A resonance angle detection type SPR microscopic imaging analyzer

Abstract

The application provides a resonance angle detection type SPR microscopic imaging analyzer and relates to the technical field of microscopic imaging analysis, and comprises a detection box, a control screen is fixedly installed on the side of the detection box, the front of the detection box is provided with an entrance, a box door is slidably installed on the outer surface of the entrance of the detection box, a conveying assembly is arranged outside the entrance of the detection box, a plurality of frame assemblies are arranged in the conveying assembly, a processed sample is placed into a corresponding specification collection tube, the collection tube is placed into a plug-in frame closest to the detection box, the frame assembly is sent into the detection box through the conveying assembly, the sample solution in the corresponding collection tube is sucked and sent into a detection cavity through a detection assembly, the sample solution is detected through a detection module, waste liquid is discharged after detection, and the pipeline and the detection cavity are automatically cleaned, the device can continuously detect and use, and the convenience and efficiency of detection are improved.

Description

Technical Field

[0001] This invention relates to the field of microscopic imaging analysis technology, specifically to a resonance angle detection type SPR microscopic imaging analyzer. Background Technology

[0002] The SPR microscopic imaging analyzer employs a Kretschmann prism coupling structure. P-polarized light emitted from a He / Ne laser is incident at a specific angle onto the gold-plated sensor chip interface at the bottom of the prism, generating an evanescent wave upon total internal reflection. When the wave vector of the evanescent wave perfectly matches the surface plasmonic wave vector of the free electrons oscillating on the gold film surface, energy coupling occurs, significantly attenuating the intensity of the reflected light and forming a resonance valley. The core structure of the SPR microscopic imaging analyzer can be divided into two main modules: the core optical hardware module and the hyperspectral microscopic imaging unit. The Kretschmann-type SPR sensing platform includes a high-brightness broadband fiber optic coupling lamp, a dispersion-free collimator, a Wollaston polarizer, and an isosceles right-angled glass prism, which, together with the SPR chip and refractive index matching liquid, generates p-polarized light and excites the SPR effect. The hyperspectral microscopic imaging unit consists of an achromatic imaging lens and a high-sensitivity CCD / near-infrared camera, capable of acquiring full-chip two-dimensional SPR images with a spatial resolution up to 14 μm, enabling visualization of surface signals.

[0003] Supporting functional systems: Microfluidic sample delivery system: Includes a peristaltic pump and multi-channel fluid tubing, with a flow rate continuously adjustable from 0-1000 μL / min, supporting stable sample delivery and dynamic reaction monitoring. Control and data processing unit: Composed of an industrial computer and supporting software, capable of real-time acquisition of image data, calculation of SPR angular displacement, and output of molecular interaction dynamics and affinity parameters.

[0004] In the existing technology, SPR microscopic imaging analyzers are divided into multi-chamber microfluidic chip designs, equipped with four or more independent and isolated detection chambers, each chamber corresponding to a dedicated SPR detection site, and single detection chamber sharing designs, in which all samples are sequentially introduced into the same detection chamber to complete the detection. However, whether it is a single chamber or a multi-chamber design, liquid needs to be introduced for detection and then cleaned, which is inefficient. In addition, the sample introduction methods are divided into manual and automatic, which cannot be well coordinated with the chamber cleaning time. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a resonance angle detection-based SPR microscopic imaging analyzer to solve the problems mentioned in the background section. To achieve the above objectives, the present invention provides the following technical solution: a resonance angle detection type SPR microscopic imaging analyzer, comprising: a detection box, a control panel fixedly mounted on the side of the detection box, an entrance provided on the front of the detection box, a door slidably mounted on the outer surface of the entrance, a conveying assembly provided on the outer side of the entrance, multiple sets of frame assemblies disposed inside the conveying assembly, and a detection assembly disposed inside the detection box; the conveying assembly includes a rotating frame, two sets of rotating frames are provided, the two sets of rotating frames are fixed on both sides of the outer end of the entrance of the detection box, a rotating plate is rotatably mounted on the inner surface of the top of the rotating frame, rotating blocks are fixed at both ends of the rotating plate, a slide rail is provided between the two rotating blocks, the slide rail is fixed on the rotating frame, and the slide rail is perpendicular to the entrance of the detection box; the frame assembly includes a plug-in frame. The plug-in frame has card frames fixed on both sides, which slide and engage with the rotating block and slide rail. A middle plate is fixed inside the middle of the card frame. Six insertion holes are equidistantly opened on the top of the card frame and the upper and lower surfaces of the middle plate. A data acquisition tube is inserted into each insertion hole. The detection component includes a control box, which is fixed at the top of the detection box. Six detection modules are fixed at the top of the detection box. A detection cavity is fixed at the upper end of each detection module. The detection modules and detection cavities correspond to the positions of the six data acquisition tubes inside the plug-in frame. An industrial vision recognition monitoring module is set inside the detection cavity. The detection module is an SPR sensor chip, and the sample is directly loaded onto the gold film functional modification area of ​​the gold-plated SPR sensor chip for detection.

[0006] Furthermore, the conveying assembly also includes: a vertical plate, a moving groove, a bidirectional screw, a screw block, a connecting rod, and a push plate. The vertical plate is fixed on the side of the rotating frame away from the detection box. A moving groove is formed on the surface of the vertical plate, and a bidirectional screw is rotatably installed inside the moving groove. Screw blocks are threaded onto both ends of the bidirectional screw. A connecting rod is rotatably installed on the outer surface of the screw block. The other ends of the two connecting rods are rotatably connected to a push plate. The push plate is on the same plane as the top of the insertion frame. A second motor is fixed on the outer side of the vertical plate corresponding to the position of the bidirectional screw, and the output end of the second motor is fixedly connected to the bidirectional screw.

[0007] Furthermore, connecting frames are fixed at both ends of the push plate, a limiting frame is fixed at the top of the rotating frame, a movable plate is slidably connected inside the limiting frame, one end of the movable plate is in contact with the connecting frame, and a rotating rod is rotatably connected to the other end of the movable plate, and the other end of the rotating rod is rotatably connected to the outer surface of the door; wherein, a first motor is fixed at the outer end of the rotating frame corresponding to the position of the rotating plate's rotation axis, and the output end of the first motor is fixedly connected to the rotating plate's rotation axis.

[0008] Furthermore, the testing box has sliding frames fixed on both sides of the door, and the door slides along the sliding frames. A second spring is fixed on the top of the sliding frame, and the bottom of the second spring is fixedly connected to the door. A baffle is slidably inserted into the bottom of the testing box at the entrance, and a first spring is fixed between the top of the baffle and the bottom of the testing box.

[0009] Furthermore, the frame assembly also includes: a middle plate, a circular plate, a movable notch, a protrusion, and a fourth spring. The middle plate has movable notches on both sides corresponding to each insertion hole. A circular plate is slidably connected inside the insertion hole of the middle plate. A protrusion is fixed to both sides of the circular plate through the movable notch. A fourth spring is fixed to both sides of the protrusion, and the other end of the fourth spring is fixedly connected to the movable notch. A first electric push rod is fixed at the top of the vertical plate corresponding to the position of the last insertion frame, and a push frame is fixed at the extended end of the first electric push rod. The push frame is in contact with the protrusions on both sides of the middle plate. A collection box is fixed at the bottom of the vertical plate corresponding to the position of the push frame.

[0010] Furthermore, the detection assembly also includes: a sleeve, an insertion tube, a fixing frame, and a suction pump. The fixing frame is provided on the side of the detection chamber facing the inlet of the detection box, and the fixing frame is fixed to the inner wall of the detection box. The fixing frame corresponds to the top of the insertion frame that enters the detection box. The fixing frame is fixedly connected to each detection chamber through a pipe. The bottom of the fixing frame is fixed with a sleeve corresponding to the position of each collection tube, and the bottom of the sleeve is slidably inserted with an insertion tube. The fixing frame has a chamber inside, and the pipe of the detection chamber and the sleeve are connected through the chamber. The fixing frame is fixed with a suction pump on the outside of each chamber, and the insertion tube is inserted from the top of the collection tube.

[0011] Furthermore, a connecting frame is slidably fitted to the bottom of the insertion tube, and the connecting frame is fixed to the bottom of the fixing frame. A stop block is slidably inserted inside the connecting frame, and a delivery tube is slidably inserted to one side of the stop block. A storage tank is fixed inside the detection box, and one end of the delivery tube is fixedly connected to the storage tank. A connecting channel is provided on the top of the stop block. When the stop block is located inside the connecting frame, the delivery tube is connected to the insertion tube through the connecting channel.

[0012] Furthermore, a stop plate is fixed to the bottom of the stop block, and the stop plate is in pressure contact with the top of the collection tube. A fifth spring is fixed to the side of the stop block facing the storage tank, and the other end of the fifth spring is fixedly connected to the storage tank. A slide rod is slidably inserted into the bottom of the storage tank. A roller is fixed to the end of the slide rod facing the inlet, and the roller is in pressure contact with the insertion frame. A third spring is sleeved on the other end of the slide rod. A contact switch is fixed inside the detection box at the position corresponding to the slide rod. After the insertion frame enters the detection box, the tail end of the slide rod contacts the contact switch.

[0013] Furthermore, the top of the fixing frame is slidably connected to the position of each insertion tube, the bottom of the insertion rod is fixedly connected to the top of the insertion tube, the top of the insertion rod is provided with a top plate, the bottom of the top plate is provided with a sliding groove, and a slider is slidably connected inside the sliding groove. The insertion rod is fixedly connected to the slider. The top of the top plate is provided with a pressure plate, and the pressure plate is provided with a slot corresponding to the position of each top plate. Telescopic plates are fixed on both sides of the top plate, and the bottom of the telescopic plates is fixedly connected to a stop block. The top of the control box is fixed with a second electric push rod, and the extended end of the second electric push rod is fixedly connected to the pressure plate. A sixth spring is sleeved on the outer surface of the insertion rod, and the two ends of the sixth spring are fixedly connected to the slider and the fixing frame.

[0014] Furthermore, an outlet pipe is fixed to the tail end of the detection chamber, a switching seat is fixed to the outlet end of the outlet pipe, a piston frame is slidably installed inside the switching seat, an mounting bracket is fixed to the outer end of the piston frame, and the other end of the mounting bracket is fixedly connected to the storage end of the telescopic plate; wherein, two return pipes are fixed to the bottom of the switching seat, a first recycling box is fixed to the bottom of one of the return pipes inside the detection box, and a second recycling box is fixed to the bottom of the other return pipe inside the detection box.

[0015] The beneficial effects of this invention are: 1. In this invention, when the frame assembly enters the detection station inside the detection box, it squeezes the roller, causing the slide rod to move backward until it contacts the contact switch. This triggers the second electric push rod, which drives the pressure plate downward. Here, the location inside the insertion frame where the data collection tube is inserted squeezes the abutment plate upon entering the detection box, causing the stop block to move out of the connecting frame. The telescopic plate drives the top plate to move and insert into the slot of the pressure plate. The slider slides along the groove to maintain connection with the insertion rod. Simultaneously, through the connection of the mounting bracket, the piston frame moves along the inside of the switching seat. The inside of the switching seat can be understood as a valve structure. The piston frame moving from one end to the other allows for the alternating opening of the two return pipes, thus enabling the detection of data collection tubes. In the first position, the return pipe connects to the first recovery tank and the detection chamber for liquid recovery after detection. In the position without the collection tube, the stop does not move and continues to block the insertion tube. The connection between the delivery pipe and the insertion tube is achieved through the connection channel of the stop. The top plate at the top of the insertion rod is not connected to the pressure plate, so the pressure plate will not cause the insertion rod at that position to descend or the insertion tube to extend. At this time, the cleaning liquid in the storage tank is sent into the insertion tube and the sleeve along the delivery pipe and the connection channel, and finally enters the detection chamber for rinsing. At this time, the switching seat connects the return pipe of the second recovery tank to the delivery pipe of the detection chamber, thereby recovering the cleaning liquid and preventing it from mixing with the sample liquid.

[0016] 2. This invention selects the corresponding detection chamber and connects it to the number of samples to be tested within the frame component, and draws the samples into the detection chamber for testing. Without the collection tube, the station maliciously maintains the sealing protection of the detection chamber or the flushing process of the detection chamber, thereby ensuring that the device can be used continuously. It also automatically adjusts according to the number of detection chambers required for each test, realizes the synchronous extraction of sample liquid from the collection tube, and cleans the pipeline and the inside of the detection chamber in a timely manner.

[0017] 3. This invention uses the circular plate of the intermediate plate for limiting the position, which facilitates the continuous use of the frame assembly when switching the rotating blocks and plug-in frames at both ends in coordination with the rotation of the rotating plate. It also eliminates the need to consider the problem of inversion after rotation, thus facilitating the continuous use of the device. When the plug-in frame rotates to the outermost end with the rotating plate and rotating blocks, the first electric push rod drives the plug frame to move, squeezing the protrusion on the intermediate plate. This causes the circular plate to move along the plug hole of the intermediate plate, pushing the collection tube out from the bottom and into the collection box, thereby freeing up the plug-in frame and collecting the collection tube.

[0018] 4. This invention uses a first motor to drive the rotation of the rotating plate and the rotating blocks at both ends, which can rotate the plug-in frame inside the detection box to the outermost end for continued use. Here, the rotating block can be aligned with the slide rail, which facilitates the frame assembly to enter the detection box along the slide rail onto the rotating block for sample detection. The second motor drives the bidirectional screw to rotate, and the screw block slides along the moving groove in threaded engagement with the bidirectional screw. The connecting rod pushes the push plate to move, and the push plate pushes the outermost plug-in frame towards the detection box, which facilitates the plug-in frame and the collection tube to be sent into the corresponding detection station inside the detection box.

[0019] 5. As the push plate moves, the connecting frame presses against the moving plate. The moving plate moves along the limiting frame and drives the door to slide upward along the sliding frame via the rotating rod, opening the entrance of the test box and facilitating the entry of the frame assembly. When the rotating plate drives the insertion frame to rotate, it presses against the baffle at the bottom of the test box, thus maintaining the seal of the entrance of the test box with the door without interfering with the rotation of the insertion frame. As a result, the frame assembly can be used alternately and continuously with the conveying assembly, avoiding the inconvenience of repeated manual adjustments.

[0020] 6. In this invention, the processed sample is placed inside a collection tube of the corresponding specification. The collection tube is placed inside the plug-in frame closest to the detection chamber. The frame assembly is sent into the detection chamber by the conveying component. The sample solution inside the corresponding collection tube is aspirated and sent into the detection chamber by the detection component. The sample solution is detected by the detection module. After the detection is completed, the waste liquid is discharged, and the pipeline and detection chamber are automatically cleaned. The device can be used continuously for detection, improving the convenience and efficiency of detection. Attached Figure Description

[0021] Figure 1This is a schematic diagram of the overall structure of a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 2 This is a schematic diagram of the internal structure of the detection chamber in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 3 This is a schematic diagram of the transport component structure in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 4 This is a schematic diagram of the connection between the frame assembly and the rotating frame in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 5 This is a schematic diagram of the connection between the frame assembly and the slide rail in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 6 This is a schematic diagram of the frame component structure in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 7 This is a schematic diagram of the connection between the slide bar and the contact switch in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 8 This is a schematic diagram of the separation of the connecting frame and the stop block in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 9 This is a schematic diagram of the internal structure of the connecting frame in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 10 This is a schematic diagram of the detection component structure in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; Figure 11 This is a schematic diagram showing the connection between the detection cavity and the first and second recovery boxes in a resonance angle detection type SPR microscopic imaging analyzer according to an embodiment of this disclosure; As shown in the figure: 1. Detection box; 11. Control panel; 12. Collection box; 13. Baffle; 14. First spring; 15. Sliding frame; 16. Second spring; 17. Box door; 18. Contact switch; 19. Sliding rod; 110. Roller; 111. Third spring; 2. Conveying assembly; 21. Rotating frame; 22. First motor; 23. Rotating plate; 24. Vertical plate; 25. Moving trough; 26. Second motor; 27. Bidirectional screw; 28. Screw block; 29. ​​Rotating rod; 210. Push plate; 211. Connecting frame; 212. Limiting frame; 213. Moving plate; 214. Connecting rod; 215. Rotating block; 216. First electric push rod; 217. Push frame; 3. Frame assembly; 31. Connector frame; 32. Socket; 33. Middle plate; 34. Slide rail; 35. Card frame; 36. Acquisition tube; 37. Circular plate; 38. Moving notch; 39. Protrusion; 310. Fourth spring; 4. Detection component; 41. Connecting frame; 42. Stop block; 43. Connecting channel; 44. Conveying pipe; 45. Storage tank; 46. Fifth spring; 47. Telescopic plate; 48. Sleeve; 49. Support plate; 410. Insertion tube; 411. Fixing frame; 412. Suction pump; 413. Second electric push rod; 414. Pressure plate; 415. Slot; 416. Top plate; 417. Slide groove; 418. Slider; 419. Insertion rod; 420. Sixth spring; 421. Control box; 422. Detection module; 423. Detection chamber; 424. Delivery pipe; 425. Switching seat; 426. Piston frame; 427. Mounting frame; 428. Return pipe; 429. First recycling bin; 430. Second recycling bin. Detailed Implementation

[0022] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0023] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 6 , Figure 7 and Figure 8As shown, this disclosure proposes a resonance angle detection type SPR microscopic imaging analyzer, comprising: a detection box 1, a control screen 11 fixedly mounted on the side of the detection box 1, an inlet on the front of the detection box 1, a door 17 slidably mounted on the outer surface of the inlet of the detection box 1, a conveying assembly 2 on the outer side of the inlet of the detection box 1, multiple sets of frame assemblies 3 arranged inside the conveying assembly 2, and a detection assembly 4 arranged inside the detection box 1; the conveying assembly 2 includes a rotating frame 21, two sets of the rotating frame 21 are provided, the two sets of the rotating frame 21 are fixed on both sides of the outer end of the inlet of the detection box 1, and the inner surface of the top of the rotating frame 21 is... A rotating plate 23 is rotatably mounted on the surface. Rotating blocks 215 are fixed to both ends of the rotating plate 23. A slide rail 34 is provided between the two rotating blocks 215. The slide rail 34 is fixed to the rotating frame 21 and is perpendicular to the inlet of the detection box 1. The frame assembly 3 includes a plug-in frame 31. Clip frames 35 are fixed to both sides of the plug-in frame 31. The clip frames 35 are slidably engaged with the rotating blocks 215 and the slide rail 34. A middle plate 33 is fixed inside the middle of the clip frame 35. Six insertion holes 32 are equidistantly opened on the top of the clip frame 35 and the upper and lower surfaces of the middle plate 33. A data acquisition tube 36 is inserted into the socket 32; the detection assembly 4 includes a control box 421, which is fixed to the top of the detection box 1. Six detection modules 422 are fixed to the top of the detection box 1, and a detection cavity 423 is fixed to the upper end of each detection module 422. The detection modules 422 and the detection cavity 423 correspond to the positions of the six data acquisition tubes 36 in the insertion frame 31. An industrial vision recognition monitoring module is installed inside the detection cavity 423; the detection module 422 is an SPR sensor chip, and the sample is directly loaded onto the gold-plated SPR sensor chip. For the detection of gold film functional modification areas, when using the device, the processed sample is placed inside the corresponding size collection tube 36, and the collection tube 36 is placed inside the plug frame 31 closest to the detection box 1. The frame assembly 3 is sent into the detection box 1 through the delivery assembly 2, and the sample solution inside the corresponding collection tube 36 is aspirated and sent into the detection chamber 423 through the detection assembly 4. The sample solution is detected by the detection module 422. After the detection is completed, the waste liquid is discharged, and the pipeline and detection chamber 423 are automatically cleaned. The device can be used continuously for detection, improving the convenience and efficiency of detection.

[0024] like Figure 2 , Figure 3 , Figure 4 and Figure 5As shown, in some embodiments, the conveying assembly 2 further includes: a vertical plate 24, a moving groove 25, a bidirectional screw 27, a screw block 28, a connecting rod 214, and a push plate 210. The vertical plate 24 is fixed on the side of the rotating frame 21 away from the detection box 1. A moving groove 25 is formed on the surface of the vertical plate 24, and a bidirectional screw 27 is rotatably installed inside the moving groove 25. Screw blocks 28 are threaded onto both ends of the bidirectional screw 27. A connecting rod 214 is rotatably installed on the outer surface of the screw block 28. The other ends of the two connecting rods 214 are rotatably connected to the push plate 210. The top of the push plate 210 is on the same plane as the top of the insertion frame 31. A second motor 26 is fixed on the outer side of the vertical plate 24 corresponding to the position of the bidirectional screw 27, and the output end of the second motor 26 is fixedly connected to the bidirectional screw 27. Connecting frames 211 are fixed at both ends of the push plate 210. The rotating frame 21... A limiting frame 212 is fixed at the top, and a movable plate 213 is slidably connected inside the limiting frame 212. One end of the movable plate 213 is in contact with the connecting frame 211, and the other end of the movable plate 213 is rotatably connected to a rotating rod 29, and the other end of the rotating rod 29 is rotatably connected to the outer surface of the door 17. A first motor 22 is fixed at the outer end of the rotating frame 21 corresponding to the position of the rotating shaft of the rotating plate 23, and the output end of the first motor 22 is fixedly connected to the rotating shaft of the rotating plate 23. Sliding frames 15 are fixed on both sides of the door 17 of the detection box 1, and the two sides of the door 17 slide along the sliding frames 15. A second spring 16 is fixed at the top of the sliding frame 15, and the bottom of the second spring 16 is fixedly connected to the door 17. A baffle 13 is slidably inserted at the bottom of the entrance of the detection box 1, and a first spring 14 is fixed between the top of the baffle 13 and the bottom of the detection box 1.

[0025] Understandably, by driving the rotating plate 23 and the rotating blocks 215 at both ends through the first motor 22, the insertion frame 31 inside the detection box 1 can be rotated to the outermost end for continued use. Here, the rotating block 215 can be aligned with the slide rail 34, thus facilitating the frame assembly 3 to enter the detection box 1 along the slide rail 34 onto the rotating block 215 for sample testing. The second motor 26 drives the bidirectional screw 27 to rotate, and the screw block 28 slides along the moving groove 25 in threaded engagement with the bidirectional screw 27. The connecting rod 214 pushes the push plate 210 to move, and the push plate 210 pushes the outermost insertion frame 31 towards the detection box 1, thus facilitating the insertion frame 31. Along with the collection tube 36, it is sent into the corresponding detection station inside the detection box 1. As the push plate 210 moves, the connecting frame 211 squeezes the moving plate 213. The moving plate 213 moves along the limiting frame 212 and drives the box door 17 to slide upward along the sliding frame 15 through the rotating rod 29, opening the entrance of the detection box 1 and facilitating the entry of the frame assembly 3. When the rotating plate 23 drives the plug frame 31 to rotate, it squeezes the baffle 13 at the bottom of the detection box 1. This can work with the box door 17 to keep the entrance of the detection box 1 sealed without interfering with the rotation of the plug frame 31. Thus, the frame assembly 3 can be used alternately and continuously with the conveying assembly 2, avoiding the inconvenience of repeated manual adjustments.

[0026] like Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 As shown, in some embodiments, the frame assembly 3 further includes: a middle plate 33, a circular plate 37, a movable notch 38, a protrusion 39, and a fourth spring 310. The middle plate 33 has movable notches 38 on both sides corresponding to each insertion hole 32. The circular plate 37 is slidably connected inside the insertion hole 32 of the middle plate 33. The protrusion 39 is fixed to both sides of the circular plate 37 through the movable notch 38. The fourth spring 310 is fixed to both sides of the protrusion 39, and the other end of the fourth spring 310 is fixedly connected to the movable notch 38. The top of the vertical plate 24 is fixed with a first electric push rod 216 at the position corresponding to the last insertion frame 31, and the extended end of the first electric push rod 216 is fixed with a push frame 217. The push frame 217 is in contact with the protrusion 39 on both sides of the middle plate 33. The bottom of the vertical plate 24 is fixed with a collection box 12 at the position corresponding to the push frame 217.

[0027] It is understandable that the two end holes 32 of the plug frame 31 can be used to insert the collection tube 36, and the circular plate 37 of the intermediate plate 33 limits the movement. This facilitates the continuous use of the frame assembly 3 when switching the two end rotating blocks 215 and the plug frame 31 with the rotation of the rotating plate 23, without having to consider the problem of inversion after rotation. This makes it convenient for the continuous use of the device. When the plug frame 31 rotates to the outermost end with the rotating plate 23 and the rotating block 215, the first electric push rod 216 drives the plug frame to move, which squeezes the protrusion 39 on the intermediate plate 33. This causes the circular plate 37 to move along the plug hole 32 of the intermediate plate 33, pushing the collection tube 36 out from the bottom and into the collection box 12, thereby freeing up the plug frame 31 and collecting the collection tube 36.

[0028] like Figure 7 , Figure 8 , Figure 9 , Figure 10 and Figure 11As shown, in some embodiments, the detection assembly 4 further includes: a sleeve 48, an insertion tube 410, a fixing frame 411, and a suction pump 412. The fixing frame 411 is provided on the side of the detection chamber 423 facing the inlet of the detection box 1, and the fixing frame 411 is fixed to the inner wall of the detection box 1. The fixing frame 411 corresponds to the top of the insertion frame 31 entering the detection box 1. The fixing frame 411 is fixedly connected to each detection chamber 423 through a pipe. A sleeve 48 is fixed at the bottom of the fixing frame 411 corresponding to the position of each collection tube 36, and the insertion tube 410 is slidably inserted into the bottom of the sleeve 48. A chamber is provided inside the fixing frame 411, and the pipe of the detection chamber 423 and the sleeve 48 communicate through the chamber. A suction pump 412 is fixed to the outside of each chamber of the fixing frame 411. The insertion tube 410 is inserted from the top of the collection tube 36. A connecting frame 41 is slidably sleeved at the bottom of the insertion tube 410, and the connecting frame 41 is fixed to the bottom of the fixing frame 411. A stop block 42 is slidably inserted inside the connecting frame 41. A delivery tube 44 is slidably inserted to one side of the stop block 42. A storage tank 45 is fixed inside the detection box 1, and one end of the delivery tube 44 is fixedly connected to the storage tank 45. A connecting channel 43 is opened at the top of the stop block 42. When the stop block 42 is inside the connecting frame 41, the delivery tube 44 is connected to the insertion tube 410 through the connecting channel 43. A backing plate is fixed at the bottom of the stop block 42. 49. The abutment 49 is in contact with the top of the collection tube 36 by pressing. A fifth spring 46 is fixed to the side of the stop block 42 facing the storage tank 45, and the other end of the fifth spring 46 is fixedly connected to the storage tank 45. A slide rod 19 is slidably inserted into the bottom of the storage tank 45. A roller 110 is fixed to one end of the slide rod 19 facing the inlet. The roller 110 is in contact with the insertion frame 31 by pressing. A third spring 111 is sleeved on the other end of the slide rod 19. A contact switch 18 is fixed inside the detection box 1 at the position corresponding to the slide rod 19. After the insertion frame 31 enters the detection box 1, the tail end of the slide rod 19 contacts the contact switch 18. The top of the fixing frame 411 corresponds to the position of each insertion tube 410. A slidable insertion rod 419 is provided, the bottom of which is fixedly connected to the top of the insertion tube 410. A top plate 416 is provided on the top of the insertion rod 419. A sliding groove 417 is provided at the bottom of the top plate 416, and a slider 418 is slidably connected inside the sliding groove 417. The insertion rod 419 is fixedly connected to the slider 418. A pressure plate 414 is provided on the top of the top plate 416. A slot 415 is provided on the pressure plate 414 corresponding to the position of each top plate 416. Telescopic plates 47 are fixed on both sides of the top plate 416, and the bottom of the telescopic plates 47 is fixedly connected to the stop block 42. A second electric push rod 413 is fixed on the top of the control box 421, and the extended end of the second electric push rod 413 is fixedly connected to the pressure plate 414.The insertion rod 419 has a sixth spring 420 sleeved on its outer surface. Both ends of the sixth spring 420 are fixedly connected to the slider 418 and the fixing frame 411. The tail end of the detection chamber 423 is fixed with an outlet pipe 424. The outlet end of the outlet pipe 424 is fixed with a switching seat 425. A piston frame 426 is slidably mounted inside the switching seat 425. An mounting frame 427 is fixed to the outer end of the piston frame 426. The other end of the mounting frame 427 is fixedly connected to the receiving end of the telescopic plate 47. Two return pipes 428 are fixed to the bottom of the switching seat 425. Inside the detection box 1, corresponding to the bottom of one of the return pipes 428, a first recycling box 429 is fixed to the bottom, and inside the detection box 1, corresponding to the bottom of the other return pipe 428, a second recycling box 430 is fixed to the bottom.

[0029] It should be noted that when the frame assembly 3 enters the detection station inside the detection box 1, it will squeeze the roller 110, causing the slide bar 19 to move backward until it contacts the contact switch 18, thereby triggering the second electric push rod 413 to drive the pressure plate 414 to move downward. Here, the position where the acquisition tube 36 is inserted inside the insertion frame 31 will squeeze the abutment plate 49 when it enters the detection box 1, causing the stop block 42 to move out of the connecting frame 41. The telescopic plate 47 drives the top plate 416 to move and insert into the slot 415 of the pressure plate 414. The slider 418 slides along the slide groove 417 to maintain the connection with the insertion rod 419. Simultaneously, through the connection of the mounting bracket 427, the piston bracket 426 moves along the inside of the switching seat 425. The inside of the switching seat 425 can be understood as a valve structure. The piston bracket 426 moving from one end to the other can realize the alternating opening of the two return pipes 428. Thus, at the position with the collection pipe 36, the return pipe 428 is connected to the first recovery box 429 and the detection chamber 423 for the recovery and treatment of the detected liquid. At the position without the collection pipe 36, the stop block 42 does not move and continues to block the insertion tube 410. And through the connection channel 43 of the stop block 42, The delivery pipe 44 is connected to the insertion tube 410. The top plate 416 at the top of the insertion rod 419 is not connected to the pressure plate 414. Therefore, the descent of the pressure plate 414 will not cause the insertion rod 419 at that position to descend or the insertion tube 410 to extend. At this time, the cleaning fluid in the storage tank 45 is sent into the insertion tube 410 and the sleeve 48 along the delivery pipe 44 and the connecting channel 43, and finally enters the detection chamber 423 to rinse it. At this time, the switching seat 425 connects the return pipe 428 of the second recovery box 430 with the delivery pipe 424 of the detection chamber 423, thereby recovering the cleaning fluid. To avoid mixing with the sample liquid, the corresponding detection chamber 423 is selected and connected to the frame component 3 according to the number of samples to be tested. The sample is then drawn into the detection chamber 423 for testing. There is no station without the collection tube 36 to maliciously maintain the sealing protection of the detection chamber 423 or to flush the detection chamber 423. This ensures that the device can be used continuously and automatically. The number of detection chambers 423 required for each test is automatically adjusted to achieve synchronous extraction of sample liquid from the collection tube 36 and timely cleaning of the pipeline and the inside of the detection chamber 423. In this solution, a monitoring module is also installed inside the detection chamber 423. When liquid enters the detection chamber 423, an electrical signal is emitted, causing the detection module 422 to start detection. The monitoring module adopts existing technologies such as industrial vision, visual intelligence, AI vision, visual inspection, and visual measurement, and uses sensors or miniature lenses for monitoring. The detection method of the detection module 422 is that the sample liquid comes into contact with the chip of the detection module 422, and the resonance angle image RA image is obtained by calculating pixel by pixel. The imaging is completed based on the SPR angle information. Compared with the traditional fixed angle monitoring of reflectivity, it can maintain a linear response to the change of surface mass density within a wide dynamic angle range of 8°, and can also significantly reduce stray light interference, improve imaging contrast and edge clarity. The detection technology meets the technical requirements of new material testing, metrology, related standardization, certification and accreditation services.

[0030] Working principle: When using the device, the processed sample is placed into the corresponding size collection tube 36. The collection tube 36 is placed into the insertion frame 31 closest to the detection box 1. The first motor 22 drives the rotating plate 23 and the rotating blocks 215 at both ends to rotate, which can rotate the insertion frame 31 inside the detection box 1 to the outermost end for continued use. Here, the rotating blocks 215 can be aligned with the slide rail 34, which facilitates the frame assembly 3 to enter the detection box 1 along the slide rail 34 onto the rotating blocks 215 for sample detection. The second motor 26 drives the bidirectional screw 27 to rotate. The screw block 28 and the bidirectional screw 27 are threaded together and slide along the moving groove 25. The connecting rod 214 pushes the push plate 210 to move, and the push plate 210 pushes the outermost insertion frame 31 towards the detection box 1. The insertion frame 31, along with the collection tube 36, is conveniently fed into the corresponding testing station inside the testing box 1. As the push plate 210 moves, the connecting frame 211 presses against the moving plate 213. The moving plate 213 moves along the limiting frame 212, which in turn drives the box door 17 to slide upward along the sliding frame 15 via the rotating rod 29, opening the entrance of the testing box 1 and facilitating the entry of the frame assembly 3. When the rotating plate 23 drives the insertion frame 31 to rotate, it presses against the baffle 13 at the bottom of the testing box 1. This, in conjunction with the box door 17, keeps the entrance of the testing box 1 sealed without interfering with the rotation of the insertion frame 31. Thus, the frame assembly 3 can be used alternately and continuously with the conveying assembly 2, avoiding the inconvenience of repeated manual adjustments. The insertion holes 32 at both ends of the insertion frame 31 can be used to insert the collection tube 36, and through the middle... The circular plate 37 of the intermediate plate 33 is positioned to facilitate continuous use of the frame assembly 3 when switching between the rotating blocks 215 and the insertion frame 31 at both ends of the rotating plate 23, without needing to consider the problem of inversion after rotation. This facilitates continuous use of the device. When the insertion frame 31 rotates to its outermost end with the rotating plate 23 and the rotating block 215, the first electric push rod 216 drives the insertion frame to move, pressing the protrusion 39 on the intermediate plate 33. This causes the circular plate 37 to move along the insertion hole 32 of the intermediate plate 33, pushing the collection tube 36 out from the bottom and into the collection box 12, thereby freeing up the insertion frame 31 and collecting the collection tube 36. The detection assembly 4 then aspirates the sample solution inside the corresponding collection tube 36 and sends it into the detection chamber 423. The testing module 422 tests the sample solution. When the frame assembly 3 enters the testing station inside the testing chamber 1, it squeezes the roller 110, causing the slide rod 19 to move backward until it contacts the contact switch 18. This triggers the second electric push rod 413, which drives the pressure plate 414 to move downward. Here, the position inside the insertion frame 31 where the collection tube 36 is inserted will squeeze the abutment plate 49 when it enters the testing chamber 1, causing the stop block 42 to move out of the connecting frame 41. The telescopic plate 47 drives the top plate 416 to move and insert into the slot 415 of the pressure plate 414. The slider 418 slides along the slide groove 417 to maintain the connection with the insertion rod 419. At the same time, through the connection of the mounting bracket 427, the piston bracket 426 moves along the inside of the switching seat 425. The inside of the switching seat 425 can be understood as a valve structure.The piston holder 426 can move from one end to the other to alternately open the two return pipes 428. At locations with the collection pipe 36, the return pipe 428 is connected to the first recovery box 429 and the detection chamber 423 for liquid recovery after detection. At locations without the collection pipe 36, the stop block 42 does not move, continuing to block the insertion tube 410. The connection channel 43 of the stop block 42 connects the delivery pipe 44 to the insertion tube 410. The top plate 416 of the insertion rod 419 is not connected to the pressure plate 414, so the descent of the pressure plate 414 will not cause the insertion rod 419 to descend or the insertion tube 410 to extend at that location. At this time, the cleaning fluid in the storage tank 45 is sent along the delivery pipe 44 and the connection channel 43 into the insertion tube 410 and the sleeve 48, finally entering the detection chamber 423 for rinsing. At this time, the switching seat 425 connects the return pipe 428 of the second recovery box 430 with the delivery pipe 424 of the detection chamber 423, thereby recovering the cleaning fluid and preventing it from mixing with the sample liquid. For the number of samples to be tested within the frame assembly 3, the corresponding detection chamber 423 is selected and connected to it, and the sample is drawn into the detection chamber 423 for testing. Without the collection tube 36, the station maintains the sealing protection of the detection chamber 423 or performs flushing, thus ensuring continuous use of the device. The system automatically adjusts according to the number of detection chambers 423 required for each test, synchronously extracting sample liquid from the collection tube 36 and promptly cleaning the pipeline and the inside of the detection chamber 423. The device can be used continuously, improving the convenience and efficiency of testing.

[0031] The foregoing has shown and described the basic principles and main features of the present invention and its advantages. It will be apparent to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention.

[0032] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A resonance angle detection type SPR microscopic imaging analyzer, characterized in that, include: The test box (1) has a control panel (11) fixedly installed on its side. The test box (1) has an entrance on its front. The test box (1) has a door (17) slidably installed on the outer surface of the entrance. The test box (1) has a conveying assembly (2) on its outer side. The conveying assembly (2) has multiple sets of frame assemblies (3) inside. The test box (1) has a test assembly (4) inside. The conveying assembly (2) includes a rotating frame (21), which has two sets. The two sets of rotating frames (21) are fixed on both sides of the outer end of the inlet of the detection box (1). A rotating plate (23) is rotatably installed on the inner surface of the top of the rotating frame (21). A rotating block (215) is fixed at both ends of the rotating plate (23). A slide rail (34) is provided between the two rotating blocks (215). The slide rail (34) is fixed on the rotating frame (21) and is perpendicular to the inlet of the detection box (1). The frame assembly (3) includes a plug-in frame (31), with card frames (35) fixed on both sides of the plug-in frame (31). The card frames (35) are slidably engaged with the rotating block (215) and the slide rail (34). A middle plate (33) is fixed inside the middle of the card frame (35). Six insertion holes (32) are equidistantly opened on the top of the card frame (35) and the upper and lower end surfaces of the middle plate (33). A collection tube (36) is inserted into the insertion hole (32). The detection component (4) includes a control box (421), which is fixed at the top inside the detection box (1). Six detection modules (422) are fixed at the top of the detection box (1). A detection cavity (423) is fixed at the upper end of each detection module (422). The detection modules (422) and the detection cavity (423) correspond to the positions of the six acquisition tubes (36) in the plug-in frame (31). An industrial vision recognition monitoring module is installed inside the detection cavity (423). The detection module (422) is an SPR sensor chip, and the sample is directly loaded onto the gold film functional modification area of ​​the gold-plated SPR sensor chip for detection.

2. The SPR microscopic imaging analyzer with resonance angle detection according to claim 1, characterized in that, The conveying assembly (2) further includes: The vertical plate (24), the moving groove (25), the bidirectional screw (27), the screw block (28), the connecting rod (214), and the push plate (210) are fixed on the side of the rotating frame (21) away from the detection box (1). The moving groove (25) is opened on the surface of the vertical plate (24), and the bidirectional screw (27) is rotatably installed inside the moving groove (25). The two ends of the bidirectional screw (27) are threaded with the screw block (28). The outer surface of the screw block (28) is rotatably installed with the connecting rod (214). The other ends of the two connecting rods (214) are rotatably connected with the push plate (210). The push plate (210) and the top of the plug frame (31) are on the same plane. The second motor (26) is fixed on the outer side of the vertical plate (24) at the position corresponding to the bidirectional screw (27), and the output end of the second motor (26) is fixedly connected to the bidirectional screw (27).

3. The SPR microscopic imaging analyzer with resonance angle detection according to claim 2, characterized in that, The push plate (210) is fixed with connecting frames (211) at both ends, and the top of the rotating frame (21) is fixed with a limiting frame (212). A moving plate (213) is slidably connected inside the limiting frame (212). One end of the moving plate (213) is pressed against the connecting frame (211), and the other end of the moving plate (213) is rotatably connected with a rotating rod (29). The other end of the rotating rod (29) is rotatably connected to the outer surface of the box door (17). The outer end of the rotating frame (21) is fixed with a first motor (22) at the position corresponding to the rotation shaft of the rotating plate (23), and the output end of the first motor (22) is fixedly connected to the rotation shaft of the rotating plate (23).

4. The SPR microscopic imaging analyzer with resonance angle detection according to claim 3, characterized in that, The detection box (1) has sliding frames (15) fixed on both sides of the box door (17). The two sides of the box door (17) slide along the sliding frames (15). A second spring (16) is fixed on the top of the sliding frame (15). The bottom of the second spring (16) is fixedly connected to the box door (17). The detection box (1) is slidably connected to a baffle (13) at the bottom of the inlet, and a first spring (14) is fixed between the top of the baffle (13) and the bottom of the detection box (1).

5. The SPR microscopic imaging analyzer with resonance angle detection according to claim 4, characterized in that, The framework component (3) also includes: The middle plate (33), the circular plate (37), the movable notch (38), the protrusion (39), and the fourth spring (310) are provided. The middle plate (33) has movable notches (38) on both sides corresponding to each insertion hole (32). The circular plate (37) is slidably connected inside the insertion hole (32) of the middle plate (33). The protrusion (39) is fixed through the movable notch (38) on both sides of the circular plate (37). The fourth spring (310) is fixed on both sides of the protrusion (39), and the other end of the fourth spring (310) is fixedly connected to the movable notch (38). The vertical plate (24) is fixed with a first electric push rod (216) at the position corresponding to the last end insertion frame (31) at the top, and a push frame (217) is fixed at the extended end of the first electric push rod (216). The push frame (217) is pressed against the protrusions (39) on both sides of the middle plate (33). A collection box (12) is fixed at the bottom of the vertical plate (24) at the position corresponding to the push frame (217).

6. The SPR microscopic imaging analyzer with resonance angle detection according to claim 5, characterized in that, The detection component (4) also includes: The device includes a sleeve (48), an insertion tube (410), a fixing frame (411), and a suction pump (412). The fixing frame (411) is provided on the side of the detection chamber (423) facing the inlet of the detection box (1), and the fixing frame (411) is fixed on the inner wall of the detection box (1). The fixing frame (411) corresponds to the top of the insertion frame (31) that enters the detection box (1). The fixing frame (411) is fixedly connected to each detection chamber (423) through a pipe. The bottom of the fixing frame (411) is fixed with a sleeve (48) corresponding to the position of each collection tube (36), and the insertion tube (410) is slidably inserted into the bottom of the sleeve (48). The fixing frame (411) has a chamber inside, and the pipe of the detection chamber (423) and the sleeve (48) are connected through the chamber. The fixture (411) is fixed with a suction pump (412) on the outside of each chamber, and the cannula (410) is inserted from the top of the collection tube (36).

7. The SPR microscopic imaging analyzer with resonance angle detection according to claim 6, characterized in that, The bottom of the insertion tube (410) is slidably fitted with a connecting frame (41), and the connecting frame (41) is fixed to the bottom of the fixing frame (411). A stop block (42) is slidably inserted inside the connecting frame (41), and a delivery pipe (44) is slidably inserted on one side of the stop block (42). A storage tank (45) is fixed inside the detection box (1), and one end of the delivery pipe (44) is fixedly connected to the storage tank (45). The top of the stop (42) is provided with a connecting channel (43). When the stop (42) is located inside the connecting frame (41), the delivery pipe (44) is connected to the insertion pipe (410) through the connecting channel (43).

8. The SPR microscopic imaging analyzer with resonance angle detection according to claim 7, characterized in that, The bottom of the stop (42) is fixed with a stop plate (49), the stop plate (49) is pressed against the top of the collection tube (36), and a fifth spring (46) is fixed on the side of the stop (42) facing the storage tank (45), and the other end of the fifth spring (46) is fixedly connected to the storage tank (45). The storage tank (45) has a sliding rod (19) slidably inserted at the bottom. A roller (110) is fixed at one end of the sliding rod (19) facing the inlet. The roller (110) is pressed against the insertion frame (31). A third spring (111) is sleeved at the other end of the sliding rod (19). A contact switch (18) is fixed inside the detection box (1) at the position corresponding to the sliding rod (19). After the insertion frame (31) enters the detection box (1), the tail end of the sliding rod (19) contacts the contact switch (18).

9. The resonance angle detection type SPR microscopic imaging analyzer according to claim 8, characterized in that, The top of the fixing frame (411) is slidably connected to the position of each insertion tube (410), and the bottom of the insertion rod (419) is fixedly connected to the top of the insertion tube (410). The top of the insertion rod (419) is provided with a top plate (416), and the bottom of the top plate (416) is provided with a sliding groove (417), and a slider (418) is slidably connected inside the sliding groove (417). The insertion rod (419) and the slider (418) are fixedly connected. The top plate (416) is provided with a pressure plate (414), and the pressure plate (414) is provided with a slot (415) corresponding to the position of each top plate (416). The top plate (416) is fixed with telescopic plates (47) on both sides, and the bottom of the telescopic plates (47) is fixedly connected to the stop block (42). The top of the control box (421) is fixed with a second electric push rod (413), and the extended end of the second electric push rod (413) is fixedly connected to the pressure plate (414). The outer surface of the insertion rod (419) is fitted with a sixth spring (420), and the two ends of the sixth spring (420) are fixedly connected to the slider (418) and the fixing frame (411).

10. The SPR microscopic imaging analyzer with resonance angle detection according to claim 9, characterized in that: The detection chamber (423) is fixed with an outlet tube (424), and the outlet end of the outlet tube (424) is fixed with a switching seat (425). A piston frame (426) is slidably installed inside the switching seat (425), and an installation frame (427) is fixed to the outer end of the piston frame (426). The other end of the installation frame (427) is fixedly connected to the storage end of the telescopic plate (47). The bottom of the switching seat (425) is fixed with two return pipes (428), the bottom of the detection box (1) is fixed with a first recycling box (429) corresponding to the bottom of one of the return pipes (428), and the bottom of the detection box (1) is fixed with a second recycling box (430) corresponding to the bottom of the other return pipe (428).

Images