A high-throughput liquid dispensing platform

Abstract

This application discloses a high-throughput liquid preparation platform, relating to the field of liquid handling technology. The platform includes an intelligent liquid preparation system and a high-throughput pipetting workstation. The intelligent liquid preparation system receives liquid preparation requests, generates a liquid formulation matrix containing the correspondence between well locations, components, and volumes, and converts the matrix into an executable pipetting sequence. The high-throughput pipetting workstation includes a housing, a three-dimensional motion module, a carrier module, and a waste pipetting tip collection module. It receives pipetting sequence instructions from the intelligent liquid preparation system and performs liquid aspiration, transfer, discharge, and tip replacement operations. This high-throughput liquid preparation platform reduces manual calculation errors and well location mapping errors, improving liquid preparation efficiency and data accuracy.

Description

Technical Field

[0001] This application relates to the field of liquid processing technology, and in particular to a high-throughput liquid preparation platform. Background Technology

[0002] In liquid formulation design and pipetting procedure setup scenarios, existing pipetting workstations are primarily designed for streamlined sample transfer or liquid dispensing in the current biomedical field. Their core task is usually to transfer samples from one container to another according to manually preset well positions and actions. For automated laboratories in new energy materials, where human intervention is minimal and the operation of multiple functional platforms is mainly controlled collaboratively by intelligent systems, liquid handling tasks are not simply aspiration and dispensing. Instead, based on material composition, molar ratio, mother liquor concentration, target total volume, and well plate position, the intelligent system automatically generates liquid formulations, analyzes and executes a series of flexible liquid preparation actions based on the instrument hardware characteristics, and forms traceability data between experimental data and physical samples based on well position mapping and execution status. This facilitates backtracking and optimization of experimental results, avoiding the technical pain point of unreproducible results due to liquid preparation errors.

[0003] In existing technologies, high-throughput liquid preparation platforms for automated laboratories of new energy materials need to simultaneously meet four requirements: first, they must be able to understand material formulations and automatically convert them into pipetting actions; second, they must be able to maintain high throughput in irregular combination tasks; third, they must be able to interact with robotic devices with low barriers; and fourth, they must output traceable sample data. However, existing conventional pipetting workstations cannot simultaneously meet these requirements. Summary of the Invention

[0004] The purpose of this application is to provide a high-throughput liquid preparation platform to solve the problem of low intelligence in existing liquid preparation platforms.

[0005] To achieve the above objectives, this application provides a high-throughput liquid preparation platform, including: an intelligent liquid preparation system and a high-throughput pipetting workstation;

[0006] The intelligent liquid preparation system is used to receive liquid preparation requests, generate a liquid formulation matrix containing the correspondence between well positions, components, and volumes, and convert the liquid formulation matrix into an executable sequence of pipetting actions.

[0007] The high-throughput pipetting workstation includes a housing, a three-dimensional motion module, a carrier module, and a waste pipette tip collection module. The high-throughput pipetting workstation is used to receive the pipetting action sequence instructions of the intelligent liquid dispensing system and to complete the liquid aspiration, transfer, discharge, and pipette tip replacement operations.

[0008] In some embodiments, during the operation of the high-throughput pipetting workstation, the intelligent dispensing system can record the execution details of each action of the high-throughput pipetting workstation and generate an execution log.

[0009] In some embodiments, the intelligent liquid preparation system has a built-in permutation and combination calculation module. The permutation and combination calculation module can calculate the transfer volume of each component in each well based on the input parameter data and the target total volume and mother liquor concentration, so as to generate a liquid formulation matrix.

[0010] In some embodiments, the intelligent liquid preparation system can plan the liquid preparation path based on the liquid formulation matrix and the hardware parameters of the high-throughput pipetting workstation, and generate a sequence of pipetting actions including aspiration, dispensing, tip changing, and mixing.

[0011] In some embodiments, the housing includes a supporting base plate, side plates, and a hovering door panel, wherein the side plates and the hovering door panel are vertically distributed around the supporting base plate to form a box-like structure.

[0012] In some embodiments, the three-dimensional motion module includes a y-axis motion component, an x-axis motion component, and a z-axis component. The y-axis motion component includes a y-axis slide rail, a y-axis stepper motor, and an x-axis support frame. The y-axis slide rail is mounted on the inner sidewall of the housing, and the x-axis support frame is slidably fitted onto the y-axis slide rail. The y-axis stepper motor is used to drive the x-axis support frame to move along the y-axis. The x-axis motion component includes an x-axis slide rail and an x-axis stepper motor, both of which are mounted on the x-axis support frame. The z-axis component is slidably disposed on the x-axis slide rail, and the x-axis stepper motor is used to drive the z-axis component to move along the x-axis.

[0013] In some embodiments, the z-axis assembly includes a pitch-changing unit, a z-axis mounting bracket, a z-axis slide rail, a z-axis lead screw motor, an adapter plate, and an air pipetting pump. The pitch-changing unit includes a pitch-changing mounting bracket, a pitch-changing slide rail, and a pitch-changing lead screw motor. The pitch-changing mounting bracket is slidably fitted to the x-axis slide rail. The pitch-changing slide rail and the pitch-changing lead screw motor are both mounted on the pitch-changing mounting bracket. The z-axis mounting bracket is mounted on the pitch-changing mounting bracket. There are two z-axis slide rails, one of which is fixedly mounted on the pitch-changing slide rail, and the other is slidably fitted to the pitch-changing slide rail. Two z-axis lead screw motors are respectively mounted on the two z-axis slide rails. Two adapter plates are respectively slidably fitted to the two z-axis slide rails. Each adapter plate is equipped with an air pipetting pump.

[0014] In some embodiments, the carrier module includes a carrier base plate and multiple sets of functional carriers. The carrier base plate is located above the support base plate and has positioning holes. All sets of functional carriers can be detached and fixed to the positioning holes.

[0015] In some embodiments, the waste gun head collection module includes a collection port, a collection channel, and a waste gun head box. The collection port is located on the base plate of the carrier, and the waste gun head box is suspended at the bottom of the support base plate. One end of the collection channel is connected to the collection port, and the other end is connected to the waste gun head box.

[0016] In some embodiments, the bottom of the support base plate is provided with support feet.

[0017] Compared to the aforementioned background technology, the high-throughput liquid preparation platform provided in this application includes an intelligent liquid preparation system and a high-throughput pipetting workstation. The intelligent liquid preparation system is used to receive liquid preparation requirements, generate a liquid formulation matrix containing the correspondence between well positions, components, and volumes, and convert the liquid formulation matrix into an executable pipetting action sequence. The high-throughput pipetting workstation includes a housing, a three-dimensional motion module, a carrier module, and a waste pipetting tip collection module. The high-throughput pipetting workstation is used to receive the pipetting action sequence instructions from the intelligent liquid preparation system and complete the liquid aspiration, transfer, discharge, and tip replacement operations.

[0018] The intelligent liquid preparation system serves as the control core of the platform. It receives liquid preparation requests from laboratory personnel, automatically performs component combination calculations, ratio conversions, volume calculations, and well mapping, generating a liquid formulation matrix containing well coordinates, component information, and a one-to-one volume correspondence between each component. This matrix is ​​further parsed and broken down into a sequence of pipetting actions executable by the pipetting equipment, which is then sent to the pipetting workstation. The high-throughput pipetting workstation is the execution entity for the liquid preparation operation. After receiving the pipetting action sequence instructions from the intelligent liquid preparation system, the workstation uses a 3D motion module to precisely position the pipetting unit, automatically completing liquid aspiration, transfer, quantitative discharge, and related operations such as automatic pipetting tip replacement and solution mixing, thus achieving liquid preparation. This high-throughput liquid preparation platform automates all tasks related to formulation design, volume conversion, and motion planning, eliminating the need for manual calculation of pipetting volumes per well and manual writing of pipetting programs. This reduces errors from manual conversion and well mapping, thereby improving liquid preparation efficiency and data accuracy. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this application. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the forward structure of the high-throughput liquid preparation platform according to an embodiment of this application;

[0021] Figure 2 This is a schematic diagram of the back structure of the high-throughput liquid preparation platform according to an embodiment of this application;

[0022] Figure 3 This is a schematic diagram of the structure of the three-dimensional motion module according to an embodiment of this application;

[0023] Figure 4 This is a schematic diagram of the forward structure of the z-axis component according to an embodiment of this application;

[0024] Figure 5 This is a schematic diagram of the z-axis component of an embodiment of this application from another perspective;

[0025] Figure 6 This is a schematic diagram of the back structure of the z-axis assembly according to an embodiment of this application;

[0026] Figure 7 This is a schematic diagram of the carrier module according to an embodiment of this application;

[0027] Figure 8 This is a logical framework diagram for generating the liquid formulation matrix and pipetting action sequence of the intelligent liquid preparation system according to an embodiment of this application.

[0028] in:

[0029] 100. Housing; 110. Support leg; 120. Support base plate; 130. Suspended door panel; 140. Maintenance door;

[0030] 200. 3D motion module; 210. Y-axis motion component; 211. Y-axis slide rail; 212. Y-axis stepper motor; 213. X-axis support frame; 220. X-axis motion component; 221. X-axis slide rail; 222. X-axis stepper motor; 230. Z-axis component; 231. Pitch control unit; 2311. Pitch control bracket; 2312. Pitch control slide rail; 2313. Pitch control screw motor; 232. Z-axis bracket; 233. Z-axis slide rail; 234. Z-axis screw motor; 235. Adapter plate; 236. Air pipette pump;

[0031] 300. Carrier module; 310. Carrier base plate; 320. Functional carrier;

[0032] 400. Waste gun head collection module; 410. Collection port; 420. Collection channel; 430. Waste gun head box; 431. Ramp. Detailed Implementation

[0033] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0034] To enable those skilled in the art to better understand the present application, the present application will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0035] It should be noted that the directional terms such as "upper end," "lower end," "left side," and "right side" mentioned below are defined based on the accompanying drawings in the instruction manual.

[0036] like Figures 1 to 8 As shown in the embodiments of this application, the high-throughput liquid preparation platform includes an intelligent liquid preparation system and a high-throughput pipetting workstation. The intelligent liquid preparation system is used to receive liquid preparation requirements, generate a liquid formulation matrix containing the correspondence between well positions, components, and volumes, and convert the liquid formulation matrix into an executable pipetting action sequence. The high-throughput pipetting workstation includes a housing 100, a three-dimensional motion module 200, a carrier module 300, and a waste pipetting tip collection module 400. The high-throughput pipetting workstation is used to receive the pipetting action sequence instructions from the intelligent liquid preparation system and complete the liquid aspiration, transfer, discharge, and pipetting tip replacement operations.

[0037] Specifically, the intelligent liquid preparation system serves as the control core of the platform. It receives liquid preparation requests from lab personnel, automatically performs component combination calculations, ratio conversions, volume calculations, and well mapping, generating a liquid formulation matrix containing well coordinates, component information, and a one-to-one volume correspondence between each component. This matrix is ​​further parsed and broken down into a sequence of pipetting actions executable by the pipetting equipment, which is then sent to the pipetting workstation. The high-throughput pipetting workstation is the execution entity for the liquid preparation operation. After receiving the pipetting action sequence instructions from the intelligent liquid preparation system, the workstation uses a 3D motion module 200 to precisely position the pipetting unit, automatically completing liquid aspiration, transfer, quantitative discharge, as well as automatic pipetting tip replacement, solution mixing, and other related operations to achieve liquid preparation.

[0038] It is understandable that the high-throughput dispensing platform of this application entrusts the entire work of formula design, volume conversion, and motion planning to an intelligent system automatically, eliminating the need for manual calculation of dispensing volume for each well and manual writing of dispensing programs, thereby reducing manual conversion errors and well mapping errors, and thus improving dispensing efficiency and data accuracy.

[0039] In some embodiments, during the operation of a high-throughput pipetting workstation, an intelligent dispensing system can record the execution details of each action of the high-throughput pipetting workstation and generate an execution log.

[0040] Specifically, the information recorded in the execution log includes the pipetting channel number, the execution timestamp of a single step, the physical location of the current operating vehicle, the coordinates of the target well, the solution composition and ratio of the corresponding well, the set volume and actual operating parameters for aspiration and dispensing, the operating status of the pipetting pump, and pipette tip replacement records.

[0041] Understandably, the execution log can bind each physical sample well to the corresponding liquid formulation, operation process, and equipment status, enabling precise correlation and traceability between samples and data. This facilitates researchers in reviewing the liquid preparation process and troubleshooting anomalies, and can also be directly connected to the subsequent experimental platform of the automated laboratory to provide sample traceability data for calcination, testing, and other processes.

[0042] In some embodiments, such as Figure 8 As shown, the intelligent liquid preparation system has a built-in permutation and combination calculation module. The permutation and combination calculation module can calculate the transfer volume of each component in each well based on the input parameter data and the target total volume and mother liquor concentration, so as to generate a liquid formulation matrix.

[0043] Specifically, the calculations are divided into component generation, proportion generation, volume generation, and liquid formulation matrix generation. In component generation, the experimenter enters parameter data, which can be the total number of mother liquors, mother liquor codes, and the number of components per well. In some other embodiments, the experimenter can write the specific liquid preparation requirements into a detailed unstructured natural language statement and input it into the intelligent liquid preparation system. Subsequently, the system encapsulates the statement to be analyzed as a Prompt and submits it to the pre-trained LLM via API calls. Utilizing its reasoning capabilities, the system parses out the key chemical information in the experimental requirements and outputs structured data conforming to the system's preset format. The system then initially generates a set of component codes for each well. The system then guides the experimenter to select the component arrangement method based on the relationship between the total number of mother liquors and the number of components per well: If the total number of mother liquors equals the number of components per well, the system automatically arranges them in a certain order (such as ascending or descending alphabetical order, periodic law, etc.) to generate a non-proportional serial number-component list; if the total number of mother liquors is greater than the number of components per well, the system automatically enters the permutation and combination logic, guiding the experimenter to choose whether to fix one or more components in each well: if not, the system automatically generates a list; if so, the experimenter inputs the number of components to be fixed and their codes, and the system automatically generates a list. The system then proceeds to the proportion generation step.

[0044] During proportion generation, if all components have fixed proportions, input those fixed proportions and select whether to lock their proportions based on whether the component generation steps include fixed components. If there are no fixed components, the branch for locking fixed component proportions cannot be filled in; the system will automatically arrange and combine components and proportions in a certain order (component proportions from largest to smallest or smallest to largest), and perform normalized calculations to generate a list of components with proportion numbers. If there are fixed components, select whether to lock their proportions. If not, the system will automatically arrange and combine components and proportions to generate a list; if so, input the code of the fixed component whose proportion needs to be locked and its proportion, and the system will automatically arrange and combine other components and proportions to generate a list. If the proportions of all components change, input the upper and lower limit proportions of the changing components and their change step size; the system will then automatically arrange and combine all components and proportions to generate a list. If the system already contains fixed components, and only the proportions of some components change in each well: If only the proportions of certain fixed components need to remain constant, input the code and proportion of the fixed components whose proportions need to be locked, skip the branch for setting the sum of proportions, and directly input the upper and lower limits of the proportions of the changing components and their change steps. The system will automatically generate a list by arranging and combining other components and proportions. If the proportions of certain fixed components need to remain constant, and the sum of the proportions of other components needs to be locked, after locking the fixed components and proportions, continue to input the codes and sums of the proportions of the components whose sum of proportions needs to be locked, then input the upper and lower limits of the proportions of the changing components and their change steps. The system will automatically generate a list by arranging and combining other components and proportions. If only the sum of the proportions of a few components needs to remain constant, skip the branch for setting the fixed components and their proportions, directly input the codes and sums of the proportions of the components whose sum of proportions needs to be locked, then input the upper and lower limits of the proportions of the changing components and their change steps. The system will automatically generate a list by arranging and combining other components and proportions. After manual inspection and fine-tuning, proceed to the volume generation step.

[0045] During volume generation, the experimenter provides the total liquid volume for each well (the same by default) and the concentration unit and value of the mother liquor. The system then intelligently calculates the volume of mother liquor to be added for each well and each component based on the previously generated proportional sequence-component list, generating a complete sequence-component-volume list.

[0046] When generating the liquid formulation matrix, the system first provides selectable reservoir type, orifice plate type, and pipette tip box type based on the total volume of the mother liquor, the total volume of the mother liquor transferred in a single operation, the volume of liquid dispensed in a single operation, and the total volume of liquid per well. The experimenter then selects the required carrier in the GUI according to specific experimental needs. Subsequently, the system automatically maps the well locations to the sequence number-component-volume list based on the selected carrier type, forming a liquid formulation matrix containing the sequence number-component-well location coordinates-volume of each component-total volume.

[0047] Understandably, the automatically calculated formula matrix replaces the manual work of converting tables, thereby shortening the formula design cycle, avoiding volume and ratio errors caused by manual calculation, and ensuring the accuracy of solution preparation.

[0048] In some embodiments, such as Figure 8 As shown, the intelligent liquid preparation system can plan the liquid dispensing path based on the liquid formulation matrix and the hardware parameters of the high-throughput pipetting workstation, and generate a sequence of pipetting actions including liquid aspiration, liquid dispensing, pipette tip changing, and mixing.

[0049] Specifically, the system automatically switches between single-channel fine preparation mode and dual-channel parallel preparation mode. When adjacent wells are for single-volume transfers of the same or different reagents, dual-channel parallel operation is activated to simultaneously add samples to both wells, improving preparation efficiency. When adjacent wells have significant component differences or require strict avoidance of cross-contamination, the system switches to single-channel independent operation to ensure preparation accuracy. Simultaneously, the system optimizes the pipetting path, reducing unnecessary movement and improving preparation accuracy and high-throughput efficiency.

[0050] In some embodiments, such as Figure 1 and Figure 2 As shown, the housing 100 includes a supporting base plate 120, side plates, and a suspended door panel 130. The side plates and door panel are vertically distributed around the supporting base plate 120, thereby forming a box-like structure.

[0051] Specifically, the support base plate 120 is horizontally arranged, and the side plates and the hovering door panel 130 are vertically arranged around the support base plate 120. The hovering door panel 130 is located on the front of the support base plate 120 and has a vertical lifting structure, which can be raised and lowered in the vertical direction to open or close. In addition, each side wall is provided with a maintenance door 140, which can be detachably installed on the corresponding side wall.

[0052] Understandably, when the door is raised, the front of the workstation is completely open, providing ample operating space for the AGV robot's robotic arm. When the door is closed, it forms a closed cavity, reducing the interference of external airflow and dust on the accuracy of pipetting.

[0053] In some embodiments, such as Figure 3As shown, the three-dimensional motion module 200 includes a y-axis motion component 210, an x-axis motion component 220, and a z-axis component 230. The y-axis motion component 210 includes a y-axis slide rail 211, a y-axis stepper motor 212, and an x-axis support frame 213. The y-axis slide rail 211 is installed on the inner side wall of the housing 100. The x-axis support frame 213 is slidably fitted to the y-axis slide rail 211. The y-axis stepper motor 212 is used to drive the x-axis support frame 213 to move along the y-axis. The x-axis motion component 220 includes an x-axis slide rail 221 and an x-axis stepper motor 222. Both the x-axis slide rail 221 and the x-axis stepper motor 222 are installed on the x-axis support frame 213. The z-axis component 230 is slidably disposed on the x-axis slide rail 221. The x-axis stepper motor 222 is used to drive the z-axis component 230 to move along the x-axis.

[0054] Understandably, the y-axis stepper motor 212 drives the x-axis support frame 213 to move, so that the x-axis support frame 213 can move along the y-axis slide rail 211 in the y-axis direction, and the x-axis stepper motor 222 drives the z-axis assembly 230 to move, so that the z-axis assembly 230 can move along the x-axis slide rail 221 in the x-axis direction. A cross slide structure is formed between the y-axis motion assembly 210 and the x-axis motion assembly 220, so that the z-axis assembly 230 can move in the xy plane.

[0055] Based on the above embodiments, such as Figures 3 to 6 As shown, the z-axis assembly 230 includes a pitch unit 231, a z-axis mounting bracket 232, a z-axis slide rail 233, a z-axis lead screw motor 234, an adapter plate 235, and an air pipetting pump 236. The pitch unit 231 includes a pitch mounting bracket 2311, a pitch slide rail 2312, and a pitch lead screw motor 2313. The pitch mounting bracket 2311 is slidably fitted onto the x-axis slide rail 221. The pitch slide rail 2312 and the pitch lead screw motor 2313 are both mounted on the pitch mounting bracket 2311. 11. The z-axis fixed bracket 232 is set on the variable pitch fixed bracket 2311. There are two z-axis slide rails 233. One z-axis slide rail 233 is fixedly set on the variable pitch slide rail 2312, and the other z-axis slide rail 233 is slidably fitted on the variable pitch slide rail 2312. Two z-axis lead screw motors 234 are respectively set on the two z-axis slide rails 233. Two adapter plates 235 are slidably fitted on the two z-axis slide rails 233. Each adapter plate 235 is equipped with an air pipetting pump 236.

[0056] Specifically, the variable pitch slide rail 2312 is arranged along the x-axis direction, one z-axis slide rail 233 is fixedly mounted on the variable pitch slide rail 2312, and the other z-axis slide rail 233 is slidably fitted on the variable pitch slide rail 2312. The two z-axis slide rails 233 are arranged in parallel. The variable pitch screw motor 2313 is used to drive the z-axis slide rail 233 slidably fitted on the variable pitch slide rail 2312 to move along the x-axis direction to adjust the distance between the two z-axis slide rails 233. The z-axis screw motor 234 is used to control the corresponding air pipette pump 236 to move along the z-axis direction.

[0057] It is understandable that the two z-axis slide rails 233, along with their corresponding z-axis lead screw motors 234, adapter plates 235, and air pipetting pumps 236, can constitute a dual-channel variable-pitch pipetting unit. The z-axis slide rails 233 fixed on the variable-pitch slide rails 2312 serve as fixed channels, while the z-axis slide rails 233 slidably mounted on the variable-pitch slide rails 2312 serve as movable channels. Each channel is equipped with a corresponding air pipetting pump 236. The air pipetting pump 236 completes the pipetting operation through suction. The z-axis lead screw motor 234 drives the air pipetting pump 236 to move along the z-axis to bring the air pipetting pump 236 closer to or away from the material. The dual-channel variable-pitch pipetting unit can adapt to orifice plate specifications with different orifice spacings, improving versatility.

[0058] In some embodiments, such as Figure 7 As shown, the carrier module 300 includes a carrier base plate 310 and multiple sets of functional carriers 320. The carrier base plate 310 is located above the support base plate 120. The carrier base plate 310 has positioning holes, and the multiple sets of functional carriers 320 can be detached and fixed to the positioning holes.

[0059] Understandably, the Functional Carrier 320 comes in various types to accommodate different experimental consumables such as pipette tip boxes, microplates, liquid storage tanks, and waste liquid tanks. Researchers can flexibly choose the carrier type, quantity, and location based on specific liquid preparation needs. Positioning holes ensure repeatable positioning accuracy for consumable installation, while the detachable structure facilitates cleaning, replacement, and maintenance.

[0060] In some embodiments, such as Figure 3 As shown, the waste gun head collection module 400 includes a collection port 410, a collection channel 420, and a waste gun head box 430. The collection port 410 is opened on the base plate 310 of the carrier frame, and the waste gun head box 430 is suspended at the bottom of the support base plate 120. One end of the collection channel 420 is connected to the collection port 410, and the other end is connected to the waste gun head box 430.

[0061] Specifically, the collection port 410 is located at the corner of the base plate 310 of the carrier frame and is an opening that penetrates the plate surface. The waste gun head box 430 is a drawer-type pull-out structure. When it is full, it can be pulled out from the side of the equipment to be poured out without opening the equipment cover and without occupying the working area operating space. Inside the waste gun head box 430, a ramp 431 guide structure is provided on one side corresponding to the collection channel 420. The falling waste gun heads will slide along the ramp 431 into the depth of the waste gun head box 430, avoiding the accumulation of waste gun heads directly below the channel.

[0062] In some embodiments, the bottom of the support base plate 120 is provided with support feet 110.

[0063] Understandably, the support feet 110 can raise the height of the equipment and leave sufficient installation space under the support base plate 120, so as to accommodate the waste gun head box 430.

[0064] The high-throughput liquid preparation platform provided in this application embodiment has the following operation steps:

[0065] First, unstructured natural language statements are provided manually and intelligently parsed in the cloud to generate components and proportions, or structured parameters are manually entered to generate components and proportions locally. Then, following the prompts in the GUI, the required parameters are entered, and the intelligent liquid preparation system automatically generates a liquid formulation matrix and a sequence of pipetting actions. After manual review and fine-tuning, the system schedules pipetting workstations and AGV robots to perform liquid handling and material transfer actions.

[0066] The z-axis assembly 230 is moved to the innermost part of the instrument, causing the two z-axis slide rails 233 to engage. This drives the tips of the two air pipette pumps 236, fixed on the corresponding adapter plate 235, to reach their minimum distance, raising the air pipette pumps 236 to their highest point and completing the reset action. At this time, the z-axis assembly 230 can avoid the interaction area between the AGV robot's robotic arm and gripper. Subsequently, the hovering door 130 is raised to its maximum height. According to the actual liquid preparation plan, the AGV robot uses its robotic arm and gripper to place the carried materials onto the corresponding functional carrier 320 as needed.

[0067] Start the y-axis motion assembly 210 and the x-axis motion assembly 220, which will drive the z-axis assembly 230 to move horizontally in the xy plane until the tip of the air pipetting pump 236 moves directly above a row of materials loaded on the target functional carrier 320.

[0068] The variable pitch screw motor 2313 is started, which moves the z-axis slide rail 233 on the variable pitch slide rail 2312, so that the corresponding air pipetting pump 236 moves horizontally in the x-axis direction to the target variable pitch position.

[0069] The two z-axis lead screw motors 234 are started, which causes the corresponding two air pipetting pumps 236 to move to the target height in the z-axis direction.

[0070] The z-axis lead screw motor 234 drives the corresponding air pipette pump 236 to descend slowly until the pipette tip can be securely mounted and easily retracted. Then the z-axis lead screw motor 234 moves in the opposite direction and causes the corresponding air pipette pump 236 to rise along the z-axis to the highest point.

[0071] The y-axis motion assembly 210 and x-axis motion assembly 220 are activated to move the tip of the pipette loaded on the air pipette pump 236 directly above a row of materials loaded on the target functional carrier 320. Then, the z-axis lead screw motor 234 is activated to lower the tip of the pipette pump 236 to the target height. Based on the calculated volume of liquid to be drawn or discharged according to the actual liquid preparation scheme, the air pipette pump 236 draws or discharges the same or different volumes of liquid. Finally, the z-axis lead screw motor 234 is activated to raise the air pipette pump 236 along the z-axis to its highest point, completing one aspiration or dispensing action. This process is then repeated to complete the liquid preparation scheme. It should be noted that in this specification, relational terms such as "first" and "second" are used only to distinguish one entity from several other entities and do not necessarily require or imply any such actual relationship or order between these entities.

[0072] The high-throughput liquid preparation platform provided in this application has been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of this application. The descriptions of the embodiments above are only for the purpose of helping to understand the solution and core ideas of this application. It should be noted that those skilled in the art can make several improvements and modifications to this application without departing from the principles of this application, and these improvements and modifications also fall within the protection scope of the claims of this application.

Claims

1. A high-throughput liquid preparation platform, characterized in that, include: An intelligent liquid preparation system is used to receive liquid preparation requests, generate a liquid formulation matrix containing the correspondence between well locations, components, and volumes, and convert the liquid formulation matrix into an executable sequence of pipetting actions. A high-throughput pipetting workstation includes a housing (100), a three-dimensional motion module (200), a carrier module (300), and a waste pipette tip collection module (400). The high-throughput pipetting workstation is used to receive the pipetting action sequence instructions of the intelligent liquid dispensing system and to complete the liquid aspiration, transfer, discharge, and pipette tip replacement operations.

2. The high-throughput liquid preparation platform according to claim 1, characterized in that, During operation, the intelligent dispensing system can record the execution details of each action of the high-throughput pipetting workstation and generate an execution log.

3. The high-throughput liquid preparation platform according to claim 2, characterized in that, The intelligent liquid preparation system has a built-in permutation and combination calculation module. The permutation and combination calculation module can calculate the transfer volume of each component in each well based on the input parameter data and the target total volume and mother liquor concentration, so as to generate a liquid formulation matrix.

4. The high-throughput liquid preparation platform according to claim 3, characterized in that, The intelligent liquid preparation system can plan the liquid preparation path based on the liquid formulation matrix and the hardware parameters of the high-throughput pipetting workstation, and generate a sequence of pipetting actions including liquid aspiration, liquid dispensing, pipette tip changing, and mixing.

5. The high-throughput liquid preparation platform according to claim 1, characterized in that, The housing (100) includes a supporting base plate (120), side plates and a hovering door plate (130). The side plates and the hovering door plate (130) are vertically distributed on the periphery of the supporting base plate (120) to form a box structure.

6. The high-throughput liquid preparation platform according to claim 1, characterized in that, The three-dimensional motion module (200) includes a y-axis motion component (210), an x-axis motion component (220), and a z-axis component (230). The y-axis motion component (210) includes a y-axis slide rail (211), a y-axis stepper motor (212), and an x-axis support frame (213). The y-axis slide rail (211) is mounted on the inner wall of the housing (100), and the x-axis support frame (213) is slidably fitted to the y-axis slide rail (211). The y-axis stepper motor (212)... 2) The x-axis motion assembly (220) is used to drive the x-axis support frame (213) to move along the y-axis. The x-axis motion assembly (220) includes an x-axis slide rail (221) and an x-axis stepper motor (222). The x-axis slide rail (221) and the x-axis stepper motor (222) are both mounted on the x-axis support frame (213). The z-axis assembly (230) is slidably disposed on the x-axis slide rail (221). The x-axis stepper motor (222) is used to drive the z-axis assembly (230) to move along the x-axis.

7. The high-throughput liquid preparation platform according to claim 6, characterized in that, The z-axis assembly (230) includes a pitch unit (231), a z-axis mounting bracket (232), a z-axis slide rail (233), a z-axis lead screw motor (234), an adapter plate (235), and an air pipetting pump (236). The pitch unit (231) includes a pitch mounting bracket (2311), a pitch slide rail (2312), and a pitch lead screw motor (2313). The pitch mounting bracket (2311) is slidably fitted onto the x-axis slide rail (221). The pitch slide rail (2312) and the pitch lead screw motor (2313) are both located on the pitch mounting bracket (2311). The z-axis mounting bracket (232) is disposed on the variable pitch mounting bracket (2311). There are two z-axis slide rails (233), one of which is fixedly disposed on the variable pitch slide rail (2312), and the other is slidably disposed on the variable pitch slide rail (2312). Two z-axis lead screw motors (234) are respectively disposed on the two z-axis slide rails (233). Two adapter plates (235) are respectively slidably disposed on the two z-axis slide rails (233). Each adapter plate (235) is provided with an air pipetting pump (236).

8. The high-throughput liquid preparation platform according to claim 5, characterized in that, The carrier module (300) includes a carrier base plate (310) and multiple sets of functional carriers (320). The carrier base plate (310) is located above the support base plate (120). The carrier base plate (310) has positioning holes, and the multiple sets of functional carriers (320) can be detached and fixed in the positioning holes.

9. The high-throughput liquid preparation platform according to claim 5, characterized in that, The waste gun head collection module (400) includes a collection port (410), a collection channel (420), and a waste gun head box (430). The collection port (410) is located on the base plate (310) of the carrier frame. The waste gun head box (430) is suspended at the bottom of the support base plate (120). One end of the collection channel (420) is connected to the collection port (410), and the other end is connected to the waste gun head box (430).

10. The high-throughput liquid preparation platform according to claim 9, characterized in that, The bottom of the supporting base plate (120) is provided with supporting feet (110).

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