An ovarian tissue freezing device and method thereof

Abstract

The application discloses an ovary tissue freezing device and method thereof, and relates to the technical field of ovary tissue freezing, which comprises a freezing box, a top frame is installed above the freezing box through four vertical stand poles, a sealing box cover which is matched with the opening of the freezing box is arranged below the top frame, an outer cover is arranged on the top of the sealing box cover, and a controller two is connected with the rotating mechanism for controlling the rotating angle of the mesh metal clamp. The application realizes the precise regulation and control of the ovary tissue freezing process by combining mechanical linkage with intelligent control, drives the mesh metal clamp to ascend and descend by using a stepping motor, a transmission mechanism and a lifting mechanism, and cooperates with infrared sensing and a timing module to precisely control the immersion time, so that the residence time of the tissue in the freezing liquid is ensured to be consistent. Meanwhile, the rotating mechanism drives the metal clamp to reciprocally rotate within a range of 180 degrees, and the angle constraint of the limit switch is combined, so that each part of the tissue is uniformly contacted with the freezing liquid, cell damage caused by local uneven freezing is avoided, and the stability of the freezing quality is improved.

Description

Technical Field

[0001] This invention relates to the field of ovarian tissue cryopreservation technology, specifically to an ovarian tissue cryopreservation device and method. Background Technology

[0002] Ovarian reserve is a major limiting factor for female fertility. Natural aging, premature ovarian failure, insufficient blood supply to the ovaries due to pelvic surgery, and hereditary diseases all contribute to a gradual decline in ovarian function. Among these, the most significant damaging effects are seen in cancer patients treated with radiotherapy and chemotherapy. In recent years, the incidence of various cancers has been rising and showing a trend towards affecting younger women. Advances in cancer treatment have improved the survival rate of cancer patients, and the fertility issues of young cancer patients, as a crucial factor influencing their quality of life, have received increasing attention. Reproductive cryopreservation technology makes fertility preservation possible for high-risk patients with premature ovarian failure. Research and optimization of clinical techniques for fertility preservation hold promise for providing more options for high-risk patients with premature ovarian failure and older patients who wish to have children.

[0003] Current ovarian tissue freezing procedures largely rely on manual operation or simple timing devices to control the tissue's residence time in the cryoprotectant solution. Due to delays in manual operation or errors in mechanical transmission, the actual immersion time often deviates by more than ±10 seconds. This time difference leads to inconsistent concentrations of cryoprotectant within the tissue, resulting in inconsistent rates of intracellular ice crystal formation during rapid cooling. Insufficient immersion time may cause cell rupture and damage due to inadequate freezing, while excessive time may affect cell viability due to the accumulation of cryoprotectant toxicity, ultimately impacting the quality of surviving follicles after tissue thawing. Furthermore, most current ovarian tissue freezing procedures employ static placement of samples. Ovarian tissue often adheres to the container wall due to gravity, leading to insufficient contact between localized areas and the cryoprotectant solution, creating freezing "dead zones." In some areas, differences in cooling rates cause abnormal ice crystal growth, damaging the cytoskeleton and organelles, reducing the tissue's proliferative capacity after thawing, and in severe cases, leading to freezing failure.

[0004] Therefore, it is necessary to invent an ovarian tissue freezing device and method to solve the above problems. Summary of the Invention

[0005] The purpose of this invention is to provide an ovarian tissue freezing device and method to solve the problems in the prior art where the immersion time of ovarian tissue is not precisely controlled and the tissue is not uniformly in contact with the freezing solution when frozen with sequential freezing solution, which leads to easy damage to the sample.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an ovarian tissue freezing device and method, comprising a freezing chamber, a top frame mounted on the top of the freezing chamber via four vertical poles, a stepper motor mounted on one side of the top surface of the top frame, the output end of the stepper motor being sequentially connected to a reducer and a transmission mechanism, a sealing cover adapted to the opening of the freezing chamber being provided below the top frame, the transmission mechanism being connected to the sealing cover via a lifting mechanism to realize the lifting action of the sealing cover, two mesh metal clips for placing ovarian tissue being symmetrically suspended from the bottom surface of the sealing cover, an infrared sensor being provided on the inner wall of the freezing chamber, an infrared receiver being provided between the mesh metal clips, a timing module being provided on the outer wall of the freezing chamber, a controller one being provided on the side wall of the top frame and electrically connected to the stepper motor, the infrared sensor, and the timing module, the top of the mesh metal clips being rotatably connected to the sealing cover via a rotating plate, an outer shell being provided on the top of the sealing cover, a rotating mechanism being provided inside the outer shell, and a controller two being connected to the rotating mechanism for controlling the rotation angle of the mesh metal clips.

[0007] Preferably, the transmission mechanism includes a main gear, a secondary gear, and a toothed belt. The main gear is connected to the output end of the reducer. The secondary gear is rotatably mounted on the bottom surface of the top frame and is horizontally symmetrical with the main gear. The toothed belt is tensioned and sleeved between the main gear and the secondary gear to form synchronous transmission.

[0008] Preferably, the lifting mechanism includes two parallel reciprocating screws, the top ends of which are respectively connected to the center of the main gear and the auxiliary gear, and the bottom ends are rotatably connected to the outer wall of the freezer through bearing seats. A nut sleeve is threaded on the reciprocating screw, and the nut sleeve is fixedly connected to the side wall of the sealing box cover.

[0009] Preferably, the sealed box cover is provided with sliding sleeves on both sides that slide with the uprights. The mesh metal clips are driven to immerse or detach from the coolant by a lifting mechanism, and the immersion time is controlled by a timing module.

[0010] Preferably, the mesh metal clip includes a metal frame and a frame door. The metal frame is hinged to the frame door on one side and connected to the frame door on the other side by three evenly distributed latches. The metal frame has multiple storage slots arranged in an equidistant array, and the multiple storage slots are provided with elastic pressure strips corresponding to the positions of the frame door.

[0011] Preferably, the rotating mechanism includes a motor installed on the inner wall of the outer casing, a rotating shaft fixed to the output end of the motor, a connecting rod connected to one end of the rotating shaft, a connecting rod hinged to the end of the connecting rod, and a rotating shaft fixed to the bottom end of the connecting rod.

[0012] Preferably, the rotating mechanism further includes a driving gear and a driven gear, and the driving gear meshes with the driven gear. The eccentric end face of the driving gear is connected to the second rotating shaft. The second rotating shaft passes through the sealing cover and is connected to the rotating plate, driving the mesh metal clamp to rotate through gear transmission.

[0013] Preferably, the drive gear is provided with limit rods symmetrically distributed at 180° on both sides, the drive gear is provided with a metal pointer, the side wall of the limit rod is provided with a limit switch adapted to the metal pointer, and the controller is electrically connected to the motor and the limit switch to limit the rotation angle range of the mesh metal clamp.

[0014] Preferably, it includes the following steps: S1: Sample loading: Open the frame door of the mesh metal clip, place the ovarian tissue samples into the storage slots of the metal frame, close the frame door and lock it with the latches so that the elastic strips can stably limit the position of the samples; S2: Freezing preparation: The immersion time of the refrigerant in the mesh metal clip is preset by the timer module, and the controller is initialized and put into standby mode; S3: Lifting control: When the controller starts the stepper motor, the transmission mechanism is driven to run through the reducer. Through the synchronous transmission of the main gear, the auxiliary gear and the toothed belt, the reciprocating screw of the lifting mechanism is rotated, so that the nut sleeve drives the sealing box cover to descend along the upright until the mesh metal clip is completely immersed in the refrigerant in the freezer. At this time, the infrared sensor and the infrared receiver are triggered, and the controller records the immersion time and starts timing. S4: Rotation control: During the immersion time, controller two starts the motor of the rotation mechanism, which drives linkage one and linkage two through shaft one, so that shaft two drives the drive gear to swing back and forth. Through the driven gear, the rotating plate and the mesh metal clamp rotate. When the metal pointer on the drive gear touches the limit switch on the limit rod, controller two controls the motor to reverse, so that the mesh metal clamp can rotate back and forth within a range of 180°, ensuring that the sample is in uniform contact with the cryosol. S5: Removal from coolant: When the timer reaches the preset time, the controller controls the stepper motor to reverse, and the lifting mechanism drives the sealing box cover and mesh metal clamp to rise and remove from the coolant until the sealing box cover closes the opening of the freezer box, completing the initial coolant immersion process.

[0015] The technical effects and advantages provided by the present invention in the above technical solution are as follows: 1. This invention achieves precise control of the immersion time of the mesh metal clamp by combining multi-stage mechanical transmission with intelligent sensing. The stepper motor drives the reciprocating screw to rotate through the reducer and transmission mechanism, which drives the sealing box cover and the mesh metal clamp to rise and fall smoothly. With the triggering of the infrared sensor and infrared receiver, the immersion time can be accurately recorded. The timing module is linked with the controller and automatically drives the lifting mechanism to reset after the preset time is reached, so that the immersion time error is controlled within ±2 seconds. This solves the problem of inconsistent immersion time during manual operation and ensures the standardization of the freezing process. 2. This invention achieves stable control of the rotation angle of the mesh metal clip through a rotating mechanism and limit control. The motor drives the active gear to eccentrically swing through the linkage assembly, and drives the rotating plate to rotate through gear meshing, so that the mesh metal clip reciprocates within a range of 180°. The metal pointer on the active gear cooperates with the limit switch on the limit rod. Combined with the real-time feedback of the controller, it ensures that the rotation angle deviation does not exceed ±5°, ensuring that all parts of the ovarian tissue are evenly contacted with the cryosol, avoiding tissue activity damage caused by local freezing rate differences, and improving the uniformity of the freezing effect. 3. This invention features multiple equidistant arrays of storage slots within a mesh metal frame, which, together with the elastic pressure strip on the frame door, allows for the simultaneous and stable loading of multiple ovarian tissue samples. This solves the problem of limited single-load capacity in traditional containers. This structure enables the simultaneous processing of large batches of samples under the same freezing environment, avoiding the time lag associated with manual piece-by-piece handling, reducing the exposure time of tissues at room temperature, and minimizing cell viability loss due to temperature fluctuations. This provides a structural basis for improving resuscitation survival rates. Attached Figure Description

[0016] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a three-dimensional structural diagram of the transmission mechanism of the present invention; Figure 3 This is a schematic diagram of the three-dimensional structure of the mesh metal clip of the present invention; Figure 4 This is a schematic diagram of the three-dimensional structure of the infrared sensor of the present invention; Figure 5 This is a schematic diagram of the three-dimensional structure of the infrared receiver of the present invention; Figure 6This is a three-dimensional cross-sectional structural diagram of the outer casing of the present invention; Figure 7 This is a three-dimensional structural diagram of the rotating mechanism of the present invention; Figure 8 For the present invention Figure 7 Enlarged 3D structural diagram at point A; Figure 9 This is a schematic diagram of the process for controlling the freezing time according to the present invention; Figure 10 This is a schematic diagram of the process for controlling the rotation angle of the present invention; Figure 11 This is a schematic diagram of the improved AB groove design implementation in Embodiment 2 of the present invention.

[0018] Explanation of reference numerals in the attached figures: 1. Freezer; 2. Top rack; 3. Stepper motor; 4. Reducer; 5. Transmission mechanism; 501. Main gear; 502. Secondary gear; 503. Toothed belt; 6. Lifting mechanism; 601. Reciprocating screw; 602. Nut sleeve; 603. Sliding sleeve; 7. Sealing lid; 8. Mesh metal clamp; 801. Metal frame; 802. Frame door; 803. Lock; 804. Storage slot; 805. Pressure strip; 9. Infrared sensor; 1 0. Infrared receiver; 11. Timing module; 12. Controller 1; 13. Rotating plate; 14. Outer casing; 15. Rotating mechanism; 1501. Motor; 1502. Rotating shaft 1; 1503. Connecting rod 1; 1504. Connecting rod 2; 1505. Rotating shaft 2; 1506. Driving gear; 1507. Driven gear; 1508. Limit rod; 1509. Metal pointer; 16. Limit switch; 17. Controller 2. Detailed Implementation

[0019] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0020] The components of the embodiments of the invention described and shown in the accompanying drawings can typically be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention.

[0021] Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0023] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0024] Example 1 This invention provides, for example Figure 1-10 An ovarian tissue freezing device and method are shown, comprising a freezing chamber 1, a top frame 2 mounted on the top of the freezing chamber 1 via four vertical poles, a stepper motor 3 on one side of the top surface of the top frame 2, the output of the stepper motor 3 being connected in sequence to a reducer 4 and a transmission mechanism 5, a sealing cover 7 adapted to the opening of the freezing chamber 1 being provided below the top frame 2, the transmission mechanism 5 being connected to the sealing cover 7 via a lifting mechanism 6 to realize the lifting action of the sealing cover 7, two mesh metal clips 8 for placing ovarian tissue being symmetrically suspended from the bottom surface of the sealing cover 7, an infrared sensor 9 being provided on the inner wall of the freezing chamber 1, an infrared receiver 10 being provided between the mesh metal clips 8, a timing module 11 being provided on the outer wall of the freezing chamber 1, a controller 12 electrically connected to the stepper motor 3, the infrared sensor 9, and the timing module 11 being provided on the side wall of the top frame 2, the top of the mesh metal clips 8 being rotatably connected to the sealing cover 7 via a rotating plate 13, an outer shell 14 being provided on the top of the sealing cover 7, a rotating mechanism 15 being provided inside the outer shell 14, the rotating mechanism 15 being connected to a controller 17 for controlling the rotation angle of the mesh metal clips 8.

[0025] In this embodiment, the stepper motor 3 serves as the power source, enabling precise control of speed and direction, providing stable power for the lifting and lowering of the sealing box lid 7. The cooperation between the transmission mechanism 5 and the lifting mechanism 6 achieves automated lifting and lowering of the sealing box lid 7, facilitating the placement and removal of ovarian tissue. Simultaneously, it ensures the sealing of the cryo-box 1 during the freezing process, preventing interference from the external environment on the freezing effect. Furthermore, the combination of the infrared sensor 9 and the infrared receiver 10 provides real-time feedback on ovarian tissue information, such as whether it is placed correctly and changes in position during the freezing process, providing accurate monitoring data for operators and ensuring the smooth progress of the freezing process. The cooperation between the timing module 11 and the controller 12 enables automated timing control of the freezing process. Different ovarian tissues may require different freezing times; the timing module 11 allows for flexible setting of the freezing duration, ensuring that the ovarian tissue is frozen within the optimal time, improving freezing quality and success rate. Simultaneously, combined with feedback information from the infrared sensor 9, the controller 12 can more precisely control the freezing process, avoiding any impact on the freezing effect due to inaccurate time control.

[0026] The transmission mechanism 5 includes a main gear 501, a secondary gear 502, and a toothed belt 503. The main gear 501 is connected to the output end of the reducer 4. The secondary gear 502 is rotatably mounted on the bottom surface of the top frame 2 and is horizontally symmetrical with the main gear 501. The toothed belt 503 is tensioned and sleeved between the main gear 501 and the secondary gear 502 to form synchronous transmission. The lifting mechanism 6 includes two parallel reciprocating screws 601. The top ends of the reciprocating screws 601 are respectively connected to the centers of the main gear 501 and the secondary gear 502. Their bottom ends are rotatably connected to the outer wall of the freezer 1 through bearing seats. Nut sleeves 602 are threaded on the reciprocating screws 601. Nut sleeves 602 are fixedly connected to the side wall of the sealing box cover 7. The sealing box cover 7 has sliding sleeves 603 on both sides that slide with the uprights. The lifting mechanism 6 drives the mesh metal clips 8 to be immersed in or detached from the refrigerant, and the immersion time is controlled by the timing module 11.

[0027] In this embodiment, when the transmission mechanism 5 drives the main gear 501 and the secondary gear 502 to rotate, the reciprocating screw 601 rotates accordingly. Due to the special thread structure of the reciprocating screw 601, the nut sleeve 602 will make up-and-down linear motion along the reciprocating screw 601. Since the nut sleeve 602 is fixedly connected to the sealing box cover 7, the sealing box cover 7 will move up and down along the upright under the drive of the nut sleeve 602. When the sealing box cover 7 moves downward, the mesh metal clip 8 will be immersed in the cryogenic liquid; when the sealing box cover 7 moves upward, the mesh metal clip 8 will be detached from the cryogenic liquid. Therefore, the raising and lowering of the sealing box cover 7 directly drives the movement of the mesh metal clip 8, realizing the operation of immersing or detaching the mesh metal clip 8 from the cryogenic liquid. With the help of the timing module 11, the immersion time of the mesh metal clip 8 in the cryogenic liquid can be precisely controlled to meet the freezing needs of different ovarian tissues and ensure the uniformity and consistency of the freezing effect.

[0028] The mesh metal clip 8 includes a metal frame 801 and a frame door 802. The metal frame 801 is hinged to the frame door 802 on one side and connected to the other side by three evenly distributed latches 803. The metal frame 801 has multiple storage slots 804 arranged in an equidistant array, and the multiple storage slots 804 are provided with elastic pressure strips 805 at the positions of the frame door 802.

[0029] In this embodiment, the mesh metal clip 8 is designed to facilitate the placement and fixation of ovarian tissue. The placement slot 804 and the elastic pressure strip 805 can ensure that the ovarian tissue will not shift or fall off during the freezing process, thus ensuring the uniformity and effectiveness of freezing.

[0030] The rotating mechanism 15 includes a motor 1501 mounted on the inner wall of the outer casing 14. A rotating shaft 1502 is fixed to the output end of the motor 1501. A connecting rod 1503 is connected to one end of the rotating shaft 1502. A connecting rod 1504 is hinged to the end of the connecting rod 1503. A rotating shaft 1505 is fixed to the bottom end of the connecting rod 1504. The rotating mechanism 15 also includes a driving gear 1506 and a driven gear 1507, with the driving gear 1506 meshing with the driven gear 1507. The end face of the driving gear 1506 is eccentrically positioned relative to the rotating shaft 1505. Shaft 2 1505 is connected, and shaft 2 1505 passes through the sealed box cover 7 and is connected to the rotating plate 13. It drives the mesh metal clamp 8 to rotate through gear transmission. The driving gear 1506 has limit rods 1508 symmetrically distributed at 180° on both sides. The driving gear 1506 has a metal pointer 1509. The side wall of the limit rod 1508 has a limit switch 16 adapted to the metal pointer 1509. Controller 2 17 is electrically connected to the motor 1501 and the limit switch 16 to limit the rotation angle range of the mesh metal clamp 8.

[0031] In this embodiment, the rotating mechanism 15 is designed to allow the mesh metal clip 8 to rotate during the freezing process, ensuring that the ovarian tissue is cooled evenly in the freezing solution and avoiding poor freezing results due to uneven local cooling. The combination of the limit rod 1508, the metal pointer 1509, and the limit switch 16 can precisely control the rotation angle range of the mesh metal clip 8, preventing over- or under-rotation and ensuring the accuracy and stability of the rotation action. The controller 17 can flexibly control the operation of the rotating mechanism 15 according to actual needs, realizing automated and precise rotation control.

[0032] Includes the following steps: Step 1: Sample loading: Open the frame door 802 of the mesh metal clip 8, place the ovarian tissue samples into the storage slots 804 of the metal frame 801, close the frame door 802 and lock it with the buckle 803, so that the elastic pressure strip 805 forms a stable limit on the sample. Step 2: Freezing preparation: The immersion time of the refrigerant in the mesh metal clip 8 is preset by the timer module 11, and the controller 12 is initialized and put into standby mode; Step 3: Lifting control: Controller 12 starts stepper motor 3, which drives transmission mechanism 5 through reducer 4. Through the synchronous transmission of main gear 501, secondary gear 502 and toothed belt 503, it drives the reciprocating screw 601 of lifting mechanism 6 to rotate, so that nut sleeve 602 drives the sealing box cover 7 to descend along the upright until the mesh metal clip 8 is completely immersed in the refrigerant in the freezer box 1. At this time, infrared sensor 9 and infrared receiver 10 are triggered, controller 12 records the immersion time and starts timing; Step 4: Rotation Control: During the immersion time, controller 2 17 starts the motor 1501 of the rotation mechanism 15, which drives the connecting rod 1503 and the connecting rod 2 1504 through the rotating shaft 1502, so that the rotating shaft 2 1505 drives the drive gear 1506 to swing back and forth. Through the driven gear 1507, the rotating plate 13 and the mesh metal clip 8 are rotated. When the metal pointer 1509 on the drive gear 1506 touches the limit switch 16 on the limit rod 1508, controller 2 17 controls the motor 1501 to reverse, so that the mesh metal clip 8 can rotate back and forth within a range of 180°, ensuring that the sample is in uniform contact with the cryosol. Step 5: Removal from the coolant: When the timer reaches the preset time, the controller 12 controls the stepper motor 3 to reverse, and the lifting mechanism 6 drives the sealing box cover 7 and the mesh metal clamp 8 to rise and remove the coolant until the sealing box cover 7 closes the opening of the freezer box 1, completing the initial immersion in the coolant. Working principle of this invention: Refer to the instruction manual appendix Figure 1-10 When using this invention, the stepper motor 3 is first started by the controller 12. The power output of the stepper motor 3 is reduced by the reducer 4 and then transmitted to the transmission mechanism 5. The main gear 501 in the transmission mechanism 5 rotates under the power drive, and drives the auxiliary gear 502 to rotate synchronously through the toothed belt 503. The main gear 501 and the auxiliary gear 502 respectively drive the two parallel reciprocating screws 601 to rotate. Due to the special thread structure of the reciprocating screw 601, the nut sleeve 602 moves upward in a straight line along the reciprocating screw 601, thereby driving the sealing box cover 7, which is fixedly connected to the nut sleeve 602, to move upward along the upright, so that the opening of the freezer 1 is opened, and the operator can open it. Open the frame door 802 of the mesh metal clip 8, unlock the three evenly distributed latches 803, open the frame door 802 along the hinge, and place the ovarian tissues that have been frozen for the first time and are to be frozen for the second time one by one into the multiple placement slots 804 that are evenly arranged inside the metal frame 801. Ensure that the ovarian tissues are placed stably. The elastic pressure strips 805 provided in the placement slots 804 corresponding to the positions of the frame door 802 will automatically exert a certain pressure on the ovarian tissues, fixing them in place in the placement slots 804 to prevent them from shifting or falling off during subsequent operations. Close the frame door 802 again, and fasten the three latches 803 to ensure that the mesh metal clip 8 is well sealed and the ovarian tissues are safely fixed inside. The stepper motor 3 is controlled to rotate in reverse by the controller 12 again, the transmission mechanism 5 and the lifting mechanism 6 run in reverse, the nut sleeve 602 moves downward in a straight line along the reciprocating screw 601, and drives the sealing cover 7 to move downward along the upright until the sealing cover 7 fits tightly with the opening of the freezer 1, sealing the freezer 1 and preventing the external environment from interfering with the freezing effect. As the sealing cover 7 continues to descend, the mesh metal clip 8 moves downward with the sealing cover 7 through the lifting mechanism 6 and is gradually immersed in the refrigerant in the freezer 1. When the sealing cover 7 descends to the appropriate position, when the mesh metal clip 8 is completely immersed in the refrigerant in the freezer 1, the infrared sensor 9 on the inner wall of the freezer 1 and the infrared receiver 10 between the mesh metal clip 8 are triggered. The controller 12 records the immersion time and starts timing. At this time, the controller 12 controls the stepper motor 3 to stop running. Then, the motor 1501 in the rotating mechanism 15 is started by the controller 17. The output end of the motor 1501 drives the rotating shaft 1502 to rotate. The rotating shaft 1502 drives the rotating shaft 1505 to reciprocate through the connecting rod 1503 and the connecting rod 1504. The rotating shaft 1505 is connected to the eccentric end face of the drive gear 1506, thereby driving the drive gear 1506 to reciprocate. The drive gear 1506 meshes with the driven gear 1507, transmitting power to the driven gear 1507, which in turn drives the rotating plate 13 to rotate through the rotating shaft 1505, causing the mesh metal clip 8 to rotate in the coolant. During the rotation, the metal pointer 1509 on the drive gear 1506 cooperates with the limit switch 16 on the limit rod 1508 which is symmetrically distributed at 180° on both sides to precisely control the rotation angle range of the mesh metal clip 8 and prevent over- or under-rotation. Simultaneously, the timing module 11 starts timing. When the preset freezing time is reached, the timing module 11 sends a signal to the controller 12. After receiving the signal from the timing module 11, the controller 12 controls the stepper motor 3 to rotate forward again. Through the transmission mechanism 5 and the lifting mechanism 6, the sealing box cover 7 is moved upward, thereby causing the mesh metal clip 8 to detach from the freezing liquid. The controller 2 stops the operation of the motor 1501 in the rotating mechanism 15, so that the mesh metal clip 8 stops rotating. After the sealing box cover 7 is fully opened, the operator opens the frame door 802 of the mesh metal clip 8 in the reverse order of placing the ovarian tissue, takes out the frozen ovarian tissue, and performs subsequent processing or preservation.

[0033] Example 2 As another embodiment of the present invention, see the appendix. Figure 11The cryobox is divided into a cryo-liquid tank A and a cryo-liquid tank B. Following the steps of Example 1, the mesh metal clip 8 is immersed in the cryo-liquid tank A. After immersion, it is raised. By setting a translation drive component, the mesh metal clip 8 is translated to the corresponding position above the cryo-liquid tank B. Then, the steps S2 to S5 are repeated. The timing module presets the cryo-liquid immersion time of the mesh metal clip 8, immerses the mesh metal clip 8 in the cryo-liquid tank B, and controls the rotation and lifting of the mesh metal clip 8. Example 2 is an improvement and adjustment made to the steps of Example 1, which can more closely approximate the actual cryo-freezing process of ovarian tissue. It should be noted that the mesh metal clip 8 is moved to the corresponding position above slot B by setting a translation drive component. The accompanying drawings of this invention do not show the translation drive component because the translation drive component is conventional prior art and does not need to be described in detail. For reference, you can refer to the translation drive component used in Chinese patents such as CN119664786A / CN103050130A.

[0034] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0035] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An ovarian tissue cryopreservation device, comprising a cryostat (1), characterized in that: A top frame (2) is installed above the freezer (1) via four vertical poles. A stepper motor (3) is provided on one side of the top surface of the top frame (2). The output end of the stepper motor (3) is connected to a reducer (4) and a transmission mechanism (5) in sequence. A sealing cover (7) adapted to the opening of the freezer (1) is provided below the top frame (2). The transmission mechanism (5) is connected to the sealing cover (7) via a lifting mechanism (6) to realize the lifting action of the sealing cover (7). Two mesh metal clips (8) for placing ovarian tissue are symmetrically suspended on the bottom surface of the sealing cover (7). An infrared sensor (9) is provided on the inner wall of the freezer (1). An infrared receiver (10) is provided between the mesh metal clips (8). A timing module (11) is provided on the outer wall of the freezer (1). A controller (12) is provided on the side wall of the top frame (2) and is electrically connected to the stepper motor (3), the infrared sensor (9), and the timing module (11). The top of the mesh metal clips (8) is rotatably connected to the sealing box cover (7) through a rotating plate (13). The top of the sealing box cover (7) is provided with an outer cover (14). A rotating mechanism (15) is provided inside the outer cover (14). The rotating mechanism (15) is connected to a controller (17) to control the rotation angle of the mesh metal clips (8).

2. The ovarian tissue cryopreservation device according to claim 1, characterized in that: The transmission mechanism (5) includes a main gear (501), a secondary gear (502) and a toothed belt (503). The main gear (501) is connected to the output end of the reducer (4). The secondary gear (502) is rotatably mounted on the bottom surface of the top frame (2) and is horizontally symmetrical with the main gear (501). The toothed belt (503) is tensioned and sleeved between the main gear (501) and the secondary gear (502) to form synchronous transmission.

3. The ovarian tissue cryopreservation device according to claim 1, characterized in that: The lifting mechanism (6) includes two parallel reciprocating screws (601). The top of the reciprocating screws (601) is connected to the center of the main gear (501) and the auxiliary gear (502) respectively. The bottom of the screws is rotatably connected to the outer wall of the freezer (1) through a bearing seat. A nut sleeve (602) is threaded on the reciprocating screws (601). The nut sleeve (602) is fixedly connected to the side wall of the sealing box cover (7).

4. The ovarian tissue cryopreservation device according to claim 3, characterized in that: The sealed box cover (7) is provided with sliding sleeves (603) on both sides that slide with the upright. The mesh metal clip (8) is driven by the lifting mechanism (6) to immerse or detach from the coolant, and the immersion time is controlled by the timing module (11).

5. An ovarian tissue cryopreservation device according to claim 4, characterized in that: The mesh metal clip (8) includes a metal frame (801) and a frame door (802). The metal frame (801) is hinged to the frame door (802) on one side and connected to the frame door (802) on the other side by three evenly distributed latches (803). The metal frame (801) has multiple storage slots (804) arranged in an equidistant array, and the multiple storage slots (804) are provided with elastic pressure strips (805) corresponding to the positions of the frame door (802).

6. The ovarian tissue cryopreservation device according to claim 1, characterized in that: The rotating mechanism (15) includes an electric motor (1501) installed on the inner wall of the outer casing (14). The output end of the electric motor (1501) is fixed with a rotating shaft (1502). One end of the rotating shaft (1502) is connected to a connecting rod (1503). The end of the connecting rod (1503) is hinged to a connecting rod (1504). The bottom end of the connecting rod (1504) is fixed with a rotating shaft (1505).

7. An ovarian tissue cryopreservation device according to claim 6, characterized in that: The rotating mechanism (15) further includes a driving gear (1506) and a driven gear (1507), and the driving gear (1506) meshes with the driven gear (1507). The end face of the driving gear (1506) is eccentrically connected to the second rotating shaft (1505). The second rotating shaft (1505) passes through the sealing box cover (7) and is connected to the rotating plate (13), driving the mesh metal clamp (8) to rotate through gear transmission.

8. An ovarian tissue cryopreservation device according to claim 7, characterized in that: The drive gear (1506) has limit rods (1508) symmetrically distributed at 180° on both sides. The drive gear (1506) has a metal pointer (1509). The side wall of the limit rod (1508) has a limit switch (16) adapted to the metal pointer (1509). The controller (17) is electrically connected to the motor (1501) and the limit switch (16) to limit the rotation angle range of the mesh metal clip (8).

9. A method for freezing ovarian tissue according to any one of claims 1-8, characterized in that, Includes the following steps: S1: Sample loading: Open the frame door (802) of the mesh metal clip (8), place the ovarian tissue samples into the storage slot (804) of the metal frame (801), close the frame door (802) and lock it with the buckle (803) so that the elastic pressure strip (805) forms a stable limit on the sample; S2: Freezing preparation: The freezing liquid immersion time of the mesh metal clip (8) is preset by the timing module (11), and the controller (12) is initialized and put into standby mode; S3: Lifting control: Controller 1 (12) starts the stepper motor (3), which drives the transmission mechanism (5) through the reducer (4). Through the synchronous transmission of the main gear (501), the auxiliary gear (502) and the toothed belt (503), the reciprocating screw (601) of the lifting mechanism (6) rotates, causing the nut sleeve (602) to drive the sealing box cover (7) to descend along the upright until the mesh metal clip (8) is completely immersed in the freezing liquid in the freezer (1). At this time, the infrared sensor (9) and the infrared receiver (10) are triggered, and controller 1 (12) records the immersion time and starts timing. S4: Rotation control: During the immersion time, controller two (17) starts the motor (1501) of the rotation mechanism (15), which drives the linkage one (1503) and linkage two (1504) through the rotating shaft one (1502), so that the rotating shaft two (1505) drives the active gear (1506) to swing back and forth, and drives the rotating plate (13) and the mesh metal clip (8) to rotate through the driven gear (1507). When the metal pointer (1509) on the active gear (1506) touches the limit switch (16) on the limit rod (1508), controller two (17) controls the motor (1501) to reverse, so that the mesh metal clip (8) can rotate back and forth within a range of 180°, ensuring that the sample is in uniform contact with the cryosol; S5: Removal from the coolant: When the timer reaches the preset time, the controller (12) controls the stepper motor (3) to reverse, and through the lifting mechanism (6) drives the sealing box cover (7) and the mesh metal clip (8) to rise and remove the coolant until the sealing box cover (7) closes the opening of the freezer box (1), completing the initial coolant immersion process.

10. A method for freezing ovarian tissue according to claim 9, characterized in that, It also includes the following steps: S6: Secondary immersion: The cryobox (1) is divided into cryo-liquid tank A and cryo-liquid tank B. After the mesh metal clip (8) is immersed in cryo-liquid tank A, the mesh metal clip (8) is moved to the corresponding position above cryo-liquid tank B by setting the translation drive component. The steps from S2 to S5 are repeated, that is, the cryo-liquid immersion time of the mesh metal clip (8) is preset by the timing module (11), the mesh metal clip (8) is lowered and immersed in cryo-liquid tank B, and the rotation and lifting of the mesh metal clip (8) are controlled to realize the sequential immersion and penetration of ovarian tissue in cryo-liquid tanks A and B.

Images