Process for the preparation of a fermentation mixture based on the aqueous biotransformation of aescin and equipment therefor

Abstract

The present application belongs to the technical field of fermentation mixing, and discloses a preparation method and equipment of a fermentation mixed product based on the bioconversion of aesculus hippocastanum water solubility. The preparation equipment of the fermentation mixed product based on the bioconversion of aesculus hippocastanum water solubility comprises a shell, a feeding port arranged at the top of the outer side of the shell, a discharging port arranged at the bottom of the outer side of the shell, a partition plate arranged at the center of the inner side of the shell, a rotating rod fixed at the center of the partition plate, and the partition plate fixedly installed in the middle of the rotating rod. The rotating rod is used to make the stirring frame rotate synchronously with the rotating rod by means of the clamping rod and the square and round groove. When the fermentation mixture at the bottom of the partition plate is stirred by the rotating stirring frame, the support is driven to move up and down in the inclined groove by the top rod, so that the rotating plate rotates out of and into the side groove, the mixing degree of the fermentation mixture in the shell is improved, and the stirring and mixing effect of the fermentation mixture in the shell is improved.

Description

Technical Field

[0001] This invention belongs to the field of fermentation mixing technology, and specifically relates to a method and equipment for preparing fermentation mixture products based on the biotransformation of water-soluble horse chestnut. Background Technology

[0002] With the increasing attention paid to natural, green, healthy, and safe plant materials in cosmetics, the development and utilization of plant-based active substances and the research and development of pure natural cosmetics have become one of the most active themes in the cosmetics industry. Horse chestnut extract, a natural active ingredient extracted from the horse chestnut tree, contains 65 active substances including various saponins and flavonoids. It possesses powerful antioxidant properties, scavenging free radicals, reducing inflammation in fragile skin, anti-aging and protecting cells, antibacterial properties, regulating scalp microecology, blocking UV damage, and promoting microcirculation and maintaining normal cell metabolism by inhibiting hyaluronidase. It has significant potential for development and application in various products such as shampoos, shower gels, face creams, lotions, and toothpaste. Like the bark of the horse chestnut tree, the leaf tissue contains coumarin glycosides such as scopolamine, fraxin, and aescin. A series of quercetin flavonoid glycosides (such as quercetin, rutin, isoquercitrin, and quercetin 3-arabinoside) and corresponding glucosides have also been detected in the leaf tissue. In addition to these glycosides, esculin was also detected (but only in trace amounts), as well as resveratrol, cis- and trans-polypentenols, amino acids, fatty acids, and sterols (sitosterol, stigmasterol, and brassosterol).

[0003] The main active ingredient in European horse chestnut is aescin, also known as aescinol, a triterpenoid saponin compound. Aescinol is a potent hemolytic saponin and is the main component responsible for its anti-edema, anti-exudation, and vascular protective effects. It can strengthen veins and capillaries, and inhibit an enzyme called hyaluronidase, which can break down proteoglycans (structural components of capillary walls). Horse chestnut extract also contains abundant flavonoids, such as derivatives of quercetin and kaempferol, which can all protect blood vessels.

[0004] 1. Promotes microcirculation

[0005] It improves blood and water circulation, soothes the skin, and accelerates skin cell metabolism.

[0006] 2. Inhibit enzyme activity

[0007] It inhibits enzymes closely related to the integrity of skin texture, such as collagenase and hyaluronidase. These enzymes can catalyze the destruction of the integrity of venous structures and increase capillary exudation. However, the active ingredients of horse chestnut can effectively inhibit these enzymes.

[0008] 3. Anti-inflammatory

[0009] It can directly act on histamine, which causes inflammation, thereby fighting inflammation.

[0010] 4. Protect capillaries

[0011] It can promote wound healing, improve blood vessel walls, fill tiny gaps in capillaries, and promote the self-repair and strengthening of skin cells.

[0012] Currently, when preparing fermentation mixtures from horse chestnut fruit, the mixture of horse chestnut aqueous solution is usually stirred by continuously rotating a stirring paddle. Because the stirring direction is unidirectional, this stirring equipment generally has low stirring efficiency and uneven stirring.

[0013] Therefore, it is necessary to invent a method and equipment for preparing fermentation mixtures based on the biotransformation of water-soluble horse chestnut to solve the above problems. Summary of the Invention

[0014] To address the aforementioned problems, this invention provides a method and apparatus for preparing fermentation mixtures based on the water-soluble biotransformation of horse chestnut, thereby resolving the issues raised in the background section.

[0015] To achieve the above objectives, the present invention provides the following technical solution: a fermentation mixture preparation device based on the water-soluble biotransformation of horse chestnut, comprising a shell, an inlet provided at the top of the outer circumference of the shell, an outlet provided at the bottom of the outer circumference of the shell, a partition provided at the center of the interior of the shell, a rotating rod fixed at the center of the partition, the partition being fixedly installed in the middle of the rotating rod, a motor output terminal connected to the top of the rotating rod, multiple communicating structures installed on the surface of the partition, through grooves provided on the surface of the partition that cooperate with the multiple communicating structures, a stirring rack provided at the bottom of the partition, a square-round groove provided at the center of the top surface of the stirring rack, and a locking rod fixed at the center of the bottom end of the rotating rod that corresponds to the square-round groove, an inner rod fixed at the center of the bottom of the inner side of the shell, the top of the inner rod being movably inserted into the center of the stirring rack, the stirring rack being rotatably sleeved on the surface of the inner rod, a first spring sleeved on the surface of the inner rod, the top of the first spring being in contact with the bottom surface of the stirring rack, the bottom of the first spring being in contact with the bottom surface of the inner side of the shell, and a cover plate covering the opening at the top of the shell.

[0016] Furthermore, the cover plate has side plates fixed to its circumferential side, and the outer side of the housing has two ear plates fixed to its bottom. The two ear plates correspond one-to-one with the two side plates. The bottom of the side plate is fixed with a screw that passes through the ear plate, and the bottom end of the screw is screwed with a nut.

[0017] Furthermore, a pressure plate is provided at the top opening of the housing, and two pressure rods that penetrate the cover plate are fixedly fixed on the top surface of the pressure plate. A screen plate is provided between the pressure plate and the partition plate. The top of the rotating rod passes through the center of the screen plate, the pressure plate and the cover plate in sequence. The screen plate has multiple holes evenly arranged, and multiple support rods are fixed in a ring on the bottom surface of the screen plate. The bottom end of the support rod is attached to the top surface of the partition plate. The inner end of the feed inlet is located between the pressure plate and the screen plate.

[0018] Furthermore, the connecting structure includes a vertically arranged connecting pipe, the top end of which corresponds to a through groove penetrating the surface of the partition. A circular plate is fixed to the top end of the connecting pipe, and the diameter of the circular plate is larger than the diameter of the through groove. When the partition rotates so that the circular plate contacts the bottom end of the support rod, the bottom end of the support rod is positioned between the two outermost circular plates. The distance between the two outermost circular plates is smaller than the diameter of the support rod. Both the top and bottom of the outer circumference of the connecting pipe are provided with through openings, and the distance between the top through opening and the bottom through opening is greater than the thickness of the partition.

[0019] Furthermore, the bottom end of the connecting pipe has two side rods fixedly fixed to each other, and vertical rods are provided on both sides of the connecting pipe. The top ends of the vertical rods are fixedly connected to the bottom surface of the partition. The bottom ends of the two vertical rods respectively pass through the ends of the two side rods. An elastic element is sleeved on the surface of the vertical rod. The top end of the elastic element is connected to the bottom surface of the partition, and the bottom end of the elastic element is connected to the surface of the side rod.

[0020] Furthermore, the stirring rack includes two side frames, with multiple side grooves arranged opposite each other on both sides of the side frames. A rotating plate is rotatably engaged inside the side groove. A protruding rod is provided on the top of each side of the rotating plate. The rotating plate rotates with the side groove of the side frame using the protruding rod. A top rod is provided between the two rotating plates, penetrating the top of the side frame. A bottom rod is provided at the bottom end of the top rod, penetrating the bottom of the side frame. The top end of the bottom rod is spirally engaged with the bottom end of the top rod. The top end of the top rod corresponds to the bottom end of the connecting pipe.

[0021] Furthermore, a second spring is sleeved on the top end of the top rod, the bottom end of the second spring is connected to the top surface of the side frame, the top end of the second spring is connected to the top end of the top rod, and two supports are fixed at the bottom end of the top rod. The inner side of the rotating plate is provided with inclined grooves corresponding to the supports, and the supports are slidably engaged in the inclined grooves.

[0022] Furthermore, the two rotating plates on both sides of the top rod are arranged opposite each other, and the distance between the top ends of the two opposite inclined grooves is less than the distance between their bottom ends. When the bracket moves upward inside the inclined groove, the bracket pushes the rotating plate to rotate out of the side groove of the side frame.

[0023] This invention provides a method for preparing a fermentation mixture based on the water-soluble biotransformation of horse chestnut. The method utilizes the aforementioned equipment for preparing the fermentation mixture based on the water-soluble biotransformation of horse chestnut and includes the following steps:

[0024] S1. Obtain horse chestnuts and crush them to obtain horse chestnut seed powder;

[0025] S2. Activate the lactic acid bacteria and yeast separately to obtain secondary seed culture of lactic acid bacteria and secondary seed culture of yeast, respectively.

[0026] S3. The secondary seed liquid of lactic acid bacteria and secondary seed liquid of yeast obtained in S2 are mixed with the horse chestnut seed powder in S1 to obtain a mixture. The mixture is introduced into the shell through the feed port and undergoes coarse fermentation at the top of the partition. The motor is started and the motor drives the partition to rotate through the rotating rod. The partition drives the support rod to rotate through the circular plate at the top of the connecting structure. The support rod drives the sieve plate to rotate, which facilitates the initial mixing of the mixture. During the initial mixing process, urea is added into the shell through the feed port to obtain the fermented mixture.

[0027] S4. Press the top of the pressure rod, and the pressure rod will drive the pressure plate to press down. After the bottom surface of the pressure plate is in contact with the top surface of the sieve plate, the pressure plate will use the support rod at the bottom of the sieve plate to drive the partition to move down. The rotating rod at the bottom of the partition will drive the stirring frame to squeeze the first spring on the surface of the inner rod until the bottom end of the bottom rod contacts the bottom surface of the inner side of the shell. The reaction force of the bottom surface of the inner side of the shell on the bottom rod will cause the bottom rod to drive the top rod to move up inside the side frame. The moving top rod will cause the connecting pipe to move up inside the through groove of the partition until the top opening moves to the top of the partition. The fermentation mixture at the top of the partition will flow to the bottom of the partition through the opening and the connecting pipe.

[0028] S5. When the pressure rod is pressed, the pressure rod uses the screen plate and support rod to move the partition plate downwards. The partition plate, through the connecting pipe, squeezes and moves the top rod downwards. During the downward movement of the top rod, the support moves downwards inside the inclined groove. The downward movement of the support causes the rotating plate to rotate into the side groove. When the bottom end of the bottom rod contacts the bottom surface of the inner side of the housing, the reaction force of the bottom surface of the inner side of the housing on the bottom rod causes the bottom rod to drive the top rod upwards. The upward movement of the top rod pulls the second spring at the top of the side frame. The upward movement of the top rod causes the support to move upwards inside the inclined groove. The upward movement of the support causes... The rotating plate rotates out from inside the side groove; the pressure applied to the top of the pressure rod is released, the first spring rebounds and causes the stirring rack to move upward. At this time, the bottom end of the bottom rod separates from the bottom surface of the inner side of the shell, and the elastic force of the second spring causes the top rod to move downward. The top rod drives the support to move downward inside the inclined groove. The downward-moving support causes the rotating plate to rotate into the side groove. After the rotating plate is completely inside the side groove, the bottom end of the rotating rod drives the stirring rack to stir the fermentation mixture. The rotating plate increases the degree of mixing of the fermentation mixture inside the shell, and the final product is obtained.

[0029] S6. Discharge the final product through the discharge port.

[0030] The technical effects and advantages of this invention are as follows:

[0031] 1. This invention uses a rotating rod, a clamp, and a square and round groove to make the stirring rack rotate synchronously with the rotating rod. When the rotating stirring rack stirs the fermentation mixture at the bottom of the partition, the top rod drives the support to move up and down inside the inclined groove, thereby making the rotating plate rotate in and out of the side groove, improving the mixing degree of the fermentation mixture inside the shell and improving the stirring and mixing effect of the fermentation mixture inside the shell.

[0032] 2. This invention achieves real-time isolation and flow guidance of the fermentation mixture by causing the side rod at the bottom of the connecting pipe to move upward inside the channel as the connecting pipe moves upward inside the channel. The upward-moving side rod cooperates with the elastic element on the surface of the vertical rod to compress the elastic element until the opening at the top of the connecting pipe moves to the top of the partition. After the connecting structure completes the flow guidance of the fermentation mixture, the pressure on the connecting pipe is released, and the elastic force of the elastic element pulls the connecting pipe downward inside the channel until the opening at the top of the connecting pipe moves to the bottom of the partition. The connecting structure and the partition work together to achieve the real-time isolation and flow guidance of the fermentation mixture.

[0033] 3. The present invention uses a pressure plate and a sieve plate to squeeze the solid mixture at the top of the sieve plate, which can squeeze out the fermentation mixture remaining on the surface of the solid mixture and avoid fermentation mixture residue. Attached Figure Description

[0034] Figure 1 This is an overall schematic diagram of the fermentation mixture preparation equipment based on the water-soluble biotransformation of horse chestnut according to an embodiment of the present invention;

[0035] Figure 2 This is a schematic diagram of the internal components of the housing according to an embodiment of the present invention;

[0036] Figure 3 This is a schematic diagram of the bottom component of the partition in an embodiment of the present invention;

[0037] Figure 4 This is a three-dimensional cross-sectional schematic diagram of the connected structure according to an embodiment of the present invention;

[0038] Figure 5 This is a three-dimensional cross-sectional schematic diagram of the side frame according to an embodiment of the present invention;

[0039] Figure 6 This is a schematic diagram of the two rotating plates inside the side frame of this invention relative to each other;

[0040] Figure 7 This is a schematic diagram of the inner side of the rotating plate according to an embodiment of the present invention;

[0041] In the diagram: 1. Shell; 2. Inlet; 3. Outlet; 4. Partition; 5. Rotating rod; 6. Mixing rack; 7. Square and round groove; 8. Clamping rod; 9. Inner rod; 10. First spring; 11. Cover plate; 12. Ear plate; 13. Nut; 14. Pressure plate; 15. Pressure rod; 16. Screen plate; 17. Support rod; 18. Connecting pipe; 19. Through port; 20. Side rod; 21. Vertical rod; 22. Elastic element; 23. Side frame; 24. Rotating plate; 25. Top rod; 26. Bottom rod; 27. Second spring; 28. Support; 29. ​​Inclined groove. Detailed Implementation

[0042] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments.

[0043] This invention provides equipment for preparing fermentation mixtures based on the water-soluble biotransformation of horse chestnut, such as... Figures 1 to 5 As shown, the device includes a housing 1. A feed inlet 2 is located at the top of the outer circumference of the housing 1, and a discharge outlet 3 is located at the bottom of the outer circumference of the housing 1. A partition 4 is located at the center of the housing 1. A rotating rod 5 is fixed at the center of the partition 4, and the partition 4 is fixedly installed in the middle of the rotating rod 5. A motor output is connected to the top of the rotating rod 5. Multiple communicating structures are installed on the surface of the partition 4, and through grooves that mate with these communicating structures are provided on the surface of the partition 4. A stirring rack 6 is located at the bottom of the partition 4. A square-round groove 7 is provided at the center of the top surface, and a locking rod 8 corresponding to the square-round groove 7 is fixed at the center of the bottom end of the rotating rod 5. An inner rod 9 is fixed at the center of the bottom of the inner side of the housing 1. The top end of the inner rod 9 is movably inserted into the center of the stirring rack 6. The stirring rack 6 is rotatably sleeved on the surface of the inner rod 9. A first spring 10 is sleeved on the surface of the inner rod 9. The top end of the first spring 10 is in contact with the bottom surface of the stirring rack 6, and the bottom end of the first spring 10 is in contact with the bottom surface of the inner side of the housing 1. A cover plate 11 is placed at the top opening of the housing 1. When the partition 4 is placed inside the housing 1, the locking rod 8 at the bottom of the rotating rod 5 corresponds to the square and round groove 7 at the top of the stirring rack 6 until the locking rod 8 is locked inside the square and round groove 7. When the cover plate 11 is placed on the top opening of the housing 1, the top of the rotating rod 5 passes through the center of the top surface of the cover plate 11. The top of the rotating rod 5 is connected to the motor output terminal. The motor works to drive the partition 4 to rotate inside the housing 1 using the rotating rod 5. The bottom of the rotating rod 5 drives the stirring rack 6 to rotate synchronously using the locking rod 8.

[0044] After the mixture is fed into the shell 1 through the inlet 2, the outlet 3 is sealed. The partition 4 rotates to facilitate the initial stirring of the mixture. The mixture at the top of the partition 4 enters the bottom of the partition 4 through the connecting structure. The rotating stirring rack 6 further stirs and mixes the mixture inside the shell 1. After stirring and mixing, fermentation is carried out until the final product is formed. The outlet 3 is opened and the final product is discharged through the outlet 3, thus completing the preparation of the final product.

[0045] exist Figure 1 and Figure 2 In this structure, side plates are fixed to the circumferential sides of the cover plate 11, and two ear plates 12 are fixed to the bottom of the outer circumferential side of the housing 1. The two ear plates 12 correspond one-to-one with the two side plates. A screw rod is fixed to the bottom of the side plate, penetrating the ear plate 12, and a nut 13 is screwed onto the bottom end of the screw rod. When the cover plate 11 is placed over the top opening of the housing 1, the two side plates of the cover plate 11 correspond to the two ear plates 12 at the top of the housing 1, and the bottom surfaces of the side plates are correspondingly attached to the top surfaces of the ear plates 12. At this time, the screw rod at the bottom of the side plate penetrates the center of the ear plate 12, and the nut 13 is screwed onto the bottom end of the screw rod, making it convenient for the cover plate 11 to seal the top opening of the housing 1.

[0046] exist Figure 2 In this assembly, a pressure plate 14 is provided at the top opening of the housing 1. Two pressure rods 15, penetrating the cover plate 11, are fixedly fixed on the top surface of the pressure plate 14. A sieve plate 16 is provided between the pressure plate 14 and the partition plate 4. The top of the rotating rod 5 passes through the center of the sieve plate 16, the pressure plate 14, and the cover plate 11 in sequence. The sieve plate 16 has multiple evenly arranged perforations, and multiple support rods 17 are fixed in a ring on the bottom surface of the sieve plate 16. The bottom end of the support rods 17 is attached to the top surface of the partition plate 4. The inner end of the feed inlet 2 is located between the pressure plate 14 and the sieve plate 16. After the partition plate 4 is installed inside the housing 1, the sieve plate 16 and the pressure plate 14 are placed inside the housing 1 in sequence. Finally, the cover plate 11 is placed on the top opening of the housing 1, at which point the top of the rotating rod 5 passes through the center of the sieve plate 16, the pressure plate 14, and the cover plate 11 in sequence. This completes the installation of the entire preparation equipment.

[0047] Pull the pressure rod 15 upward, and the pressure rod 15 pulls the pressure plate 14 upward inside the shell 1 until the top surface of the pressure plate 14 is in contact with the bottom surface of the cover plate 11. Then, the mixture is introduced into the shell 1 through the feed port 2. The mixture falls onto the top surface of the sieve plate 16 and is filtered by the sieve plate 16. The perforations on the surface of the sieve plate 16 facilitate the separation of liquid and solid in the mixture. When the liquid level of the mixture is level with the top surface of the sieve plate 16, stop introducing the mixture, release the pressure rod 15, and the pressure plate 14 moves downward until the bottom surface of the pressure plate 14 is in contact with the top of the sieve plate 16. This prevents external impurities from mixing into the mixture inside the shell 1 through the feed port 2. When the motor works, the rotating rod 5 drives the partition plate 4 to rotate. The partition plate 4 uses the circular plate at the top of the connecting structure to drive the support rod 17 to stir the mixture, which facilitates the initial stirring and fermentation treatment of the mixture.

[0048] exist Figures 2 to 4 In this structure, the connecting structure includes a vertically arranged connecting pipe 18. The top end of the connecting pipe 18 corresponds to a through groove penetrating the surface of the partition 4. A circular plate is fixed to the top end of the connecting pipe 18, and the diameter of the circular plate is larger than the diameter of the through groove. When the partition 4 rotates so that the circular plate contacts the bottom end of the support rod 17, the bottom end of the support rod 17 is positioned between the two outermost circular plates. The distance between the two outermost circular plates is smaller than the diameter of the support rod 17. The top and bottom of the outer circumference of the connecting pipe 18 are provided with openings 19, and the distance between the top opening 19 and the bottom opening 19 is greater than the thickness of the partition 4. When the rotating rod 5 drives the partition 4 to rotate, the partition 4 uses the connecting pipe 18 to drive the circular plate to rotate. When the circular plate at the top end of the connecting pipe 18 contacts the bottom end of the support rod 17, the circular plate uses the support rod 17 and the sieve plate 16 to stir the mixture at the top of the partition 4, which can perform preliminary stirring of the mixture to obtain a fermentation mixture.

[0049] When the connecting pipe 18 moves upward inside the channel, the opening 19 on the top of the surface of the connecting pipe 18 moves to the top of the partition 4. The fermentation mixture at the top of the partition 4 enters the bottom of the partition 4 through the opening 19 and the connecting pipe 18, and the flow of the fermentation mixture is realized by the interconnection structure.

[0050] The bottom end of the connecting pipe 18 has two side rods 20 fixedly fixed to each other. Vertical rods 21 are provided on both sides of the connecting pipe 18, and the top of the vertical rods 21 are fixedly connected to the bottom surface of the partition 4. The bottom ends of the two vertical rods 21 respectively pass through the ends of the two side rods 20. An elastic element 22 is sleeved on the surface of the vertical rod 21. The top end of the elastic element 22 is connected to the bottom surface of the partition 4, and the bottom end of the elastic element 22 is connected to the surface of the side rods 20.

[0051] When the connecting pipe 18 moves upward inside the channel, the side rod 20 at the bottom of the connecting pipe 18 moves upward on the surface of the vertical rod 21, and the upward-moving side rod 20 cooperates to squeeze the elastic element 22 on the surface of the vertical rod 21 until the opening 19 at the top of the surface of the connecting pipe 18 moves to the top of the partition 4. After the connecting structure completes the flow of the fermentation mixture, the pressure on the connecting pipe 18 is released, and the elastic force of the elastic element 22 pulls the connecting pipe 18 downward inside the channel until the opening 19 at the top of the surface of the connecting pipe 18 moves to the bottom of the partition 4. The connecting structure and the partition 4 work together to achieve the real-time isolation and flow guidance effect of the fermentation mixture.

[0052] During the process of guiding the fermentation mixture from the top of the partition 4 to the bottom of the partition 4, the pressure plate 14 and the sieve plate 16 work together to squeeze the solid mixture on the top of the sieve plate 16, which can squeeze out the fermentation mixture remaining on the surface of the solid mixture and avoid fermentation mixture residue.

[0053] exist Figure 2 , Figure 5 and Figure 6 In the process, the stirring rack 6 includes two side frames 23. Multiple side grooves are arranged opposite each other on both sides of the side frames 23. A rotating plate 24 is rotatably engaged inside the side groove. A protruding rod is provided on the top of both sides of the rotating plate 24. The rotating plate 24 is rotatably engaged with the side groove of the side frame 23 by means of the protruding rod. A top rod 25 is provided between the two rotating plates 24, penetrating the top of the side frame 23. A bottom rod 26 is provided at the bottom end of the top rod 25, penetrating the bottom of the side frame 23. The top end of the bottom rod 26 is spirally engaged with the bottom end of the top rod 25. The top end of the top rod 25 corresponds to the bottom end of the connecting pipe 18. After the partition 4 is placed inside the shell 1, the bottom end of the rotating rod 5 is inserted into the square and round groove 7 at the top of the stirring rack 6 using the clamping rod 8. At this time, the bottom ends of the connecting pipes 18 of the multiple connecting structures correspond one-to-one with the top ends of the multiple top rods 25, and the bottom ends of the connecting pipes 18 are in contact with the top ends of the top rods 25. When the rotating rod 5 rotates, the rotating rod 5 uses the clamping rod 8 and the square and round groove 7 to make the stirring rack 6 rotate synchronously with the rotating rod 5, and the fermentation mixture at the bottom of the partition 4 is stirred by rotating the stirring rack 6.

[0054] Pressing the top of the pressure rod 15 causes the pressure plate 14 to press down. After the bottom surface of the pressure plate 14 is in contact with the top surface of the sieve plate 16, the pressure plate 14 uses the support rod 17 at the bottom of the sieve plate 16 to move the partition 4 down. The rotating rod 5 at the bottom of the partition 4 drives the stirring frame 6 to squeeze the first spring 10 on the surface of the inner rod 9 until the bottom end of the bottom rod 26 contacts the bottom surface of the inner side of the shell 1. The reaction force of the bottom surface of the inner side of the shell 1 on the bottom rod 26 causes the bottom rod 26 to drive the top rod 25 to move up inside the side frame 23. The moving top rod 25 causes the connecting pipe 18 to move up inside the through groove of the partition 4 until the top opening 19 moves to the top of the partition 4. The fermentation mixture at the top of the partition 4 flows to the bottom of the partition 4 through the opening 19 and the connecting pipe 18.

[0055] exist Figures 5 to 7In this configuration, a second spring 27 is sleeved at the top of the top rod 25. The bottom end of the second spring 27 is connected to the top surface of the side frame 23, and the top end of the second spring 27 is connected to the top of the top rod 25. Two supports 28 are fixed at the bottom of the top rod 25. The inner side of the rotating plate 24 is provided with inclined grooves 29 corresponding to the supports 28, and the supports 28 are slidably engaged inside the inclined grooves 29. The two rotating plates 24 on both sides of the top rod 25 are arranged opposite each other, and the distance between the top ends of the two opposite inclined grooves 29 is less than the distance between their bottom ends. When the supports 28 move upward inside the inclined grooves 29, the supports 28 push the rotating plates 24 to rotate out of the side grooves of the side frame 23.

[0056] When the pressure rod 15 is pressed, the pressure rod 15 uses the screen plate 16 and the support rod 17 to make the partition plate 4 move down. The partition plate 4 uses the connecting pipe 18 to squeeze and make the top rod 25 move down. During the downward movement of the top rod 25, the support 28 moves down inside the inclined groove 29. The downward movement of the support 28 makes the rotating plate 24 rotate into the side groove.

[0057] When the bottom end of the bottom rod 26 contacts the inner bottom surface of the housing 1, the reaction force of the inner bottom surface of the housing 1 on the bottom rod 26 causes the bottom rod 26 to drive the top rod 25 to move upward. The upward-moving top rod 25 pulls the second spring 27 at the top of the side frame 23. The upward-moving top rod 25 causes the bracket 28 to move upward inside the inclined groove 29. The upward-moving bracket 28 causes the rotating plate 24 to rotate out from inside the side groove.

[0058] When the pressure applied to the top of the pressure rod 15 is released, the first spring 10 rebounds, causing the stirring rack 6 to move upward. At this time, the bottom end of the bottom rod 26 separates from the inner bottom surface of the shell 1. The elastic force of the second spring 27 causes the top rod 25 to move downward. The top rod 25 drives the support 28 to move downward inside the inclined groove 29. The downward moving support 28 causes the rotating plate 24 to rotate into the side groove. After the rotating plate 24 is completely inside the side groove, the bottom end of the rotating rod 5 drives the stirring rack 6 to stir the fermentation mixture. The rotating plate 24 increases the degree of mixing of the fermentation mixture inside the shell 1, thus obtaining the final product.

[0059] This invention provides a method for preparing a fermentation mixture based on the water-soluble biotransformation of horse chestnut. The method utilizes the aforementioned equipment for preparing the fermentation mixture based on the water-soluble biotransformation of horse chestnut, as described above. Figures 1 to 7 As shown, it includes the following steps:

[0060] S1. Obtain horse chestnuts and crush them to obtain horse chestnut seed powder;

[0061] S2. Activate the lactic acid bacteria and yeast separately to obtain secondary seed culture of lactic acid bacteria and secondary seed culture of yeast, respectively.

[0062] S3. The secondary seed liquid of lactic acid bacteria and secondary seed liquid of yeast obtained in S2 are mixed with the horse chestnut seed powder in S1 to obtain a mixture. The mixture is introduced into the shell 1 through the feed inlet 2 and undergoes coarse fermentation at the top of the partition 4. The motor is started and the motor drives the partition 4 to rotate through the rotating rod 5. The partition 4 drives the support rod 17 to rotate through the circular plate at the top of the connecting structure. The support rod 17 drives the sieve plate 16 to rotate, which facilitates the initial mixing of the mixture. During the initial mixing process, urea is added into the shell 1 through the feed inlet 2 to obtain the fermented mixture.

[0063] S4. Press the top of the pressure rod 15. The pressure rod 15 drives the pressure plate 14 to press down. After the bottom surface of the pressure plate 14 is in contact with the top surface of the sieve plate 16, the pressure plate 14 uses the support rod 17 at the bottom of the sieve plate 16 to drive the partition plate 4 to move down. The rotating rod 5 at the bottom of the partition plate 4 drives the stirring frame 6 to squeeze the first spring 10 on the surface of the inner rod 9 until the bottom end of the bottom rod 26 contacts the bottom surface of the inner side of the shell 1. The reaction force of the bottom surface of the inner side of the shell 1 on the bottom rod 26 causes the bottom rod 26 to drive the top rod 25 to move up inside the side frame 23. The moving top rod 25 causes the connecting pipe 18 to move up inside the through groove of the partition plate 4 until the top opening 19 moves to the top of the partition plate 4. The fermentation mixture at the top of the partition plate 4 flows to the bottom of the partition plate 4 through the opening 19 and the connecting pipe 18.

[0064] S5. When pressing the pressure rod 15, the pressure rod 15 uses the sieve plate 16 and the support rod 17 to make the partition plate 4 move downward. The partition plate 4 uses the connecting pipe 18 to squeeze and make the top rod 25 move downward. During the downward movement of the top rod 25, the support 28 moves downward inside the inclined groove 29. The downward movement of the support 28 makes the rotating plate 24 rotate into the side groove. When the bottom end of the bottom rod 26 contacts the inner bottom surface of the housing 1, the reaction force of the inner bottom surface of the housing 1 on the bottom rod 26 makes the bottom rod 26 drive the top rod 25 to move upward. The upward movement of the top rod 25 pulls the second spring 27 at the top of the side frame 23. The upward movement of the top rod 25 makes the support 28 move upward inside the inclined groove 29. The moving support 28 causes the rotating plate 24 to rotate out of the side groove; the pressure applied to the top of the pressure rod 15 is released, the first spring 10 rebounds and causes the stirring rack 6 to move upward. At this time, the bottom end of the bottom rod 26 separates from the inner bottom surface of the shell 1, and the elastic force of the second spring 27 causes the top rod 25 to move downward. The top rod 25 drives the support 28 to move downward inside the inclined groove 29. The moving support 28 causes the rotating plate 24 to rotate into the side groove. After the rotating plate 24 is completely inside the side groove, the bottom end of the rotating rod 5 drives the stirring rack 6 to stir the fermentation mixture. The rotating plate 24 increases the degree of mixing of the fermentation mixture inside the shell 1, and the final product is obtained.

[0065] S6. The final product is discharged through the discharge port 3.

[0066] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it.

Claims

1. A fermentation mixture preparation device based on the water-soluble biotransformation of horse chestnut, comprising a shell (1), characterized in that: The shell (1) has a feed inlet (2) at the top of its outer circumference and a discharge outlet (3) at the bottom of its outer circumference. A partition (4) is located at the center of the shell (1). A rotating rod (5) is fixed at the center of the partition (4), and the partition (4) is fixedly installed in the middle of the rotating rod (5). The top of the rotating rod (5) is connected to the output end of a motor. Multiple connecting structures are installed on the surface of the partition (4), and through grooves that cooperate with these connecting structures are provided on the surface of the partition (4). A stirring rack (6) is located at the bottom of the partition (4), and a stirring rack (6) is located at the center of its top surface. There is a square and round groove (7), and a locking rod (8) corresponding to the square and round groove (7) is fixed at the center of the bottom end of the rotating rod (5). An inner rod (9) is fixed at the center of the bottom inside the shell (1). The top end of the inner rod (9) is movably inserted into the center of the stirring rack (6). The stirring rack (6) is rotated and sleeved on the surface of the inner rod (9). A first spring (10) is sleeved on the surface of the inner rod (9). The top end of the first spring (10) is in contact with the bottom surface of the stirring rack (6). The bottom end of the first spring (10) is in contact with the bottom surface of the inner side of the shell (1). A cover plate (11) is placed at the top opening of the shell (1).

2. The fermentation mixture preparation equipment based on the water-soluble biotransformation of horse chestnut as described in claim 1, characterized in that: The cover plate (11) has side plates fixed to each other on its circumferential side. The outer side of the shell (1) has two ear plates (12) fixed to each other at its bottom. The two ear plates (12) correspond to the two side plates one by one. The bottom of the side plate is fixed with a screw that passes through the ear plate (12). The bottom end of the screw is screwed with a nut (13).

3. The fermentation mixture preparation equipment based on the water-soluble biotransformation of horse chestnut as described in claim 1, characterized in that: A pressure plate (14) is provided at the top opening of the housing (1). Two pressure rods (15) that penetrate the cover plate (11) are fixed on the top surface of the pressure plate (14). A screen plate (16) is provided between the pressure plate (14) and the partition plate (4). The top of the rotating rod (5) passes through the center of the screen plate (16), the pressure plate (14) and the cover plate (11) in sequence. The screen plate (16) has multiple holes evenly arranged. Multiple support rods (17) are fixed in a ring on the bottom surface of the screen plate (16). The bottom end of the support rod (17) is attached to the top surface of the partition plate (4). The inner end of the feed inlet (2) is located between the pressure plate (14) and the screen plate (16).

4. The fermentation mixture preparation equipment based on the biotransformation of water-soluble horse chestnut according to claim 3, characterized in that: The connecting structure includes a vertically arranged connecting pipe (18). The top end of the connecting pipe (18) corresponds to a through groove penetrating the surface of the partition (4). A circular plate is fixed at the top end of the connecting pipe (18), and the diameter of the circular plate is larger than the diameter of the through groove. When the partition (4) rotates so that the circular plate contacts the bottom end of the support rod (17), the bottom end of the support rod (17) is located between the two outermost circular plates. The distance between the two outermost circular plates is smaller than the diameter of the support rod (17). The top and bottom of the outer circumference of the connecting pipe (18) are provided with through openings (19). The distance between the top through opening (19) and the bottom through opening (19) is greater than the thickness of the partition (4).

5. The fermentation mixture preparation equipment based on the water-soluble biotransformation of horse chestnut as described in claim 4, characterized in that: The bottom end of the connecting pipe (18) has two side rods (20) fixed to each other. Vertical rods (21) are provided on both sides of the connecting pipe (18), and the top of the vertical rods (21) is fixedly connected to the bottom surface of the partition (4). The bottom ends of the two vertical rods (21) respectively pass through the ends of the two side rods (20). An elastic element (22) is sleeved on the surface of the vertical rods (21). The top of the elastic element (22) is connected to the bottom surface of the partition (4), and the bottom end of the elastic element (22) is connected to the surface of the side rods (20).

6. The fermentation mixture preparation equipment based on the water-soluble biotransformation of horse chestnut as described in claim 5, characterized in that: The stirring rack (6) includes two side frames (23). Multiple side grooves are provided on both sides of the side frames (23). A rotating plate (24) is rotatably engaged inside the side groove. A protruding rod is provided on the top of both sides of the rotating plate (24). The rotating plate (24) rotates with the side groove of the side frame (23) using the protruding rod. A top rod (25) is provided between the two rotating plates (24) and penetrates the top of the side frame (23). A bottom rod (26) is provided at the bottom end of the top rod (25) and penetrates the bottom of the side frame (23). The top end of the bottom rod (26) is spirally engaged with the bottom end of the top rod (25). The top end of the top rod (25) corresponds to the bottom end of the connecting pipe (18).

7. The fermentation mixture preparation equipment based on the biotransformation of water-soluble horse chestnut according to claim 6, characterized in that: The top of the top rod (25) is fitted with a second spring (27), the bottom of the second spring (27) is connected to the top surface of the side frame (23), the top of the second spring (27) is connected to the top of the top rod (25), and the bottom of the top rod (25) has two supports (28) fixed relative to each other. The inner side of the rotating plate (24) is provided with a groove (29) corresponding to the support (28), and the support (28) is slidably engaged in the groove (29).

8. The fermentation mixture preparation equipment based on the biotransformation of water-soluble horse chestnut according to claim 7, characterized in that: The two rotating plates (24) on both sides of the top rod (25) are arranged opposite each other. The distance between the top ends of the two opposite inclined grooves (29) is less than the distance between their bottom ends. When the bracket (28) moves upward inside the inclined groove (29), the bracket (28) pushes the rotating plate (24) to rotate out of the side groove of the side frame (23).

9. A method for preparing fermentation mixtures based on the water-soluble biotransformation of horse chestnut, characterized in that: The preparation method uses the fermentation mixture preparation equipment based on the water-soluble biotransformation of horse chestnut as described in claim 8, and includes the following steps: S1. Obtain horse chestnuts and crush them to obtain horse chestnut seed powder; S2. Activate the lactic acid bacteria and yeast separately to obtain secondary seed culture of lactic acid bacteria and secondary seed culture of yeast, respectively. S3. The secondary seed liquid of lactic acid bacteria and secondary seed liquid of yeast obtained in S2 and the horse chestnut seed powder in S1 are mixed to obtain a mixture. The mixture is fed into the shell (1) through the feed inlet (2) and coarse fermentation is carried out at the top of the partition (4). The motor is started and the motor works to drive the partition (4) to rotate through the rotating rod (5). The partition (4) drives the support rod (17) to rotate through the circular plate at the top of the connecting structure. The support rod (17) drives the sieve plate (16) to rotate, which facilitates the preliminary mixing of the mixture. During the preliminary mixing process, urea is added into the shell (1) through the feed inlet (2) to obtain the fermentation mixture. S4. Press the top of the pressure rod (15). The pressure rod (15) drives the pressure plate (14) to press down. After the bottom surface of the pressure plate (14) is in contact with the top surface of the sieve plate (16), the pressure plate (14) uses the support rod (17) at the bottom of the sieve plate (16) to drive the partition plate (4) to move down. The rotating rod (5) at the bottom of the partition plate (4) drives the stirring frame (6) to squeeze the first spring (10) on the surface of the inner rod (9) until the bottom end of the bottom rod (26) contacts the inside of the shell (1). After the bottom surface, the reaction force of the bottom surface of the inner side of the shell (1) on the bottom rod (26) causes the bottom rod (26) to drive the top rod (25) to move upward inside the side frame (23). The upward-moving top rod (25) causes the connecting pipe (18) to move upward inside the through groove of the partition (4) until the top opening (19) moves to the top of the partition (4). The fermentation mixture at the top of the partition (4) flows to the bottom of the partition (4) through the opening (19) and the connecting pipe (18). S5. When pressing the pressure rod (15), the pressure rod (15) uses the sieve plate (16) and the support rod (17) to make the partition plate (4) move down. The partition plate (4) uses the connecting pipe (18) to squeeze and make the top rod (25) move down. During the downward movement of the top rod (25), the support (28) moves down inside the inclined groove (29). The downward movement of the support (28) makes the rotating plate (24) rotate into the side groove. When the bottom end of the bottom rod (26) contacts the inner bottom surface of the shell (1), the reaction force of the inner bottom surface of the shell (1) on the bottom rod (26) makes the bottom rod (26) drive the top rod (25) to move up. The upward movement of the top rod (25) pulls the second spring (27) at the top of the side frame (23). The upward movement of the top rod (25) makes the support (28) move in the inclined groove (29). The inner side moves upward, and the upward-moving support (28) causes the rotating plate (24) to rotate out from the inside of the side groove; the pressure applied to the top of the pressure rod (15) is released, and the first spring (10) rebounds, causing the stirring rack (6) to move upward. At this time, the bottom end of the bottom rod (26) separates from the inner bottom surface of the shell (1), and the elastic force of the second spring (27) causes the top rod (25) to move downward. The top rod (25) drives the support (28) to move downward inside the inclined groove (29). The downward-moving support (28) causes the rotating plate (24) to rotate into the inside of the side groove. Until the rotating plate (24) is completely inside the side groove, when the bottom end of the rotating rod (5) drives the stirring rack (6) to stir the fermentation mixture, the degree of mixing of the fermentation mixture inside the shell (1) is increased by the rotating plate (24), and the final product is obtained. S6. Discharge the final product through the discharge port (3).

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