A plant lyophilized composition having a gut health conditioning function

Abstract

The application relates to the field of intestinal regulation, and specifically discloses a plant freeze-dried composition with intestinal health regulation function. The freeze-dried composition is prepared from the following raw materials: inulin, galactooligosaccharide, resistant dextrin, psyllium husk powder, hawthorn extract, ginger extract, Hericium erinaceus freeze-dried powder, fruit and vegetable powder and freeze-dried probiotic powder. The preparation method comprises the following steps: S1, inulin, galactooligosaccharide, resistant dextrin and psyllium husk powder are weighed according to the weight part and mixed to obtain premix A; S2, the hawthorn extract, the ginger extract and the Hericium erinaceus freeze-dried powder are added into A and mixed to obtain premix B; S3, the freeze-dried probiotic powder is added into B to obtain final mixture C; S4, C is freeze-dried; and S5, the freeze-dried crude product is crushed and sieved. The plant freeze-dried composition can be used for daily regulation of people with intestinal function disorder and maintenance of intestinal microecological balance in a sub-health state, and has the advantages of synergistic regulation, efficient retention of active ingredients and high stability.

Description

Technical Field

[0001] This application relates to the field of intestinal conditioning technology, and more specifically, it relates to a plant freeze-dried composition with intestinal health conditioning function. Background Technology

[0002] A probiotic agent for regulating the level of the intestinal hormone GLP-1 is disclosed in CN116200310B. The probiotic agent comprises *Lactobacillus rhamnosus* LRa05 strain (CGMCC No. 1.12734) and *Lactobacillus reuteri* LR08 strain (CGMCC No. 1.12733), with a live bacteria ratio of 1:3 to 3:1. It may also contain lyophilization protectants and / or prebiotics. The dosage forms include lyophilized powder, capsules, tablets, or granules. This probiotic agent can significantly increase GLP-1 expression in small intestinal endocrine cells, increase GLP-1 expression in intestinal tissue of obese individuals, reduce weight in obese individuals, and reduce serum ALT and AST levels in obese individuals. Furthermore, the strain has high safety, is unlikely to develop drug resistance, and the preparation process is simple, making it suitable for large-scale industrial production.

[0003] The aforementioned application utilizes a combination of *Lactobacillus rhamnosus* LRa05 and *Lactobacillus reuteri* LR08 to synergistically regulate the level of the intestinal hormone GLP-1, thereby achieving weight control and liver protection. It is suitable for metabolic regulation in obese individuals. While the overall strain screening is rigorous and efficacy verification is sufficient, it does not cover intestinal health during use, lacking optimization of the intestinal flora, physical promotion of intestinal motility, and targeted strengthening of the intestinal immune barrier. Furthermore, its formula only contains basic prebiotics and freeze-drying protectants, which makes the intestines prone to smooth muscle spasms and putrefactive bacteria growth due to the increased flora after use, resulting in limited improvement of common intestinal dysfunction symptoms such as constipation, diarrhea, and bloating. In addition, it lacks a refined staged temperature design and material parameter control for the freeze-drying process, and does not employ nitrogen-protected pulverization and nitrogen-filled packaging technology. As a result, the activity of heat-sensitive components decays rapidly during storage, and the product's stability needs further improvement. Summary of the Invention

[0004] To address the problem that related intestinal conditioning products containing only basic prebiotics and freeze-dried preservatives have limited effectiveness in improving common intestinal dysfunction symptoms such as constipation, diarrhea, and bloating, this application provides a plant-based freeze-dried composition with intestinal health conditioning function.

[0005] This application provides a plant-based freeze-dried composition with intestinal health conditioning function, using the following technical solution: A plant-based freeze-dried composition with intestinal health conditioning function is made from the following raw materials in parts by weight: 20-40 parts inulin, 15-30 parts galactooligosaccharides, 10-25 parts resistant dextrin, 5-15 parts psyllium husk powder, 3-10 parts hawthorn extract, 2-8 parts ginger extract, 1-5 parts hericium erinaceus freeze-dried powder, 1-5 parts fruit and vegetable powder, and 1-3 parts freeze-dried probiotic powder; wherein the freeze-dried probiotic powder contains one or more of Bifidobacterium lactis, Lactobacillus acidophilus, and Lactobacillus rhamnosus.

[0006] By employing the above-mentioned technical solutions, inulin and galactooligosaccharides form a complex prebiotic matrix, which selectively promotes the proliferation of Bifidobacterium and regulates the balance of intestinal flora. Furthermore, resistant dextrin and the water-soluble dietary fiber in psyllium husk powder synergistically increase fecal volume and stimulate intestinal peristalsis. The organic acids contained in hawthorn extract inhibit the growth of putrefactive bacteria by lowering the intestinal pH, while gingerol in ginger extract can relieve intestinal smooth muscle spasms. Since Hericium erinaceus freeze-dried powder contains polysaccharides, it can enhance the intestinal immune barrier by activating macrophages. At the same time, the freeze-dried probiotic powder maintains the activity of bacterial strains under the protection of low-temperature mixing technology, ultimately achieving the effects of intestinal health conditioning through flora regulation, physical excretion promotion, and immune enhancement.

[0007] Preferably, the ratio of live bacteria of Bifidobacterium lactis, Lactobacillus acidophilus, and Lactobacillus rhamnosus in the freeze-dried probiotic powder is in the range of 1:1:1 to 2:1:1, and the total live bacteria count of the freeze-dried probiotic powder is not less than 1.0 × 10⁻⁶. 11 CFU / g.

[0008] By adopting the above technical solution, Bifidobacterium lactis is set as the dominant strain because of its stronger colonization ability in the colon compared to Lactobacillus acidophilus; while Lactobacillus rhamnosus accounts for a proportion no less than that of Lactobacillus acidophilus based on its stronger tolerance to gastric acid; the lower limit of total viable bacteria count ensures that the unit dose reaches the clinically effective concentration, while controlling the proportion range to avoid competitive inhibition of strains.

[0009] Preferably, the fruit and vegetable powder is one or more of apple powder, pineapple powder, and strawberry powder.

[0010] By adopting the above technical solutions, the pectin contained in apple powder ferments in the colon to produce short-chain fatty acids that strengthen the intestinal epithelial barrier; while the bromelain in pineapple powder helps to break down food residues and reduce intestinal putrefaction; and the anthocyanins in strawberry powder reduce intestinal oxidative stress damage by scavenging free radicals, thereby improving the flavor acceptability of the composition and helping to maintain intestinal health.

[0011] Preferably, it also contains 0.5 to 2 parts by weight of vitamin C and 0.1 to 0.5 parts by weight of zinc supplement.

[0012] By adopting the above technical solution, vitamin C acts as an antioxidant to protect the activity of freeze-dried probiotics during storage, while zinc supplements promote intestinal mucosal repair by activating carbonic anhydrase. At the same time, the zinc supplement should be controlled below 0.5 servings to avoid binding with dietary fiber and reducing bioavailability.

[0013] Preferably, the composition has a solubility of not less than 95% in water, and the pH value of the prepared solution is in the range of 5.5-6.5.

[0014] By adopting the above technical solution, the solubility index is achieved by controlling the particle size distribution of raw materials and the proportion of hydrophilic polysaccharides to ensure rapid dispersion and avoid clumping. Within the pH control range, the lower limit of 5.5 can avoid excessive acidification that could damage the probiotic cells, while the upper limit of 6.5 is lower than the pH value required for the reproduction of pathogenic bacteria, thereby achieving the effect of enabling the freeze-dried composition to inhibit the reproduction of pathogenic bacteria.

[0015] Preferably, the moisture content of the composition is not higher than 5%, and the particle size distribution is 80-120 mesh.

[0016] By adopting the above technical solution, the upper limit of moisture content is set according to the GB7101-2015 solid beverage standard to prevent excessive water activity from causing the death of probiotics; the lower limit of 80 mesh ensures that there is no grainy feeling when mixing, and the upper limit of 120 mesh avoids the risk of oxidation due to excessively fine powder. This particle size range is also matched with the pore size of the freeze-drying tray to achieve uniform material distribution.

[0017] A preferred method for preparing a plant-based freeze-dried composition with gut health conditioning function includes the following steps: S1. Weigh out inulin, galactooligosaccharides, resistant dextrin and psyllium husk powder by weight, place them in a mixer and mix at 20-40 r / min at 25-35℃ for 20-40 minutes to obtain premix A; S2. Add hawthorn extract, ginger extract and freeze-dried hericium erinaceus powder to premix A, and continue mixing for 15-25 minutes at a speed of 30-50 r / min in an environment with humidity ≤30% to obtain premix B. S3. Slowly add the freeze-dried probiotic powder to the premix B at 10-15℃ and mix at 15-25r / min for 5-15 minutes to obtain the final mix C. S4. Pre-freeze the final mixture C at -30℃ to -40℃ for 2 to 4 hours, and then freeze-dry it under a vacuum of 10 to 20 Pa to obtain the freeze-dried crude product. S5. The freeze-dried crude product is pulverized to 80-120 mesh using a pulverizer and then sieved to obtain the freeze-dried plant composition.

[0018] By adopting the above technical solution, in stage S1, mixing at 25-35℃ ensures the fluidity of polysaccharide raw materials, while a rotation speed of 20-40r / min avoids static electricity accumulation; in stage S2, humidity control prevents the clumping of highly hygroscopic plant extracts; subsequently, in stage S3, the low-temperature environment slows down the metabolic loss of probiotics; and in stage S4, pre-freezing at -30℃ to -40℃ causes water to form fine ice crystals, reducing cell damage, while a vacuum degree of 10-20Pa ensures that the ice crystals sublimate directly; in stage S5, the particle size is matched to the solubility requirements of the final product, and the edge hardened lumps generated during pre-freezing are removed by sieving.

[0019] Preferably, in step S1, the bulk density of premix A is controlled to be 0.35-0.45 g / mL; in step S3, the temperature of final mix C is controlled to be no higher than 15℃.

[0020] By adopting the above technical solution, the lower limit of the bulk density of premix A is 0.35 g / mL to ensure that there is enough space to accommodate the extract powder during subsequent mixing, while the upper limit is controlled at 0.45 g / mL to prevent excessive compaction from affecting the freeze-drying efficiency; at the same time, the upper limit of the temperature of the final mix C is 15℃ based on the critical growth temperature of Lactobacillus acidophilus of 16℃, to avoid short-term temperature rise from activating dormant bacteria and causing premature depletion of activity.

[0021] Preferably, in step S4, the freeze-drying process adopts a staged heating method: the first stage is maintained at -10℃ for 4 to 6 hours, the second stage is maintained at 0℃ for 3 to 5 hours, and the third stage is maintained at 15-20℃ for 8 to 10 hours; and the material thickness is controlled at 8 to 15 mm throughout the entire drying process.

[0022] By adopting the above technical solution, the first stage at -10℃ allows the residual bound water to evaporate slowly without damaging the dried structure; the second stage at 0℃ promotes the decomposition and adsorption of colloidal water; the third stage at 15-20℃ accelerates molecular diffusion to complete the final dehydration; at the same time, the thickness is controlled at a lower limit of 8mm to ensure the drying rate, and an upper limit of 15mm to avoid moisture residue in the center, thus ensuring drying efficiency while controlling the loss of probiotic survival rate.

[0023] In summary, this application has the following beneficial effects: 1. This application uses inulin, galactooligosaccharides, and resistant dextrin to construct a complex prebiotic matrix. Inulin specifically promotes the proliferation of Bifidobacteria, galactooligosaccharides enhance the metabolic activity of the gut microbiota, and resistant dextrin prolongs the fermentation cycle. The three components form a gradient fermentation in the colon. At the same time, the water-soluble fiber of psyllium husk powder increases the water-holding capacity of feces, while the organic acids from hawthorn extract and gingerol from ginger extract inhibit the activity of putrefactive bacteria and relieve intestinal spasms, respectively. Furthermore, Hericium erinaceus polysaccharides directionally activate intestinal immune cells. Combined with the colonization of freeze-dried probiotics, the final result is the optimization of the intestinal microbiota structure, the enhancement of intestinal motility, and the strengthening of the intestinal immune barrier.

[0024] 2. In this application, a strain ratio with an increased proportion of Bifidobacterium lactis is preferred. This is because the colonization advantage of Bifidobacterium lactis and the gastric acid tolerance of Lactobacillus rhamnosus complement each other. In addition, vitamin C scavenge free radicals and inhibit bacterial oxidative damage during freeze-drying, while zinc activates the intestinal mucosal repair enzyme system. At the same time, the total number of live bacteria is controlled to be no less than the clinically effective threshold. This results in an increased survival rate of probiotics in the freeze-dried composition during the digestive tract delivery process, and an increased concentration of short-chain fatty acids, which are metabolites after colonization. This, in turn, reduces the intestinal pH and inhibits the growth of intestinal pathogens.

[0025] 3. The preparation method of this application inhibits the pre-activation of probiotics through low-temperature mixing and utilizes deep pre-freezing at -30℃ to -40℃ to form ice crystals to reduce mechanical damage to cells; in the staged temperature rise freeze drying: the -10℃ stage allows the bound water to be slowly removed to avoid structural collapse, the 0℃ stage dissociates the colloidal bound water to maintain the porous framework, and the 15-20℃ stage accelerates diffusion without damaging the heat-sensitive components. Combined with the steam permeation efficiency controlled by the material thickness, the effect of improving the activity retention rate of the heat-sensitive components is ultimately achieved. Attached Figure Description

[0026] Figure 1 This is a flowchart illustrating a method for preparing a plant freeze-dried composition with intestinal health conditioning function proposed in this application. Detailed Implementation

[0027] The present application will be further described in detail below with reference to the accompanying drawings and embodiments.

[0028] Technical concept In this application, the freeze-dried composition is made from the following raw materials: inulin, galactooligosaccharides, resistant dextrin, psyllium husk powder, hawthorn extract, ginger extract, Hericium erinaceus freeze-dried powder, fruit and vegetable powder, and freeze-dried probiotic powder; the preparation method is as follows: S1, weigh inulin, galactooligosaccharides, resistant dextrin and psyllium husk powder according to the weight parts and mix them to obtain premix A; S2, add hawthorn extract, ginger extract and Hericium erinaceus freeze-dried powder to A and mix to obtain premix B; S3, add freeze-dried probiotic powder to B to obtain final mix C; S4, freeze-dry C; S5, pulverize and sieve the freeze-dried crude product.

[0029] This application utilizes inulin, galactooligosaccharides, and resistant dextrin to construct a complex prebiotic matrix. Inulin specifically promotes the proliferation of Bifidobacteria, galactooligosaccharides enhance the metabolic activity of the gut microbiota, and resistant dextrin prolongs the fermentation cycle, with the three components forming a gradient fermentation process in the colon. Simultaneously, the water-soluble fiber from psyllium husk powder increases fecal water retention, while the organic acids from hawthorn extract and gingerol from ginger extract inhibit the activity of putrefactive bacteria and relieve intestinal spasms, respectively. Furthermore, Hericium erinaceus polysaccharides directionally activate intestinal immune cells. Combined with the colonization of freeze-dried probiotics, the final result is an optimized intestinal microbiota structure, improved intestinal motility, and strengthened intestinal immune barrier.

[0030] Preparation Example 1 Preparation of freeze-dried probiotic powder: *Bifidobacterium lactis*, *Lactobacillus acidophilus*, and *Lactobacillus rhamnosus* strains were inoculated into MRS liquid medium at a live bacteria ratio of 1:1:1 and cultured at 37℃ for 18 hours. The bacterial sludge was then collected by centrifugation, mixed, and resuspended in a freeze-drying protectant containing 80 g / L trehalose and 50 g / L skim milk powder. The total live bacteria concentration of the suspension was adjusted to 2.0 × 10⁻⁶. 12 CFU / mL; after dispensing, pre-freeze at -50℃ for 3 hours, and freeze-dry at a cold trap temperature of -55℃ and a vacuum degree of 15Pa for 24 hours to obtain freeze-dried probiotic powder with a ratio of 1:1:1.

[0031] Preparation Example 2 Preparation of freeze-dried probiotic powder: *Bifidobacterium lactis*, *Lactobacillus acidophilus*, and *Lactobacillus rhamnosus* strains were inoculated into MRS liquid medium at a live bacteria ratio of 1.5:1:1 and cultured at 37℃ for 18 hours. The bacterial sludge was then collected by centrifugation, mixed, and resuspended in a freeze-drying protectant containing 80 g / L trehalose and 50 g / L skim milk powder. The total live bacteria concentration of the suspension was adjusted to 2.0 × 10⁻⁶. 12 CFU / mL; after dispensing, pre-freeze at -50℃ for 3 hours, and freeze-dry at a cold trap temperature of -55℃ and a vacuum degree of 15Pa for 24 hours to obtain freeze-dried probiotic powder with a ratio of 1.5:1:1.

[0032] Preparation Example 3 Preparation of freeze-dried probiotic powder: *Bifidobacterium lactis*, *Lactobacillus acidophilus*, and *Lactobacillus rhamnosus* strains were inoculated into MRS liquid medium at a live bacteria ratio of 2:1:1 and cultured at 37℃ for 18 hours. The bacterial sludge was then collected by centrifugation, mixed, and resuspended in a freeze-drying protectant containing 80 g / L trehalose and 50 g / L skim milk powder. The total live bacteria concentration of the suspension was adjusted to 2.0 × 10⁻⁶. 12 CFU / mL; after dispensing, pre-freeze at -50℃ for 3 hours, and freeze-dry at a cold trap temperature of -55℃ and a vacuum degree of 15Pa for 24 hours to obtain freeze-dried probiotic powder with a ratio of 2:1:1.

[0033] Example 1

[0034] This embodiment provides a plant freeze-dried composition with intestinal health conditioning function, which is made from the following raw materials in parts by weight: 20 parts inulin, 15 parts galactooligosaccharide, 25 parts resistant dextrin, 5 parts psyllium husk powder, 3 parts hawthorn extract, 2 parts ginger extract, 1 part Hericium erinaceus freeze-dried powder, 1 part apple powder, and 1 part freeze-dried probiotic powder; wherein the freeze-dried probiotic powder is the freeze-dried probiotic powder prepared in Preparation Example 1.

[0035] The preparation method of the above-mentioned plant freeze-dried composition with intestinal health conditioning function is as follows: S1. Weigh out inulin, galactooligosaccharides, resistant dextrin and psyllium husk powder by weight, place them in a mixer and mix at 25°C and 20 r / min for 40 min to obtain premix A; The bulk density of premix A is controlled to be 0.35 g / mL; S2. Add hawthorn extract, ginger extract and freeze-dried hericium erinaceus powder to premix A, and continue mixing for 25 minutes at a speed of 30 r / min in an environment with a humidity of 30%RH to obtain premix B. S3. Slowly add the freeze-dried probiotic powder to the premix B at 10°C and mix at 15r / min for 15min to obtain the final mix C. Among them, the temperature of the final mixture C is controlled to be no higher than 10℃; S4. Pre-freeze the final mixture C at -30℃ for 4 hours, and then freeze-dry it under a vacuum of 10Pa to obtain the freeze-dried crude product. The freeze-drying process employs a staged heating method: the first stage maintains -10℃ for 6 hours, the second stage maintains 0℃ for 5 hours, and the third stage maintains 15℃ for 10 hours; and the material thickness is controlled at 8mm throughout the entire drying process. S5. The freeze-dried crude product is pulverized to 80 mesh using a pulverizer and then sieved to obtain the freeze-dried plant composition. The crushing process is protected by nitrogen, the oxygen concentration is controlled to be no higher than 3%, and nitrogen-filled packaging is carried out immediately after crushing, with the residual moisture content in the packaging container not exceeding 3%.

[0036] Example 2

[0037] This embodiment provides a plant-based freeze-dried composition with intestinal health conditioning function, made from the following raw materials in parts by weight: 30 parts inulin, 22 parts galactooligosaccharide, 18 parts resistant dextrin, 10 parts psyllium husk powder, 6 parts hawthorn extract, 5 parts ginger extract, 3 parts Hericium erinaceus freeze-dried powder, 3 parts pineapple powder, 1.2 parts vitamin C, 0.3 parts zinc gluconate, and 2 parts freeze-dried probiotic powder; wherein the freeze-dried probiotic powder is the freeze-dried probiotic powder prepared in Preparation Example 2.

[0038] The preparation method of the above-mentioned plant freeze-dried composition with intestinal health conditioning function is as follows: S1. Weigh out inulin, galactooligosaccharide, resistant dextrin and psyllium husk powder by weight, place them in a mixer and mix at 30°C and 30 r / min for 30 min to obtain premix A; The bulk density of premix A is controlled to be 0.40 g / mL.

[0039] S2. Add hawthorn extract, ginger extract and freeze-dried hericium erinaceus powder to premix A, and continue mixing for 20 minutes at 40 r / min in an environment with 25% RH humidity to obtain premix B. S3. Slowly add the freeze-dried probiotic powder to the premix B at 13°C and mix at 20r / min for 10min to obtain the final mix C. Among them, the temperature of the final mixture C is controlled to be no higher than 13℃.

[0040] S4. Pre-freeze the final mixture C at -35℃ for 3 hours, and then freeze-dry it under a vacuum of 15Pa to obtain the freeze-dried crude product. The freeze-drying process employs a staged heating method: the first stage maintains a temperature of -10℃ for 5 hours, the second stage maintains a temperature of 0℃ for 4 hours, and the third stage maintains a temperature of 18℃ for 9 hours; and the material thickness is controlled at 12mm throughout the entire drying process.

[0041] S5. The freeze-dried crude product is pulverized to 100 mesh using a pulverizer and then sieved to obtain the freeze-dried plant composition. The crushing process is protected by nitrogen, the oxygen concentration is controlled to be no higher than 3%, and nitrogen-filled packaging is carried out immediately after crushing, with the residual moisture content in the packaging container not exceeding 3%.

[0042] Example 3

[0043] This embodiment provides a plant-based freeze-dried composition with intestinal health conditioning function, made from the following raw materials in parts by weight: 40 parts inulin, 30 parts galactooligosaccharide, 10 parts resistant dextrin, 15 parts psyllium husk powder, 10 parts hawthorn extract, 8 parts ginger extract, 5 parts Hericium erinaceus freeze-dried powder, 5 parts strawberry powder, 2 parts vitamin C, 0.5 parts zinc gluconate, and 3 parts freeze-dried probiotic powder; wherein the freeze-dried probiotic powder is the freeze-dried probiotic powder prepared in Preparation Example 3.

[0044] The preparation method of the above-mentioned plant freeze-dried composition with intestinal health conditioning function is as follows: S1. Weigh out inulin, galactooligosaccharide, resistant dextrin and psyllium husk powder by weight, place them in a mixer and mix at 35°C and 40 r / min for 20 min to obtain premix A; The bulk density of premix A is controlled to be 0.45 g / mL; S2. Add hawthorn extract, ginger extract and freeze-dried hericium erinaceus powder to premix A, and continue mixing for 15 minutes at a speed of 50 r / min in an environment with a humidity of 20%RH to obtain premix B. S3. Slowly add the freeze-dried probiotic powder to the premix B at 15°C and mix at 25r / min for 5min to obtain the final mix C. Among them, the temperature of the final mixture C shall not exceed 15℃; S4. Pre-freeze the final mixture C at -40℃ for 2 hours, and then freeze-dry it under a vacuum of 20Pa to obtain the freeze-dried crude product. The freeze-drying process employs a staged heating method: the first stage maintains -10℃ for 4 hours, the second stage maintains 0℃ for 3 hours, and the third stage maintains 20℃ for 8 hours; and the material thickness is controlled at 15mm throughout the entire drying process. S5. The freeze-dried crude product is pulverized to 120 mesh using a pulverizer and then sieved to obtain the freeze-dried plant composition. The crushing process is protected by nitrogen, the oxygen concentration is controlled to be no higher than 3%, and nitrogen-filled packaging is carried out immediately after crushing, with the residual moisture content in the packaging container not exceeding 3%.

[0045] Comparative Example 1: Refer to Example 1, except that inulin was not added, and the rest of the formulation and preparation steps are the same as in Example 1.

[0046] Comparative Example 2: Refer to Example 1, except that no galactooligosaccharides were added, while the rest of the formulation and preparation steps were the same as in Example 1.

[0047] Comparative Example 3: Refer to Example 1, except that resistant dextrin was not added, and the rest of the formulation and preparation steps are the same as in Example 1.

[0048] Comparative Example 4: Refer to Example 1, except that hawthorn extract was not added, and the rest of the formula and preparation steps are the same as in Example 1.

[0049] Comparative Example 5: Refer to Example 1, except that no psyllium husk powder was added; otherwise, the contents are the same as in Example 1.

[0050] Comparative Example 6: Refer to Example 1, except that no freeze-dried probiotic powder was added, while the rest of the formula and preparation steps are the same as in Example 1.

[0051] Performance testing Sample preparation: The composition samples prepared in Examples 1-3 and the control samples prepared in Comparative Examples 1-6 were selected. Each sample was sealed and stored for 14 days under the conditions of protection from light, 25°C and humidity ≤30%RH before testing. Before testing, each sample was diluted with 37°C warm water to a 10% mass concentration solution and allowed to stand for 5 minutes before use.

[0052] Probiotic delivery survival rate assay: A three-stage reaction system was established to simulate the human digestive tract environment: The first stage simulated gastric juice, i.e., a pepsin solution with pH 2.0, and was cultured at 37°C with shaking for 2 hours; the second stage simulated small intestinal juice, i.e., a trypsin solution with pH 6.8, and was cultured at 37°C with shaking for 4 hours; the third stage was inoculated into an anaerobic medium containing fecal microbiota and cultured at 37°C for 24 hours; after each stage, samples were taken, and the number of viable bacteria was determined by the serial dilution plate count method to calculate the overall survival rate from the oral cavity to the colon; the survival rate was calculated as: (number of viable bacteria in the colon stage / initial number of viable bacteria) × 100%.

[0053] Detection of short-chain fatty acid production: 1g of sample solution was mixed with 10mL of colonic simulated fermentation broth and fermented in an anaerobic incubator at 37℃ for 48 hours. After fermentation, 2mol / L hydrochloric acid was added to terminate the reaction. The supernatant was centrifuged and filtered through a 0.22μm filter membrane. Quantitative analysis was then performed using a gas chromatograph with an FID detector. The injection port temperature was set to 250℃ and the detector temperature to 260℃. The temperature was programmed to rise to 60℃ and hold for 1 min, then increased to 180℃ at a rate of 10℃ / min and held for 5 min. The amount of short-chain fatty acids produced was then measured.

[0054] Intestinal propulsion function test: Sixty rats weighing 180±20g were randomly divided into 10 groups of 6 each. Each rat was administered 0.5mL / 100g body weight of the sample solution by gavage daily for 14 consecutive days. After the last administration, the rats were fasted for 24 hours and then administered 1mL / 100g body weight of 5% activated charcoal suspension by gavage. Twenty minutes later, the distance from the pylorus to the leading edge of the charcoal in the small intestine (propulsion length) and the total length of the small intestine were measured. The intestinal propulsion rate was calculated as: (propulsion length / total small intestine length) × 100%.

[0055] Immune barrier function detection: Colon tissue was taken from rats in test 3. After rinsing the intestinal contents with pre-cooled PBS, the mucosal layer was scraped off. Protein lysis buffer was then added and homogenized on ice. The supernatant was collected by centrifugation. Then, the standard and sample were added to a 96-well plate pre-coated with antibody. After incubation at 37°C for 30 minutes, biotin-labeled antibody was added, followed by TMB colorimetric reaction. The absorbance was measured at 450 nm using an ELISA reader. The amount of secretory immunoglobulin A (sIgA) secreted per unit weight of intestinal mucosa was obtained according to the standard curve.

[0056] Product stability testing: First, 1g of sample was dried at 105℃ to constant weight, and the moisture content was determined using the direct drying method according to GB5009.3. Then, another sample was stored at 37℃ and 75% humidity for 30 days, and the viable count was determined and the retention rate was calculated according to GB4789.35. Solubility testing was performed by adding 10g of sample to 100mL of water at 37℃, stirring magnetically at 300rpm for 5 minutes, allowing it to stand, passing the supernatant through a 0.45μm filter membrane, drying, weighing, and calculating the proportion of insoluble matter. pH stability testing was performed by placing the prepared solution in a 37℃ constant temperature incubator and measuring the pH change using a pH meter over 6 hours.

[0057] The performance parameters of Examples 1-3 and Comparative Examples 1-6 are shown in Tables 1, 2 and 3.

[0058] Table 1: Results of Probiotic Survival Rate and Short-Chain Fatty Acid Production During Gastrointestinal Delivery Table 2: Results of intestinal propulsion function and immune barrier function tests Table 3: Product Stability Parameter Test Results Example Conclusion: Based on Examples 1-3 and Comparative Example 1, and in conjunction with Tables 1 and 2, it can be seen that the addition of inulin maintains the integrity of the product's complex prebiotic system. The lack of inulin weakens the specific proliferative stimulation of Bifidobacteria, reduces the efficiency of colonic gradient fermentation, and thus affects the survival and colonization of probiotics in the digestive tract and the generation of short-chain fatty acid metabolites, ultimately resulting in an overall decline in intestinal propulsion function and immune barrier level. The complex prebiotic matrix synergistically constructed by inulin, galactooligosaccharides, and resistant dextrin is the basis for achieving positive regulation of the intestinal microecology.

[0059] Based on Examples 1-3 and Comparative Example 2, and in conjunction with Tables 1 and 3, it can be seen that the introduction of galactooligosaccharides (GOS) enhances the metabolic activity and fermentation capacity of the microbial community. Without the addition of GOS, the synergistic effect of the compound prebiotics is weakened, resulting in insufficient production of short-chain fatty acids. This not only reduces the inhibitory effect of colonic acidification on pathogens but also affects the maintenance of probiotic activity during storage, indicating that GOS enhances the functionality and stability of the product.

[0060] Based on Examples 1-3 and Comparative Example 3, and in conjunction with Table 1, it can be seen that resistant dextrin plays a role in prolonging the fermentation cycle and ensuring continuous energy supply to the distal colon in the entire prebiotic combination. The lack of resistant dextrin will cause the carbon source to be depleted too quickly in the anterior part of the intestine, which is not conducive to the colonization and metabolism of probiotics in the middle and posterior parts of the colon, thereby reducing the cumulative production of short-chain fatty acids. This indicates that the combination of resistant dextrin with other prebiotics can achieve gradient fermentation throughout the colon and amplify the conditioning effect on intestinal health.

[0061] Based on Examples 1-3 and Comparative Example 4, and in conjunction with Tables 1 and 2, it can be seen that the active ingredients such as organic acids contained in hawthorn extract have an auxiliary effect on inhibiting putrefactive bacteria in the intestine and improving the gut microbiota structure. The absence of hawthorn extract weakens the competitive suppression of harmful bacteria, resulting in a decrease in the survival rate of probiotics and the production of short-chain fatty acids. At the same time, intestinal propulsion and immune barrier function are also weakened accordingly, reflecting the synergistic effect of plant extracts, prebiotics, and probiotics in purifying the intestinal environment.

[0062] Based on Examples 1-3 and Comparative Example 5, and in conjunction with Tables 2 and 3, it can be seen that psyllium husk powder, through its highly water-holding soluble fiber, increases the water-holding capacity and volume of intestinal contents, playing a leading role in promoting intestinal peristalsis and improving propulsion rate. The lack of this component leads to weakened intestinal propulsion function and reduced sIgA secretion in the intestinal mucosa, indicating that psyllium husk powder not only improves defecation function through physical means, but also indirectly supports the strengthening of the intestinal immune barrier by optimizing the intestinal environment.

[0063] Combining Examples 1-3 and Comparative Example 6 with Tables 1 and 2, it can be seen that freeze-dried probiotic powder, as the source of live bacteria in the product, is the functional component that produces short-chain fatty acids and competitive colonization. Without the addition of freeze-dried probiotic powder, colonic fermentation loses its primary bacterial supply, leading to a decrease in short-chain fatty acid production, and consequently, a decline in intestinal propulsion function and immune barrier levels to near-basal levels. This demonstrates that exogenous supplementation with active probiotics, working synergistically with the complex prebiotic matrix and plant-based active ingredients, is necessary to achieve the overall health benefits of improving intestinal microecology and enhancing intestinal motility and immunity.

[0064] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A plant-based freeze-dried composition with intestinal health conditioning function, characterized in that, It is made from the following raw materials in parts by weight: 20-40 parts inulin, 15-30 parts galactooligosaccharides, 10-25 parts resistant dextrin, 5-15 parts psyllium husk powder, 3-10 parts hawthorn extract, 2-8 parts ginger extract, 1-5 parts freeze-dried Hericium erinaceus powder, 1-5 parts fruit and vegetable powder, and 1-3 parts freeze-dried probiotic powder; wherein the freeze-dried probiotic powder contains one or more of Bifidobacterium lactis, Lactobacillus acidophilus, and Lactobacillus rhamnosus.

2. The plant freeze-dried composition with intestinal health conditioning function according to claim 1, characterized in that, The ratio of live bacteria (Bifidobacterium lactis, Lactobacillus acidophilus, and Lactobacillus rhamnosus) in the freeze-dried probiotic powder ranges from 1:1:1 to 2:1:1, and the total live bacteria count of the freeze-dried probiotic powder is not less than 1.0 × 10⁻⁶. 11 CFU / g.

3. The plant freeze-dried composition with intestinal health conditioning function according to claim 1, characterized in that, The fruit and vegetable powder is one or more of apple powder, pineapple powder, and strawberry powder.

4. The plant freeze-dried composition with intestinal health conditioning function according to claim 1, characterized in that, It also contains 0.5 to 2 parts by weight of vitamin C and 0.1 to 0.5 parts by weight of zinc supplement.

5. The plant freeze-dried composition with intestinal health conditioning function according to claim 1, characterized in that, The composition has a solubility of not less than 95% in water, and the pH value of the prepared solution is in the range of 5.5-6.

5.

6. The plant freeze-dried composition with intestinal health conditioning function according to claim 1, characterized in that, The composition has a moisture content of no more than 5% and a particle size distribution of 80-120 mesh.

7. A method for preparing the freeze-dried plant composition with intestinal health conditioning function according to any one of claims 1-6, characterized in that, Includes the following steps: S1. Weigh out inulin, galactooligosaccharides, resistant dextrin and psyllium husk powder by weight, place them in a mixer and mix at 20-40 r / min at 25-35℃ for 20-40 minutes to obtain premix A; S2. Add hawthorn extract, ginger extract and freeze-dried hericium erinaceus powder to premix A, and continue mixing for 15-25 minutes at a speed of 30-50 r / min in an environment with humidity ≤30% to obtain premix B. S3. Slowly add the freeze-dried probiotic powder to the premix B at 10-15℃ and mix at 15-25r / min for 5-15 minutes to obtain the final mix C. S4. Pre-freeze the final mixture C at -30℃ to -40℃ for 2 to 4 hours, and then freeze-dry it under a vacuum of 10 to 20 Pa to obtain the freeze-dried crude product. S5. The freeze-dried crude product is pulverized to 80-120 mesh using a pulverizer and then sieved to obtain the freeze-dried plant composition.

8. The method for preparing a plant freeze-dried composition with intestinal health conditioning function according to claim 7, characterized in that, In step S1, the bulk density of premix A is controlled to be 0.35–0.45 g / mL; in step S3, the temperature of final mix C is controlled to be no higher than 15℃.

9. A method for preparing a plant freeze-dried composition with intestinal health conditioning function according to claim 7, characterized in that, In step S4, the freeze-drying process adopts a staged heating method: the first stage is maintained at -10℃ for 4 to 6 hours, the second stage is maintained at 0℃ for 3 to 5 hours, and the third stage is maintained at 15-20℃ for 8 to 10 hours; and the material thickness is controlled at 8 to 15 mm throughout the entire drying process.

10. A method for preparing a plant freeze-dried composition with intestinal health conditioning function according to claim 7, characterized in that, In step S5, nitrogen protection is used during the pulverization process to control the oxygen concentration to no higher than 3%; and nitrogen filling and packaging are carried out immediately after pulverization, with the residual moisture content in the packaging container not exceeding 3%.

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