Microbiological incubator

Abstract

The present application relates to the technical field of microbial culture equipment, in particular to a microbial incubator, which comprises a box body, a culture cylinder and an agitation assembly; the agitation assembly comprises an aeration seat, a rotating shaft, a paddle, a waterproof and breathable membrane, a first filter screen and a gas generating assembly; the gas generating assembly is used to fill the gas into the gas storage space, the paddle is pushed after a certain amount of gas is filled, and rotates through the rotating shaft; at this time, the position of the gas storage space will change, when the gas storage space coincides with the first through hole, the gas in the gas storage space overflows out of the aeration seat through the first through hole, and the gas bubble generated through the first filter screen agitates the culture solution to flow; the volume of the gas that can be stored in the gas storage space is greater than the volume of the gas that can overflow through the first through hole when the rotating shaft rotates one round, so as to ensure that the volumes of the gas overflowing through the plurality of first through holes are the same, realize effective and uniform dissolution of oxygen into the culture solution everywhere, and provide the same external conditions for the growth of microorganisms.

Description

Technical Field

[0001] This invention relates to the field of microbial culture equipment technology, and more specifically to a microbial incubator. Background Technology

[0002] In the culture experiment of aerobic microorganisms, the first step is to inoculate the bacterial strain into a liquid culture medium. Secondly, the culture must be carried out in an environment with sufficient oxygen to ensure that the microorganisms can metabolize and reproduce normally.

[0003] In a static liquid culture medium, sterile air is usually introduced into the bottom or side, and aeration devices such as air pumps and microporous aeration discs are used to dissolve oxygen directly into the culture medium, providing a suitable living environment for aerobic microorganisms.

[0004] However, the existing aeration device has a relatively simple structure. Multiple microporous aeration discs installed on the pipeline are dispersed among each other, and there is a certain gap between the microporous aeration discs. As a result, oxygen cannot be effectively dissolved into the culture medium in the gap. The culture medium has poor fluidity in this area, and nutrients are difficult to be transferred evenly.

[0005] Secondly, when dissolving oxygen into the culture medium through the tubing, the flow rate decreases as the gas is lost from the tubing, making it difficult to effectively and evenly dissolve oxygen throughout the culture medium, thus leading to differences in the growth rate of microorganisms. Summary of the Invention

[0006] In view of this, the purpose of the present invention is to provide a microbial incubator to solve the technical problems in the prior art, such as poor fluidity of the culture medium, difficulty in uniformly transferring nutrients, and difficulty in effectively and uniformly dissolving oxygen in various parts of the culture medium, which leads to differences in the growth rate of microorganisms.

[0007] This invention is achieved through the following technical solution:

[0008] A microbial incubator includes a box body, the top of which has a culture space, in which a culture tube is installed. The bottom inner wall of the culture tube is provided with a stirring component for promoting the equilibrium of dissolved oxygen and nutrients in the culture medium in both vertical and horizontal directions.

[0009] The agitation assembly includes an aeration seat with an inner cavity. A rotating shaft is rotatably connected to the inner cavity. Several blades are arranged in a circumferential array on the circumferential surface of the rotating shaft. The outer edge of the blades fits against the inner wall of the inner cavity, and two blades together form a gas storage space.

[0010] The top of the aeration seat is provided with several first through holes communicating with the inner cavity. A waterproof and breathable membrane is provided at the bottom opening of the first through hole, and a first filter screen is provided at the top opening of the first through hole.

[0011] It also includes a gas generating assembly for filling the cavity with gas.

[0012] Furthermore, the agitation assembly also includes a column fixedly connected to the top of the aeration seat. Limiting blocks are fixedly connected to the top and bottom of the column. A movable ring is threadedly connected to the circumferential surface of the column, and the movable ring is located between the two limiting blocks. An agitation component for agitating the culture medium is provided on the circumferential surface of the movable ring.

[0013] Furthermore, the stirring component includes an arc-shaped cover fixedly connected to the outer circumferential surface of the movable ring, the opening of the arc-shaped cover facing the first through hole, and several first through holes are all within the range of the opening of the arc-shaped cover; several turbulence plates are provided on the outer arc surface of the arc-shaped cover, and several second through holes are provided on each turbulence plate, the second through holes penetrating the turbulence plate from front to back.

[0014] Furthermore, the gas generating component includes a gas supply pipe with one end connected to the inner cavity, and the other end of the gas supply pipe passes through the aeration seat and the culture tube in sequence and extends out of the box. The end of the gas supply pipe extending out of the box is connected to an air pump and an air filter for filtering and purifying the gas supplied by the air pump.

[0015] Furthermore, a filtration space is provided at the bottom of the housing, a filter canister is fixedly connected in the filtration space, and a second filter screen is fixedly connected in the filter canister. The outer edge of the second filter screen is in contact with the inner wall of the filter canister, and the second filter screen divides the internal space of the filter canister, forming a filtration chamber and a rinsing chamber on both sides of the second filter screen respectively.

[0016] The filter chamber is connected to a first pipe and a second pipe arranged in a Y-shape on the side away from the second filter screen. The end of the first pipe extending out of the filter tank passes through the box body, the culture cylinder, the aeration seat and the rotating shaft in sequence to the bottom of the column. The bottom of the column has a notch that connects the culture cylinder and the first pipe.

[0017] The rinsing chamber is connected to a Y-shaped third pipe and a fourth pipe on the side away from the second filter screen, and each pipe is equipped with a solenoid valve.

[0018] Furthermore, a fifth pipe is connected to the second pipe, and the other end of the fifth pipe is connected to a vacuum generating component.

[0019] Furthermore, the second filter screen is installed at an angle in the filter tank, with the surface of the second filter screen facing the outlet of the first pipe, and the inlet of the second pipe is located at the lowest point between the filter tank and the second filter screen.

[0020] Furthermore, the inner wall of the filter tank located below the second filter screen is inclined away from the second filter screen to form a slope, and the liquid inlet of the fourth pipe is located at the lowest point of the slope.

[0021] Furthermore, a movable plug is slidably connected to the inner wall of the first through hole, and the movable plug is provided with several third through holes running vertically through it.

[0022] Furthermore, a storage box is fixedly connected to the bottom of the box body, a conveying pipe is connected to the storage box, and a conveying pump is connected to the other end of the conveying pipe. The output end of the conveying pump extends into the culture tube, and a nozzle is installed at the output end of the conveying pump.

[0023] The beneficial effects of this invention are as follows:

[0024] A microbial incubator of the present invention uses a gas generating component to fill the inner cavity with gas. The gas is initially stored in the gas storage space formed by the two impellers and the aeration seat. When the gas in the gas storage space reaches a certain volume, further filling will push the impellers to rotate in the aeration seat via the rotating shaft. As the impellers rotate, the position of the gas storage space changes accordingly. When the gas storage space moves to coincide with and connect with the first through hole, the gas in the gas storage space overflows from the aeration seat through the first through hole. When the gas passes through the first filter screen, it generates a large number of small bubbles. As the bubbles float from bottom to top, they stir the culture medium vertically and compress the culture medium horizontally, thereby enabling the culture medium to flow. This promotes the balance of dissolved oxygen and nutrients in the culture medium in both vertical and horizontal directions, while preventing the cultured microorganisms from accumulating at the bottom or side walls of the culture tube due to uneven nutrient composition.

[0025] Secondly, the gas volume that can be stored in the gas storage space is greater than the gas volume that can overflow through the first through hole when the shaft rotates once, so as to ensure that the gas volume overflowing through the first through hole is the same when the shaft rotates once, thereby effectively and uniformly dissolving oxygen in all parts of the culture medium, providing the same external conditions for the growth of microorganisms, and thus ensuring that the microorganisms grow at the same rate.

[0026] Other advantages, objectives, and features of the invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination, or may be learned from practice of the invention. The objectives and other advantages of the invention can be realized and obtained through the following description. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the overall structure of a microbial incubator according to the present invention.

[0028] Figure 2 This is a schematic diagram showing the connection between the aeration seat and the rotating shaft of a microbial incubator according to the present invention.

[0029] Figure 3 For the present invention Figure 2 A magnified view of a portion of point A in the middle.

[0030] Figure 4 This is a schematic diagram showing the connection between the filter tank and the second filter screen of a microbial incubator according to the present invention.

[0031] In the diagram: 1-Box body, 2-Cultivation space, 3-Cultivation cylinder, 4-Aeration seat, 5-Inner cavity, 6-Rotating shaft, 7-Impeller, 8-Gas storage space, 9-First through hole, 10-Waterproof and breathable membrane, 11-First filter screen, 12-Column, 13-Limiting block, 14-Moving ring, 15-Arc-shaped cover, 16-Break plate, 17-Second through hole, 18-Gas supply pipe, 19-Air pump, 20-Air filter, 21-Passage... Filter space, 22-Filter tank, 23-Second filter screen, 24-Filter chamber, 25-Rinsing chamber, 26-First pipe, 27-Second pipe, 28-Notch, 29-Third pipe, 30-Fourth pipe, 31-Solenoid valve, 32-Fifth pipe, 33-Vacuum generating component, 34-Inclined surface, 35-Modible plug, 36-Third through hole, 37-Storage box, 38-Transfer pipe, 39-Transfer pump, 40-Nozzle. Detailed Implementation

[0032] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0033] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.

[0034] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0035] In the above description of the present invention, it should be noted that the terms "one side," "the other side," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of the invention is conventionally placed during use. These terms are used only for the convenience of describing the present invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0036] Furthermore, terms such as "identical" do not imply that components must be absolutely identical; minor differences are permissible. The term "perpendicular" simply means that the positional relationship between components is more perpendicular than "parallel," not that the structure must be perfectly perpendicular; a slight tilt is acceptable.

[0037] Please see Figures 1 to 3 The present invention provides a technical solution: a microbial incubator, including a box body 1, a culture space 2 is provided on the top of the box body 1, a culture cylinder 3 is installed in the space, the bottom of the culture cylinder 3 is U-shaped, and an agitation component is provided on the inner wall of the bottom of the culture cylinder 3 to promote the equilibrium of dissolved oxygen and nutrients in the culture solution in both vertical and horizontal directions.

[0038] The agitation assembly includes an aeration seat 4, the shape of which matches the bottom space of the culture cylinder 3; the aeration seat 4 has an inner cavity 5, and a rotating shaft 6 is rotatably connected in the inner cavity 5. Several blades 7 are arranged in a circumferential array on the circumferential surface of the rotating shaft 6. The outer edge of the blades 7 is in contact with the inner wall of the inner cavity 5, and a gas storage space 8 is formed between every two blades 7. The gas storage space 8 is used to store the gas that is filled into the aeration seat 4 from the outside.

[0039] The top of the aeration seat 4 is provided with several first through holes 9 communicating with the inner cavity 5. A waterproof and breathable membrane 10 is provided at the bottom opening of the first through hole 9 to prevent the culture medium and microorganisms in the culture tube 3 from entering the inner cavity 5 through the first through hole 9. A first filter screen 11 is provided at the top opening of the first through hole 9. The first filter screen 11 has a large mesh size to prevent microorganisms and impurities generated during the culture process from entering the first through hole 9.

[0040] It also includes a gas generating assembly for filling the inner cavity 5 with gas.

[0041] In use, a microbial incubator of the present invention fills the inner cavity 5 with gas through the gas generating component. The gas is first stored in the gas storage space 8 formed by the two paddles 7 and the aeration seat 4. When the gas in the gas storage space 8 reaches a certain volume, the continued filling of gas will push the paddles 7 to rotate in the aeration seat 4 through the rotating shaft 6. When the paddles 7 rotate, the position of the gas storage space 8 will change accordingly. When the gas storage space 8 moves to coincide with and communicate with the first through hole 9, the gas in the gas storage space 8 overflows out of the aeration seat 4 through the first through hole 9. When the gas passes through the first filter screen 11, it generates a large number of small bubbles. As the bubbles float from bottom to top, they stir the culture medium in the vertical direction and squeeze the culture medium in the horizontal direction, thereby enabling the culture medium to flow. This promotes the balance of dissolved oxygen and nutrients in the culture medium in both the vertical and horizontal directions, while preventing the cultured microorganisms from accumulating on the bottom or side wall of the culture cylinder 3 due to uneven nutrient composition.

[0042] Secondly, the gas volume that can be stored in the gas storage space 8 is greater than the gas volume that can overflow through the first through hole 9 when the rotating shaft 6 rotates once, so as to ensure that the gas volume overflowing through the first through hole 9 is the same when the rotating shaft 6 rotates once, thereby effectively and uniformly dissolving oxygen in all parts of the culture medium, providing the same external conditions for the growth of microorganisms, and thus ensuring that the microorganisms grow at the same rate.

[0043] Please see Figure 1 In this embodiment, the stirring assembly further includes a column 12 fixedly connected to the top of the aeration seat 4. Limiting blocks 13 are fixedly connected to the top and bottom of the column 12. A movable ring 14 is threadedly connected to the circumferential surface of the column 12, and the movable ring 14 is located between the two limiting blocks 13. A stirring component for stirring the culture medium is provided on the circumferential surface of the movable ring 14.

[0044] The stirring component includes an arc-shaped cover 15 fixedly connected to the outer circumferential surface of the movable ring 14. The opening of the arc-shaped cover 15 faces the first through hole 9, and several of the first through holes 9 are within the opening range of the arc-shaped cover 15. Several turbulence plates 16 are provided on the outer arc surface of the arc-shaped cover 15. Several second through holes 17 are provided on each turbulence plate 16, and the second through holes 17 penetrate the turbulence plate 16 from front to back.

[0045] In this design, as the bubbles float upwards, a large number of bubbles enter the arc-shaped cover 15 from below, thereby pushing the arc-shaped cover 15 upwards along the column 12 via the movable ring 14. Gas is intermittently injected into the inner cavity 5 by the gas generating component, thereby driving the arc-shaped cover 15 to reciprocate in the vertical direction (after the arc-shaped cover 15 moves upwards, it descends under the influence of gravity, and a spring (not shown) is provided between the arc-shaped cover 15 and the limiting block 13 at the top. The arc-shaped cover 15 squeezes the spring during its upward movement, and can better move and reset under the force generated by the deformation of the spring). This agitates the culture medium, thereby further promoting the vertical equilibrium of dissolved oxygen and nutrients in the culture medium.

[0046] Secondly, since the movable ring 14 is threadedly connected to the column 12, when the arc-shaped cover 15 moves upward, the movable ring 14 and the column 12 rotate circumferentially, thereby driving the arc-shaped cover 15 to rotate. The rotation of the arc-shaped cover 15 drives the baffle 16 to rotate, thereby further promoting the dissolved oxygen and nutrients in the culture medium to tend to be balanced in the horizontal direction. In addition, during the rotation of the arc-shaped cover 15, the culture medium flows through the second through hole 17, which can further improve the stirring effect on the culture medium.

[0047] Finally, by placing the arc-shaped cover 15 over the trajectory of the bubble, the gas overflow from the culture medium surface can be slowed down, thereby increasing the contact time between the gas and the culture medium, allowing the gas to dissolve better in the culture medium, and reducing the loss caused by gas escape due to supersaturation of dissolved oxygen in the culture medium.

[0048] Please see Figure 1 In this embodiment, the gas generating component includes a gas supply pipe 18 with one end connected to the inner cavity 5, and the other end of the gas supply pipe 18 passes through the aeration seat 4 and the culture tube 3 in sequence and extends out of the box body 1. The end of the gas supply pipe 18 extending out of the box body 1 is connected to an air pump 19 and an air filter 20 for filtering and purifying the gas supplied by the air pump 19.

[0049] In this scheme, gas can be injected into the inner cavity 5 by the air pump 19 and the air supply pipe 18. The air filter 20 can filter out impurities and bacteria in the injected gas, thereby providing suitable growth conditions for microorganisms.

[0050] Please see Figure 1 and Figure 4 In this embodiment, a filtration space 21 is provided at the bottom of the housing 1. A filter tank 22 is fixedly connected in the filtration space 21. A second filter screen 23 is fixedly connected in the filter tank 22. The outer edge of the second filter screen 23 is attached to the inner wall of the filter tank 22, and the second filter screen 23 divides the internal space of the filter tank 22. A filter chamber 24 and a rinsing chamber 25 are formed on both sides of the second filter screen 23, respectively.

[0051] The filter chamber 24, away from the second filter screen 23, is connected to a first pipe 26 and a second pipe 27 arranged in a Y-shape. The first pipe 26 extends out of the filter tank 22 and passes through the box body 1, the culture cylinder 3, the aeration seat 4 and the rotating shaft 6 in sequence to the bottom of the column 12. The bottom of the column 12 has a notch 28 connecting the culture cylinder 3 and the first pipe 26.

[0052] The flushing chamber 25 is connected to a Y-shaped third pipe 29 and a fourth pipe 30 on the side away from the second filter screen 23, and each pipe is equipped with a solenoid valve 31.

[0053] In this scheme, after the microbial culture is completed, the second pipe 27 and the third pipe 29 are closed by controlling the multiple solenoid valves 31 respectively, while the first pipe 26 and the fourth pipe 30 are opened. At this time, the turbid liquid containing microorganisms enters the inlet of the first pipe 26 through the notch 28 and is introduced into the filter chamber 24 through the first pipe 26. The turbid liquid entering the filter chamber 24 is filtered by the second filter screen 23, so that impurities with a volume larger than the filter holes of the second filter screen 23 remain on the left side of the second filter screen 23, while microorganisms with a volume smaller than the filter holes of the second filter screen 23 pass through the second filter screen 23 and are discharged from the filter tank 22 through the fourth pipe 30. Thus, impurities with a volume larger than microorganisms are filtered out, and coarse filtration of the turbid liquid is achieved. After coarse filtration, it can be introduced into the filtration mechanism of this invention (using a filter screen with a larger mesh size) for secondary filtration to remove impurities with a volume smaller than microorganisms. Its working principle and operation steps are similar to the first filtration step, and will not be described in detail here.

[0054] Next, the first pipe 26 and the fourth pipe 30 are closed by controlling the multiple solenoid valves 31 respectively, while the second pipe 27 and the third pipe 29 are opened. Clean water is added to the flushing chamber 25 through the third pipe 29 to flush out the impurities on the left side of the second filter screen 23 and those attached to it from the second pipe 27, thereby cleaning the second filter screen 23, reducing the possibility of clogging, and ensuring filtration efficiency and effect. Furthermore, the multiple pipes work together to form a cross-path, achieving integrated filtration and cleaning, reducing the need for subsequent cleaning of the second filter screen 23. Finally, the present invention can control the opening or closing of the corresponding solenoid valves 31 by setting the time and the volume of the filtered turbid liquid, making the filtration of microorganisms intelligent, so that the filtration work can be completed automatically, quantitatively, and on a timed basis, eliminating the trouble of manual operation.

[0055] Please see Figure 4 In this embodiment, a fifth pipe 32 is connected to the second pipe 27. The other end of the fifth pipe 32 is connected to a vacuum generating component 33, which is a vacuum pump. A waterproof and breathable membrane is provided in the fifth pipe 32 to prevent water from entering the vacuum pump and causing damage to the vacuum pump.

[0056] In this scheme, when rinsing the second filter screen 23, after adding clean water to the rinsing space through the third pipe 29, the vacuum generating component 33 and the fifth pipe 32 work together to extract air from the filter tank 22. At this time, the pressure in the filter tank 22 decreases, thereby increasing the flow rate of clean water flowing out of the filter tank 22 through the third pipe 29. This can better pump out the impurities on the left side of the second filter screen 23 and those attached to it, ensuring the rinsing effect of the second filter screen 23. The same vacuum generating component 33 can also be installed on the fourth pipe 30, which is beneficial to accelerate the separation of microorganisms and impurities.

[0057] Please see Figure 4 In this embodiment, the second filter screen 23 is installed at an angle in the filter tank 22, with the surface of the second filter screen 23 facing the liquid outlet of the first pipe 26, and the liquid inlet of the second pipe 27 is located at the lowest point between the filter tank 22 and the second filter screen 23.

[0058] In this solution, by installing the second filter screen 23 at an angle in the filter tank 22, the contact area between the second filter screen 23 and the turbid liquid is increased, thereby improving the filtration efficiency of the turbid liquid. Furthermore, the second filter screen 23 is angled at the inlet of the second pipe 27, which can guide the movement direction of impurities during cleaning, so that the impurities are moved more completely to the inlet of the second pipe 27 and finally discharged from the filter tank 22 through the second pipe 27.

[0059] Please see Figure 4 In this embodiment, the inner wall of the filter tank 22 located below the second filter screen 23 is inclined away from the second filter screen 23 to form a slope 34, and the liquid inlet of the fourth pipe 30 is located at the lowest point of the slope 34.

[0060] In this scheme, the inclined surface 34 allows the microorganisms filtered by the second filter screen 23 to move smoothly along the inclined surface 34 to the inlet of the fourth pipe 30, and to be transferred out of the filter tank 22 relatively completely through the fourth pipe 30.

[0061] Please see Figure 3 In this embodiment, a movable plug 35 is slidably connected to the inner wall of the first through hole 9, and the movable plug 35 is provided with a plurality of vertically penetrating third through holes 36.

[0062] In this design, the top of the aeration seat 4 is recessed downwards to form a concave surface. Gas is intermittently injected into the inner cavity 5 through the gas generating component. The third through hole 36 ensures that the gas can be smoothly discharged from the first through hole 9. Since the movable plug 35 blocks the space above the waterproof and breathable membrane 10, the speed at which the gas is discharged from the first through hole 9 is slowed down. At this time, the movable plug 35 is pushed by the gas to move towards the first filter screen 11 and impacts the first filter screen 11 to generate vibration. This allows the microorganisms and turbid liquid in the culture tube 3 to be more completely introduced into the filter tank 22 through the first pipe 26 for impurity separation after the culture is completed. This avoids the accumulation of a lot of impurities on the top of the aeration seat 4 and facilitates the cleaning of the inside of the culture tube 3.

[0063] Please see Figure 1 In this embodiment, a storage box 37 is fixedly connected to the bottom of the box 1, a conveying pipe 38 is connected to the storage box 37, and a conveying pump 39 is connected to the other end of the conveying pipe 38. The output end of the conveying pump 39 extends into the culture tube 3, and a nozzle 40 is installed at the output end of the conveying pump 39.

[0064] In this scheme, the storage box 37 contains culture medium. The delivery pump 39 on the delivery pipe 38 is turned on to extract the culture medium from the storage box 37 and add it to the culture cylinder 3 through the nozzle 40. After the culture cylinder 3 is filled with culture medium, microorganisms can be added to the culture cylinder 3 from the other side of the box 1 in the same way as described above. This allows control of the input ratio of microorganisms to culture medium, providing a suitable living environment for the growth of microorganisms and increasing the survival rate of microorganisms.

[0065] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A microbial incubator, comprising a chamber (1), characterized in that: The top of the box (1) is provided with a culture space (2), in which a culture tube (3) is installed. The bottom inner wall of the culture tube (3) is provided with a stirring component to promote the equilibrium of dissolved oxygen and nutrients in the culture medium in both vertical and horizontal directions. The agitation assembly includes an aeration seat (4), which has an inner cavity (5). A rotating shaft (6) is rotatably connected in the inner cavity (5). Several blades (7) are arranged in a circular array on the circumferential surface of the rotating shaft (6). The outer edge of the blades (7) is in contact with the inner wall of the inner cavity (5), and two blades (7) together form a gas storage space (8). The top of the aeration seat (4) is provided with a plurality of first through holes (9) communicating with the inner cavity (5). A waterproof and breathable membrane (10) is provided at the bottom opening of the first through hole (9), and a first filter screen (11) is provided at the top opening of the first through hole (9). It also includes a gas generating assembly for filling the cavity (5) with gas.

2. A microbial incubator according to claim 1, characterized in that: The stirring assembly also includes a column (12) fixedly connected to the top of the aeration seat (4). Limiting blocks (13) are fixedly connected to the top and bottom of the column (12). A movable ring (14) is threadedly connected to the circumferential surface of the column (12), and the movable ring (14) is located between the two limiting blocks (13). A stirring component for stirring the culture medium is provided on the circumferential surface of the movable ring (14).

3. A microbial incubator according to claim 2, characterized in that: The stirring component includes an arc-shaped cover (15) fixedly connected to the outer circumferential surface of the movable ring (14). The opening of the arc-shaped cover (15) faces the first through hole (9), and several of the first through holes (9) are within the opening range of the arc-shaped cover (15). Several turbulence plates (16) are provided on the outer arc surface of the arc-shaped cover (15). Several second through holes (17) are provided on each turbulence plate (16), and the second through holes (17) pass through the turbulence plate (16) from front to back.

4. A microbial incubator according to claim 1, characterized in that: The gas generating assembly includes a gas supply pipe (18) with one end connected to the inner cavity (5), and the other end of the gas supply pipe (18) passes through the aeration seat (4) and the culture tube (3) in sequence and extends out of the box body (1). The end of the gas supply pipe (18) extending out of the box body (1) is connected to an air pump (19) and an air filter (20) for filtering and purifying the gas supplied by the air pump (19).

5. A microbial incubator according to claim 2, characterized in that: The bottom of the housing (1) is provided with a filter space (21), a filter tank (22) is fixedly connected in the filter space (21), a second filter screen (23) is fixedly connected in the filter tank (22), the outer edge of the second filter screen (23) is in contact with the inner wall of the filter tank (22), and the second filter screen (23) divides the internal space of the filter tank (22), forming a filter chamber (24) and a rinsing chamber (25) on both sides of the second filter screen (23); The filter chamber (24) away from the second filter screen (23) is connected to a first pipe (26) and a second pipe (27) arranged in a Y shape. The first pipe (26) extends out of the filter tank (22) and passes through the box body (1), the culture tube (3), the aeration seat (4) and the rotating shaft (6) in sequence to the bottom of the column (12). The bottom of the column (12) has a notch (28) connecting the culture tube (3) and the first pipe (26). The flushing chamber (25) is connected to a Y-shaped third pipe (29) and a fourth pipe (30) on the side away from the second filter screen (23), and each pipe is equipped with a solenoid valve (31).

6. A microbial incubator according to claim 5, characterized in that: A fifth pipe (32) is connected to the second pipe (27), and the other end of the fifth pipe (32) is connected to a vacuum generating component (33).

7. A microbial incubator according to claim 5, characterized in that: The second filter screen (23) is installed at an angle in the filter tank (22), with the surface of the second filter screen (23) facing the outlet of the first pipe (26), and the inlet of the second pipe (27) is located at the lowest point between the filter tank (22) and the second filter screen (23).

8. A microbial incubator according to claim 5, characterized in that: The inner wall of the filter tank (22) located below the second filter screen (23) is inclined away from the second filter screen (23) to form a slope (34), and the liquid inlet of the fourth pipe (30) is located at the lowest point of the slope (34).

9. A microbial incubator according to claim 1, characterized in that: A movable plug (35) is slidably connected to the inner wall of the first through hole (9), and the movable plug (35) is provided with a plurality of third through holes (36) that extend vertically through the hole.

10. A microbial incubator according to claim 1, characterized in that: A storage box (37) is fixedly connected to the bottom of the box (1). A delivery pipe (38) is connected to the storage box (37). A delivery pump (39) is connected to the other end of the delivery pipe (38). The output end of the delivery pump (39) extends into the culture tube (3), and a nozzle (40) is installed at the output end of the delivery pump (39).

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