A method for promoting lateral root generation and improving saline-alkali tolerance of pears by using chlorogenic acid

Abstract

The application discloses a method for promoting lateral root generation of pears and improving salt and alkali tolerance by using chlorogenic acid. It relates to the field of agricultural technology. The method comprises the following steps: selecting pear seedling or transplanted seedling with consistent growth and no diseases and insect pests; and applying exogenous chlorogenic acid solution with a concentration of 1-10 micromoles per liter. The method can significantly improve the root system development quality: the number of lateral root primordia, the number of lateral roots, the total root length and the number of root tips are obviously increased, and the root system is more developed; in particular, 10 micromoles per liter of chlorogenic acid treatment can reduce the salt injury index of the plant, K + , Na + , and the steady state is maintained, the membrane damage is reduced, and the antioxidant capacity is improved; the method is natural, safe, green and environmentally friendly: the chlorogenic acid is a plant endogenous substance, has no residue and no pollution, and meets the ecological planting requirements; the method is simple to operate and low in cost, can effectively improve the survival rate of pear seedling transplantation, enhance the salt and alkali stress tolerance of pear seedling, and is suitable for large-scale application in seedling raising fields, orchards and saline-alkali lands.

Description

Technical Field

[0001] This invention relates to the field of agricultural technology, and more specifically to a method for promoting the development of lateral roots in pears and improving their salt and alkali tolerance using chlorogenic acid. Background Technology

[0002] Pears are one of my country's important fruit trees, with abundant varieties and high economic value. The pear industry is gradually expanding into saline-alkali land and marginal land. Cultivating pears and other economic fruit trees on saline-alkali land has become an important way to expand the development space of the fruit industry and improve ecological and economic benefits.

[0003] Pears are mainly propagated through grafting. Among them, the wild pear (Pyrus pyrifolia) exhibits good adaptability and resistance, and is widely used as rootstock for pear trees in northern and saline-alkali regions. However, the wild pear has a distinct taproot system with a well-developed main root and few lateral roots. This makes it prone to root damage during seedling lifting and transplanting, leading to reduced root recovery capacity. Furthermore, root growth is significantly inhibited in saline-alkali environments, directly resulting in a long recovery period after transplanting, slow root establishment, and a drastically reduced survival rate. Simultaneously, poor root development in saline-alkali environments further exacerbates ion imbalance, oxidative damage, and osmotic stress, leading to weak plant growth and difficulty adapting to the harsh environment of saline-alkali soils.

[0004] Currently, most rooting agents used in production are auxins, which can only promote root growth and cannot significantly improve the plant's salt and alkali tolerance. They are ineffective and lack stability in saline-alkali environments. Furthermore, saline-alkali soil improvement techniques cannot fundamentally address the problems of few lateral roots and low transplant survival rates in pear trees. Therefore, a green and safe regulatory technology that can simultaneously promote lateral root development, improve transplant survival rates, and enhance salt and alkali tolerance remains a gap in the market.

[0005] Chlorogenic acid (CGA) is a natural phenolic acid that is widely found in plants. It is safe, non-toxic, and environmentally friendly, and has functions such as anti-oxidation, free radical scavenging, anti-inflammatory and bactericidal effects. It is currently mainly used in the fields of food, medicine and cosmetics.

[0006] Therefore, whether a method can be provided to promote the development of lateral roots in pears and improve their salt and alkali tolerance using chlorogenic acid, and overcome the above-mentioned technical deficiencies, is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0007] In view of this, the present invention provides a method for promoting lateral root development and improving salt and alkali tolerance in pear cultivation using chlorogenic acid. This invention addresses the key technical bottlenecks in pear cultivation in saline-alkali soils, such as insufficient lateral root development, low transplant survival rate, and poor salt and alkali tolerance. It can significantly improve root development quality: the number of lateral root primordia, the number of lateral roots, the total root length, and the number of root tips are significantly increased, resulting in a more developed root system; in particular, treatment with 10 μmol / L chlorogenic acid can reduce the plant's salt damage index and K0. + Na+ It maintains stable conditions, reduces membrane damage, and enhances antioxidant capacity; it is natural, safe, and environmentally friendly: chlorogenic acid is an endogenous plant substance, leaving no residue or pollution, meeting the requirements of ecological planting; it is simple to operate and low in cost, effectively improving the survival rate of pear seedlings after transplanting and enhancing their tolerance to salt and alkali stress, making it suitable for large-scale application in nurseries, orchards, and saline-alkali land. It provides efficient, safe, and scalable technical support for the high-quality ecological development of the pear industry in saline-alkali land.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] As can be seen from the above technical solution, compared with the prior art, the present invention discloses a method for promoting the development of lateral roots in pear and improving salt and alkali tolerance using chlorogenic acid, and the technical effects achieved are as follows: 1. (Exogenous application of chlorogenic acid solution at a concentration of 1–10 μmol / L) significantly improves root development quality: the number of lateral root primordia, the number of lateral roots, the total root length, and the number of root tips are significantly increased, resulting in a more developed root system; 2. Enhanced salt and alkali tolerance: Under salt stress conditions, treatment with 10 μmol / L chlorogenic acid reduced the salt damage index and K0.05 + Na + Maintaining homeostasis, reducing membrane damage, and improving antioxidant capacity; 3. Natural, safe, green and environmentally friendly: Chlorogenic acid is an endogenous substance in plants, with no residue and no pollution, meeting the requirements of ecological planting; 4. It is simple to operate and low in cost, making it suitable for large-scale application in seedling nurseries, orchards, and saline-alkali land. Attached Figure Description

[0010] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0011] Figure 1 The attached figure shows the effect of different concentrations of CGA provided by this invention on the root structure of seedling pear under different conditions; where A: total root length; B: total root surface area; C: total number of root tips; D: total number of root forks.

[0012] Figure 2 The attached figure shows the effects of exogenous CGA and AOPP treatments provided by this invention on the aboveground and underground phenotypes of pear clone seedlings; wherein, A: the effect of CGA and AOPP treatments on pear clone seedlings under normal growth conditions; B: the effect of CGA and AOPP treatments on pear clone seedlings under salt stress.

[0013] Figure 3 The attached figure shows the effects of CGA and AOPP treatments provided by this invention on the aboveground phenotype of pear clone seedlings; where A: plant height; B: crown width; C: chlorophyll content.

[0014] Figure 4 The attached figure shows the effect of CGA and AOPP treatments provided by this invention on the photosynthetic index of pear clone seedlings, where A: net photosynthetic rate; B: intercellular CO2 concentration; C: transpiration rate; and D: stomatal conductance.

[0015] Figure 5 The attached figure shows the effects of CGA and AOPP treatments provided by this invention on the root structure of pear clonal seedlings, where A: total root length; B: total root surface area; C: total root volume; D: average diameter; E: total number of root tips; F: total number of root forks.

[0016] Figure 6 The attached figure shows the effect of CGA treatment provided by the present invention on the aboveground and belowground parts of 'Qingzhen V111'; where A and B represent the contents of endogenous GA4 and IAA in the aboveground part; and C to F represent the contents of endogenous IP, cZ, tZ, and DHZR in the belowground part.

[0017] Figure 7 The attached figure shows the effects of exogenous CGA and 6-BA treatments provided by this invention on the aboveground and underground phenotypes of pear clone seedlings; wherein, A: the effect of CGA and 6-BA treatments on pear clone seedlings under normal growth conditions; B: the effect of CGA and 6-BA treatments on pear clone seedlings under salt stress conditions.

[0018] Figure 8 The attached figure shows the effects of CGA and 6-BA treatments provided by this invention on the aboveground phenotype of pear clone seedlings; where A: plant height; B: crown width; C: chlorophyll content.

[0019] Figure 9 The attached figure shows the effect of CGA and 6-BA treatments provided by this invention on the photosynthetic index of pear clone seedlings, where A: net photosynthetic rate; B: intercellular CO2 concentration; C: transpiration rate; and D: stomatal conductance.

[0020] Figure 10 The attached figure shows the effect of CGA and 6-BA treatments provided by this invention on the root structure of pear clonal seedlings; where A: total root length; B: total root surface area; C: total root volume; D: average diameter; E: total number of root tips; F: total number of root forks.

[0021] Figure 11The attached figure shows the effects of CGA and 6-BA treatments provided by this invention on pear plants under salt stress, where A and B represent the Na+ and K+ content in leaves, and C and D represent the Na+ and K+ content in roots.

[0022] Figure 12 The attached figure shows the effects of CGA and 6-BA treatments provided by this invention on *Pyrus pyrifolia* seedlings under salt stress, where A~D: O3 content in leaves. 2- Contents of H2O2, proline, and malondialdehyde; E~H: O in roots 2- Content of H2O2, proline and malondialdehyde.

[0023] Figure 13 The attached figure shows the effect of CGA and 6-BA treatments provided by the present invention on the antioxidant enzyme activities in pear clones under salt stress, wherein A~C: leaf POD activity, leaf SOD activity, leaf CAT activity; D~F: root POD activity, root SOD activity, root CAT activity. Detailed Implementation

[0024] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0025] This invention discloses a method for promoting the development of lateral roots in pear and improving its salt and alkali tolerance using chlorogenic acid.

[0026] Example 1 A method for promoting lateral root development and improving salt and alkali tolerance in pear using chlorogenic acid includes the following steps: (1) Select pear seedlings or transplanted seedlings with uniform growth and free from pests and diseases for use; (2) Apply chlorogenic acid solution from an external source at a concentration of 1 μmol / L (using water as the solvent).

[0027] To further optimize the technical solution: Step (1) Pears include Du pear, Dou pear, and Qiuzi pear.

[0028] To further optimize the technical solution: when selecting pear seedlings in step (1), the process before step (1) also includes seed treatment and sowing: mix the cleaned pear seeds with moist river sand and store them in sand; after the sand storage is completed, sow the seeds and cultivate them to obtain pear seedlings with uniform growth.

[0029] To further optimize the technical solution: Step (2) Application: Apply by root irrigation.

[0030] To further optimize the technical solution: after step (2), step (3) salt stress treatment is also included: after chlorogenic acid solution treatment for 14 days, the pear seedlings are routinely maintained with clean water or subjected to 200 mM NaCl salt stress drenching treatment.

[0031] Example 2 A method for promoting lateral root development and improving salt and alkali tolerance in pear using chlorogenic acid includes the following steps: (1) Select pear seedlings or transplanted seedlings with uniform growth and free from pests and diseases for use; (2) Apply chlorogenic acid solution from an external source at a concentration of 5 μmol / L (using water as the solvent).

[0032] To further optimize the technical solution: Step (1) Pears include Du pear, Dou pear, and Qiuzi pear.

[0033] To further optimize the technical solution: when selecting pear seedlings in step (1), the process before step (1) also includes seed treatment and sowing: mix the cleaned pear seeds with moist river sand and store them in sand; after the sand storage is completed, sow the seeds and cultivate them to obtain pear seedlings with uniform growth.

[0034] To further optimize the technical solution: Step (2) Application: Apply by root irrigation.

[0035] To further optimize the technical solution: after step (2), step (3) salt stress treatment is also included: after chlorogenic acid solution treatment for 14 days, the pear seedlings are routinely maintained with clean water or subjected to 200 mM NaCl salt stress drenching treatment.

[0036] Example 3 A method for promoting lateral root development and improving salt and alkali tolerance in pear using chlorogenic acid includes the following steps: (1) Select pear seedlings or transplanted seedlings with uniform growth and free from pests and diseases for use; (2) Apply chlorogenic acid solution from an external source at a concentration of 10 μmol / L (using water as the solvent).

[0037] To further optimize the technical solution: Step (1) Pears include Du pear, Dou pear, and Qiuzi pear.

[0038] To further optimize the technical solution: when selecting pear seedlings in step (1), the process before step (1) also includes seed treatment and sowing: mix the cleaned pear seeds with moist river sand and store them in sand; after the sand storage is completed, sow the seeds and cultivate them to obtain pear seedlings with uniform growth.

[0039] To further optimize the technical solution: Step (2) Application: Apply by root irrigation.

[0040] To further optimize the technical solution: after step (2), step (3) salt stress treatment is also included: after chlorogenic acid solution treatment for 14 days, the pear seedlings are routinely maintained with clean water or subjected to 200 mM NaCl salt stress drenching treatment.

[0041] Example 4 1. Effects of different concentrations of chlorogenic acid on root structure and salt-alkali stress tolerance of pear plants Seed treatment and sowing: Washed pear seeds were mixed with moist river sand at a mass ratio of 1:3 and stratified in a sand stratification environment at 2.5℃ for 75 days. After stratification, the seeds were sown and placed in a greenhouse for cultivation. The greenhouse cultivation conditions were: temperature 25℃ and relative humidity 65%. Experimental grouping and treatment: One-month-old pear seedlings with uniform growth status were randomly divided into 5 groups: water (control), 1 μM CGA, 10 μM CGA, 100 μM CGA, and 1000 μM CGA. All treatments were administered by root irrigation, with a dosage of 50 mL per seedling. After 14 days of treatment, the experimental materials in the above 5 groups were treated with water (control) and 200 mM NaCl, respectively, with a dosage of 50 mL per seedling. Treatment was repeated every 7-10 days depending on soil water loss. Each treatment group had 3 replicates, with 10 seedlings per replicate. Phenotypic observation and index determination: After 30 days of salt treatment, the growth of *Pyrus pyrifolia* seedlings was observed. The root system of each group of seedlings was scanned and analyzed using a root scanner to determine the total root length, number of root tips, total root area, number of root branches, and other indicators.

[0042] Experimental results: Treatment with 1 μM and 10 μM chlorogenic acid significantly increased the number of lateral roots in *Pyrus pyrifolia* plants, with the 10 μM chlorogenic acid treatment showing the best effect. Root scanning analysis showed that the total root length, number of root tips, total root area, and number of root branches in both treatment groups were significantly higher than those in the water control group. When the chlorogenic acid concentration reached 100 μM and 1000 μM, it significantly inhibited the growth of *Pyrus pyrifolia* seedlings, with the number of lateral roots and various root indicators lower than those in the control group. Salt treatment significantly inhibited root development, significantly reducing the total root surface area, number of root tips, and number of root branches, while exogenous application of 1 μM and 10 μM chlorogenic acid significantly increased the number of root tips, and 10 μM chlorogenic acid also significantly increased the total root surface area and number of root branches. Figure 1 The above results indicate that, under both normal growth conditions and salt stress treatment, root irrigation with exogenous 1-10 μM, especially 10 μM chlorogenic acid, can significantly promote lateral root development and alter root structure.

[0043] 2. Determination of plant growth status and related physiological indicators under 10 μM chlorogenic acid treatment After transplanting, the pear clones were placed in a greenhouse for cultivation. The greenhouse cultivation conditions were: temperature 25±2℃, relative humidity 60%-70%. Pear clones with a seedling age of one month and uniform growth status were selected and randomly divided into four groups: a water control group, a 10 μM CGA treatment, a 1 μM AOPP (chlorogenic acid synthesis inhibitor) treatment, and a co-treatment of 10 μM CGA and 1 μM AOPP. All treatments were administered via root irrigation, with a dosage of 50 mL per plant. After 14 days of treatment, the experimental materials in the above four groups were subjected to root irrigation with water (control) and 200 mM NaCl, respectively, with a dosage of 50 mL per plant. Treatment was repeated every 7-10 days depending on soil water loss. Each treatment group had three replicates, with 10 seedlings per replicate. Phenotypic observation and index determination: After 30 days of salt treatment, the growth of pear plants was observed, and plant height, crown width, chlorophyll content, photosynthetic index, etc. were measured. The root system of each group of seedlings was scanned and analyzed using a root scanner to measure the total root length, number of root tips, total root area, number of root branches, and other indicators.

[0044] Experimental results: 10 μM CGA significantly promoted plant height, crown width, chlorophyll content, and photosynthetic indicators such as net photosynthetic rate, intercellular CO2 concentration, transpiration rate, and stomatal conductance under normal growth conditions. It also altered root structure, significantly increasing the number of lateral roots, total root surface area, total root volume, and total root length. AOPP treatment significantly inhibited all of the above indicators, but CGA+AOPP treatment allowed for the recovery of these inhibited indicators. Salt treatment significantly inhibited aboveground growth, leaf photosynthetic capacity, and root development, but CGA still promoted plant growth and photosynthetic capacity, and increased the number of roots, especially lateral roots. AOPP exacerbated the inhibitory effect of salt stress on the above indicators, while CGA+AOPP treatment partially recovered the inhibitory effect of AOPP on the aboveground and underground indicators. Figure 2-5 ).

[0045] 3. Determination of endogenous hormone levels under CGA treatment After transplanting, the pear clones were placed in a greenhouse for cultivation. The greenhouse cultivation conditions were: temperature 25±2℃, relative humidity 60%-70%. Pear clones with a seedling age of 1 month and uniform growth status were selected and treated with water (control) and 10 μM CGA respectively. All treatment groups were implemented by root irrigation, with an irrigation volume of 50 mL / plant. Fourteen days later, aboveground and underground tissues of the plant were collected, and the endogenous hormones in the plant tissues were quantitatively analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS).

[0046] Experimental results: The results showed that CGA treatment significantly increased the GA4 and IAA content in the aboveground tissues ( Figure 6 AB), and significantly reduced the content of active cytokinins such as IP, cZ, and DHZR in the roots ( Figure 6 CF).

[0047] 4. Cytokinin-mediated effects of CGA on plant root structure and salt stress tolerance 1. After transplanting the pear clones, they were placed in a greenhouse for cultivation. The greenhouse cultivation conditions were: temperature 25±2℃, relative humidity 60%-70%; 2. Pear clones with a seedling age of one month and uniform growth status were selected and randomly divided into four groups: a water control group, a treatment group treated with 10 μM CGA, a treatment group treated with 2 mg / L 6-BA, and a treatment group treated with 10 μM CGA + 2 mg / L 6-BA. All treatments were administered by root irrigation, with a dosage of 50 mL per plant. After 14 days of treatment, the experimental materials of the above four groups were treated by root irrigation with water (control) and 200 mM NaCl, respectively, with a dosage of 50 mL per plant. Treatments were repeated every 7-10 days depending on soil water loss. Each treatment group had three replicates, with 10 seedlings per replicate. 3. Phenotypic observation and index determination: After 30 days of salt treatment, the growth of pear plants was observed, and plant height, crown width, chlorophyll content, photosynthetic index, salt damage index, survival rate, etc. were measured. The root system of each group of seedlings was scanned and analyzed using a root scanner to measure the total root length, number of root tips, total root area, number of root branches, and other indicators.

[0048] 4. Measurement of physiological indicators related to salt and alkali tolerance, including K + Na + Content of MDA, H2O2, O2 - Content, activity of antioxidant enzymes such as POD and CAT, etc.

[0049] Experimental results: 10 μM CGA significantly promoted plant height, crown width, chlorophyll content, and photosynthetic indicators such as net photosynthetic rate, intercellular CO2 concentration, transpiration rate, and stomatal conductance under normal growth conditions. It also altered root structure, significantly increasing the number of lateral roots, total root surface area, total root volume, and total root length. Figure 7-106-BA treatment significantly inhibited all of the above indicators, but CGA+6-BA treatment could restore the inhibited indicators. Salt treatment significantly inhibited aboveground growth, leaf photosynthetic capacity, and root development, but CGA treatment under salt stress could still promote aboveground growth, photosynthetic capacity, and the increase in the number of roots, especially lateral roots. 6-BA aggravated the inhibitory effect of salt stress on aboveground growth, photosynthetic capacity, and root development, while CGA+6-BA treatment could partially restore the inhibitory effect of 6-BA on the above indicators. Figure 7-10 ).

[0050] Based on the statistical analysis of the aboveground phenotypes of plants under salt treatment, the salt damage index and plant survival rate were statistically analyzed. The control group showed no salt damage and a survival rate of 100%. However, under NaCl treatment, the salt damage index was as high as 79.2%, and the survival rate was only 58.3%. NaCl + CGA treatment significantly alleviated salt damage: the salt damage index decreased to 52.1%, and the survival rate recovered to 100%, indicating that CGA has a significant alleviating effect on salt stress damage in *Pyrus pyrifolia*. NaCl + 6-BA treatment exacerbated the salt stress damage: the salt damage index increased to 87.5%, and the survival rate further decreased to 41.7%, indicating that 6-BA enhances the sensitivity of *Pyrus pyrifolia* to salt stress. The salt damage index (81.3%) and survival rate (50.0%) of the NaCl + CGA + 6-BA combined treatment were similar to those of the NaCl group (Table 1). Based on these results, it is speculated that cytokinin mediates the effect of CGA on lateral root growth and salt stress tolerance.

[0051]

[0052] According to Na under each treatment + and K + Content determination results ( Figure 11 It was found that under normal conditions, CGA and 6-BA treatments affected the Na+ levels in plants. + It had no significant effect on the content, but it could increase the plant's potassium content. + Content, 6-BA treatment reduced plant K content + The content, co-treatment with CGA and 6-BA can reduce K + The Na+ content recovered to the control level; under salt stress treatment, the aboveground and underground Na+ content of the plants... + The content increased significantly, K + The content was significantly reduced; CGA treatment can reduce Na content. + And improve K + The content of Na increased significantly with 6-BA treatment. + And reduce K + The content, while co-treatment with CGA and 6-BA can reduce K + and Na +The content was restored; these results indicate that the CGA-CTK module may increase plant salt stress tolerance by regulating ion balance.

[0053] According to each treatment O 2- The levels of H2O2, proline, and malondialdehyde were found to be significantly reduced in both aboveground and belowground parts under normal growth conditions. - The content of H2O2, MDA and Pro; 6-BA treatment leads to O2 - The contents of H2O2, MDA, and Pro increased significantly; however, in the group co-treated with CGA and 6-BA, 6-BA reduced the effect of CGA on O2. - The inhibitory effects of H2O2, MDA, and Pro reduced the levels of various indicators back to near normal. Under salt stress, 'Qingzhen V111' suffered severe oxidative damage, resulting in a significant decrease in O2 levels in both the aboveground and underground parts. - The contents of H2O2, MDA, and Pro increased sharply; the application of exogenous CGA significantly inhibited the accumulation of ROS and greatly reduced the contents of MDA and Pro; the exogenous 6-BA treatment aggravated the salt stress-induced oxidative stress, resulting in a significant increase in the contents of ROS, MDA, and Pro; when CGA and 6-BA were applied together, the various oxidative damage indicators showed a slight increase compared with the CGA treatment alone, and the degree of damage was aggravated. Figure 12 ).

[0054] Based on the results of antioxidant enzyme activity assays under various treatments, it was found that under normal growth conditions, CGA treatment significantly increased the activities of POD and CAT in the leaves and roots of 'Qingkan V111', while SOD activity remained stable. 6-BA treatment significantly inhibited POD and CAT activities. Co-treatment with both treatments significantly alleviated the inhibitory effect of 6-BA, and enzyme activities were basically restored to the control group level. Under salt stress, the activities of antioxidant enzymes in 'Qingkan V111' increased to varying degrees, initiating antioxidant defense, but this was insufficient to completely offset the oxidative damage caused by the stress. Exogenous CGA further significantly increased POD and CAT activities, while SOD showed no significant difference. 6-BA significantly inhibited the activity of antioxidant enzymes. However, co-treatment with both treatments with 6-BA slightly inhibited the promoting effect of CGA. These results indicate that CGA primarily alleviates salt stress damage and enhances the tolerance of 'green anvil V111' by increasing the activity of POD and CAT enzymes in the antioxidant defense system and reducing ROS accumulation. Conversely, 6-BA inhibits the activity of antioxidant enzymes, leading to increased ROS and exacerbating salt damage. Furthermore, 6-BA weakens the salt stress-alleviating effect of CGA. Figure 13 ).

[0055] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0056] Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for promoting lateral root development and improving salt and alkali tolerance in pear using chlorogenic acid, characterized in that, Includes the following steps: (1) Select pear seedlings or transplanted seedlings with uniform growth and free from pests and diseases for use; (2) Apply chlorogenic acid solution from external sources at a concentration of 1 to 10 μmol / L.

2. The method as described in claim 1, characterized in that, The pears mentioned include wild pear, bean pear, and autumn pear.

3. The method as described in claim 1, characterized in that, When selecting pear seedlings in step (1), the process before step (1) also includes seed treatment and sowing: the cleaned pear seeds are mixed with moist river sand and stored in sand; after the sand storage is completed, the seeds are sown and cultivated to obtain pear seedlings with uniform growth.

4. The method as described in claim 2, characterized in that, The application in step (2) is carried out by root irrigation.

5. The method as described in claim 1, characterized in that, After step (2), step (3) salt stress treatment is also included: after 14 days of treatment with chlorogenic acid solution, the pear seedlings are routinely maintained with clean water or subjected to root drenching treatment with 200 mM NaCl salt stress.

6. The application of the method according to any one of claims 1 to 5 in agricultural production.

7. The application as described in claim 6, characterized in that, The method described above is used to improve the survival rate of pear seedlings after transplanting and to enhance their salt and alkali tolerance.

8. The application as described in claim 7, characterized in that, It is used to promote the formation of lateral root primordia in pears, increase the number of lateral roots, total root length, and number of root tips.

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