Weighing dilution apparatus for preparing media and methods relating thereto

Abstract

The invention relates to a weighing device for preparing a culture medium, called device. The device comprises a weighing scale comprising a weighing table, at least two liquid pumps arranged to generate a flow of liquid to be injected into the weighing table, respectively, and a control unit of the at least two liquid pumps. The control unit is arranged to control each pump, respectively and independently, and to calibrate each pump, respectively and sequentially, according to the mass of liquid injected and weighed by the weighing scale per unit of time, then to implement a succession of serial dilutions, called serial dilutions, according to the calibration data, by starting at least one pump communicating with a reservoir of concentrated solution of culture medium during the operation of at least another pump communicating with a reservoir of diluent.

Description

Technical Field

[0001] This invention relates to the preparation of culture media for culturing microorganisms.

[0002] This invention covers research laboratories as well as analytical and / or quality control laboratories.

[0003] This invention is particularly relevant to the food, medical, cosmetic, chemical, or pharmaceutical industries. Background Technology

[0004] Culture media are carriers used to cultivate microorganisms such as cells, bacteria, yeast, and mold in the laboratory.

[0005] Culture media are typically sterile and consist of aqueous solutions, particularly those containing minerals, glucose, pH buffers, and growth factors.

[0006] Each culture medium is specifically formulated for the growth of a particular type of microorganism. The medium has precise components. These components contain precise amounts of minerals and glucose, a specific pH buffer, and specific growth factors suitable for the target microorganism to be cultured.

[0007] Preparing culture media is a necessary and prerequisite step for culturing microorganisms.

[0008] Ready-made culture medium solutions are available commercially. These solutions are packaged in plastic bottles, plastic bags, plastic bladders, or plastic pouches.

[0009] However, when conducting the same research or experiment, large-scale microbial cultures must be carried out in parallel. Furthermore, the amount of culture medium required in a single research laboratory is enormous, potentially ranging from a few dozen liters to several hundred liters per day.

[0010] Plastic bottles, bags, bladders, or pouches containing culture medium solutions, with capacities ranging from several hundred milliliters to a few liters, are designed for easy handling by operators.

[0011] In addition, using currently available culture medium solutions would lead to a significant consumption of plastics.

[0012] In addition, the use of commercially available culture medium solutions has led to the laboratory regularly replenishing large quantities of culture medium.

[0013] One object of the present invention is to overcome at least one disadvantage of the prior art.

[0014] Another object of the present invention is to provide a weighing and dilution apparatus for preparing culture media, which achieves: • Reduce environmental impact, and / or • Reduce the amount of plastic required for microbial culture, and / or • Reduce the transport volume and / or mass when providing culture medium solutions, and / or • Reduce the preparation time of the culture medium solution, and / or • Automated preparation of culture medium solutions. Summary of the Invention

[0015] Therefore, the present invention relates to a weighing and dilution apparatus for preparing culture media (hereinafter referred to as "the apparatus of the present invention").

[0016] The device of the present invention includes a weighing scale.

[0017] The apparatus of the present invention includes a weighing platform.

[0018] The weighing scale is configured to weigh the mass that falls onto the weighing platform.

[0019] Preferably, the weighing scale includes, supports, is used for or configured to support the weighing platform, and / or the weighing platform is used for or configured to be placed on the weighing scale.

[0020] In this instruction manual, "culture medium" can be understood as: culture medium solution.

[0021] The device of the present invention also includes at least two liquid pumps.

[0022] The at least one liquid pump, preferably, is configured to generate liquid flow.

[0023] Preferably, the liquid flow generated by the liquid pump, preferably the liquid flow generated by each liquid pump, is used to fall on and / or flow out and / or inject into the weighing platform.

[0024] Preferably, the liquid flow generated by the pump, after flowing out of and / or into the weighing platform, falls onto the weighing platform. In other words, preferably, the liquid flow falling onto the weighing platform corresponds to and / or constitutes and / or is the liquid flow generated by the pump.

[0025] At least one liquid pump, preferably, the inlet of the at least one liquid pump is connected to the culture medium concentrate storage tank, and the connection method is preferably jet type or fluid connection method.

[0026] At least one other liquid pump, preferably the inlet of which is used to connect to a diluent storage tank, preferably by jet or fluid connection.

[0027] Preferably, the at least one liquid pump, more preferably any one of the at least one liquid pump is connected to a water tank, and the liquid pump should be separate from the at least one other liquid pump, more preferably any one of the at least one other liquid pump is connected to a culture medium concentrate storage tank.

[0028] Each or any of the at least one other liquid pump can be connected to the same diluent tank or different diluent tanks.

[0029] Preferably, each liquid pump has one inlet, preferably a single and / or unique inlet, and one outlet, preferably a single and / or unique outlet.

[0030] In this manual, unless otherwise stated, "pump" (when used alone) is understood to mean "liquid pump".

[0031] In this specification, unless otherwise stated, "at least one pump" and "at least another pump" can be understood as "at least one of the pumps" and "at least another of the pumps".

[0032] In this specification, unless otherwise stated, "at least one pump" and "at least another pump" can be understood as "at least one of at least two liquid pumps" and "at least another of at least two liquid pumps".

[0033] The diluent storage tank can be a diluent supply, a diluent distribution network, or a diluent storage tank.

[0034] "Diluent supply" can be understood as: a means, device or component for supplying diluent.

[0035] Preferably, the diluent is water. More preferably, distilled water and / or disinfectant water are used as the diluent.

[0036] The diluent may be an aqueous solution, preferably a sterilized aqueous solution, containing one or more components, such as one or more ions and / or one or more molecules, preferably one or more ions and / or molecules not present in the concentrated culture medium solution, or at different concentrations. For example, the ions may be one or more ions or minerals, such as phosphates, sulfur, magnesium, calcium, iron, or one or more trace elements, while the molecules may be glucose, one or more growth factors, and / or one or more antibiotics.

[0037] In this application, "liquid flow" can be understood as: volumetric flow rate or volumetric flow rate density per unit area, expressed in m / s.

[0038] Preferably, in this application, the liquid flow can be replaced or replaced by the liquid mass flow rate (in kg / s) or the liquid volume flow rate (in m³ / s).

[0039] The device of the present invention also includes a control unit for controlling at least two liquid pumps, preferably, the control unit of any one of the liquid pumps in the device of the present invention.

[0040] The control unit is set and / or programmed and / or configured to control each liquid pump separately and independently.

[0041] Preferably, the control unit is set and / or programmed and / or configured to individually and independently turn on and / or stop the operation of each pump.

[0042] Preferably, the control unit is also set and / or programmed and / or configured to calibrate each pump individually and / or sequentially.

[0043] Furthermore, the control unit is set and / or programmed and / or configured to preferentially calibrate each pump during or during one or more initial calibrations, in terms of: -Based on the volume of liquid injected in time units, preferably measured in real time or with a delay by the weighing scale, and / or -Based on or about or for the operating speed or status of each pump.

[0044] In addition, the control unit is set and / or programmed and / or configured to perform a series of dilutions or multiple series of continuous dilutions based on calibration data, preferably following or after the initial calibration, for each pump. At least one series of continuous dilutions is preferably performed.

[0045] Furthermore, the control unit is also set and / or programmed and / or configured to perform a continuous series of dilutions by operating at least one of the pumps connected to the culture medium concentrate tank during operation of the at least one pump connected to the diluent tank.

[0046] Prioritizing the sequential calibration of each pump can be understood as calibrating the pumps one after another, or calibrating one pump at a time.

[0047] Preferably, the culture medium container is placed on the weighing platform, and / or the weighing platform is for and / or suitable for and / or configured to accommodate the culture medium container.

[0048] Furthermore, the series of dilutions can be implemented according to the desired dilution rate.

[0049] Preferably, the weighing scale is configured to weigh: – The mass contained in the weighing platform, and / or The quality of the container, and / or –Total mass of liquid.

[0050] "Total mass of liquid" can be understood as: – The total mass injected into the container or the weighing platform and / or the total mass flowing out of the container or the weighing platform, preferably the total mass weighed after one pump stops, or after each pump or at least two pumps stops, and / or - The mass of liquid injected into or flowing out of the container or the weighing platform, preferably injected or flowing out in real time, and / or the change in the mass of liquid injected into or flowing out of the container or the weighing platform, preferably injected or flowing out in real time, preferably the mass measured after one pump stops or after each pump or at least two pumps stops, that is, the mass of liquid flowing in the container or the weighing platform.

[0051] "Stop" can be understood as the interruption of pump operation, especially after the period of commissioning or operation.

[0052] "Real-time" can be understood as: - Every instant of a single liquid (one-time) flow, or every instant of multiple liquids flowing simultaneously, or This process continues when a single liquid (in one flow) or multiple liquids flow simultaneously, or – This process occurs with the simultaneous flow of a single liquid (one time) or multiple liquids.

[0053] "Real-time weighing" can be understood as: continuously weighing the mass of liquid injected into or flowing out of the container or the weighing platform, or weighing the flow rate of liquid during the flow or outflow process.

[0054] "Delayed weighing" can be understood as: in particular, in contrast to "real-time weighing", weighing the total mass of liquid injected (or flowing in) or flowing out (leaking out) into the container or the weighing platform after the pump stops or after the pump has finished running.

[0055] Preferably, each pump is adapted to or used to connect a flow channel, and preferably connects two different flow channels.

[0056] Preferably, the concentrated culture medium solution and / or the components of the concentrated culture medium solution are a culture medium solution that needs to be diluted.

[0057] Preferably, the concentrated culture medium solution and / or the components of the concentrated culture medium solution are designed, preferably specially designed and / or adapted and / or configured and / or prepared, and preferably specially prepared.

[0058] In other words, preferably, the concentrated culture medium solution and / or the components of the concentrated culture medium solution are designed, preferably specially designed and / or adapted and / or configured and / or prepared, preferably specially prepared, for dilution.

[0059] Preferably, the concentrated culture medium solution is not suitable for adapting, setting up, and designing the culture of microorganisms. In other words, the concentrated culture medium solution cannot be used (directly) as a culture medium, nor can it achieve the optimal growth or culture effect of microorganisms.

[0060] Preferably, each culture medium solution has a given or specific ratio between the different elements in its composition. Therefore, the concentrated culture medium solution can be further concentrated, that is, the components of a given culture medium can be concentrated until the solubility limit of a certain component is reached.

[0061] "Element" can be understood as: an atom, an ion, or a salt, including metal atoms or ions, such as trace elements; a molecule and / or metabolite, such as proteins; and / or growth factors and / or antibiotics.

[0062] The device of the present invention may include a processing unit.

[0063] Preferably, the control unit includes the processing unit.

[0064] Alternatively, the processing units may be independent and / or separate from each other.

[0065] The control unit can be connected to and / or communicate with and / or connect with the processing unit.

[0066] The processing unit can be set and / or programmed and / or configured to calibrate each pump and / or perform one, multiple, or each calculation and / or judgment step.

[0067] Therefore, in this specification, features associated with the control unit are preferably also applied to the processing unit, and vice versa.

[0068] Preferably, each dilution in the same consecutive series of dilutions has the same dilution rate or meets the desired dilution rate.

[0069] Preferably, each dilution in the same consecutive series of dilutions has the same dilution ratio.

[0070] Preferably, the control unit is set and / or programmed and / or configured to implement multiple groups and / or different series of dilutions.

[0071] Preferably, a series of dilutions, or a series of dilutions within the same set, or a given series of dilutions, are intended to prepare the same culture medium solution and / or solutions with the same (desired) dilution ratio.

[0072] Preferably, the control unit is set and / or programmed and / or configured to implement multiple series of dilutions (different or varied).

[0073] Preferably, the control unit is set and / or programmed and / or configured to perform at least two sets of serial dilutions (different or different) to produce at least two different culture medium solutions and / or culture medium solutions with different / different dilution rates (desired), preferably producing multiple different culture medium solutions and / or multiple culture medium solutions with different / different dilution rates (desired).

[0074] Preferably, the control unit is set and / or programmed and / or configured to start and stop the operation of the liquid pump individually and independently.

[0075] Preferably, the control unit is set and / or programmed and / or configured to start and stop the operation of the liquid pumps individually and independently during their respective predetermined operating periods.

[0076] Preferably, the control unit is set and / or programmed and / or configured to start the pump with the shortest runtime during the operation of the other pumps with the longest runtime when performing a continuous series of dilutions.

[0077] Preferably, the pump with the shortest running time is the pump that should be connected to the culture medium concentrate storage tank, and / or the pump with the longest running time is the pump that should be connected to the diluent storage tank.

[0078] The control unit and / or the processing unit may be set and / or programmed and / or configured to calibrate each pump individually and sequentially during initial calibration, with each pump being calibrated only once and / or only once, preferably before each series of dilutions.

[0079] Preferably, the control unit and / or the processing unit can be set and / or programmed and / or configured to: preferably during initial calibration and / or before performing a series of dilutions, preferably before each series of dilutions, calculate and / or determine and / or estimate and / or set the operating and / or working time of each pump, the process preferably based on the liquid mass weighed in real time by the weighing scale and the desired dilution rate and / or target dilution rate.

[0080] Preferably, the calibration data includes runtime and / or operating time, preferably for a given operating state or based on the operating state, and / or flow rate per pump.

[0081] Preferably, a set, multiple sets, or series of dilutions are performed based on runtime and / or operating time, preferably for a given operating state, or according to the operating state, and / or the flow rate of each pump.

[0082] Preferably, the device of the present invention includes a holding device.

[0083] Preferably, the holding device is configured to hold a container.

[0084] Preferably, the container is placed on the weighing platform.

[0085] Preferably, the container is used to collect liquid and / or fluid flow from the pump.

[0086] Preferably, the device of the present invention includes a fixing device.

[0087] Preferably, the fixing device is configured to fix and position one end, i.e. the distal end, of the fluid connection device, primarily above the weighing platform.

[0088] Preferably, the outlet of each liquid pump is connected to the other end, i.e., the proximal end, of each fluid connection device.

[0089] Preferably, the fixing device is configured to fix and position the distal ends of each fluid connection device.

[0090] Preferably, the fixing device is further configured to orient the distal end of the fluid connection device so that the liquid generated by each pump flows toward the weighing platform.

[0091] Preferably, the control unit is further set and / or programmed and / or configured to adjust and / or calibrate the calibration data of one, multiple, or each pump during at least one dilution process in which a series of dilutions are performed.

[0092] Preferably, the control unit is further set and / or programmed and / or configured to adjust and / or correct calibration data of one, multiple, or each pump based on the mass of liquid injected by one, multiple, or each pump during at least one dilution of a continuous series of dilutions, preferably the total mass.

[0093] Preferably, when a deviation is detected and / or determined and / or observed between the injected liquid mass and the expected liquid mass (i.e., the liquid mass that should be injected to achieve the desired dilution rate), especially when the deviation is significant or exceeds a preset threshold, the calibration data is adjusted and / or corrected.

[0094] Preferably, the control unit is further set and / or programmed and / or configured to: preferably during or while adjusting and / or correcting calibration data: – By operating one, multiple, or each pump separately, preferably each of at least two pumps, preferably one after another, at least one dilution of a series of dilutions is performed, and / or – Adjust and / or calibrate the calibration data of one, multiple, or each pump based on the total mass of liquid injected by each pump during at least one dilution of a continuous series of dilutions.

[0095] Preferably, "operating one, multiple, or each pump separately" can be understood as: – Pumps whose calibration data has been adjusted and / or corrected (of which one) should run before other pumps; other pumps may be started simultaneously or sequentially. – Operate the pumps whose calibration data has been adjusted and / or corrected sequentially or one after another (other pumps may be operated simultaneously or sequentially). - Operate each pump sequentially or one after another.

[0096] Preferably, the calibration data is adjusted and / or corrected when a difference is detected and / or determined and / or observed between the total amount of liquid injected individually, separately or continuously by each pump whose calibration data has been adjusted and / or corrected and the expected or desired total amount of liquid (i.e., the total amount of liquid that needs to be injected individually, separately or continuously by each pump whose calibration data has been adjusted and / or corrected to achieve the desired dilution rate), especially when the difference is significant or exceeds a preset or predefined threshold.

[0097] Preferably, one or each of the liquid pumps is a peristaltic pump.

[0098] Preferably, in this specification, the concept or term "per unit time" may be equivalent to, correspond to, and / or replaced by any parameter, particularly parameters related to the operation of the pump, which are time-dependent, time-related, or can be defined by time. As an example not limited to this, a parameter set by time may be the pump's operating state or operating speed.

[0099] For example, in the special case of peristaltic pumps, the term "per unit time" can also be defined as the rotation of the rotor per unit time, the rotational speed (or number of revolutions).

[0100] The present invention also provides a weighing and dilution method for preparing culture media, namely the method of the present invention.

[0101] The method includes a calibration step, namely initial calibration, which is implemented and / or carried out by the control unit of each liquid pump.

[0102] The initial calibration steps are performed and / or implemented individually and sequentially for each pump.

[0103] The calibration procedure is based on the mass of liquid weighed by the weighing scale, and is performed and / or carried out after being poured out per unit time.

[0104] The method also includes a series of successive dilutions, referred to as a series dilution, which can be defined as a dilution step and / or referred to as a dilution step.

[0105] The series of dilutions are implemented and / or achieved through the control unit.

[0106] The series of dilutions were performed and / or implemented based on calibration data.

[0107] For each dilution in a series of dilutions, preferably in groups of series dilutions, the method of the present invention includes operating at least one pump via the control unit during the operation of at least another pump, the at least one pump being in communication with the concentrated culture medium solution, and the at least another pump being in communication with the diluent.

[0108] Preferably, the series of dilutions includes or consists of at least two consecutive dilutions, each having or should have the same dilution ratio.

[0109] Preferably, the method of the present invention may include one or more weighing steps of the liquid injected into the container or the weighing platform.

[0110] Preferably, the weighing step includes or consists of weighing the following: – The total mass injected into the container or the weighing platform and / or the total mass flowing out of the container or the weighing platform, preferably after the pumps have stopped, or after each pump has stopped, or after at least two pumps have stopped, and / or - The real-time injection or outflow of liquid mass into or out of the container or the weighing platform, and / or the real-time change in liquid mass into or out of the container or the weighing platform, preferably after the pump stops, or after each pump stops, or after at least two pumps stops, i.e., during the outflow of liquid or liquid mass from the container or the weighing platform.

[0111] Preferably, the weighing step includes weighing the mass of liquid or liquid composition thereof injected sequentially or simultaneously by one, multiple, or each pump.

[0112] Preferably, the mass of the liquid being weighed and / or injected may include or consist of the following: – The mass of liquid injected by the pump in real time or per unit time, preferably when the pumps are running simultaneously or synchronously. The mass of liquid injected sequentially by each pump, either in real time or per unit time, preferably when the pumps are not synchronized. The total mass of liquid injected by each pump sequentially or one pump after another. – The total mass of liquid injected by the pump, preferably after each pump has run or thereafter.

[0113] Preferably, at least one pump connected to the concentrated culture medium solution is started and / or turned on, preferably during the dilution process, after at least one other pump connected to the diluent is started and / or turned on; and / or at least one pump connected to the concentrated culture medium solution is turned off, preferably during the dilution process, before or simultaneously with the shutdown of at least one other pump connected to the diluent.

[0114] Preferably, the method of the present invention includes multiple series of dilutions.

[0115] Preferably, two sets of serial dilutions (different and / or varied) are performed, and preferably each set of serial dilutions (different and / or varied) is achieved at the same or different desired dilution rates.

[0116] Preferably, at least one of the continuous series of dilutions constitutes an adjustment and / or correction step for the calibration data of one, multiple, or each pump.

[0117] Preferably, the adjustment and / or correction steps of the calibration data are performed during at least one dilution in a continuous series of dilutions, based on the mass of liquid injected by one, multiple, or each pump.

[0118] Preferably, the adjustment and / or correction steps for the calibration data include the following: – To perform or execute at least one dilution in a continuous series of dilutions by operating one, multiple, or each pump separately, preferably one after another, and / or – The calibration data of one, multiple, or each pump are adjusted and / or corrected based on the total mass of liquid injected by one, multiple, or each pump during at least one of the above-described continuous series of dilutions.

[0119] Preferably, calibration of one, multiple, or each pump, or an initial calibration step, and / or adjustment and / or correction steps of the calibration data are performed based on or for the operating status or operating speed of the pump.

[0120] Preferably, the adjustment and / or correction steps of the calibration data change the operation and / or operating state of the pump, and more preferably change the operating state of each pump in the device of the present invention.

[0121] Preferably, the calibration of one, multiple, or each pump, preferably an initial calibration step, includes determining the operating or working time of one, multiple, or each pump.

[0122] Preferably, the adjustment and / or correction steps for the calibration data of one, multiple, or each pump further include adjusting and / or correcting the operating or working time of one, multiple, or each pump.

[0123] Preferably, the concentration of the concentrated culture medium solution is at least five times that of the culture medium concentration.

[0124] Preferably, the concentration of the concentrated culture medium solution should be equal to or higher than twice that of the culture medium, and can be used or applied to the culture or growth of microorganisms, preferably three times, more preferably four times, particularly preferably five times, especially preferably six times, more preferably seven times, more preferably eight times, more preferably nine times, even more preferably ten times, especially preferably eleven times, especially preferably twelve times, preferably thirteen times, more preferably fourteen times, even more preferably fifteen times, and especially preferably equal to or higher than twenty times the culture medium concentration, and can be used or applied to the culture or growth of microorganisms.

[0125] Preferably, the concentration of the concentrated culture medium solution is such that all of them reach the solubility limit of one, more or less of the elements contained in the components of the culture medium solution.

[0126] The present invention also provides a data processing apparatus, including means for setting and / or programming and / or configuring to perform the method of the present invention.

[0127] The present invention also provides a computer program, including instructions, which, when executed by a computer, guide it to perform the method of the present invention.

[0128] The present invention also provides a computer-readable medium including instructions that, when executed by a computer, will guide it to perform the method of the present invention.

[0129] Preferably, the weighing and dilution apparatus for preparing culture media according to the invention, especially the control unit and / or processing unit of the apparatus according to the invention, is suitable, preferably suitable, more preferably designed, and particularly preferably specifically designed to implement the weighing and dilution method for preparing culture media according to the invention. Similarly, any technical feature of the weighing and dilution apparatus for preparing culture media according to the invention, especially the control unit and / or processing unit of the apparatus according to the invention, can be directly adapted to the weighing and dilution method for preparing culture media according to the invention, and vice versa. Attached Figure Description

[0130] The invention will be better understood by reading the following description, which is by way of non-limiting example only and with reference to the accompanying drawings, wherein: – Figure 1 An embodiment is schematically illustrated by top and oblique views of a weighing and dilution apparatus for preparing culture media according to the present invention.

[0131] – Figure 2 This is a front view of an embodiment of the weighing and dilution apparatus for preparing culture medium according to the present invention. – Figure 3A side view of the pump, storage tank and / or water supply device, and weighing platform according to an embodiment of the device of the present invention.

[0132] Of course, the embodiments described below are not limiting. In particular, it is conceivable that variations of the invention may include only a certain feature option described below, which is sufficient to contribute to the technical effect or distinguish the invention from the prior art, thus distinguishing it from the other technical features described. This technical option includes at least one functionally defining feature without structural details, or has only partial structural details, provided that only that partial structural detail is sufficient to contribute to the technical effect or distinguish the invention from the prior art.

[0133] In particular, all the described implementations or variations can be combined with each other unless the technical solution is contrary to the combination.

[0134] Components common to multiple figures in the accompanying drawings and subsequent description retain the same numbering. Detailed Implementation

[0135] Culture media are the primary carriers and are considered consumables, essential for many business areas that are based on or involve microbial culture.

[0136] The preparation of culture media is complex and requires specialized methods, facilities, and equipment. Therefore, commercially available culture media are now sold in individual plastic containers for immediate use; plastic bags or pouches are the most common packaging for culture media.

[0137] Many laboratories, especially those in the fields of agricultural products, pharmaceuticals, and medicine, use tens of liters of culture media every day, whether for research or industrial use (particularly quality control), as well as some public or university laboratories.

[0138] Therefore, the total amount of culture medium delivered regularly is considerable. In addition, the amount of plastic used is also quite substantial.

[0139] In addition, the laboratory requires a large storage space. Furthermore, this means that laboratory personnel will have to perform a significant amount of handling and require suitable storage conditions.

[0140] In the prior art, there is a known document FR3017706B1, which relates to a weight distribution device. However, the weight distribution device described in FR3017706B1 does not solve the problem raised in this application.

[0141] Therefore, referring to Figures 1 to 3The present invention provides a non-limiting embodiment of a weighing and dilution device 1 for preparing culture media, referred to as device 1. By using the weighing and dilution device to prepare culture media through dilution, culture media can be prepared directly in the laboratory on demand, requiring no (or almost no) user intervention.

[0142] The device 1 includes a weighing scale 2, hereinafter referred to as "scale 2".

[0143] Device 1 includes weighing platform 3.

[0144] According to the implementation method, the weighing platform 3 can be detachably placed on the scale 2.

[0145] The scale 2 is set to weigh the mass placed on the weighing platform 3.

[0146] Device 1 is configured (and / or includes means of setting) to correct and / or adjust the tilt (or tilt angle) and / or orientation of the weighing platform relative to the vertical line (or gravity vector).

[0147] According to a non-limiting embodiment, the weighing platform 2 cooperates with the sensing element 21 or weighing element 21 of the scale 1 to transmit the force applied by the weighing platform 2 (the mass contained in or falling on the weighing platform 2) to the sensing element 21. According to a non-limiting embodiment, the sensing element 21 consists of two weighing shafts 21.

[0148] The device 1 includes at least two liquid pumps 41 and 42, hereinafter referred to as "pumps 41 and 42".

[0149] Pumps 41 and 42 are configured to each generate a flow rate and / or volume of liquid that falls onto the weighing platform 3. In other words, pumps 41 and 42 are configured to generate a flow rate and / or volume of liquid that is injected into the weighing platform 3.

[0150] At least one pump 41, 42 will be connected to the culture medium concentrate storage tank 7.

[0151] At least one other pump 41, 42 is connected to diluent storage tank 8. Diluent storage tank 8 is a water supply device 8 according to the embodiment shown.

[0152] According to the present invention, various types of liquid pumps 41, 42 can be used. For example, according to the present invention, one, multiple, or each liquid pump 41, 42 can be: a piston pump, a diaphragm pump, a syringe pump, a gear pump, or a peristaltic pump.

[0153] According to a non-limiting embodiment, device 1 includes two pumps 41 and 42.

[0154] However, the present invention may use more than two pumps 41, 42. For example, device 1 may include up to six pumps 41, 42. For example, device 1 may include four or six pumps 41, 42.

[0155] The apparatus 1 may include four or six pumps 41, 42, and at least one of the four to six pumps 41, 42 is connected to a diluent tank 8. For example, the other three to five pumps 41, 42 may be connected to a culture medium concentrate or a culture medium concentrate tank 7; preferably each pump is connected to a different culture medium concentrate or a different culture medium concentrate tank 7.

[0156] Device 1 includes control units for two pumps 41 and 42.

[0157] The control unit is set and / or programmed and / or configured to control each pump 41, 42 separately and independently.

[0158] Therefore, the control unit is set and / or programmed and / or configured to monitor and / or control, in particular, the start-up (or operation), shutdown (termination of operation), operating status, and operating time of each pump 41, 42.

[0159] The control unit can be configured to control the operation of each pump 41, 42 in a default, selected (selected by the user or control unit, e.g., the required volume or mass of liquid to be pumped) or predefined operating state during operation when a desired or anticipated volume and / or mass of liquid needs to be injected. In this application, "liquid volume" can be understood as the mass and / or volume of liquid.

[0160] The operating state of pumps 41 and 42 can correspond to, be equivalent to, or depend on the mass and / or volume of liquid pumped and / or injected by pumps 41 and 42 per unit time, and / or the flow rate of liquid pumped and / or injected by pumps 41 and 42 per unit time. The operating state is controllable and / or correctable, especially through the control unit.

[0161] In actual operation, the control units of liquid pumps 41 and 42 operate and / or monitor: -Based on the required liquid volume, the operating status of pumps 41 and 42, and the operating time of pumps 41 and 42 based on calibration data, and - The operating status of pumps 41 and 42 based on calibration data.

[0162] Furthermore, the operation of pumps 41 and 42 depends on and / or is subject to calibration data. In other words, correcting and / or changing and / or adjusting and / or revising the calibration data of pumps 41 and 42 will alter the operation of pumps 41 and 42.

[0163] In addition, the calibration data is specific to pumps 41 and 42. Therefore, each pump 41 and 42 in device 1 may have different calibration data.

[0164] Calibration data may include either the flow rate of liquid pumped (and / or injected) per unit time (depending on operating conditions), or the volume and / or mass of liquid pumped (and / or injected) per unit time (depending on operating conditions).

[0165] The calibration data may also include time parameters (or time operating parameters of pumps 41 and 42), such as the displacement of the pumping mechanism of pumps 41 and 42 over time.

[0166] The time parameters of pumps 41 and 42 (or the displacement of the pumping mechanism of pumps 41 and 42 over time) can be the displacement frequency and / or displacement speed and / or displacement distance of one or more pumping mechanisms of pumps 41 and 42.

[0167] For example, a time parameter for pumps 41 and 42 could be: - The longitudinal movement distance and / or speed of the injection rod or piston (for injection pumps and piston pumps). - The pulse or oscillation frequency of the membrane (for membrane pumps), - The rotational speed or frequency of the rollers and / or gears or rotors and / or the number of revolutions (for gear pumps and peristaltic pumps).

[0168] The operating state of the pump can be defined or corresponding to the time parameters of pumps 41 and 42; that is, the displacement frequency and / or displacement speed and / or displacement distance of one or more pumping mechanisms of pumps 41 and 42.

[0169] The timing parameters of pumps 41 and 42 are adjusted, controlled, and / or depend on (e.g., based on linear or polynomial relationships or mathematical functions) the operating state of pumps 41 and 42 and / or the operating duration of pumps 41 and 42 (for the desired liquid volume). In other words, by changing and / or adjusting the timing parameters of pumps 41 and 42, the operating state and / or operating duration of pumps 41 and 42 can be changed and / or controlled.

[0170] The flow rate of liquid pumped (and / or injected) per unit time and the volume and / or mass of liquid pumped (and / or injected) per unit time depend on, are controlled by and / or are based on a function of the pump time parameters 41, 42 (e.g., according to a linear or polynomial relationship or according to a mathematical function).

[0171] Furthermore, the calibration data, runtime, and operating status of pumps 41 and 42 are based on or depend on the type of liquid used, especially its viscosity, density, or surface tension. In addition, changing, replacing, or using different or varying concentrated media solutions will alter the calibration data, runtime, and operating status of pumps 41 and 42.

[0172] The flow rate and / or volume and / or mass of liquid produced, pumped, or injected by the same pump under the same duration and operating conditions are not constant for most liquid pumps. Therefore, for the same pump, under the same conditions and operating parameters, the volume of liquid pumped or injected may differ between two consecutive pumping trips or two consecutive uses. Furthermore, it is frequently observed that the flow rate and / or volume and / or mass of liquid produced, pumped, or injected by the same pump under the same duration and operating conditions will deviate, change, or alter over time.

[0173] However, batches of the same type of culture medium prepared in the same sequential dilution process must have the same composition (or nearly identical composition, with predefined or preset ranges for error in the molecular concentration of chemical elements and / or components). Furthermore, for the same desired dilution rate, especially for each dilution in the same sequential dilution series, the flow rate and / or volume and / or mass of liquid generated, pumped, or injected at each dilution should be substantially the same, preferably consistent.

[0174] To this end, the control unit is also set and / or programmed and / or configured to calibrate each pump 41, 42 separately and sequentially. Each pump 41, 42, considered separately and / or individually, is calibrated based on the mass of liquid injected by the considered pump 41, 42 per unit time. The mass of liquid injected by pumps 41, 42 per unit time is weighed by scale 2.

[0175] Preferably, the scale 2 can weigh the liquid mass in real time or continuously.

[0176] In other words, each pump 41 and 42 is calibrated according to the actual mass of liquid injected by pumps 41 and 42.

[0177] The calibration of pumps 41, 42 prior to dilution, preferably prior to a series of consecutive dilutions, is referred to as or denoted as the initial calibration.

[0178] Preferably, the initial calibration step is performed before dilution, or before each series of dilutions, or before the first series of dilutions (which belongs to the series of dilutions to be performed), or after the device 1 and / or pumps 41, 42 are turned on or started.

[0179] For example, during initial calibration, the control unit is also set and / or programmed and / or configured to control the start of each pump 41, 42 separately and sequentially to inject the desired or predetermined amount of liquid into the weighing station 3.

[0180] Preferably, during the initial calibration, the control unit is also set and / or programmed and / or configured to control the start-up of each pump 41, 42 separately and sequentially, so as to: – Inject the desired or predetermined amount of liquid, and (then) - Based on the actual mass of liquid injected by each pump 41 and 42, calibrate each pump 41 and 42 separately.

[0181] During initial calibration, the control unit (or the processing unit of the control unit, or the processing unit connected to or associated with the control unit) may be set and / or programmed and / or configured to determine and / or calculate calibration data based on the mass of liquid weighed by the scale 2 and injected by the pumps 41, 42 per unit time.

[0182] Preferably, the scale 2 is capable of weighing the liquid volume in real time or continuously, that is, weighing as the liquid flows (i.e., weighing at each response time and / or measurement time of the scale 2).

[0183] The initial calibration step may also include starting pumps 41 and 42 separately and sequentially via the processing unit to inject the desired or predetermined amount of liquid.

[0184] The initial calibration procedure may also include weighing the amount of liquid injected by each pump 41, 42 using scale 2 (valid).

[0185] The preliminary calibration steps may also include steps for determining and / or calculating calibration data, preferably based on the mass of liquid weighed by the scale 2 and injected by the pumps 41 and 42 per unit time.

[0186] To obtain the desired or predetermined amount of liquid injected during initial calibration, the pumps 41 and 42 can be started by the control unit based on calibration data stored and / or recorded by the control unit (or via a dedicated storage unit connected thereto, whether remote or local). The stored calibration data can be previous calibration data (calibration data determined in previous calibrations, or calibration data used during previous dilutions or a series of dilutions), or it can be "default" or original calibration data.

[0187] For example, during initial calibration, the control unit can be configured to control the startup of each pump 41, 42 separately and sequentially during operation when a desired or anticipated volume and / or mass of liquid (which may be default, selected or preset by the user or control unit) is injected into the weighing platform 3 as required or in response. The mass of liquid injected onto the weighing platform 3 is weighed, preferably in real time; in other words, as the liquid flows (i.e., each response time and / or the weighing time of the scale 2), and / or according to the time parameters of the pumps 41, 42, such as the displacement relative to a certain pumping mechanism of the pumps 41, 42 (as described above).

[0188] Additionally, the control unit is set and / or programmed and / or configured to perform a series of continuous dilutions, i.e., by starting pumps 41, 42.

[0189] In addition, the control unit is set and / or programmed and / or configured to start at least one pump 41, 42 of the connected culture medium concentrate storage tank 7 during operation of at least another pump 41, 42 of the connected water supply device 8.

[0190] A series of successive dilutions aims to obtain a set of culture medium batches with identical components. Therefore, the same concentrated culture medium solution stored in tank 7 will be used in the same series of successive dilutions.

[0191] Using pumps 41 and 42 simultaneously during the dilution process can reduce the time required to complete each dilution. Typically, dilution requires operator intervention at each step to perform (by sampling) and / or continuously check the quantity of each type of liquid collected.

[0192] Preferably, particularly during a given dilution or a given calibration period, especially during the initial calibration, the selected, predefined, used or applied operating state can be selected to be different from any one of pumps 41, 42, or the same as all pumps 41, 42.

[0193] Preferably, the operating status of pumps 41 and 42 is reduced, weakened, or reaches a minimum level during calibration, particularly during initial calibration. Using a low level of operating status during calibration can reduce or decrease variability, errors, and / or improve the accuracy of the actual flow rate or effective flow rate, i.e., the flow rate (volume of liquid pumped and / or injected) effectively injected by pumps 41 and 42 from one start-up to the next. Using a low level of operating status during calibration makes the calibration (or the calibration data obtained) more accurate.

[0194] Preferably, during the dilution process, the operating status of pumps 41 and 42 is increased to the highest or maximum. This can reduce the time required to complete each dilution. In addition, the initial calibration can mitigate, limit, or reduce the variability or error of the actual or effective flow rate injected by pumps 41 and 42 during continuous dilution, and / or improve accuracy.

[0195] According to the implementation method, the water supply device 8 can be a water supply network 8. Preferably, the water supply network 8 is a distribution network for distilled water and disinfectant water.

[0196] According to an embodiment, the concentration of the concentrated culture medium solution is equal to or at least five times higher than the concentration of the culture medium obtained after dilution (i.e., the culture medium intended or desired for microbial culture). In other words, the concentration of chemical elements and / or molecules constituting or contained in the concentrated culture medium solution is at least five times the concentration of chemical elements and / or molecules in the culture medium obtained after dilution (i.e., the culture medium intended or desired for microbial culture).

[0197] Therefore, the dilution ratio to be adopted, i.e., the ratio of the volume of the concentrated culture medium solution (delivered by at least one pump 41, 42 connected to the concentrated culture medium solution tank 7) to the volume of water (delivered by at least one pump 41, 42 connected to the water supply device 8), is equivalent to the ratio of the concentration of chemical elements and / or molecules that make up or are contained in the concentrated culture medium solution to the concentration of chemical elements and / or molecules in the culture medium obtained after dilution (i.e., the culture medium expected or anticipated or prepared for microbial culture).

[0198] Pumps 41 and 42 are equipped with and / or include and / or have accessories for use with fluid connection device 9. Fluid connection device 9 may be a pipe, conduit, or pipeline, preferably a flexible pipe.

[0199] The fluid connection device 9 can be provided independently of the device of the present invention. Furthermore, the fluid connection device 9 is a consumable that needs to be replaced periodically.

[0200] However, the calibration data, runtime, and operating status of pumps 41 and 42 depend on or are based on the influence of the fluid connection device 9 used. Furthermore, replacing, altering, or using a different fluid connection device 9 will change the calibration data, runtime, and operating status of pumps 41 and 42.

[0201] Preferably, the control unit is also set and / or programmed and / or configured to adjust and / or calibrate the calibration data of one, multiple, or each pump 41, 42 based on the mass of liquid injected by one, multiple, or each pump 41, 42 in at least one dilution process in a continuous series of dilutions.

[0202] During at least one of the continuous series of dilutions, the adjustment and / or correction of the calibration data of one, several, or each pump 41, 42 is referred to as a control step or control calibration.

[0203] The control steps can constitute a single dilution within a series of consecutive dilutions, or they can be performed during a single dilution within a series of consecutive dilutions.

[0204] Control calibration is performed or achieved based on the mass of liquid injected by pumps 41, 42 during at least one dilution process in a continuous series of dilutions.

[0205] During control calibration, the culture medium obtained at each control calibration is preferably a "valid" culture medium that can be used to culture microorganisms.

[0206] Control calibration can be performed or achieved for one, multiple, or each dilution in a series of dilutions.

[0207] The control calibration procedure may also include a step of weighing the amount of liquid injected (valid) by each pump 41, 42 by the scale 2.

[0208] Control calibration may include comparing the expected mass (total expected mass or mass that should be injected by pumps 41, 42) with the mass (effective) injected during at least one dilution (in other words, during control calibration) (the total amount (effective) injected by said pumps 41, 42, or the mass injected by said pumps per unit time).

[0209] In the control step, the mass of the injected liquid is weighed (valid), which can be the total mass of liquid injected after dilution. In this case, the implementation of the continuous series of dilutions remains unchanged. This case allows for verification of the dilution mass; significant deviations or deviations exceeding the threshold may indicate a deviation in the amount of liquid injected by one, multiple, or each pump 41, 42.

[0210] The weighing of the injected liquid in the control step (effectively) can be a real-time measurement of the injected liquid mass while pumps 41 and 42 are running simultaneously. In this case, the implementation of the continuous series of dilutions remains unchanged. Based on this, the sequence, time order, and / or timing of the simultaneous activation of pumps 41 and 42 during a single dilution process allows estimation of the volume of liquid injected by each pump 41 and 42 over time and / or per unit time. This allows for effective adjustment and / or correction of calibration data without altering the dilution rhythm (the rate of preparation of the culture medium solution).

[0211] Control calibration allows for adjustment and / or correction of calibration data for one, multiple, or each of pumps 41, 42 during the dilution process without affecting the rate of culture medium solution preparation.

[0212] In addition, control calibration can monitor the quality of the dilution performed regularly, frequently, or continuously.

[0213] The control calibration can also adjust and / or correct the calibration data of pumps 41 and 42 during continuous dilution.

[0214] In order to optimally adjust and / or correct the calibration data, the control unit also sets and / or programs and / or configures to perform at least one dilution of a series of dilutions, during which control steps are performed or executed by running one, multiple, or each of the pumps 41, 42, respectively and / or sequentially and / or one after another, whose calibration data is adjusted and / or corrected (or whose calibration data requires adjustment and / or correction), and / or reducing the operating state of each of the pumps 41, 42.

[0215] In other words, pumps 41 and 42, whose calibration data are adjusted and / or corrected (or whose calibration data need to be adjusted and / or corrected), do not operate simultaneously or synchronously as in a dilution operation without controlled calibration, but rather operate separately and / or sequentially and / or one after another.

[0216] In this case, the control calibration steps include: calibrating pumps 41 and 42 (running sequentially) based on the mass of liquid that may be injected per unit time, as weighed by scale 2, with their calibration data adjusted and / or corrected (or whose calibration data requires adjustment and / or correction). In this case, the control calibration steps may be the same as the initial calibration steps.

[0217] When calibration is performed by sequentially (not simultaneously) starting pumps 41 and 42, the dilution rate, or the rate at which the culture medium is generated or acquired (obtained through dilution), will slow down during at least one dilution. Conversely, the calibration data will be optimally adjusted and / or corrected during the dilution process (in the case of successive serial dilutions).

[0218] Control calibration can be performed on one, multiple, or each pump 41, 42 during one, multiple, or each dilution. For example, to avoid limiting the dilution rate (or the rate of culture medium preparation), control calibration can be performed on a single pump 41, 42 at each dilution. For example, to optimize the quality of the obtained culture medium (the most accurate possible concentration of the culture medium component obtained through dilution), control calibration can be performed by multiple or each pump 41, 42 at each dilution. According to the invention, all cases and / or all alternatives and / or all intermediate sequences or all sequences included between the embodiments which are non-limiting examples are also foreseeable: - To perform control calibration on a single pump 41, 42 for two-fold, three-fold, four-fold, or five-fold dilutions, it is preferable to calibrate a different pump each time control calibration is performed; - Each pump 41, 42 is calibrated for control purposes to achieve two-fold, three-fold, four-fold, or five-fold dilutions; - To perform control calibration on multiple pumps 41, 42 for dilution by two, three, four or five times, it is preferable to calibrate different pumps each time control calibration is performed; - Each time dilution is performed, a single pump 41 or 42 is controlled and calibrated, preferably a different pump is calibrated each time the control calibration is performed; - Multiple pumps 41 and 42 are controlled and calibrated each time dilution is performed, preferably different pumps are calibrated each time control calibration is performed; - Each pump 41 and 42 is controlled and calibrated during each dilution.

[0219] As a non-limiting example, in the illustrated embodiment (apparatus 1 includes two pumps 41, 42), it is conceivable to alternately perform control calibration for both pumps 41, 42 (each pump 41, 42 of apparatus 1) at each dilution, denoted as D, and perform one or more control calibrations for one of the pumps 41, 42, denoted as S. Such a sequence can be denoted as: DSSSDSSS… According to an embodiment, the device includes a holding device 5 for holding the container.

[0220] The container should be placed on the weighing platform 3.

[0221] The retaining device 5 extends approximately vertically from the weighing platform 3.

[0222] The container is used to collect liquid from and / or injected by pumps 41, 42 (on the weighing platform 3). The liquid (discharged by pumps 41, 42) is used to be collected and / or stored and / or injected and / or filled into the container. The container can be any container adapted and / or configured to hold culture medium, especially bags or capsules.

[0223] According to one embodiment, the holding device 5 includes two facing vertical walls. The two vertical walls extend vertically from the weighing platform 3.

[0224] According to a non-limiting embodiment, the holding device 5 (especially the vertical wall) and the weighing platform are an integral (or combined, or a unique and identical element).

[0225] The two vertical walls of the retaining device 5 are configured to clamp, accommodate, or guide the container.

[0226] The retaining device 5, preferably a vertical wall, is configured to secure the container in the open position. The retaining device, preferably two vertical walls, includes means for fixing it around the container opening (single opening). Therefore, liquid generated by pumps 41 and 42 is injected into the container.

[0227] The two vertical walls of the retaining device 5 are funnel-shaped on one side of the weighing platform 3. In other words, the distance between the two vertical walls facing each other on one side of the weighing platform 3 is greater than the distance between the two vertical walls on the upper side (or the side of the fixing device 6).

[0228] According to an embodiment, the device 1 includes a fixing device 6, which is configured to fix and position one end 91, i.e., the distal end 91, of the fluid connection device 9 generally above the weighing platform 3.

[0229] The outlet 401 of each liquid pump 41, 42 is configured to connect to the other end 92 of the fluid connection device 9, i.e., the proximal end 92.

[0230] According to an embodiment, the device 1 includes a movable part 10.

[0231] The movable part 10 is configured to have a retracted position and an extended position.

[0232] The movable part 10 is configured to be in a retracted position and an extended position relative to the part 11 (or column 11 or wall 11) of the device 1. The part 11 extends mainly vertically and / or extends vertically relative to the symmetrical platform 3.

[0233] According to the embodiment, the fixing device 6 is installed at one end of the movable part 10 of the device 1, or is partially or included in or constitutes the movable part 10 of the device 1.

[0234] Device 1 (or control unit or processing unit) is configured to deploy component 11 when pumps 41 and 42 are started, so that liquid flows onto weighing platform 3. Device 1 (or control unit or processing unit) is configured to retract component 11 when pumps 41 and 42 are not running (or pumps 41 and 42 have stopped running), so that excess liquid, leakage, or cross-contamination can flow out of weighing platform 3.

[0235] According to the embodiment, pumps 41 and 42 are used to connect to an additional fluid connection device 900.

[0236] The 402 inlet of each pump 41, 42 is used to connect to one end of the additional fluid connection device 900, namely the proximal end 901.

[0237] The culture medium concentrate storage tank 7 and the water supply device 8 are used to connect one end 902 of the additional fluid connection device 900, i.e., the remote end 902.

[0238] According to a non-limiting embodiment, and not limited thereto, one, multiple, or each of the liquid pumps 41, 42 in device 1 are peristaltic pumps.

[0239] Peristaltic pumps are inexpensive and robust. Furthermore, they prevent liquid contamination. In fact, there is no direct contact between the peristaltic pump (especially its working parts) and the pumped liquid; only the connecting parts or connecting devices (hoops or flexible tubing) come into contact with the liquid.

[0240] However, peristaltic pumps have a drawback: they are highly susceptible to variations between pumping operations (or between two consecutive uses or starts of pumps 41 and 42), and / or deviations in liquid flow rate, and / or deviations in liquid volume, and / or deviations in the quality of liquid generated or pumped over time.

[0241] Furthermore, the device 1 according to the invention is particularly suitable for solving the inherent problems of peristaltic pumps.

[0242] When using peristaltic pumps 41 and 42, the fluid connection device 9 and the additional fluid connection device 900 form only one and identical fluid connection devices. In practice, when using a peristaltic pump, the fluid connection device (hose or flexible tube) extends into and through the pump. Specifically, when using a peristaltic pump, the portion of the fluid connection device extending into pumps 41 and 42 is held between the fixed and rigid circular body and the rollers of pumps 41 and 42. Furthermore, when using peristaltic pumps 41 and 42, components 401 and 402 form or constitute channels or inserts for the connection device within the pump.

[0243] Of course, the present invention is not limited to the embodiments described above, and modifications can be made to these embodiments without departing from the scope of the invention.

[0244] In particular, all the described implementations or variations can be combined with each other unless the technical solution is contrary to the combination, and can be combined in the following ways: – The method of the present invention includes and / or the processing unit of the present invention is configured to perform multiple series of dilutions and / or multiple calibrations. The method of the present invention includes and / or the processing unit of the present invention is configured to perform an initial calibration by a series of dilutions, and / or The method of the present invention includes and / or the processing unit of the present invention is configured to perform at least one controlled calibration through a series of dilutions. Two sets of serial dilutions are performed, preferably with different desired dilution rates.

Claims

1. A weighing and dilution apparatus (1) for preparing culture media, characterized in that, The device (1) includes: - Weighing scale (2) and -Chengtai (3); The weighing scale is configured to weigh the mass that falls onto the weighing platform. - At least two liquid pumps (41, 42) are configured to each generate a liquid flow falling onto the weighing platform, wherein at least one pump is connected to a culture medium concentrate tank (7) and at least the other pump is connected to a diluent tank (8). - The control unit for the at least two liquid pumps is set and / or programmed and / or configured to control each pump separately and independently, and is used for: • Each pump is calibrated individually and / or sequentially based on the mass of liquid injected per unit time as measured by the weighing scale, and then • Based on calibration data, a series of continuous dilutions, i.e., serial dilutions, are performed by operating at least one pump connected to the culture medium concentrate tank (7) during the operation of the other pump connected to the diluent tank.

2. The apparatus (1) according to claim 1, characterized in that, Includes a holding device (5), which is configured to hold a container placed on the weighing platform (3); the container is used to receive liquid from the liquid pumps (41, 42).

3. The apparatus according to claim 1 or 2, characterized in that, Includes a fixing device (6) configured to fix and position one end of the fluid connection device, i.e., the far end, generally above the weighing platform (3); the outlet of each liquid pump is connected to the other end of each fluid connection device, i.e., the near end.

4. The apparatus according to any one of claims 1-3, characterized in that, The control unit is also set and / or programmed and / or configured to adjust and / or calibrate the calibration data of one, multiple, or each liquid pump (41, 42) based on the mass of liquid injected by each of the liquid pumps or at least two liquid pumps during at least one dilution process in the continuous series of dilutions.

5. The apparatus according to claim 4, characterized in that, The control unit is also set and / or programmed and / or configured to: - Perform at least one of the series of dilutions by running one, multiple, or each of the liquid pumps (41, 42) separately. - Adjust and / or calibrate the calibration data of one, multiple, or each pump based on the total mass of liquid injected by the pump or each pump during at least one dilution of the continuous series of dilutions.

6. The apparatus according to any one of claims 1 to 5, characterized in that, One or each of the liquid pumps (41, 42) is a peristaltic pump.

7. A weighing and dilution method for preparing a culture medium, characterized in that, include: -The control unit of the weighing dilution device (1) calibrates each of at least two liquid pumps (41, 42) of the weighing dilution device, respectively and sequentially, based on the mass of liquid weighed by the weighing scale (2) of the weighing dilution device and injected per unit time, i.e., initial calibration, and then... - The control unit performs a series of continuous dilutions based on calibration data, i.e., serial dilutions; each dilution in the series includes running at least one pump of the connected medium concentrate solution during the operation of at least another pump of the connected diluent.

8. The method according to claim 7, characterized in that, The operation of at least one pump connected to the concentrated culture medium solution is performed after the start-up of the other pump connected to the diluent; and the shutdown of at least one pump connected to the concentrated culture medium solution is performed before or simultaneously with the shutdown of at least one other pump connected to the diluent.

9. The method according to claim 7 or 8, characterized in that, It includes multiple series of dilutions; two of the series of dilutions have different expected dilution rates.

10. The method according to any one of claims 7 to 9, characterized in that, At least one of the continuous series of dilutions constitutes an adjustment and / or correction step for one, multiple, or each pump (41, 42) based on calibration data of the liquid mass injected during at least one dilution of the continuous series of dilutions by the pump or each of the at least two pumps.

11. The method according to claim 10, characterized in that, The adjustment and / or correction steps for the calibration data include: - Perform at least one of the continuous series dilutions by running one, multiple, or each pump (41, 42) separately. - Adjust and / or correct the calibration data of one, multiple, or each pump based on the total mass of liquid injected by the pump or each pump during at least one of the continuous series of dilutions.

12. The method according to claim 10 or 11, characterized in that, - The adjustment and / or correction steps for the calibration data also include adjusting and / or correcting the operating status or operating speed of one, multiple, or each pump (41, 42), and / or The adjustment and / or correction steps for the calibration data also include adjusting and / or correcting the operating or working time of one, multiple, or each pump.

13. The method according to any one of claims 7 to 12, characterized in that, - Calibration of one, multiple, or each pump (41, 42) also includes determining the operating time of one, multiple, or each pump, and / or - Calibration of one, multiple, or each pump is performed based on or in response to the operating status or operating speed of one, multiple, or each pump.

14. The method according to any one of claims 7 to 13, characterized in that, The concentration of the concentrated culture medium solution is at least five times higher than the concentration of the culture medium.

15. A data processing apparatus, characterized in that, include: Configure and / or program and / or configure to perform the method described in any one of claims 7 to 14.

16. A computer program, characterized in that, The instructions include instructions that, when a computer executes the program, will direct it to perform the method described in any one of claims 7 to 14.

17. A computer-readable medium, characterized in that, The instructions include, when executed by a computer, directing it to perform the method described in any one of claims 7 to 14.

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