Liquid path structure of blood cell analyzer and blood cell analyzer

Abstract

This application provides a waste liquid treatment method for a hematology analyzer. The hematology analyzer includes a premixing tank, a reaction tank, a sheath flow impedance detection component, an optical detection component, and a pressure source. The waste liquid treatment method includes: preparing a first sample solution through the premixing tank and / or preparing a second sample solution through the reaction tank; receiving first waste liquid generated during sample detection of the first sample solution by the sheath flow impedance detection component through the premixing tank, and / or receiving second waste liquid generated during sample detection of the second sample solution by the optical detection component through the reaction tank; and discharging the first waste liquid in the premixing tank and / or the second waste liquid in the reaction tank from the hematology analyzer through the pressure source. The waste liquid from the sheath flow impedance detection component and / or the optical detection component is discharged into the premixing tank and / or the reaction tank respectively, eliminating the need for an additional independent waste liquid tank for waste liquid treatment. This results in a smaller hematology analyzer, higher waste liquid treatment efficiency, and higher detection efficiency.

Description

[0001] This application is a divisional application of Chinese application filed on August 9, 2023, with application number 2023110020759, entitled "A waste liquid treatment method and a blood cell analyzer using the method". Technical Field

[0002] The present invention relates to the field of in vitro diagnostic technology, and in particular to a waste liquid treatment method and a blood cell analyzer using the method. Background Technology

[0003] Existing five-part differential hematology analyzers are used to analyze formed elements in human blood, such as counting red blood cells and classifying white blood cells. Sheath flow technology enables white blood cell classification. Specifically, a capillary tube is aligned with the optical flow chamber, and a cell suspension is ejected from the capillary. Simultaneously, it flows through the sensitive area along with sheath fluid flowing from the surrounding area, ensuring that the cell suspension forms a single-celled stream in the center, surrounded by sheath fluid. Sheath flow impedance technology enables red blood cell counting. Specifically, a capillary tube is aligned with a sapphire aperture, and a cell suspension is ejected from the capillary. Simultaneously, it flows through the sapphire aperture along with sheath fluid flowing from the surrounding area, ensuring that the cell suspension forms a single-celled stream in the center, surrounded by sheath fluid.

[0004] Currently, red blood cell counting and white blood cell differential analysis generally require incubation with diluent in a reaction tank before being fed into the corresponding detection components. Both red blood cell counting and white blood cell differential analysis typically require a stable sheath flow within the detection components to ensure reliable results. Existing technologies usually employ two waste liquid tanks to receive waste liquid generated during the detection process and maintain a stable sheath flow. Waste liquid generated in the reaction tank is typically pre-collected into one waste liquid tank using negative pressure, and a second waste liquid tank, connected to ambient atmospheric pressure, is used to collect waste liquid discharged through a positive pressure source during the detection process, preventing interference with the sheath flow. However, using two waste liquid tanks to handle waste liquid generated during the detection process requires a large number of tanks, resulting in a larger overall size of the hematology analyzer. Furthermore, the large number of waste liquid tanks necessitates matching fluid delivery tubing, leading to an increase in the amount of cleaning solution required for the tubing and consequently, lower overall detection efficiency of the hematology analyzer. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide a waste liquid treatment method and a blood cell analyzer using the method, so as to solve the problems of large overall size and low overall detection efficiency of existing blood cell analyzers due to the large number of waste liquid pools required.

[0006] One aspect of the present invention provides a blood cell analyzer, comprising:

[0007] It is applied to a blood cell analyzer, which includes a premixing cell, a reaction cell, a sheath flow impedance detection component, an optical detection component, and a pressure source;

[0008] The method includes:

[0009] The first sample solution is prepared by the premixing cell and / or the second sample solution is prepared by the reaction cell;

[0010] The premixing cell receives the first waste liquid generated during the process of the sheath flow impedance detection component detecting the first sample liquid, and / or the reaction cell receives the second waste liquid generated during the process of the optical detection component detecting the second sample liquid.

[0011] The blood cell analyzer is discharged from the premixing tank and / or the reaction tank by the pressure source.

[0012] Optionally, the blood cell analyzer further includes: a sample preparation component, a sample solution dispensing component, a sixth liquid tube, an eighth liquid tube, and a ninth liquid tube.

[0013] The sample preparation component is connected to the premixing cell and the reaction cell; the sample liquid pushing component is connected to the sheath flow impedance detection component and the optical detection component; the sample preparation component is connected to one end of the eighth liquid tube; the other end of the eighth liquid tube is connected to one end of the ninth liquid tube; the other end of the ninth liquid tube is connected to one end of the sixth liquid tube; and the other end of the sixth liquid tube is connected to the premixing cell.

[0014] The method further includes:

[0015] Before testing the first sample solution and / or the second sample solution, the sample preparation component aspirates the first sample solution from the premixing tank into a liquid tube between the premixing tank and the sample preparation component, and / or, the sample preparation component aspirates the second sample solution from the reaction tank into a liquid tube between the reaction tank and the sample preparation component.

[0016] The method further includes:

[0017] During the process of the sample preparation component drawing the first sample liquid and / or the second sample liquid for testing, the waste liquid generated when the sample preparation component draws the first sample liquid and / or the second sample liquid enters the premixing tank sequentially through the eighth liquid tube, the ninth liquid tube and the sixth liquid tube.

[0018] Optionally, the blood cell analyzer further includes a first switching element and a first liquid tube, the sample preparation component is connected to the reaction cell through the first switching element; the liquid inlet of the optical detection component is connected to the first switching element, the liquid outlet of the optical detection component is connected to one end of the first liquid tube, and the other end of the first liquid tube is connected to the reaction cell;

[0019] The second waste liquid generated during the process of receiving the second sample liquid by the optical detection component through the reaction cell further includes:

[0020] When the first on / off switch is turned on, the optical detection component detects the second sample liquid;

[0021] The sample liquid pushing component pushes the second waste liquid generated during the detection of the second sample liquid by the optical detection component into the first liquid tube;

[0022] When the first on / off switch is closed, the sample liquid pushing assembly injects the second waste liquid in the first liquid tube into the reaction tank.

[0023] Optionally, the length of the first liquid tube is less than or equal to 60 mm.

[0024] Optionally, the blood cell analyzer further includes a diluent pool, a positive pressure source, a second switch, a third switch, and a fourth switch.

[0025] The diluent pool is connected to the positive pressure source and the second switching element; the second switching element is connected to the third switching element; the third switching element is connected to the sheath current impedance detection component; the second switching element is connected to the fourth switching element; and the fourth switching element is connected to the optical detection component.

[0026] The method further includes:

[0027] When the second and third switching devices are turned on, the diluent in the diluent pool is injected into the sheath flow impedance detection component via the second and third switching devices, and a stable sheath flow is formed in the sheath flow impedance detection component.

[0028] When the second and fourth switches are turned on, the diluent in the diluent pool is injected into the optical detection assembly via the second and fourth switches, and a stable sheath flow is formed in the optical detection assembly.

[0029] Optionally, the sheath flow impedance detection assembly includes a sheath flow impedance detection cell, and the sheath flow impedance detection cell includes a pre-cell; the blood cell analyzer further includes: a fifth switching element; the fifth switching element connects the pre-cell and one end of the eighth liquid tube;

[0030] The first waste liquid generated during the process of receiving the first sample liquid from the sheath current impedance detection cell through the premixed cell for sample detection also includes:

[0031] When the second and fifth switching devices are turned on, and the positive pressure source is turned on, the first waste liquid generated during the sample detection process of the first sample liquid by the sheath flow impedance detection cell enters the premixing cell from the front pool sequentially through the fifth switching device, the eighth liquid pipe, the ninth liquid pipe and the sixth liquid pipe under the action of the positive pressure and the sample liquid pushing component.

[0032] Optionally, the sheath flow impedance detection cell further includes a rear chamber, the front chamber and the rear chamber are connected through a sapphire hole, and the blood cell analyzer further includes: a sixth switching element; the sixth switching element connects the rear chamber and the second switching element, and the rear chamber is connected to one end of the ninth liquid tube;

[0033] The first waste liquid generated during the process of receiving the first sample liquid from the sheath current impedance detection cell through the premixed cell for sample detection also includes:

[0034] When the second and sixth switching devices are turned on, the positive pressure source is turned on. During the process of the sheath flow impedance detection cell performing sample detection on the first sample liquid, the first waste liquid generated is introduced into the premixing cell from the rear cell through the ninth liquid pipe and the sixth liquid pipe under positive pressure and the sample liquid pushing component.

[0035] Optionally, the pressure source includes a liquid pump, which is connected to the premixing tank, the reaction tank, and an external waste liquid collection assembly located outside the blood cell analyzer.

[0036] The blood cell analyzer further includes the method of discharging the first waste liquid in the premixing tank and / or the second waste liquid in the reaction tank through the pressure source:

[0037] Turn on the liquid pump to directly discharge the first waste liquid in the premixing tank and / or the second waste liquid in the reaction tank from the blood cell analyzer, so that the first waste liquid and / or the second waste liquid are injected into the external waste liquid collection assembly.

[0038] Optionally, the blood cell analyzer further includes: at least one metering pump assembly, a metering pump switching gas valve, and a metering pump switching liquid valve.

[0039] One end of the at least one metering pump assembly is connected to the diluent tank via the positive pressure source and the metering pump switching valve, and the other end of the at least one metering pump assembly is connected to the premixing tank via the metering pump switching valve;

[0040] The method further includes:

[0041] When the air chamber of the at least one metering pump assembly is connected to atmospheric pressure and the positive pressure source is activated, the metering pump switching air valve and the metering pump switching liquid valve are in a de-energized state, and the metering pump switching air valve switches to the diluent tank to connect the at least one metering pump assembly; the positive pressure source drives the diluent in the diluent tank to enter the liquid chamber of the at least one metering pump assembly;

[0042] The air chamber of the at least one metering pump assembly is connected to the positive pressure source. When the positive pressure source is activated, the metering pump switching air valve and the metering pump switching liquid valve are energized. The metering pump switching air valve switches to the at least one metering pump assembly connected to the premixing cell. The positive pressure source drives the liquid chamber of the at least one metering pump assembly to draw up the diluent and enter the premixing cell through the metering pump switching liquid valve to mix with the sample to be tested to obtain the first sample solution.

[0043] One aspect of the present invention provides a blood cell analyzer, the blood cell analyzer comprising: a premixing tank, a reaction tank, a sheath flow impedance detection component, an optical detection component, and a pressure source; the premixing tank is connected to the sheath flow impedance detection component, the reaction tank is connected to the optical detection component; the pressure source is connected to the sheath flow impedance detection component and the optical detection component; the blood cell analyzer performs the waste liquid treatment method as described in any of the above embodiments at least through the premixing tank and the sheath flow impedance detection component, and / or, the reaction tank and the optical detection component.

[0044] This invention provides a waste liquid treatment method and a hematology analyzer using this method. The hematology analyzer includes a premixing tank, a reaction tank, a sheath flow impedance detection component, an optical detection component, and a pressure source. The method includes: preparing a first sample solution through the premixing tank and / or preparing a second sample solution through the reaction tank; receiving a first waste liquid generated during sample detection of the first sample solution by the sheath flow impedance detection component through the premixing tank, and / or receiving a second waste liquid generated during sample detection of the second sample solution by the optical detection component through the reaction tank; discharging the first waste liquid in the premixing tank and / or the second waste liquid in the reaction tank from the hematology analyzer through the pressure source; and discharging the waste liquid from the sheath flow impedance detection component into the premixing tank and / or the waste liquid from the optical detection component into the reaction tank. This eliminates the need for an additional independent waste liquid tank for waste liquid treatment, resulting in a smaller overall size of the hematology analyzer, higher waste liquid treatment efficiency, and higher overall detection efficiency.

[0045] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments, but this is not intended to limit the present invention. Attached Figure Description

[0046] Figure 1 This schematically illustrates an example flowchart of the overall process for implementing the waste liquid treatment method of the present invention;

[0047] Figure 2 This schematically illustrates an example flowchart of the sample preparation component aspirating sample liquid in the waste liquid treatment method of the present invention;

[0048] Figure 3 This schematically illustrates an example flow chart of the second waste liquid injection reaction tank in the waste liquid treatment method of the present invention;

[0049] Figure 4 A schematic diagram of the fluid circuit structure of a blood cell analyzer according to an embodiment of the present invention is shown.

[0050] Figure Descriptions: 101. Sample preparer; 102. Liquid pump; 103. Premixing cell; 104. Reaction cell; 105. Sheath flow impedance detection assembly; 106. Second detection device; 107. Positive pressure source; 108. Metering pump switching gas valve; 109. Metering pump switching liquid valve; 110. Sample preparation assembly; 111. Second on / off element; 112. Fourth on / off element; 113. Third on / off element; 114. Sixth on / off element; 115. Fifth on / off element; 116. 117. Quantitative pump assembly; 118. Reagent quantitative assembly; 119. Sample liquid propulsion assembly; 120. External waste liquid collection assembly; 121. Diluent pool; 122. Sheath flow impedance sample preparation valve; 123. First on / off element; 201. First liquid tube; 202. Second liquid tube; 203. Third liquid tube; 204. Fourth liquid tube; 205. Fifth liquid tube; 206. Sixth liquid tube; 207. Seventh liquid tube; 208. Eighth liquid tube; 209. Ninth liquid tube. Detailed Implementation

[0051] The present application will now be described in further detail with reference to the accompanying drawings and embodiments. It should be particularly noted that the following embodiments are for illustrative purposes only and do not limit the scope of the application. Similarly, the following embodiments are only some, not all, embodiments of the present application, and all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of the present application.

[0052] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a mutually exclusive, independent, or alternative embodiment. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0053] In the description of this application, it is necessary to specify that, unless otherwise expressly stated and limited, the terms "installation," "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or a connection through an intermediate medium. Those skilled in the art will understand the specific meanings of the above terms within the context of this application.

[0054] Please see Figure 1 and Figure 4 , Figure 1 This schematically illustrates an example flowchart of the overall process for implementing the waste liquid treatment method of the present invention; Figure 4 A schematic diagram of the liquid circuit structure of a hematology analyzer according to an embodiment of the present invention is shown. This application provides a waste liquid treatment method applied to a hematology analyzer, which includes a premixing tank 103, a sheath flow impedance detection component 105, a reaction tank 104, an optical detection component 106, and a pressure source. It is understood that the flowchart in this method embodiment is not intended to limit the order of execution steps. The following description uses hematology analysis as the execution subject as an example:

[0055] Step S100: Prepare a first sample solution through the premixing cell and / or prepare a second sample solution through the reaction cell.

[0056] In this application, the hematology analyzer also includes a sample preparer 101, which is used to aspirate the sample to be tested from a test tube. The sample preparer 101 can be a sampling needle. In this application, the sample to be tested is aspirated from the test tube using the sample preparer 101, and then the sample aspirated by the sample preparer 101 is injected into the premixing chamber 103 and the reaction chamber 104, respectively. The hematology analyzer also includes a diluent chamber 120 and a reagent quantification component 117. The diluent chamber 120 is connected to the premixing chamber 103 and provides diluent. The first sample solution is prepared by the following operation: the diluent chamber 120 injects diluent into the premixing chamber 103, and the diluent is mixed with the sample to be tested in the premixing chamber 103 to prepare the first sample solution. The reagent quantification component 117 is connected to the reaction chamber 104. The second sample solution is prepared by the following operation: a quantitative reagent is added to the reaction chamber 104 by the reagent quantification component 117, and the quantitative reagent is mixed and reacted with the sample to be tested in the reaction chamber 104 to prepare the second sample solution. The sheath flow impedance detection component 105 is used at least for red blood cell counting; the optical detection component 106 is used at least for white blood cell differential detection.

[0057] The hematology analyzer also includes: a positive pressure source 107, a metering pump switching air valve 108, at least one metering pump assembly 116, and a metering pump switching liquid valve 109. The metering pump assembly 116 consists of a diaphragm, an air chamber, and a liquid chamber. A diluent pool 120 is connected to one end of the positive pressure source 107, and the other end of the positive pressure source 107 is connected to one end of the metering pump switching air valve 108. The other end of the metering pump switching air valve 108 is connected to the air chamber of the metering pump assembly 116 for switching the air pressure of the metering pump assembly 116. The liquid chamber of the metering pump assembly 116 is connected to one end of the metering pump switching liquid valve 109, which is used for switching the flow path of the liquid chamber of the metering pump assembly 116. Both the metering pump switching air valve 108 and the metering pump switching liquid valve 109 are three-way solenoid valves.

[0058] The waste liquid treatment method further includes:

[0059] When the air chamber of the at least one metering pump assembly 116 is connected to atmospheric pressure, the positive pressure source 107 is activated, the metering pump switching air valve 108 and the metering pump switching liquid valve 109 are in a de-energized state, and the metering pump switching air valve 108 switches to the diluent pool 120 to connect the at least one metering pump assembly 116; the positive pressure source 107 drives the diluent in the diluent pool 120 into the liquid chamber of the at least one metering pump assembly 116;

[0060] The air chamber of the at least one metering pump assembly 116 is connected to the positive pressure source 107. When the positive pressure source 107 is activated, the metering pump switching air valve 108 and the metering pump switching liquid valve 109 are energized. The metering pump switching air valve 108 switches to the at least one metering pump assembly 116 connected to the premixing tank 103. The positive pressure source 107 drives the liquid chamber of the at least one metering pump assembly 116 to draw diluent into the premixing tank 103 through the metering pump switching liquid valve 109, so as to mix with the sample to be tested to obtain the first sample solution.

[0061] This can be understood as follows: When the metering pump switching air valve 108 and metering pump switching liquid valve 109 are in a de-energized state, the air chamber of the metering pump assembly 116 is connected to atmospheric pressure, and the positive pressure source 107 drives the diluent in the diluent pool 120 to enter the liquid chamber of the metering pump assembly 116; when the metering pump switching air valve 108 and metering pump switching liquid valve 109 are in a energized state, the air chamber of the metering pump assembly 116 is connected to the positive pressure source 107, and the positive pressure source 107 drives the diluent drawn by the liquid chamber of the metering pump assembly 116 to enter the premixing pool 103 through the metering pump switching liquid valve 109, so as to mix with the sample to be tested to obtain the first sample solution.

[0062] In an exemplary embodiment, one end of the at least one metering pump assembly 116 is connected to the diluent reservoir 120, and the other end of the at least one metering pump assembly 116 is connected to the premixing reservoir 103. The number of the at least one metering pump assembly 116 is a preset number, and the volume of the preset number of metering pumps can meet the total amount of diluent required for the premixing reservoir 103 to prepare the first sample solution in a single operation when the preset number of metering pumps are turned on simultaneously. Alternatively, the number of the at least one metering pump assembly 116 is one, and when the metering pump is turned on only once to deliver the diluent to the premixing reservoir 103, the volume of the metering pump meets the total amount of diluent required for the premixing reservoir 103 to prepare the first sample solution in a single operation. For example, preparing the first sample solution for a single test requires 1.5 ml of diluent, using a single metering pump with a volume of 0.5 ml, that is, the number of at least one metering pump assembly 116 can be three, that is, three 0.5 ml metering pumps connected in parallel to form a metering assembly with a total volume of 1.5 ml. Three aspiration operations of the metering pump assembly 116 are required during a single test. In another embodiment, a single metering pump with a volume of at least 1.5 ml can be used. Both of these methods reduce the time impact of multiple aspirations by the metering pump assembly 116, thereby improving detection efficiency. In other embodiments, the total volume of the metering pump assembly 116 is greater than 1.5 mL.

[0063] In this application, the hematology analyzer further includes a sheath flow impedance sample preparation valve 121 and a seventh liquid tube 207. The premixing cell 103 includes a first port, a second port, and a third port, each independently configured. The second port of the premixing cell 103 is connected to the other end of a sixth liquid tube 206. One end of the sixth liquid tube 206 is connected to one end of the sheath flow impedance sample preparation valve 121, and the other end of the sheath flow impedance sample preparation valve 121 is connected to one end of the seventh liquid tube 207. The other end of the seventh liquid tube 207 is connected to a sample preparation assembly 110. When the sheath flow impedance sample preparation valve 121 is opened, the sample preparation assembly 110 provides negative pressure, allowing the first sample solution prepared in the premixing cell 103 to enter the seventh liquid tube 207 via the sheath flow impedance sample preparation valve 121, thus completing the preparation of the first sample solution before sample detection. The hematology analyzer further includes a first switching element 122, a second liquid tube 202, a third liquid tube 203, and a fourth liquid tube 204. The reaction chamber 104 includes a first end, a second end, and a third end. The second end of the reaction chamber 104 is connected to one end of the second liquid tube 202. One end of the second liquid tube 202 is connected to one end of the first switching element 122. The other end of the first switching element 122 is connected to one end of the third liquid tube 203. The other end of the third liquid tube 203 is connected to one end of the fourth liquid tube 204. The other end of the fourth liquid tube 204 is connected to the sample preparation assembly 110. When the first switching element 122 is turned on, the sample preparation assembly 110 provides negative pressure, allowing the second sample solution prepared in the reaction chamber 104 to sequentially pass through the second liquid tube 202 and the first switching element 122 into the third liquid tube 203, thus completing the preparation of the second sample solution before sample detection.

[0064] In embodiments of this application, the blood cell analyzer further includes a fifth liquid tube 205, one end of which is connected to the sample preparation assembly 110, and the other end of which is connected to one end of the eighth liquid tube 208. After preparing the first sample solution and / or the second sample solution, the sample preparation assembly 110 sequentially introduces the aspirated waste liquid into the premixing tank 103 via the fifth liquid tube 205, the eighth liquid tube 208, the ninth liquid tube 209, and the sixth liquid tube 206.

[0065] Combination Figure 2 and Figure 4The method further includes treating the waste liquid generated during the sample preparation stage, specifically including steps S200 to S202, wherein: in step S200, before detecting the first sample liquid and / or the second sample liquid, the sample preparation component draws the first sample liquid from the premixing tank into a liquid tube between the premixing tank and the sample preparation component, and / or, the sample preparation component draws the second sample liquid from the reaction tank into a liquid tube between the reaction tank and the sample preparation component; in step S202, during the process of the sample preparation component drawing the first sample liquid and / or the second sample liquid for detection, the waste liquid generated when the sample preparation component draws the first sample liquid and / or the second sample liquid enters the premixing tank sequentially through the eighth liquid tube, the ninth liquid tube, and the sixth liquid tube. In this embodiment, during the sample preparation stage, the waste liquid generated by the sample preparation component 110 is injected into the premixing tank 103. There is no need to add an additional waste liquid tank inside the machine. Furthermore, during the process of injecting the waste liquid into the premixing tank 103, the fifth liquid pipe 205, the eighth liquid pipe 208, the ninth liquid pipe 209, and the sixth liquid pipe 206 through which the waste liquid flows will not be contaminated by the waste liquid, thus avoiding inaccurate test results for the sample.

[0066] To further reduce the size of the hematology analyzer, in an exemplary embodiment, the hematology analyzer further includes: a sample preparation component 110, a sample solution propulsion component 118, a sixth liquid tube 206, an eighth liquid tube 208, and a ninth liquid tube 209. The sample preparation component 110 is connected to the premixing cell 103 and is used to aspirate the first sample solution in the premixing cell 103 into the liquid tube between the premixing cell 103 and the sample preparation component 110. The sample preparation component 110 is connected to the reaction cell 104 and is used to aspirate the second sample solution in the reaction cell 104 into the liquid tube between the reaction cell 104 and the sample preparation component 110. The sample solution propulsion component 118 is connected to the sheath flow impedance detection component 105 and is used to propel the first sample solution in the liquid tube between the premixing cell 103 and the sample preparation component 110 into the sheath flow impedance detection component 105 for sample detection. The sample liquid pushing component 118 is connected to the optical detection component 106. The sample liquid pushing component 118 is used to push the second sample liquid in the liquid tube between the reaction cell 104 and the sample preparation component 110 into the optical detection component 106 for sample detection. The premixing cell 103 is also used to receive the waste liquid generated by the sample preparation component 110 during the sample detection of the first sample liquid and / or the second sample liquid. The sample preparation component 110 is connected to one end of the eighth liquid tube 208, the other end of the eighth liquid tube 208 is connected to one end of the ninth liquid tube 209, the other end of the ninth liquid tube 209 is connected to one end of the sixth liquid tube 206, and the other end of the sixth liquid tube 206 is connected to the premixing cell 103. The waste liquid generated when the sample preparation component 110 aspirates the first sample liquid and / or the second sample liquid enters the premixing cell 103 sequentially through the eighth liquid tube 208, the ninth liquid tube 209, and the sixth liquid tube 206. In this application, the premixing cell 103 and the reaction cell 104 share a sample preparation component 110, and the sheath flow impedance detection component 105 and the optical detection component 106 share a sample liquid propulsion component 118. Sharing devices with the same function reduces the control complexity of the blood cell analyzer and saves equipment costs.

[0067] Step S102: Receive the first waste liquid generated during the sample detection process of the first sample liquid by the sheath flow impedance detection component through the premixing cell, and / or receive the second waste liquid generated during the sample detection process of the second sample liquid by the optical detection component through the reaction cell.

[0068] To further simplify the liquid circuit connection structure and reduce the size of the hematology analyzer, in an exemplary embodiment, the hematology analyzer further includes a first liquid tube 201. The sample preparation component 110 is connected to the reaction cell 104 via the first on / off element 122. The inlet end of the optical detection component 106 is connected to the first on / off element 122, and the outlet end of the optical detection component 106 is connected to one end of the first liquid tube 201. The other end of the first liquid tube 201 is connected to the reaction cell 104. The second waste liquid generated by the optical detection component 106 is injected into the reaction cell 104 through the first liquid tube 201. The optical detection component 106 includes a flow chamber, the inlet end of which is connected to a third liquid tube 203. The inlet end of the flow chamber is connected to the other end of the first on / off element 122 via the third liquid tube 203. The first liquid tube 201 is connected to a second liquid tube 202, and the first liquid tube 201 is connected to the reaction cell 104 via the second liquid tube 202. The second waste liquid in the optical detection component 106 enters the reaction tank 104 sequentially through the liquid outlet of the flow chamber, the first liquid pipe 201, the second liquid pipe 202, and the second end of the reaction tank 104.

[0069] This can be understood as yes, please refer to [link / reference]. Figure 3 and Figure 4 The second waste liquid generated during the sample detection process of the second sample liquid by the optical detection component in step S102 can also be achieved through the following operations: Step S300, the first on / off device is turned on, and the optical detection component detects the second sample liquid; Step S302, the sample liquid pushing component pushes the second waste liquid generated during the detection process of the second sample liquid by the optical detection component into the first liquid tube; Step S304, the first on / off device is turned off, and the sample liquid pushing component injects the second waste liquid in the first liquid tube into the reaction pool. In this embodiment, during the sample detection process of the second sample liquid, the second waste liquid generated by the optical detection component 106 is injected into the reaction pool 104, eliminating the need for an additional waste liquid pool inside the machine. Furthermore, during the injection of waste liquid into the reaction pool 104, the first liquid tube 201 and the second liquid tube 202 through which the waste liquid flows will not be contaminated by the waste liquid, thus avoiding inaccurate detection results.

[0070] In an exemplary embodiment, the length of the first liquid tube 201 is less than or equal to 60 mm. In this application, the reaction tank 104 uses the same section of the second liquid tube 202 during the second sample preparation stage and the second waste liquid collection stage. Considering factors such as detection efficiency, internal liquid tube space layout, equipment cost, and power supply, the length of the first liquid tube 201 is set to be less than or equal to 60 mm. This helps to quickly input the second waste liquid into the reaction tank 104 under the premise of a suitable internal liquid tube space layout and power supply, thereby improving the overall detection efficiency.

[0071] In an exemplary embodiment, the sheath flow impedance detection component 105 includes a sheath flow impedance detection cell, which includes a pre-cell. The blood cell analyzer further includes a fifth switching element 115. The fifth switching element 115 connects the pre-cell and one end of the eighth liquid pipe 208. The other end of the eighth liquid pipe 208 is connected to one end of the ninth liquid pipe 209. The other end of the ninth liquid pipe 209 is connected to one end of the sixth liquid pipe 206. The other end of the sixth liquid pipe 206 is connected to the premixing tank 103. When the second switching element 111 and the fifth switching element 115 are opened, under the action of the positive pressure source 107, the first waste liquid in the sheath flow impedance detection cell enters the premixing tank 103 from the pre-cell sequentially through the fifth switching element 115, the eighth liquid pipe 208, the ninth liquid pipe 209, and the sixth liquid pipe 206.

[0072] It can be understood that the first waste liquid generated during the process of receiving the first sample liquid from the sheath flow impedance detection cell in the premixing cell during sample detection of the first sample liquid in step S102 can also be obtained through the following operation: opening the second switch and the fifth switch, opening the positive pressure source, and the first waste liquid generated during the process of the sheath flow impedance detection cell performing sample detection of the first sample liquid enters the premixing cell from the front cell sequentially through the fifth switch, the eighth liquid pipe, the ninth liquid pipe and the sixth liquid pipe under the action of the positive pressure and the sample liquid pushing component. In this application, after the sheath flow impedance of the first sample liquid is tested, under the positive pressure of the positive pressure source 107, the waste liquid generated in the forepool enters the premixing tank 103 sequentially through the fifth switch 115, the eighth liquid pipe 208, the ninth liquid pipe 209 and the sixth liquid pipe 206. There is no need to add a new waste liquid tank in the machine. Moreover, during the process of the waste liquid being injected into the premixing tank 103, the eighth liquid pipe 208, the ninth liquid pipe 209 and the sixth liquid pipe 206 through which the waste liquid flows will not be contaminated by the waste liquid, which would cause inaccurate test results for the sample.

[0073] In an exemplary embodiment, the sheath flow impedance detection cell further includes a rear chamber, and the front chamber and the rear chamber are connected through a sapphire hole. The blood cell analyzer further includes a sixth switching element 114. The sixth switching element 114 connects the rear chamber and the second switching element 111, and the rear chamber is connected to one end of the ninth liquid pipe 209. When the second switching element 111 and the sixth switching element 114 are open, under the action of the positive pressure source 107, the first waste liquid of the sheath flow impedance detection cell enters the premixing tank 103 from the rear chamber sequentially through the ninth liquid pipe 209 and the sixth liquid pipe 206.

[0074] It can be understood that, in other exemplary embodiments, the first waste liquid generated during the process of receiving the first sample liquid from the sheath flow impedance detection cell through the premixing cell in step S102 can also be obtained by the following operation: opening the second switch and the sixth switch, opening the positive pressure source, and the first waste liquid generated during the process of the sheath flow impedance detection cell testing the first sample liquid enters the premixing cell from the rear cell through the ninth liquid pipe and the sixth liquid pipe in sequence under positive pressure and the sample liquid pushing component. In this application, after sheath flow impedance detection of the first sample solution, under the positive pressure of the positive pressure source 107, the waste liquid generated in the rear chamber enters the premixing tank 103 sequentially through the ninth liquid pipe 209 and the sixth liquid pipe 206. There is no need to add a new waste liquid tank inside the machine. Furthermore, during the process of injecting waste liquid into the premixing tank 103, the ninth liquid pipe 209 and the sixth liquid pipe 206 through which the waste liquid flows will not be contaminated by the waste liquid, thus avoiding inaccurate test results. Moreover, the waste liquid injected into the premixing tank 103 by the front and rear chambers shares the ninth liquid pipe 209 and the sixth liquid pipe 206, eliminating the need to set up independent delivery pipes for the waste liquid discharge of the front and rear chambers. This reduces the complexity of the liquid circuit structure of the blood cell analyzer and helps to reduce the size of the blood cell analyzer.

[0075] To further reduce the size of the device, in an exemplary embodiment, the blood cell analyzer further includes a second switching element 111, a third switching element 113, and a fourth switching element 112. The diluent pool 120 is connected to the positive pressure source 107 and the second switching element 111, the second switching element 111 is connected to the third switching element 113, and the third switching element 113 is connected to the sheath current impedance detection component 105. When the second switching element 111 and the third switching element 113 are open, the diluent pool 120 provides diluent... The released liquid forms a stable sheath flow in the sheath flow impedance detection assembly 105 via the second switch 111 and the third switch 113; the second switch 111 is connected to the fourth switch 112, and the fourth switch 112 is connected to the optical detection assembly 106; when the second switch 111 and the fourth switch 112 are open, the diluent provided by the diluent pool 120 forms a stable sheath flow in the optical detection assembly 106 via the second switch 111 and the fourth switch 112.

[0076] It can be understood that a stable sheath flow can be obtained through the following operations: opening the second and third switches, the diluent in the diluent pool is injected into the sheath flow impedance detection pool through the second and third switches, and a stable sheath flow is formed in the sheath flow impedance detection pool; opening the second and fourth switches, the diluent in the diluent pool is injected into the optical detection component through the second and fourth switches, and a stable sheath flow is formed in the optical detection component.

[0077] The stable sheath current in the sheath current impedance detection component 105 is formed through the following operation:

[0078] When the second switch 111 and the third switch 113 are opened, under the positive pressure provided by the positive pressure source 107, the diluent in the diluent pool 120 enters the sheath flow impedance detection component 105 to form a stable sheath flow. The stable sheath flow envelops the first sample solution and performs red blood cell counting detection within the sheath flow impedance detection component 105.

[0079] The stable sheath current in the sheath current impedance detection component 105 is formed through the following operation:

[0080] When the second switch 111 and the fourth switch 112 are opened, under the positive pressure provided by the positive pressure source 107, the diluent in the diluent pool 120 enters the optical detection component 106 to form a stable sheath flow. The stable sheath flow envelops the second sample solution and performs white blood cell classification detection within the optical detection component 106.

[0081] In this embodiment, the stable sheath flow in both the sheath flow impedance detection component 105 and the optical detection component 106 is achieved by supplying the same dilution pool 120 and controlling the same second on / off element 111, which reduces the complexity of the equipment's liquid circuit structure and further reduces the overall size of the equipment.

[0082] Step S104: The first waste liquid in the premixing tank and / or the second waste liquid in the reaction tank are discharged from the blood cell analyzer by the pressure source.

[0083] In an exemplary embodiment, the pressure source includes a liquid pump 102, which is connected to the premixing tank 103 and the reaction tank 104 respectively. The liquid pump 102 is also connected to an external waste liquid collection assembly 119 located outside the hematology analyzer. In this application, step S104 further includes: turning on the liquid pump to directly discharge the first waste liquid in the premixing tank and / or the second waste liquid in the reaction tank from the hematology analyzer, so that the first waste liquid and / or the second waste liquid are injected into the external waste liquid collection assembly. In this embodiment, the liquid pump 102 directly discharges the waste liquid collected in the premixing tank 103 and / or the reaction tank 104 into the external waste liquid collection assembly 119. The liquid pump 102 provides greater power, which can reduce the problem of overflow caused by waste liquid not being cleaned in time in the premixing tank 103 and / or the reaction tank 104.

[0084] Combination Figure 4 , Figure 4 A schematic diagram of the liquid circuit structure of a blood cell analyzer according to an embodiment of the present invention is shown.

[0085] like Figure 4As shown, this application provides a blood cell analyzer, including: a premixing cell 103, a sheath flow impedance detection component 105, a reaction cell 104, and an optical detection component 106. The premixing cell 103 is connected to the sheath flow impedance detection component 105, and the reaction cell 104 is connected to the optical detection component 106; a pressure source is connected to the sheath flow impedance detection component 105 and the optical detection component 106. The system includes: a premixing tank 103 for preparing a first sample solution; a sheath flow impedance detection component 105 for detecting the first sample solution to obtain first detection data; the sheath flow impedance detection component 105 is connected to the premixing tank 103, and the premixing tank 103 is also used to receive a first waste liquid generated during the sample detection process of the sheath flow impedance detection component 105 on the first sample solution; a reaction tank 104 for preparing a second sample solution; an optical detection component 106 for detecting the second sample solution to obtain second detection data; the optical detection component 106 is connected to the reaction tank 104, and the reaction tank 104 is also used to receive a second waste liquid generated during the sample detection process of the optical detection component 106 on the second sample solution; the optical detection component 106 is different from the sheath flow impedance detection component 105; and a pressure source connected to the premixing tank 103 and the reaction tank 104, the pressure source being used to discharge the first waste liquid in the premixing tank 103 and / or the second waste liquid in the reaction tank 104 from the blood cell analyzer. The blood cell analyzer performs the waste liquid treatment method described in any of the above embodiments at least through the premixing tank 103 and the sheath flow impedance detection component 105, and / or the reaction tank 104 and the optical detection component 106.

[0086] The waste liquid treatment method and the blood cell analyzer using this method provided in this application discharge the waste liquid from the sheath current impedance detection component 105 into the premixing tank 103 and / or the waste liquid from the optical detection component 106 into the reaction tank 104. Compared with the existing blood cell analyzers that require two waste liquid tanks, this method does not require the addition of an independent waste liquid tank for waste liquid treatment. It can reduce the size of the blood cell analyzer and reduce the manufacturing cost of the blood cell analyzer. The blood cell analyzer has a smaller overall size, lower cost, higher waste liquid treatment efficiency, and higher overall detection efficiency, and has a higher cost performance.

[0087] In the description of this application, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0088] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0089] The above are merely embodiments of this application and do not limit the scope of this patent application. Any equivalent structural or procedural changes made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the scope of patent protection of this application.

Claims

1. A liquid circuit structure for a blood cell analyzer, characterized in that, The device includes a premixing cell, a reaction cell, a sample preparation component, a sample solution propulsion component, a sheath flow impedance detection component, and an optical detection component. The sample preparation component connects the premixing cell and the reaction cell. The sample solution propulsion component connects the sheath flow impedance detection component and the optical detection component. The premixing cell and the reaction cell share a single sample preparation component. The premixing cell is used to prepare a first sample solution, and the reaction cell is used to prepare a second sample solution. The sheath flow impedance detection component and the optical detection component share a single sample solution propulsion component. The sheath flow impedance detection component is used for red blood cell counting, and the optical detection component is used for white blood cell differential detection. The liquid circuit structure also includes multiple liquid tubes. Before the first sample liquid and the second sample liquid are tested, the sample preparation component draws the first sample liquid from the premixing tank into the liquid tube between the premixing tank and the sample preparation component. The sample preparation component draws the second sample liquid from the reaction tank into the liquid tube between the reaction tank and the sample preparation component. The sample liquid pushing component pushes the first sample liquid in the liquid tube between the premixing cell and the sample preparation component into the sheath flow impedance detection component for sample detection; the sample liquid pushing component pushes the second sample liquid in the liquid tube between the reaction cell and the sample preparation component into the optical detection component for sample detection. The premixing cell receives the preparation waste liquid generated during the preparation of the first sample liquid and the second sample liquid, as well as the first waste liquid generated during the sample detection of the first sample liquid by the sheath flow impedance detection component. The reaction cell receives the second waste liquid generated during the sample detection of the second sample liquid by the optical detection component.

2. The fluid circuit structure according to claim 1, characterized in that, The liquid circuit structure also includes: a sixth liquid pipe, a seventh liquid pipe, an eighth liquid pipe, a ninth liquid pipe, a sheath flow impedance sample preparation valve, a first on / off element, a second liquid pipe, a third liquid pipe, and a fourth liquid pipe; The sample preparation component is connected to one end of the eighth liquid tube, the other end of the eighth liquid tube is connected to one end of the ninth liquid tube, the other end of the ninth liquid tube is connected to one end of the sixth liquid tube, the other end of the sixth liquid tube is connected to the premixing cell, one end of the sixth liquid tube is connected to one end of the sheath flow impedance sample preparation valve, the other end of the sheath flow impedance sample preparation valve is connected to one end of the seventh liquid tube, and the other end of the seventh liquid tube is connected to the sample preparation component. The reaction tank is connected to one end of the second liquid pipe, one end of the second liquid pipe is connected to one end of the first switch, the other end of the first switch is connected to one end of the third liquid pipe, the other end of the third liquid pipe is connected to one end of the fourth liquid pipe, and the other end of the fourth liquid pipe is connected to the sample preparation component. By opening the sheath flow impedance sample preparation valve, the sample preparation component provides negative pressure, allowing the first sample solution prepared in the premixing cell to enter the seventh liquid tube via the sheath flow impedance sample preparation valve, so as to complete the preparation of the first sample solution before sample detection. By opening the first switch, the sample preparation component provides negative pressure, and the second sample liquid prepared in the reaction cell enters the third liquid tube sequentially through the second liquid tube and the first switch, so as to complete the preparation of the second sample liquid before sample detection. During the process of the sample preparation component drawing up the first sample liquid and the second sample liquid for testing, the waste liquid generated when the sample preparation component draws up the first sample liquid and the second sample liquid enters the premixing tank sequentially through the eighth liquid tube, the ninth liquid tube and the sixth liquid tube.

3. The fluid circuit structure according to claim 2, characterized in that, It also includes a first liquid tube, the sample preparation component is connected to the reaction cell through the first switch; the liquid inlet of the optical detection component is connected to the first switch, the liquid outlet of the optical detection component is connected to one end of the first liquid tube, and the other end of the first liquid tube is connected to the reaction cell. By turning on the first switch, the optical detection component detects the second sample liquid, and the sample liquid pushing component pushes the second waste liquid generated during the detection of the second sample liquid by the optical detection component into the first liquid tube; By closing the first on / off element, the sample liquid pushing assembly injects the second waste liquid in the first liquid tube into the reaction tank.

4. The fluid passage structure according to claim 3, characterized in that, The length of the first liquid tube is less than or equal to 60 mm.

5. The fluid circuit structure according to claim 1, characterized in that, The blood cell analyzer also includes a dilution tank, a positive pressure source, a second switch, a third switch, and a fourth switch. The diluent pool is connected to the positive pressure source and the second switching element; the second switching element is connected to the third switching element; the third switching element is connected to the sheath current impedance detection component; the second switching element is connected to the fourth switching element; and the fourth switching element is connected to the optical detection component. By opening the second and third switching devices, the diluent in the diluent pool is injected into the sheath flow impedance detection component via the second and third switching devices, and a stable sheath flow is formed in the sheath flow impedance detection component. By opening the second and fourth switches, the diluent in the diluent pool is injected into the optical detection assembly via the second and fourth switches, forming a stable sheath flow in the optical detection assembly.

6. The fluid circuit structure according to claim 5, characterized in that, The sheath flow impedance detection assembly includes a sheath flow impedance detection cell, and the sheath flow impedance detection cell includes a fore chamber; the liquid circuit structure further includes: a fifth switching element; the fifth switching element connects the fore chamber and one end of the eighth liquid pipe; By opening the second and fifth switching devices, the positive pressure source is activated. During the process of the sheath flow impedance detection cell performing sample detection on the first sample liquid, the first waste liquid generated is introduced into the premixing cell from the front cell via the fifth switching device, the eighth liquid pipe, the ninth liquid pipe, and the sixth liquid pipe under the action of the positive pressure and the sample liquid pushing component.

7. The fluid circuit structure according to claim 6, characterized in that, The sheath current impedance detection cell further includes a rear cell, and the front cell and the rear cell are connected through a sapphire hole. The liquid circuit structure further includes a sixth switch; the sixth switch connects the rear cell and the second switch, and the rear cell is connected to one end of the ninth liquid pipe. By opening the second and sixth switching devices, the positive pressure source is activated. During the process of the sheath flow impedance detection cell performing sample detection on the first sample liquid, the first waste liquid generated is introduced into the premixing cell from the rear cell through the ninth and sixth liquid pipes under positive pressure and the sample liquid pushing component.

8. The fluid circuit structure according to claim 1, characterized in that, It also includes a pressure source, through which the first waste liquid in the premixing tank and the second waste liquid in the reaction tank are discharged from the blood cell analyzer.

9. The fluid circuit structure according to claim 8, characterized in that, The pressure source includes a liquid pump, which is connected to the premixing tank, the reaction tank, and an external waste liquid collection assembly located outside the blood cell analyzer. By turning on the liquid pump, the first waste liquid in the premixing tank and / or the second waste liquid in the reaction tank are directly discharged from the blood cell analyzer, so that the first waste liquid and / or the second waste liquid are injected into the external waste liquid collection assembly.

10. A blood cell analyzer, characterized in that, Includes the fluid circuit structure as described in any one of claims 1-9.

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