A root maggot larva feed and a method of preparing the same

Abstract

The present application relates to the field of feed, in particular to a kind of root maggot larva feed and preparation method thereof.The root maggot larva feed is composed of the following raw materials: beer yeast powder, wheat germ powder, garlic leaf powder, vitamin C, linoleic acid, agar, nipagin, sorbic acid and water.The root maggot larva feed prepared by the present application contains various nutrients required by larvae, so that the feed can be nutritionally balanced, thereby ensuring the physiological state of larvae to be stable and consistent and improving the survival rate, pupation rate and eclosion rate of root maggot larvae;the mold resistance is improved, and the shelf life of the feed is prolonged.The preparation method of the root maggot larva feed of the present application is simple to operate, suitable for large-scale production, meets the needs of scale-up, standardization and long-term subculturing of root maggot larvae, and provides reliable support for carrying out related scientific research.

Description

Technical Field

[0001] This invention relates to the field of insect feed, specifically to a root maggot larvae feed and its preparation method. Background Technology

[0002] Root maggots are a serious underground pest that can damage various crops such as garlic, leeks, and scallions, causing reduced yields and lower quality, resulting in significant economic losses for growers. To obtain better control methods, research on the biological characteristics of root maggots, their resistance mechanisms, and the screening of biological control resources has received considerable attention. Related studies all require a large number of uniformly developed insects in consistent physiological states; large-scale, standardized rearing techniques are a crucial prerequisite for conducting such research, directly affecting the accuracy and reliability of the experimental results.

[0003] However, root maggots are underground borers with specific feeding habits, requiring high levels of nutrition, texture, and odor in their feed. Furthermore, they require a high-humidity environment for rearing, which easily leads to mold growth and contamination by other microorganisms, resulting in low larval survival rates and uneven development. Currently, indoor rearing of root maggots mainly uses natural feed and traditional artificial feed. Natural feed shows obvious mold colonies and soft, moldy tissue after only 3-5 days, requiring frequent replacement, leading to cumbersome operations, high costs, and easy breeding of other microorganisms, as well as low larval survival rates. This method is only suitable for small-scale, short-term rearing. Conventional artificial feed suffers from problems such as easy mold growth in high-humidity environments and unreasonable nutritional ratios. Additionally, some formulas contain high-cost ingredients or antibiotics, affecting the physiological state of the test insects and failing to meet the needs of large-scale, standardized rearing and precise scientific research. Therefore, there is an urgent need to develop a new type of artificial feed that is resistant to mold, nutritionally balanced, and low-cost, as well as supporting feeding technology. This feed should be able to adapt to high-humidity feeding environments, extend the service life, improve the survival rate and uniformity of larvae development, ensure the stability of the physiological state of the test insects, and meet the needs of large-scale, standardized, and long-term breeding of garlic root maggots. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a root maggot larvae feed and its preparation method.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: The first aspect of the present invention provides a root maggot larvae feed, which is prepared from the following raw materials in the following proportions: 35-40 g brewer's yeast powder, 30-40 g wheat germ powder, 150-200 g garlic leaf powder, 5-10 g vitamin C, 500 µL linoleic acid, 15-20 g agar, and 1 L water.

[0006] Furthermore, the root maggot is the leek maggot.

[0007] The second aspect of the present invention provides a root maggot larvae feed, which is prepared from the following raw materials in the following proportions: 35-40 g brewer's yeast powder, 30-40 g wheat germ powder, 150-200 g garlic leaf powder, 5-10 g vitamin C, 500 µL linoleic acid, 15-20 g agar, 3-8 g parabens, 5-10 g sorbic acid, and 1 L water.

[0008] Furthermore, the root maggot is the leek maggot.

[0009] A third aspect of the present invention provides a method for preparing root maggot larvae feed as described in the first and second aspects above, comprising the following steps: (1) Weigh out brewer's yeast powder, wheat germ powder and garlic leaf powder and add them to 300 mL to 400 mL of water. Mix well to obtain component A; (2) Add agar to the remaining water and boil to obtain component B; (3) Add component B to component A and stir until homogeneous. After cooling, add the remaining raw materials and mix well to obtain the final product.

[0010] Furthermore, in step (3), the temperature is cooled to below 45 °C before the remaining raw materials are added.

[0011] Furthermore, the prepared root maggot larvae feed was stored in a refrigerator at 4 ℃.

[0012] Compared with the prior art, the positive and beneficial effects achieved by the present invention are as follows: (1) The wheat germ powder used in this invention provides the necessary carbohydrates, proteins, fatty acids, vitamins and dietary fiber for root maggot larvae; promotes the growth of root maggot larvae and effectively improves their survival rate; balances nutrition and enables root maggot larvae to develop stably; and at the same time improves the palatability of the feed and reduces costs.

[0013] (2) Linoleic acid is a fatty acid required for the physiology of root maggot larvae. The addition of linoleic acid to the feed prepared in this application improves the survival rate and uniformity of root maggot larvae development, which is conducive to large-scale breeding and thus ensures the pupation rate and emergence rate of root maggot larvae. At the same time, it increases the content of unsaturated fatty acids in the insect body, improves the vitality of larvae, and makes it conducive to the research and development of root maggot control technology.

[0014] (3) The added garlic leaf powder contains active sulfides and allicin, which inhibit harmful bacteria in the feed and the insect's intestines. Combined with the added preservatives parabens and sorbic acid, it improves the feed's resistance to mold and extends its shelf life. The garlic aroma in the garlic leaf powder enhances the palatability of the feed. At the same time, it improves the digestive function of larvae, increases the absorption efficiency of protein and carbohydrates, and makes the insects more uniform. In addition, garlic leaf powder can reduce the putrid odor of the feed, reduce the breeding of flying insects, and optimize the breeding environment.

[0015] (4) The brewer's yeast powder used in this invention is rich in protein, vitamins, minerals and trace elements required for the growth and development of root maggot larvae, which can ensure that the larvae can successfully complete the pupation and emergence process, while supporting the normal egg laying and hatching of adults; it improves the palatability of the feed; it works synergistically with agar to improve the texture of the feed, effectively prevents stratification and extends the shelf life; at the same time, brewer's yeast powder can inhibit miscellaneous bacteria and reduce mold.

[0016] (5) The root maggot larvae feed prepared by the present invention contains various nutrients required by the larvae, so that the feed can be nutritionally balanced, thereby ensuring the stable and consistent physiological state of the larvae and improving the survival rate, pupation rate and eclosion rate of root maggot larvae; improving the resistance to mold and extending the shelf life of the feed; and the raw materials are readily available, reducing the feeding cost.

[0017] (6) The method for preparing root maggot larvae feed of the present invention is simple to operate, suitable for large-scale production, meets the needs of large-scale, standardized and long-term breeding of root maggot larvae, and provides reliable support for carrying out related scientific research. Detailed Implementation

[0018] The present invention will be further described below through specific embodiments. These embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention. Example 1

[0019] Sample 1 and Sample 2 were prepared according to the following raw material composition and preparation method. The specific amount of each raw material is shown in Table 1.

[0020] A feed for root maggot larvae is composed of the following ingredients: brewer's yeast powder, wheat germ powder, garlic leaf powder, vitamin C, linoleic acid, agar, and water.

[0021] The method for preparing this root maggot larvae feed includes the following steps: (1) Add brewer's yeast powder, wheat germ powder and garlic leaf powder to 400 mL of water and mix well to obtain component A; (2) Add agar to 600 mL of water and boil to obtain component B; (3) Add component B to component A, cool to below 45 °C, add vitamin C and linoleic acid, mix well, and you will get the root maggot larvae feed. Store it in a refrigerator at 4 °C. Example 2

[0022] Samples 3 and 4 were prepared according to the following raw material composition and preparation method. The specific amount of each raw material is shown in Table 1.

[0023] A feed for root maggot larvae is composed of the following ingredients: brewer's yeast powder, wheat germ powder, garlic leaf powder, vitamin C, linoleic acid, agar, parabens, sorbic acid, and water.

[0024] The method for preparing this root maggot larvae feed includes the following steps: (1) Add brewer's yeast powder, wheat germ powder and garlic leaf powder to 300 mL of water and mix well to obtain component A; (2) Add agar to 700 mL of water and boil to obtain component B; (3) Add component B to component A, cool to below 45 °C, add vitamin C, linoleic acid, parabens and sorbic acid, mix well, and you will get the root maggot larvae feed. Store it in a refrigerator at 4 °C.

[0025] Comparative Example 1 Comparative sample 1 consists of the following ingredients: brewer's yeast powder, garlic leaf powder, vitamin C, linoleic acid, agar, parabens, sorbic acid, and water. The specific amounts of each ingredient are shown in Table 1.

[0026] The preparation method of Comparative Sample 1 includes the following steps: (1) Add brewer's yeast powder and garlic leaf powder to 400 mL of water and mix well to obtain component A; (2) Add agar to 600 mL of water and boil to obtain component B; (3) Add component B to component A, cool, add vitamin C, linoleic acid, parabens and sorbic acid, mix well to obtain control sample 1, and store in a refrigerator at 4 ℃.

[0027] Comparative Example 2 Comparative sample 2 consists of the following ingredients: brewer's yeast powder, wheat germ powder, garlic leaf powder, vitamin C, agar, parabens, sorbic acid, and water. The specific amounts of each ingredient are shown in Table 1.

[0028] The preparation method of comparative sample 2 includes the following steps: (1) Add brewer's yeast powder, wheat germ powder and garlic leaf powder to 400 mL of water and mix well to obtain component A; (2) Add agar to 600 mL of water and boil to obtain component B; (3) Add component B to component A, cool, add vitamin C, parabens and sorbic acid, mix well, and you will get control sample 2. Store it in a refrigerator at 4 ℃.

[0029] Comparative Example 3 Comparative sample 3 was composed of the following ingredients: brewer's yeast powder, wheat germ powder, vitamin C, linoleic acid, agar, parabens, sorbic acid, and water. The specific amounts of each ingredient are shown in Table 1.

[0030] The preparation method of comparative sample 3 includes the following steps: (1) Add brewer's yeast powder and wheat germ powder to 400 mL of water and mix well to obtain component A; (2) Add agar to 600 mL of water and boil to obtain component B; (3) Add component B to component A, cool, add vitamin C, linoleic acid, parabens and sorbic acid, mix well to obtain control sample 3, and store in a refrigerator at 4 ℃.

[0031] The proportions of each raw material used in samples 1-4 and control samples 1-3 are shown in Table 1:

[0032] Experimental Example 1 Under conditions of 25–26 °C and 40–50% relative humidity, the appearance of mold spots on samples 1–4 and control samples 1–3 was observed to test their resistance to mold. Samples 1 and 2 developed mold spots within 6–7 days; samples 3 and 4, and control samples 1–3 showed no mold spots after 90 days. Furthermore, samples 3 and 4, and control samples 1–3, stored in a refrigerator at 2–5 °C, showed no mold spots after 12 months. This indicates that the root maggot larvae feed with added preservatives (parabens, sorbic acid) exhibits better resistance to mold than the unpreserved feed. This means that the unpreserved feed requires frequent, small-volume additions during the rearing period, while the preservative-added feed eliminates the need for frequent changes, reducing workload. It also allows for continuous feeding of root maggot larvae throughout their entire lifespan, supporting their larval development, and prevents significant mold growth or concentrated outbreaks of other microorganisms during the rearing period. This demonstrates that the root maggot larvae feed prepared by this invention can effectively solve many defects in existing breeding techniques, and its long shelf life can meet the needs of product circulation, as well as the needs of large-scale, standardized, long-term subspecies breeding of root maggot larvae and the industrialization and scientific research needs of scientific research experiments.

[0033] Experiment Example 2 Under the same conditions, leek maggots were fed natural feed (garlic leaves) and samples 3, 4, and control samples 1-3. The feeding effect was observed. The experimental method is as follows.

[0034] I. Experimental Methods 1. Obtaining experimental larvae Take multiple pairs of mature male and female pupae and place them in a rearing box. Add 25 g of maggot feed to each rearing box and wait for the male and female pupae to emerge and lay eggs. Collect the second-instar larvae on the 4th to 5th day after egg laying.

[0035] 2. Survival rate and pupation rate statistics Take 300 second-instar larvae as a group and place them in the same rearing box. Add 25 g of maggot feed to each rearing box and set the temperature to 25-26 ℃ and the relative humidity to 40-50% until the pupal stage. Each feed sample is a treatment and is repeated 4 times.

[0036] During the feeding process, the number of surviving leek maggot larvae was counted every 3 days, and the survival rate was calculated.

[0037] During the pupal stage, observations were conducted in three time periods within 24 hours each day to collect pupae in a timely manner, keep records, and calculate the pupation rate.

[0038] 3. Statistics on emergence rate and number of eggs laid per female The pupae collected from each rearing box were placed in an environment with a set temperature of 25-26 ℃ and a relative humidity of 40-50% for cultivation. The pupae that emerged normally and completely were observed and counted daily until the end of the emergence period, and the emergence rate was calculated.

[0039] After the adult insects emerged from each rearing box, the samples were mixed together. Fifty pairs of male and female adults were randomly selected, and each pair was placed in a culture box with a diameter of 13 cm and a height of 13.5 cm to lay eggs. Moistened filter paper and small cotton balls soaked in 10% honey water were placed at the bottom of the culture box. After 3 days, the eggs were picked out and counted with a brush. The count was maintained daily until the female insect died. The total number of eggs laid by a single female is the egg production rate.

[0040] II. Experimental Results Table 2 lists the statistical results of survival rate, pupation rate, eclosion rate, and number of eggs laid per female after feeding leek maggots with different feeds.

[0041]

[0042] As shown in Table 2, the survival rate, pupation rate, eclosion rate and single female egg production of the leek maggots fed with the feed prepared by Samples 3 and 4 were very close to those of the natural feed group, and some indicators were even better. However, the natural feed rotted and smelled bad after 4 days of continuous use and needed to be replaced every day. In addition, the natural feed was significantly affected by the season and could not be supplied stably all year round.

[0043] The feeds prepared from samples 3 and 4 showed better survival rates, pupation rates, emergence rates, and egg production per female compared to controls 1-3. This indicates that adding wheat germ powder, linoleic acid, and garlic leaf powder to the feed not only provides various nutrients for leek maggot larvae but also improves their survival rate, enabling stable development and ensuring uniform development, thereby increasing pupation and emergence rates. Simultaneously, it reduces feeding costs, offering higher cost-effectiveness and meeting the needs of large-scale, standardized, and long-term breeding of leek maggot larvae, providing reliable support for related scientific research.

[0044] The present invention has been described above by way of example. It should be noted that any simple modifications, alterations or other equivalent substitutions that can be made by those skilled in the art without creative effort without departing from the core of the present invention fall within the scope of protection of the present invention.

Claims

1. A feed for root maggot larvae, characterized in that, The root maggot larvae feed is prepared from the following ingredients in the following proportions: 35-40 g brewer's yeast powder, 30-40 g wheat germ powder, 150-200 g garlic leaf powder, 5-10 g vitamin C, 500 µL linoleic acid, 15-20 g agar, and 1 L water.

2. A feed for root maggot larvae, characterized in that, The root maggot larvae feed is prepared from the following ingredients in the following proportions: 35-40 g brewer's yeast powder, 30-40 g wheat germ powder, 150-200 g garlic leaf powder, 5-10 g vitamin C, 500 µL linoleic acid, 15-20 g agar, 3-8 g parabens, 5-10 g sorbic acid, and 1 L water.

3. The root maggot larvae feed as described in claim 1 or 2, characterized in that, The root maggots are leek maggots.

4. The method for preparing root maggot larvae feed as described in claim 1 or 2, characterized in that, Includes the following steps: (1) Weigh out brewer's yeast powder, wheat germ powder and garlic leaf powder and add them to 300 mL to 400 mL of water. Mix well to obtain component A; (2) Add agar to the remaining water and boil to obtain component B; (3) Add component B to component A and stir until homogeneous. After cooling, add the remaining raw materials and mix well to obtain the final product.

5. The method for preparing root maggot larvae feed as described in claim 4, characterized in that, In step (3), the temperature is cooled to below 45°C before the remaining raw materials are added.

6. The method for preparing root maggot larvae feed as described in claim 4, characterized in that, The prepared root maggot larvae feed was stored in a refrigerator at 4 ℃.

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