A sampling device for microbial detection

Abstract

The application relates to the technical field of water body microorganism sampling and detection, and discloses a sampling device for microorganism detection, which comprises a water storage tank body, a pipeline module and a sampling module are respectively arranged on the water storage tank body, the sampling module is connected with external water quality detection equipment, the sampling module comprises multiple sampling tubes and a protective cylinder body, the multiple sampling tubes are arranged in the protective cylinder body and are completely isolated from the water body of the water storage tank body, physical isolation between the sampling tubes and the water body is realized, the cleanliness of the sampling tubes is improved, the problem that the sampling tubes are directly soaked in the water body, microorganisms are prone to adhering and breeding along the tube wall to form a bacterial film in the prior art is solved, exogenous miscellaneous bacteria are prevented from invading the sampling tubes from the source, the original microorganism community of the sampling water sample is prevented from being disturbed, and the accuracy of microorganism detection results is improved; the protective cylinder body is matched with a deep-section thick-wall hole channel structure at a water inlet hole, the path of microorganism climbing along the wall is prolonged, and a double-layer physical antibacterial barrier is formed.

Description

Technical Field

[0001] This invention relates to the field of water microbial sampling and detection technology, specifically to a sampling device for microbial detection. Background Technology

[0002] Microbiological indicators within water bodies such as water supply tanks, underground pump house reservoirs, and surface water tanks are core criteria for assessing water safety. Regular stratified sampling at different depths within these tanks is necessary to detect microbial colonies, bacterial species, and other indicators, accurately identifying potential microbial contamination risks. Sampling equipment must be continuously submerged in the water to achieve stratified sampling at different depths, adapting to the long-term unattended, stable, and continuous sampling and testing requirements of various water tanks.

[0003] In existing water microbial sampling equipment, the sampling pipeline is directly and continuously immersed in the water body to be tested. The inner and outer walls of the pipeline are in constant contact with the water, allowing microorganisms in the water to crawl and attach to the pipeline walls. Even with conventional valve structures, microorganisms can still continuously infiltrate along the valve gaps, eventually forming a dense bacterial film on the pipeline walls. During subsequent sampling, bacteria from this bacterial film can contaminate the water sample, altering the original microbial community structure and directly causing severe distortion in microbial colony counts and species identification results, rendering the test data unreliable.

[0004] Meanwhile, the water in the pool has been stagnant for a long time, and microorganisms and suspended impurities are subject to gravity settling, resulting in a huge difference in the distribution of microbial concentrations at different depths and in different areas of the water. Conventional sampling equipment can only collect local water samples around the equipment, and the water samples collected cannot represent the overall microbial level of the water at that depth. The test results are one-sided and cannot objectively reflect the overall microbial pollution status of the pool, making it difficult to meet the high-precision requirements for safe detection of microorganisms in water.

[0005] Therefore, the present invention proposes a sampling device for microbial detection. Summary of the Invention

[0006] The purpose of this invention is to provide a sampling device for microbial detection, so as to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a sampling device for microbial detection, comprising a water storage tank, on which a pipeline module and a sampling module are respectively installed. The sampling module is connected to an external microbial detection instrument. The sampling module includes multiple sampling tubes and a protective cylinder. The sampling tubes are located inside the protective cylinder and are isolated from the water in the water storage tank by the protective cylinder. The surface of the protective cylinder has water intake holes adapted to the number of sampling tubes. The multiple sampling tubes are arranged corresponding to water intake holes at different heights. Each water intake hole has a depth section to extend the path of microorganisms crawling along the wall and reduce the invasion of microorganisms into the sampling tubes. A movable plug adapted to the number of sampling tubes is slidably sealed on the protective cylinder. The movable plug normally seals the water intake holes and moves during sampling to connect the water storage tank and the sampling tubes.

[0008] Preferably, each of the sampling tubes is individually connected to an external microbial detection instrument. The sampling tubes are adaptable to water absorption sampling mode and can also be connected to backflushing medium to achieve backflushing cleaning, so as to avoid the accumulation of dirt and the growth of bacteria inside the sampling tubes that may clog the pipes.

[0009] Preferably, the protective cylinder is fixedly connected to the water storage tank, and all the sampling tubes are fixedly connected to the protective cylinder. The multiple sampling tubes correspond to water intake holes at different heights, and the water intake holes are staggered on the surface of the protective cylinder.

[0010] Preferably, the water storage tank is equipped with a drive module, which is connected to the movable plug block for driving the movable plug block to move along the protective cylinder, thereby realizing the opening and closing control of the water intake hole.

[0011] Preferably, the drive module includes a motor fixedly connected to the water storage tank, and a drive gear is fixedly connected to the output shaft of the motor.

[0012] Preferably, the protective cylinder is rotatably connected to a screw rod that matches the number of movable plugs. The screw rod is threadedly connected to the movable plugs, and a driven gear is fixedly connected to the top of each screw rod. A driven gear ring is also rotatably connected to the protective cylinder. The drive gear meshes with the outer side of the driven gear ring, and the inner side of the driven gear ring meshes with the driven gear. Thus, the screw rod is driven to rotate synchronously by the rotation of the motor, thereby causing the movable plugs to move axially.

[0013] Preferably, the protective cylinder is rotatably connected to the surface of the water storage tank, and the motor drives the protective cylinder to rotate around its own axis to achieve self-cleaning of the outer wall of the protective cylinder and reduce the adhesion and growth of microorganisms.

[0014] Preferably, a second drive gear is provided at the bottom of the first drive gear, and a toothed groove is provided on the surface of the protective cylinder. The second drive gear meshes with the toothed groove to transmit the rotation of the motor to the protective cylinder, thereby driving the entire protective cylinder to rotate.

[0015] Preferably, a clutch mechanism is installed on the output shaft of the motor. One end of the clutch mechanism is connected to the first drive gear and the other end is connected to the second drive gear. The clutch mechanism is used to control the power supply of the first drive gear and the second drive gear through the clutch action, thereby selectively driving the movable block to move to open and close the water intake hole, or driving the entire protective cylinder to rotate to achieve self-cleaning and antibacterial.

[0016] Preferably, a mounting base is fixedly connected to the water storage tank, and the protective cylinder is rotatably connected to the water storage tank through the mounting base to achieve stable rotation of the protective cylinder.

[0017] Compared with the prior art, the beneficial effects of the present invention are: 1. By setting up a protective cylindrical structure, multiple sampling tubes are built inside and completely isolated from the water in the storage tank. This achieves physical isolation between the sampling tubes and the water, improving the cleanliness of the sampling tubes. It addresses the problem in existing technologies where sampling tubes are directly immersed in water, allowing microorganisms to easily adhere to the tube wall and form a biofilm. This helps prevent external bacteria from contaminating the sampling tubes at the source, ensuring that the original microbial community of the sampled water is not disturbed, and improving the accuracy of microbial detection results. The protective cylindrical structure, combined with the thick-walled channel structure in the deep section at the water intake hole, extends the path for microorganisms to crawl along the wall, forming a double-layer physical antibacterial barrier. Even if a small number of microorganisms bypass the sealing structure, it is difficult for them to spread inward along the deep channel. This is suitable for the long-term immersion of the sampling device in water, providing a basic guarantee for long-term accurate microbial sampling.

[0018] 2. By setting up a movable plug and a sliding seal with the protective cylinder, the water intake hole is sealed under normal conditions and moves to connect the water body and the sampling tube during sampling. This achieves precise opening and closing control of the water intake hole, improves the sealing and controllability of the sampling process, and overcomes the defect of existing valve sealing gaps that are prone to microbial and bacterial contamination of water samples. The movable plug is synchronously driven by the screw, with strong opening and closing synchronization. During the movement process, it can peel off microbial impurities attached to the orifice, reduce the base number of bacteria in the water sample during the sampling period, further ensure the purity of the water sample to be tested, and ensure that the microbial detection data truly reflects the state of the original bacterial community in the water body.

[0019] 3. By setting up an integrated transmission structure with a single motor, gear ring, driven gear, and lead screw, it synchronously drives multiple moving plugs, ensuring stable transmission and strong synchronization. This overcomes the shortcomings of complex independent drive structures with multiple pipelines and their susceptibility to underwater failures, making it suitable for long-term unattended sampling in water tanks. The overall pure mechanical meshing transmission eliminates the risk of corrosion and short circuits in underwater electric control valves, extending the equipment's service life. With a clutch mechanism, it allows for selective switching of power paths, enabling separate stratified sampling and cylinder self-cleaning and antibacterial actions, enhancing the equipment's multi-functional adaptability.

[0020] 4. By setting up a rotatable protective cylinder, the cylinder is driven to rotate at low speed by the meshing of the motor gears, realizing the active self-cleaning of the outer wall of the cylinder, which improves the defects of fixed cylinder microbial enrichment and local water sample bacterial concentration distortion. The slow rotation of the cylinder causes micro-disturbance in the surrounding water, dispersing the deposited microorganisms and suspended impurities, so that the bacterial distribution in the sampling area is uniform, while not destroying the water stratification structure. Water samples collected from each water intake hole at each height can represent the average microbial level of the water at that depth, completely avoiding the detection deviation caused by local bacterial enrichment.

[0021] 5. By matching multiple independent sampling tubes with water intake holes at different heights, independent sampling of water layers can be achieved. Each tube is individually connected to an external microbial detection instrument, eliminating cross-contamination between water samples from different depths. The sampling tubes can be flushed with cleaning media to remove residual water samples and deposited impurities, inhibit the growth and blockage of bacterial films inside the tubes, reduce the frequency of manual maintenance, and are suitable for long-term continuous microbial sampling and detection operations in various water pools. Attached Figure Description

[0022] Figure 1 This is a frontal perspective three-dimensional schematic diagram of the main structure in Embodiment 1 of the present invention; Figure 2 This is a side view of the main structure in Embodiment 1 of the present invention; Figure 3 This is a three-dimensional cross-sectional view of the main structure in Embodiment 1 of the present invention; Figure 4 This is a three-dimensional schematic diagram of the sampling module in Embodiment 1 of the present invention; Figure 5 This is a cross-sectional perspective view of the sampling module in Embodiment 1 of the present invention; Figure 6 This is an exploded perspective view of the sampling module in Embodiment 1 of the present invention; Figure 7 This is a three-dimensional schematic diagram of the water intake hole in a connected state in Embodiment 1 of the present invention; Figure 8 This is a partial three-dimensional schematic diagram of the main structure in Embodiment 2 of the present invention; Figure 9 This is a planar schematic diagram of the sampling module in Embodiment 2 of the present invention; Figure 10 For the present invention Figure 9 Enlarged planar schematic diagram of the structure at point A; In the picture: 1. Water storage tank; 11. Mounting base; 2. Sampling module; 21. Sampling tube; 22. Protective cylinder; 23. Movable plug; 231. Screw; 232. Driven gear ring; 233. Driven gear; 234. Deep section; 3. Piping module; 4. Drive module; 41. Motor; 42. Drive gear one; 43. Clutch mechanism; 44. Drive gear two; 45. Gear groove. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0024] It should be noted that the working principle and specific structure of the above-mentioned clutch mechanism 43 and external microbial detection instrument are existing technologies. The clutch mechanism 43 is used to switch the power path of the motor 41 selectively, and can selectively control the power transmission to the drive gear 1 42 to drive the movable block 23 to move and open and close the water intake hole, or to the drive gear 2 44 to drive the protective cylinder 22 to rotate to achieve self-cleaning and antibacterial effect. The external microbial detection instrument is used to receive the stratified water samples delivered by each sampling tube 21 and complete the detection of indicators such as microbial colonies and bacterial groups in the water. Given the universality of the above structure, its specific principle will not be described in detail below.

[0025] like Figure 1-4 As shown, Example 1: A sampling device for microbial detection includes a water storage tank 1, on which a pipeline module 3 and a sampling module 2 are respectively installed. The sampling module 2 is connected to an external microbial detection instrument. The sampling module 2 includes multiple sampling tubes 21 and a protective cylinder 22. The sampling tubes 21 are located inside the protective cylinder 22 and are isolated from the water in the water storage tank 1 by the protective cylinder 22. The surface of the protective cylinder 22 has water intake holes that are adapted to the number of sampling tubes 21. The multiple sampling tubes 21 are arranged corresponding to water intake holes at different heights. Each water intake hole is provided with a depth section 234 to extend the path of microorganisms crawling along the wall and reduce the invasion of microorganisms into the sampling tubes 21. A movable plug 23 adapted to the number of sampling tubes 21 is slidably sealed on the protective cylinder 22. The movable plug 23 normally seals the water intake holes and moves during sampling to connect the water storage tank 1 and the sampling tubes 21.

[0026] It should be noted that each sampling tube 21 is individually connected to an external microbial detection instrument. The sampling tube 21 can be adapted to the water absorption sampling mode and can also be connected to the backflushing medium to achieve backflushing cleaning, so as to avoid the accumulation of dirt and the growth of bacteria inside the sampling tube 21 that may clog the pipeline.

[0027] like Figure 4-6 As shown, the protective cylinder 22 is fixedly connected to the water storage tank 1, and the sampling tubes 21 are all fixedly connected to the protective cylinder 22. The multiple sampling tubes 21 correspond to water intake holes at different heights, and the water intake holes are staggered on the surface of the protective cylinder 22.

[0028] like Figure 4-6 As shown, a drive module 4 is provided on the water storage tank 1. The drive module 4 is connected to the movable block 23 for transmission and is used to drive the movable block 23 to move along the protective cylinder 22 to realize the opening and closing control of the water intake hole. The drive module 4 includes a motor 41 fixedly connected to the water storage tank 1, and a drive gear 42 is fixedly connected to the output shaft of the motor 41.

[0029] It should be noted that the surface of the protective cylinder 22 is rotatably connected with screw rods 231 that are matched with the number of movable blocking blocks 23. The screw rods 231 are threadedly connected to the movable blocking blocks 23, and the top of each screw rod 231 is fixedly connected with a driven gear 233. A driven gear ring 232 is also rotatably connected to the protective cylinder 22. The drive gear 42 meshes with the outer side of the driven gear ring 232, and the inner side of the driven gear ring 232 meshes with the driven gear 233. Thus, the rotation of the motor 41 drives the screw rods 231 to rotate synchronously, thereby causing the movable blocking blocks 23 to move axially.

[0030] It should be added that this device can be placed inside underground pump room reservoirs, water supply tanks and other water storage bodies for long-term submersion. Under normal static standby conditions, the movable block 23 always seals the water intake hole, preventing water, suspended impurities and microorganisms in the tank from entering the protective cylinder 22. When the preset sampling cycle is reached, the drive module 4 drives the movable block 23 to move axially, opening water intake holes at different heights. The stratified water in the water storage tank 1 flows into the sampling pipe 21 through the depth section 234 to complete the stratified sampling. The depth section 234 is an extended thick-walled channel extending inward from the water intake hole, which greatly extends the distance that microorganisms crawl inward along the wall. The narrow channel physically blocks the invasion of miscellaneous bacteria into the sampling tube 21. Each sampling tube 21 is independently connected to an external microbial detection instrument, and the stratified water samples do not mix or cross-contaminate, accurately restoring the original microbial state of water bodies at different depths. After sampling, a cleaning medium can be introduced into the sampling tube 21 for backwashing to remove residual water samples and impurities in the tube and inhibit the formation of bacterial film and blockage in the pipeline.

[0031] It should be added that this device is completely submerged in the water body to be tested for a long time. Compared with short-term immersion sampling equipment, the risk of continuous microbial contamination along the wall is higher under long-term immersion conditions. Conventional sampling tube 21 has no isolation protection, and water microorganisms continuously adhere to the tube wall to form a bacterial film. When sampling, the bacteria in the bacterial film mix into the water sample, directly changing the original microbial composition of the water sample, resulting in a significant distortion of the colony count and bacterial species identification results. Conventional electric valves are prone to corrosion, short circuits, and sealing failure when submerged in water for a long time, and cannot effectively block microorganisms, making it difficult to meet the needs of long-term stable microbial sampling and detection.

[0032] As described in the first embodiment above, the specific working principle is as follows: When the equipment is in normal sealed standby mode, the motor 41 is de-energized and stationary, and the movable plug 23 completely seals all water intake holes; the protective cylinder 22 physically isolates all internal sampling tubes 21 from the external water body, preventing microorganisms and impurities from directly adhering to the inner wall of the sampling tube 21. Even if a small number of microorganisms seep into the water intake hole through the sealing gap of the plug, the extended channel of the depth section 234 will significantly extend the microorganism crawling path. The narrow space has insufficient nutrient supply and wall friction resistance, preventing microorganisms from penetrating the depth section 234 to contact the sampling tube 21. This forms a double protection of plug sealing and depth section 234 antibacterial protection, preventing external bacteria from contaminating the water sample to be tested from the source.

[0033] When the periodic microbial sampling operation is started, the motor 41 is powered on and runs, driving the gear 42 to mesh with the driven gear ring 232 to rotate synchronously. The inner side of the driven gear ring 232 meshes with all the driven gears 233 synchronously, driving all the lead screws 231 to rotate synchronously in the same direction. The screw 231 thread drives the movable plug 23 to move axially in a unified manner, disengaging from the water intake hole to complete the opening.

[0034] During the movement of the movable block 23, microorganisms and impurities attached to its own surface and around the water intake hole are simultaneously peeled off, reducing the base number of miscellaneous bacteria in the water sample during the sampling period; the water intake holes are staggered in height, so the impurities and microorganisms peeled off by the block will not drift to adjacent water intake holes with the water flow, avoiding cross-interference.

[0035] At this time, water at different depths in the water storage tank 1 flows into the protective cylinder 22 through the corresponding height water intake hole and the depth section 234, and enters the independent sampling tube 21. The stratified water samples are transported separately to the external microbial detection instrument for bacterial community detection. Throughout the sampling process, the protective cylinder 22 continuously isolates the water to prevent the stratified water samples from mixing inside the cylinder, ensuring that each water sample accurately matches the true state of the microorganisms in the corresponding depth of the water.

[0036] After the sampling meets the testing requirements, the motor 41 rotates in the opposite direction, and the lead screw 231 drives the movable block 23 to reset, re-seal all water intake holes, and restore the antibacterial standby state. After sampling is completed, each sampling tube 21 can be backflushed and cleaned independently to remove residual water samples and sediments in the tube and inhibit the growth of bacterial film in the pipeline.

[0037] Throughout the entire operation process, the protective cylinder 22, the movable blocking block 23, and the deep section 234 work together to completely block the microbial contamination of the sampling tube 21, solving the industry pain points of long-term immersion sampling with contaminated water samples and distorted microbial detection. The purely mechanical transmission structure eliminates the risk of underwater electrical control failure and is suitable for long-term unattended microbial sampling and testing in various water pools.

[0038] like Figure 8-10 As shown, Example 2 is formed based on Example 1. In Example 1, the protective cylinder 22 is a fixed assembly structure, relying solely on the sealing of the movable plug 23 and the physical barrier of the depth section 234 to achieve antibacterial protection. This has two obvious defects: First, the outer wall of the protective cylinder 22 is statically immersed in water for a long time, and microorganisms and suspended impurities will continuously adhere to the surface of the cylinder and the area around the water intake hole, eventually forming a thick local bacterial film. Even if the impurities are removed by the movement of the plug, it is impossible to actively clean the microorganisms that have accumulated on the cylinder wall for a long time, continuously increasing the probability of contamination of the water sample by miscellaneous bacteria during sampling. Second, the water in the water storage tank 1 is statically stratified, and microorganisms are easily settled and gathered in local areas by gravity. The fixed protective cylinder 22 can only collect water from a very small area around the cylinder, and the concentration of microorganisms in the water sample has a local deviation, which cannot represent the overall bacterial community level of the water at that depth, reducing the representativeness of the microbial detection results.

[0039] Therefore, in this embodiment, the protective cylinder 22 is rotatably connected to the surface of the water storage tank 1. A switchable power transmission path is added to drive the protective cylinder 22 to rotate at low speed. This rotation enables active self-cleaning of the cylinder wall and slightly disturbs the uniform distribution of microorganisms in the surrounding water, thus solving the defects of fixed-point bacterial accumulation and poor representativeness of bacterial communities in the sampled water in Embodiment 1. The motor 41 drives the protective cylinder 22 to rotate around its own axis to achieve self-cleaning of the outer wall of the protective cylinder 22, reducing microbial adhesion and growth. A second drive gear 44 is provided at the bottom of the first drive gear 42. The surface is provided with a toothed groove 45, and the second drive gear 44 meshes with the toothed groove 45 to transmit the rotation of the motor 41 to the protective cylinder 22, causing the protective cylinder 22 to rotate as a whole. A clutch mechanism 43 is installed on the output shaft of the motor 41. One end of the clutch mechanism 43 is connected to the first drive gear 42, and the other end is connected to the second drive gear 44. Through the clutch action of the clutch mechanism 43, the power on and off of the first drive gear 42 and the second drive gear 44 are controlled, thereby selectively driving the movable block 23 to move to open and close the water intake hole, or driving the protective cylinder 22 to rotate as a whole to achieve self-cleaning and antibacterial.

[0040] like Figure 8-10As shown, the protective cylinder 22 is rotatably connected to the surface of the water storage tank 1. The motor 41 drives the protective cylinder 22 to rotate around its own axis to achieve self-cleaning of the outer wall of the protective cylinder 22 and reduce the adhesion and growth of microorganisms. The bottom of the drive gear 1 42 is provided with a drive gear 2 44. The surface of the protective cylinder 22 is provided with a toothed groove 45. The drive gear 2 44 meshes with the toothed groove 45 to transmit the rotation of the motor 41 to the protective cylinder 22, causing the protective cylinder 22 to rotate as a whole. The output shaft of the motor 41 is equipped with a clutch mechanism 43. One end of the clutch mechanism 43 is connected to the drive gear 1 42 and the other end is connected to the drive gear 2 44. The clutch mechanism 43 is used to control the power supply of the drive gear 1 42 and the drive gear 2 44 through the clutch action, thereby selectively driving the movable block 23 to move to open and close the water intake hole, or driving the protective cylinder 22 to rotate as a whole to achieve self-cleaning and antibacterial effect.

[0041] It should be noted that a mounting base 11 is fixedly connected to the water storage tank 1, and the protective cylinder 22 is rotatably connected to the water storage tank 1 through the mounting base 11 to achieve stable rotation of the protective cylinder 22.

[0042] It should be added that, in this embodiment, the clutch mechanism 43 is an electromagnetic jaw clutch, and the clutch input end is coaxially fixed to the output shaft of the motor 41. The clutch contains an electromagnetic coil, a driving gear, and two sets of driven gears. The two sets of driven gears are rigidly connected to the first drive gear 42 and the second drive gear 44, respectively. The control system engages / releases the driving gear by switching the current on and off of the electromagnetic coil: when one driven gear is engaged, the motor 41 only transmits power to the corresponding gear and disconnects the power on the other side; when the coil is de-energized, it is completely disengaged and the motor 41 runs under no-load.

[0043] If a mechanical shift fork clutch is selected, a small electric push rod is used to drive the shift fork to move left and right, and the active transmission plate is engaged with the two driven gears 233 respectively to realize the switching of power paths; the pneumatic clutch relies on the compression cylinder to drive engagement and disengagement. Both are mature transmission switching structures in this field.

[0044] The clutch mechanism 43 switches the power path: when engaged with drive gear 1 42 and disengaged from drive gear 2 44, only the lead screw 231 and the movable block 23 are driven to complete the stratified sampling; when engaged with drive gear 2 44 and disengaged from drive gear 1 42, the power of motor 41 is only transmitted to the protective cylinder 22, and the cylinder rotates at low speed for self-cleaning and antibacterial purposes.

[0045] It is important to note that in Example 1, the protective cylinder 22 is fixed and stationary, relying solely on the sealing of the plug, the antibacterial effect of the depth section 234, and the backflushing of the pipeline to achieve microbial protection. It cannot actively clean the microorganisms that are concentrated at specific points on the outer wall of the cylinder, resulting in uneven distribution of bacteria in the water in the sampling area. Example 2 adds a cylinder rotation function, which switches the power through the clutch mechanism 43. During non-sampling periods, the cylinder is driven to rotate at a low speed, which makes up for the shortcomings of Example 1. The antibacterial effect and the representativeness of the water sample are further improved, making it suitable for high-precision long-term microbial sampling scenarios.

[0046] As a specific working principle of Embodiment Two: After the device completes one round of stratified sampling and enters the standby antibacterial maintenance stage, the equipment control system sends a switching signal to the clutch mechanism 43. The internal transmission components of the clutch mechanism 43 complete separation and re-engagement, cutting off the power transmission of the axial drive gear 42 output by the motor 41, completely disconnecting the sampling drive branch corresponding to the lead screw 231 and the movable plug 23, and simultaneously transmitting all the output torque of the motor 41 separately to the drive gear 44 below. The drive gear 44 continuously meshes with the annular toothed groove 45 on the outer periphery of the protective cylinder 22. Relying on the stable gear pair cooperation between the toothed groove 45 and the drive gear 44, the protective cylinder 22 is driven to slowly rotate around its own central axis at a low speed and uniform speed. The speed control is smooth throughout the process, and no violent water flow impact is generated.

[0047] During the low-speed rotation of the protective cylinder 22, the outer wall of the cylinder and the end face of the water intake holes at various heights will continuously slide relative to the surrounding water. The water flow forms a continuous and gentle scouring effect, which can peel off and disperse microbial colonies, sticky bacterial films and suspended impurities that have been attached to the cylinder wall surface and the edge of the water intake holes for a long time. This physically inhibits the continuous accumulation and solidification of microorganisms in fixed areas, forming a thick bacterial film, and significantly reduces the number of miscellaneous bacteria around the water intake holes during the standby phase.

[0048] Meanwhile, the low-speed rotation of the protective cylinder 22 will only cause a weak circulation in a very small area of ​​water around the cylinder, achieving only a slight mixing and disturbance of the local water. The disturbance range is strictly limited to the area around the cylinder and will not agitate the water inside the water storage tank 1 on a large scale. Therefore, it will not destroy the original water stratification structure formed by gravity settling in the pool. The original concentration gradient of microorganisms and impurities in each water layer can be completely preserved, and there will be no large-scale mixing and cross-layering of shallow and deep water.

[0049] Before conducting microbial stratified sampling again, the cylinder can be kept in rotation for a period of time to thoroughly disperse and dilute the microorganisms and impurities accumulated on the cylinder wall and at the water intake hole into the surrounding water. Then, the power path is switched through the clutch mechanism 43, disconnecting the power of the drive gear 24 and re-engaging the drive gear 1 42 to stop the rotation of the protective cylinder 22. Subsequently, the screw 231 transmission structure is activated to drive the movable plug 23 to open the water intake hole for sampling. At this time, there are no locally high-concentration microbial clumps in the collected water. The microbial content in the water sample can accurately and uniformly represent the overall average bacterial concentration of the corresponding water layer, completely avoiding the problems of distorted microbial colony counts and species identification results and excessive detection deviations caused by microbial local deposition and enrichment.

[0050] In addition, continuous self-cleaning flushing of the cylinder wall can prevent suspended impurities from accumulating and clogging the narrow longitudinal section 234 and the water intake hole for a long time, ensuring that the water intake channel is unobstructed for a long time. It eliminates the need for frequent manual disassembly and cleaning of the protective cylinder 22, greatly reducing the workload of daily equipment maintenance. It can stably adapt to the long-term microbial sampling and testing needs of various water bodies such as underground pump room water storage tanks, water supply storage tanks, and static surface water tanks, which can be used for uninterrupted and unattended long-term microbial sampling and testing throughout the year.

[0051] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0052] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A sampling device for microbial detection, comprising a water storage tank (1), wherein a pipeline module (3) and a sampling module (2) are respectively installed on the water storage tank (1), and the sampling module (2) is connected to an external microbial detection instrument, characterized in that: The sampling module (2) includes multiple sampling tubes (21) and a protective cylinder (22). The sampling tubes (21) are located inside the protective cylinder (22) and are isolated from the water in the water storage tank (1) by the protective cylinder (22). The surface of the protective cylinder (22) is provided with water intake holes that are adapted to the number of sampling tubes (21). The multiple sampling tubes (21) are arranged to correspond to water intake holes at different heights. Each water intake hole is provided with a depth section (234) to extend the path of microorganisms crawling along the wall and reduce the invasion of microorganisms into the sampling tubes (21). The protective cylinder (22) is slidably sealed with movable plugs (23) that are adapted to the number of sampling tubes (21). The movable plugs (23) normally seal the water intake holes and move during sampling to connect the water storage tank (1) and the sampling tubes (21).

2. The sampling device for microbial detection according to claim 1, characterized in that: Each of the sampling tubes (21) is individually connected to an external microbial detection instrument. The sampling tube (21) can be adapted to the water absorption sampling mode and can also be connected to the backflushing medium to achieve backflushing cleaning, so as to avoid the accumulation of dirt and the growth of bacteria inside the sampling tube (21) that can clog the pipeline.

3. A sampling device for microbial detection according to claim 1, characterized in that: The protective cylinder (22) is fixedly connected to the water storage tank (1), and the sampling tubes (21) are all fixedly connected to the protective cylinder (22). Multiple sampling tubes (21) correspond to water intake holes at different heights, and the water intake holes are staggered on the surface of the protective cylinder (22).

4. A sampling device for microbial detection according to claim 1, characterized in that: The water storage tank (1) is equipped with a drive module (4), which is connected to the movable plug (23) for driving the movable plug (23) to move along the protective cylinder (22) to realize the opening and closing control of the water intake hole.

5. A sampling device for microbial detection according to claim 4, characterized in that: The drive module (4) includes a motor (41) fixedly connected to the water storage tank (1), and a drive gear (42) is fixedly connected to the output shaft of the motor (41).

6. A sampling device for microbial detection according to claim 5, characterized in that: The protective cylinder (22) is rotatably connected to a screw (231) that matches the number of movable plugs (23). The screw (231) is threadedly connected to the movable plugs (23). Each screw (231) is fixedly connected to a driven gear (233) at its top. A driven gear ring (232) is also rotatably connected to the protective cylinder (22). The drive gear (42) meshes with the outer side of the driven gear ring (232), and the inner side of the driven gear ring (232) meshes with the driven gear (233). Thus, the screw (231) is driven to rotate synchronously by the rotation of the motor (41), thereby driving the movable plugs (23) to move axially.

7. A sampling device for microbial detection according to claim 5, characterized in that: The protective cylinder (22) is rotatably connected to the surface of the water storage tank (1). The motor (41) drives the protective cylinder (22) to rotate around its own axis to achieve self-cleaning of the outer wall of the protective cylinder (22) and reduce the attachment and growth of microorganisms.

8. A sampling device for microbial detection according to claim 7, characterized in that: The bottom of the first drive gear (42) is provided with a second drive gear (44), and the surface of the protective cylinder (22) is provided with a tooth groove (45). The second drive gear (44) meshes with the tooth groove (45) to transmit the rotation of the motor (41) to the protective cylinder (22) and drive the protective cylinder (22) to rotate as a whole.

9. A sampling device for microbial detection according to claim 8, characterized in that: A clutch mechanism (43) is installed on the output shaft of the motor (41). One end of the clutch mechanism (43) is connected to the drive gear one (42) and the other end is connected to the drive gear two (44). The clutch mechanism (43) is used to control the power on and off of the drive gear one (42) and the drive gear two (44) through the clutch action, thereby selectively driving the movable block (23) to move to open and close the water intake hole, or driving the protective cylinder (22) to rotate as a whole to achieve self-cleaning and antibacterial.

10. A sampling device for microbial detection according to claim 7, characterized in that: The water storage tank (1) is fixedly connected to an mounting base (11), and the protective cylinder (22) is rotatably connected to the water storage tank (1) through the mounting base (11) to achieve stable rotation of the protective cylinder (22).

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