A multi-modal SPR resonance angle and intensity synchronous detection device

Abstract

The application provides a multimodal SPR resonance angle and intensity synchronous detection device, and relates to the technical field of SPR detection.The device comprises a detection box, a sealing cover rotatably installed on the top of the detection box, an inner layer fixed in the detection box, a detection assembly arranged on the top of the inner layer, drive assemblies arranged on the bottom of the detection assembly, a limiting assembly arranged on the top of the detection assembly, and an AI vision-recognized lens end installed in the detection box at positions corresponding to the limiting assembly and the drive assemblies.The detection assembly comprises a filling frame, the two ends of the filling frame are fixedly connected with the inner walls of the two ends of the inner layer, a detection sample is placed in the filling frame, the range of the sample is limited by the limiting assembly, the detection assembly and the drive assemblies are moved along the range of the sample, the SPR detection technology is used to detect different positions in the range of the sample, the reflection angle is adjusted to detect the reflection light intensity, and the whole process is monitored by the AI vision-recognized lens end.

Description

Technical Field

[0001] This invention relates to the field of SPR detection technology, specifically to a multimodal SPR resonance angle and intensity synchronous detection device. Background Technology

[0002] Surface plasmon resonance (SPR) is an instrument based on the optical phenomenon of surface plasmon resonance (SPR) for real-time, label-free detection of biomolecular interactions. Its core system includes an optical detection system, a sensor chip, and a microfluidic system, operating by monitoring changes in the refractive index of the sensor surface. This instrument can provide real-time biophysical parameters such as affinity, kinetics, and thermodynamics of intermolecular interactions. It is primarily used in basic research and drug development fields such as drug screening, antibody characterization, and protein-protein interaction studies. The core of SPR detection technology lies in utilizing the resonance between the plasmon waves on the surface of a metal thin film (usually gold or silver) and incident light. When the wavelength or angle of the incident light meets specific conditions, electrons on the metal surface oscillate collectively, forming surface plasmon waves. This resonance phenomenon leads to a significant decrease in the intensity of reflected light. By detecting changes in the intensity of reflected light, changes in the refractive index of the metal thin film surface can be accurately measured, thus reflecting the dynamic process of intermolecular interactions.

[0003] Existing technology allows for adjusting the incident light angle of the SPR to obtain different reflected light intensities. However, since the incident light needs to contact the metal plate and be reflected by the prism, adjusting the incident light angle can easily lead to a failure to contact the metal plate and also affect the receiver's reception of the reflected light. Summary of the Invention

[0004] To address the shortcomings of existing technologies, the present invention aims to provide a multimodal SPR resonance angle and intensity synchronous detection device to solve the problems mentioned in the background art. To achieve the above objectives, the present invention provides the following technical solution: a multimodal SPR resonance angle and intensity synchronous detection device, comprising: a detection box, a sealing cover rotatably mounted on the top of the detection box, an inner layer fixed inside the detection box, a detection component on the top of the inner layer, driving components on both sides of the bottom of the detection component, a limiting component on the top of the detection component, and an AI (Automatic Resonance Component) installed inside the detection box at positions corresponding to the limiting component and the driving component. The visual recognition lens end; the detection component includes a filling frame, the two ends of which are fixedly connected to the inner walls of the inner layer at both ends, and a sensor array is slidably installed inside the filling frame; a thin metal plate is fixed to the bottom of the filling frame, and a prism is fixed to the bottom of the thin metal plate, the two ends of the thin metal plate and the prism are fixedly connected to the inner walls of the inner layer at both ends; the limiting component includes a sliding plate, the top of which is slidably installed, and the end of the sliding plate facing the sensor array has water outlets equidistantly opened, and a one-way valve is installed inside the water outlet; the driving component includes shafts, two of which are rotatably installed in the inner layers on both sides of the bottom of the prism, and rotating seats are slidably sleeved at the same position on the surface of the two shafts, a light-emitting device is rotatably installed inside the rotating seat on one side of the prism, and a receiving device is rotatably installed inside the rotating seat on the other side of the prism.

[0005] Furthermore, a baffle is slidably inserted into one end of the filling frame, and an outlet is opened in the inner layer and the detection box at the position corresponding to the baffle. A collection box is fixed at the bottom of the outer end of the detection box corresponding to the outlet. A cleaning box is fixed to the outer wall of the other end of the detection box, and two hoses are fixed to the top of the cleaning box.

[0006] Furthermore, a vertical frame is fixed to the top of the cleaning tank, and a through-hole block is slidably installed inside the vertical frame. The hose slides through the through-hole block and the detection box, and one end of the hose that enters the detection box is fixedly connected to the back of the slide plate. The hose is connected to the water outlet of the slide plate. Two first springs are fixed to the top of the vertical frame, and the other end of the first spring is fixedly connected to the top of the through-hole block.

[0007] Furthermore, the driving assembly also includes: a moving frame, a moving slot, a slider, and a second spring. The inner layer has two symmetrically formed moving slots on the surfaces at both ends of the prism, and the moving frame is slidably connected inside the moving slots. The slider is slidably connected inside the moving frame. The two ends of the shaft rotate through the slider. A third spring is fixed to the top of the moving frame, and the bottom of the third spring is fixedly connected to the slider. An angle sensor is fixed to the outside of the slider where the shaft passes through, and the angle sensor is connected and installed to the shaft.

[0008] Furthermore, a connecting rod is fixed to the surface of the shaft protruding from the slider. Two connecting rods on the same side of the inner layer are rotatably connected by a rotating shaft. The two connecting rods are rotatably connected at the middle position of the metal sheet and on the central axis of the prism. A bidirectional electric push rod is fixed at the bottom of the inner layer between two moving slots, and the extended end of the bidirectional electric push rod is fixedly connected to the moving frame. A protrusion is fixed to the surface of the shaft, and the rotating seat is slidably engaged with the protrusion.

[0009] Furthermore, the detection assembly also includes: a side frame, a carriage, a bottom frame, and a retaining frame. The two sides of the filling frame are fixed with side frames, and the carriage is slidably installed inside the side frames. The side of the side frame has a side opening, and the carriage slides out through the side opening. The bottom of the carriage is slidably connected to the bottom frame, and a retaining frame is fixed to one end of the bottom frame. The vertical surface of the rotating seat is fixed with a vertical plate, and the retaining frame is slidably engaged with the surface of the vertical plate. Sealing plates are fixed on both sides of the top of the side frame of the filling frame, and the sealing plates have movement notches corresponding to the movement paths of the carriage and the vertical plate.

[0010] Furthermore, vertical blocks are fixed at both ends of the sensor array, the bottom of the vertical blocks are fixedly connected to the slide, and the vertical blocks slide along the top of the side frame. A movable cavity is opened inside the vertical blocks, and a movable plate is slidably connected inside the movable cavity; wherein, a second spring is fixed between the movable plate and the inner wall of the movable cavity.

[0011] Furthermore, one end of the movable plate facing the baffle is rotatably connected to a rotating rod via a rotating shaft, and the other end of the rotating rod is rotatably connected to an insert plate. The insert plate is housed inside the movable cavity, and a slot is provided on the top of the baffle corresponding to the position of the insert plate. The movable plate pushes the insert plate out of the movable cavity, and after the insert plate is inserted into the slot, it pushes the baffle upward.

[0012] Furthermore, the limiting component also includes: a screw, a connecting plate, and a sliding rod. The connecting plate is fixed to the top of the sliding plate. The screw is rotatably mounted on one end of the inner layer located on the connecting plate, and the connecting plate is threaded onto the surface of the screw. The sliding rod is fixed to the other end of the inner layer located on the connecting plate, and the connecting plate is slidably mounted on the surface of the sliding rod. A drive motor is fixed at the position of the screw corresponding to the interlayer between the inner layer and the detection box, and the output end of the drive motor is fixedly connected to the screw.

[0013] Furthermore, one-way electric push rods are fixed at the bottom of both ends of the connecting plate, and the one-way electric push rods are fixedly connected to the vertical block. A top column is fixed at the position of the connecting plate corresponding to the moving cavity, and an insertion hole is opened at the position of the vertical block corresponding to the top column. The inner layer and the detection box are provided with moving holes for the one-way electric push rods to slide through.

[0014] The beneficial effects of this invention are: This invention allows the sample liquid to be discharged from the outlet and collected in the collection box by the movement of the slide plate and the opening of the baffle. The inside of the filling frame is rinsed by the water pump and hose in the cleaning box through the water outlet of the slide plate, keeping the inside of the filling frame clean and free of residue. The hose can be limited by the through block on the vertical frame and the first spring, so that the hose can be retracted and released in conjunction with the movement of the slide plate.

[0015] Because the angles of the light-emitting device and the receiving device are symmetrical about the central axis of the prism, the reflection angle can be obtained. Furthermore, through the connection of two connecting rods, when the light-emitting device and the receiving device rotate, the slider slides up and down along the moving frame, and the moving frame slides left and right along the moving groove, adjusting the lateral and longitudinal positions of the shaft. This ensures that the angle between the light emitted by the light-emitting device and the receiving device is always in contact with the metal plate. The protrusion on the shaft can engage with the rotating seat, and the light-emitting device and the receiving device can move along the shaft to detect any position within the filling frame.

[0016] The sensor array of the present invention is connected to the slide via a vertical block and can slide along the side frame. The slide is engaged with the vertical plate by sliding along the bottom frame and by a locking frame. Thus, when the sensor array moves, the light-emitting device and the receiving device slide along the shaft, keeping the sensor array, the light-emitting device, and the receiving device always on the same vertical plane. When the angle of the light-emitting device and the receiving device is adjusted by rotating the shaft, the slide slides along the bottom frame, and the locking frame slides along the vertical plate, which can cooperate with the sliding frame to slide along the sliding groove. The slider slides inside the sliding frame. When the light-emitting device and the receiving device move along the X and Y axes, they maintain the connection with the slide. Thus, the movement of the sensor array can drive the light-emitting device and the receiving device to slide along the shaft.

[0017] This invention uses a drive motor to rotate a screw, and a connecting plate that is threadedly engaged with the screw slides along the axis of the slide bar. The slide plate moves inside the filling frame, allowing for the detection of the required liquid range. Simultaneously, the vertical block slides along the side frame, and the sensor array moves synchronously. Through a one-way electric push rod fixedly connected to the vertical block, the sensor array can be moved independently along the area between the slide plate and the baffle. This, in conjunction with the movement of the bottom light-emitting device and the receiving device, enables thorough detection of the liquid within the range.

[0018] When the detection is completed, the present invention first retracts the sensor to the vicinity of the slide plate by a one-way electric push rod. At this time, the top post on the connecting plate passes through the insertion hole and squeezes the moving plate, causing the moving plate to slide along the moving cavity and push out the insertion plate. As the slide plate moves towards the baffle, the insertion plate will eventually be inserted into the slot. The baffle will be slid open upward by the rotation of the rotating rod, exposing the discharge port and facilitating the discharge of the internal detection liquid. Attached Figure Description

[0019] Figure 1This is a schematic diagram of the overall structure of a multimodal SPR resonance angle and intensity synchronous detection device according to the present invention; Figure 2 This is a schematic diagram of the outlet structure of the detection box of the multimodal SPR resonance angle and intensity synchronous detection device of the present invention; Figure 3 This is a schematic diagram of the internal structure of the multimodal SPR resonance angle and intensity synchronous detection device of the present invention; Figure 4 This is a schematic diagram of the defined component structure of a multimodal SPR resonance angle and intensity synchronous detection device according to the present invention; Figure 5 This is a schematic diagram of the drive component structure of a multimodal SPR resonance angle and intensity synchronous detection device according to the present invention; Figure 6 This is a schematic diagram showing the positional relationship between the light-emitting device, the receiving device, and the prism in a multimodal SPR resonance angle and intensity synchronous detection device according to the present invention. Figure 7 This is a schematic diagram of the connection between the rotating seat and the shaft of a multimodal SPR resonance angle and intensity synchronous detection device according to the present invention; Figure 8 This is a schematic diagram showing the connection between the detection component and the vertical plate of a multimodal SPR resonance angle and intensity synchronous detection device according to the present invention; Figure 9 This is a schematic diagram of the connection between the slide and the base frame of a multimodal SPR resonance angle and intensity synchronous detection device according to the present invention; Figure 10 This is a schematic diagram of the surface structure of the connecting plate and sliding plate of the multimodal SPR resonance angle and intensity synchronous detection device of the present invention; Figure 11 This is a schematic diagram of the internal structure of the vertical block of a multimodal SPR resonance angle and intensity synchronous detection device according to the present invention.

[0020] In the diagram: 1. Detection box; 11. Sealing cover; 12. Inner layer; 121. Sealing plate; 122. Moving notch; 13. Collection box; 14. Cleaning box; 141. Vertical frame; 142. Through block; 143. First spring; 144. Hose; 15. Discharge port; 2. Detection assembly; 21. Filler frame; 22. Baffle; 23. Side frame; 24. Side opening; 25. Carriage; 26. Base frame; 27. Sensor row; 28. Vertical block; 29. ​​Slot; 210. Moving slot; 211. Insertion hole; 212. Second spring; 213. Moving plate; 214. Rotating rod; 215. Insertion plate; 216. Clip frame; 3. Limiting components; 31. Slide plate; 32. Screw; 33. Connecting plate; 34. Slide rod; 35. Drive motor; 36. One-way electric push rod; 37. Top column; 38. Water outlet; 4. Drive assembly; 41. Bidirectional electric push rod; 42. Moving frame; 43. Moving cavity; 44. Slider; 45. Third spring; 46. Angle sensor; 47. Connecting rod; 48. Shaft; 49. Light-emitting device; 410. Receiving device; 411. Rotating seat; 412. Protruding strip; 413. Vertical plate; 5. Prism; 6. Thin metal sheets. Detailed Implementation

[0021] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0022] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 6 , Figure 7 and Figure 8As shown in the figure, this disclosure proposes a multimodal SPR resonance angle and intensity synchronous detection device, including a detection box 1, a sealing cover 11 rotatably mounted on the top of the detection box 1, an inner layer 12 fixed inside the detection box 1, a detection component 2 on the top of the inner layer 12, driving components 4 on both sides of the bottom of the detection component 2, a limiting component 3 on the top of the detection component 2, and an AI installed inside the detection box 1 at positions corresponding to the limiting component 3 and the driving component 4. The visual recognition lens end; the detection component 2 includes a filling frame 21, the two ends of which are fixedly connected to the inner walls of the inner layer 12 at both ends, and a sensor array 27 is slidably installed inside the filling frame 21; a metal plate 6 is fixedly fixed to the bottom of the filling frame 21, and a triangular prism 5 is fixedly fixed to the bottom of the metal plate 6, and the two ends of the metal plate 6 and the triangular prism 5 are fixedly connected to the inner walls of the inner layer 12 at both ends; the limiting component 3 includes a sliding plate 31, the top of the filling frame 21 is slidably installed on the sliding plate 31, and the end of the sliding plate 31 facing the sensor array 27 has water outlets 38 equidistantly opened, and a one-way valve is installed inside the water outlets 38; the driving component 4 includes a shaft 48, the shaft 4 The device has two shafts 48, which are rotatably installed in the inner layers 12 on both sides of the bottom of the prism 5. Rotating seats 411 are slidably sleeved at the same position on the surface of the two shafts 48. A light-emitting device 49 is rotatably installed inside the rotating seat 411 on one side of the prism 5, and a receiving device 410 is rotatably installed inside the rotating seat 411 on the other side of the prism 5. When using the device, the sealing cover 11 is opened, the test sample is placed inside the filling frame 21, the sample range is limited by the limiting component 3, and the detection component 2 and the driving component 4 move along the sample range. The SPR detection technology is used to detect different positions of the sample range, and the reflection angle is adjusted to detect the intensity of the reflected light. The entire process is monitored by the lens end of the AI ​​vision recognition, and the adjusted angle can also be recorded. This device is used for new material testing, measurement, related standardization, certification and accreditation services.

[0023] like Figure 2 , Figure 3 and Figure 4As shown, in some embodiments, a baffle 22 is slidably inserted into one end of the filling frame 21, and an outlet 15 is opened on the inner layer 12 and the detection box 1 at the position corresponding to the baffle 22. A collection box 13 is fixed to the bottom of the outer end of the detection box 1 corresponding to the outlet 15. A cleaning box 14 is fixed to the outer wall of the other end of the detection box 1. Two hoses 144 are fixed to the top of the cleaning box 14. A vertical frame 141 is fixed to the top of the cleaning box 14, and a through block 142 is slidably installed inside the vertical frame 141. The hose 144 slides through the through block 142 and the detection box 1. One end of the hose 144 that enters the detection box 1 is fixedly connected to the back of the slide plate 31. The hose 144 communicates with the water outlet 38 of the slide plate 31. Two first springs 143 are fixed to the top of the vertical frame 141, and the other end of the first spring 143 is fixedly connected to the top of the through block 142.

[0024] Understandably, by moving the slide plate 31 and opening the baffle 22, the sample liquid being tested can be discharged from the outlet 15 and collected by the collection box 13. The water pump and hose 144 in the cleaning box 14 can rinse the inside of the filling frame 21 from the outlet 38 of the slide plate 31, keeping the inside of the filling frame 21 clean and free of residue. The hose 144 can be limited by the through block 142 on the vertical frame 141 and the first spring 143, so that the hose 144 can be retracted and released in conjunction with the movement of the slide plate 31.

[0025] like Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7 As shown, in some embodiments, the driving assembly 4 further includes: a moving frame 42, a moving groove 210, a slider 44, and a second spring 212. Two moving grooves 210 are symmetrically formed on the surfaces at both ends of the inner layer 12, and the moving frame 42 is slidably connected inside the moving grooves 210. The slider 44 is slidably connected inside the moving frame 42. Both ends of the shaft 48 rotate through the slider 44. A third spring 45 is fixed to the top of the moving frame 42, and the bottom of the third spring 45 is fixedly connected to the slider 44. An angle sensor 4 is fixed to the outside of the slider 44 where the shaft 48 extends. 6. Angle sensor 46 is connected and installed with shaft 48. A connecting rod 47 is fixed on the surface of shaft 48 that extends out of slider 44. Two connecting rods 47 on the same side of inner layer 12 are rotatably connected by a rotating shaft. The position where the two connecting rods 47 are rotatably connected is located in the middle of metal plate 6 and on the central axis of prism 5. A bidirectional electric push rod 41 is fixed at the bottom of inner layer 12 between two moving slots 210, and the extended end of bidirectional electric push rod 41 is fixedly connected to moving frame 42. A protrusion 412 is fixed on the surface of shaft 48, and rotating seat 411 is slidably engaged with protrusion 412.

[0026] It is understandable that a motor is installed on one of the shafts 48 in the inner layer 12 to drive the shaft 48 to rotate, and the angle sensor 46 detects the changed angle value. Since the angle between the light-emitting device 49 and the receiving device 410 is symmetrical about the central axis of the triangular prism 5, the reflection angle can be obtained. Through the connection of the two connecting rods 47, when the light-emitting device 49 and the receiving device 410 rotate, the slider 44 slides up and down along the moving frame 42, and the moving frame 42 slides left and right along the moving groove 210, adjusting the lateral and longitudinal positions of the shaft 48, thereby keeping the angle between the light emitted by the light-emitting device 49 and the receiving device 410 always in contact with the metal plate. The protrusion 412 on the shaft 48 can be engaged with the rotating seat 411, and the light-emitting device 49 and the receiving device 410 move along the shaft 48 to detect any position within the filling frame 21. The light-emitting device 49 and the receiving device 410 can be understood as follows: the light-emitting device 49 includes a light source that emits broadband light. The receiver 410 includes a polarizer, a waveplate, and an analyzer and a spectrometer. The sensor array 27 is a PR sensing module. Its principle is as follows: the broadband light generated by the light source is polarized by the polarizer to obtain polarized light. The waveplate introduces an additional phase difference between the P-polarized light and the S-polarized light in the polarized light to obtain polarized interference light. The polarized interference light is reflected by the SPR sensing model on which the sample to be tested is placed to obtain polarized interference light with a phase change. The interference spectrum is obtained by the spectrometer. The SPR phase change corresponding to samples with different refractive indices is obtained according to the interference spectrum, so as to detect the sample to be tested. The reflection intensity is detected by the change of the reflection angle in conjunction with the sensor array 27. The reflected light intensity can be used as an auxiliary signal, such as an intensity-type SPR sensor, when monitoring at a fixed incident angle. The angle position and the corresponding intensity distribution are obtained simultaneously to improve the signal-to-noise ratio or correct drift. The technical principle of this part is similar to that used in patent CN110927122B.

[0027] like Figure 7 , Figure 8 and Figure 9As shown, in some embodiments, the detection component 2 further includes: a side frame 23, a slide 25, a bottom frame 26, and a locking frame 216. The side frames 23 are fixed to both sides of the filling frame 21. The slide 25 is slidably installed inside the side frames 23. A side opening 24 is provided on the side of the side frame 23, and the slide 25 slides through the side opening 24. The bottom of the slide 25 is slidably connected to the bottom of the bottom frame 26, and a locking frame 216 is fixed to one end of the bottom frame 26. A vertical plate 413 is fixed to the vertical surface of the rotating seat 411, and the locking frame 216 is slidably engaged with the vertical plate 413. 3. On the surface, sealing plates 121 are fixed on both sides of the top of the side frame 23 of the filling frame 21. The sealing plates 121 have moving notches 122 corresponding to the moving paths of the slide 25 and the vertical plate 413. Vertical blocks 28 are fixed at both ends of the sensor row 27. The bottom of the vertical blocks 28 is fixedly connected to the slide 25, and the vertical blocks 28 slide along the top of the side frame 23. A moving cavity 43 is opened inside the vertical blocks 28, and a moving plate 213 is slidably connected inside the moving cavity 43. A second spring 212 is fixed between the moving plate 213 and the inner wall of the moving cavity 43.

[0028] It should be noted that the sensor row 27 is connected to the slide 25 via the vertical block 28 and can slide along the side frame 23. The slide 25 slides along the bottom frame 26 and is engaged with the vertical plate 413 via the sliding frame 216. Thus, when the sensor row 27 moves, the light-emitting device 49 and the receiving device 410 slide along the shaft 48, keeping the sensor row 27, the light-emitting device 49, and the receiving device 410 always on the same vertical plane. When the angle of the light-emitting device 49 and the receiving device 410 is adjusted by rotating the shaft 48, the slide 25 slides along the bottom frame 26, and the frame 216 slides along the vertical plate 413 to cooperate with the moving frame 42 to slide along the moving groove 210. The slider 44 slides inside the moving frame 42. When the light-emitting device 49 and the receiving device 410 move along the X and Y axes, they maintain the connection with the slide 25. Thus, the movement of the sensor row 27 can drive the light-emitting device 49 and the receiving device 410 to slide along the shaft 48.

[0029] like Figure 4 , Figure 8 , Figure 9 , Figure 10 and Figure 11As shown, in some embodiments, the movable plate 213 is rotatably connected to a rotating rod 214 at one end facing the baffle 22 via a rotating shaft, and the other end of the rotating rod 214 is rotatably connected to an insert plate 215. The insert plate 215 is housed inside the movable cavity 43, and a slot 29 is provided on the top of the baffle 22 corresponding to the position of the insert plate 215. The movable plate 213 pushes the insert plate 215 out of the movable cavity 43, and after the insert plate 215 is inserted into the slot 29, it pushes the baffle 22 upward. The limiting component 3 also includes a screw 32, a connecting plate 33, and a sliding rod 34. The top of the sliding plate 31 is fixed with the connecting plate 33, and the inner layer 12 is rotatably mounted with the screw 32 at one end of the connecting plate 33, and the connecting plate 33 is threaded. The inner layer 12 is fitted onto the surface of the screw 32. A slide rod 34 is fixed at the other end of the connecting plate 33, and the connecting plate 33 is slidably fitted onto the surface of the slide rod 34. A drive motor 35 is fixed at the position of the inner layer 12 and the interlayer of the detection box 1 corresponding to the screw 32, and the output end of the drive motor 35 is fixedly connected to the screw 32. One-way electric push rods 36 are fixed at the bottom of both ends of the connecting plate 33, and the one-way electric push rods 36 are fixedly connected to the vertical block 28. A top post 37 is fixed at the position of the connecting plate 33 corresponding to the moving cavity 43. An insertion hole 211 is opened at the position of the vertical block 28 corresponding to the top post 37. The inner layer 12 and the detection box 1 have a moving hole through which the one-way electric push rod 36 slides.

[0030] It should be noted that the drive motor 35 drives the screw 32 to rotate, the connecting plate 33 and the screw 32 are threaded together and slide along the slide bar 34 axially, the slide plate 31 moves along the inside of the filling frame 21, which can target the liquid range to be detected. At the same time, the vertical block 28 slides along the side frame 23, and the sensor row 27 moves synchronously. Through the fixed connection between the one-way electric push rod 36 and the vertical block 28, the sensor row 27 can be moved alone along the area between the slide plate 31 and the baffle 22 by the one-way electric push rod 36, thereby cooperating with the movement of the bottom light-emitting device 49 and the receiving device 410. The device moves to fully detect the liquid within its range. Upon completion of the detection, the sensor array 27 is first retracted to the vicinity of the slide plate 31 by the one-way electric push rod 36. At this time, the top post 37 on the connecting plate 33 passes through the insertion hole 211 and presses against the moving plate 213, causing the moving plate 213 to slide along the moving cavity 43 and push out the insertion plate 215. As the slide plate 31 moves toward the baffle 22, the insertion plate 215 will eventually be inserted into the slot 29. The baffle 22 will slide upward and open by the rotation of the rotating rod 214, exposing the outlet 15 to facilitate the discharge of the detected liquid.

[0031] Working principle: When using the device, open the sealing cover 11, place the test sample inside the filling frame 21, limit the sample range by the limiting component 3, and move the detection component 2 and the driving component 4 along the sample range. Using SPR detection technology, different positions within the sample range are detected. Simultaneously, the reflection angle is adjusted to detect the intensity of reflected light. The sensor array 27 is connected to the slide 25 via the vertical block 28 and can slide along the side frame 23. The slide 25 slides along the bottom frame 26 and is engaged with the vertical plate 413 by sliding along the locking frame 216. Furthermore, when the sensor row 27 moves, the light-emitting device 49 and the receiving device 410 slide along the shaft 48, keeping the sensor row 27, the light-emitting device 49, and the receiving device 410 always on the same vertical plane. When the angle of the light-emitting device 49 and the receiving device 410 is adjusted by rotating the shaft 48, the slide 25 slides along the bottom frame 26, the clamping frame 216 slides along the vertical plate 413 to cooperate with the sliding frame 42 sliding along the sliding groove 210, and the slider 44 slides inside the sliding frame 42. This ensures that the light-emitting device 49 and the receiving device 410 remain on the same vertical plane. When device 410 moves along the X and Y axes, it maintains its connection with the slide 25. The movement of sensor row 27 then drives the light-emitting device 49 and receiving device 410 to slide along shaft 48. A motor is installed in the inner layer 12 corresponding to one of the shafts 48, driving the shaft 48 to rotate. The changed angle value is detected by angle sensor 46. Because the angles of the light-emitting device 49 and receiving device 410 are symmetrical about the central axis of the prism 5, the reflection angle can be calculated. Furthermore, the two connecting rods 47... The connection allows the light-emitting device 49 and the receiving device 410 to rotate, with the slider 44 sliding up and down along the moving frame 42, and the moving frame 42 sliding left and right along the moving groove 210. This adjusts the lateral and longitudinal positions of the shaft 48, ensuring that the angle between the light emitted by the light-emitting device 49 and the receiving device 410 is always in contact with the metal plate. The protrusion 412 on the shaft 48 can engage with the rotating seat 411, and the light-emitting device 49 and the receiving device 410 can move along the shaft 48 to detect any position within the filling frame 21, using AI. The visual recognition lens monitors the entire process. A drive motor 35 rotates the screw 32, and the connecting plate 33, threadedly engaged with the screw 32, slides axially along the slide bar 34. The slide plate 31 moves inside the filling frame 21, targeting the liquid range to be detected. Simultaneously, the vertical block 28 slides along the side frame 23, and the sensor array 27 moves synchronously. A one-way electric push rod 36, fixedly connected to the vertical block 28, allows the sensor array 27 to move independently along the area between the slide plate 31 and the baffle 22. This, in conjunction with the movement of the bottom light-emitting device 49 and the receiving device 410, ensures thorough detection of the liquid within the range. Upon completion of detection, the one-way electric push rod 36 retracts the sensor array 27 to the vicinity of the slide plate 31. At this time, the top post 37 on the connecting plate 33 passes through the insertion hole 211 and presses against the moving plate 213, causing the moving plate 213 to slide along the moving cavity 43, pushing out the insertion plate 215. As the slide plate 31 moves towards the baffle 22...The insert plate 215 is eventually inserted into the slot 29, and the rotation of the rotating rod 214 slides the baffle 22 upwards, exposing the outlet 15 to facilitate the discharge of the internal test liquid. Through the movement of the slide plate 31 and the opening of the baffle 22, the test sample liquid is discharged from the outlet 15 and collected by the collection box 13. The water pump and hose 144 in the cleaning box 14 rinse the inside of the filling frame 21 from the outlet 38 of the slide plate 31, keeping the inside of the filling frame 21 clean and residue-free. The through-hole block 142 on the vertical frame 141 and the first spring 143 limit the hose 144, thus coordinating with the movement of the slide plate 31 to retract and extend the hose 144.

[0032] The foregoing has shown and described the basic principles and main features of the present invention and its advantages. It will be apparent to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention.

[0033] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A multimodal SPR resonance angle and intensity synchronous detection device, characterized in that: include, The detection box (1) has a sealing cover (11) rotatably installed on the top of the detection box (1), an inner layer (12) is fixed inside the detection box (1), a detection component (2) is provided on the top of the inner layer (12), a driving component (4) is provided on both sides of the bottom of the detection component (2), a limiting component (3) is provided on the top of the detection component (2), and an AI visual recognition lens is installed inside the detection box (1) at the position corresponding to the limiting component (3) and the driving component (4); The detection component (2) includes a filling frame (21), the two ends of which are fixedly connected to the inner walls of the inner layer (12), and a sensor row (27) is slidably installed inside the filling frame (21). The bottom of the filling frame (21) is fixed with a metal plate (6), and the bottom of the metal plate (6) is fixed with a prism (5). The two ends of the metal plate (6) and the prism (5) are fixedly connected to the inner walls of the two ends of the inner layer (12). The limiting component (3) includes a slide plate (31), the top of the filling frame (21) is slidably mounted with the slide plate (31), the slide plate (31) is provided with water outlets (38) at equal intervals at one end facing the sensor row (27), and a one-way valve is installed inside the water outlet (38); The drive assembly (4) includes two shafts (48), which are rotatably mounted in the inner layers (12) on both sides of the bottom of the prism (5). Rotating seats (411) are slidably sleeved at the same position on the surface of the two shafts (48). A light-emitting device (49) is rotatably mounted inside the rotating seat (411) on one side of the prism (5), and a receiving device (410) is rotatably mounted inside the rotating seat (411) on the other side of the prism (5).

2. The multimodal SPR resonance angle and intensity synchronous detection device according to claim 1, characterized in that: A baffle (22) is slidably inserted into one end of the filling frame (21). The inner layer (12) and the detection box (1) are provided with an outlet (15) at the position corresponding to the baffle (22). A collection box (13) is fixed at the bottom of the outer end of the detection box (1) corresponding to the outlet (15). Among them, a cleaning box (14) is fixed to the outer wall of the other end of the detection box (1), and two hoses (144) are fixed to the top of the cleaning box (14).

3. The multimodal SPR resonance angle and intensity synchronous detection device according to claim 2, characterized in that: The top of the cleaning tank (14) is fixed with a vertical frame (141), and a through block (142) is slidably installed inside the vertical frame (141). The hose (144) slides through the through block (142) and the detection box (1). One end of the hose (144) that enters the detection box (1) is fixedly connected to the back of the slide plate (31). The hose (144) is connected to the water outlet (38) of the slide plate (31). Two first springs (143) are fixed to the top of the vertical frame (141), and the other end of the first spring (143) is fixedly connected to the top of the through block (142).

4. The multimodal SPR resonance angle and intensity synchronous detection device according to claim 1, characterized in that: The driving component (4) also includes: The inner layer (12) has two symmetrically opened moving slots (210) on the surfaces at both ends of the prism (5), and the moving frame (42) is slidably connected inside the moving slots (210). The slider (44) is slidably connected inside the moving frame (42). The two ends of the shaft (48) rotate through the slider (44). The top of the moving frame (42) is fixed with a third spring (45), and the bottom of the third spring (45) is fixedly connected to the slider (44). The slider (44) is fixed with an angle sensor (46) on the outside of the shaft (48) and the angle sensor (46) is connected to the shaft (48).

5. The multimodal SPR resonance angle and intensity synchronous detection device according to claim 4, characterized in that: The shaft (48) passes through the surface of the slider (44) and a connecting rod (47) is fixed thereon. The two connecting rods (47) on the same side of the inner layer (12) are rotatably connected by a rotating shaft. The two connecting rods (47) are rotatably connected at the middle position of the metal plate (6) and the central axis of the prism (5). The bottom of the inner layer (12) is fixed between the two moving slots (210) with a bidirectional electric push rod (41), and the extended end of the bidirectional electric push rod (41) is fixedly connected to the moving frame (42). The shaft (48) has a protrusion (412) fixed on its surface, and the rotating seat (411) is slidably engaged with the protrusion (412).

6. The multimodal SPR resonance angle and intensity synchronous detection device according to claim 5, characterized in that: The detection component (2) further includes: Side frame (23), carriage (25), bottom frame (26), and clip frame (216). The two sides of the filling frame (21) are fixed with side frame (23). The carriage (25) is slidably installed inside the side frame (23). The side of the side frame (23) is provided with a side opening (24), and the carriage (25) slides out from the side opening (24). The bottom of the carriage (25) is slidably connected to the bottom frame (26), and a clip frame (216) is fixed at one end of the bottom frame (26). The vertical surface of the rotating seat (411) is fixed with a vertical plate (413), and the frame (216) is slidably engaged on the surface of the vertical plate (413). The filling frame (21) is fixed with sealing plates (121) on both sides of the top of the side frame (23). The sealing plate (121) has a moving notch (122) corresponding to the moving path of the slide (25) and the vertical plate (413).

7. The multimodal SPR resonance angle and intensity synchronous detection device according to claim 6, characterized in that: The sensor row (27) has vertical blocks (28) fixed at both ends. The bottom of the vertical blocks (28) is fixedly connected to the slide (25), and the vertical blocks (28) slide along the top of the side frame (23). The vertical blocks (28) have a moving cavity (43) inside, and a moving plate (213) is slidably connected inside the moving cavity (43). A second spring (212) is fixed between the movable plate (213) and the inner wall of the movable cavity (43).

8. The multimodal SPR resonance angle and intensity synchronous detection device according to claim 7, characterized in that: The movable plate (213) is rotatably connected to a rotating rod (214) at one end facing the baffle (22) via a rotating shaft, and the other end of the rotating rod (214) is rotatably connected to an insert plate (215). The insert plate (215) is housed inside the movable cavity (43), and a slot (29) is provided on the top of the baffle (22) corresponding to the position of the insert plate (215). The movable plate (213) pushes the insert plate (215) out of the movable cavity (43), and the insert plate (215) pushes the baffle (22) upward after being inserted into the slot (29).

9. The multimodal SPR resonance angle and intensity synchronous detection device according to claim 8, characterized in that: The limiting component (3) also includes: The slide plate (31) is fixed with a connecting plate (33) at the top. The inner layer (12) is rotatably mounted with a screw (32) at one end of the connecting plate (33), and the connecting plate (33) is threaded onto the surface of the screw (32). The inner layer (12) is fixed with a slide (34) at the other end of the connecting plate (33), and the connecting plate (33) is slidably mounted onto the surface of the slide (34). Among them, a drive motor (35) is fixed at the position of the screw (32) corresponding to the interlayer between the inner layer (12) and the detection box (1), and the output end of the drive motor (35) is fixedly connected to the screw (32).

10. The multimodal SPR resonance angle and intensity synchronous detection device according to claim 9, characterized in that: One-way electric push rods (36) are fixed at the bottom of both ends of the connecting plate (33), and the one-way electric push rods (36) are fixedly connected to the vertical block (28). A top column (37) is fixed at the position of the connecting plate (33) corresponding to the moving cavity (43), and an insertion hole (211) is opened at the position of the vertical block (28) corresponding to the top column (37). The inner layer (12) and the detection box (1) are provided with moving holes for the unidirectional electric push rod (36) to slide through.

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