A mold and sectioning method for making tree shrew TTC-stained brain sections

Abstract

The application discloses a mold for preparing a tree shrew TTC-stained brain section and a sectioning method, and relates to the field of sectioning tools. The mold for preparing a tree shrew TTC-stained brain section and the sectioning method are characterized by being provided with a blade, a placing groove and a knife groove. When a tree shrew brain section is prepared, the tree shrew brain block can be placed in the placing groove, then the pressing plate is pressed to drive the blade to be inserted into the knife groove, the tree shrew brain block is cut into sections by the blade, and the cut tree shrew brain block is stably dropped in the placing groove. The whole device is simple to operate, the shape of the tree shrew brain section is not affected, and the tree shrew brain section is convenient for an operator to take.

Description

Technical Field

[0001] This invention relates to slicing tool technology, specifically to a mold and slicing method for preparing TTC-stained brain slices from tree shrews. Background Technology

[0002] Tree shrews are small mammals resembling squirrels with relatively long snouts. Taxonomically, they belong to the order Plasmacytota, which lies between primates and insectivores. They are mainly distributed in South Asia, Southeast Asia, and southern my country. There are 12 species and 50 subspecies of tree shrews worldwide, with 1 species and 6 subspecies found in China. The Yunnan tree shrew, from China, is the most widely used species for life science research by scholars worldwide. The tree shrew's genome is 93.4% similar to that of primates, and its anatomy, physiology, biochemistry, and immunology are similar to those of humans, making it a promising candidate for biomedical research.

[0003] Chinese patent CN203198001U mentions a tree shrew whole-brain slicing mold, belonging to the field of life science devices. A recessed portion conforming to the shape of the ventral surface of the tree shrew's whole brain is provided on a corrosion-resistant metal block. Multiple equally spaced cutting guide grooves, 1.5–2.5 mm deeper than the lowest point of the recess, are provided vertically downwards from the upper surface of the metal block, either longitudinally or laterally. If the cutting guide grooves are distributed laterally, an additional cutting guide groove coinciding with the midline of the left and right hemispheres of the tree shrew's brain is also provided. Two recessed portions are provided at the bottom of the recess corresponding to the two occipital lobes of the tree shrew's brain, and two extension portions are provided at the front of the recess corresponding to the two olfactory bulbs of the tree shrew's brain. This slicing mold allows for convenient and rapid slicing of the tree shrew's whole brain without causing compression deformation or damage to brain tissue.

[0004] During the application process, the applicant discovered that the above-mentioned application has defects in use. The slicing mold disclosed in the application consists only of a cutter and a cutter guide groove. The whole brain of the tree shrew will move inside the recess, which is not convenient for the cutter to slice. Moreover, the sliced ​​whole brain of the tree shrew will stick to the surface of the cutter. If multiple cutters are used, the sliced ​​head of the tree shrew may even get stuck in the gap between the slices, which is not conducive to the operator taking the sliced ​​brain of the tree shrew. Summary of the Invention

[0005] The purpose of this invention is to provide a mold and slicing method for making TTC-stained brain slices of tree shrews, so as to solve the problem that the existing tree shrew brain slice molds are not convenient for slicing tree shrew brain slices.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a mold for preparing TTC-stained brain slices from tree shrews, comprising:

[0007] The mounting housing has multiple cutting grooves on its top to form a cutting area, and a placement groove that communicates with the cutting grooves is formed on the upper surface of the mounting housing in the cutting area.

[0008] A telescopic rod is slidably connected to the mounting housing, and a pressure plate is hinged to one side of the telescopic rod. A blade is connected to the pressure plate, and the blade is arranged opposite to the blade groove.

[0009] Preferably, the mounting shell has a telescopic hole, the telescopic rod is inserted into the telescopic hole, and a spring is provided in the telescopic hole.

[0010] Preferably, the placement slot is elliptical in shape.

[0011] Preferably, the blade is slidably engaged with the pressure plate.

[0012] A method for preparing TTC-stained brain slices from tree shrews, comprising the following steps:

[0013] Place the tree shrew brain block in the placement slot;

[0014] Rotate the pressure plate so that the blade edge abuts against the tree shrew brain block, and press the pressure plate to drive the telescopic rod to compress the spring. Multiple pressure plates are interlocked in the blade groove to slice the tree shrew brain block.

[0015] Release the pressure plate, the compressed spring returns to its original position and pushes the telescopic rod upward, lifting the pressure plate and removing the tree shrew brain slice.

[0016] Compared with the prior art, the present invention provides a mold and slicing method for making TTC-stained brain slices of tree shrews. By setting up a blade, a placement groove and a blade groove, when making tree shrew brain slices, the tree shrew brain block can be placed in the placement groove, and then the pressure plate is pressed to drive the blade to insert into the blade groove. The blade separates the tree shrew brain block into slices, and the sliced ​​tree shrew brain block falls stably into the placement groove. The whole device is simple to operate and does not affect the shape of the tree shrew brain slices, making it convenient for operators to handle the tree shrew brain slices. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0018] Figure 1 A schematic diagram of the overall structure provided for an embodiment of the present invention. Figure 1 ;

[0019] Figure 2 A schematic diagram of the overall structure provided for an embodiment of the present invention. Figure 2 ;

[0020] Figure 3 This is a partial cross-sectional structural schematic diagram provided for an embodiment of the present invention;

[0021] Figure 4 A flowchart illustrating the method provided in an embodiment of the present invention.

[0022] Explanation of reference numerals in the attached figures:

[0023] 1. Mounting housing; 2. Telescopic rod; 3. Spring; 4. Pressure plate; 5. Blade; 6. Placement slot; 7. Blade groove. Detailed Implementation

[0024] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0025] As attached Figure 1 To be continued Figure 4 As shown:

[0026] Example:

[0027] This invention provides a mold for preparing TTC-stained brain slices from tree shrews, characterized in that it comprises:

[0028] Mounting shell 1, the top of mounting shell 1 is provided with multiple knife grooves 7 to form a cutting area, the upper surface of mounting shell 1 is provided with a placement groove 6 in the cutting area that communicates with the knife grooves 7, mounting shell 1 is provided with a telescopic hole, the telescopic rod 2 is inserted into the telescopic hole, and a spring 3 is provided in the telescopic hole;

[0029] The telescopic rod 2 is slidably connected to the mounting shell 1, and a pressure plate 4 is hinged to one side of the telescopic rod 2. A blade 5 is connected to the pressure plate 4, and the blade 5 is arranged opposite to the blade groove 7.

[0030] As can be seen from the above, by setting the blade 5, the placement groove 6 and the knife groove 7, when making tree shrew brain slices, the tree shrew brain block can be placed in the placement groove 5, and then the pressure plate 4 is pressed to drive the blade 5 to insert into the knife groove 7. The blade 5 separates the tree shrew brain block into slices, and the sliced ​​tree shrew brain block falls stably into the placement groove 6. The whole device is simple to operate and will not affect the shape of the tree shrew brain slices, making it convenient for operators to pick up the tree shrew brain slices.

[0031] The placement slot 6 is elliptical in shape to conform to the shape of the tree shrew brain block and can stably hold the tree shrew brain block.

[0032] The blade 5 is slidably engaged with the pressure plate 4, allowing the user to slide off the damaged blade 5 and replace it with a new one.

[0033] A method for preparing TTC-stained brain slices from tree shrews, comprising the following steps:

[0034] Place the tree shrew brain block in placement slot 6;

[0035] Rotate the pressure plate 4 so that the blade 5 abuts against the tree shrew brain block, and press the pressure plate 4 to drive the telescopic rod 2 to compress the spring 3. Multiple pressure plates 4 are interleaved in the blade groove 7 to slice the tree shrew brain block.

[0036] Release the pressure plate 4, the compressed spring 3 returns to its original position and pushes the telescopic rod 2 upward, lifting the pressure plate 4 and taking out the tree shrew brain block slice.

[0037] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A mold for preparing TTC-stained brain slices from tree shrews, characterized in that, include: Mounting shell (1), the top of the mounting shell (1) is provided with multiple knife grooves (7) to form a cutting area, and the upper surface of the mounting shell (1) is provided with a placement groove (6) that communicates with the knife grooves (7). The telescopic rod (2) is slidably connected to the mounting shell (1), and a pressure plate (4) is hinged on one side of the telescopic rod (2). A blade (5) is connected to the pressure plate (4), and the blade (5) is arranged opposite to the blade groove (7).

2. A mold for preparing TTC-stained brain slices of tree shrews according to claim 1, characterized in that, The mounting shell (1) has a telescopic hole, the telescopic rod (2) is inserted into the telescopic hole, and a spring (3) is provided in the telescopic hole.

3. A mold for preparing TTC-stained brain slices from tree shrews according to claim 1, characterized in that, The placement slot (6) is elliptical in shape.

4. A mold for preparing TTC-stained brain slices of tree shrews according to claim 1, characterized in that, The blade (5) is slidably engaged with the pressure plate (4).

5. A method for preparing TTC-stained brain slices from tree shrews, characterized in that the steps include... include: Place the tree shrew brain block in the placement slot (6); Rotate the pressure plate (4) so ​​that the blade (5) abuts against the tree shrew brain block and press the pressure plate (4) to drive the telescopic rod (2) to compress the spring (3). Multiple pressure plates (4) are interlocked in the blade groove (7) to slice the tree shrew brain block. Release the pressure plate (4), the compressed spring (3) returns to its original position and pushes the telescopic rod (2) upward, lift the pressure plate (4), and take out the tree shrew brain block slice.

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