A high-yield breeding liquid chip for 40k of monopterus albus and a preparation method and application thereof

CN122879401APending Publication Date: 2026-10-09SHANGHAI ACAD OF AGRI SCI
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Patent Information

Application Number
CN202611210365.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-08-11
Publication Date
2026-10-09

AI Technical Summary

Benefits of technology

本发明首次开发了基于靶向捕获测序的黄鳝40K高产育种液相芯片(“鳝芯1号”)。芯片的位点包括背景SNP位点以及与黄鳝重要性状关联性高的前景SNP位点(功能位点)。该液相芯片位点的测序质量高,基因分型准确率高,克服了黄鳝在测序质量低时基因分型效果差的问题。该液相芯片以严格的标准筛选可用于开发芯片的SNP位点,在设计探针时将GC含量控制在30%~70%之间,同源区域数控制在5个以内,大幅度提高了位点所在序列的杂交稳定性,以及芯片捕获DNA片段的捕获率。

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Abstract

The application discloses a rice field eel SNP site combination and a 40K high-yield breeding liquid phase chip prepared based on the SNP site combination and application thereof. The breeding liquid phase chip comprises probes for detecting 44832 SNP sites; position information of the 44832 SNP sites is determined based on sequence alignment of a reference version of a rice field eel genome, and the reference version of the rice field eel genome is GWHBEHV00000000. The gene chip can be applied to genotyping or genome selection breeding of the rice field eel. The breeding liquid phase chip can effectively reduce the cost of genotyping and genome selection breeding of the rice field eel, improve breeding accuracy and efficiency, and promote the green and healthy development of the rice field eel industry.
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Description

Technical Field

[0001] This invention belongs to the field of gene chips, specifically relating to the 40K high-yield breeding liquid phase chip of the yellow eel, its preparation method and application. Background Technology

[0002] Yellow eel ( Monopterus albus The yellow eel (Solanum lycopersicum) is an important and high-quality freshwater aquaculture species in my country, with an annual output value of approximately 100 billion yuan, making it a significant economic growth point for the aquaculture industry. Its growth rate, disease resistance, and reproductive performance directly determine the economic benefits of aquaculture. However, for a long time, the creation, preservation, breeding, and application of high-quality germplasm resources for yellow eels have been lacking internationally. A weak seed industry foundation has become a major constraint on the industry's development, and traditional breeding models have significant shortcomings. Molecular markers are the core technology of molecular breeding, identifying genetic polymorphisms in individuals by marking variations in nucleotide sequences. With the development of molecular marker technology, molecular markers such as restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and simple sequence repeats (SSR) have been proposed and used, promoting the development of the field of molecular genetic breeding. However, these technologies are complex to operate and have long sequencing cycles, making it difficult to support the needs of large-scale molecular genetic breeding. Single nucleotide polymorphism (SNP) is a new generation of molecular marker technology that can mark variations in a single nucleotide in the genome. SNPs are widely distributed, have high genome coverage, high throughput, high accuracy, and are easy to analyze, playing a crucial role in genetic breeding technologies such as population genetic structure analysis and germplasm identification. Common methods for obtaining SNPs include whole-genome resequencing and simplified genome sequencing. These sequencing technologies can identify SNPs across the entire genome and discover loci that can be used for genetic breeding. However, while these sequencing technologies identify high locus abundance, they are also costly and have relatively low analysis efficiency. Therefore, SNP microarrays have gained widespread attention and use due to their high analysis efficiency, high depth, and low cost. SNP liquid-phase microarrays based on whole-genome targeted capture sequencing (GBTS) achieve high-depth sequencing of specific loci by reducing library abundance. This microarray designs probes that are complementary to the bases of the target sequence at the target locus and captures the probes to obtain specific fragments for sequencing, thus enabling locus detection within those fragments. Liquid-phase microarrays significantly reduce sequencing costs, increase sequencing depth and efficiency at specific loci, have no requirements for locus abundance, and allow for the continuous addition or deletion of loci after development. These advantages have also enabled GBTS-based liquid phase chips to play an important role in the breeding and genetic analysis of a wide range of plants and animals.

[0003] Currently, GBTS-based SNP liquid-phase chips have been developed and applied in a large number of fish and some marine organisms, but there are no liquid-phase chips specifically developed for the breeding of swamp eels. Therefore, it is necessary to research and develop liquid-phase chips for the breeding of swamp eels. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention first provides a combination of SNP sites in the swamp eel. This combination consists of 44,832 SNP sites, the location information of which is determined by sequence alignment based on a reference version of the swamp eel genome, namely GWHBEHV00000000. Specifically, 44,832 SNP loci are distributed on the 12 chromosomes of the yellow eel, including 5,103 on chromosome 1, 5,073 on chromosome 2, 4,221 on chromosome 3, 4,085 on chromosome 4, 4,268 on chromosome 5, 3,890 on chromosome 6, 3,341 on chromosome 7, 3,681 on chromosome 8, 3,297 on chromosome 9, 2,733 on chromosome 10, 2,261 on chromosome 11, and 2,879 on chromosome 12. Information on 44,832 SNP sites is shown in Tables 1 to 12, which are the SNP site information tables on chromosomes CHR01 to CHR12, respectively.

[0005] The present invention also provides a liquid phase chip for high-yield breeding of swamp eels at 40K, which includes probes for detecting the above-mentioned 44,832 SNP sites; the location information of the 44,832 SNP sites is determined by sequence alignment based on a reference version of the swamp eel genome, the reference version of the swamp eel genome being GWHBEHV00000000. Specifically, the 44,832 SNP loci include 44,793 background SNP loci and 39 foreground SNP loci (functional loci). Background SNPs were obtained by screening high-depth resequencing data from 376 samples of parents from 21 geographical populations and core breeding groups; prospective SNPs were located through genome-wide association analysis (GWAS). Specifically, each SNP site corresponds to two single-stranded nucleotide probes for specific detection of that site, which are used to target and capture that site in swarm genotyping or genome-wide association analysis.

[0006] The second objective of this invention is to provide a method for preparing the above-mentioned 40K high-yield breeding liquid phase chip for swamp eels, which includes the following steps: S1. Extract DNA from the swamp eel and resequencing it; S2. Align the resequencing results with the reference version of the swamp eel genome and perform genotyping; S3. Screen to obtain a background SNP site set, and select background SNP sites that are evenly distributed on the chromosome from the background SNP site set. S4. Based on the association with important traits of the swamp eel, prospective SNP sites (functional sites) were screened from the SNP sites. S5. Design probes for detecting SNP sites targeting both background and foreground SNP sites; Furthermore, in step S3, the screening criteria for background SNP sites are: biallelic SNPs; MAF ≥ 0.05; deletion rate ≤ 10; and heterozygosity rate ≤ 50%. Specifically, from 21,009,900 background sites, 44,793 background sites are retained after screening according to the above principles. Furthermore, in step S4: genome-wide association analysis was performed using GWAS software to screen 39 SNP sites that were highly associated with the high-yield trait of yellow eel; Furthermore, in step S5, probe synthesis is based on the following principles: probe length 110 bp; probe GC content 30-70%; maximum number of similar fragments ≤ 5 (i.e., the maximum upper limit of the number of specific similar fragments of the probe on the reference genome). A third objective of this invention is to provide the application of the aforementioned 40K high-yield breeding liquid phase chip for swamp eel genotyping. The liquid phase chip exhibits high individual detection rate and high site polymorphism, indicating good quality and enabling high-quality and efficient genotyping.

[0007] Specifically, the above-mentioned 40K high-yield breeding liquid phase chip for swamp eel genotyping was applied using the following steps: S1. Extract DNA from the swamp eel and construct a high-throughput sequencing library; S2. DNA fragments containing target SNP sites in a library were captured using a 40K high-yield breeding liquid phase chip for the swamp eel, and then amplified and purified. S3. Perform high-throughput sequencing and align the results to the reference genome of the swamp eel (reference version GWHBEHV00000000) to obtain the genotype of the swamp eel to be tested at the target SNP site.

[0008] The fourth objective of this invention is to provide the application of the above-mentioned 40K high-yield breeding liquid phase chip for swamp eel genome selection. The SNP site genome selection prediction accuracy using this liquid phase chip is high, proving that this breeding liquid phase chip can be used in swamp eel genome selection.

[0009] The fifth objective of this invention is to provide the application of the above-mentioned SNP site combinations of swamp eels in swamp eel genotyping and in swamp eel genome selection.

[0010] Compared with the prior art, the present invention has the following beneficial effects: This invention is the first to develop a liquid-phase microarray ("Shanxin No. 1") for high-yield breeding of swamp eels based on targeted capture sequencing. The microarray includes background SNP sites and prospective SNP sites (functional sites) highly associated with important traits in swamp eels. This liquid-phase microarray exhibits high sequencing quality and high genotyping accuracy, overcoming the problem of poor genotyping results when sequencing quality is low. The liquid-phase microarray uses strict criteria to screen SNP sites suitable for microarray development. During probe design, the GC content is controlled between 30% and 70%, and the number of homologous regions is limited to no more than 5, significantly improving the hybridization stability of the sequences containing the sites and the capture rate of DNA fragments captured by the microarray.

[0011] The development of this liquid-phase chip is based on targeted capture technology, allowing for the addition of new loci as needed, offering high flexibility. Simultaneously, this chip will significantly reduce the cost of genotyping and breeding of swamp eels, improving the accuracy and efficiency of genotyping analysis. Furthermore, the loci within the chip demonstrate excellent performance in predicting genomic selection for high-yield traits in swamp eels, exhibiting high and stable accuracy. These loci can be used for screening high-quality individuals and constructing superior populations, accelerating the breeding of new swamp eel varieties and further improving breeding accuracy. Attached Figure Description

[0012] Figure 1 This is a distribution diagram of SNP sites in each chromosome of the liquid phase chip for high-yield breeding of swamp eel using the 40K method in Example 1; Figure 2 This is a genotyping MAF statistical chart of the yellow eel in Example 2; Figure 3 This is a statistical chart showing the accuracy of genome selection for body length and weight of the eel in Example 3. Detailed Implementation

[0013] The embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of the invention.

[0014] Example 1 The preparation of a 40K high-yield breeding liquid phase chip for swamp eels includes the screening of swamp eel SNP locus combinations and the preparation of the 40K high-yield breeding liquid phase chip for swamp eels. The specific steps are as follows: S1. DNA was extracted from swamp eel samples from 21 geographical populations in China and abroad (including Ankang AK, Chengdu CD, Chongqing CQ, Changsha CS, Dali DL, Dongying DY, Indonesia ID, Jinan JN, Lu'an LA, Luzhou LZ, Myanmar MYA, Puyang PH, Shanghai SH, Suzhou SZ, Tai'an TA, Vietnam VN, Xiantao XT, Xinyang XY, Yichun YC, Yueyang YY, and Zhanjiang ZJ). The swamp eels were resequencing using a high-throughput sequencing platform, with an average sequencing depth of 20X.

[0015] S2. Use Fastp to filter the obtained raw reads into high-quality clean reads. Then use BWA-mem and Samtools to align the clean reads to the swamp eel reference genome GWHBEHV00000000 and use GATK for genotyping.

[0016] S3. The screening criteria for background SNP sites are: biallelic SNPs; MAF ≥ 0.05; deletion rate ≤ 10; and heterozygosity rate ≤ 50%. Specifically, from 21,009,900 background sites, 44,793 background sites were retained after screening according to the above principles.

[0017] S4. Genome-wide association analysis was performed using GWAS software to identify 39 SNP loci (functional loci) that were highly associated with important traits of the yellow eel.

[0018] After merging functional and background sites, a total of 44,832 high-quality SNP sites were obtained.

[0019] S5. Probes were designed for background SNP sites and foreground SNP sites (merged into 44,832 high-quality SNP sites). Probes were designed based on GC content between 30% and 70% and the number of homologous regions ≤ 5, with a probe length of 110 bp.

[0020] Information on 44,832 SNP loci is shown in Tables 1 to 12. Tables 1 to 12 are the SNP loci information tables on chromosomes CHR01 to CHR12, respectively.

[0021] Table 1. CHR01 site information (sites in CHR01 / reference bases / mutant bases) Location, ref, alt, Location, ref, alt, Location, ref, alt, Location, ref, alt, Location, ref, alt, position, ref, alt 25879679, T, C, 7609596, T, C, 23496955, C, A, 37770321, A, G, 54938744, G, A, 70963934, C, T 26709671, C, A, 7625570, A, T, 23507418, G, T, 37775836, A, G,54941052, A, G, 70972373, A, G 54425570, A, G, 7629868, G, C, 23530252, A, G, 37813408, G, T, 54953403, A, T, 71036035, C, T 85525721, A, T, 7634828, C, G, 23564371, G, A, 37838111, G, C,54956642, T, C, 71062735, G, A 21471797, G, A, 7643715, G, A, 23719045, G, C, 37937605, G, A,54965863, A, G, 71077074, A, G 21471824, G, A, 7676863, T, C, 23724566, A, G, 37941133, A, G,54973953, A, C, 71084683, A, C 21472037, T, C, 7693220, T, C, 23732375, T, C, 37952976, A, C,54981327, C, T, 71202814, T, C 21472427, A, G, 7699892, T, C, 23739526, T, C, 37960710, A, T,54984591, T, C, 71228807, G, A 21472451, G, A, 7704636, A, G, 23757864, T, C, 37962006, G, A,54995114, G, C, 71247577, G, A 21472509, G, T, 7712658, A, G, 23763020, G, A, 37969144, T, A,55001190, A, G, 71287336, T, C 21472553, C, T, 7722917, T, C, 23782332, C, T, 38022841, G, T,55020127, T, C, 71292517, T, A 21472568, A, C, 7730018, C, T, 23793714, C, T, 38024425, T, C,55030032, A, G, 71299726, T, A 21472586, C, T, 7746268, T, C, 23823631, G, A, 38036862, G, C,55045078, A, C, 71305274, T, G 21472603, C, T, 7758999, C, T, 23849617, A, T, 38043750, T, C,55050159, G, C, 71325352, G, C 21472649, A, G, 7764467, T, C, 23854291, A, G, 38047908, T, G,55060420, G, T, 71417228, A, T 21472710, G, A, 7778705, A, T, 23864243, C, T, 38070221, T, G,55070988, A, T, 71424197, G, A 21472736, C, T, 7782575, C, T, 23873717, C, T, 38093936, G, C,55107023, T, C, 71430030, A, G 21472790, C, T, 7791512, G, A, 23907530, A, T, 38096886, G, A,55152611, T, C, 71437045, C, A 21473168, T, C, 7828340, C, T, 23915966, A, G, 38105111, A, G,55161038, A, G, 71452454, A, T 21473182, G, A, 7857235, G, A, 23917975, A, T, 38114434, C, T,55169240, C, T, 71474861, A, T 21473197, A, T, 7875956, G, A, 23935005, G, T, 38119861, A, G,55178371, T, C, 71479396, C, T 21473227, T, C, 7880453, A, G, 23956495, G, A, 38134088, G, T,55183800, A, G, 71490955, A, T 21473261, C, G, 7889310, A, G, 23967891, G, A, 38150143, T, A,55188227, C, T, 71492975, T, C 21473303, C, T, 7905070, T, A, 23979421, G, A, 38153386, G, A,55195852, A, T, 71503488, A, G 21473351, C, T, 7922848, T, A, 23997003, G, A, 38160549, T, C,55203721, C, T, 71508133, T, C 21473440, T, C, 7929581, A, C, 24041811, A, G, 38168645, T, A,55216116, A, G, 71520356, C, T 21473590, G, A, 7935729, C, G, 24069594, A, T, 38172957, G, A,55233963, G, C, 71527229, A, G 21473614, C, T, 7945259, G, T, 24071816, G, A, 38180726, C, A,55238199, G, C, 71539903, T, G 21473641, T, A, 7956838, A, G, 24084284, C, A, 38194663, C, T,55248034, A, G, 71543891, A, G 21473656, G, A, 7969009, C, T, 24085956, A, G, 38220781, A, T,55253114, C, T, 71551407, T, C 21473731, G, A, 7980931, C, G, 24097131, G, A, 38231609, G, A,55262272, G, A, 71580966, T, G 21473832, T, C, 7989953, C, A, 24103452, G, A, 38266321, T, C,55275398, A, T, 71601795, T, C 31976565, C, T, 8004680, C, A, 24107005, G, A, 38271541, C, T,55279586, A, C, 71610737, A, G 39568601, C, T, 8012005, G, A, 24131028, A, G, 38281238, C, T,55285152, G, C, 71622141, T, C 39568615, C, T, 8021407, G, A, 24137716, G, A, 38287797, C, G,55296704, A, G, 71647090, C, A 39569536, G, A, 8030512, A, G, 24218885, G, A, 38295415, C, T,55300816, A, G, 71653996, C, T 39569695, C, G, 8041552, A, G, 24232113, G, A, 38303335, G, A,55316857, C, T, 71667927, G, A 39569725, T, C, 8075322, C, T, 24249633, C, T, 38309064, T, C,55320711, T, C, 71674092, A, G 4668708, G, T, 8083455, C, T, 24254019, C, G, 38325894, T, G,55332225, A, G, 71756527, A, G 4668730, A, G, 8140478, G, T, 24273948, A, G, 38328871, A, G,55334553, A, T, 71760375, A, G 4668803, T, G, 8150890, C, T, 24303504, G, A, 38338651, A, T,55350764, A, C, 71792061, A, T 55553864, G, A, 8193884, C, G, 24342563, A, G, 38348156, G, A,55359347, C, T, 71793110, C, G 55556621, A, G, 8196538, C, T, 24353263, G, T, 38355112, C, T,55371982, C, T, 71837870, T, A 67572948, G, A, 8204083, T, C, 24359732, G, A, 38368112, T, A,55376205, A, G, 71873451, A, G 74861022, G, C, 8213814, A, G, 24379130, G, A, 38374994, A, T,55425788, G, C, 71885472, A, G 74861058, A, C, 8223956, C, A, 24389321, A, T, 38377929, T, C,55442013, G, A, 71895180, T, C 74861100, A, C, 8244199, G, T, 24409781, A, T, 38388543, A, C,55446173, C, T, 71904808, A, T 74861115, C, A, 8261333, T, C, 24419109, C, T, 38389627, T, C,55454616, A, C, 71908793, A, G 74862534, C, T, 8265257, G, C, 24435157, G, A, 38396380, A, G,55461451, A, G, 71975440, G, C 74862551, C, T, 8278913, A, C, 24450025, C, A, 38414011, C, A,55471546, C, T, 71977532, A, G 74862568, C, A, 8284968, G, A, 24462711, T, C, 38431181, T, G,55474050, G, A, 71991986, G, A 81251357, G, A, 8296929, T, C, 24473808, C, T, 38434848, G, T,55505545, G, A, 72021310, A, G 82323049, C, T, 8319189, G, A, 24483372, C, A, 38444902, C, A,55627372, C, A, 72045288, G, T 82323060, A, T, 8323492, T, C, 24491137, G, A, 38452266, T, G,55649075, C, T, 72047280, A, C 82323070, A, G, 8339685, G, A, 24576485, C, A, 38461850, C, T,55767641, T, C, 72064102, G, A 82323088, A, C, 8348789, A, G, 24580711, T, C, 38468122, T, C,55793571, T, C, 72072719, A, C 82323121, A, G, 8349911, G, A, 24585046, C, T, 38479267, A, G,55801791, G, T, 72075415, T, C 82354537, C, A, 8368127, A, C, 24605177, T, C, 38481725, T, G,55813615, G, A, 72093466, G, A 82593248, C, T, 8372871, G, C, 24613676, G, T, 38489792, T, C,55892470, T, G, 72098999, G, T 82967585, G, A, 8380651, G, A, 24701668, G, A, 38496409, T, C,55915781, T, A, 72102176, G, T 82967634, G, T, 8395201, G, C, 24707015, C, T, 38519912, A, G,55983194, G, T, 72135050, G, A 10006190, A, G, 8419182, G, C, 24710735, C, T, 38528569, T, C,56258010, A, G, 72156901, A, T 10051404, A, T, 8424150, T, C, 24719639, G, T, 38534320, C, A,56268521, C, T, 72162746, C, G 10057054, A, T, 8426755, A, G, 24725055, C, T, 38537092, C, G,56279908, A, G, 72165950, G, A 10065475, C, T, 8480855, T, C, 24748388, G, T, 38545731, C, T,56286729, C, T, 72172796, T, G 10071428, G, A, 8485384, G, A, 24755303, A, T, 38556054, G, A,56296114, T, C, 72186279, A, T 1007990, C, A, 8491265, G, T, 24775682, C, A, 38562708, T, A,56304704, A, G, 72248357, C, G 10082376, T, C, 8502370, C, T, 24784632, A, G, 38572054, C, G,56310648, A, G, 72255675, A, G 10097959, T, A, 8505180, T, C, 24793597, G, A, 38585321, G, A,56319155, A, T, 72262119, A, T 10106734, T, C, 8514929, C, A, 24801803, A, G, 38592278, C, T,56327323, G, A, 72275835, A, G 10123294, G, C, 8522404, A, G, 24819768, T, C, 38609493, T, A,56336181, A, G, 72277568, A, G 10127834, T, G, 8528090, C, A, 24847399, A, T, 38619081, A, G,56339391, C, G, 72291271, G, A 1013413, C, G, 8538814, T, C, 24854771, T, A, 38643905, C, T,56355168, G, A, 72295778, A, C 10134248, A, C, 8555871, A, G, 24862094, C, T, 38659346, A, G,56362125, T, C, 72300757, C, T 10140400, C, A, 8563322, T, C, 24865362, T, C, 38679424, T, A,56368194, C, T, 72314339, G, A 10150277, T, C, 8568422, T, C, 24876355, T, C, 38695825, C, T,56382569, A, G, 72345245, T, A 10157369, T, C, 8583043, A, T, 24891706, C, T, 38711283, G, A,56399825, A, G, 72351020, T, A 10163815, T, C, 8588330, A, G, 24894752, T, C, 38734163, T, C,56411841, G, A, 72362292, G, A 10170738, A, T, 8643189, T, A, 24900324, C, A, 38742251, T, C,56419184, G, A, 72388797, C, T 10178516, A, C, 8648680, C, T, 24911154, G, T, 38749404, G, A,56424432, G, A, 72409941, G, A 10200226, T, G, 8655245, T, C, 24917858, G, A, 38754989, G, C,56429420, T, A, 72415736, C, T 10206214, A, C, 8662641, C, T, 24948006, C, T, 38767799, A, G,56437431, C, T, 72424297, T, G 1021092, G, A, 8665240, C, T, 24958828, A, G, 38778306, T, C,56443494, T, C, 72438623, T, C 10233848, T, G, 8676200, T, G, 24971840, A, G, 38787981, G, T,56450366, T, A, 72442196, T, C 10292288, T, C, 8691626, A, G, 24982059, C, T, 38791425, A, T,56458139, G, A, 72470819, A, C 1029672, A, G, 8695399, C, G, 24985300, T, C, 38804129, C, G,56464563, C, T, 72481541, G, A 10332173, G, C, 8701415, G, A, 24995934, T, G, 38812688, A, G,56472604, G, T, 72488515, C, T 10338239, T, C, 8710994, A, T, 25000741, G, A, 38835649, G, A,56481630, G, A, 72519221, T, C 10349432, A, G, 8721073, A, C, 25007427, G, T, 38856063, C, T,56503571, C, T, 72537437, A, C 10354289, T, G, 8734998, C, T, 25016237, A, T, 38859924, G, A,56517064, C, A, 72563617, T, G 10359351, G, T, 8736169, G, A, 25025846, T, G, 38894092, T, G,56784885, C, T, 72570319, C, T 10371218, C, T, 8743605, T, C, 25036350, G, C, 38896355, G, A,56791853, T, G, 72650097, G, A 10377842, C, A, 8776324, A, G, 25043320, C, T, 38910607, G, C,56796491, T, C, 72662442, G, T 1038514, G, T, 8787716, G, A, 25052409, G, A, 38922770, G, A,56802166, C, T, 72664174, T, C 10385097, C, T, 8801701, G, T, 25059869, A, G, 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54185518, T, C,69000592, T, C, 85268740, T, A 6763534, G, A, 22762106, C, T, 36342645, A, G, 54191740, T, G,69012081, A, G, 85288714, C, T 6769436, T, C, 22785928, T, A, 36346558, C, T, 54203148, C, T,69116221, T, C, 85294893, T, C 6786528, A, G, 22790267, G, A, 36361530, C, T, 54223494, G, A,69117735, G, A, 85306675, A, T 6796345, A, G, 22805513, A, T, 36367704, T, A, 54231337, A, G,69131371, T, C, 85318225, A, C 6798675, G, A, 22809084, C, T, 36384933, T, C, 54239444, A, G,69135962, C, T, 85326844, T, C 6812376, C, T, 22819308, C, T, 36647544, T, G, 54260871, A, G,69141027, G, A, 85332638, C, G 6823643, G, A, 22830841, T, C, 36880808, A, C, 54277010, C, T,69154426, T, C, 85429527, G, T 6837849, T, C, 22835866, C, T, 36894575, G, A, 54283180, C, T,69184812, T, G, 85442596, T, G 6845460, C, T, 22846975, C, T, 36916514, T, C, 54288377, G, A,69192192, A, G, 85458353, C, T 6853360, C, G, 22851890, A, T, 36922162, A, G, 54300844, A, T,69201266, A, G, 85470901, C, T 6863546, T, C, 22963723, C, T, 36934093, A, C, 54324067, C, T,69208625, G, A, 85484170, A, G 6918685, C, T, 22969350, A, C, 36940768, A, C, 54345196, A, G,69210635, A, G, 85487348, G, A 6941873, C, G, 22980233, C, T, 36944729, C, T, 54363061, T, A,69243851, C, T, 85496179, A, G 6945252, G, A, 22986554, T, C, 36952208, T, G, 54367413, A, G,69248170, T, A, 85500900, T, C 6985766, T, A, 23001834, T, A, 36965380, T, C, 54377955, A, G,69291604, T, A, 85526801, T, C 7012720, G, A, 23016096, G, A, 36973546, T, C, 54394128, C, T,69298525, A, G, 85529154, G, T 7020751, G, A, 23023199, A, G, 36985365, C, T, 54408739, C, T,69301654, A, T, 85537433, A, C 7032491, G, A, 23031648, C, T, 36998167, T, C, 54418381, G, T,69331627, C, T, 85548509, G, T 7041622, A, T, 23038189, T, A, 37016339, A, G, 54425297, C, T,69351063, G, A, 85554986, C, G 7052528, A, G, 23039768, A, C, 37030686, T, C, 54433144, G, T,69356489, G, C, 85559271, T, C 7062130, A, C, 23050952, C, T, 37034922, T, C, 54440999, T, C,69370328, T, C, 85564951, T, G 7078166, G, T, 23055450, G, A, 37045414, A, G, 54442803, G, T,69397872, A, G, 85684396, C, T 7082079, G, T, 23066714, T, C, 37053557, T, C, 54449358, C, T,69417366, A, G, 85696129, G, A 7086048, C, T, 23088313, T, C, 37055205, C, T, 54462206, A, G,69430418, A, G, 85702207, A, G 7097219, A, C, 23091865, C, T, 37066568, C, T, 54468570, A, G,69435959, A, T, 85721010, T, A 7105924, C, T, 23098946, G, A, 37079481, A, C, 54472291, T, C,69443338, A, T, 85728062, C, T 7110480, T, G, 23121276, G, A, 37090198, G, C, 54480142, T, G,69461508, C, T, 85735831, C, T 7126741, A, G, 23124631, C, T, 37094110, A, C, 54487376, C, T,69462708, G, T, 85741382, G, C 7128481, G, A, 23141271, C, T, 37100275, G, A, 54523505, T, C,69473872, A, T, 85873214, A, G 7154983, T, A, 23148973, T, C, 37116747, C, G, 54552009, T, A,69477624, C, T, 85887706, G, A 7170298, A, G, 23153475, T, C, 37119676, C, T, 54558960, C, T,69501706, C, T, 85941384, C, T 7182317, G, A, 23162803, A, T, 37204825, T, A, 54564642, C, T,69509074, A, G, 85943083, C, T 7188114, G, T, 23177889, A, G, 37214030, T, G, 54571011, T, G,69554478, A, G, 85957771, T, C 7193099, C, T, 23188878, C, T, 37223314, A, G, 54576472, A, T,69580311, C, T, 85980871, G, A 7197688, A, G, 23196184, A, C, 37230274, C, T, 54594375, T, G,69701122, A, G, 86082759, G, A 7302725, C, T, 23206319, G, A, 37254881, A, G, 54599406, G, A,69708003, G, A, 86210928, G, A 7323436, A, C, 23208912, C, T, 37260436, C, T, 54606227, T, G,69720941, G, A, 86441540, G, A 7329631, A, G, 23241463, G, A, 37270712, G, A, 54618261, G, A,69726383, T, C, 86541043, T, C 7332173, C, T, 23244755, A, G, 37276470, A, C, 54635062, G, A,69732002, A, G, 86573905, T, A 7339921, A, C, 23256408, C, T, 37322771, G, T, 54640202, G, A,70103893, A, C, 86638956, A, G 7349721, T, C, 23281235, G, A, 37393714, C, T, 54650925, T, A,70109193, G, C, 86647363, T, A 7370542, C, T, 23304041, T, G, 37426925, G, T, 54714359, A, G,70116755, T, C, 86703105, A, G 7387274, G, A, 23319427, C, T, 37441065, A, T, 54715927, A, G,70123860, A, G, 86731517, T, C 7404106, T, C, 23325206, A, G, 37500262, T, C, 54726378, G, A,70187131, A, C, 86758097, T, C 7419483, A, C, 23335017, C, T, 37527178, T, A, 54730391, C, T,70234907, A, G, 86776730, A, G 7423986, A, G, 23345749, T, A, 37644220, A, G, 54742767, A, C,70247694, T, C, 86788448, G, T 7441317, A, T, 23353466, A, G, 37648443, C, G, 54748454, A, G,70273892, G, A, 86794282, C, T 7456795, C, T, 23368697, T, C, 37653057, A, G, 54757088, T, C,70279585, G, A, 86847306, G, A 7460801, T, A, 23375069, G, C, 37662656, T, G, 54775225, A, G,70301871, C, T, 86859551, C, T 7477171, C, T, 23376523, C, T, 37686358, G, A, 54782901, G, T,70335100, T, C, 86877798, C, G 7478751, C, T, 23385419, T, A, 37693868, T, G, 54786236, T, C,70407929, A, G, 86887251, C, T 7503132, A, T, 23394488, C, T, 37697780, A, G, 54819035, G, C,70488976, T, G, 86899965, C, T 7543798, T, C, 23398883, G, A, 37702921, C, G, 54821959, G, A,70500791, A, T, 86930098, G, A 7553785, C, T, 23417158, G, A, 37728894, A, G, 54845646, G, A,70503266, A, T, 86944193, C, T 7559853, G, T, 23436457, C, T, 37734162, T, C, 54869898, C, T,70729311, T, C, 86946524, G, A 7564934, C, T, 23441778, G, A, 37742963, A, G, 54893204, T, C,70838986, T, A, 86959010, A, G 7572294, T, A, 23449099, G, A, 37744244, T, C, 54900685, C, T,70906346, G, A, 86989713, T, A 7582536, A, G, 23475451, G, A, 37750852, A, G, 54906548, T, C,70912094, C, T, 87599860, T, C 7586507, A, G, 23483029, G, A, 37757594, T, G, 54925318, G, T,70955058, A, G, 84764875, C, A 1251913, C, T, 36561606, T, C, 46689230, A, C, 57372027, T, C,70503343, A, G, 84869407, A, G 1261861, T, C, 36662019, A, G, 46761650, A, C, 58730261, G, T,70565158, T, C, 861638, C, T 1432292, G, A, 36766483, A, G, 46862266, A, G, 58762145, T, C,70668853, C, T, 86213185, C, T 1468535, C, T, 36863224, T, C, 46966706, G, A, 59197697, C, T,74303057, C, A, 86266568, C, G 1587306, T, C, 39092382, T, A, 47072411, C, T, 59650367, A, C,74416996, A, G, 86377907, T, C 16432525, A, C, 39161911, T, C, 47162786, A, G, 60855381, G, T,74491884, A, G, 86990384, A, C 16462597, T, C, 39262797, A, G, 50481067, C, A, 60863526, T, C,74591748, T, C, 87061723, G, A 16564373, T, C, 3960831, C, T, 50561885, T, C, 60961855, G, T,762307, C, G, 87162954, A, G 21678548, C, G, 3969201, G, A, 50663059, C, T, 61065510, G, C,79643920, T, C, 87261779, A, G 21761675, G, A, 40959623, T, C, 51065777, C, T, 637171, C, T,79768916, T, G, 87362041, G, A 21861789, A, G, 40962855, T, C, 51243292, C, T, 65705006, C, T,79872360, C, T, 87461754, C, T 21974424, A, G, 41065140, T, C, 51286368, G, C, 65774989, C, A,79962085, C, T, 87589277, G, T 22100166, A, G, 41172917, G, C, 51445153, C, A, 65868545, A, C,80062799, A, C, 87726855, A, C 261597, C, T, 41288201, G, A, 51509696, A, T, 65966575, G, T,80886594, T, A, 87763068, G, A 31976997, A, G, 4166623, A, G, 55983532, A, G, 66063708, A, G,80964429, T, C, 87864547, G, T 32062205, C, T, 42342080, A, G, 56061863, G, A, 66180030, T, C,81069356, G, A, 87962924, G, T 32307436, C, A, 42382160, G, T, 56162090, T, C, 661888, C, T,81162736, A, G, 88257709, C, T 32434018, A, G, 42515263, T, C, 56562012, G, A, 67724682, T, C,82503830, A, G, 88262760, T, C 32595612, A, G, 45224655, T, C, 56662556, C, T, 67761911, T, C,83276473, A, C, 88361833, A, G 32722660, G, A, 45261772, G, T, 56761661, G, A, 67878611, G, C,83659743, T, C 36384967, G, T, 45364915, C, T, 57162111, T, C, 69727003, G, T,83663610, A, T 36463266, T, C, 45463008, G, A, 57303592, T, C, 69773951, G, A, 84734468, A, G Table 2 CHR02 site information (sites in CHR02 / reference bases / mutant bases) Location, ref, alt, Location, ref, alt, Location, ref, alt, Location, ref, alt, Location, ref, alt, position, ref, alt 72302866, C, A, 5098805, T, C, 26330265, G, A, 46948181, A, T,59080956, G, A, 72004311, T, C 72302869, A, C, 5107234, A, G, 26353223, A, T, 46949368, G, A,59101155, C, G, 72030317, T, G 7253491, A, G, 5127305, G, A, 26361634, C, T, 46972269, G, C,59112120, A, G, 72059291, G, T 84365973, G, T, 5151671, C, T, 26369605, C, T, 46977610, T, C,59161649, G, A, 72068035, A, G 84366003, G, T, 5164662, A, G, 26376496, A, G, 46988244, T, C,59178772, G, A, 72078164, A, G 10464610, C, T, 5222850, G, T, 26384639, C, T, 46998983, G, A,59205803, A, G, 72083243, A, G 11058709, T, C, 5267468, C, A, 26405797, A, G, 47008126, A, C,59212561, C, T, 72092140, G, A 15208993, A, G, 5277914, G, A, 26412970, A, G, 47012519, A, G,59225167, G, A, 72109161, T, A 15209014, C, G, 5283953, C, T, 26420619, C, T, 47019958, C, A,59247365, C, T, 72120692, G, T 15273308, G, A, 5288873, A, G, 26430744, T, G, 47064119, T, C,59257164, C, T, 72124028, A, T 15273923, A, C, 5296786, T, C, 26434600, T, C, 47076983, C, T,59262922, T, C, 72150032, T, G 2185294, G, T, 5307701, G, C, 26438239, G, A, 47192154, A, C,59267360, G, A, 72151350, G, A 2185304, A, C, 5308899, C, G, 26446134, A, G, 47209918, C, G,59277029, C, G, 72175517, A, T 2185495, G, A, 5318300, A, T, 26463969, T, C, 47226851, T, G,59284603, G, A, 72181701, C, T 2185597, C, T, 5324972, A, T, 26468211, T, C, 47285346, G, A,59289032, C, T, 72186795, G, A 2185529, C, A, 5330823, A, T, 26477327, A, G, 47294198, C, T,59321189, T, G, 72194934, T, C 2185717, T, G, 5340584, C, G, 26495163, G, A, 47364598, G, T,59325230, C, T, 72212906, T, A 2185727, T, C, 5344404, G, T, 26528295, G, A, 47382410, G, A,59334217, T, C, 72218029, T, C 2185759, A, G, 5375300, G, A, 26533803, C, T, 47401986, A, G,59337726, G, A, 72224540, T, C 2186585, G, C, 5380573, T, G, 26539844, A, G, 47411947, G, A,59349080, G, A, 72228987, C, T 2186692, G, C, 5404530, C, T, 26550177, A, G, 47427448, A, G,59351840, A, T, 72241802, G, A 2186882, T, C, 5412612, G, A, 26562731, G, C, 47437903, C, T,59357455, A, G, 72254921, T, C 2188076, T, C, 5417816, C, A, 26571748, C, T, 47447082, A, G,59364641, G, A, 72259837, T, A 2188230, G, A, 5425632, G, A, 26582772, A, G, 47452114, C, T,59380468, T, C, 72272333, C, A 2188299, A, G, 5429762, C, T, 26588536, G, C, 47464898, C, A,59388546, G, T, 72280026, T, C 29028881, A, G, 5435088, C, T, 26595522, C, T, 47486216, T, C,59405059, C, T, 72308782, T, A 29038304, C, T, 5447371, C, T, 26612560, T, G, 47508889, C, T,59406898, G, A, 72317276, A, G 29263625, T, G, 5449433, T, G, 26633236, T, G, 47516739, A, G,59413747, T, C, 72337057, C, G 29292753, C, T, 5479630, C, T, 26639990, T, C, 47536750, G, A,59425081, G, A, 72344638, A, G 29483180, C, T, 5508129, G, A, 26646954, T, G, 47546805, G, C,59428552, G, C, 72378736, A, T 29507390, T, A, 5538795, A, G, 26651305, T, G, 47565244, A, T,59435233, A, C, 72384519, A, G 29507584, T, C, 5541383, C, T, 26658856, A, G, 47568662, T, C,59447788, C, T, 72455375, A, C 29509014, A, T, 5568476, A, G, 26664069, T, G, 47580237, A, G,59451349, G, A, 72469060, T, G 29509760, A, G, 5578185, T, G, 26670625, T, C, 47586075, C, T,59467297, A, C, 72480735, A, C 29512515, C, T, 5587697, A, G, 26687143, G, A, 47593335, A, G,59474849, T, C, 72493592, G, C 29567127, A, G, 5592420, A, C, 26692544, G, A, 47600787, A, G,59480795, A, T, 72496604, G, A 29569732, T, G, 5596808, C, A, 26698367, C, A, 47603601, A, C,59490019, T, C, 72531170, A, C 29569775, G, C, 5610014, C, T, 26712364, C, A, 47613191, C, A,59495842, A, G, 72589108, T, G 29570204, G, A, 5614287, C, G, 26719387, A, G, 47660771, T, C,59501812, T, C, 72636848, A, T 29573405, A, T, 5620047, A, G, 26725916, G, A, 47666514, G, T,59509021, A, C, 72726979, T, C 29689669, G, A, 5625012, G, T, 26745925, G, C, 47673877, G, A,59515487, C, T, 72738985, T, C 29704562, T, C, 5638053, A, C, 26752997, T, C, 47680971, G, A,59522654, G, A, 72744779, C, T 29705599, C, A, 5658178, T, A, 26774116, A, G, 47697713, G, T,59525917, C, T, 72753636, C, G 29813844, C, T, 5673478, T, A, 26791553, A, C, 47701128, G, A,59537220, G, A, 72756241, A, G 29898776, T, C, 5678248, G, A, 26798790, T, C, 47709095, C, A,59543276, A, G, 72766087, C, T 29898891, A, G, 5685061, A, C, 26809646, A, T, 47717189, C, T,59582290, G, A, 72770628, C, T 29898926, G, A, 5690210, T, C, 26828916, T, C, 47739220, A, T,59606266, C, T, 72778227, C, T 29898907, A, C, 5694977, G, T, 26835664, G, C, 47751827, C, T,59621620, C, G, 72786735, A, G 29898989, C, T, 5732291, A, G, 26838959, T, C, 47757593, G, A,59625158, T, G, 72797404, T, C 29898950, G, T, 5740713, C, T, 26850571, T, C, 47769037, G, A,59634803, G, C, 72810556, C, T 30090796, G, T, 5746471, A, G, 26853022, G, A, 47780094, C, T,59641240, A, T, 72814867, C, T 30127588, G, A, 5756191, C, T, 26859983, T, G, 47786383, T, C,59647791, T, C, 72824863, T, G 30127964, G, A, 5768031, C, G, 26921005, A, G, 47792450, G, A,59653482, A, T, 72827209, G, A 30320446, C, T, 5772248, A, G, 26929107, A, G, 47803458, G, A,59662254, G, C, 72834302, G, A 30321022, G, A, 5780234, T, C, 26930289, T, A, 47809222, C, T,59668332, A, G, 72862578, G, A 3210249, A, G, 6165635, A, T, 26999810, C, T, 47815698, C, T,59676936, C, T, 72892829, G, A 3210259, C, T, 6183821, A, C, 27062620, A, G, 47822039, T, A,59692973, C, T, 72900074, A, T 44377532, A, T, 6191677, T, C, 27143545, G, A, 47850537, G, A,59710533, G, C, 72928735, C, T 44377583, A, G, 6195692, T, G, 27148089, C, T, 47855522, C, G,59715988, T, G, 72943024, G, C 44377572, A, T, 6209306, A, G, 27228955, G, A, 47877607, C, A,59725797, G, A, 72948085, A, T 44436620, A, C, 6221385, C, A, 27233914, G, C, 47931287, G, A,59734052, T, C, 72953332, C, T 44437369, G, T, 6223365, T, C, 27446257, G, A, 47934290, C, T,59741259, G, T, 72963430, G, C 44437393, A, T, 6230410, C, T, 27457552, A, T, 47950591, A, C,59747126, T, C, 72972567, G, A 44437410, A, G, 6248271, G, A, 27667353, A, G, 47960018, A, G,59755673, G, A, 72999451, T, C 44437551, C, T, 6251838, T, C, 27691556, T, C, 47972280, G, A,59761460, A, G, 73027992, T, C 44437626, G, A, 6259941, A, G, 27721259, T, C, 47979570, C, T,59766228, A, G, 73044522, T, C 44437597, G, A, 6267872, G, T, 27742107, C, T, 47982657, A, G,59779492, G, A, 73076583, T, A 46377505, C, A, 6273616, T, C, 27768749, G, T, 47993458, C, T,59805229, T, C, 73123383, C, A 5868378, A, C, 6287611, G, C, 28680174, A, T, 48078210, G, C,59811319, A, G, 73127201, C, T 1190720, A, G, 6309910, A, G, 28757196, G, A, 48118584, T, G,59815322, C, T, 73145328, A, G 1336914, T, C, 6322309, G, A, 28853497, G, A, 48148576, A, G,59823158, C, T, 73149782, T, G 1349670, T, A, 6327823, G, T, 28882137, C, T, 48150800, G, A,59830901, G, A, 73175251, T, C 1417462, C, G, 6363190, A, G, 29264510, T, C, 48171184, C, T,59836701, T, C, 73177018, A, G 1431395, C, T, 6371354, C, T, 29280768, T, C, 48205918, T, C,59847311, G, A, 73190866, G, A 1438403, C, T, 6380617, T, A, 29713692, C, G, 48239634, G, A,59861682, C, G, 73209680, C, A 1441829, T, A, 6383349, G, T, 30489406, G, A, 48244453, G, A,59865113, T, C, 73215811, C, A 1447649, T, C, 6394454, T, C, 30517743, C, T, 48261607, C, T,59871537, A, G, 73262787, C, A 1456273, G, A, 6400371, G, T, 30589003, A, G, 48268030, G, T,59883584, C, T, 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57609608, G, A,69908617, G, T, 37579084, A, G 3483648, A, G, 24757651, G, A, 45546460, G, A, 57620444, C, A,69965110, C, G, 37687703, G, A 3495053, A, G, 24764778, T, C, 45634614, C, T, 57626963, G, C,69986042, G, A, 37775404, G, C 3497553, G, A, 24770204, C, T, 45636783, G, A, 57634714, C, T,69990234, T, C, 3779289, G, A 3793658, G, A, 24779800, C, T, 45649396, G, A, 57637815, T, C,69997488, A, C, 37873127, G, A 3804857, A, G, 24784366, A, G, 45650846, G, A, 57656352, A, G,70095296, T, C, 37972259, C, T 3811552, G, A, 24792313, T, C, 45661832, G, A, 57662081, A, T,70101393, A, G, 38072124, G, A 3818898, T, C, 24804846, G, T, 45665357, C, T, 57668205, T, C,70112082, T, C, 38171324, T, A 3824016, A, T, 24833733, C, G, 45677537, C, T, 57684490, G, C,70114689, A, C, 38271632, A, G 3839659, A, C, 24845697, T, C, 45690277, C, T, 57689689, G, A,70125994, C, T, 38397500, C, T 3842251, C, T, 24855455, A, G, 45694282, G, A, 57693966, A, G,70131321, G, C, 38502195, G, A 3911406, A, G, 24870330, C, A, 45730340, C, T, 57714851, G, T,70147640, A, G, 38572345, A, G 3918316, A, T, 24875331, A, T, 45735774, C, T, 57739451, A, G,70153398, A, G, 38679248, T, C 4030147, C, A, 24880084, A, G, 45747267, C, T, 57745833, T, C,70174838, G, T, 38771658, T, C 4112259, C, T, 25008490, C, T, 45753786, T, C, 57749489, A, G,70180247, T, A, 39222262, C, T 4119901, G, A, 25019933, T, G, 45755930, A, G, 57760390, A, G,70189167, G, A, 39294326, G, A 4122948, G, A, 25031280, C, T, 45763057, C, T, 57777009, T, G,70192552, C, G, 39384172, T, C 4135452, C, G, 25057420, A, G, 45771199, T, C, 57780778, C, T,70201866, G, A, 39471407, T, C 4140930, A, G, 25062255, T, A, 45781014, C, A, 57788359, G, A,70210656, T, C, 39571177, G, C 4143821, T, C, 25070168, A, T, 45787004, A, C, 57792842, T, C,70217287, T, C, 39678446, A, C 4171016, G, C, 25082119, G, C, 45796992, G, A, 57804925, G, C,70223771, A, C, 39809752, A, C 4174478, T, C, 25091486, T, C, 45799328, A, G, 57809309, T, C,70238216, T, G, 39871246, A, G 4183682, A, T, 25100869, T, G, 45805770, G, A, 57813664, C, T,70250009, G, A, 39971995, G, A 4185439, T, C, 25108986, C, T, 45812485, C, T, 57823370, T, A,70264541, C, G, 40077052, A, G 4197261, T, C, 25111617, G, T, 45930052, A, C, 57831647, T, C,70275272, A, G, 40172022, T, C 4200174, T, G, 25119051, G, A, 45934351, A, G, 58011000, C, T,70280578, T, C, 44457598, A, G 4211900, C, T, 25138116, G, A, 45938683, G, A, 58032037, T, G,70322350, A, G, 44484976, G, A 4221595, C, T, 25152346, T, C, 45949802, C, T, 58041496, A, T,70332236, G, A, 44571109, T, C 4234011, T, C, 25154036, T, A, 45952188, G, A, 58081546, A, G,70343433, T, C, 44745109, A, G 4235143, T, C, 25165122, A, T, 45961957, G, C, 58097519, G, A,70352773, C, T, 44886225, T, C 4242961, T, C, 25173223, G, A, 45967485, G, A, 58108455, T, C,70459067, T, A, 49720799, A, G 4248461, T, C, 25175454, T, C, 45978845, T, G, 58117099, G, A,70476350, T, C, 49771468, A, T 4281590, C, T, 25186578, A, T, 45980671, G, T, 58175805, T, G,70570836, A, T, 49871355, A, G 4289147, C, A, 25192258, G, A, 45993465, C, T, 58211367, G, A,70602970, T, C, 51015238, A, G 4296238, C, A, 25201827, G, C, 45999641, A, G, 58251364, A, G,70612396, T, C, 51071084, G, T 4297504, C, A, 25206676, C, G, 46002590, G, A, 58257989, C, G,70613489, C, T, 51184593, A, G 4310062, C, T, 25211176, G, C, 46019369, T, C, 58265414, T, G,70632658, A, G, 57832099, G, A 4320254, G, A, 25220140, A, G, 46025360, C, T, 58285991, A, T,70643015, G, A, 57932861, C, T 4330045, C, T, 25224856, A, G, 46031151, T, C, 58294555, C, A,70672652, T, G, 57971261, A, G 4338442, G, A, 25231693, G, T, 46073437, T, C, 58302231, C, T,70684745, C, T, 5923100, G, A 4342973, G, T, 25237234, G, A, 46084321, A, G, 58304399, C, T,70748702, G, A, 6155352, C, T 4353315, A, C, 25273940, A, T, 46085686, G, C, 58320927, T, C,70786040, G, C, 71079, T, C 4358180, G, A, 25279720, T, G, 46096252, T, C, 58341918, G, A,70814249, G, A, 75852980, G, A 4374952, A, G, 25294819, T, C, 46101272, A, T, 58348160, C, A,70822376, T, A, 75945506, A, C 4386678, T, C, 25308893, A, G, 46107840, A, G, 58359385, G, A,70832569, G, A, 76032307, A, C 4393290, G, A, 25327876, G, A, 46120208, T, C, 58362360, C, T,70878805, C, T, 7717843, A, G 4398627, A, G, 25343303, T, C, 46128744, A, C, 58397151, C, T,70955977, C, T, 7814142, G, A 4402909, T, A, 25351984, C, T, 46136536, T, C, 58402600, T, C,70958515, A, T, 78814353, G, A 4412032, G, T, 25369524, C, T, 46153811, T, C, 58409091, G, C,70992025, G, C, 78872473, C, G 4425094, C, T, 25384629, C, T, 46161285, G, T, 58421011, A, G,71005751, G, T, 78973399, T, C 4431444, T, C, 25431234, T, G, 46189276, G, C, 58430845, T, C,71007890, T, C, 79076765, T, C 4518745, C, T, 25544297, G, T, 46197437, C, T, 58447513, C, T,71062993, G, A, 79241809, T, G 4529109, A, G, 25548782, T, C, 46204076, G, A, 58453332, A, C,71099053, A, C, 79278732, A, T 4530446, T, G, 25554925, G, A, 46220522, A, C, 58459491, G, A,71122184, A, G, 79482537, A, G 4548801, T, C, 25560110, A, G, 46244476, T, C, 58472193, C, T,71126744, A, G, 79580841, T, A 4560285, T, C, 25574630, T, A, 46250555, A, C, 58478613, G, T,71135721, G, A, 8018075, A, G 4567658, T, A, 25581858, C, T, 46259204, G, A, 58534037, T, G,71140066, A, T, 81161080, T, C 4578218, G, A, 25593064, C, T, 46272548, A, G, 58537119, G, A,71155435, A, G, 81173243, T, A 4583210, G, A, 25595256, T, C, 46276220, G, T, 58544156, G, A,71189042, C, T, 81282254, T, C 4586420, C, A, 25603265, C, T, 46284262, G, C, 58566973, T, G,71203152, C, T, 82680938, C, A 4598796, T, A, 25610027, A, T, 46296035, C, T, 58573803, G, A,71232007, C, A, 82790805, T, C 4604055, T, C, 25620158, C, T, 46307157, T, C, 58586900, G, A,71242657, G, A, 82878004, G, A 4618310, G, A, 25628600, C, T, 46315096, A, G, 58595175, T, C,71246678, G, A, 82973062, T, A 4627218, A, G, 25634344, C, G, 46340283, C, A, 58605035, G, A,71257093, C, T, 83112639, G, A 4628372, T, C, 25640716, A, T, 46361466, T, C, 58609704, C, T,71266200, C, T, 83181476, C, T 4638715, G, C, 25651180, T, C, 46405365, T, C, 58631781, G, A,71298768, A, G, 83291973, T, A 4709608, C, T, 25675776, G, C, 46412194, A, T, 58635258, C, T,71306240, C, T, 83392039, A, T 4731740, A, C, 25679891, T, A, 46418480, C, T, 58642357, G, A,71315403, T, G, 83474170, C, T 4735992, A, T, 25700288, C, T, 46425806, G, A, 58649713, G, A,71321117, A, G, 83571939, C, T 4743066, T, A, 25714623, C, T, 46443026, C, A, 58657490, C, G,71360747, G, A, 83675530, C, T 4752220, C, T, 25722063, C, T, 46450235, T, A, 58681928, G, A,71370540, C, A, 83787365, C, T 4757947, A, G, 25741766, A, G, 46455114, C, T, 58686052, C, T,71373680, A, T, 83871223, G, A 4766865, A, G, 25753695, T, C, 46468781, A, G, 58699699, A, C,71381205, G, A, 854184, A, G 4770245, T, A, 25769881, C, T, 46476585, C, A, 58711434, C, G,71400397, C, T, 85481728, T, G 4780246, A, G, 25776339, G, A, 46487986, T, C, 58722790, C, T,71447734, C, T, 85574261, G, A 4782074, G, A, 25778518, G, T, 46589008, A, T, 58726525, T, G,71575186, G, A, 85692770, G, A 4789987, G, C, 25794006, A, G, 46596008, A, G, 58741317, A, C,71580992, C, A, 85771589, T, C 4865638, G, A, 25889645, C, T, 46604770, C, G, 58750301, T, A,71582286, A, G, 85893662, A, T 4866979, T, C, 25897696, A, G, 46687470, A, G, 58753975, T, C,71591889, C, T, 85973877, G, A 4873590, A, C, 25910873, C, G, 46704469, G, A, 58787759, C, T,71621298, A, C, 86071933, G, A 4908534, G, T, 25913557, A, G, 46720565, A, G, 58789295, C, T,71630337, C, T, 86691930, T, C 4942223, C, T, 25936048, A, G, 46740142, G, A, 58799293, C, T,71631809, G, A, 86771585, A, G 4948964, T, G, 26042812, T, C, 46750848, C, T, 58810494, G, A,71643236, A, T, 86904700, A, G 4956751, G, C, 26048747, A, G, 46753837, A, G, 58818482, G, A,71649637, A, G, 86971659, T, C 4962172, G, A, 26057384, A, G, 46773063, A, C, 58827181, T, G,71672211, G, T, 87072136, A, G 4965341, G, A, 26058854, C, T, 46776104, T, G, 58850830, G, A,71675855, T, G, 871219, A, T 5018775, T, G, 26130979, C, T, 46780761, C, G, 58857740, C, G,71688753, T, C, 87173412, C, T 5025192, A, C, 26149449, A, G, 46797993, T, G, 58860167, G, A,71718906, C, A, 87275742, A, G 5029008, G, A, 26151322, A, G, 46810420, C, T, 58903384, C, A,71724739, G, A, 87372061, A, G 5036509, C, A, 26162986, A, G, 46822467, G, A, 58910989, C, T,71744370, T, C, 87471866, C, T 5048279, T, A, 26172240, T, G, 46823615, C, T, 58938637, T, C,71802436, A, G, 9308225, A, C 5062828, A, G, 26180262, C, T, 46832875, A, T, 59022041, T, G,71808060, G, C, 9436616, G, A 5065053, A, G, 26187801, G, A, 46847417, T, C, 59044205, C, G,71845482, G, T, 9475337, T, C 5070396, A, G, 26193365, G, T, 46861113, A, G, 59050319, C, T,71876644, C, T, 9585341, G, T 5081952, C, T, 26227009, T, C, 46868943, G, A, 59055251, A, C,71921431, T, G 5086499, G, A, 26228509, C, T, 46878463, A, T, 59063747, A, G,71939619, T, A 5096192, T, A, 26258766, C, T, 46918099, A, C, 59069770, T, A, 71958813, T, A Table 3 CHR03 site information (sites in CHR03 / reference bases / mutant bases) Location, ref, alt, Location, ref, alt, Location, ref, alt, Location, ref, alt, Location, ref, alt, position, ref, alt 74711574, G, A, 20001556, G, A, 30020802, C, T, 41465920, G, C,53701898, G, C, 63982875, T, C 28235438, A, G, 20006398, C, A, 30027436, A, G, 41475391, T, C,53726469, G, A, 63985224, T, A 29546598, C, T, 20028193, G, A, 30036142, G, A, 41488030, A, G,53745302, G, A, 64023093, A, G 30123088, G, A, 20034388, A, G, 30046043, T, C, 41505297, A, T,53753262, G, A, 64031402, C, T 30506652, C, T, 20049577, T, G, 30047989, T, C, 41513335, C, T,53757157, T, C, 64040109, A, T 30506664, C, T, 20055644, A, G, 30057583, A, T, 41516778, T, A,53762519, T, C, 64041839, A, G 30512390, G, A, 20065893, G, A, 30064670, G, T, 41524962, G, A,53774304, T, G, 64049546, T, C 30512750, C, A, 20073753, A, G, 30071149, T, G, 41533718, C, T,53781101, A, G, 64055164, G, A 30512856, T, C, 20079628, T, A, 30404189, G, A, 41538621, C, T,53787603, C, T, 64065772, C, T 30512909, G, A, 20089480, G, A, 30466257, C, T, 41548991, T, C,53789792, A, G, 64103779, C, T 30513022, G, A, 20092873, C, T, 30482079, C, T, 41566604, G, C,53814382, T, C, 64110424, G, A 30513129, A, G, 20096978, C, T, 30483361, T, C, 41572279, C, T,53839918, A, G, 64112916, G, A 30513906, G, A, 20108251, C, G, 30550939, A, T, 41585416, A, G,53850544, T, C, 64134843, A, G 30526922, T, C, 20114910, C, T, 30559543, G, A, 41604477, C, T,53854295, C, T, 64185527, T, C 30526933, G, A, 20122342, T, C, 30565502, C, T, 41617567, G, T,53864358, C, G, 64203638, A, T 30544327, C, T, 20156715, T, A, 30570212, C, T, 41639228, C, T,53867240, C, T, 64236325, A, G 31589120, C, T, 20183390, C, T, 30583944, G, A, 41673866, G, A,53877296, C, T, 64247297, C, T 32618249, A, G, 20195227, C, T, 30588610, C, T, 41681340, G, C,53883983, A, T, 64254782, T, C 32618300, G, A, 20212456, A, C, 30620128, G, A, 41683326, A, T,53889626, G, A, 64261397, T, C 32620304, T, C, 20219090, G, A, 30636186, T, G, 41722496, G, C,53899762, A, T, 64267820, T, C 32620390, A, C, 20227719, A, G, 30639381, C, T, 41746377, G, C,53904412, C, T, 64297749, A, G 33130311, A, C, 20244010, A, G, 30644476, T, C, 41756306, A, T,53910550, C, T, 64303954, A, G 37657663, A, G, 20252895, A, C, 30654448, C, T, 41760484, C, A,53917324, G, A, 64309733, C, T 38188437, C, T, 20258766, T, C, 30664337, A, G, 41770260, A, G,53926271, T, G, 64316711, A, C 38188451, G, A, 20286462, A, G, 30670126, G, A, 41780943, A, G,53939863, C, T, 64322834, C, T 38196706, A, G, 20292670, A, G, 30678007, G, A, 41796260, G, A,53962235, C, T, 64331145, A, G 46488335, G, A, 20313455, T, C, 30685431, T, C, 41808096, T, G,53971387, A, G, 64342970, T, C 46488719, G, T, 20315419, A, G, 30720427, T, C, 41813625, C, G,53980740, G, A, 64359878, T, C 46615653, A, C, 20349306, G, A, 30726801, A, T, 41853604, A, G,54089907, A, G, 64369900, C, A 47506984, T, C, 20409913, G, C, 30733382, T, A, 41864039, C, T,54106201, A, G, 64376246, T, C 70374528, T, A, 20413016, T, G, 30735355, A, G, 41871937, C, A,54209413, G, A, 64378163, T, C 70374648, C, T, 20422219, C, T, 30753857, A, C, 41887913, G, T,54221909, C, A, 64404882, A, G 70374678, A, G, 20429589, G, A, 30762224, G, C, 41899035, C, T,54224545, T, C, 64411618, T, G 70427150, G, T, 21152605, A, G, 30767892, T, C, 41902245, A, G,54248732, A, C, 64419684, C, T 70476649, G, A, 21180853, G, A, 30773392, C, T, 41909334, T, C,54256408, C, T, 64423604, C, T 70476678, C, T, 21203913, T, G, 30779764, C, T, 41927921, A, G,54259031, C, T, 64431997, T, C 71590979, G, A, 21208207, C, A, 30790951, C, A, 41932427, A, G,54272078, T, C, 64439819, G, A 72338450, T, A, 21220901, C, G, 30794261, G, C, 41936153, T, G,54276767, A, G, 64446482, G, A 72446892, C, G, 21229914, G, A, 30806113, C, T, 41943716, T, C,54314604, C, G, 64453322, C, T 72446979, T, C, 21239165, A, G, 30813246, A, G, 41950627, C, G,54320394, A, C, 64459799, T, C 72447030, A, G, 21242799, G, T, 30824113, G, A, 41957033, G, T,54329257, A, G, 64464592, T, C 10003269, A, G, 21256229, C, G, 30832838, A, C, 41966400, A, C,54351707, C, T, 64472345, T, C 10007675, C, G, 21269651, T, C, 30845710, T, G, 41980493, T, G,54359257, T, G, 64551677, C, T 10014897, T, C, 21282484, A, C, 30859199, A, G, 42020128, G, A,54365833, G, C, 64559920, G, A 10023016, A, G, 21290048, G, T, 30867776, A, G, 42030487, C, T,54388441, T, G, 64565192, A, G 10030841, G, A, 21295450, C, A, 30869160, T, C, 42040343, T, A,54395228, C, T, 64583806, A, G 10042162, A, G, 21330196, T, A, 30881047, C, T, 42042471, T, C,54401652, G, C, 64592659, A, C 10057188, T, C, 21346488, C, T, 30887185, C, T, 42054112, C, T,54410604, A, G, 64600924, T, C 10063972, C, T, 21388017, G, A, 30889882, A, G, 42068704, G, A,54420396, A, C, 64607972, C, T 10082350, G, C, 21389383, A, G, 30908181, G, C, 42076768, A, T,54422620, A, G, 64612701, A, T 10086282, T, C, 21402695, G, T, 30911236, G, A, 42083873, T, C,54429194, G, A, 64681972, C, T 10096593, A, T, 21406219, A, C, 30924083, A, G, 42103181, A, G,54441426, T, C, 64692072, G, A 10115511, A, G, 21423184, T, C, 30931223, T, C, 42105005, T, A,54444745, A, G, 64719841, C, T 10128367, G, T, 21426559, C, A, 30933519, C, T, 42114824, C, T,54449819, T, C, 64723635, T, C 10135602, T, C, 21439336, G, C, 30940618, C, T, 42123761, G, A,54457214, T, G, 64737897, G, A 10144928, G, A, 21449928, G, C, 30947040, T, C, 42125399, A, G,54520227, C, T, 64746986, A, G 10149919, A, G, 21470824, G, A, 30964099, A, G, 42135368, T, C,54547221, A, T, 64750504, T, C 10154160, G, A, 21480532, T, A, 30979562, T, G, 42153679, C, A,54644561, T, A, 64774608, C, T 10172178, T, C, 21500030, G, A, 30985125, C, T, 42180264, G, C,54648446, G, A, 64779735, G, A 10175663, A, G, 21503785, C, T, 30994500, A, C, 42193881, A, G,54656498, T, C, 64790963, A, T 10215945, G, A, 21518320, A, G, 31006858, C, T, 42198824, G, A,54660634, C, G, 64796041, G, A 10238464, T, C, 21535702, A, C, 31012899, A, G, 42221601, A, G,54666457, T, C, 64800094, A, T 10269177, T, C, 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52162830, T, C,61317634, A, G, 28506374, C, T 18546620, A, G, 28051657, A, T, 39958988, A, T, 52172232, C, T,61324767, C, T, 28607960, T, A 18552817, G, A, 28108633, T, C, 39975124, C, A, 52177214, G, C,61330187, C, T, 28704206, C, G 18559677, A, T, 28112843, C, G, 39978525, C, G, 52185086, T, C,61356332, A, G, 2905776, C, A 18568852, C, T, 28184039, A, G, 39991421, A, C, 52190290, C, A,61362198, T, C, 29626621, C, T 18576901, A, C, 28188638, T, C, 40004456, G, A, 52198415, G, C,61371431, A, G, 29758549, G, A 18620451, G, A, 28206250, A, G, 40021219, A, C, 52206491, G, C,61377798, G, T, 29827634, G, A 18634109, A, C, 28220455, T, C, 40043122, A, G, 52209700, C, G,61405219, T, C, 29927806, T, A 18646798, A, T, 28223707, T, C, 40052590, G, A, 52217323, G, A,61412434, A, G, 3004431, C, T 18657023, C, T, 28236446, G, A, 40054069, C, T, 52223236, T, C,61418398, G, A, 30217890, T, G 18666119, A, T, 28413539, A, G, 40085534, T, C, 52232576, G, A,61426440, C, T, 30331344, T, C 18674599, C, A, 28445303, A, C, 40113910, G, C, 52238968, G, A,61431013, T, G, 3127069, G, C 18681268, G, T, 28446706, C, G, 40123037, T, G, 52248604, G, A,61435869, C, T, 3204298, C, T 18691199, C, T, 28458352, A, T, 40125275, T, C, 52262424, C, T,61446355, C, G, 3315516, A, C 18697051, A, G, 28465161, G, A, 40131842, C, G, 52268886, C, T,61453319, G, A, 3423891, A, G 18699839, C, A, 28467736, G, T, 40162249, G, A, 52275354, T, C,61457255, G, T, 3504194, A, G 18712866, T, C, 28480415, T, A, 40166999, C, T, 52279315, C, T,61464269, C, T, 3610455, A, G 18717736, G, A, 28481695, A, C, 40173824, C, T, 52314930, C, T,61483642, T, C, 36930640, G, T 18722030, T, C, 28725051, G, A, 40184475, T, C, 52330558, A, T,61507277, C, A, 3704308, T, C 18728948, G, A, 28739227, G, C, 40196306, G, A, 52342341, C, T,61515399, T, C, 37070898, G, A 18739252, T, C, 28747251, C, A, 40201485, A, C, 52349154, G, A,61525228, C, T, 37692274, T, C 18743145, C, A, 28749029, G, A, 40215293, A, G, 52356433, T, C,61531138, T, A, 37738132, G, T 18765084, G, A, 28757216, T, G, 40225767, T, A, 52366520, A, C,61539278, C, T, 37866977, A, C 18770459, A, G, 28768923, T, G, 40229265, T, C, 52381916, C, T,61553733, C, G, 3805116, A, C 18781432, A, G, 28778816, T, C, 40241958, C, T, 52389700, C, T,61557411, A, G, 38846121, A, G 18784277, T, C, 28787771, A, G, 40243127, C, T, 52392821, G, T,61595328, T, C, 38904273, G, A 18814582, A, G, 28790421, T, C, 40255167, T, G, 52410269, G, A,61597020, A, G, 39004796, T, C 18824509, T, C, 28800520, T, A, 40276843, C, T, 52416824, A, C,61605988, G, A, 3904268, G, T 18828032, A, G, 28805149, G, A, 40298721, C, T, 52419361, C, T,61649376, G, A, 39105077, A, T 18834738, T, C, 28813102, T, C, 40308792, T, C, 52541951, T, C,61666224, T, G, 39206482, T, C 18843905, C, T, 28843382, T, C, 40317584, T, G, 52549881, T, C,61668769, C, T, 39304348, C, G 18850045, G, A, 28851562, C, T, 40333932, A, G, 52557320, T, C,61677641, G, T, 4011522, T, C 18860908, A, T, 28857731, T, C, 40340220, C, T, 52568625, T, C,61687832, G, T, 4113608, T, G 18864289, G, A, 28862433, G, A, 40347775, A, T, 52579816, T, G,61689950, C, T, 4205319, A, T 18871573, C, T, 28878460, C, T, 40354214, C, A, 52584924, A, G,61699746, G, A, 42261559, A, G 18885125, G, A, 28900281, G, A, 40367203, C, T, 52591259, A, G,61706144, G, T, 42315291, G, A 19053513, G, A, 28904337, C, G, 40408935, T, A, 52599777, C, T,61720738, C, T, 42404668, A, T 19062234, A, C, 28935980, A, G, 40420883, A, C, 52601978, G, A,62049019, A, G, 42504409, T, C 19092329, C, T, 29019306, G, T, 40554041, G, A, 52685319, T, G,62188564, T, G, 4304281, A, G 19097260, T, C, 29026617, T, A, 40570388, T, C, 52687462, G, C,62205127, C, T, 4425123, A, T 19103525, A, T, 29029396, T, C, 40575138, C, T, 52696152, C, A,62208543, A, T, 4506906, G, A 19108247, A, G, 29036872, C, T, 40586980, T, C, 52702173, T, C,62217785, G, A, 4647487, A, C 19121777, A, G, 29045332, C, A, 40598242, G, A, 52709509, A, T,62228617, C, T, 4706478, T, A 19184815, T, A, 29054276, A, T, 40604436, T, C, 52727733, G, A,62231441, A, G, 48079670, G, C 19210215, C, G, 29059512, A, G, 40613658, T, C, 52744124, G, T,62241234, T, A, 4811405, A, C 19215845, A, G, 29070724, C, T, 40618874, C, T, 52956346, C, T,62243423, C, G, 48151517, A, T 19221082, T, C, 29074115, G, C, 40626089, C, T, 52966020, G, T,62276844, C, A, 48235848, G, A 19237529, G, T, 29081443, G, A, 40657000, G, T, 52972089, C, T,62278386, G, A, 48535561, C, T 19243754, A, G, 29089730, A, C, 40692322, C, A, 52983530, G, A,62288482, T, G, 4904389, T, A 19249665, C, T, 29103504, C, T, 40702974, C, T, 53003757, T, C,62296441, T, C, 49784956, T, C 19254352, G, C, 29107969, G, C, 40709002, T, C, 53036878, A, T,62316814, A, C, 50084499, A, G 19273113, G, A, 29114601, A, G, 40733147, C, A, 53057538, C, T,62367579, C, T, 50111864, G, T 19276769, C, T, 29123296, C, T, 40739361, C, T, 53063932, T, A,62421648, C, T, 50304013, C, T 19283630, T, C, 29131343, T, A, 40742476, A, G, 53070285, C, T,62426007, C, A, 50892758, C, T 19291977, C, T, 29134765, A, C, 40755528, A, C, 53075865, G, A,62437477, T, G, 50904360, A, C 19305538, C, T, 29147131, A, G, 40762954, G, A, 53092982, G, A,62447994, G, A, 5097460, C, T 19316627, A, G, 29148793, G, T, 40774602, T, C, 53098005, A, G,62459185, G, A, 51007554, T, C 19319486, T, C, 29161116, C, T, 40779055, G, A, 53114238, G, A,62461652, T, C, 5106570, G, C 19357290, A, G, 29169229, G, T, 40785808, C, G, 53115950, C, T,62488589, A, G, 51113080, G, T 19365976, C, T, 29173774, A, G, 40791302, C, T, 53131569, G, A,62498713, A, T, 51233140, T, G 19367302, A, G, 29205495, T, C, 40804116, T, G, 53136229, G, T,62504510, C, T, 51330103, T, C 19380101, T, G, 29216100, C, T, 40816650, C, T, 53142692, T, G,62515577, C, A, 51406791, G, A 19386051, C, A, 29218641, G, C, 40823385, T, G, 53173453, A, G,62519495, G, C, 5205991, A, C 19392384, A, G, 29239295, G, A, 40826179, A, G, 53180735, T, C,62529461, A, C, 52744146, T, G 19401035, C, T, 29252974, G, A, 40839908, T, C, 53197125, C, T,62536017, A, G, 52808062, G, C 19405526, G, A, 29254298, G, T, 40845606, G, A, 53205155, C, G,62543436, A, G, 52904482, A, G 19409775, C, T, 29265738, A, G, 40853411, G, A, 53210934, T, C,62545013, T, G, 5346182, G, A 19418145, A, G, 29273287, A, G, 40856774, G, A, 53214785, C, T,62557691, T, C, 58081325, A, G 19424829, G, A, 29279641, G, A, 40861632, G, A, 53220594, T, G,62559247, G, A, 58104283, C, T 19431803, T, G, 29293544, C, T, 40868246, A, G, 53231309, A, G,62589110, T, C, 58206387, C, T 19450777, T, A, 29301902, C, A, 40878703, T, C, 53246735, T, A,62599836, T, C, 61842622, G, A 19460199, C, G, 29307924, A, G, 40908680, G, C, 53253586, T, C,62606708, T, C, 61984699, C, T 19471813, A, C, 29313888, C, G, 40911412, G, A, 53255695, G, A,62609441, T, C, 62044713, G, A 19472820, G, C, 29321109, C, T, 40928500, A, C, 53310308, A, G,62707826, A, G, 62722460, T, A 19483504, T, A, 29329089, C, T, 40943348, C, T, 53343866, T, C,62714604, T, C, 62951269, C, G 19488317, A, G, 29335381, T, A, 40948879, G, A, 53352398, G, A,62721558, A, C, 63042839, A, G 19580822, G, C, 29339030, C, T, 40959143, A, G, 53364880, G, T,63407284, C, T, 63163287, T, G 19590626, C, G, 29355692, T, C, 40964478, C, T, 53370222, A, G,63418495, A, G, 66557545, A, T 19593899, T, C, 29360921, C, T, 40976917, T, C, 53380424, G, A,63424047, G, A, 66698506, C, T 19601863, T, A, 29366164, A, T, 41007679, G, A, 53385598, T, A,63436065, T, A, 66758953, G, A 19605940, A, G, 29374979, G, A, 41008842, T, G, 53393070, G, A,63438699, T, G, 67144317, T, A 19615231, T, C, 29384805, C, T, 41016820, C, A, 53395573, G, A,63444254, C, T, 68629081, G, A 19632454, T, A, 29406012, G, A, 41050247, A, G, 53406303, A, G,63478210, T, G, 68898374, T, C 19645435, A, G, 29411622, T, C, 41052763, G, A, 53418110, C, A,63481049, T, G, 6947522, T, C 19649455, A, G, 29418510, G, C, 41060080, A, G, 53424799, T, C,63489481, C, T, 69632856, C, T 19670294, C, G, 29425719, C, T, 41068602, C, T, 53431273, A, G,63495360, T, C, 69808046, A, G 19706299, T, G, 29447933, A, G, 41072186, G, A, 53439963, G, C,63501979, G, C, 70050243, C, T 19721596, C, A, 29452976, G, A, 41079906, G, A, 53445158, C, T,63509547, T, C, 7010232, G, T 19770193, G, A, 29462655, T, A, 41085928, A, C, 53455337, C, T,63527847, T, A, 70153927, C, T 19778928, T, C, 29464519, A, G, 41092944, C, T, 53472339, G, T,63542971, G, A, 70871551, T, A 19784109, G, C, 29473269, T, G, 41108250, C, T, 53473806, A, T,63553937, A, T, 71246668, C, T 19790339, G, A, 29482938, T, A, 41155556, A, G, 53479886, A, T,63560445, C, T, 7158139, A, G 19795539, A, G, 29494598, T, C, 41160608, A, G, 53493260, G, C,63583911, A, C, 71681430, G, A 19808693, T, C, 29509123, C, G, 41166917, A, C, 53511554, A, G,63617219, T, G, 71764181, C, T 19818761, G, A, 29516159, A, G, 41170426, C, T, 53526190, C, T,63627416, T, C, 71873173, G, A 19842090, G, T, 29524854, G, C, 41179400, T, C, 53529755, T, C,63638001, A, T, 74979051, T, C 19848125, G, C, 29530015, G, T, 41187560, T, A, 53536536, C, G,63680068, A, G, 75004216, T, C 19865232, T, C, 29540974, T, G, 41193979, C, T, 53544974, T, G,63693548, A, T, 7914761, A, G 19869106, C, A, 29547617, A, G, 41203814, T, C, 53556792, A, G,63711709, T, C, 8034368, A, G 19876635, A, G, 29587807, T, G, 41206314, A, G, 53561510, G, A,63728251, A, G, 8128624, T, C 19886208, G, A, 29940991, G, A, 41218636, A, T, 53586088, C, T,63773759, A, G, 8205499, C, A 19889583, G, C, 29943456, A, C, 41224254, C, T, 53595226, T, A,63809995, C, T, 9480167, A, G 19901655, T, C, 29950379, A, T, 41232032, A, T, 53611254, G, C,63862773, A, G, 9504390, G, A 19907813, A, G, 29959673, C, T, 41242639, C, T, 53616614, A, T,63870964, A, G, 9646734, C, T 19923817, T, C, 29968806, C, T, 41247313, C, G, 53626104, A, C,63878674, G, A, 9705327, C, T 19961623, C, A, 29990941, G, T, 41260814, T, A, 53633497, A, T,63908116, G, A, 9816209, C, T 19972484, A, T, 29992859, T, C, 41281806, T, A, 53635022, T, A,63926115, A, G, 9904169, A, G 19979570, G, T, 30002052, T, C, 41383054, G, C, 53641947, A, T,63932895, T, C 19988655, A, G, 30008942, A, G, 41440347, G, A, 53653977, T, C,63935783, C, T 19995085, C, G, 30012186, T, C, 41445669, C, T, 53671720, A, G, 63956156, A, G Table 4 CHR04 site information (sites in CHR04 / reference bases / mutant bases) Location, ref, alt, Location, ref, alt, Location, ref, alt, Location, ref, alt, Location, ref, alt, position, ref, alt 51385316, T, A, 16200933, C, G, 19717893, T, A, 31244136, G, A,46478280, A, G, 56839517, G, A 54430522, C, A, 16210036, A, G, 19734816, C, T, 31278951, G, A,46480843, A, G, 56844490, A, G 54865792, G, C, 16218201, G, A, 19742204, T, C, 31341166, G, A,46492081, A, G, 56852212, A, G 56904310, C, T, 16223985, T, C, 19752517, T, C, 31458179, G, A,46495439, T, C, 56864018, A, G 2421949, C, A, 16231500, C, T, 19760179, G, A, 31485627, C, G,46510840, T, C, 56870515, T, C 2422003, T, C, 16240621, A, G, 19770235, T, G, 31490703, C, T,46526630, T, A, 56897524, A, G 2683023, T, C, 16247882, G, A, 19776112, T, C, 31496361, C, T,46548181, T, G, 56950536, A, G 2683206, T, G, 16252234, T, C, 19796468, T, C, 31501179, A, G,46579818, A, G, 56957400, G, A 2683264, C, T, 16257640, G, T, 19807784, G, C, 31528671, T, C,46597092, C, T, 56996713, C, T 2683277, G, C, 16269060, C, G, 19810340, C, A, 31535100, C, A,46610244, G, A, 57001568, G, A 2683344, C, T, 16271702, C, G, 19868284, G, T, 31541844, A, G,46643479, A, C, 57025176, A, G 2683356, A, G, 16298416, T, C, 19876188, G, A, 31591474, C, T,46697124, C, T, 57031330, T, C 2683461, C, T, 16305130, G, A, 19881731, C, T, 31625092, T, C,46703327, A, G, 57035153, C, T 29246595, T, A, 16318121, T, C, 19951779, C, T, 31634014, G, T,46714279, A, C, 57044966, A, T 29246722, A, G, 16322079, G, A, 19966077, C, T, 31646189, A, G,46721762, C, T, 57054135, C, T 29246736, G, A, 16327224, G, T, 19976411, A, G, 31651719, C, T,46729249, T, C, 57070710, T, C 29246944, T, C, 16337137, C, T, 19981986, A, G, 31679767, A, G,46743046, C, T, 57081049, C, G 29247000, A, G, 16380604, G, A, 19988422, A, G, 31687924, T, C,46747930, A, G, 57086227, C, T 48408198, G, A, 16389241, G, C, 19996430, G, A, 31690580, C, G,46756140, T, C, 57092654, G, A 70777151, T, C, 16395754, T, A, 20020936, A, G, 31810938, A, T,46764861, G, A, 57121321, G, A 70777162, C, T, 16400326, T, C, 20029794, G, A, 31817207, A, T,46773517, A, T, 57126582, G, A 70777177, G, A, 16404251, G, A, 20035296, G, A, 31828105, C, T,46777563, G, A, 57132180, A, T 70777205, G, A, 16410737, T, A, 20088481, C, T, 31837469, G, A,46797953, C, T, 57159593, T, C 70777680, T, C, 16419970, G, T, 20106955, T, C, 31841464, G, A,46821119, T, C, 57168616, A, G 70778897, A, G, 16425593, C, G, 20111110, T, C, 31848741, T, A,46826107, T, C, 57175087, A, G 70779371, A, G, 16518542, G, A, 20118330, A, G, 31866787, T, A,46830357, T, C, 57197735, G, A 70779382, C, T, 16538133, C, T, 20126437, T, C, 31889065, A, G,46837622, G, A, 57210283, T, C 70779395, A, T, 16547448, G, A, 20132294, G, A, 31895936, G, A,46852943, T, C, 57228653, C, T 70779431, T, C, 16556141, C, A, 20144048, C, T, 31907105, C, T,46878183, A, G, 57232777, A, G 70780233, G, T, 16588576, A, G, 20152608, T, C, 31909637, C, T,46885166, A, G, 57242417, A, T 70781124, A, G, 16662635, A, G, 20153733, A, G, 31920112, G, A,46887300, G, A, 57248488, A, T 70781893, A, G, 16668076, T, G, 20160407, G, A, 32086429, A, G,46905829, A, G, 57256760, A, G 70782333, C, T, 16681316, C, T, 20180475, A, G, 32228398, T, C,46909275, T, C, 57257874, A, G 70782360, A, G, 16695397, A, G, 20181546, A, G, 32255843, G, A,46989493, T, C, 57268206, A, G 70783347, A, G, 16704892, A, C, 20200941, A, T, 32404157, T, C,46997413, C, T, 57274848, T, C 70783359, A, G, 16707372, C, T, 20221915, G, A, 32684838, T, G,47001356, T, A, 57283846, T, C 70783410, T, C, 16719062, C, G, 20228511, A, G, 32724747, T, G,47010791, T, C, 57296426, T, C 70783464, G, A, 16726146, T, C, 20230837, C, T, 36840146, T, G,47020700, G, A, 57306641, T, C 70783476, T, A, 16728010, G, A, 20241199, A, G, 36882582, A, T,47046823, T, C, 57339821, G, T 70783503, T, C, 16749194, G, A, 20251446, C, T, 36891827, C, A,47054246, A, G, 57348333, A, G 70783546, T, C, 16759333, A, C, 20254921, A, G, 36920994, A, G,47062019, A, G, 57349757, T, C 70783713, A, G, 16766800, A, T, 20261770, A, G, 37005906, C, T,47079725, A, C, 57358469, A, G 70783793, T, G, 16770814, T, C, 20267856, G, A, 37011809, G, A,47110594, C, T, 57362756, T, C 70783804, G, A, 16776816, A, C, 20290786, C, G, 37038756, G, A,47116365, G, A, 57376615, C, T 70785048, A, G, 16785355, A, G, 20335351, A, T, 37045963, T, C,47119534, A, G, 57381392, G, A 70785261, C, T, 16803282, C, T, 20337474, T, C, 37152637, G, A,47157072, A, C, 57402918, C, G 70785311, G, A, 16809819, G, C, 20350154, A, T, 37319546, G, A,47164631, G, A, 57407142, G, T 70785698, A, T, 16815329, A, C, 20362745, G, A, 37330937, G, A,47172810, G, T, 57416978, G, C 7641190, T, A, 16822386, T, G, 20364323, T, A, 37341194, G, 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G,53956134, C, A, 68946259, G, A 13383579, C, A, 8338551, C, T, 27445953, A, T, 44264398, G, A,53961780, T, A, 69026620, A, T 13388802, G, A, 8370242, T, C, 27450580, T, G, 44276082, A, C,53966479, T, C, 69067217, G, A 13405956, T, C, 8382325, A, C, 27462440, C, T, 44281676, A, C,53972808, C, T, 69119212, C, A 13413875, A, T, 8390390, T, C, 27473602, C, T, 44288385, T, C,53982594, A, G, 69136246, C, T 13448538, T, C, 8399984, G, A, 27477771, T, A, 44308332, C, A,54001145, A, G, 69139284, C, G 13472866, A, T, 8406484, T, C, 27486944, C, T, 44325939, A, G,54012666, T, C, 69238424, C, A 13477889, T, C, 8414908, G, A, 27497471, A, C, 44333256, T, C,54016476, A, G, 69265225, A, G 13484549, T, C, 8419712, G, A, 27501080, A, G, 44341723, A, G,54040931, C, A, 69314844, G, C 13495696, G, A, 8438447, T, G, 27508934, T, G, 44347791, G, T,54046932, G, A, 69327298, G, A 13498225, A, G, 8442056, G, A, 27514775, T, G, 44376654, T, C,54091099, T, C, 69352079, G, T 13507852, G, T, 8456265, C, T, 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G,54432253, T, C, 69632617, G, A 13732131, G, T, 8983044, T, A, 27774633, A, G, 44622544, A, T,54435072, C, T, 69665009, A, G 13739847, G, A, 9040515, G, A, 27779235, C, G, 44626097, G, A,54448278, C, T, 69666804, C, T 13755120, T, A, 9057841, C, T, 27806260, T, C, 44633515, T, C,54450623, T, C, 69673509, C, A 13759330, C, A, 9073079, G, T, 27825245, A, G, 44638893, G, T,54462377, T, C, 69748687, A, G 13768682, T, C, 9087261, A, C, 27831353, C, T, 44647816, G, A,54468124, A, C, 69752244, G, A 13774338, T, A, 9096604, G, A, 27853052, G, A, 44659082, T, C,54469816, A, C, 69766723, G, A 13781953, G, T, 9112174, C, T, 27861643, A, C, 44664513, G, A,54504307, A, G, 69771754, C, T 13787973, G, A, 9120188, A, T, 27869408, T, G, 44670794, T...

Claims

1. A combination of SNP sites in swamp eels, characterized in that... This SNP locus combination consists of 44,832 SNP loci. The location information of these 44,832 SNP loci was determined by sequence alignment based on a reference version of the swamp eel genome, GWHBEHV00000000. The 44,832 SNP loci are distributed on the 12 chromosomes of the swamp eel, and the information of these 44,832 SNP loci is shown in Tables 1 to 12 of the instruction manual.

2. The combination of SNP sites of the swamp eel according to claim 1, wherein the 44,832 SNP sites include 44,793 background SNP sites and 39 foreground SNP sites.

3. A liquid phase chip for high-yield breeding of swamp eels (40K), comprising probes for detecting the 44,832 SNP sites as described in claim 1, wherein each SNP site corresponds to two single-stranded nucleotide probes for specific detection of that site.

4. The method for preparing the 40K high-yield breeding liquid phase chip for swamp eels according to claim 3, comprising the following steps: S1. Extract DNA from the eel and resequencing it; S2. The resequencing results were compared with the reference version of the swamp eel genome, and genotyping was performed; S3. Filter to obtain the background SNP site set, and filter out the background SNP sites that are evenly distributed on the chromosome from the background SNP site set; S4. Based on the association with important traits of the swamp eel, prospective SNP sites were screened from the SNP sites; S5. Design probes for detecting SNP sites targeting both background and foreground SNP sites; In step S3, the screening criteria for background SNP sites are: biallelic SNPs; MAF ≥ 0.05; deletion rate ≤ 10; and heterozygosity rate ≤ 50%. In step S4, the screening of prospective SNP sites was performed using GWAS software for genome-wide association analysis; In step S5, probe synthesis is further based on the following principles: probe length 110 bp; probe GC content 30-70%; maximum number of similar fragments ≤ 5.

5. A method for applying the 40K high-yield breeding liquid phase chip of the swamp eel described in claim 3 to swamp eel genotyping, characterized in that... It includes the following steps: S1. Extract DNA from the swamp eel and construct a high-throughput sequencing library; S2. DNA fragments containing target SNP sites in a library were captured using a 40K high-yield breeding liquid phase chip for the swamp eel, and then amplified and purified. S3. Perform high-throughput sequencing and align the results to the reference genome of the yellow eel (reference version GWHBEHV00000000) to obtain the genotype of the yellow eel to be tested at the target SNP site.

6. The application of the SNP site combination of the yellow eel as described in claim 1 in the genotyping of yellow eels.

7. The application of the SNP site combination of the yellow eel as described in claim 1 in the selection of the yellow eel genome.

8. The application of the 40K high-yield breeding liquid phase chip of swamp eel as described in claim 3 in swamp eel genotyping.

9. The application of the 40K high-yield breeding liquid phase chip of swamp eel as described in claim 3 in the selection of swamp eel genome.