Infection-proof stem cell culture protection device

Abstract

The application discloses an anti-infection stem cell culture protection device, and relates to the technical field of stem cell culture. The device comprises a culture box, a fixing device, a culture dish, an opening and closing protection rotating mechanism and an integral electron microscope. The fixing device is arranged on the culture box and used for providing a culture environment for stem cell culture. The culture dish is arranged in the culture box and used for containing stem cell culture solution and stem cell samples for culture. The opening and closing protection rotating mechanism is arranged in the culture box and the culture dish is arranged on the opening and closing protection rotating mechanism. The device is integrated with a ring-shaped automatic opening and closing mechanism. All the operations of opening and closing the culture dish are completed in the closed and sterile culture box. Manual opening and closing operations are not needed. Bacteria and fungi brought by external airflow are isolated. The integral electron microscope is arranged on the culture box. In-situ imaging is realized without opening the culture dish by the silicon nitride observation window. The sample open transport process is completely cancelled. Cross contamination between different samples is avoided. The survival rate of stem cells is greatly improved.

Description

Technical Field

[0001] This invention relates to the field of stem cell culture technology, and in particular to an infection-preventing protective device for stem cell culture. Background Technology

[0002] The in vitro culture of stem cells requires an extremely strict sterile environment. Once the cells come into contact with external bacteria or fungi, they will be contaminated, directly causing the entire batch of samples to be scrapped and significantly increasing the research and experimental costs.

[0003] However, in practical applications, there are still some unresolved issues. The following are some common problems with stem cell culture devices: Traditional stem cell culture processes have two high risks of contamination: First, after the culture dish is placed in the incubator, the lid must be opened by hand to expose the stem cells in the culture environment. During this process, bacteria and fungi carried by the outside air flow into the incubator, causing stem cell contamination. Second, the culture dish must be removed and transported to the electron microscope equipment for observation. The lid of the dish is open throughout the transport process, which makes cross-contamination very easy to occur. Summary of the Invention

[0004] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0005] In view of the problems existing in the above and / or existing infection-proof stem cell culture protective devices, the present invention is proposed.

[0006] Therefore, the problem to be solved by this invention is how to solve the problem of stem cell contamination caused by manually opening the container and lifting the lid, which introduces bacteria from the outside airflow, and the problem of cross-contamination caused by the need to remove the culture dish and transport it to an external microscope.

[0007] To solve the above-mentioned technical problems, the present invention provides the following technical solution: an infection-proof stem cell culture protective device, comprising: a culture box, fixedly installed, for providing culture environment conditions for stem cell culture; the culture box includes a box body and a culture dish, disposed within the box body, for holding stem cell culture medium and stem cell samples for culture; an opening and closing protective rotating mechanism, installed within the box body, with the culture dish disposed thereon, for supporting the placement of the culture dish and simultaneously realizing the opening and closing of the culture dish body and the dish lid; the opening and closing protective rotating mechanism includes: a rotating component, installed on the box body; circular tubes, symmetrically distributed in a ring array and installed on the rotating component; and a cylindrical body, rotatably sleeved on one of the circular tubes. End; Support component, fixed to the surface of the cylinder, used for placing and supporting the culture dish; Dish body limiting and unlocking component, installed on the support component and the cylinder, used for limiting and unlocking the culture dish body; Dish lid lifting and limiting unlocking component, installed on the support component and the cylinder, used for limiting and unlocking the culture dish lid, and controlling the opening and closing between the dish lid and the dish body; Trigger component, installed on the cylindrical tube and the dish lid lifting and limiting unlocking component, and cooperates with the dish body limiting and unlocking component and the dish lid lifting and limiting unlocking component; Drive component, installed on the box body, and cooperates with the trigger component; Electron microscope, installed through the box body, used for in-situ observation and detection of stem cell samples cultured inside the box body.

[0008] As a preferred embodiment of the infection-preventing stem cell culture protective device of the present invention, the culture dish includes: a dish body placed on a support; a dish lid covering the dish body; a silicon nitride window embedded in the dish lid; a locking hole one formed on the dish body and adapted to engage with the dish body limiting and unlocking component; and a locking hole two formed on the dish lid and adapted to engage with the dish lid lifting and lowering limiting and unlocking component.

[0009] As a preferred embodiment of the infection-preventing stem cell culture protective device of the present invention, the rotating component includes: a rotating rod that rotates vertically within the housing; a sprocket fixedly mounted on both ends of the two rotating rods; a chain that drives the sprocket surfaces on the two rotating rods; a connecting plate fixed to the chain, with a round tube passing through and fixed to the end of the connecting plate away from the chain; and a motor installed on one side of the housing, with its output shaft fixed to one end of the rotating rod.

[0010] As a preferred embodiment of the infection-preventing stem cell culture protective device of the present invention, the rotating component further includes: a reinforcing rod, both ends of which are fixed to two horizontally adjacent connecting plates; a short rod, fixed to the inner wall of the box; and a baffle, fixed to one end of the short rod and cooperating with one side of the chain.

[0011] As a preferred embodiment of the infection-preventing stem cell culture protective device of the present invention, the support member includes: a connecting plate, one end of which is fixed to the surface of the cylinder; a fixing rod, one end of which is fixed to the other end of the connecting plate; a support plate, which is fixed to the end of the fixing rod away from the connecting plate; a placement groove is provided on the top of the support plate, and a dish limiting and unlocking component is installed on the support plate and the cylinder.

[0012] As a preferred embodiment of the infection-preventing stem cell culture protective device of the present invention, the triggering element includes: a round rod that slides through the round tube and cooperates with the dish lid lifting limit unlocking component; a conical ring that is rotatably sleeved on one end of the round rod and cooperates with the dish body limiting unlocking component; a guide strip that slides through the round tube and is fixed to the surface of the round rod; a synchronization plate that is fixed to one end of the round rod and cooperates with the driving component; and steel balls that are rotatably embedded in a circumferential array on both sides of one end of the synchronization plate and cooperate with the driving component.

[0013] As a preferred embodiment of the infection-preventing stem cell culture protective device of the present invention, the driving component includes: an electric telescopic rod, one end of which is fixed to the inner wall of the box; and a driving frame, which is fixed to the other end of the electric telescopic rod and cooperates with the synchronization plate and the steel ball respectively.

[0014] As a preferred embodiment of the infection-preventing stem cell culture protective device of the present invention, the dish body limiting and unlocking component includes: a locking plate, which slides through both sides of the support plate and has one end engaged with a locking hole; a transmission plate, which slides through the cylinder and has one end engaged with a conical ring; a round block, which is fixedly sleeved on the surface of the transmission plate; a spring, which is sleeved on the surface of the transmission plate and has its two ends fixed between the surface of the cylinder and the surface of the round block, respectively; a spring piece, whose two ends are fixed between the surface of the locking plate and the surface of the support plate; and a trigger hole, which is opened at one end of the locking plate and engages with one end of the transmission plate.

[0015] As a preferred embodiment of the infection-preventing stem cell culture protective device of the present invention, the dish lid lifting and limiting unlocking component includes: a Z-shaped plate, which slides through the cylinder; a locking block, which slides through one end of the Z-shaped plate and whose end is adapted to engage with a second locking hole; a guide frame, which is disposed in the inner cavity of the cylinder and fixed to the other end of the Z-shaped plate; a slider, which slides within the guide frame; a pulley, which rotates on the slider; a drive plate, which is rotatably connected to the end of a round rod away from the synchronization plate via a bearing; a guide hole, which is opened on the drive plate and the pulley slides within the guide hole; a second spring, which is sleeved on the surface of the Z-shaped plate and whose two ends are respectively fixed to the surface of the cylinder and the surface of the Z-shaped plate; a roller, which rotates on the drive plate; and a track is provided on the cylinder and the roller slides within the track.

[0016] As a preferred embodiment of the infection-preventing stem cell culture protective device of the present invention, the dish cover lifting limit unlocking component further includes: a third spring, disposed within the guide frame, with both ends fixed between the inner wall of the guide frame and the surface of the slider; a connecting rope, slidingly passing through the Z-shaped plate, with both ends fixed between the surface of the locking block and the surface of the slider; a second spring, with both ends fixed between the surface of the Z-shaped plate and the surface of the locking block; a first guide wheel, rotating at one end of the Z-shaped plate; a second guide wheel, rotating on the Z-shaped plate, with the connecting rope cooperating with the first guide wheel and the second guide wheel respectively.

[0017] The beneficial effects of this invention are as follows: 1. Fully automatic lid opening inside the chamber + built-in in-situ observation, doubly blocking the path of bacterial contamination. The equipment integrates a ring-shaped automatic opening and closing mechanism. All lid opening and closing operations are completed inside the sealed sterile chamber, eliminating the need for manual opening and operation. It isolates bacteria and fungi brought in by external airflow. Combined with an integrated electron microscope, it achieves in-situ imaging without opening the lid by relying on the silicon nitride observation window, completely eliminating the sample open transport process, preventing cross-contamination between different samples, greatly improving the survival rate of stem cells, and reducing the waste of experimental consumables and the cost of repeated experiments.

[0018] 2. The multi-station cyclic linkage structure simplifies operation and is suitable for batch cell culture. The chain ring intermittent conveyor can place multiple sets of culture dishes at the same time, and the workstations are automatically rotated for observation. There is no need for manual repeated picking and placing, opening and closing of the box door, which reduces the risk of contamination caused by manual intervention. A single electric telescopic rod can simultaneously complete the entire sequence of actions of locking the dish and clamping and lifting the dish lid. It is suitable for the needs of laboratory operations for simultaneous culture and timed observation of large batches of stem cells, which significantly improves the degree of automation and detection efficiency. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 The overall three-dimensional protective device for stem cell culture to prevent infection Figure 1 .

[0021] Figure 2 The overall three-dimensional protective device for stem cell culture to prevent infection Figure 2 .

[0022] Figure 3 Partial plan view of the protective device for stem cell culture to prevent infection.

[0023] Figure 4 A partial three-dimensional diagram of a protective device for stem cell culture to prevent infection.

[0024] Figure 5 A partial structural breakdown of a protective device for stem cell culture designed to prevent infection.

[0025] Figure 6 A three-dimensional cross-sectional view of a protective device for stem cell culture designed to prevent infection.

[0026] Figure 7 Protective devices for stem cell culture to prevent infection Figure 6 Enlarged view of point A in the middle.

[0027] Figure 8 Protective devices for stem cell culture to prevent infection Figure 6 Enlarged view of section B in the middle.

[0028] Figure 9 Protective devices for stem cell culture to prevent infection Figure 6 Enlarged view of point C in the middle.

[0029] Figure 10 A partial sectional perspective view of the cylinder of a stem cell culture protective device designed to prevent infection.

[0030] In the diagram: 1. Incubator; 11. Chamber; 2. Petri dish; 21. Dish body; 22. Dish lid; 23. Silicon nitride window; 24. Locking hole one; 25. Locking hole two; 3. Opening and closing protective rotating mechanism; 31. Rotating component; 32. Round tube; 33. Cylinder; 34. Support component; 35. Dish body limiting and unlocking component; 36. Dish lid lifting and lowering limiting and unlocking component; 37. Trigger component; 38. Drive component; 4. Electron microscope; 311. Rotating rod; 312. Sprocket; 313. Chain; 314. Connecting plate; 315. Motor; 316. Reinforcing rod; 317. Short rod; 318. Baffle; 341. Connecting plate; 342. Fixing rod; 343. Support plate; 351. Locking plate; 352. Transmission plate; 353. Round block; 354. Spring 1; 355. Spring piece 1; 356. Trigger hole; 357. Guide groove; 358. Guide block; 361. Z-shaped plate; 362. Locking block; 363. Guide frame; 364. Slider; 365. Pulley; 366. Drive plate; 367. Guide hole; 368. Spring 2; 369. Roller; 3610. Spring 3; 3611. Connecting rope; 3612. Spring piece 2; 3613. Guide wheel 1; 3614. Guide wheel 2; 3615. Limiting groove; 3616. Limiting block; 331. Track; 371. Round rod; 372. Conical ring; 373. Guide bar; 374. Synchronization plate; 375. Steel ball; 381. Electric telescopic rod; 382. Drive frame. Detailed Implementation

[0031] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0032] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0033] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0034] Example 1, referring to Figures 1-4 This is the first embodiment of the present invention. This embodiment provides an infection-proof stem cell culture protective device, which includes a culture box 1, a culture dish 2, an opening and closing protective rotating mechanism 3, and an electron microscope 4.

[0035] Specifically, the incubator 1 is fixedly installed and used to provide culture environment conditions for stem cell culture; it includes a box body 11. The incubator 1 is an existing mature device that can stably provide the constant temperature, constant humidity, sterile, and gas ratio culture environment required for stem cell growth. The box body 11 is an integral sealed load-bearing structure. All of these are existing technologies, which can be clearly understood by those skilled in the art, and will not be described in detail here.

[0036] Specifically, the culture dish 2 is placed inside the box 11 and is used to hold stem cell culture medium and stem cell samples for culturing.

[0037] Specifically, the opening and closing protective rotating mechanism 3 is installed inside the box 11, and the culture dish 2 is placed on it to support the culture dish 2. At the same time, it enables the opening and closing of the dish body 21 and the lid 22 on the culture dish 2. This avoids the need for manual operation after the culture dish 2 is placed in the incubator 1, which could cause backflow of air and contaminate the stem cells. It can also prevent external bacteria and fungi from invading the stem cells when the culture sample is transferred to the electron microscope 4 for observation, thus avoiding cross-contamination of the stem cells by the intrusion of bacteria and fungi when the culture dish is open.

[0038] The opening and closing protective rotating mechanism 3 includes: a rotating component 31, mounted on the housing 11; several round tubes 32, symmetrically distributed in a ring array and mounted on the rotating component 31; several cylindrical bodies 33, correspondingly rotatably sleeved on one end of the round tubes 32; and several supporting components 34, correspondingly fixed to the surface of the cylindrical bodies 33, for supporting the placement of the culture dish 2.

[0039] A plate-mounted limiting and unlocking component 35, installed on the support 34 and the cylinder 33, is used for limiting and unlocking the plate body 21 on the culture dish 2; a plate-lid lifting limiting and unlocking component 36, installed on the support 34 and the cylinder 33, is used for limiting and unlocking the plate-lid 22 on the culture dish 2, and for controlling the opening and closing between the plate-lid 22 and the plate body 21; several trigger components 37 are installed on the circular tube 32 and the plate-lid lifting limiting and unlocking component 36 respectively, and are used in conjunction with the plate-mounted limiting and unlocking component 35 and the plate-lid lifting limiting and unlocking component 36. The limiting unlocking component 36 cooperates with the drive component 38, which is installed on the box 11 and cooperates with the trigger component 37. The control rotating component 31 rotates intermittently, causing multiple support components 34 to rotate cyclically, placing multiple culture dishes 2 on the support components 34. By controlling the drive component 38 to run the trigger component 37, the dish body limiting unlocking component 35 and the dish lid lifting limiting unlocking component 36 are driven to move, thereby realizing the fixing of the dish body 21, the fixing of the dish lid 22, and the opening of the dish lid 22 in sequence. When it is necessary to remove it, the operation can be reversed.

[0040] The innovative core of the mechanism is the opening and closing protective rotation mechanism 3, which is integrated and installed inside the box 11. The culture dish 2 is directly mounted on top of the mechanism, realizing fully automatic closed-station rotation, limiting and fixing of the dish body 21, and automatic opening and closing of the dish lid 22. The entire process does not require manual opening of the box or manual touching of the lid. The mechanism is specifically composed of a rotating component 31, multiple sets of circular tubes 32, a cylinder 33, a support component 34, a dish body limiting and unlocking component 35, a dish lid lifting and limiting unlocking component 36, a trigger component 37, and a driving component 38. It adopts a multi-station ring array arrangement, which can simultaneously carry multiple sets of culture dishes 2 to realize continuous rotation culture and observation operations.

[0041] When the mechanism is running, the drive component 38 provides active power, which works in conjunction with the trigger component 37 to link two sets of limit unlocking structures. It strictly follows the sequence of "the dish body 21 locks first, the dish cover 22 locks then, and the dish cover 22 is then lifted to open". After the culture and observation are completed, the reverse reset is closed and unlocked. The whole process is completed in the sealed and sterile environment of the box 11. At the same time, the electron microscope 4 is installed through the box 11, which can directly perform in-situ microscopic observation of the stem cell samples after the box is opened. The sample transportation process is completely eliminated, and the pollution from transportation and open exposure is eliminated from the source, which greatly improves the cleanliness and survival rate of stem cell culture.

[0042] Specifically, the electron microscope 4 is mounted throughout the chamber 11 and is used for in-situ observation and detection of stem cell samples cultured inside the chamber 11. There is no need to open the culture dish 2 to remove it and transfer it to an independent observation area. The entire observation operation is completed in the closed and clean environment of the culture chamber 1, which further reduces the probability of infection and contamination during sample transfer and observation. The electron microscope 4 is existing technology, and the working principle of this part is also existing technology, which can be clearly understood by those skilled in the art, and will not be described in detail here.

[0043] Example 2, refer to Figures 2-6 This is the second embodiment of the present invention, which is based on the previous embodiment.

[0044] Specifically, the culture dish 2 is adapted to the fully automatic limiting, opening and closing, and in-situ observation conditions of this device. The culture dish 2 includes: a dish body 21, which is placed on the support 34 and is used to hold stem cell culture medium and cell samples; a dish lid 22, which is placed on the dish body 21 and is normally placed on top of the dish body 21 to achieve airtight dust and bacteria prevention; and a silicon nitride window 23, which is embedded in the dish lid 22 and is used to allow the electron beam of the electron microscope 4 to penetrate, ensuring the clarity of observation. The silicon nitride material has the advantages of high electron beam penetration, high light transmittance, good biocompatibility, and sterility without precipitation. Under the premise of ensuring open culture, it meets the penetration imaging requirements of the electron microscope 4 and ensures the clarity of observation.

[0045] A sealing treatment is applied between the dish body 21 and the dish lid 22. A locking hole 24 is provided on the dish body 21 and is adapted to engage with the dish body limiting unlocking component 35 to achieve lateral fixation of the dish body 21. Two locking holes 24 are symmetrically arranged on one dish body 21. A locking hole 25 is provided on the dish lid 22 and is adapted to engage with the dish lid lifting limiting unlocking component 36. Two locking holes 25 are symmetrically arranged on one dish lid 22 to achieve clamping and limiting of the dish lid 22. The double-sided symmetrical hole structure ensures uniform force, smooth lifting and lowering, and no tilting or jamming, and is adapted to automated opening and closing actions.

[0046] When the equipment is in standby culture, the end of the dish body limiting unlocking component 35 is inserted into the first locking hole 24, which firmly locks the dish body 21 onto the support component 34, preventing the dish body 21 from shifting during the opening process. Then, the lifting limiting unlocking component of the dish cover 22 is inserted into the second locking hole 25, which clamps and fixes the dish cover 22. The dish cover 22 is then lifted upward to open the cover. The dish cover 22 is fully open and closed, and the sample is in a closed and sterile culture state. The rotating component 31 is controlled to run. After reaching the observation position, during observation, the electron beam of the electron microscope 4 penetrates the silicon nitride window 23 to complete the imaging detection.

[0047] Specifically, the rotating component 31 is the core power assembly for multi-station cyclic conveying inside the housing 11. The rotating component 31 includes: two rotating rods 311, symmetrically distributed and rotating at the top and bottom of the housing 11; four sprockets 312, symmetrically distributed and fixedly sleeved at both ends of the two rotating rods 311; and two chains 313, which are driven and sleeved on the surfaces of the sprockets 312 on the two rotating rods 311. The synchronous transmission structure of the double rotating rods 311, double sprockets 312, and chains 313 ensures smooth operation, large load capacity, and high positioning accuracy, forming two sets of... A vertical synchronous ring transmission circuit; several connecting plates 314 are fixed to the chain 313 in a ring array, and a circular tube 32 is fixed through and fixed to the end of the connecting plate 314 away from the chain 313. Multiple connecting plates 314 and circular tubes 32 realize multi-station array arrangement; a motor 315 is installed on one side of the box 11, and the output shaft is fixed to one end of the rotating rod 311. The motor 315 is a geared self-locking motor, which provides intermittent rotation power for the overall chain 313 conveying, realizes the cyclical conveying of multiple sets of culture dishes 2, and meets the operation requirements of batch observation.

[0048] The motor 315 operates intermittently, driving the rotating rod 311 and sprocket 312 to rotate at a constant speed, which in turn drives two sets of chains 313 to rotate synchronously in a circular motion. The chains 313 drive all connecting plates 314, round tubes 32, cylinders 33, and the culture dishes 2 above to move cyclically along the circular trajectory. When a set of culture dishes 2 reaches the microscope observation position, the motor 315 immediately stops and locks. This is existing technology, which is clearly known to those skilled in the art and will not be elaborated here. This ensures accurate positioning of the work position. After the observation process is completed, the motor 315 starts again to switch to the next work position. The synchronous transmission of the double chains 313 can effectively balance the load and avoid unilateral tilting and shaking.

[0049] Specifically, the rotating component 31 also includes: several reinforcing rods 316, each fixed at both ends to two laterally adjacent connecting plates 314. The reinforcing rods 316 connect the dispersed independent connecting plates 314 into an overall frame, significantly improving the overall rigidity of the ring conveyor mechanism, effectively suppressing load swaying, start-stop vibration, and cantilever deformation, and ensuring the structural stability of long-term high-frequency operation; two short rods 317, symmetrically distributed and fixed to the inner wall of the housing 11; and two baffles 318, symmetrically distributed and correspondingly fixed to one end of the short rods 317, cooperating with one side of the chain 313. The baffles 318 provide lateral limiting constraints on the chain 313, effectively preventing the chain 313 from running laterally, losing teeth, or deviating during operation. The baffles 318 and the chain 313 have a micro-clearance fit, without pressing or friction, avoiding increased transmission resistance and wear and chipping.

[0050] Specifically, the support 34 serves as the support and positioning base for the petri dish 2. The support 34 includes: a connecting plate 341, one end of which is fixed to the surface of the cylinder 33; two connecting plates 341 are provided on one cylinder 33; a fixing rod 342, one end of which is fixed to the other end of the connecting plate 341; a support plate 343, which is fixed to the end of the fixing rod 342 away from the connecting plate 341; a placement groove is provided on the top of the support plate 343 for limiting the placement of the petri dish 21; a petri dish limiting and unlocking component 35 is installed on the support plate 343 and the cylinder 33, and the outline of the placement groove matches the outer outline of the petri dish 21 to achieve initial limiting and centering positioning when the petri dish 2 is placed.

[0051] The dish body limiting and unlocking component 35 is integrated and installed on the support plate 343 and the cylinder 33. It relies on the support plate 343 to realize assembly and operation, ensuring that the dish body 21 is locked firmly and unlocked smoothly. When manually loading, the dish body 21 is placed stably inside the placement groove of the support plate 343. The groove limit is used to achieve initial centering and positioning, preventing placement deviation and shaking. The driving component 38 acts on the trigger component 37 to activate the dish body limiting and unlocking component 35, which extends from both sides of the support plate 343 to engage the dish body 21, forming a bidirectional limit with the placement groove, completely locking the dish body 21 and ensuring subsequent opening of the lid.

[0052] Example 3, referring to Figures 2 to 10 This is the third embodiment of the present invention, which is based on the first two embodiments.

[0053] Specifically, the trigger 37 is the core of the power transmission and linkage trigger of the entire mechanism, which can realize single-power bidirectional linkage and synchronously control the two major mechanisms of limiting the dish body 21 and lifting the dish lid 22. The trigger 37 includes: a round rod 371, which slides through the round tube 32 and cooperates with the dish lid lifting limit unlocking component 36. The round rod 371 can slide smoothly axially; a conical ring 372, which is rotatably sleeved on one end of the round rod 371 and cooperates with the dish body limit unlocking component 35. The inclined surface of the conical ring 372 is precisely cooperated with the dish body limit unlocking component 35; and a guide bar 373, which slides through the round tube 32 and is fixed to the surface of the round rod 371.

[0054] The guide bar 373 provides anti-rotation guidance, ensuring that the round rod 371 slides only axially and does not deflect circumferentially; the synchronous plate 374 is fixed to one end of the round rod 371 and cooperates with the drive component 38; the steel balls 375, in a number of them, are arranged in a circumferential array and embedded on both sides of one end of the synchronous plate 374, and cooperate with the drive component 38. The multiple sets of steel balls 375 greatly reduce the contact sliding friction resistance, ensuring smooth transmission with the drive component 38 without jamming. The trigger component 37 can convert the horizontal thrust of the drive component 38 into the lateral power for locking the dish body 21 and the vertical power for lifting and lowering the dish cover 22, realizing the sequential linkage of multiple actions from a single power source.

[0055] The driving component 38 advances to contact the synchronous plate 374, pushing the round rod 371 to slide inward along the axial direction of the round tube 32. The round rod 371 drives the conical ring 372 to move and compress the dish body limiting unlocking component 35, thereby locking and fixing the dish body 21. At the same time, the inner end of the round rod 371 pushes the dish cover lifting limiting unlocking component 36, initiating the process of locking and lifting the dish cover 22. When the culture is completed and the dish needs to be removed, the driving component 38 retracts, the round rod 371 resets, the conical ring 372 releases the compression, and each mechanism springs back and unlocks in sequence, completing the reset.

[0056] Specifically, the driving component 38 includes: an electric telescopic rod 381, one end of which is fixed to the inner wall of the housing 11; and a driving frame 382, ​​which is fixed to the other end of the electric telescopic rod 381 and cooperates with the synchronization plate 374 and the steel ball 375 respectively. The driving component 38 is a power source, consisting of the electric telescopic rod 381 and the driving frame 382. The opening of the driving frame 382 faces the synchronization plate 374 and can dock with and cover one end of the synchronization plate 374 and the area of ​​the steel ball 375. The telescopic stroke is precisely limited to avoid over-rushing and squeezing damage to the trigger structure and the culture dish 2 sample.

[0057] After the environment inside the incubator 1 is adjusted to the conditions required for stem cell culture, the electric telescopic rod 381 is extended, driving the drive frame 382 to move, so that the steel ball 375 and the synchronization plate 374 are pushed smoothly, the trigger 37 is fed as a whole, and the locking and opening actions are initiated. After the culture and observation are completed, the electric telescopic rod 381 is retracted, the drive frame 382 is moved back, and all limit and lifting mechanisms automatically spring back to reset under the action of the elastic reset structure, completing the closing and unlocking.

[0058] Specifically, the dish body limiting and unlocking component 35 enables the dish body 21 to automatically lock and elastically unlock. The dish body limiting and unlocking component 35 includes: a locking plate 351, which slides symmetrically through both sides of the support plate 343, and one end of which is adapted to engage with the locking hole 24. The locking plate 351 can be accurately engaged with the locking hole 24 on the dish body 21; one end of the locking plate 351 is provided with a bevel; and a transmission plate 352, which slides through the cylinder 33, and one end of which cooperates with the conical ring 372; both ends of the transmission plate 352 are provided with bevels. One end of the transmission plate 352 engages with the inclined surface of the cone ring 372, and the other end extends into the trigger hole 356 of the clamping plate 351, forming an inclined surface pushing transmission structure. All mating inclined surfaces are polished smoothly to ensure that the pushing and resetting are smooth and without jamming. The round block 353 is fixedly sleeved on the surface of the transmission plate 352. The spring 354 is sleeved on the surface of the transmission plate 352, and its two ends are fixed between the surface of the cylinder 33 and the surface of the round block 353, respectively. The spring 354 pulls the transmission plate 352 to reset under normal conditions.

[0059] Spring 355 is fixed at both ends between the surfaces of the clamping plate 351 and the support plate 343. Spring 355 normally pulls the clamping plate 351 back into the limit position. Trigger hole 356 is opened at one end of the clamping plate 351 and cooperates with one end of the transmission plate 352. The trigger hole 356 is provided with an inclined surface. Guide groove 357 is opened in the support plate 343. Guide block 358 is fixed to the surface of the clamping plate 351 and slides in the guide groove 357. The guide groove 357 and guide block 358 ensure that the clamping plate 351 extends and retracts horizontally in a straight line without deviation and with limited movement. A stop block is fixed to the surface of the transmission plate 352 and abuts against the cylinder 33. The stop block is used to limit the maximum stroke of the transmission plate 352 when it moves back to prevent failure.

[0060] As the trigger 37 moves inward, the cone ring 372 gradually reduces its pressure on the transmission plate 352. Under the rebound action of the spring 354, the transmission plate 352 moves back, and the end slope gradually reduces its pressure on the trigger hole 356. As the spring 355 recovers its deformation, it pushes the two side clamping plates 351 to move, so that one end of the clamping plate 351 is inserted into the clamping hole 24 on the dish body 21, completing the bidirectional locking and fixing of the dish body 21. After the drive 38 retracts, the cone ring 372 presses the transmission plate 352, and the transmission plate 352 overcomes the elastic force of the spring 354 to feed forward. The end slope presses the trigger hole 356, pushing the two side clamping plates 351 to extend outward synchronously. One end of the clamping plate 351 disengages from the clamping hole 24 on the dish body 21, releasing the limitation of the dish body 21 and realizing unlocking.

[0061] Specifically, the lid lifting limit unlocking component 36 realizes the sequential action of "locking the lid first - then lifting - first lowering - then unlocking". The lid lifting limit unlocking component 36 includes: a Z-shaped plate 361, which slides through the cylinder 33; a locking block 362, which slides through one end of the Z-shaped plate 361 and is adapted to engage with the second locking hole 25. The locking block 362 is used to engage the second locking hole 25 on the lid 22; a guide frame 363, which is set in the inner cavity of the cylinder 33 and fixed to the other end of the Z-shaped plate 361; a slider 364, which slides in the guide frame 363; a pulley 365, which rotates on the slider 364; and a drive plate 366, which is rotatably connected to the end of the round rod 371 away from the synchronous plate 374 through a bearing.

[0062] A guide hole 367 is formed on the drive plate 366, and a pulley 365 slides within the guide hole 367. The guide hole 367 is divided into three parts: the two ends are horizontal and the middle part is inclined. The horizontal displacement is converted into vertical lifting displacement through the irregularly shaped guide hole 367, so as to realize the smooth opening and closing of the lid 22. A second spring 368 is sleeved on the surface of the Z-shaped plate 361, and its two ends are fixed to the surface of the cylinder 33 and the surface of the Z-shaped plate 361, respectively. The second spring 368 normally pulls the Z-shaped plate 361 down to keep it in a low position. A roller 369 rotates on the drive plate 366. A track 331 is formed on the cylinder 33, and the roller 369 slides within the track 331. The roller 369 and the track 331 constrain the drive plate 366 to only make horizontal translation, so as to ensure that the transmission trajectory is controllable.

[0063] The lid lifting limit unlocking component 36 also includes: a third spring 3610, which is disposed inside the guide frame 363 and whose two ends are respectively fixed between the inner wall of the guide frame 363 and the surface of the slider 364. The third spring 3610 is used for the elastic reset of the slider 364; a connecting rope 3611, which slides through the Z-shaped plate 361 and whose two ends are respectively fixed between the surface of the locking block 362 and the surface of the slider 364; a second spring piece 3612, whose two ends are fixed between the surface of the Z-shaped plate 361 and the surface of the locking block 362. The second spring piece 3612 normally keeps the locking block 362 in the retracted unlocked state; and a first guide wheel 361. 3. Rotates at one end of the Z-shaped plate 361; guide wheel 2 3614 rotates on the Z-shaped plate 361, and connecting rope 3611 cooperates with guide wheel 1 3613 and guide wheel 2 3614 respectively. Guide wheel 1 3613, guide wheel 2 3614 and connecting rope 3611 realize the sliding of slider 364 to drive the extension and retraction of block 362; limiting groove 3615 is opened in the Z-shaped plate 361; limiting block 3616 is fixed on the surface of block 362 and slides in the limiting groove 3615. Limiting block 3616 and limiting groove 3615 limit the extension and retraction stroke of block 362 to prevent overtravel.

[0064] Trigger 37 pushes drive plate 366 to slide horizontally. At this time, under the action of spring 3610, slider 364 moves within track 331, and pulley 365 does not slide relative to pulley 367 within guide hole 367. Figure 7 The connecting rope 3611 is laid out relative to the wire. Under the rebound action of the second spring piece 3612, the locking block 362 is inserted into the second locking hole 25 to lock the lid 22. The drive plate 366 continues to move horizontally. The pulley 365 first moves in the horizontal section in front of the guide hole 367, then moves into the middle inclined section, and then enters the horizontal section behind the guide hole 367. The guide frame 363, Z-shaped plate 361 and locking block 362 are vertically lifted, which drives the lid 22 to move upward smoothly to open the lid. The reversal is performed when resetting.

[0065] It should be noted that all structural components of this device are made of medical-grade biocompatible materials, which are non-toxic, non-leaching, and non-allergenic. They can withstand high-temperature and high-pressure sterilization, ultraviolet disinfection, and repeated treatment with medical disinfectants. They will not rust or deform with long-term use and are fully compatible with the sterile culture conditions of stem cells.

[0066] During use, the ring-shaped multi-station cyclic conveyor is used: the motor 315 drives the double sprocket 312 and the chain 313 to operate synchronously. Multiple sets of support components 34 on the chain 313 rotate intermittently in a ring, which can carry multiple sets of culture dishes 2 at the same time. When the observation station is reached, the motor 315 automatically locks, and the other stations complete cell culture synchronously. There is no need for frequent manual sample handling, which reduces the frequency of opening and closing of the box 11.

[0067] Automatic locking and opening of the lid in sequence (sealing is completed inside the box): The electric telescopic rod 381 pushes the drive frame 382 to contact the synchronous plate 374, which drives the round rod 371 to slide axially. The conical ring 372 squeezes the limiting unlocking piece 35 of the dish body. The two side plates 351 pop out and lock into the first locking hole 24 on the dish body 21, locking the dish body 21 in both directions to prevent the lid from shifting when it is opened. The round rod 371 drives the drive plate 366 to move horizontally. The irregular guide hole 367 converts the horizontal displacement into a vertical lifting force. First, the locking block 362 locks the second locking hole 25 on the dish lid 22, and then the dish lid 22 is lifted as a whole. The entire process of opening the lid is completed inside the sealed box 11, with no outside air intrusion.

[0068] In-situ transport-free microscopic observation: The lid 22 is inlaid with a silicon nitride light-transmitting window, and the electron beam of the electron microscope 4 can directly penetrate the window for imaging. There is no need to completely open the lid 22 or remove the culture dish 2, which greatly shortens the exposure time of the sample and eliminates cross-contamination during transport from the source. After the culture observation is completed, the drive component 38 retracts, all elastic structures automatically reset, the lid 22 falls back and closes, and the locking plate 351 unlocks and releases the dish body 21.

[0069] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. An infection-preventing protective device for stem cell culture, characterized in that: include, An incubator (1), fixedly installed, is used to provide culture environment conditions for stem cell culture; it includes a box body (11). A culture dish (2) is placed inside the box (11) and is used to hold stem cell culture medium and stem cell samples for culture. The opening and closing protective rotating mechanism (3) is installed inside the box (11), and the culture dish (2) is placed on it to support the placement of the culture dish (2) and realize the opening and closing of the culture dish (2) and the lid. The opening and closing protective rotating mechanism (3) includes: a rotating part (31) installed on the box (11); a round tube (32) symmetrically distributed in a ring array on the rotating part (31); a cylinder (33) rotatably sleeved on one end of the round tube (32); a support part (34) fixed on the surface of the cylinder (33) for supporting the placement of the culture dish (2); and a dish limiting and unlocking part (35). Installed on the support (34) and the cylinder (33), it is used for limiting and unlocking the petri dish (2); the lid lifting limit unlocking component (36) is installed on the support (34) and the cylinder (33) for limiting and unlocking the petri dish (2) lid, and controlling the opening and closing between the lid and the dish body; the trigger component (37) is installed on the round tube (32) and the lid lifting limit unlocking component (36), and cooperates with the dish body limiting unlocking component (35) and the lid lifting limit unlocking component (36); the drive component (38) is installed on the box (11) and cooperates with the trigger component (37); An electron microscope (4) is mounted through the box (11) for in-situ observation and detection of stem cell samples cultured inside the box (11).

2. The infection-preventing stem cell culture protective device as described in claim 1, characterized in that: The culture dish (2) includes: a dish body (21) placed on a support (34); a dish lid (22) covering the dish body (21); a silicon nitride window (23) embedded in the dish lid (22); a first locking hole (24) on the dish body (21) and adapted to engage with the dish body limiting unlocking component (35); and a second locking hole (25) on the dish lid (22) and adapted to engage with the dish lid lifting limiting unlocking component (36).

3. The infection-resistant stem cell culture protective device as described in claim 1 or 2, characterized in that: The rotating component (31) includes: a rotating rod (311) that rotates up and down inside the housing (11); a sprocket (312) that is fixedly sleeved on both ends of the two rotating rods (311); a chain (313) that drives the surface of the sprocket (312) on the two rotating rods (311); a connecting plate (314) that is fixed on the chain (313), and a round tube (32) that passes through and is fixed to the end of the connecting plate (314) away from the chain (313); and a motor (315) that is installed on one side of the housing (11), and the output shaft is fixed to one end of the rotating rod (311).

4. The infection-preventing stem cell culture protective device as described in claim 3, characterized in that: The rotating component (31) further includes: a reinforcing rod (316), both ends of which are fixed to two horizontally adjacent connecting plates (314); a short rod (317), which is fixed to the inner wall of the box (11); and a baffle (318), which is fixed to one end of the short rod (317) and cooperates with one side of the chain (313).

5. The infection-preventing stem cell culture protective device as described in claim 3, characterized in that: The support member (34) includes: a connecting plate (341), one end of which is fixed to the surface of the cylinder (33); a fixing rod (342), one end of which is fixed to the other end of the connecting plate (341); a support plate (343), which is fixed to the end of the fixing rod (342) away from the connecting plate (341); a placement groove is provided on the top of the support plate (343), and the vessel limiting unlocking member (35) is installed on the support plate (343) and the cylinder (33).

6. The infection-preventing stem cell culture protective device as described in any one of claims 1, 2, 4, or 5, characterized in that: The trigger (37) includes: a round rod (371) that slides through the round tube (32) and cooperates with the lid lifting limit unlocking component (36); a conical ring (372) that is rotatably sleeved on one end of the round rod (371) and cooperates with the lid limiting unlocking component (35); a guide strip (373) that slides through the round tube (32) and is fixed to the surface of the round rod (371); a synchronization plate (374) that is fixed to one end of the round rod (371) and cooperates with the drive component (38); and steel balls (375) that are rotatably embedded in a circular array on both sides of one end of the synchronization plate (374) and cooperate with the drive component (38).

7. The infection-resistant stem cell culture protective device as described in claim 6, characterized in that: The drive unit (38) includes: an electric telescopic rod (381), one end of which is fixed to the inner wall of the housing (11); and a drive frame (382), which is fixed to the other end of the electric telescopic rod (381) and cooperates with the synchronization plate (374) and the steel ball (375) respectively.

8. The infection-resistant stem cell culture protective device as described in claim 6, characterized in that: The limiting and unlocking component (35) of the vessel body includes: a locking plate (351), which slides through both sides of the support plate (343) and one end is adapted to engage with the locking hole (24); a transmission plate (352), which slides through the cylinder (33) and one end is engaged with the conical ring (372); a round block (353), which is fixedly sleeved on the surface of the transmission plate (352); a spring (354), which is sleeved on the surface of the transmission plate (352) and both ends are fixed between the surface of the cylinder (33) and the surface of the round block (353); a spring piece (355), whose two ends are fixed between the surface of the locking plate (351) and the surface of the support plate (343); and a trigger hole (356), which is opened at one end of the locking plate (351) and engages with one end of the transmission plate (352).

9. The infection-resistant stem cell culture protective device as described in claim 7 or 8, characterized in that: The lid lifting limit unlocking component (36) includes: a Z-shaped plate (361) that slides through the cylinder (33); a locking block (362) that slides through one end of the Z-shaped plate (361) and is fitted with the locking hole (25); a guide frame (363) that is located in the inner cavity of the cylinder (33) and fixed to the other end of the Z-shaped plate (361); a slider (364) that slides inside the guide frame (363); a pulley (365) that rotates on the slider (364); and a drive plate (366) that connects to the round rod via a bearing. 371) The end away from the synchronous plate (374) is rotatably connected; the guide hole (367) is opened on the drive plate (366), and the pulley (365) slides in the guide hole (367); the second spring (368) is sleeved on the surface of the Z-shaped plate (361), and its two ends are respectively fixed to the surface of the cylinder (33) and the surface of the Z-shaped plate (361); the roller (369) rotates on the drive plate (366); the cylinder (33) is provided with a track (331), and the roller (369) slides in the track (331).

10. The infection-resistant stem cell culture protective device as described in claim 9, characterized in that: The lid lifting limit unlocking component (36) further includes: spring three (3610), which is set inside the guide frame (363) and its two ends are respectively fixed between the inner wall of the guide frame (363) and the surface of the slider (364); connecting rope (3611), which slides through the Z-shaped plate (361) and its two ends are respectively fixed between the surface of the block (362) and the surface of the slider (364); spring piece two (3612), whose two ends are fixed between the surface of the Z-shaped plate (361) and the surface of the block (362); guide wheel one (3613), which rotates at one end of the Z-shaped plate (361); guide wheel two (3614), which rotates on the Z-shaped plate (361), and the connecting rope (3611) cooperates with guide wheel one (3613) and guide wheel two (3614) respectively.

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