Sterilization device for producing microbial compound microbial agent

Abstract

The application discloses a sterilization device for producing microbial compound microbial inoculant and relates to the technical field of sterilization of compound microbial inoculant.The sterilization device comprises a high-pressure sterilization tank, a water bath heating chamber is arranged outside the high-pressure sterilization tank, liquid inlet pipes and liquid outlet pipes are arranged on the two sides of the bottom of the high-pressure sterilization tank, a hydraulic drive is installed on the top of the high-pressure sterilization tank, a pressurizing piston is fixedly installed on the telescopic end of the hydraulic drive, a plurality of pressure-resistant pipes are arranged on the bottom of the pressurizing piston, and two semispherical covers capable of being automatically opened and closed are hinged to the bottom of each pressure-resistant pipe.The sensitive bacterial strains are physically protected by the pressure-resistant pipes, the basic microbial inoculant can be sterilized by using the highest strength temperature and pressure conditions in the first stage, and the mixed bacteria can be killed within 30 seconds, at the moment, the bifidobacterium is isolated from the external environment, the survival rate is improved, then the bifidobacterium is released, the total heat exposure time of the bifidobacterium is accurately controlled within 10 seconds, and the survival rate of the bifidobacterium is high.

Description

Technical Field

[0001] This invention relates to the field of sterilization technology for compound microbial agents, and more specifically to a sterilization device for the production of microbial compound microbial agents. Background Technology

[0002] Probiotic beverages and compound probiotic preparations are among the fastest-growing segments of the global functional food market. Compound probiotic preparations, by integrating complementary strains of lactic acid bacteria (Lactobacillus plantarum, Lactobacillus acidophilus), bifidobacteria (BB-12, Bifidobacterium animalis), and Bacillus (Bacillus coagulans), can simultaneously achieve multiple health benefits such as regulating intestinal flora, enhancing immunity, and improving metabolism, representing the core development direction for next-generation probiotic products.

[0003] The sterilization requirements for probiotic products present an inherent contradiction: it must thoroughly eliminate contaminating bacteria, pathogens (E. coli, Salmonella), and putrefactive spores (Bacillus coagulans, Bacillus subtilis) introduced during raw materials and production to ensure the product meets commercial sterility requirements and national food safety standards; simultaneously, it must maximize the preservation of the biological activity of all target probiotics to guarantee the viable count meets standards within the product's shelf life. Ultra-high pressure sterilization technology utilizes Pascal's law, using hydrostatic pressure of 100-600 MPa to disrupt the cell membranes, enzyme structures, and nucleic acid hydrogen bonds of microorganisms, achieving sterilization at room temperature. Contaminating bacteria possess a unique multi-layered structure (spore coat, cortex, core), making their pressure resistance far superior to that of vegetative cells. Different probiotic strains exhibit orders of magnitude differences in pressure tolerance: *Lactobacillus plantarum* can withstand 600 MPa of high pressure and has a high survival rate under ultra-high pressure sterilization conditions, while *Bifidobacterium* has a survival rate of less than 50% under the same time and high pressure conditions. Therefore, traditional room temperature ultra-high pressure sterilization technology can only set parameters based on the most sensitive strains, resulting in the inability to completely kill high-pressure resistant bacteria and spores. Summary of the Invention

[0004] The purpose of this invention is to provide a sterilization device for the production of microbial compound inoculants in order to solve the above problems.

[0005] To achieve the above objectives, the present invention specifically adopts the following technical solution: A sterilization device for the production of microbial compound inoculants includes an autoclave, a water bath heating chamber is provided on the outside of the autoclave, an inlet pipe and an outlet pipe are respectively provided on both sides of the bottom of the autoclave, and a hydraulic drive is installed on the top of the autoclave, with a pressure piston fixedly installed on the telescopic end of the hydraulic drive. The bottom of the pressurizing piston is provided with several pressure-resistant tubes. The bottom of the pressure-resistant tubes is hinged with two hemispherical caps that can open and close automatically. Inside the pressurizing piston, a take-up roller and an unwind roller are rotatably installed. A fixed roll of cloth is wound between the take-up roller and the unwind roller. Several through holes are opened on the fixed roll of cloth. A disposable bursting membrane column is bonded to the bottom of the through holes. The top of the pressurizing piston is provided with several insertion tubes. When the insertion tubes descend, they can bring the disposable bursting membrane column into the pressure-resistant tubes and inject bifidobacteria agent into the disposable bursting membrane column. Inside the pressure-resistant tubes, several extrusion blocks are provided. When the extrusion blocks are completely closed, they can form a complete disc.

[0006] Furthermore, a cross-shaped blasting scratch is made at the bottom of the disposable rupture membrane column.

[0007] Furthermore, the pressure-resistant tube has a hydraulic chamber inside, and a hydraulic piston is slidably connected inside the hydraulic chamber. A bottom ring is fixedly installed at the bottom of the hydraulic piston. Several sliding grooves are opened at the bottom of the bottom ring, and a slider is slidably connected inside the sliding groove. A tension spring is provided between the slider and the inner wall of the sliding groove. The tension spring is used to drive the slider to slide outward of the bottom ring. The extrusion block is fixedly installed at the bottom of the slider. The pressure-resistant tube has a control guide hole inside, and the insertion tube can pass through the control guide hole. The control guide hole is set in three sections. The upper section is trumpet-shaped. The middle section is a scraping sleeve section. When the extrusion block is closed in the scraping sleeve section, the disposable burst membrane column is sealed and extruded together. The bottom section is a clamping section. When the extrusion block is closed in the scraping sleeve section, the extrusion block clamps the disposable burst membrane column. A conical transition is provided between the middle section and the bottom section.

[0008] Furthermore, a lifting drive is fixedly installed inside the pressurizing piston. The telescopic end of the lifting drive passes through the pressurizing piston. A lifting plate is fixedly installed at the bottom of the telescopic end of the lifting drive. Several connecting rods are hinged to the bottom of the lifting plate, and the bottom ends of the connecting rods are hinged to the corresponding hemispherical covers.

[0009] Furthermore, a drive worm gear driven by a servo motor is vertically rotatably mounted inside the pressurizing piston, and a transmission worm wheel is rotatably mounted inside the pressurizing piston. The transmission worm wheel meshes with the drive worm gear, and the transmission worm wheel is connected to the take-up roller via a transmission belt.

[0010] Furthermore, the adjacent pressure-resistant tubes are staggered vertically.

[0011] Furthermore, an installation plate is installed on the telescopic end of the hydraulic drive, and a second lifting drive is fixedly installed at the bottom of the installation plate. A bifidobacteria addition box is fixedly installed at the bottom of the telescopic end of the second lifting drive. Several tubes are arranged at the bottom of the bifidobacteria addition box, and a conduit is provided at the top of the bifidobacteria addition box. The conduit is connected to an external bifidobacteria delivery pump.

[0012] Furthermore, the bottom of the cannula is arc-shaped, and an outlet is provided above the arc-shaped portion.

[0013] Furthermore, both the inlet pipe and the outlet pipe are equipped with high-pressure ball valves.

[0014] The beneficial effects of this invention are as follows: This invention provides full physical protection for sensitive bacterial strains through pressure-resistant tubes. In the first stage, the basic bacterial agent can be sterilized under the highest temperature and pressure conditions, which can kill miscellaneous bacteria within 30 seconds. At this time, Bifidobacteria are isolated from the external environment, and the survival rate is improved. Then, Bifidobacteria are released, and the total heat exposure time of Bifidobacteria is precisely controlled within 10 seconds, resulting in a high survival rate of Bifidobacteria.

[0015] This invention allows the membrane column to explode and release bacterial liquid instantly after the hemispherical cap opens synchronously, eliminating the influence of stirring time on the ultra-high pressure sterilization time of Bifidobacteria, making the time control more precise and the sterilization effect better.

[0016] This invention uses disposable, edible burst membrane columns to completely seal sensitive bacterial strains, ensuring no contact with any equipment components. The membrane columns are discharged with the materials in one go, fundamentally eliminating the risk of cross-contamination between different batches of products and different bacterial strains, and solving the problem of unsanitary corners in reusable components.

[0017] This invention employs a series of extrusion blocks, which can roll the bottom end of the disposable burst membrane column into a stable spherical shape while simultaneously clamping the disposable burst membrane column. Combined with the fixed roll fabric winding, it can automatically separate the disposable burst membrane column, enabling rapid continuous production and high sterilization efficiency. Attached Figure Description

[0018] Figure 1 This is a cross-sectional view of the sterilization device of the present invention; Figure 2 This is a schematic diagram of the structure of the Bifidobacterium addition box of the present invention; Figure 3 This is a schematic cross-sectional view of the pressurized piston structure of the present invention. Figure 1 ; Figure 4 This is a schematic cross-sectional view of the pressurized piston structure of the present invention. Figure 2 ; Figure 5 This is a schematic diagram of the fixed fabric roll structure of the present invention; Figure 6 This is a schematic diagram of the one-time burst membrane column structure of the present invention; Figure 7 This is a schematic cross-sectional view of the pressure-resistant tube structure of the present invention.

[0019] Reference numerals: 1. Autoclave; 2. Inlet pipe; 3. Drain pipe; 4. Hydraulic drive; 41. Mounting plate; 5. Pressurizing piston; 51. Take-up roller; 52. Unwound roller; 53. Drive worm gear; 54. Transmission worm wheel; 55. Lifting drive one; 56. Lifting plate; 57. Connecting rod; 6. Bifidobacterium addition box; 61. Lifting drive two; 62. Guide tube; 63. Insertion tube; 64. Outlet; 7. Pressure-resistant tube; 71. Hemispherical cover; 72. Hydraulic chamber; 73. Hydraulic piston; 74. Bottom ring; 75. Slider; 76. Tension spring; 77. Extrusion block; 78. Control guide hole; 8. Fixing roll cloth; 81. Disposable burst membrane column; 82. Bursting scratch; 9. Water bath heating chamber. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.

[0021] Example 1, as Figures 1-7 As shown, a sterilization device for the production of microbial compound inoculants includes an autoclave 1, a water bath heating chamber 9 is provided on the outside of the autoclave 1, an inlet pipe 2 and an outlet pipe 3 are respectively provided on both sides of the bottom of the autoclave 1, a hydraulic drive 4 is installed on the top of the autoclave 1, and a pressure piston 5 is fixedly installed on the telescopic end of the hydraulic drive 4. The bottom of the pressurizing piston 5 is equipped with several pressure-resistant tubes 7. Two automatically opening and closing hemispherical caps 71 are hinged to the bottom of each pressure-resistant tube 7. Inside the pressurizing piston 5, a take-up roller 51 and an unwinding roller 52 are rotatably mounted. A fixed roll of cloth 8 is wound between the take-up roller 51 and the unwinding roller 52. Several through holes are opened on the fixed roll of cloth 8, and disposable bursting membrane columns 81 are adhered to the bottom of the through holes. Several insertion tubes 63 are installed at the top of the pressurizing piston 5. When the insertion tubes 63 descend, they can bring the disposable bursting membrane columns 81 into the pressure-resistant tubes 7 and inject bifidobacteria agent into the disposable bursting membrane columns 81. The inside of the pressure-resistant tubes 7 is equipped with several extrusion blocks 77, 4-8 in total. When the extrusion blocks 77 are completely closed, they can form a complete disc. Adjacent pressure-resistant tubes 7 are staggered vertically, so that the shock waves generated by the explosion of membrane columns at different positions superimpose, forming a three-dimensional mixed flow field. High-pressure ball valves are installed on both the inlet pipe 2 and the outlet pipe 3, which do not affect high-pressure sterilization when closed.

[0022] Sterilization steps: (1) Separate feeding: Mix the resistant bacteria such as Lactobacillus plantarum and Lactobacillus acidophilus with water, sugar, stabilizer and other excipients evenly to prepare a basic bacterial agent. Pump the basic bacterial agent into the high-pressure sterilization chamber (the space below the pressure piston 5) through the liquid inlet pipe 2 with a feed amount of 95L and reserve 5L space. Then control the insertion tube 63 to descend and bring the folded disposable burst membrane column 81 into the pressure-resistant tube 7. The insertion tube 63 injects the Bifidobacterium agent (sensitive bacterial agent) into the disposable burst membrane column 81. The squeezing block 77 first initially closes up and seals the disposable burst membrane column 81 together. Then the squeezing block 77 descends and pushes down the disposable burst membrane column 81 so that the bottom of the disposable burst membrane column 81 forms a uniform spherical shape.

[0023] Sterilization: The hydraulic drive 4 drives the pressurizing piston 5 to descend, pressurizing to 600MPa at a rate of 120MPa / min. At the same time, heating liquid is introduced into the water bath heating chamber 9, raising the temperature to 70℃ at a rate of 20℃ / min. The bacteria in the first stage basic bacterial agent are killed (2.5-3.0 minutes). The pressure is maintained at 600MPa / 70℃ for 30 seconds to kill the bacteria. Due to the protection of the pressure-resistant tube 7, Bifidobacteria are isolated from the external environment and are not affected. The pressure fluctuation is <±1MPa and the temperature fluctuation is <±0.2℃. After the first stage of sterilization is completed, the control hemispherical cover 71 is opened, exposing the bottom end of the disposable burst membrane column 81 to the high-pressure environment. Under the action of the internal and external pressure difference, the bottom end of the disposable burst membrane column 81 ruptures within 0.01 seconds. The supersonic shock wave generated by the explosion atomizes the bacterial solution into 10-50μm microdroplets. The spherical shock wave fills the entire high-pressure chamber within 0.1 seconds, achieving uniform mixing throughout the entire area. The mixing is completed rapidly in a short time. Then, the pressure is maintained at 600MPa / 70℃ for 10 seconds. Due to the use of explosive instantaneous mixing, the total exposure time of Bifidobacteria can be controlled more accurately, improving the survival rate of Bifidobacteria. Then, the equipment rapidly depressurizes to atmospheric pressure at a rate of 150MPa / min. At the same time, a low-temperature liquid is introduced into the water bath heating chamber 9, which is rapidly cooled to below 10℃ at a rate of 40℃ / min. Finally, the drain pipe 3 is opened to directly deliver the sterilized compound bacterial agent to the aseptic cold filling line.

[0024] Currently, in the sterilization process of compound probiotic agents for probiotic beverages, no single sterilization technology can simultaneously achieve a high rate of elimination of contaminating bacteria and a high survival rate of probiotics; a compromise must be made between the two. This invention, however, effectively achieves both the elimination of contaminating bacteria and the improvement of probiotic survival rate through a first-stage process of protecting sensitive bacteria and eliminating contaminating bacteria, followed by a second-stage process of precise, short-time sterilization, resulting in excellent sterilization performance.

[0025] Example 2, based on the above examples, further includes a cross-shaped blasting scratch 82 at the bottom of the disposable blasting membrane column 81. Four pre-made scratches, each 0.02 mm deep, are evenly distributed on the surface of the disposable blasting membrane column 81, forming a symmetrical cross shape to ensure that when the inner liner reaches the rupture pressure, the four weak points rupture simultaneously, resulting in uniform spraying in four directions and avoiding directional spraying and uneven diffusion caused by a single rupture. The disposable blasting membrane column 81 is made of edible starch-based composite film material; it is edible, and the tiny fragments produced by the explosion are edible and digestible.

[0026] Example 3, based on the above examples, further includes: a hydraulic chamber 72 is provided inside the pressure-resistant tube 7; a hydraulic piston 73 is slidably connected inside the hydraulic chamber 72; a bottom ring 74 is fixedly installed at the bottom of the hydraulic piston 73; several sliding grooves are provided at the bottom of the bottom ring 74; a slider 75 is slidably connected inside the sliding grooves; a tension spring 76 is provided between the slider 75 and the inner wall of the sliding groove; the tension spring 76 is used to drive the slider 75 to slide outward of the bottom ring 74; a compression block 77 is fixedly installed at the bottom of the slider 75; a control guide hole 78 is provided inside the pressure-resistant tube 7; the insertion tube 63 can pass through the control guide hole 78; the control guide hole 78 is divided into three sections: the upper section is trumpet-shaped; the middle section is a scraping sleeve section; when the compression block 77 is closed in the scraping sleeve section, the disposable bursting membrane column 81 is sealed and compressed together; and the bottom section is a clamping section; when the compression block 77 is closed in the scraping sleeve section, the compression block 77 clamps the disposable bursting membrane column 81; and a tapered transition is provided between the middle section and the bottom section.

[0027] This embodiment provides a specific control method for the extrusion block 77. The control steps are as follows: The hydraulic piston 73 descends under hydraulic pressure, which drives the bottom ring 74 to descend. The bottom ring 74 drives the extrusion block 77 to descend. When the extrusion block 77 reaches the upper section, it first closes under the guidance of the inclined plane, slowly closing the disposable burst membrane column 81 from all sides. When the extrusion block 77 moves to the middle section, the disposable burst membrane column 81 is sealed and squeezed together. At this time, it is not completely clamped. Driven by the bottom ring 74, the initially closed extrusion block 77 moves downward in a ring shape, rolling the bottom end of the disposable burst membrane column 81 into a spherical shape. Finally, the extrusion block 77 is inserted into the bottom section, tightly clamping the disposable burst membrane column 81 and completely sealing the control guide hole 78, ensuring that when the hemispherical cover 71 is opened later, there will be no pressure leakage through the control guide hole 78, and the internal pressure of the equipment is stable.

[0028] Example 4, based on the above examples, further includes a lifting drive 55 fixedly installed inside the pressurizing piston 5. The telescopic end of the lifting drive 55 passes through the pressurizing piston 5. A lifting plate 56 is fixedly installed at the bottom of the telescopic end of the lifting drive 55. Several connecting rods 57 are hinged to the bottom of the lifting plate 56. The bottom end of the connecting rods 57 is hinged to the corresponding hemispherical cover 71.

[0029] By controlling the operation of the lifting drive 55 (which can be a pneumatic or hydraulic cylinder), the lifting drive 55 drives the lifting plate 56 to rise, and the lifting plate 56 drives all the hemispherical covers 71 to open simultaneously through the connecting rod 57.

[0030] Example 5, based on the above examples, further includes a drive worm 53 driven by a servo motor vertically rotatably mounted inside the pressure piston 5, and a transmission worm wheel 54 rotatably mounted inside the pressure piston 5. The transmission worm wheel 54 meshes with the drive worm 53, and the transmission worm wheel 54 is connected to the take-up roller 51 via a transmission belt.

[0031] The servo motor drives the drive worm gear 53 to rotate, which in turn drives the transmission worm wheel 54 to rotate. The transmission worm wheel 54 drives the take-up roller 51 to rotate via the transmission belt. The take-up roller 51 takes up the used fixed roll of cloth 8. Since the disposable bursting membrane column 81 is tightly clamped by the extrusion block 77 at this time, the used disposable bursting membrane column 81 is detached from the fixed roll of cloth 8 under the action of the winding force, and the connection is directly pulled off. The new fixed roll of cloth 8 and the folded disposable bursting membrane column 81 rotate back to the position corresponding to the pressure-resistant tube 7. Before the next use, the extrusion block 77 is reset by hydraulic control, and the disposable bursting membrane column 81 is manually pulled out of the pressure-resistant tube 7 to ensure that no bacterial agent remains inside the pressure-resistant tube 7.

[0032] Example 6, based on the above examples, further includes an installation plate 41 mounted on the telescopic end of the hydraulic drive 4, a lifting drive 61 fixedly mounted on the bottom of the installation plate 41, a bifidobacteria addition box 6 fixedly mounted on the bottom of the telescopic end of the lifting drive 61, several insertion tubes 63 are arranged at the bottom of the bifidobacteria addition box 6, and a conduit 62 is arranged at the top of the bifidobacteria addition box 6, the conduit 62 being connected to an external bifidobacteria delivery pump.

[0033] The bottom of the insertion tube 63 is arc-shaped to prevent it from damaging the membrane column 81 during a one-time burst, and an outlet 64 is provided above the arc-shaped part.

[0034] By controlling the operation of the lifting drive 61 (which can be a pneumatic or hydraulic cylinder), the lifting drive 61 drives the bifidobacteria addition box 6 to descend relative to the pressurizing piston 5, and the bifidobacteria addition box 6 drives the insertion tube 63 to be inserted into the pressure-resistant tube 7.

[0035] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A sterilization device for the production of microbial compound inoculants, comprising an autoclave (1), characterized in that, The high-pressure sterilizer (1) is provided with a water bath heating chamber (9) on the outside. The bottom of the high-pressure sterilizer (1) is provided with an inlet pipe (2) and an outlet pipe (3) on both sides respectively. The top of the high-pressure sterilizer (1) is equipped with a hydraulic drive (4). The telescopic end of the hydraulic drive (4) is fixedly equipped with a pressure piston (5). The bottom of the pressurizing piston (5) is provided with several pressure-resistant tubes (7). The bottom of the pressure-resistant tubes (7) is hinged with two hemispherical covers (71) that can open and close automatically. The inside of the pressurizing piston (5) is rotatably installed with a take-up roller (51) and a release roller (52). A fixed roll cloth (8) is wound between the take-up roller (51) and the release roller (52). Several through holes are opened on the fixed roll cloth (8). A disposable bursting membrane column (81) is glued to the bottom of the through holes. Several insertion tubes (63) are provided on the top of the pressurizing piston (5). When the insertion tubes (63) descend, they can bring the disposable bursting membrane column (81) into the pressure-resistant tube (7) and inject bifidobacteria agent into the disposable bursting membrane column (81). Several extrusion blocks (77) are provided inside the pressure-resistant tube (7). When the extrusion blocks (77) are completely closed, they can form a complete disc.

2. The sterilization device for producing microbial compound inoculants according to claim 1, characterized in that, The bottom of the disposable burst membrane column (81) has a cross-shaped burst scratch (82).

3. The sterilization device for producing microbial compound inoculants according to claim 1, characterized in that, The pressure-resistant tube (7) has a hydraulic chamber (72) inside, and a hydraulic piston (73) is slidably connected inside the hydraulic chamber (72). A bottom ring (74) is fixedly installed at the bottom of the hydraulic piston (73). Several sliding grooves are opened at the bottom of the bottom ring (74), and a slider (75) is slidably connected inside the sliding groove. A tension spring (76) is provided between the slider (75) and the inner wall of the sliding groove. The tension spring (76) is used to drive the slider (75) to slide outward of the bottom ring (74). The extrusion block (77) is fixedly installed on the slider (74). At the bottom of 75), the pressure-resistant tube (7) has a control guide hole (78) inside. The insertion tube (63) can pass through the control guide hole (78). The control guide hole (78) is set in three sections. The upper section is flared. The middle section is a scraping section. When the extrusion block (77) is closed in the scraping section, the disposable burst membrane column (81) is sealed and squeezed together. The bottom section is a clamping section. When the extrusion block (77) is closed in the scraping section, the extrusion block (77) clamps the disposable burst membrane column (81). A conical transition is provided between the middle section and the bottom section.

4. The sterilization device for producing microbial compound inoculants according to claim 3, characterized in that, The pressurizing piston (5) is fixedly installed with a lifting drive (55). The telescopic end of the lifting drive (55) passes through the pressurizing piston (5). A lifting plate (56) is fixedly installed at the bottom of the telescopic end of the lifting drive (55). Several connecting rods (57) are hinged to the bottom of the lifting plate (56). The bottom end of the connecting rods (57) is hinged to the corresponding hemispherical cover (71).

5. The sterilization device for producing microbial compound inoculants according to claim 4, characterized in that, The pressure piston (5) has a vertically rotating drive worm (53) driven by a servo motor inside, and a transmission worm wheel (54) is also rotating inside the pressure piston (5). The transmission worm wheel (54) meshes with the drive worm (53), and the transmission worm wheel (54) is connected to the take-up roller (51) via a transmission belt.

6. The sterilization device for producing microbial compound inoculants according to claim 5, characterized in that, The adjacent pressure-resistant pipes (7) are staggered vertically.

7. The sterilization device for producing microbial compound inoculants according to claim 1, characterized in that, An installation plate (41) is installed on the telescopic end of the hydraulic drive (4). A lifting drive (61) is fixedly installed at the bottom of the installation plate (41). A bifidobacteria addition box (6) is fixedly installed at the bottom of the telescopic end of the lifting drive (61). Several tubes (63) are set at the bottom of the bifidobacteria addition box (6). A conduit (62) is set at the top of the bifidobacteria addition box (6). The conduit (62) is connected to an external bifidobacteria delivery pump.

8. The sterilization device for producing microbial compound inoculants according to claim 7, characterized in that, The bottom of the cannula (63) is arc-shaped, and an outlet (64) is provided above the arc-shaped part.

9. A sterilization device for producing microbial compound inoculants according to any one of claims 1-8, characterized in that, High-pressure ball valves are installed on both the inlet pipe (2) and the outlet pipe (3).

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