A trans- caryophyllene based aphis gossypii repellent

Abstract

The present application relates to the technical field of pest green prevention and control, and particularly relates to a kind of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of aphid of a kind of aphid of aphid of aphid of aphid of aphid of aphideffective component includes trans-caryophyllene.The present application finds that trans-caryophyllene has good repellent effect on aphid, and is prepared into aphid repellent by using trans-caryophyllene, which belongs to the use of chemical information material to repel pests.The

Description

[0001] This application is a divisional application of the invention patent application filed on December 29, 2023, with application number 202311864974.X, entitled "A Spiraea aphid repellent and its preparation method and application". Technical Field

[0002] This invention relates to the field of green pest control technology, and in particular to a repellent for Spiraea aphids based on trans-caryophyllene. Background Technology

[0003] Spiraea aphid ( Aphis Spiraecola Patch (Aphididae, genus Aphididae, order Hemiptera) is a major pest of fruit trees in orchards in northern my country, primarily affecting apples, pears, and meadowsweet. Adults and nymphs congregate to damage new shoots, buds, and leaves, sucking sap. Infested leaves curl horizontally towards the underside, hindering shoot growth and, in severe cases, weakening the tree. Chemical methods are commonly used for control. However, the extensive use of chemical pesticides has significantly reduced the types and numbers of natural enemies, led to pesticide resistance in pests, resulted in severe pesticide residues, and caused serious environmental pollution.

[0004] Plant-derived attractants and repellents fall under the category of chemical ecological regulation and can be composed of single or multiple plant secondary metabolites and their derivatives. Identifying the composition and structure of these substances can elucidate the regulatory mechanisms of important plant secondary metabolites on insect behavior. Furthermore, these highly effective plant secondary metabolites can be developed into attractant or repellent products for pest control. These products are environmentally friendly, residue-free, and safe and non-toxic to humans and animals, and have broad application prospects under the current concept of green and sustainable agricultural development.

[0005] Therefore, developing and utilizing attractants / repellents to control Spiraea aphids can not only replace highly toxic chemical pesticides to a certain extent, but also reduce the amount of chemical pesticides used, and further solve problems such as pest resistance, environmental pollution, and excessive pesticide residues.

[0006] In conclusion, developing "green pest control" strategies has become the primary issue in the field of new plant protection technology research. Summary of the Invention

[0007] Based on the above, the present invention provides a repellent for Spiraea aphids, its preparation method, and its application.

[0008] To achieve the above objectives, the present invention provides the following solution: One of the technical solutions of this invention is a Spiraea aphid repellent, the active ingredients of which include at least one of cis-citral, nepetalactone, trans-caryophyllene, β-pinene, and eugenol.

[0009] In a preferred embodiment of the present invention, the volume concentration of the active ingredient in the spirea aphid repellent is 20-50%.

[0010] The second technical solution of the present invention is a method for preparing the above-mentioned Spiraea aphid repellent, wherein the active ingredient is mixed evenly with a diluent to obtain the Spiraea aphid repellent.

[0011] In a preferred embodiment of the present invention, the diluent is liquid paraffin.

[0012] The third technical solution of this invention is the application of the above-mentioned Spiraea aphid repellent in the preparation of Spiraea aphid repellent drugs.

[0013] The fourth technical solution of the present invention is the application of the above-mentioned cis-citral, nepetalactone, trans-caryophyllene, β-pinene and eugenol in repelling Spiraea aphids.

[0014] The fifth technical solution of the present invention is the application of the above-mentioned cis-citral, nepeta lactone, trans-caryophyllene, β-pinene, and eugenol in the preparation of aspirin aphid repellent.

[0015] The sixth technical solution of the present invention is a method for improving the repellency effect of Spiraea japonica aphids. This method improves the repellency effect of Spiraea japonica aphids by adding at least one of the above-mentioned cis-citral, nepetalactone, trans-caryophyllene, β-pinene, and eugenol to the drug that repels Spiraea japonica aphids.

[0016] The present invention discloses the following technical effects: This invention discovers that cis-citral, nepetalactone, trans-caryophyllene, β-pinene, and eugenol have excellent repellent effects against Spiraea japonica aphids; and utilizes these to prepare a Spiraea japonica aphid repellent, belonging to the category of using chemical signaling substances to repel pests. This Spiraea japonica aphid repellent can be hung in orchards to repel Spiraea japonica aphids and reduce their damage. Furthermore, this Spiraea japonica aphid repellent is environmentally friendly, reduces the use of chemical pesticides, and is particularly suitable for widespread application in orchards, possessing good economic, social, and ecological benefits and broad industrialization prospects. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 The repellency of the Spiraea aphids by the Spiraea aphid repellents prepared in Examples 1-5 and Comparative Example 1 is shown. Detailed Implementation

[0019] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0020] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0021] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0022] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0023] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0024] The first aspect of this invention provides a repellent for Spiraea aphids, the active ingredient of which includes at least one of cis-citral, nepetalactone, trans-caryophyllene, β-pinene, and eugenol.

[0025] In a preferred embodiment of the present invention, the volume concentration of the active ingredient in the spirea aphid repellent is 20-50%.

[0026] A second aspect of the present invention provides a method for preparing the above-mentioned Spiraea aphid repellent, wherein the active ingredient is mixed evenly with a diluent to obtain the Spiraea aphid repellent.

[0027] In a preferred embodiment of the present invention, the diluent is liquid paraffin.

[0028] The above-mentioned Spiraea aphid repellent has a significant repellency against Spiraea aphids and can be used in orchards to control Spiraea aphids.

[0029] The third aspect of this invention provides the application of the above-mentioned Spiraea aphid repellent in the preparation of Spiraea aphid repellent drugs.

[0030] In a preferred embodiment of the present invention, the application method is as follows: the spirea aphid repellent is added to the slow-release device and fixed on a yellow board. During the peak summer aphid occurrence period (June-September) in the orchard, the device is hung in the orchard area to repel spirea aphids and reduce damage.

[0031] The slow-release device can be prepared by the following steps: make two small holes in the centrifuge tube with wire, and plug the mouth of the bottle with degreased cotton without immersing it in liquid.

[0032] The fourth aspect of the present invention provides the application of the above-mentioned cis-citral, nepetalactone, trans-caryophyllene, β-pinene, and eugenol in repelling Spiraea aphids.

[0033] The fifth aspect of the present invention provides the application of the above-mentioned cis-citral, nepetalactone, trans-caryophyllene, β-pinene, and eugenol in the preparation of a Spiraea aphid repellent.

[0034] The sixth aspect of the present invention provides a method for improving the repellency effect of Spiraea zebrina aphids, which improves the repellency effect by adding at least one of the above-mentioned cis-citral, nepetalactone, trans-caryophyllene, β-pinene, and eugenol to a drug that repels Spiraea zebrina aphids.

[0035] Unless otherwise specified, all raw materials used in the embodiments of this invention can be obtained through commercial channels.

[0036] The present invention will be further illustrated by the following examples.

[0037] Example 1 Add 30 μL of cis-citral to 120 μL of liquid paraffin, shake well, place in a slow-release device, and fix on a yellow board to obtain the Spiraea aphid repellent.

[0038] Example 2 Add 30 μL of nepetalactone to 120 μL of liquid paraffin, shake well, place in a slow-release device, and fix on a yellow board to obtain the Spiraea aphid repellent.

[0039] Example 3 Add 30 μL of trans-caryophyllene to 120 μL of liquid paraffin, shake well, place in a slow-release device, and fix on a yellow board to obtain the Spiraea aphid repellent.

[0040] Example 4 Add 30 μL of β-pinene to 120 μL of liquid paraffin, shake well, place in a slow-release device, and fix on a yellow board to obtain the Spiraea aphid repellent.

[0041] Example 5 Add 30 μL of eugenol to 120 μL of liquid paraffin, shake well, place in a slow-release device, and fix on a yellow board to obtain the Spiraea aphid repellent.

[0042] Comparative Example 1 Take 150 μL of liquid paraffin, place it in a slow-release device and fix it on a yellow plate to obtain the control treatment.

[0043] Spirea repellents prepared in Examples 1, 2, 3, 4, and 5, along with the control treatment prepared in Comparative Example 1, were hung in an apple orchard in Tumushuk City, Third Division of Xinjiang Production and Construction Corps. Each treatment was replicated five times, with a spacing of four fruit trees between treatments. The hanging height was approximately 1.5 meters, and the hanging time was in early August. The number of Spirea aphids on the yellow sticky boards was counted three days later. Data analysis was performed using one-way ANOVA and Duncan's multiple range test. The results are shown in Table 1 and... Figure 1 As shown.

[0044] From Table 1 and Figure 1 The survey data shows that the repellent described in this invention has a significant repellent effect on Spiraea aphids.

[0045] Table 1: Repellent effect of various implementation examples on Spiraea aphids in apple orchards (2023) Note: The number of Spiraea aphids is an average value, and different letters represent significant differences at the 0.05 level.

[0046] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A repellent for Spiraea aphids, characterized in that, The active ingredient includes trans caryophyllene.

2. The spirea aphid repellent according to claim 1, characterized in that, The volume concentration of the active ingredient in the Spiraea aphid repellent is 20-50%.

3. A method for preparing the spirea aphid repellent according to claim 1 or 2, characterized in that, The active ingredient is mixed evenly with the diluent to obtain the Spiraea aphid repellent.

4. The method for preparing the Spiraea aphid repellent according to claim 3, characterized in that, The diluent is liquid paraffin.

5. The application of the Spiraea aphid repellent as described in claim 1 or 2 in the preparation of Spiraea aphid repellent products.

6. A method for improving the repellency effect of spirea aphids, characterized in that, The repellency of Spiraea aphids can be improved by adding trans-caryophyllene to the drug.

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