A disposable sampling head back-insertion triggered, quantitative transfer, backflow prevention isolation and access interlocking detection structure and method for non-invasive electrochemical detection of liquid samples

Abstract

The application discloses a one-time sampling head back insertion triggering, quantitative transfer, backflow prevention isolation and access interlocking detection structure and method for non-invasive liquid sample electrochemical detection, and belongs to the technical field of liquid sample electrochemical detection. The structure comprises a detection host and a one-time sampling head. The detection host comprises a main control module, an access determination module, a driving execution module, a sampling head interface seat, a quantitative transfer channel, a backflow prevention isolation unit, an electrochemical detection bin, a result output module, a position detection piece, a state detection module and a waste liquid receiving area. After the sampling head is back inserted, the sample completes quantitative transfer through back insertion triggering, realizes backflow prevention isolation through a one-way isolation valve and a backflow prevention buffer cavity, is introduced into the electrochemical detection bin when the state is satisfied, and outputs a detection result. When the state is abnormal, the sampling head is locked and retry is prompted, so that sample introduction consistency, isolation stability and detection reliability are improved.

Description

Technical Field

[0001] This invention relates to the fields of non-invasive liquid sample electrochemical detection equipment, disposable sampling heads, quantitative sample transfer, backflow prevention isolation, and detection access interlock technology. In particular, it relates to a disposable sampling head re-insertion triggering, quantitative transfer, backflow prevention isolation, and access interlock detection structure and method for non-invasive liquid sample detection such as saliva, oral fluid, tears, sweat, or exhaled breath condensate. Background Technology

[0002] Non-invasive electrochemical detection of liquid samples typically requires sample collection before importing the sample into the electrochemical detection chamber. In home or community settings, issues such as inconsistent sampling head volume, improper insertion of the sampling head, incorrect insertion posture, insufficient sample transfer, sample backflow, and reuse of already used sampling heads are common, easily leading to unstable sample transfer volume, host interface contamination, or invalid result output.

[0003] In existing solutions, sample collection, sample transfer, and detection initiation are typically performed separately by users. The detection host cannot promptly confirm whether the sampling head has been correctly inserted, whether it has a legitimate identity, whether quantitative transfer has been completed, and whether the sample has been reliably introduced into the detection chamber. Even if the detection chamber itself has electrochemical detection capabilities, abnormalities in the front-end sampling and transfer status may still affect the reliability of the detection.

[0004] Therefore, it is necessary to provide a detection structure and method based on the re-insertion action of a disposable sampling head, which forms a closed loop of sampling, re-insertion, in-place detection, identity verification, interlock permission, quantitative transfer, anti-backflow isolation, and detection chamber introduction, so as to reduce user misoperation, sample backflow contamination, and invalid detection output. Summary of the Invention

[0005] The technical problem this invention aims to solve is: in non-invasive electrochemical detection of liquid samples, how to combine the sampling and re-insertion of the disposable sampling head, quantitative sample transfer, backflow prevention isolation, and detection access interlock, so that the detection host only allows detection after the sampling head is in place, identity verification is passed, the sample transfer status meets the requirements, and the detection chamber is fully imported.

[0006] To address the aforementioned technical problems, this invention provides a detection structure for non-invasive electrochemical detection of liquid samples, featuring a disposable sampling head insertion trigger, quantitative transfer, backflow prevention isolation, and access interlock. This structure includes a detection host and a disposable sampling head. The detection host includes a main control module, an access determination module, a drive execution module, a sampling head interface, a quantitative transfer channel, a backflow prevention isolation unit, an electrochemical detection chamber, a result output module, a positioning detection element, a status detection module, and a waste liquid receiving area. The disposable sampling head includes a sampling liquid storage area and an insertion trigger.

[0007] In one embodiment, after the disposable sampling head is re-inserted into the sampling head interface seat, the arrival detection component and the status detection module acquire the sampling head arrival status and sampling head status; when the insertion status, identity verification status and interlocking conditions are met, the access determination module controls the drive execution module to trigger quantitative transfer, so that the non-invasive liquid sample in the sampling liquid storage area enters the electrochemical detection chamber through the quantitative transfer channel and the anti-backflow isolation unit, and the excess liquid enters the waste liquid receiving area.

[0008] In one embodiment, the quantitative transfer and backflow prevention isolation structure of the disposable sampling head includes a sampling port, a sampling aspiration chamber, a quantitative metering chamber, a back-insertion trigger post, a push-transfer element, a one-way isolation valve, a backflow prevention buffer chamber, an electrochemical detection chamber inlet, a waste liquid bypass, a vent, and a liquefying membrane. The sample is transferred downstream from the quantitative metering chamber by the back-insertion trigger post and the push-transfer element, while the one-way isolation valve and the backflow prevention buffer chamber prevent the sample from flowing back towards the sampling head.

[0009] In one embodiment, a detection host interface cavity, guide slope, limit step, insertion detection switch, identification contact, access interlock, crimping actuator, sealing ring, locking reset spring, anti-misinsertion block and positioning reference surface are provided between the disposable sampling head and the detection host to complete anti-misinsertion positioning, identification, positioning confirmation, sealing docking and detection access control.

[0010] Compared with existing technologies, the beneficial effects of this invention are as follows: First, it binds the disposable sampling head re-insertion action with the quantitative sample transfer action, reducing sample volume fluctuations caused by manual dripping; Second, it reduces the risk of sample backflow and host contamination through a one-way isolation valve, anti-backflow buffer chamber, hydrophobic membrane, and waste liquid bypass; Third, it reduces the risks of reverse insertion, misalignment, incomplete insertion, and reuse through an insertion detection switch, identification contact, anti-misinsertion block, and positioning reference surface; Fourth, it improves the detection reliability in home or grassroots scenarios by using status determination as a detection access condition. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the system structure for detecting the structure of the present invention; Figure 2 This is a schematic diagram of the quantitative transfer, backflow prevention isolation, and flow guiding structure within the disposable sampling head of the present invention; Figure 3 This is a schematic diagram of the interaction and access interlocking structure between the disposable sampling head and the interface cavity of the detection host of the present invention; Figure 4 This is a flowchart of the detection method of the present invention. Detailed Implementation

[0012] The specific embodiments of the present invention will now be described with reference to the accompanying drawings. These embodiments are used to explain the present invention and are not intended to limit the scope of protection of the present invention. Where there is no conflict, the technical features in the following embodiments can be combined with each other.

[0013] See Figure 1 The detection system of the present invention includes a detection host 100 and a disposable sampling head 111. The detection host 100 includes a main control module 101, an access determination module 102, a drive execution module 103, a sampling head interface 104, a quantitative transfer channel 105, an anti-backflow isolation unit 106, an electrochemical detection chamber 107, a result output module 108, a positioning detection element 109, a status detection module 110, and a waste liquid receiving area 114. The disposable sampling head 111 includes a sampling liquid storage area 112 and a re-insertion trigger element 113. After the liquid sample is sampled by the disposable sampling head 111, it is re-inserted into the sampling head interface 104 along with the disposable sampling head 111, and, with the cooperation of the main control module 101, the access determination module 102, and the drive execution module 103, is introduced into the electrochemical detection chamber 107 through the quantitative transfer channel 105 and the anti-backflow isolation unit 106.

[0014] Figure 1 The disposable sampling head 111 in the figure is represented by the system layer. Figure 3 The disposable sampling head 302 in the figure represents its local structure when it is engaged with the interface cavity of the detection host.

[0015] The electrochemical detection chamber 107 is equipped with a working electrode 115, a reference electrode 116, and an auxiliary electrode 117, which together constitute the electrochemical detection electrode system for liquid samples. Residual or excess liquid after detection enters the waste liquid receiving area 114. The positioning detection element 109 detects whether the disposable sampling head 111 has reached the predetermined position. The status detection module 110 detects sample transfer, backflow prevention isolation, and the status of the detection chamber introduction. The result output module 108 outputs the detection results, anomaly lockout prompts, or retry prompts.

[0016] See Figure 2 The quantitative transfer and backflow prevention isolation structure inside the disposable sampling head includes a sampling port 201, a sampling aspiration chamber 202, a quantitative metering chamber 203, a back insertion trigger post 204, a push-transfer component 205, a one-way isolation valve 206, a backflow prevention buffer chamber 207, an electrochemical detection chamber inlet 209, a waste liquid bypass 210, an exhaust port 211, and a liquefying membrane 212, and is provided with a flow guiding direction 213. Figure 2 Two hydrophore membranes 212 are installed in the exhaust path to reduce the risk of liquid spillage or backflow while allowing gas to be discharged.

[0017] The sample enters the sampling aspiration chamber 202 through the sampling port 201 and then enters the quantitative measurement chamber 203. When the disposable sampling head performs a re-insertion action, the re-insertion trigger post 204 triggers the push-transfer element 205 to actuate, and the sample in the quantitative measurement chamber 203 is pushed downstream of the one-way isolation valve 206 along the flow direction 213. The one-way isolation valve 206 is used to allow the sample to flow towards the anti-backflow buffer chamber 207 and to prevent the sample from flowing back towards the sampling port 201 when the compression is released, the sample is back-pressurized, or the sampling head is removed.

[0018] The anti-backflow buffer chamber 207 is used to buffer pressure fluctuations caused by quantitative transfer and to receive trace amounts of backflow liquid that may pass through the one-way isolation valve 206. After passing through the anti-backflow buffer chamber 207, the sample enters the electrochemical detection chamber inlet 209; excess liquid can be discharged through the waste liquid bypass 210, and gas can be discharged through the vent 211, thereby improving the consistency of sample introduction and the stability at the detection chamber inlet.

[0019] See Figure 3 The disposable sampling head 302 and the interface cavity 301 of the detection host are provided with a guide slope 303, a limiting step 304, an insertion detection switch 305, an identification contact 306, an access interlock 307, a crimping actuator 308, a sealing ring 309, a sampling head outlet 310, a detection chamber inlet 311, a locking reset spring 312, an anti-misinsertion stop 313, and a positioning reference surface 314. After the disposable sampling head 302 is inserted, it is guided by the guide slope 303 and positioned by the limiting step 304 and the positioning reference surface 314.

[0020] Insertion detection switch 305 is used to confirm whether the disposable sampling head 302 is inserted correctly, and identification contact 306 is used to identify the identity or usage status of the disposable sampling head 302. Anti-misinsertion stop 313 is used to limit incorrect insertion posture. After verification, access interlock 307 is activated by crimping actuator 308, causing sampling head outlet 310 to align with detection chamber inlet 311, and achieving a sealed connection through sealing ring 309; locking reset spring 312 is used to reset access interlock 307 after crimping is released.

[0021] See Figure 4 The detection method of this invention includes: 401 sampling head sampling, 402 sampling head re-insertion, 403 insertion in place detection, 404 access identity verification, 405 interlocking permission triggering, 406 quantitative sample transfer, 407 anti-backflow isolation, 408 importing into the detection chamber, and 409 status determination. When the status is normal, 410 is executed to allow detection, and 411 is executed to output the detection result after the detection is completed; when the status is abnormal, 412 is executed to lock the abnormality and prompt for retry.

[0022] The status determination 409 can include a comprehensive determination of the sampling head insertion status, identity verification status, quantitative sample transfer status, backflow prevention isolation status, and electrochemical detection chamber introduction status. If any status does not meet the preset conditions, the main control module 101 controls the detection process to lock through the access determination module 102 and outputs a retry prompt through the result output module 108.

[0023] This invention can be used for the electrochemical detection of non-invasive fluid samples such as saliva, oral fluid, tears, sweat, or exhaled breath condensate. The device output can provide sample status indications, test result indications, or retest assessments; for health monitoring or risk warning applications, the output results are not considered as disease diagnosis conclusions.

Claims

1. A disposable sampling head re-insertion triggering, quantitative transfer, backflow prevention isolation, and access interlocking detection structure for non-invasive electrochemical detection of liquid samples, characterized in that, The system includes a detection host and a disposable sampling head. The detection host includes a main control module, an access determination module, a drive execution module, a sampling head interface, a quantitative transfer channel, an anti-backflow isolation unit, an electrochemical detection chamber, a result output module, a positioning detection element, a status detection module, and a waste liquid receiving area. The disposable sampling head includes a sampling liquid storage area and a re-insertion trigger element. The sampling head interface is connected to the quantitative transfer channel, the quantitative transfer channel is connected to the anti-backflow isolation unit, the anti-backflow isolation unit is connected to the electrochemical detection chamber, and the electrochemical detection chamber is connected to the waste liquid receiving area. The access determination module, the drive execution module, the positioning detection element, and the status detection module are electrically connected to the main control module to control the insertion, re-insertion trigger, sample transfer, anti-backflow isolation, electrochemical detection, and result output of the disposable sampling head.

2. The access interlock detection structure according to claim 1, characterized in that, The electrochemical detection chamber is equipped with a working electrode, a reference electrode, and an auxiliary electrode, which together constitute the electrochemical detection electrode system for liquid samples.

3. The access interlock detection structure according to claim 1, characterized in that, An interface cooperation and access interlock structure is provided between the disposable sampling head and the detection host. The interface cooperation and access interlock structure includes the detection host interface cavity, disposable sampling head, guide slope, limit step, insertion detection switch, identification contact, access interlock component, crimping actuator, sealing ring, sampling head outlet, detection chamber inlet, locking reset spring, anti-misinsertion block and positioning reference surface.

4. The access interlock detection structure according to claim 3, characterized in that, After the disposable sampling head is inserted into the interface cavity of the detection host, it is guided and limited by the guide slope and the limiting step. The insertion detection switch and identity recognition contact complete the insertion detection and identity verification. Under the action of the access interlock and the pressing actuator, the sampling head outlet is connected to the detection chamber inlet and sealed by the sealing ring.

5. The access interlock detection structure according to claim 1, characterized in that, The quantitative transfer and backflow prevention isolation structure of the disposable sampling head includes a sampling port, a sampling aspiration chamber, a quantitative metering chamber, a back-insertion trigger column, a push-transfer element, a one-way isolation valve, a backflow prevention buffer chamber, an electrochemical detection chamber inlet, a waste liquid bypass, an exhaust port, and a liquefactive membrane. At least one liquefactive membrane is provided on the exhaust path, forming a guiding path for guiding the sample from the sampling port to the electrochemical detection chamber inlet.

6. The access interlock detection structure according to claim 5, characterized in that, After entering the sampling aspiration chamber through the sampling port, the liquid sample enters the quantitative measurement chamber. When the disposable sampling head is reinserted, the reinsertion trigger column triggers the push-transfer device to push the quantitative sample downstream of the one-way isolation valve. It then enters the electrochemical detection chamber inlet through the anti-backflow buffer chamber. The remaining gas is discharged through the exhaust port, and the excess liquid is discharged through the waste liquid bypass.

7. The access interlock detection structure according to claim 1, characterized in that, The status detection module is used to detect the insertion status of the disposable sampling head, the quantitative sample transfer status, the backflow prevention isolation status, and the electrochemical detection chamber introduction status, and sends the detection results to the main control module and the result output module.

8. A detection method based on the access interlock detection structure according to any one of claims 1 to 7, characterized in that, The process includes the following steps: sampling head sampling; sampling head re-insertion; insertion in place detection; access verification; interlocking permission triggering; quantitative sample transfer; backflow prevention isolation; import into the detection chamber; status determination; when the status is normal, detection is allowed and the detection result is output after the detection is completed; when the status is abnormal, the exception is locked and a retry is prompted.

9. The detection method according to claim 8, characterized in that, The status determination includes at least a comprehensive determination of the sampling head insertion status, identity verification status, quantitative sample transfer status, backflow prevention isolation status, and electrochemical detection chamber introduction status. When any status does not meet the preset conditions, the detection process is locked and a retry prompt is output.

10. The detection method according to claim 8, characterized in that, The non-invasive liquid sample includes at least one of saliva, oral fluid, tears, sweat, or exhaled condensate. The test results are used to indicate the sample status, test result, or for retesting, and are not used as a conclusion for disease diagnosis.

Images