Ginkgo biloba peptide ultrasonic enzymolysis device and enzymolysis method

Abstract

The present application relates to the technical field of enzymolysis equipment, and discloses a ginkgo biloba peptide ultrasonic enzymolysis device and an enzymolysis method, which comprises an enzymolysis tank, the inside of the enzymolysis tank is sequentially provided with a top layer of partition plate and a bottom layer of partition plate along the vertical direction, the top layer of partition plate separates the upper space of the enzymolysis tank into a central enzymolysis area and flanking enzymolysis areas located on both sides of the central enzymolysis area, and the bottom layer of partition plate separates the lower space of the enzymolysis tank into a top enzymolysis area and a bottom precipitation area, the device divides the space in the tank into four functional areas, i.e., the central enzymolysis area, the flanking enzymolysis areas, the top enzymolysis area and the bottom precipitation area, by sequentially arranging the top layer of partition plate and the bottom layer of partition plate inside the enzymolysis tank along the vertical direction, so that the enzymolysis reaction of ginkgo biloba peptide, in-situ filtration and separation of precipitation are continuously completed in the same device, the whole process does not need to transfer the materials between different devices, the production cycle is shortened, and the exposure and pollution risks of the materials are reduced.

Description

Technical Field

[0001] This invention relates to the field of enzymatic hydrolysis equipment technology, specifically to an ultrasonic enzymatic hydrolysis device and method for ginkgo peptides. Background Technology

[0002] Ginkgo peptides are small-molecule bioactive peptides obtained by protease hydrolysis of ginkgo seed kernel protein. Studies have shown that ginkgo peptides possess various physiological functions, including antioxidant activity, lipid-lowering effects, and relief of alcoholic liver damage. Food-derived bioactive peptides are not only easily digested and absorbed with high safety, but also possess a wide range of physiological functions such as anti-inflammatory, immune-regulating, and blood pressure-lowering effects, making them functional factors with great development and utilization potential. Enzymatic hydrolysis is currently the main route for preparing ginkgo peptides due to its mild reaction conditions and controllable hydrolysis process.

[0003] In enzymatic hydrolysis, ultrasound-assisted technology has been introduced into the preparation of ginkgo peptides to improve hydrolysis efficiency. The cavitation effect and mechanical vibration generated by ultrasound can disrupt the plant cell wall structure, promote the release of intracellular proteins, and increase the contact area between the enzyme and the substrate, thereby improving the protein extraction rate and the rate of enzymatic hydrolysis. Therefore, ultrasound-assisted enzymatic hydrolysis has become an important technical means in the field of ginkgo peptide preparation.

[0004] However, existing ultrasonic enzymatic hydrolysis equipment still has shortcomings in practical applications. On the one hand, the structural design of traditional enzymatic hydrolysis tanks results in insufficient mixing of enzymes and substrates during the reaction, creating dead zones in the mixing process. This leads to some materials not participating uniformly in the enzymatic hydrolysis reaction, resulting in low hydrolysis efficiency and poor product uniformity. On the other hand, after the enzymatic hydrolysis reaction, the hydrolysate contains not only the target product (small molecule peptides) but also a large amount of undigested solid residues, denatured enzyme proteins, and other insoluble impurities. According to current processes, the hydrolysate needs to be transferred from the enzymatic hydrolysis tank and then sent to a centrifuge for solid-liquid separation. This step-by-step operation has significant drawbacks: the material transfer process increases the number of steps and labor intensity, and also increases the risk of material exposure and contamination; solid residues in the hydrolysate directly entering the centrifuge can easily cause blockage and wear of the centrifuge equipment, affecting separation efficiency and service life; in addition, the hydrolysate may undergo over-enzymatic hydrolysis or product degradation during transfer and waiting for centrifugation, affecting the final product quality. These problems restrict further improvements in the production efficiency and product quality of ginkgo peptides. Summary of the Invention

[0005] The purpose of this invention is to provide an ultrasonic enzymatic hydrolysis device and method for Ginkgo peptides to solve the problems mentioned in the background art.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a ginkgo peptide ultrasonic enzymatic hydrolysis device, comprising:

[0007] An enzymatic hydrolysis vessel has a top partition plate and a bottom partition plate arranged vertically inside. The top partition plate divides the upper space of the enzymatic hydrolysis vessel into a central enzymatic hydrolysis zone and side enzymatic hydrolysis zones located on both sides. The bottom partition plate divides the lower space of the enzymatic hydrolysis vessel into a top enzymatic hydrolysis zone and a bottom sedimentation zone.

[0008] The top and bottom ends of the top partition plate are respectively provided with transverse flow holes to connect the central enzymatic hydrolysis zone and the side enzymatic hydrolysis zone to form an enzymatic hydrolysis circulation path.

[0009] Adjustable filter plates are embedded in both sides of the bottom partition plate, and longitudinal flow holes are opened through the bottom partition plate on the upper and lower sides corresponding to the adjustable filter plates to guide the enzymatically hydrolyzed liquid phase to the bottom sedimentation area.

[0010] The first stirring mechanism is located in the top enzymatic hydrolysis zone and is used to drive the material to circulate and be stirred by enzymatic hydrolysis.

[0011] The second stirring mechanism is located in the bottom sedimentation zone and is used to stir the sediment and assist in slag discharge.

[0012] An ultrasonic generator is fixedly installed on the side wall of the enzymatic hydrolysis vessel to assist the enzymatic hydrolysis reaction;

[0013] The enzymatic hydrolysis reaction monitoring mechanism includes a temperature sensor, a pH sensor, and a real-time turbidity sensor integrated and installed on the enzymatic hydrolysis tank. The real-time turbidity sensor is used to monitor the enzymatic hydrolysis process and control the opening timing of the adjustable filter plate.

[0014] According to the above technical solution, the enzymatic hydrolysis vessel includes:

[0015] The tank body is composed of a top cover, a body, and a bottom cover arranged in sequence, and the side wall of the body is embedded with a spirally wound heat exchange tube.

[0016] Support legs are evenly distributed in a circle on the bottom of the side wall of the tank body, and shock-absorbing supports are fixedly installed at the ends of each support leg.

[0017] The feed inlet is located at the top of the tank cover;

[0018] The discharge port is located on the side wall of the tank body;

[0019] The first slag discharge port is provided on the side wall of the tank body, and the inner end of the first slag discharge port extends to the bottom of the top enzymatic hydrolysis zone;

[0020] The second slag discharge port is located at the lower edge of the tank bottom cover.

[0021] According to the above technical solution, the first stirring mechanism includes:

[0022] The central positioning shaft is movably located at the center of the tank body, with its top penetrating through and movably installed on the tank top cover, and its bottom movably installed on the bottom partition plate;

[0023] The first spiral propulsion plate is fixedly sleeved on the central positioning shaft;

[0024] The side driven shafts are arranged in a mirror image symmetrically on both sides of the central positioning shaft. The top of each side driven shaft passes through and is movably installed on the top cover of the tank, and its bottom is movably installed on the bottom partition plate.

[0025] The second spiral propulsion plate is fixedly sleeved on each of the side driven shafts;

[0026] The first stirring group is respectively set at the upper and lower ends of each of the second spiral propulsion plates. Each first stirring group consists of first stirring rods evenly distributed circumferentially on the corresponding side driven shaft.

[0027] A power drive unit is located at the upper end of the enzymatic hydrolysis vessel and is used to drive the central positioning shaft and the side driven shaft to rotate.

[0028] The first stirring rod has a non-circular cross-section, and each of the first stirring rods is arranged at an angle relative to the horizontal plane.

[0029] In the same first stirring group, the adjacent first stirring rods are of different lengths and are arranged in an alternating pattern of long and short.

[0030] According to the above technical solution, the power drive group includes:

[0031] A fixing bracket is fixedly installed at the upper end of the enzymatic hydrolysis vessel;

[0032] The first servo motor is fixedly installed at the lower end of the fixed frame, and a first pulley is fixedly installed at its output end. The bottom of the first pulley is fixedly connected to the top of the central positioning shaft.

[0033] The second pulley is fixedly installed on the top of each of the side driven shafts;

[0034] A drive belt is used to drive the first pulley and each of the second pulleys;

[0035] Angle-increasing pulleys are respectively disposed between the first pulley and each of the second pulleys, and the angle-increasing pulleys overlap the outer side wall of the transmission belt to increase the wrap angle between the transmission belt and the corresponding pulley.

[0036] According to the above technical solution, the second stirring mechanism includes:

[0037] The second servo motor is fixedly installed at the lower end of the enzymatic hydrolysis vessel;

[0038] A bottom rotating shaft is movably installed in the bottom sedimentation area, and the bottom end of the bottom rotating shaft is fixedly connected to the output end of the second servo motor.

[0039] The second stirring assembly consists of second stirring rods that are circumferentially distributed on the bottom rotating shaft;

[0040] The free end of the second stirring rod is provided with an expanding stirring head, the outer diameter of which is larger than the diameter of the second stirring rod.

[0041] According to the above technical solution, the bottom partition plate is composed of a central bottom plate and side wing inclined plates symmetrically arranged on both sides of the central bottom plate;

[0042] The end of the central positioning shaft is movably mounted on the upper end of the central base plate;

[0043] Each of the aforementioned side wing inclined plates is inclined downward toward the corresponding tank side wall, and the longitudinal flow holes are evenly distributed on the side wing inclined plates;

[0044] The ends of each of the side driven shafts are movably mounted on the corresponding side wing inclined plates, and the longitudinal flow holes are located on both sides of each of the side driven shafts.

[0045] According to the above technical solution, the adjustable filter plate includes:

[0046] The movable filter screen is slidably installed in a sliding groove inside the inclined plate of the side wing;

[0047] A reset spring, one end of which is fixedly connected to the side wall of the sliding groove, and the other end of which is fixedly connected to the side wall of the movable filter screen;

[0048] A torque drive shaft is movably mounted at the bottom center of the central base plate. The bottom end of the torque drive shaft is fixedly connected to the bottom rotating shaft via an electromagnetic clutch. A torque head is fixedly mounted at the top end of the torque drive shaft. An angle locking button is provided at the top of the torque head to rotate and engage with the top of the central base plate.

[0049] A flexible connecting cable, one end of which is fixedly installed to one end of the movable filter screen, and the other end of which is fixedly installed to the torque head;

[0050] The movable filter screen consists of a filter screen frame and a detachable filter screen inserted into one side of the filter screen frame;

[0051] A replacement port corresponding to the movable filter screen is provided through the side wall of the tank body, and a sealing plate is detachably fixed to the replacement port by bolts.

[0052] According to the above technical solution, the transverse flow hole is configured as a long groove, and the interior of the top partition plate is provided with an adjustment mechanism corresponding to each transverse flow hole;

[0053] The adjustment mechanism includes:

[0054] The baffle plate is movably installed in the lifting groove opened inside the side wall of the top partition plate, and the baffle plate is provided with alignment holes that correspond one-to-one with each of the transverse flow holes.

[0055] Compression springs are evenly distributed at the upper end of the baffle plate, and the compression springs are in a compressed state, and their elastic force always pushes the baffle plate to move upward.

[0056] A triangular sliding bracket is fixedly installed on the baffle plate;

[0057] An electric telescopic rod is fixedly installed in the middle of the top partition plate. An extrusion plate is fixedly installed at the telescopic end of the electric telescopic rod. The ends of the extrusion plates slide against the inclined surfaces of the corresponding triangular sliding frames.

[0058] When the electric telescopic rod extends, the squeezing plate pushes the triangular sliding frame and the baffle plate downward, causing the alignment hole and the transverse flow hole to be misaligned, thereby changing the actual flow area of ​​the transverse flow hole.

[0059] According to the above technical solution, an outer edge guide block is inlaid and installed on the upper edge of the alignment hole, and a lower protrusion is provided at the lower center of the outer edge guide block.

[0060] A bottom blocking block is inlaid at the bottom edge of the transverse flow hole, and an upper protrusion is provided at the center of the upper end of the bottom blocking block;

[0061] A flow channel is formed between the outer edge guide block and the bottom shield block, allowing fluid to pass through. The flow channel exhibits a trend of first contracting and then expanding along the fluid direction.

[0062] An enzymatic hydrolysis method for ginkgo peptides using an ultrasonic enzymatic hydrolysis device includes the following steps:

[0063] S1: Add ginkgo protein raw material and water into the top enzymatic hydrolysis zone of the enzymatic hydrolysis tank in proportion, start the first stirring mechanism to stir and mix, and monitor and adjust the temperature and pH value of the enzymatic hydrolysis system to a preset range through the temperature sensor and the pH sensor.

[0064] S2: Add protease to the top enzymatic hydrolysis zone, start the ultrasonic generator to apply ultrasonic waves, and under the drive of the first stirring mechanism, the material in the top enzymatic hydrolysis zone circulates between the central enzymatic hydrolysis zone and the side enzymatic hydrolysis zone through the transverse flow hole at the upper end of the top partition plate to carry out ultrasonic-assisted enzymatic hydrolysis reaction.

[0065] S3: The turbidity change of the enzymatic hydrolysis system is monitored in real time by the real-time turbidity sensor. When the turbidity reaches the preset threshold, the enzymatic hydrolysis reaction is determined to have reached the endpoint.

[0066] S4: Open the adjustable filter plates on both sides of the bottom partition plate, so that the enzymatic hydrolysate in the top enzymatic hydrolysis zone is filtered by the adjustable filter plates and enters the bottom sedimentation zone through the longitudinal flow hole, and the solid residue is trapped in the top enzymatic hydrolysis zone.

[0067] S5: Start the second stirring mechanism to stir the enzymatic hydrolysate that has entered the bottom sedimentation zone, so that the precipitate settles in the bottom sedimentation zone and is discharged through the second slag discharge port. Collect the supernatant in the bottom sedimentation zone to obtain the ginkgo peptide enzymatic hydrolysate.

[0068] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0069] (1) The three-stage process of enzymatic hydrolysis, filtration and precipitation is integrated to achieve continuous operation.

[0070] This equipment divides the interior of the enzymatic hydrolysis tank into four functional zones: a central enzymatic hydrolysis zone, a side enzymatic hydrolysis zone, a top enzymatic hydrolysis zone, and a bottom sedimentation zone, using a top and bottom partition plate arranged vertically. This allows the three core processes of ginkgo peptide enzymatic hydrolysis, in-situ filtration, and sedimentation separation to be completed continuously within the same equipment. Horizontal flow holes at the top and bottom of the top partition plate, in conjunction with a first stirring mechanism, create a circulating flow path between the central and side enzymatic hydrolysis zones, enabling efficient enzymatic hydrolysis through dynamic circulation. Adjustable filter plates embedded on both sides of the bottom partition plate, along with longitudinal flow holes, immediately achieve in-situ separation of the hydrolysate from solid residue after enzymatic hydrolysis. The bottom sedimentation zone utilizes a second stirring mechanism to assist in the settling and discharge of sediment. The entire process eliminates the need to transfer materials between different devices, shortening the production cycle and reducing material exposure and contamination risks.

[0071] (2) The synergistic effect of the circulating flow path and ultrasound improves the efficiency and uniformity of enzymatic hydrolysis.

[0072] In the first stirring mechanism, the central positioning shaft drives the first helical propeller, and the side driven shaft drives the second helical propeller and the first stirring rod. The first and second helical propellers are set in opposite directions, forming a forced circulation flow path of "central output, side reception and return"—the first helical propeller in the central enzymatic hydrolysis zone pushes the material upward, flows into the side enzymatic hydrolysis zone through the transverse flow hole at the top, is pushed downward with the assistance of the second helical propeller, and then flows back to the central enzymatic hydrolysis zone through the transverse flow hole at the bottom. This circulation flow path allows all the material to repeatedly pass through the ultrasonic action area of ​​the ultrasonic generator. The ultrasonic cavitation effect continuously disrupts the cell wall structure of Ginkgo biloba, promoting the release of intracellular proteins. At the same time, the spatiotemporal synergy of the flow field and the sound field effectively eliminates reaction dead zones, ensuring uniform contact between the enzyme and the substrate throughout the entire tank, improving the efficiency of the enzymatic hydrolysis reaction and the uniformity of the products.

[0073] (3) The dual optimization design of the circulation channel realizes controllable circulation flow and enhanced local mixing.

[0074] The flow channel at the transverse flow orifice features a dual optimized design of "adjustable opening" and "contraction followed by expansion." The adjustable opening function is achieved through an adjustment mechanism—an electric telescopic rod drives the baffle plate to move, causing a relative misalignment between the alignment orifice and the transverse flow orifice. This allows for dynamic adjustment of the flow area based on different flow requirements in the initial, middle, and later stages of enzymatic hydrolysis. Simultaneously, it links with a real-time turbidity sensor to achieve intelligent closed-loop control of "turbidity sensing → opening adjustment → flow rate change." The venturi configuration of contraction followed by expansion accelerates the fluid, generating strong turbulence to enhance micro-mixing, and then decelerates to form a vortex zone to eliminate dead zones. Local cavitation effects are also generated in the contraction section, which, combined with the ultrasonic action of the ultrasonic generator, creates cavitation superposition, resulting in a more uniform cavitation effect distribution throughout the entire top enzymatic hydrolysis zone. This overcomes the energy attenuation and uneven distribution problems of a single ultrasonic source.

[0075] (4) The adjustable filter plate and the longitudinal flow hole are linked for precise solid-liquid separation.

[0076] The adjustable filter plates embedded on both sides of the bottom partition plate consist of a precise linkage system comprised of a movable filter screen, a return spring, a torque drive shaft, an electromagnetic clutch, a torque head, a flexible connecting cable, and an angle locking button. The longitudinal flow holes are normally closed, corresponding to the closed end of the filter screen holder. When filtration is required, the electromagnetic clutch engages, the bottom rotating shaft drives the torque drive shaft to rotate, the return spring contracts, pulling the movable filter screen to slide and align the detachable filter screen with the longitudinal flow holes. After the electromagnetic clutch disengages, the angle locking button maintains this state, ensuring stable opening of the filtration channel. After filtration, the reverse operation restores the closure of the longitudinal flow holes. This design achieves precise on / off control of the longitudinal flow holes, ensuring complete isolation of the bottom sedimentation zone during the enzymatic hydrolysis stage and smooth liquid phase flow during the filtration stage.

[0077] (5) Differentiated design of dual-zone stirring to optimize process effect

[0078] The first and second stirring mechanisms are designed differently to meet the functional requirements of the top enzymatic hydrolysis zone and the bottom sedimentation zone, respectively. The first stirring rod in the top enzymatic hydrolysis zone has a non-circular cross-section and is arranged at an angle relative to the horizontal plane. Adjacent stirring rods in the same first stirring group have unequal lengths and are arranged in an alternating pattern, generating multi-directional disturbance forces to eliminate dead zones in different radius areas and meet the high-efficiency mixing requirements of the enzymatic hydrolysis reaction. The second stirring rod in the bottom sedimentation zone has an expanded-diameter stirring head at its free end, with an outer diameter larger than the rod's body diameter. This increases the disturbance range at the end, effectively eliminating dead zones at the edge of the elliptical tank bottom, preventing large-area caking of sediment at the bottom, and pushing the sediment towards the second discharge port, thus meeting the sedimentation zone's requirements for gentle stirring and auxiliary discharge. Attached Figure Description

[0079] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0080] Figure 1 This is a first perspective view of the present invention;

[0081] Figure 2 This is a second perspective view of the present invention;

[0082] Figure 3 This is a third perspective view of the present invention;

[0083] Figure 4 This is a first partial three-dimensional schematic diagram of the present invention;

[0084] Figure 5 This is a second partial perspective view of the present invention;

[0085] Figure 6 This is a third partial perspective view of the present invention;

[0086] Figure 7 This is a fourth partial perspective view of the present invention;

[0087] Figure 8 This is a fifth partial perspective view of the present invention;

[0088] Figure 9 This is a sixth partial perspective view of the present invention;

[0089] Figure 10 This is a third-dimensional schematic diagram of the seventh part of the present invention;

[0090] Figure 11 This is the eighth partial perspective view of the present invention;

[0091] Figure 12 This is a third-dimensional schematic diagram of the ninth part of the present invention;

[0092] In the diagram: 1-Enzymatic hydrolysis tank, 11-Tank body, 111-Tank top cover, 112-Tank body, 113-Tank bottom cover, 12-Support leg, 121-Shock-absorbing support, 13-Inlet, 14-Outlet, 15-First slag discharge port, 16-Second slag discharge port, 2-Top layer partition plate, 21-Transverse flow hole, 22-Adjusting mechanism, 221-Baffle plate, 222-Lifting groove, 223-Alignment hole, 2231-Outer edge guide block, 2232- 2233 - Bottom baffle block; 2234 - Upper protrusion; 2235 - Flow channel; 224 - Compression spring; 225 - Triangular sliding bracket; 226 - Electric telescopic rod; 227 - Squeezing plate; 3 - Bottom partition plate; 31 - Adjustable filter plate; 311 - Movable filter screen; 3111 - Filter screen frame; 3112 - Removable filter screen; 312 - Sliding groove; 313 - Return spring; 314 - Torque drive shaft; 315 - Electromagnetic... Clutch, 316 Torque Head, 317 Flexible Connecting Cable, 32 Longitudinal Flow Hole, 33 Central Base Plate, 34 Side Wing Inclined Plate, 35 Replacement Port, 351 Sealing Plate, 4 Ultrasonic Generator, 5 First Stirring Mechanism, 51 Central Positioning Shaft, 52 First Spiral Propeller, 53 Side Driven Shaft, 54 Second Spiral Propeller, 55 First Stirring Group, 551 First Stirring Rod, 56 Power Drive Group, 561 Fixed Frame, 562 First Servo Motor, 563 First Pulley, 564 Second Pulley, 565 Transmission Belt, 566 Angle-Increasing Wheel, 6 Second Stirring Mechanism, 61 Second Servo Motor, 62 Bottom Rotating Shaft, 63 Second Stirring Group, 631 Second Stirring Rod, 632 Expanding Diameter Stirring Head, 7 Enzymatic Reaction Monitoring Mechanism, 71 Temperature Sensor, 72 pH Sensor, 73 Real-time Turbidity Sensor. Detailed Implementation

[0093] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0094] Please see Figure 1-12 The present invention provides a technical solution: a ginkgo peptide ultrasonic enzymatic hydrolysis device, comprising:

[0095] The enzymatic hydrolysis tank 1 has a top partition plate 2 and a bottom partition plate 3 arranged vertically inside. The top partition plate 2 divides the upper space of the enzymatic hydrolysis tank 1 into a central enzymatic hydrolysis zone and side enzymatic hydrolysis zones located on both sides. The bottom partition plate 3 divides the lower space of the enzymatic hydrolysis tank 1 into a top enzymatic hydrolysis zone and a bottom sedimentation zone.

[0096] The top partition plate 2 is provided with transverse flow holes 21 at its upper and lower ends to connect the central enzymatic hydrolysis zone and the side enzymatic hydrolysis zone to form an enzymatic hydrolysis circulation path.

[0097] Adjustable filter plates 31 are embedded in both sides of the bottom partition plate 3, and longitudinal flow holes 32 are opened through the bottom partition plate 3 on the upper and lower sides corresponding to the adjustable filter plates 31, for guiding the enzymatically hydrolyzed liquid phase to the bottom sedimentation area.

[0098] The first stirring mechanism 5 is disposed in the top enzymatic hydrolysis zone and is used to drive the material to circulate and enzymatically hydrolyze.

[0099] The second stirring mechanism 6 is located in the bottom sedimentation zone and is used to stir the sediment and assist in slag discharge.

[0100] An ultrasonic generator 4 is fixedly installed on the side wall of the enzymatic hydrolysis vessel 1 to assist the enzymatic hydrolysis reaction.

[0101] The enzymatic hydrolysis reaction monitoring mechanism 7 includes a temperature sensor 71, a pH sensor 72, and a real-time turbidity sensor 73 integrated on the enzymatic hydrolysis tank 1. The real-time turbidity sensor 73 is used to monitor the enzymatic hydrolysis process and control the opening timing of the adjustable filter plate 31.

[0102] Specifically, the enzymatic hydrolysis tank 1 includes:

[0103] The tank body 11 is composed of a tank top cover 111, a tank body 112 and a tank bottom cover 113 arranged in sequence. The side wall of the tank body 112 is embedded with a spirally wound heat exchange tube.

[0104] Support legs 12 are evenly distributed in a circle on the bottom of the side wall of the tank body 112, and shock-absorbing supports 121 are fixedly installed at the end of each support leg 12.

[0105] The feed inlet 13 is located on the top of the tank cover 111;

[0106] The discharge port 14 is located on the side wall of the tank body 112;

[0107] The first slag discharge port 15 is provided on the side wall of the tank body 112, and the inner end of the first slag discharge port 15 extends to the bottom of the top enzymatic hydrolysis zone.

[0108] The second slag discharge port 16 is located at the lower edge of the tank bottom cover 113;

[0109] Specifically, the first stirring mechanism 5 includes:

[0110] The central positioning shaft 51 is movably set in the center of the tank body 11, with its top penetrating through and movably installed on the tank top cover 111, and its bottom movably installed on the bottom partition plate 3;

[0111] The first spiral propulsion plate 52 is fixedly sleeved on the central positioning shaft 51;

[0112] The side driven shafts 53 are mirror-symmetrically arranged on both sides of the central positioning shaft 51. The top of each side driven shaft 53 passes through and is movably installed on the tank top cover 111, and its bottom is movably installed on the bottom partition plate 3.

[0113] The second spiral propulsion plate 54 is fixedly sleeved on each of the side driven shafts 53;

[0114] The first stirring group 55 is respectively disposed at the upper end and the lower end of each of the second spiral propulsion plates 54. Each first stirring group 55 is composed of first stirring rods 551 that are circumferentially distributed on the corresponding side driven shaft 53.

[0115] A power drive assembly 56 is located at the upper end of the enzymatic hydrolysis tank 1 and is used to drive the central positioning shaft 51 and the side driven shaft 53 to rotate.

[0116] The first stirring rod 551 has a non-circular cross-section, and each of the first stirring rods 551 is arranged at an angle relative to the horizontal plane.

[0117] In the same first stirring group 55, the adjacent first stirring rods 551 are of different lengths and are arranged in an alternating pattern of long and short.

[0118] Specifically, the power drive assembly 56 includes:

[0119] The fixing bracket 561 is fixedly installed on the upper end of the enzymatic hydrolysis tank 1;

[0120] The first servo motor 562 is fixedly installed at the lower end of the fixed frame 561, and the output end of the first pulley 563 is fixedly installed. The bottom of the first pulley 563 is fixedly connected to the top of the central positioning shaft 51.

[0121] The second pulley 564 is fixedly installed on the top of each of the side driven shafts 53;

[0122] A drive belt 565 is connected between the first pulley 563 and each of the second pulleys 564.

[0123] Angle-increasing pulleys 566 are respectively disposed between the first pulley 563 and each of the second pulleys 564, and the angle-increasing pulleys 566 overlap the outer side wall of the transmission belt 565 to increase the wrap angle between the transmission belt 565 and the corresponding pulley.

[0124] Specifically, the second stirring mechanism 6 includes:

[0125] The second servo motor 61 is fixedly installed at the lower end of the enzymatic hydrolysis tank 1;

[0126] The bottom rotating shaft 62 is movably installed in the bottom sedimentation area, and the bottom end of the bottom rotating shaft 62 is fixedly connected to the output end of the second servo motor 61.

[0127] The second stirring group 63 is composed of second stirring rods 631 that are circumferentially distributed on the bottom rotating shaft 62;

[0128] The free end of the second stirring rod 631 is provided with an expanded diameter stirring head 632, the outer diameter of which is larger than the diameter of the second stirring rod 631.

[0129] Specifically, the bottom partition plate 3 is composed of a central bottom plate 33 and side wing inclined plates 34 symmetrically arranged on both sides of the central bottom plate 33;

[0130] The end of the central positioning shaft 51 is movably mounted on the upper end of the central base plate 33;

[0131] Each of the side wing inclined plates 34 is inclined downward toward the corresponding side wall of the tank body 112, and the longitudinal flow holes 32 are evenly distributed on the side wing inclined plates 34;

[0132] The ends of each of the side driven shafts 53 are movably mounted on the corresponding side wing inclined plates 34, and the longitudinal flow holes 32 are respectively located on both sides of each of the side driven shafts 53;

[0133] Specifically, the adjustable filter plate 31 includes:

[0134] The movable filter screen 311 is slidably installed in the sliding groove 312 opened inside the side wing inclined plate 34;

[0135] The return spring 313 has one end fixedly connected to the side wall of the sliding groove 312 and the other end fixedly connected to the side wall of the movable filter screen 311.

[0136] Torque drive shaft 314 is movably installed at the bottom center of the central base plate 33. The bottom end of the torque drive shaft 314 is fixedly connected to the bottom rotating shaft 62 through an electromagnetic clutch 315. A torque head 316 is fixedly installed at the top end of the torque drive shaft 314. The top of the torque head 316 is provided with an angle locking button 317 that rotates and engages with the top of the central base plate 33.

[0137] A flexible connecting cable 317 has one end fixedly installed on one end of the movable filter screen 311, and the other end fixedly installed on the torque head 316;

[0138] The movable filter screen 311 consists of a filter screen frame 3111 and a detachable filter screen 3112 inserted into one side of the filter screen frame 3111;

[0139] A replacement port 35, corresponding to the movable filter screen 311, is provided through the side wall of the tank body 112. A sealing plate 351 is detachably fixed to the replacement port 35 by bolts 352.

[0140] Specifically, the transverse flow hole 21 is configured as an elongated groove, and the interior of the top partition plate 2 is provided with an adjustment mechanism 22 corresponding to each transverse flow hole 21;

[0141] The adjustment mechanism 22 includes:

[0142] The baffle plate 221 is movably installed in the lifting groove 222 opened inside the side wall of the top partition plate 2. The baffle plate 221 is provided with alignment holes 223 that correspond one-to-one with each of the transverse flow holes 21.

[0143] Compression springs 224 are evenly distributed on the upper end of the baffle plate 221, and the compression springs 224 are in a compressed state, and their elastic force always pushes the baffle plate 221 to move upward.

[0144] The triangular sliding bracket 225 is fixedly installed on the baffle plate 221;

[0145] An electric telescopic rod 226 is fixedly installed in the middle of the top partition plate 2. An extrusion plate 227 is fixedly installed at the telescopic end of the electric telescopic rod 226. The ends of the extrusion plates 227 slide against the inclined surfaces of the corresponding triangular sliding frames 225.

[0146] When the electric telescopic rod 226 extends, the squeezing plate 227 pushes the triangular sliding frame 225 and the baffle plate 221 to move downward, so that the alignment hole 223 and the transverse flow hole 21 are relatively misaligned, thereby changing the actual flow area of ​​the transverse flow hole 21.

[0147] Specifically, an outer edge guide block 2231 is inlaid and installed on the upper edge of the alignment hole 223, and a lower protrusion 2232 is provided at the lower center of the outer edge guide block 2231.

[0148] A bottom blocking block 2233 is inlaid and installed at the bottom edge of the transverse flow hole 21, and an upper protrusion 2234 is provided at the upper center of the bottom blocking block 2233.

[0149] A flow channel 2235 is formed between the outer edge guide block 2231 and the bottom shield block 2233, allowing fluid to pass through. The flow channel 2235 exhibits a trend of first contraction and then expansion along the fluid direction.

[0150] An enzymatic hydrolysis method for ginkgo peptides using an ultrasonic enzymatic hydrolysis device includes the following steps:

[0151] S1: Add ginkgo protein raw material and water into the top enzymatic hydrolysis zone of the enzymatic hydrolysis tank 1 in proportion, start the first stirring mechanism 5 to stir and mix, and monitor and adjust the temperature and pH value of the enzymatic hydrolysis system to the preset range through the temperature sensor 71 and the pH sensor 72.

[0152] S2: Add protease to the top enzymatic hydrolysis zone, start the ultrasonic generator 4 to apply ultrasonic waves, and under the drive of the first stirring mechanism 5, the material in the top enzymatic hydrolysis zone circulates between the central enzymatic hydrolysis zone and the side enzymatic hydrolysis zone through the transverse flow hole 21 at the upper end of the top partition plate 2 to carry out ultrasonic-assisted enzymatic hydrolysis reaction.

[0153] S3: The turbidity change of the enzymatic hydrolysis system is monitored in real time by the real-time turbidity sensor 73. When the turbidity reaches the preset threshold, the enzymatic hydrolysis reaction is determined to have reached the endpoint.

[0154] S4: Open the adjustable filter plates 31 on both sides of the bottom partition plate 3, so that the enzymatic hydrolysate in the top enzymatic hydrolysis zone is filtered by the adjustable filter plates 31 and enters the bottom sedimentation zone through the longitudinal flow hole 32, and the solid residue is trapped in the top enzymatic hydrolysis zone.

[0155] S5: Start the second stirring mechanism 6 to stir the enzymatic hydrolysate that has entered the bottom sedimentation zone, so that the precipitate settles in the bottom sedimentation zone and is discharged through the second slag discharge port 16. Collect the supernatant in the bottom sedimentation zone to obtain the ginkgo peptide enzymatic hydrolysate.

[0156] This ginkgo peptide ultrasonic enzymatic hydrolysis equipment is an integrated bioreactor that combines enzymatic hydrolysis, in-situ filtration, and precipitation separation. The core design concept of the equipment is to divide the internal space of the enzymatic hydrolysis tank 1 into three interconnected reaction zones with distinct functions through a top-level partition plate 2 and a bottom-level partition plate 3, achieving continuous operation of "enzymatic hydrolysis-filtration-precipitation". Simultaneously, the ultrasonic assistance of the ultrasonic generator 4 and the intelligent monitoring of the enzymatic hydrolysis reaction monitoring mechanism 7 enable efficient and controllable ginkgo peptide enzymatic hydrolysis production.

[0157] In this equipment, the inlet 13 and outlet 14 of the enzymatic hydrolysis tank 1 can be configured in multiple ways, not limited to a specific location, and can also be configured on the side wall of the enzymatic hydrolysis tank 1. The positions of the temperature sensor 71, pH sensor 72, and real-time turbidity sensor 73 are also not limited, with the temperature sensor 71 arranged in a top-middle-bottom distributed configuration. These are all common technical methods in the prior art, and can be adaptively configured; therefore, they will not be elaborated upon further here.

[0158] The detailed operating procedure of this Ginkgo peptide ultrasonic enzymatic hydrolysis equipment is as follows:

[0159] Step S1: Feeding and Mixing

[0160] Ginkgo protein raw material and water are added to the top enzymatic hydrolysis zone of enzymatic hydrolysis tank 1 in a certain proportion. Before ultrasonic enzymatic hydrolysis, the central enzymatic hydrolysis zone and the side enzymatic hydrolysis zones located on both sides can be isolated to separate the raw materials. The first stirring mechanism 5 is started to stir and mix. In the first stirring mechanism 5, the central positioning shaft 51 drives the first spiral propeller 52 to rotate, while the side driven shafts 53, which are mirror-symmetrically arranged on both sides of the central positioning shaft 51, drive the second spiral propeller 54 and the first stirring rod 551 to rotate, together to complete the uniform dispersion and mixing of materials. The first stirring rod 551 has a non-circular cross-section, and each first stirring rod 551 is arranged at an angle relative to the horizontal plane, which can generate multi-directional disturbance force; in the same first stirring group 55, the adjacent first stirring rods 551 have different lengths and are arranged in an alternating pattern, which can eliminate the flow dead zone in different radius areas and achieve uniform mixing of materials.

[0161] The temperature and pH of the enzymatic hydrolysis system are monitored by temperature sensor 71 and pH sensor 72, and the temperature is adjusted by the spirally wound heat exchange tube embedded in the side wall of tank body 112 until the system temperature and pH reach the preset enzymatic hydrolysis reaction conditions.

[0162] Step S2: Ultrasound-assisted enzymatic hydrolysis reaction

[0163] Add protease to the top enzymatic hydrolysis zone and activate the ultrasonic generator 4 to apply ultrasound. The ultrasonic generator 4 is fixedly installed on the side wall of the enzymatic hydrolysis vessel 1. Applying ultrasound to the top enzymatic hydrolysis zone, the microjets and shock waves generated by the cavitation effect disrupt the cell wall structure of Ginkgo biloba, promote the release of intracellular proteins, and increase the contact area between the enzyme and the substrate.

[0164] During ultrasonic enzymatic hydrolysis, the central enzymatic hydrolysis zone and the flank enzymatic hydrolysis zones on either side circulate. Driven by the first stirring mechanism 5, the material in the top enzymatic hydrolysis zone circulates between the central and flank enzymatic hydrolysis zones through the transverse flow holes 21 at the upper end of the top partition plate 2, forming a circulation path. The top partition plate 2 has transverse flow holes 21 at its upper and lower ends to connect the central and flank enzymatic hydrolysis zones.

[0165] The first spiral propeller plate 52 and the second spiral propeller plate 54 are arranged in opposite directions, forming a cycle with output in the middle and reception and return on both sides. The circulation direction preferentially selects the first spiral propeller plate 52 in the central enzymatic hydrolysis zone to push upward, flow into the side enzymatic hydrolysis zone through the top flow channel 2235, and is pushed downward by the second spiral propeller plate 54, flowing back to the central enzymatic hydrolysis zone through the bottom flow channel 2235.

[0166] During this process, the actual flow area of ​​the transverse flow hole 21 can be dynamically adjusted by the adjustment mechanism 22, and the opening degree of the flow channel 2235 can be adaptively adjusted according to the concentration. The transverse flow hole 21 is set as a long groove, and the top partition plate 2 is equipped with an adjustment mechanism 22 corresponding to each transverse flow hole 21. When the electric telescopic rod 226 of the adjustment mechanism 22 extends, it pushes the triangular sliding frame 225 and the baffle plate 221 downward through the squeezing plate 227, so that the alignment holes 223 on the baffle plate 221 corresponding to each transverse flow hole 21 are relatively misaligned with the transverse flow hole 21, thereby changing the actual flow area of ​​the transverse flow hole 21; the compression springs 224 are evenly distributed on the upper end of the baffle plate 221 and are in a compressed state. Their elasticity always pushes the baffle plate 221 upward, and the baffle plate 221 is reset when the electric telescopic rod 226 retracts. Meanwhile, an outer edge guide block 2231 is embedded in the upper edge of the alignment hole 223, and a lower protrusion 2232 is provided at the lower center of the outer edge guide block 2231; a bottom blocking block 2233 is embedded in the bottom edge of the transverse flow hole 21, and an upper protrusion 2234 is provided at the upper center of the bottom blocking block 2233; a flow channel 2235 is formed between the outer edge guide block 2231 and the bottom blocking block 2233, allowing fluid to pass through. The flow channel 2235 exhibits a trend of first contraction and then expansion along the fluid direction, causing the fluid to generate a Venturi effect when passing through—first accelerating and then decelerating, enhancing the local turbulence and mixing effect. When the fluid flows from the central enzymatic hydrolysis zone to the lateral enzymatic hydrolysis zone through the flow channel 2235: in the contraction section, the channel cross-section gradually narrows, the fluid is accelerated, the flow velocity increases sharply, and strong turbulent disturbances are generated. This turbulence helps to break up any potentially high-concentration local areas, allowing for more thorough contact between the enzyme and substrate at the microscale. In the expansion section, the fluid enters after passing through the narrowest cross-section, where the velocity drops sharply, creating vortex and recirculation zones behind the channel outlet, further enhancing the local mixing effect. In the contraction section, the surge in velocity leads to a decrease in local pressure, causing dissolved gases to precipitate and form microbubbles. Upon entering the expansion section and regaining pressure, these bubbles instantly collapse, generating a local cavitation effect. This effect, combined with the ultrasonic action of the ultrasonic generator 4, breaks down residual cell walls and creates a superimposed enhancement of ultrasonic-Venturi cavitation, resulting in a more uniform distribution of cavitation throughout the entire top enzymatic hydrolysis zone, overcoming the potential energy attenuation and uneven distribution problems associated with a single ultrasonic source.

[0167] Temperature sensor 71 and pH sensor 72 continuously monitor the reaction conditions, and the system temperature is dynamically controlled by the heat exchange tube to ensure that the enzymatic hydrolysis reaction proceeds stably under optimal conditions until the enzymatic hydrolysis reaction is completed.

[0168] The opening degree of the flow channel 2235 is dynamically adjusted by the adjustment mechanism 22. The electric telescopic rod 226 drives the baffle 221 to move, causing the alignment hole 223 to be relatively misaligned with the transverse flow hole 21, thereby changing the actual flow area of ​​the channel. This function gives the equipment excellent process flexibility.

[0169] Stage adaptability: In the initial stage of enzymatic hydrolysis, a large opening is used to accelerate enzyme dispersion and material mixing; in the middle stage, the opening is appropriately narrowed to maintain a suitable circulation flow rate; in the later stage, the opening is further narrowed to reduce shear force and protect product activity.

[0170] Intelligent Adaptability: The real-time turbidity sensor 73 monitors turbidity changes in real time. When the turbidity is high, the opening is enlarged to enhance mass transfer, and when the turbidity decreases, the opening is narrowed to stabilize the reaction, forming an intelligent closed-loop control of "sensing-regulation-feedback".

[0171] Material versatility: For ginkgo protein raw materials with different viscosities and particle sizes, the opening degree can be flexibly selected to match the requirements, and the same equipment can be adapted to a variety of process needs.

[0172] Energy consumption and quality balance: Dynamically switch between large opening (low shear, high flow rate) and small opening (high shear, high turbulence) to achieve the optimal balance between circulation efficiency and product protection.

[0173] Step S3: Intelligent determination of enzymatic hydrolysis endpoint

[0174] The turbidity changes of the enzymatic hydrolysis system are monitored in real time by a real-time turbidity sensor 73. During the enzymatic hydrolysis process, large protein molecules are gradually hydrolyzed into small peptide molecules, and the turbidity of the system changes regularly accordingly. The enzymatic hydrolysis reaction monitoring mechanism 7 includes a temperature sensor 71, a pH sensor 72, and a real-time turbidity sensor 73 integrated and installed on the enzymatic hydrolysis tank 1. When the real-time turbidity sensor 73 detects that the turbidity has reached a preset threshold, it determines that the enzymatic hydrolysis reaction has reached its endpoint and automatically triggers the next filtration process.

[0175] It should be noted that the bottom separator 3 remains closed until the enzymatic hydrolysis process is complete.

[0176] Step S4: In-situ filtration and liquid phase diversion

[0177] After determining that the enzymatic hydrolysis has reached its endpoint, the longitudinal flow hole 32 needs to be opened. It should be noted that the bottom partition plate 3 remains closed until the enzymatic hydrolysis process is complete, and the longitudinal flow hole 32 is normally closed. The longitudinal flow hole 32 corresponds to the closed end of the filter frame 3111. At this time, the top enzymatic hydrolysis zone and the bottom sedimentation zone are not connected.

[0178] When filtration is required, the electromagnetic clutch 315 engages. Power from the second servo motor 61 is transmitted to the torque drive shaft 314 via the bottom rotating shaft 62, causing the torque drive shaft 314 to rotate. The torque head 316 has an angle locking button 317 on its top that rotates and engages with the top of the central base plate 33. During the rotation of the torque drive shaft 314, the torque head 316 causes the flexible connecting cable 317 to tighten, the return spring 313 to contract, and the movable filter screen 311 to slide within the sliding groove 312. The movable filter screen 311 consists of a filter frame 3111 and a detachable filter screen 3112 inserted into one side of the filter frame 3111. One end of the filter frame 3111 is sealed and not installed. When the movable filter screen 311 slides until the detachable filter screen 3112 corresponds to the longitudinal flow hole 32, the filtration channel opens. At this time, the electromagnetic clutch 315 is disengaged, and the angle locking button 317 is rotated and locked to the top of the central base plate 33, maintaining the current position of the movable filter screen 311, so that the removable filter screen 3112 maintains the corresponding relationship with the longitudinal flow hole 32.

[0179] At this point, the enzymatic hydrolysate in the top enzymatic hydrolysis zone is filtered by the adjustable filter plate 31 under gravity. The adjustable filter plate 31 is embedded on both sides of the bottom partition plate 3. During filtration, soluble small molecule peptides (enzymatic hydrolysate phase) pass through the detachable filter screen 3112 of the movable filter screen 311 and enter the bottom sedimentation zone through the longitudinal flow holes 32; while the un-enzymatically hydrolyzed solid residue is retained in the top enzymatic hydrolysis zone. The main function of the detachable filter screen 3112 is to filter large impurities; fine filtration is not required, and membrane filtration is generally performed after centrifugation. Multiple detachable filter screens 3112 can be installed sequentially to switch between different filtration fineness levels.

[0180] After filtration is complete, if it is necessary to close the longitudinal flow hole 32, the electromagnetic clutch 315 will re-engage, the bottom rotating shaft 62 will rotate in the opposite direction, the reset spring 313 will extend and reset, pushing the movable filter screen 311 to slide in the opposite direction, so that the sealing end of the filter screen frame 3111 returns to the position corresponding to the longitudinal flow hole 32, the longitudinal flow hole 32 will close again, blocking the connection between the top enzymatic hydrolysis zone and the bottom sedimentation zone.

[0181] A replacement port 35, corresponding to the movable filter screen 311, is provided through the side wall of the tank body 112. A sealing plate 351 is detachably fixed to the replacement port 35 by bolts 352 for periodic replacement and maintenance of the removable filter screen 3112. Solid residues trapped in the top enzymatic hydrolysis zone are later discharged through the first slag discharge port 15, which is located on the side wall of the tank body 112 and extends to the bottom of the top enzymatic hydrolysis zone.

[0182] Step S5: Precipitation Separation and Collection

[0183] The second stirring mechanism 6 is activated to agitate the enzymatic hydrolysate entering the bottom sedimentation zone. In the second stirring mechanism 6, the second servo motor 61 drives the bottom rotating shaft 62 to rotate. The bottom rotating shaft 62 has second stirring rods 631 evenly distributed circumferentially, which agitate the enzymatic hydrolysate in the bottom sedimentation zone at low speed. The free end of the second stirring rod 631 is equipped with an expanded-diameter stirring head 6311. The outer diameter of the expanded-diameter stirring head 6311 is larger than the diameter of the second stirring rod 631, which increases the disturbance range at the end, effectively eliminating the sedimentation dead zone at the bottom edge of the tank, preventing large-area caking of the sediment at the bottom of the tank, and pushing the sediment towards the second slag discharge port 16.

[0184] With the assistance of the second stirring mechanism 6, fine particles settle in the bottom sedimentation zone, and the sediment is discharged through the second slag discharge port 16, which is located at the lower edge of the tank bottom cover 113. The supernatant in the bottom sedimentation zone is collected to obtain the ginkgo peptide hydrolysate, which is output through the discharge port 14 located on the side wall of the tank body 112.

[0185] Throughout the entire workflow, the support legs 12 are evenly distributed circumferentially on the bottom of the side wall of the tank body 112. The shock-absorbing supports 121 fixedly installed at the ends of each support leg 12 provide stable support and shock absorption for the equipment. The feed inlet 13 is located on the top of the tank cover 111 for feeding raw materials. The tank body 11 is composed of the tank cover 111, the tank body 112, and the tank bottom cover 113 arranged sequentially. The bottom partition plate 3 is composed of a central bottom plate 33 and side wing inclined plates 34 symmetrically arranged on both sides of the central bottom plate 33. The end of the central positioning shaft 51 is movably installed on the upper end of the central bottom plate 33. Each side wing inclined plate 34 is inclined downward towards the corresponding side wall of the tank body 112. The longitudinal flow holes 32 are evenly distributed on the side wing inclined plates 34. The ends of each side driven shaft 53 are movably installed on the corresponding side wing inclined plate 34, and the longitudinal flow holes 32 are located on both sides of each side driven shaft 53 to ensure smooth and unobstructed flow.

[0186] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0187] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A ginkgo peptide ultrasonic enzymatic hydrolysis device, characterized in that, include: The enzymatic hydrolysis tank (1) has a top partition plate (2) and a bottom partition plate (3) arranged vertically inside. The top partition plate (2) divides the upper space of the enzymatic hydrolysis tank (1) into a central enzymatic hydrolysis zone and side enzymatic hydrolysis zones on both sides. The bottom partition plate (3) divides the lower space of the enzymatic hydrolysis tank (1) into a top enzymatic hydrolysis zone and a bottom sedimentation zone. The top partition plate (2) is provided with transverse flow holes (21) at its upper and lower ends to connect the central enzymatic hydrolysis zone and the side enzymatic hydrolysis zone to form an enzymatic hydrolysis circulation path. An adjustable filter plate (31) is embedded in both sides of the bottom partition plate (3), and a longitudinal flow hole (32) is provided on the bottom partition plate (3) on the upper and lower sides corresponding to the adjustable filter plate (31) to guide the enzymatically hydrolyzed liquid phase to the bottom sedimentation area. The first stirring mechanism (5) is set in the top enzymatic hydrolysis zone and is used to drive the material to circulate and enzymatically hydrolyze. The second stirring mechanism (6) is located in the bottom sedimentation zone and is used to stir the sediment and assist in slag discharge. An ultrasonic generator (4) is fixedly installed on the side wall of the enzymatic hydrolysis tank (1) to assist the enzymatic hydrolysis reaction; The enzymatic hydrolysis reaction monitoring mechanism (7) includes a temperature sensor (71), a pH sensor (72) and a real-time turbidity sensor (73) integrated on the enzymatic hydrolysis tank (1). The real-time turbidity sensor (73) is used to monitor the enzymatic hydrolysis process and control the opening timing of the adjustable filter plate (31).

2. The ginkgo peptide ultrasonic enzymatic hydrolysis device according to claim 1, characterized in that, The enzymatic hydrolysis vessel (1) includes: The tank body (11) is composed of a top cover (111), a body (112) and a bottom cover (113) arranged in sequence. The side wall of the body (112) is inlaid with a spirally wound heat exchange tube. Support legs (12) are evenly distributed around the bottom of the side wall of the tank body (112), and shock-absorbing supports (121) are fixedly installed at the ends of each support leg (12). The feed inlet (13) is located on the top of the tank cover (111); The discharge port (14) is located on the side wall of the tank body (112); The first slag discharge port (15) is provided on the side wall of the tank body (112), and the inner end of the first slag discharge port (15) extends to the bottom of the top enzymatic hydrolysis zone; The second slag discharge port (16) is located at the lower edge of the bottom cover (113).

3. The ginkgo peptide ultrasonic enzymatic hydrolysis device according to claim 2, characterized in that, The first stirring mechanism (5) includes: The central positioning shaft (51) is movably set in the center of the tank body (11), its top is movably installed through and on the tank top cover (111), and its bottom is movably installed on the bottom partition plate (3); The first spiral propulsion plate (52) is fixedly sleeved on the central positioning shaft (51); Side driven shafts (53) are arranged in a mirror symmetrical manner on both sides of the central positioning shaft (51). The top of each side driven shaft (53) passes through and is movably installed on the tank top cover (111), and its bottom is movably installed on the bottom partition plate (3). The second spiral propulsion plate (54) is fixedly sleeved on each of the side driven shafts (53); The first stirring group (55) is respectively set at the upper and lower ends of each of the second spiral propulsion plates (54). Each first stirring group (55) consists of a first stirring rod (551) evenly distributed circumferentially on the corresponding side driven shaft (53). A power drive assembly (56) is located at the upper end of the enzymatic hydrolysis tank (1) and is used to drive the central positioning shaft (51) and the side driven shaft (53) to rotate. The first stirring rod (551) has a non-circular cross-section, and each of the first stirring rods (551) is arranged at an angle relative to the horizontal plane. In the same first stirring group (55), the adjacent first stirring rods (551) are of different lengths and are arranged in an alternating pattern of long and short.

4. The ginkgo peptide ultrasonic enzymatic hydrolysis device according to claim 3, characterized in that, The power drive assembly (56) includes: A fixing bracket (561) is fixedly installed on the upper end of the enzymatic hydrolysis vessel (1); The first servo motor (562) is fixedly installed at the lower end of the fixed frame (561), and the output end of the first pulley (563) is fixedly installed. The bottom of the first pulley (563) is fixedly connected to the top of the central positioning shaft (51). The second pulley (564) is fixedly installed on the top of each of the side driven shafts (53); A drive belt (565) is connected between the first pulley (563) and each of the second pulleys (564); Angle-increasing pulleys (566) are respectively disposed between the first pulley (563) and each of the second pulleys (564), and the angle-increasing pulleys (566) overlap the outer side wall of the transmission belt (565) to increase the wrap angle between the transmission belt (565) and the corresponding pulley.

5. The ginkgo peptide ultrasonic enzymatic hydrolysis device according to claim 4, characterized in that, The second stirring mechanism (6) includes: The second servo motor (61) is fixedly installed at the lower end of the enzymatic hydrolysis tank (1); The bottom rotating shaft (62) is movably installed in the bottom sedimentation area, and the bottom end of the bottom rotating shaft (62) is fixedly connected to the output end of the second servo motor (61); The second stirring group (63) consists of second stirring rods (631) that are circumferentially distributed on the bottom rotating shaft (62); The free end of the second stirring rod (631) is provided with an expanded diameter stirring head (632), the outer diameter of which is larger than the diameter of the second stirring rod (631).

6. The ginkgo peptide ultrasonic enzymatic hydrolysis device according to claim 5, characterized in that: The bottom partition plate (3) consists of a central bottom plate (33) and side wing inclined plates (34) symmetrically arranged on both sides of the central bottom plate (33); The end of the central positioning shaft (51) is movably mounted on the upper end of the central base plate (33); Each of the side wing inclined plates (34) is inclined downward toward the corresponding side wall of the tank body (112), and the longitudinal flow holes (32) are evenly distributed on the side wing inclined plates (34); The ends of each of the side driven shafts (53) are movably mounted on the corresponding side wing inclined plate (34), and the longitudinal flow holes (32) are located on both sides of each of the side driven shafts (53).

7. The ginkgo peptide ultrasonic enzymatic hydrolysis device according to claim 6, characterized in that, The adjustable filter plate (31) includes: The movable filter (311) is slidably installed in the sliding groove (312) opened inside the side wing inclined plate (34); A return spring (313) has one end fixedly connected to the side wall of the sliding groove (312) and the other end fixedly connected to the side wall of the movable filter (311). A torque drive shaft (314) is movably installed at the bottom center of the central base plate (33). The bottom end of the torque drive shaft (314) is fixedly connected to the bottom rotating shaft (62) through an electromagnetic clutch (315). A torque head (316) is fixedly installed at the top of the torque drive shaft (314). The top of the torque head (316) is provided with an angle locking button (317) that rotates and engages with the top of the central base plate (33). A flexible connecting cable (317) is fixedly installed at one end of the movable filter screen (311) and at the other end of the cable is fixedly installed on the torque head (316). The movable filter (311) consists of a filter frame (3111) and a detachable filter (3112) inserted into one side of the filter frame (3111); The tank body (112) has a replacement port (35) through which a corresponding movable filter screen (311) is provided. A sealing plate (351) is detachably fixed to the replacement port (35) by bolts (352).

8. The ginkgo peptide ultrasonic enzymatic hydrolysis device according to claim 7, characterized in that: The transverse flow hole (21) is configured as a long groove, and the interior of the top partition plate (2) is provided with an adjustment mechanism (22) corresponding to each transverse flow hole (21). The adjustment mechanism (22) includes: The baffle plate (221) is movably installed in the lifting groove (222) inside the side wall of the top partition plate (2). The baffle plate (221) is provided with alignment holes (223) that correspond one-to-one with each of the transverse flow holes (21). Compression springs (224) are evenly distributed on the upper end of the baffle plate (221), and the compression springs (224) are in a compressed state, and their elastic force always pushes the baffle plate (221) to move upward. A triangular sliding bracket (225) is fixedly installed on the baffle plate (221); An electric telescopic rod (226) is fixedly installed in the middle of the top partition plate (2). An extrusion plate (227) is fixedly installed at the telescopic end of the electric telescopic rod (226). The ends of the extrusion plates (227) slide against the inclined surfaces of the corresponding triangular sliding frames (225). When the electric telescopic rod (226) extends, the squeezing plate (227) pushes the triangular sliding frame (225) and the baffle plate (221) downward, causing the alignment hole (223) and the transverse flow hole (21) to be relatively misaligned, thereby changing the actual flow area of ​​the transverse flow hole (21).

9. The ginkgo peptide ultrasonic enzymatic hydrolysis device according to claim 8, characterized in that: An outer edge guide block (2231) is inlaid on the upper edge of the alignment hole (223), and a lower protrusion (2232) is provided at the lower center of the outer edge guide block (2231). A bottom blocking block (2233) is inlaid on the bottom edge of the transverse flow hole (21), and an upper protrusion (2234) is provided at the upper center of the bottom blocking block (2233). The outer guide block (2231) and the bottom shield block (2233) form a flow channel (2235) through which fluid can pass, and the flow channel (2235) has a trend of first contraction and then expansion along the fluid direction.

10. The enzymatic hydrolysis method of a ginkgo peptide ultrasonic enzymatic hydrolysis device according to claim 9, characterized in that, Includes the following steps: S1: Add ginkgo protein raw material and water into the top enzymatic hydrolysis zone of the enzymatic hydrolysis tank (1) in proportion, start the first stirring mechanism (5) to stir and mix, and monitor and adjust the temperature and pH value of the enzymatic hydrolysis system to the preset range through the temperature sensor (71) and the pH sensor (72). S2: Add protease to the top enzymatic hydrolysis zone, start the ultrasonic generator (4) to apply ultrasonic waves, and under the drive of the first stirring mechanism (5), the material in the top enzymatic hydrolysis zone circulates between the central enzymatic hydrolysis zone and the side enzymatic hydrolysis zone through the transverse flow hole (21) at the upper end of the top partition plate (2) to carry out ultrasonic-assisted enzymatic hydrolysis reaction. S3: The turbidity change of the enzymatic hydrolysis system is monitored in real time by the real-time turbidity sensor (73). When the turbidity reaches the preset threshold, the enzymatic hydrolysis reaction is determined to have reached the endpoint. S4: Open the adjustable filter plates (31) on both sides of the bottom partition plate (3) so that the enzymatic hydrolysate in the top enzymatic hydrolysis zone is filtered by the adjustable filter plate (31) and enters the bottom sedimentation zone through the longitudinal flow hole (32), and the solid residue is trapped in the top enzymatic hydrolysis zone. S5: Start the second stirring mechanism (6) to stir the enzymatic hydrolysate entering the bottom sedimentation zone, so that the precipitate settles in the bottom sedimentation zone and is discharged through the second slag discharge port (16). Collect the supernatant in the bottom sedimentation zone to obtain the ginkgo peptide enzymatic hydrolysate.

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