinese medicine composition, characterized in that, The ingredients are as follows, in parts by weight: 70-80 parts Andrographis paniculata, 60-65 parts Prunus mume, 55-60 parts Magnolia officinalis, 40-50 parts Areca catechu, 40-45 parts Agrimonia pilosa, and 10-15 parts Glycyrrhiza uralensis.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The ingredients are as follows, in parts by weight: 75 parts Andrographis paniculata, 60 parts dried plum, 60 parts Magnolia officinalis, 45 parts Areca catechu, 45 parts Agrimonia pilosa, and 15 parts Glycyrrhiza uralensis.

3. A method for preparing the traditional Chinese medicine composition according to claim 1 or 2, characterized in that, Includes the following steps: Soak the raw materials in 10 times their weight of distilled water for 12 hours; boil and simmer for 1 hour, then filter the liquid; add 8 times their weight of distilled water to the dregs, boil and simmer for 1 hour, then filter the liquid. The third decoction step is the same as the second; the decoction obtained from the three filtrations is combined and concentrated using a rotary evaporator to make the final concentration of the decoction 1g / mL, thus obtaining the traditional Chinese medicine composition.

4. The use of a traditional Chinese medicine composition prepared according to the method of claim 3 in the preparation of a drug for treating necrotizing enteritis in chickens.

5. The application according to claim 4, characterized in that, The applications include their use in the preparation of drugs to improve the growth performance of broilers infected with necrotizing enteritis.

6. The application according to claim 4, characterized in that, The applications include their use in the preparation of drugs that enhance the immune function of broilers infected with necrotizing enteritis.

7. The application according to claim 4, characterized in that, The applications include their use in the preparation of drugs that enhance the antioxidant properties of broilers infected with necrotizing enteritis.

8. The application according to claim 4, characterized in that, The applications include the use in the preparation of drugs for repairing the intestinal barrier in broilers infected with necrotizing enterocolitis.

9. The application according to claim 4, characterized in that: The necrotizing enteritis in chickens was caused by a mixed infection of Clostridium perfringens type A and Eimeria giantiformis.

10. The application according to claim 4, characterized in that: The medication for treating necrotic enteritis in chickens is administered orally at a dosage of 5-15 mL / L of water.

Images